Istituto Zooprofilattico Sperimentale
della Lombardia e dell’Emilia Romagna
“Bruno Ubertini”
PUBLICATIONS FROM 2006 TO 2010
ID nr. 3163
Brocchi° E, Bergmann IE, Dekker A, Paton DJ, Sammin DJ, Greiner M, Grazioli° S,
De_Simone° F, Yadin H, Haas B, Bulut N, Malirat V, Neitzert E, Goris N, Parida S,
Sorensen K, De_Clercq K
Comparative evaluation of six ELISAs for the detection of antibodies to the non-structural proteins of
foot-and-mouth disease virus
Vaccine. - Vol. 24 no 47-48 ( 2006). - p 6966-6979. - 21 bib ref
Impact factor: 2,822
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
To validate the use of serology in substantiating freedom from infection after foot-and-mouth disease (FMD)
outbreaks have been controlled by measures that include vaccination, 3551 sera were tested with six assays
that detect antibodies lo the non-structural proteins of FMD virus. The sera came from naïve, vaccinated,
infected and vaccinated-and-infected animals two-thirds from cattle, the remainder from sheep and pigs. The
assays were covariant for sensitivity, but not necessarily for specificity. A commercial kit from Cedidiagnostica and an in-house assay from 1ZS-Brescia were comparable to the NCPanaftosa-screening index
method described in the Diagnostic Manual of the World Animal Health Organisation. Using these three tests
the specifìcity and sensitivity. for the detection of carriers in vaccinated cattle approaches or exceeds 99%
and 90% respectively.
ID nr. 3184
Stacchiotti A, Rodella LF, Ricci F, Rezzani R, Lavazza° A, Bianchi R
Stress proteins expression in rat kidney and liver chronically exposed to aluminium suiphate
Histol Histopathol. - Vol. 21 ( 2006). - p 131-140. - 64 bib ref
Impact factor: 2,023
Aluminium (Al) is the third most widespread metal in the environment. It is toxic for the brain, bone and
haematological system but unfortunately very little data exist for other organs. Stress proteins are induced or
enhanced against metal toxicity with an essential role in the recovery of organules and other cellular proteins.
This immunohistochemical study was performed to analyze the distribution of three stress proteins (HSP25,
HSP72, GRP75) in rat kidney and liver orally exposed to Al sulphate daily for 3 and 6 months. A1-induced
alterations were further studied by histopathology (H&E, PAS, Perl's, Masson) and ultrastructural
morphometry. In the kidney: HSP25 was enhanced in proximal tubules after 6 months A1-exposure when
abnormal brush borders were observed; HSP72 was induced in proximal tubules only after long Al-treatment;
GRP75 was raised in midcortical area sometimes within nuclei. Furthermore, lysosomal and lipofuscins
densities increased in the juxtamedullary tubules after 3 months Al exposure with respect to controls. In the
liver: Perl'spositive deposits and fibrosis became evident after Al treatment. HSP25 was very weak; HSP72
focal in pericentral hepatocytes at 3 months and induced also in Kupffer cells at 6 months; GRP75 diffuse in
periportal hepatocytes and non parenchymal cells at 6 months. Prolonged Al exposure stimulated stress
proteins strictly organ-dependently in the rat. Their distribution in kidney and liver seems related to cumulative
sublethal effects induced by metal and could be a sensitive index of Al susceptibility of these organs.
Pag. 1 di 300 31/08/2011 ID nr. 3229
Paton DJ, De_Clercq K, Greiner M, Dekker A, Brocchi° E, Bergmann I, Sammin JD,
Gubbins S, Parida S
Application of non-structural protein antibody tests in substantiating freedom from foot-and-mouth
disease virus infection after emergency vaccination of cattle
Vaccine. - Vol. 24 no 42-43 ( 2006). - p 6503-6512. - 20 bib ref
Impact factor: 2,822
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
There has been rnuch debate about the use of the so-called "vaccinate-to-live policy for the control of footand-mouth disease (FMD) in Europe. according to which, spread of the FMD virus (FMDV) from future
outbreaks could be controlled by a short period of "emergency)," vaccination of surrounding herds, reducing
the need for large-scale pre-emptive culling of at-risk animals. Since vaccinated animals may become
subclinically infected with FA9DV following challenge exposure, it is necessary tO either remove all vaccinates
(vaccinate-to-kill) or to detect and remove vaccinates in which virus is circulating or has established persistent
infections (vaccinate-to-live), in order to rapidly regain the most favoured trading status of FMD-free without
vaccination. The latter approach can be supported by testing vaccinated animals for the present of antibodies
to certain non-structural proteins NSP) of FMDV, which are induced by infection with the virus, but not by
vaccination wíth purified FMD vaccines. Using test sensitivity and specificity data established at a recent
workshop on NSP assays [Brocchi E. Bergmann I. Dekker A. Paton DJ. Sammin DJ. Greiner M. et al.
Comparative performance of six ELISAs for antibodies to the non-structural proteins of foot-and-mouth
disease. Vaccine. in press, this paper examines the ways in which serological testing with NSP ELISAs can
he used and interpreted and the effect that this will have un the confidence with which freedom from infection
can be demonstrated within guidelines specified by the World Animal Health Organisation and the European
Commission.
ID nr. 3250
Pedretti° E, Passeri B, Amadori° M, Isola P, Di_Pede P, Telera A, Vescovini R, Quintavalla
F, Pistello M
Low-dose interferon-alfa treatment for feline immunodeficiency virus infection
Vet Immunol Immunopathol. - Vol. 109 ( 2006). - p 245-254. - 31 bib ref
Impact factor: 1,626
Feline immunodeficiency virus sustains an AIDS-like syndrome in cats, which is considered a relevant model
for human AIDS. Under precise enrolment requirements, 30 naturally infected cats showing overt disease
were included in a trial of lowdose, oral human interferon-a treatment. Twenty-four of them received 10 IU/Kg
of human interferon-alfa and 6 placebo only on a daily basis under veterinary supervision. The low-dose
human interferon-alfa treatment significantly prolonged the survival of virus-infected cats (p < 0.01) and
brought to a rapid improvement of disease conditions in the infected hosts. Amelioration of clinical conditions
was neither correlated with plasma viremia, nor with proviral load in leukocytes. A good survival of CD4+T
cells and a slow increase of CD8+T cells were also observed in human interferon-alfa-treated cats.
Interestingly, the improvement of the total leukocyte counts showed a much stronger correlation with the
recovery from serious opportunistic infections. As shown in other models of low-dose interferon-alfa treatment,
there was a rapid regression of overt immunopathological conditions in virus-infected cats. This hints at a
major role of interferon-a in the control circuits of inflammatory cytokines, which was probably the very
foundation of the improved clinical.score and survival despite the unabated persistence of virus and
Pag. 2 di 300 31/08/2011 virusinfected cells.
ID nr. 3268
Nunez JI, Fusi° P, Borrego° B, Brocchi° E, Pacciarini° ML, Sobrino F
Genomic and antigenic characterization of viruses from the 1993 Italian foot-and-mouth disease
outbreak
Arch Virol. - Vol. 151 ( 2006). - p 127-142. - 57 bib ref
Impact factor: 1,819
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
PRC98004 - Update of reagents and procedures for diagnosis and control of Foot-and-mouth
disease toward recognition of newly arrived viral strains - Brocchi Dr.ssa Emiliana - Solo - MINSAN
- 1998 - 01/10/1999 0.00.00 - 30/09/2001 0.00.00 - 190000000,00 LIT 1 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
The origin and evolution of the type O foot-and-mouth disease viruses (FMDV) that caused the outbreak
occurrence in Italy in 1993, the first episode of the disease in the EU after adoption of a non-vaccination policy
in 1991, have been studied by the analysis of sequences encoding three main antigenic sites on the viral
capsid proteins. The phylogenetic tree derived from sequences spanning the carboxyterminal end of VP 1
showed that these Italian viruses were grouped in the ME-SA topotype, closely related to viruses that
circulated previously in the Middle East. The analysis of the nucleotide sequences in VP1, VP2 and VP3
showed a co-circulation during the epizootic of genetic variants, including viruses with amino acid
replacements in VP3. For some of the isolates analyzed, values of fixation of nucleotide substitutions per year
were observed in the three regions analyzed, ranging from 1.5 to 5.1 x 10-2. The use of a panel of new
monoclonal antibodies raised against an isolate from this outbreak, as well as monoclonal antibodies to FMDV
01-Switzerland 1965, showed differences in the reactivity pattern among some of the Italian isolates analyzed,
which were consistent with the co-circulation of antigenic variants. These results support the potential for
FMDV diversification in a limited period of time and under epidemiological conditions in which no vaccination
campaigns were being implemented.
ID nr. 3285
Gelmetti° D, Meroni° A, Brocchi° E, Koenen F, Cammarata G
Pathogenesis of encephalomyocarditis experimental infection in young piglets: a potential animal
model to study viral myocarditis
Vet Res. - Vol. 37 ( 2006). - p 15-23. - 41 bib ref
Impact factor: 2,324
Project PL 98 - 4146 - Molecular characterisation and epidemiology of Encephalomyocarditis virus: a model
for emerging diseases - Koenen Dr. F. - UO - CE - 1998 - 1252600,00 EUR - 01/02/1999 0.00.00
- 31/01/2002 0.00.00 - 3 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
The pathogenesis of encephalomyocarditis (ENIC) due to the ENIC virus (EMCV) was studied in 24 piglets
oro-nasally infected with the field isolate B279/95. Two pigs were kept as negative controls and were
euthanised at hour 0. The remaining 24 were euthanised every 6 h up to 78-h post infection (hpi). Virus
isolation, histological examination and EMCV immunodetection were performed on the spleen, intestine,
pancreas, liver, kidneys, heart, lungs, lymph nodes, tonsils and brain. EMCV was isolated at 6-hpl from the
intestine and lymph nodes and at 12-hpi from the heart. From 6 to 12-hpi, scattered degenerate
myocardiocytes were immunolabelled. Subsequently, myocarditis developed and progressively worsened.
Immunopositive reaction in tonsil macrophages, observed in the early stage of infection (6-hpi), suggests that
Pag. 3 di 300 31/08/2011 tonsils are the portal of entry, and by mean of wandering macrophages the ENIC virus is then distributed
through the body. Afterwards, EMCV-B279/95 replicates intensively in the cytoplasm of myocardiocytes and
the acute myocarditis is strictly related to the tropism of these cells. Four pigs died spontaneously. In three
animals no post mortem lesions or virus were isolated/detected, although all of them showed mild myocarditis.
The experimental infection with EMCV B279,95 indicates: (i) the experimental protocol mimics the individual
variability observed in natural disease, (ii) tonsils are the portal of entry of infection and the heart is the target
organ, (iii) EMCV provides a valuable animal model for comparative studies on progressive viral myocarditis.
ID nr. 3307
Bolelli L, Bobrovovà Z, Ferri E, Fini F, Menotta° S, Scandurra° S, Fedrizzi° G, Girotti S
Bioluminescent bacteria assay of veterinary drugs in excreta of food-producing animals
J Pharm Biomed Anal. - Vol. 42 ( 2006). - p 88-93. - 27 bib ref
Impact factor: 1,889
The residues of pharmacological treatments on food-producing animals, present in the manure dispersed on
agricultural land, can impact environmental and human health through toxic, genotoxic, and drug-resistance
development effects. Biotoxicity assays can easily reveal the presence of noxious substances and those
based on bioluminescent bacteria (BLB) are particularly simple and rapid. A BLB assay was developed as
microplate format by using various strains of Vibrio sp. and was employed to evaluate their response to pure
antibiotic solutions and to residues extracted from excreta of antibiotic treated pigs and turkeys. The residues
were quantified by HPLC analysis. The BLB assay can be proposed as an easy-to-perform screening tool to
assess the presence of residues due to undeclared current, or recently ended, pharmacological treatments,
as well as to evaluate their permanence in manure.
ID nr. 3308
Petracci M, Bianchi M, Cavani C, Gaspari P, Lavazza° A
Preslaughter mortality in broiler chickens, turkeys, and spent hens under commercial slaughtering
Poult Sci. - Vol. 85 no 9 ( 2006). - p 1160-1164. - 25 bib ref
Impact factor: 1,747
The incidence of dead on arrival (DOA) birds was surveyed over 33 broiler, 11 turkey, and 19 spent hen
abattoirs representing the majority (around 70%) of the Italian poultry slaughter plants. Data were recorded
monthly during a 4-yr period (August 2001 to July 2005), considering a total of 1,266 million chicken broilers,
118 million turkeys, and 54 million spent hens, which represent 67.7, 84.0, and 28.4% of the national
production, respectively. The overall average incidence of DOA was found to be 0.35, 0.38, and 1.22% in
broilers, turkeys, and spent hens, respectively. The season significantly (P S 0.01) influenced the mortality of
all considered poultry categories, with higher incidence being observed during the summer (0.47, 0.52, and
1.62% for broilers, turkeys, and spent layers, respectively). The incidence of DOA broilers was found to be
lower in small slaughter plants compared with medium and large slaughter plants (0.28 vs. 0.38 and 0.35%, P
<_ 0.01). The data obtained in this study might be used for establishing limit values of DOA as a welfare
indicator during the preslaughter time of birds, including catching, loading, transportation, and lairage.
ID nr. 3316
Alborali° L
Climatic variations related to fish diseases and production
Vet Res Commun. - Vol. 30 suppl 1 ( 2006). - p 93-97. - 27 bib ref
Impact factor: 0,328
First author Alborali, Giovanni Loris
Pag. 4 di 300 31/08/2011 ID nr. 3317
Petrini° S, Ferrari° M, Vincenzetti S, Vita A, Amici A, Ramadori G
An immunoenzyme linked assay (ELISA) for the detection of antibodies to Truncated Glycoprotein D
(tgD) of bovine Herpesvirus-1
Vet Res Commun. - Vol. 30 suppl 1 ( 2006). - p 257-259. - 8 bib ref
Impact factor: 0,328
Project PRC2000004 - New strategies for control of Influenza virus infection in swine and horses. Barigazzi Dr. Giuseppe - Capofila - MINSAN - 2000 - 670000000,00 LIT - 02/05/2002 0.00.00 01/08/2005 0.00.00 - 1 - N5 - Parma - Foni Dr.ssa Emanuela - I
2 - N1 - Bologna - Vecchi Dr. Giovanni - I
3 - ISS - Donatelli Dr.ssa Isabella - E
4 - ISS 2 - Campitelli Dr.ssa Laura - E
5 - L1 - Centro Substrati Cellulari - Ferrari Dr.ssa Maura - I
ID nr. 3346
Ferris NP, King DP, Reid SM, Hutchings GH, Shaw AE, Paton DJ, Goris N, Haas B,
Hoffmann B, Brocchi° E, Bugnetti° M, Dekker A, De Clercq K
Foot-and-mouth disease virus : a first inter-laboratory comparison trial to evaluate virus isolation
and RT-PCR detection methods
Vet Microbiol. - Vol. 117 ( 2006). - p 130-140. - 27 bib ref
Impact factor: 2,175
Project 2003-SSP-3-513645 - New and emerging technologies: improved laboratory and on-site detection of
oie list a viruses in animals and animal products (LAB-ON.SITE) - Belak Dr. Sandor - UO - CE 2004 - 1500000,00 EUR - 01/11/2004 0.00.00 - 31/07/2008 0.00.00 - 7 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
PRC99004 - Development and validation of innovating serological methods for the differentiation of
vaccinated from FMD-virus infected animals based on the use of multiple non-structural antigens Brocchi Dr.ssa Emiliana - Solo - MINSAN - 1999 - 16/08/2000 0.00.00 - 15/08/2002 0.00.00 142524000,00 LIT 1 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
Five European reference laboratories participated in an exercise to evaluate the sensitivity and specificity of
their routinely employed RT-PCR tests and cell cultures for the detection and isolation of foot-and-mouth
disease (Fk1D) virus. Five identical sets of 20 coded samples were prepared from 10 vesicular epithelia,
which were derived from submissions from suspect cases of FNID or swine vesicular disease (SVD). Sixteen
samples were derived from six FMD virus positive epithelia representing four different serotypes (two each of
types O and A and one each of types Asia 1 and SAT 2), two from samples which had been found to be
negative by antigen ELISA and virus isolation (VI) in cell culture and two from SVD virus positive epithelia.
Some of the FNID virus positive samples were prepared from 10-fold serial dilutions of three of the initial
suspensions. Each laboratory tested the samples by one or more of its available RT-PCR procedures and
inoculated cell cultures that it routinely uses for FMD diagnosis in attempts to isolate virus, the specificity of
which was confirmed by antigen ELISA. The best of the RT-PCR assays used in each laboratory gave
comparable results while the sensitivity of cell cultures was variable from high in one laboratory. moderate in
two and low in two others. This prototype panel of samples would appear suitable for external quality
assurance of these tests but would benefit from the inclusion of more negative samples and an extension in
the serial dilution range of one or more of the FMD positive sample titration series.
ID nr. 3380
Stacchiotti A, Ricci F, Rezzani R, Li_Volti G, Borsani E, Lavazza° A, Bianchi R, Rodella
LF
Tubular stress proteins and nitric oxide synthase expression in rat kidney exposed to mercuric
Pag. 5 di 300 31/08/2011 chioride and melatonin
J Histochem Cytochem. - Vol. 54 no 10 ( 2006). - p 1149-1157. - 46 bib ref
Impact factor: 2,208
Stress proteins such as HSP70 members (HSP72 and GRP75) and metallothionein (MT) protect the Kidney
against oxidative damage and harmful metals, whereas inducible nitric oxide synthase (iNOS) regulates
tubular functions. A single dose of mercuric chloride (HgCI2) can cause acute renal failure in rats, its main
target being the proximal tubule. Oxidative damage has been proposed as one of its pathogenic mechanisms.
In this study we tested whether melatonin (MEL), a powerful antioxidant compound, is effective against
HgCI2 nephrotoxicity. Rats were treated with saline, HgCI2 (3.5 mg/kg), MEL (5 mg/I<9), and MEL + HgCl2
and examined after 24 hr for HSP72, GRP75, MT, and iNOS by immunohistochemistry and immunoblotting.
Tubular effects of the treatment were then characterized by ultrastructure. In the HgCI2 group, all markers
were overexpressed in convoluted proximal tubules and sometimes in distal tubules. In the MEL + HgCI2
group, GRP75 and iNOS decreased in convoluted and straight proximal tubules, whereas HSP72 and MT
persisted more than the saline and MEL-only groups. Tubular damage and mitochondrial morphometry were
improved by MEL pretreatment. In conclusion, the beneficial effect of MEL against nephrotoxicity HgCI2
nephrotoxicity was outlined morphologically and by the reduction of the tubular melatonin expression of stress
proteins and iNOS. These markers could represent sensitive recovery stress proteins index against mercury.
ID nr. 3402
Scala A, Garippa G, Varcasia A,Tranquillo° VM, Genchi C
Cystic echinococcosis in slaughtered sheep in Sardinia (Italy)
Vet Parasitol. - Vol. 135 nr. 1 ( 2006). - p 33-38 - 28 bib ref
Impact factor: 1,686
Of 771 regularly slaughtered Sardinian breed sheep, 580 (75%) were found infected with Echinococcus
granulosus hydatid cysts. Seventy-nine sheep (10.3%) had at least 1 fertile cyst. The prevalence of sheep
infected with purulent/caseous cysts, calcified cysts and sterile cysts was 13, 59 and 28%, respectively. The
age of sheep was positively associated with the probability of infection that increased 1.15 fold for each further
year of age. Fertile cysts were found in the lungs of 46 sheep (6%) and in the liver of 13 sheep (1.7%), and in
the lung and the liver of 20 sheep (2.6%). Most fertile cysts were found in the lungs (314) and most sheep
were infected with less than 10 cysts. When analyzed by a mixed-effect logistic model, the probability to find
fertile cysts in the lungs was three times higher compared to the liver and it increased with the age of the
sheep (p = 0.70, p < 0.001). Of 4072 collected cysts, 532 were fertile, 178 purulent/caseous. 2 339 calcified
and 1023 sterile.
ID nr. 3414
Furlanello T, Tasca S, Caldin M, Carli E, Patron C, Tranquillo° M, Lubas G,
Solano_Gallego L
Artifactual changes in canine blood following storage, detected using the ADVIA 120 hematology
analyzer
Vet Clin Pathol. - Vol. 35 no 1 ( 2006). - p 42-46 - 20 bib ref
Impact factor: 0,795
Artifactual changes in blood may occur as a consequence of delayed analysis and may complicate
interpretation of CBC data. Objective: The aim of this study was to characterize artifactual changes in canine
blood, due to storage, using the ADVIA 120 hematology analyzer. Methods: Blood samples were collected
into EDTA from 5 clinically healthy dogs. Within 1 hour alter blood sample collection and at 12, 24, 36 and 48
hours alter storage of the samples at either 4'C or room temperature (-24'C), a CBC was clone using the
ADVIA 120 and multispecies software. A linear mixed model was used to statistically evaluate significant
differences in values over time, compared with initial values. Results: The HCT and MCV were increased
Pag. 6 di 300 31/08/2011 significantly after 12 hours of collection at both 4'C and 24'C, and continued to increase through 48 hours. The
MCHC initially decreased significantly at 12-24 hours and then continued to decrease through 48 hours at
both temperatures. Changes in HCT, MCV, and MCHC were greater at 24'C than at 4'C at all time points. A
significant increase in MPV and a decrease in mean platelet component concentration were observed at all
time points at 24'C. Samples stored at 24'C for 48 hours had significantly higher percentages of normocytichypochromic RBCs, and macrocytic-normochromic RBCs, and lower platelet and total WBC counts.
Conclusions: Delayed analysis of canine blood samples produces artifactual changes in CBC results, mainly
in RBC morphology and platelet parameters, that are readily detected using the ADVIA 120. Refrigeration of
specimen, even after 24 hours of storage at room temperature, is recommended to improve the accuracy of
CBC results for canine blood samples.
ID nr. 3424
Furianello T, Caldin M, Stocco A, Tudone E, Tranquillo° M, Lubas G, Solano-Gallego L
Stability of stored canine plasma for hemostasis testing
Vet Clin Pathol. - Vol. 35 no 2 ( 2006). - p 204-207 - 14 bib ref
Impact factor: 0,795
ID nr. 3425
De_Lorenzi D, Mandara MT, Tranquillo° M, Baroni M, Gasparinetti N, Gandini G,
Masserdotti C, Bonfanti U, Bertolini G, Vian P, Bernardini M
Squash–prep cytology in the diagnosis of canine and feline nervous system lesions: a study of 42
cases
Vet Clin Pathol. - Vol. 35 no 2 ( 2006). - p 208-214 - 40 bib ref
Impact factor: 0,795
ID nr. 3426
Croci L, Losio° MN, Suffredini E, Pavoni° E, Di_Pasquale E, Fallacara° F, Arcangeli G
Assessment of human enteric viruses in shellfish from the northern Adriatic sea
Int J Food Microbiol. - Vol. 114 no 2 ( 2007). - p 252-257. - 41 bib ref
Impact factor: 2,608
Incidence and circulation of different strains of hepatitis A and Norovirus in shellfish were studied on 235
samples (Tapes philippinarum. mytilus galloprovincialis. Ostrea spp. and Chlamys spp.) obtained from
different sites, representing the shellfish production areas of the northem Adriatic sea. Shellfish were
harvested in the period of one year and, after depuration, were examined for bacterial (Escherichia coli and
Salmonella) and viral (HAV and NoV) contamination. Viral contamination was present on average in 22% of
samples: specifically, 6% of samples tested positive for HAV. 14% for NoV and 2% for both viruses. None of
the samples revealed the presence of Salmonella, and in most of them (93%) the number of E. coli was below
the European legislation limit of 230 MPN/100 g. T. philippinarum was the species most often contaminated,
as well as bein the only species in which the legal limit for E. coli was, in some cases, exceeded. Both HAV
and NoV contamination were detected throughout the year; NoV detection was slightly more frequent during
winter months, but positive samples were also present in summer. The sequencing of the PCR products
showed the circulation of only one HAV genotype (LA) and four different NoV genotypes (Hawaii, Melksham.
Lordsdale and GGIIb) with a prevalence of the GGIIb genotype in the sceond period of the monitoring.
ID nr. 3432
Ostanello F, Dottori° M, Gusmara G, Leotti G, Sala V
Pneumonia disease assessment using a slaughterhouse lung-scoring method
Pag. 7 di 300 31/08/2011 J Vet Med A. - Vol. 54 no 2 ( 2007). - p 70-75. - 33 bib ref
Impact factor: 0,627
The aims of this study were the evaluation of a quantitative method for the assessment of pneumonia lesions
applied lo heavy-weight slaughtered pies. the identification of risk factors connected with the increase in the
prevalence and severity of the lesions and the evaluation of a possible correlation between the presence of
pneumonia lesions and the decrease in the carcass quality. The lungs of 10 041 pigs (109 slaughtered
batches) coming from 91 farms located in Northern Italy were examined. Lung lesions were scored using the
method developed by Madec and Kobisch (Juurn. Rech. Porc. Fr.. 14. 1992. 405). Before the scoring.
anamnestic information regarding the farm of origin of each batch were collected. For 41 batches (3603 pigs).
information about carcass quality were also collected. Pneumonia lesions were found in 59.6°'0 of the lungs
(range 3-91%), and the average batch score was 2.11 (range 0.03-7.15). We identified as farm risk factors
those related to an increase in the severity of the lung lesions. the presence of breeders within the herd. the
starting of a growing cycle during the winter season and the lack of vaccination programmes to Mycoplasma
hyopneumoniae. Moreover, we also found a statistically significant association between the increase in the
mean lung score of the batch and the decrease of the carcass quality.
ID nr. 3433
Capobianco R, Casalone C, Suardi S, Mangieri M, Miccolo C, Limido L, Catania M, Rossi
G, Di_Fede G, Giaccone G, Bruzzone MG, Minati L, Corona C, Acutis P, Gelmetti° D,
Lombardi° G, Groschup MH, Buschmann A, Zanusso G, Monaco S, Caramelli M,
Tagliavini
Conversion of the base prion strain into the BSE strain: the origin of BSE?
PLoS Pathog. - Vol. 3 no 3 ( 2007). - p 1-7. - 38 bib ref
Impact factor: 6,056
Project PRC2004008 - Preliminary assessment of the presence of hepatitis E infection in swine of
Lombardy, set-up of an ELISA serological method and PCR - Lombardi Dr. Guerino - Solo MINSAN - 2004 - 50000,00 EUR - 01/07/2005 0.00.00 - 30/06/2007 0.00.00 - 1 - G3 - Reparto
benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Lombardi Dr. Guerino - I
Atypical neuropathological and molecular phenotypes of bovine spongiform encephalopathy (BSE) have
recently been identified in different countries. One of these phenotypes, named bovine "amyloidotic"
spongiform encephalopathy (BASE), differs from classical BSE for the occurrence of a distinct type of the
disease-associated prion protein (PrP), termed PrPSc, and the presence of PrP amyloid plaques. Here, we
show that the agents responsible for BSE and BASE possess different biological properties upon transmission
to transgenic mice expressing bovine PrP and inbred lines of nontransgenic mice. Strikingly, serial passages
of the BASE strain to nontransgenic mice induted a neuropathological and molecular disease phenotype
indistinguishable from that of BSE-infected mice. The existence of more than one agent associated with prion
disease in cattle and the ability of the BASE strain to convert into the BSE strain may have important
implications with respect to the origin of BSE and spongiform encephalopathies in other species, including
humans.
ID nr. 3435
Amadori° M
The role of IFN-a as homeostatic agent in the inflammatory response: a balance between danger and
response?
J Interferon Cytokine Res. - Vol. 27 ( 2007). - p 181-189. - 79 bib ref
Impact factor: 2,472
Project PRC2005002 - Assessment and modulation of the inflammatory effects of early weaning in the piglet
- Amadori Dr. Massimo - Solo - MINSAN - 2005 - 22000,00 EUR - 15/05/2006 0.00.00 -
Pag. 8 di 300 31/08/2011 14/11/2008 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
Interferon-a (IFN- a) is thought of by most immunologists as a fundamental component of the antiviral innate
immune response, with other, accessory properties. Several lines of evidence point to a fundamental
reappraisal of this conceptual framework because it may neglect other crucial functions of this cytokine under
both health and disease conditions. Among these, a regulatory role in the inflammatory response is probably
of paramount importance, as shown by in vivo and in vitro studies on humans, pets, and farm and laboratory
animals. This role would not conflict with the main functions of IFN-a in the innate immune system and would
complement these in line with major, evolution-based needs of the host. A hinge role of IFN-a between innate
and adaptive immunity was recognized in the past on the basis of compelling evidence. This concept should
now be widened; not only does IFN-a act to trigger, amplify, and sustain the different phases of the immune
response, but it could also promote a substantial balance between danger and inflammatory response when
an infectious challenge is either declining or completely over.
ID nr. 3439
Duan L, Campitelli L, Fan HX, Leung, YHC, Vijaykrishna D, Zhang JX, Donatelli I, Delogu
M, Li KS, Foni° E, Chiapponi° C, Wu WL, Kai H, Webster RG, Shortridge KF, Peiris JSM,
Smith GJD, Chen H, Guan Y
Characterization of low-pathogenic H5 subtype influenza viruses from Eurasia: implications for the
origin of highly pathogenic H5N1 viruses
J Virol. - Vol. 81 no 14 ( 2007). - p 7529-7539. - 50 bib ref
Impact factor: 5,341
Highly pathogenic avian influenza (HPAI) HSNI viruses are now endemiec in many Asian countries, resulting
in repeated outbreaks in poultry and increased cases of human infection. The immediate precursor of these
HPAI viruses is believed to be Algoose/Guangdong/1/96 (Gs/GD)-like H5NI HPAI viruses first detected in
Guangdong, China, in 1996. From 2000 onwards, many novel reassortant H5N1 influenza viruses or
genotypes have emerged in southern China. However, precursore of the Gs/GD-like viruses and their
subsequent reassortants have not been fully determined. Here we characterize low-pathogenic avian
influenza (LPAI) HS subtype viruses isolated from poultry and migratory birds in southern China and Europe
from the 1970s to the 2000s. Phylogenetíc analyses revealed that Gs/GD-like virus was likely derived from an
LPAI H5 virus in migratory birds. However, its variants arose from multiple reassortments between Gs/GD-like
virus and viruses from migratory birds or with those Eurasian viruses isolated in the 1970s. It is of note that
unlíke HPAI H5N1 viruses, those recent LPAI H5 viruses have not become established in aquatie or terrestrial
poultry. Phylogenetic analyses revealed the dynamic nature of the influenza virus gene pool in Eurasia with
repeated transmissions between the eastern and western extremities of the continent. The data allo show
reassortment between influenza viruses from domestie and migratory birds in thìs region that has contributed
to the expanded diversity of the influenza virus gene pool among poultry in Eurasia.
ID nr. 3493
Gasparini° M, Assini° W, Bozzoni° E, Tognoli° N, Dusi° G
Development and validation of a liquid chromatography–tandem mass spectrometry method for the
separation of conjugated and unconjugated 17(alfa).- and 17 ß-boldenone in urine sample
Anal Chim Acta. - Vol. 586 ( 2007). - p 154-162. - 18 bib ref
Impact factor: 2,894
Natural occurrence or illegal treatment of boldenone (BOLD) presence in cattle urine is under debate within
the European Union. Separation of conjugated and unconjugated forms of 17a-boldenone (a-BOLD) and
17(3-boldenone ((3-BOLD) and presence of related molecules as androsta-1,4-diene-3,17-dione (ADD)
appear critical points for the decision of an illegal use. The aim of this study is a new analytical approach of
Pag. 9 di 300 31/08/2011 BOLD and ADD confirmation in cattle urine. The separation between conjugated and unconjugated forms of
BOLD was obtained by a preliminary urine liquid–liquid extraction step with ethyl acetate. In this step the
organic phase extracts only unconjugated BOLD and ADD, while BOLD in conjugated form remain in urine
phase.. Afterwards the urine phase, contains conjugated BOLD, was suhjected to an enzymatic
deconjugation. Solid-phase extraction (OASIS-HLB Waters) was used for the purification and concentration of
analytes in organic and urine phases and liquid chromatography ion electrospray tandem mass spectrometry
(LC–MS–MS) was applied for the confirmation of BOLD and ADD, using deuteriumlabelled 17(3-boldenone
(BOLD-d3) as internal standard. The method was validated as a quantitative confirmatory method according
to the Commission Decision 2002/657/CE. The results obtained demonstrate that the developed method show
very high specificity, pecision, trueness and ruggedness. Decision limits (CCoc) smaller than 0.5 ng mL-' were
obtained for each analyte.
ID nr. 3499
Di_Francesco A, Baldelli R, Cevenini R, Magnino° S, Pignanelli S, Salvatore D, Galuppi R,
Donati M
Seroprevalence to chlamydiae in pigs in Italy
Vet Rec. - Vol. 159 no 25 ( 2006). - p 849-850. - 23 bib ref
Impact factor: 1,017
ID nr. 3517
Lanzi° G, de_Miranda JR, Boniotti° MB, Cameron GE, Lavazza° A, Capucci° L, Camazine
SM, Rossi C
Molecular and biological characterization of deformed wing virus of honeybees (Apis mellifera L.)
J Virol. - Vol. 80 no 10 ( 2006). - p 4998-5009. - 77 bib ref
Impact factor: 5,178
Project AMA - An investigation into Varroa-associated viruses of bees. - Sabatini Dr.ssa Anna Gloria - UO
- MIPA - 1999 - 0,00 LIT - 06/12/2000 0.00.00 - 07/01/1976 0.00.00 - 1 - H2 - Reparto di
Biologia Molecolare - Rossi Dr. Cesare - I
Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing
deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified
from diseased insects, and its genome was cloned and sequenced. The genomic RNA of DWV is 10,140
nucleotides in length and contains a single large open reading frame encoding a 328-kDa polyprotein. The
coding sequence is flanked by a 1,144-nucleotide 5' nontranslated leader sequence and a 317-nucleotide 3'
nontranslated region, followed by a poly(A) tail. The three major structural proteins, VP1 (44 kDa), VP2 (32
kDa), and VP3 (28 kDa), were identified, and their genes were mapped to the N-terminal section of the
polyprotein. The C-terminal part of the polyprotein contains sequence motifs typical of well-characterized
picornavirus nonstructural proteins: an RNA helicase, a chymotrypsin-like 3C protease, and an RNAdependent RNA polymerase. The genome organization, capsid morphology, and sequence comparison data
indicate that DWV is a member of the recently established genus I flavirus.
ID nr. 3518
Stacchiotti A, Borsani E, Riccia F, Lavazza° A, Rezzani R, Bianchi R, Rodella LF
Bimoclomol ameliorates mercuric chloride nephrotoxicity through recruitment of stress proteins
Toxicol Lett. - Vol. 166 ( 2006). - p 168-177. - 42 bib ref
Impact factor: 2,43
Bimoclomol (BIM), is a stress proteins coinducer, that acts synergistically with a mild stressor to activate
cytoprotective stress proteins. BIM has been successfully utilized in animal models for the treatment of
Pag. 10 di 300 31/08/2011 various nervous, cardiac and cerebrovascular diseases. Mercuric chloride (HgC12) induces acute renal failure
in rats by a single dosage. The present in vivo study was conduc to assess the efficacy of BIM against acute
HgC12 nephrotoxicity. At different times after BIM and/or HgC12 exposure we evaluated renal morphology
and the localization/abundance of three stress proteins (HSP72, GRP75, HSP60) by electron microscopy
immunohistochemistry and Western blot analysis. BIM delivery to rats 6 h before mercury, ameliorated
damage to renal ultrastructure, with recovery of tubular and mitochondrial membranes 24 h after mercury
treatment. In rats pretreated with BIM prior HgC12 exposure, HSP72 was significantly overexpressed in
proximal tubules in a time-dependent manner. In contrast, the amou of GRP75 and HSP60 after BIM
pretreatment were comparable to the group treated with mercury alone, but these stress proteins had
translocated to the nuclei at 14 and 24 h, respectively. These novel findings suggest that BIM mitigates H902
nephrotoxicity in rats through the early recruitment of stress proteina midcortical proximal tubules that are the
main renal mercury-targets.
ID nr. 3519
Martella V, Bànyai K, Ciarlet M, Iturriza_Gómara M, Lorusso E, De_Grazia S, Arista S,
Decaro N, Elia G, Cavalli A, Corrente M, Lavazza° A, Baselga R, Buonavoglia C
Relationships among porcine and human P[6] rotaviruses : evidence that the different human P[6]
lineages have originated from multiple interspecies transmission events
Virology. - Vol. 344 ( 2006). - p 509-519. - 64 bib ref
Impact factor: 3,08
Project PRF2003307 - Genetic and antigenic diversity of rotaviruses – study of the evolutionary mechanisms
and implications for diagnosis and vaccination - Gentile Dr. Mattia - UO - MINSAN - 2003 256800,00 EUR - 01/03/2004 0.00.00 - 31/10/2006 0.00.00 - 7 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
Porcine rotavirus strains (PoRVs) bearing human-like VP4 P[6] gene alleles were identified. Genetic
characterization with either PCR genotyping or sequence analysis allowed to determine the VP7 specificity of
the PoRVs as G3, G4, G5 and G9, and the VP6 as genogroup I, that is predictive of a subgroup I specificity.
Sequence analysis of the VP8* trypsin-cleavage product of VP4 allowed PoRVs to be characterized further
into genetic lineages within the P[6] genotype. Unexpectedly, the strains displayed significantly higher
similarity (up to 94.6% and 92.5% at aa and nt level, respectively) to human M37-like P[6] strains (lineage 1),
serologically classifiable as P2A, or to the atypical Hungarian P[6] human strains (HRVs), designated as
lineage V (up to 97.0% aa and 96.1% nt), than to the porcine P[6] strain Gottfried, lineage II «85.1% aa and
82.2 nt), which is serologically classified as P213. Interestingly, no P[6] PoRV resembling the original
prototype porcine strain, Gottfried, was detected, while Japanase P[6] PoRV clustered with the atypical
Japanasc GI human strain AU 19. By analysis of the l Oth and 11th genome segments, all the strains
revealed a NSP413 genogroup (Wa-like) and a NSP5/6 gene of porcine origin. These findings strongly
suggest interspecies transmission of rotavirus strains and/or genes, and may indicate the occurrence of at
least 3 separate rotavirus transmission events between pigs and human, providing convincing evidence that
evolution of human rotaviruses is tightly intermingled with the evolution of animal rotaviruses.
ID nr. 3520
Martella V, Ciarlet M, Bànyai K, Lorusso E, Cavalli A, Corrente M, Elia G, Arista S,
Camero M, Desario C, Decaro N, Lavazza° A, Buonavoglia C
Identification of a novel VP4 genotype carried by a serotype G5 porcine rotavirus strain
Virology. - Vol. 346 ( 2006). - p 301-311. - 73 bib ref
Impact factor: 3,08
Project PRF2003307 - Genetic and antigenic diversity of rotaviruses – study of the evolutionary mechanisms
and implications for diagnosis and vaccination - Gentile Dr. Mattia - UO - MINSAN - 2003 256800,00 EUR - 01/03/2004 0.00.00 - 31/10/2006 0.00.00 - 7 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
Pag. 11 di 300 31/08/2011 Rotavitus genome segment 4, encoding the spike outer capsid VP4 protein, of a porcine rotavirus (PoRV)
strain, 134.04-15, identified in Italy was sequenced, and the predicted amino acid (aa) sequence was
compared to those of all known VP4 (P) genotypes. The aa sequence of the fullength VP4 protein of the
PoRV strain 134.'04-15 showed aa identity values ranging from 59.7% (bovine strain KK3, P8[11]) to 86.09%
(porcine strain A46, P[13]) with those of the remaining 25 P genotypes. Moreover, aa sequence analysis of
the corresponding VP8* trypsin cleavage fragment revealed that the PoRV strain 134/ 4-15 shared low
identity, ranging from 37.52'% (bovine strain 993'83, P[t 7]) to 73.6°% (porcine strain MDR-13, P[13]), with
those of the remaining 25 P genotypes. Phylogenetic relationships showed that the VP4 of the PoRV strain
134.;04-15 shares a common evolutionary origin with porcine P[13] and tapine P[22] rotavirus strains.
Additional sequence analyses of the VP7, VP6, and NSP4 genes of the PoRV strain 134/04-15 revealed the
highest VP7 aa identity (95.9%r) to G5 porcine strains, a porcine-like VP6 within VP6 genogroup I, and a Walike (genotype B) NSP4, respectively. Altogether, these results indicate that the PoRV strain 134/04-15 should
be considered as prototype of a new VP4 genotype, P[26], and provide further evidence for the vast genetic
and antigenic diversity of group A rotavinises.
ID nr. 3536
Ferrari M
Differentiative potential of cardiomyocyte satellite cells and possible utilization in cardiac tissue
repair
Eur J Histochemistry. - Vol. 3 ( 2006). - p 210?
Workshop "Skeletal Muscle Fiber: Morpho-Functional Patterns and Technical Approaches " : Urbino, Italy :
October 3-6th, 2006)
Impact factor: 0,99
Project PRF2003304 - Comparative study of the efficacy of the immunizing systems towards type 1 herpetic
viruses in humans and animals - Guercio Dr.ssa Annalisa - UO - MINSAN - 2003 - 212500,00
EUR - 01/02/2004 0.00.00 - 30/09/2006 0.00.00 - 1 - L1 - Centro Substrati Cellulari - Ferrari
Dr.ssa Maura - I
One possible strategy in the utilization of cell precursors in cardiac tissue damage repair concerns satellite
cells. These are localised between mature myofiber basal lamina and cell membrane, on fiber surface. There
are responsible of muscle tissue maintaining, as well as of its repair and regeneration. There are generally
quiescent ce progenitors, but, alter activation, they generate a population of myoblasts which proliferate and
differentiate, to form plurinucleate myotubes. Satellite cells are different, if compared to myoblasts, for
biochemical and biological characteristics. Skeletal muscle satellite cells have been also reported to have a
certain plasticity and consequent differentiation capabilites directed to different cell lineages, including
myocytes. These observations, together with autologous cell utilization, addressed the interest to the possible
use of differentiated satellite cells in altered cardiac tissue repair. The experiments have been carried out on
ovine cells, which, after in vitro amplification, have been inoculated in the same animal, undergoing an
experimentally induced cardiac lesion. The results demonstrated a ce scarce capability to repair the altered
tissue, probably because of an impaired integration with cardiomyocytes.
ID nr. 3538
Sassera D, Beninati T, Bandi C, Bouman EAP, Sacchi L, Fabbi° M, Lo N
Candidatus Midichloria mitochondrii', an endosymbiont of the tick Ixodes ricinus with a unique
intramitochondriai lifestyle
Int J Syst Evol Microbiol. - Vol. 56 ( 2006). - p 2535-2540. - 24 bib ref
Impact factor: 2,744
Project PRF2004301 - Diagnosis of avian influenza and investigation into gene exchange between animal
and human influenza virus strains. - Capua Dr.ssa Ilaria - UO - MINSAN - 2004 - 239200,00 EUR
- 02/05/2005 0.00.00 - 01/09/2007 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia
Pag. 12 di 300 31/08/2011 Specializzata - Cordioli Dr. Paolo - I
An intracellular bacterium with the unique ability to enter mitochondria exists in the European vector of Lyme
disease, the hard tick Ixodes ricinus. Previous phylogenetic analyses based on 16S rRNA gene sequences
suggested that the bacterium formed a divergent lineage within the Rickettsiales (Alphaproteobacteria). Here,
we present additional phylogenetic evidence, based on the gyrB gene sequence, that confirms the
phylogenetic position of the bacterium. Based on these data, as well as electron microscopy (EM), in situ
hybridization and other observations, we propose the name Candidatus Midichloria mitochondrii' for this
bacterium. The symbiont appears to be ubiquitous in females of 1. ricinus across the tick's distribution, while
lower prevalence is observed in males (44%). Based on EM and in situ hybridization studies, the presence of
'Candidatus M. mitochondrii' in females appears to be restricted to ovarian cells. The bacterium was found to
be localized both in the cytoplasm and in the intermembrane space of the mitochondria of ovarian cells.
'Candidatus M. mitochondrii' is the first bacterium to be identified that resides within animal mitochondria.
ID nr. 3557
Bochkov YA, Tosi° P, Massi° P, Drygin VV
Phylogenetic analysis of partial S1 and N gene sequences of infectious bronchitis virus isolates from
Italy revealed genetic diversity and recombination
Virus Gen. - Vol. 35 ( 2007). - p 65-71. - 35 bib ref
Impact factor: 1,102
Project PRC2004010 - Molecular characterization of viral variants responsible for diffusive infections of
domestic animals - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2004 - 175000,00 EUR 01/07/2005 0.00.00 - 31/12/2007 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Fallacara
Dr.ssa Francesca - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
3 - N8 - Reggio Emilia - Dottori Dr. Michele - I
4 - N3 - Forlì - Massi Dr.ssa Paola - I
A total of ten infectious bronchitis virus (IBV) isolates collected from commercial chickens in Italy in 1999 were
characterized by RT-PCR and sequencing of the S1 and N genes. Phylogenetic analysis based on partial S1
gene sequences showed that five field viruses clustered together with 793/B-type strains, having 91.3—98.5%
nucleotide identity within the group, and one isolate had very close sequence relationship (94.6% identity) with
624/I strain. These two IBV types have been identified in Italy previously. The other three variant isolates
formed novel genotype detected recently in many countries of Western Europe. For one of these variant
viruses, Italy-02, which afterwards became the prototype strain, the entire S1 gene was sequenced to confirm
its originality. In contrast, phylogenetic analysis of more conserved partial N gene sequences, comprising 1—
300 nucleotides, revealed different clustering. Thus, three variant IBVs of novel Italy-02 genotype, which had
96.7—99.2% S1 gene nucleotide identity with each other, belonged to three separate subgroups based on N
gene sequences. 624/I-type isolate Italy-06 together with Italy-03, which was undetectable using S1 gene
primers, shared 97.7% and 99.3% identity, respectively, in N gene region with vaccine strain H120. Only one
of the 793/B-type isolates, Italy-10, clustered with the 793/B strain sharing 99.3% partial N gene identity,
whereas the other four isolates were genetically distant from them (only 87.7—89.7% identity) and formed
separate homogenous subgroup. The results demonstrated that both mutations and recombination events
could contribute to the genetic diversity of the Italian isolates.
ID nr. 3561
Cattoli G, De_Battisti C, Toffan A, Salviato A, Lavazza° A, Cerioli° M, Capua I
Co-circulation of distinct genetic lineages of astroviruses in turkeys and guinea fowl
Arch Virol. - Vol. 152 ( 2007). - p 595-602. - 24 bib ref
Impact factor: 1,85
Pag. 13 di 300 31/08/2011 Viruses belonging to the genus Astrovirus have been increasingly associated with enteritis in mammalian and
avian species, including turkeys. More recently, astroviruses have also been detected in diseased guinea
fowl. In turkeys, two genetically distinct types of astrovirus have been reported, namely turkey astrovirus 1 and
2 (TAstV1 and TAstV2). The prevalence and the pathogenesis of astrovirus infections in this species is
currently unknown, with the exception of data generated in the USA. In the present report, we have
demonstrated the co-circulation of distinct genetic lineages of astroviruses infecting turkeys and guinea fowl.
The predominant lineage infecting turkeys is genetically related to the American TAstV2, but genetic variability
within this lineage was demonstrated. Other isolates appeared to be either TastV1-related or unrelated to any
other known isolate. Astroviruses infecting guinea fowl were more closely related to TAstV2, and interspecies
transmissions between turkeys and guinea fowl is suggested as a possible mechanism by which these viruses
may have evolved, based on the genetic data available. This investigation provides genetic and
epidemiological information contributing to a better understanding of enteric viral infections in turkeys and
guinea fowl.
ID nr. 3567
Paltrinieri S, Spagnolo V, Giordano A, Moreno_Martin° A, Luppi° A
Influenza virus tye A serosurvey in cats
Emerg Inf Dis. - Vol. 13 no 4 ( 2007). - p 662-664. - 10 bib ref
Impact factor: 5,094
ID nr. 3568
Parida S, Fleming L, Gibson D, Hamblin PA, Grazioli° S, Brocchi° E, Paton DJ
Bovine serum panel for evaluating foot-and-mouth disease virus nonstructural protein antibody tests
J Vet Diagn Investig. - Vol. 19 no 5 ( 2007). - p 539-544. - 18 bib ref
Impact factor: 1,145
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or
contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination.
Virus replication and persistente had been characterized in all of the animals. The proportion of the sera
scored positive by 5 tests for ant"bodies to the nonstructural proteins of FMDV varied, suggesting that the
panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of
this panel will help in assessment of new tests and quality control of existing methods.
ID nr. 3579
Bonilauri° P, Merialdi° G, Dottori° M, Barbieri° I
Presence of PRRSV in wild boar in Italy
Vet Rec. - Vol. 158 no 3 ( 2006). - p 107-108. - 1 bib ref
Impact factor: 1,017
ID nr. 3580
Circella E, Camarda A, Martella V, Bruni G, Lavazza° A, Buonavagoglia G
Coronavirus associated with an enteric syndrome on a quail farm
Avian Pathol. - Vol. 36 n 3 ( 2007). - p 251-258. - 36 bib ref
Pag. 14 di 300 31/08/2011 Impact factor: 1,809
An enteric syndrome was observed in quail (Coturnix coturnix) semi-intensively reared for restocking in Apulia
southern Italy ). The birds showed depression, severe diarrhoea, dehydration and reduced growth. mortality
occurred particularly in young birds. At necropsy the prominent lesion was enteritis. A coronavirus was
detected by electron microscopy and reverse transcriptase-polymerase chain reaction in the faeces and in the
intestinal content of the dead quails. The virus could not be cultivated in chicken embryos. By sequence
analyses of a fragment (409 nucleotides) of region lb of the polymerase gene, the quail coronavirus displayed
=93% nucleotide identity to avian coronaviruses (group 3 coronaviruses)—whereas by analysis of the Sl
portion of the spike protein-encoding gene, the quail coronavirus displayed 16°% to 18% amino acid identity
with infectious bronchitis virus. and 79% to 81% identity with turkey coronavirus. Altogether, the findings
suggest the existence of a novel coronavirus genetically related to turkey coronavirus.
ID nr. 3583
Terregino C, De_Nardi R, Guberti V, Scremin M, Raffini° E, Moreno_Martin° A, Cattoli G,
Bonfanti l, Capua I
Active surveillance for avian influenza viruses in wild birds and backyard flocks in Northern Italy
during 2004 to 2006
Avian Pathol. - Vol. 36 n 4 ( 2007). - p 337-344. - 28 bib ref
Impact factor: 1,809
Following the avian influenza epidemics that occurred in Italy between 1997 and 2003, the Italian Ministry of
Health in collaboration with veterinary authorities promoted, funded and implemented a national surveillance
programme. The main objectives of the surveillance effort were to identify avian influenza viruses circulating in
wild birds and to investigate the role of backyard poultry flocks in the dynamics of infection in a densely
populated poultry area. Over 2 years (2004 to 2006), 164 backyard flocks and 4083 wild birds (mainly
migratory Anseriformes and Charadriiformes) were sampled in three regions in the North of Italy. Samples
collected were screened by means of real-time reverse transcriptase-polymerase chain reaction and the
positive samples were processed for attempted virus isolation in embryonated fowl’s specific pathogen free
eggs. At the end of the study period, 27 low-pathogenic avian influenza viruses had been isolated from
backyard flocks and 49 strains obtained from wild birds. Of these, 26 belonged to the H5 or H7 subtype and
were closely related to contemporary low-pathogenic strains of Eurasian lineage. The findings confirm that
backyard free-range farming is at high risk for avian influenza virus introduction, and confirm the role of wild
waterfowl in the introduction and perpetuation of low-pathogenic avian influenza viruses during the winter
season in Southern Europe.
ID nr. 3587
Recordati C, Gualdi° V, Tosi S, Vailati_Facchini R, Pengo G, Luini° M, Simpson KW,
Scanziani E
Detection of Helicobacter spp. DNA in the oral cavity of dogs
Vet Microbiol. - Vol. 119 ( 2007). - p 346-351. - 27 bib ref
Impact factor: 2,073
The mode of acquisition of gastric Helicobacter spp. infection in dogs has not been determined. It is
suspected that oral–oral and faecal–oral transmission may be involved. The present study sought to
determine if Helicobacter spp. DNA is present in the oral cavity of healthy and vomiting dogs. Thirty-eight pet
dogs (27 vomiting and 11 clinically healthy) were studied. The presente of Helicobacter spp. was determined
by single and nested PCR evaluation of DNA extracted from saliva, dental plaque and gastric biopsy samples.
Helicobacter spp. DNA was detected by nested PCR in 36-(94.7%) gastric biopsies, 17 (44.7%) dental plaque
and 19 (50%) saliva samples out of the 38 dogs examined. Overall 27 (71.1 %) dogs screened by nested
PCR were found to harbour Helicobacter spp. DNA in the oral cavity (dental plaque and/or saliva). There was
Pag. 15 di 300 31/08/2011 no significant differente in the prevalence of Helicobacter spp. DNA in the oral cavity of vomiting and healthy
dogs, and the time from vomiting to oral sampling did not have significant impact. This study confirms the high
prevalence of gastric Helicobacter spp. infection in dogs, and reveals that Helicobacter spp. DNA is detectable
in the oral cavity of over 70% of dogs. These findings support the possibility of oral–oral transmission between
dogs and that the canine oral cavity may act as source of non-pylori Helicobacter spp. infection for humans.
ID nr. 3596
Moroni P, Pisoni G, Varisco° G, Boettcher P
Effect of intramammary infection in Bergamasca meat sheep on milk parameters and lamb growth
J Dairy Res. - Vol. 74 no 3 ( 2007). - p 340-344. - 18 bib ref
Impact factor: 1,407
Pooled milk samples from 115 Bergamasca meat sheep were collected aseptically five times from lambing Io
weaning Io determine the prevalence of intramammary infection, somatic cell counts and milk quality
parameters (protein, fat and lactose), and effects of infection on lamb weight gain. The global prevalence of
subclinical intramammary infection was 51.2%. The Staphylococcus genus was responsible (or the greatest
prevalence (53-3% among infected udders). Staphylococcus aureus was isolated in 8.4% of infected milk
samples. Infection status had significant effects on fat and protein percentage and on somatic cell count.
Lamb growth was greatest for lambs of ewes with no infection and decreased as the number of infected
samples increased. No significant differences were detected in the growth of lambs with dams infected by
different bacterial species.
ID nr. 3597
Cattaneo D, Dell'Orto V, Varisco° G, Agazzi A, Savoini G
Enrichment in n — 3 fatty acids of goat's colostrum and milk by maternal fish oil supplementation
Small Rumin Res. - Vol. 64 ( 2006). - p 22-29. - 41 bib ref
Impact factor: 0,777
Long-chain n — 3 polyunsaturated fatty acids (PUFAs), particularly docosahexaenoie acid (DHA), have
been shown to be essential during perinatal growth and development of mammals. The study was conducted
to determine whether the inclusion of fish oil in dairy goats diet during late gestation and early lactation would
increase the proportion of n — 3 PUFAs in colostral and mature mille From 3 weeks before kidding throughout
42 days of lactation, 14 dairy goats were fed either a control diet or a diet containing 1.1 % fish oil (FO).
Dietary treatment did not affect main components of colostrum, but lowered percentages of fat and lactose in
mature mille. In colostrum, FO supplementation lowered the proportions of C18:0, C18:2, C18:3 and elevated
those of C16:1 and very long-chain n — 3 PUFAs, mainly EPA (eicosapentaenoic acid) and DHA. In mature
milk, FO supplementation lowered the proportion of C 18:O and raised C 16:1 and n — 3 PUFAs (C18:3,EPA
and DHA). Estimated transfer efficiencies for EPA and DHA into mature milk were 14 and 7%, respectively.
Results suggest that goat's colostrum and milk can be enriched in n-3 PUFAs by maternal dietary fish oil
supplementation during late gestation and early lactation.
ID nr. 3614
Paci G, Lavazza° A, Ferretti M, Bagliacca M
Relationship between habitat, densities and metabolic profile in brow hares (Lepus europaeus
Pallas)
Ital J Anim Sci. - Vol. 6 no 3 ( 2007). - p 241 -255. - 25 bib ref
Impact factor: 0,172
Pag. 16 di 300 31/08/2011 È stato effettuato uno studio per conoscere le relazioni tra alcune caratteristiche ambientali, la densità di
popolazione ed i parametri ematici nella lepre. A tale scopo, 33 aree protette (Zone di Ripopolamento e
Cattura) della provincia di Firenze sono state sottoposte a monitoraggio ambientale e censimento delle lepri.
Per ogni area sono stati effettuati i seguenti rilievi ambientali: altitudine; rapporto tra aree aperte e area totale;
esposizione prevalente; natura del suolo; dotazioni idriche naturali; acclività; antropizzazione; presenza
predatori; adiacenze boschive perimetrali; presenza di alberi e cespugli; controllo caccia di frodo; controllo
predatori; coltivazioni; colture a perdere. Dopo il censimento tutte le lepri catturate sono state traslocate in
zone libere di caccia. Un totale di 172 soggetti è stato sottoposto ai seguenti rilievi: prelievo ematico dalla
vena auricolare per le analisi del glucosio, colesterolo, urea, AST, ALT, Ca, P, Mg, Na, K e Cl, determinazione
dell'età, del sesso e del peso vivo. Le relazioni fra densità di popolazione e caratteristiche ambientali sono
state analizzate singolarmente, quindi tutte le caratteristiche ambientali sono state sottoposte al processo di
selezione "stepwise" in funzione del peso corporeo rilevato nelle lepri. 1 dati ematici sono stati analizzati
mediante il metodo dei minimi quadrati considerando le caratteristiche ambientali, la densità censita, l'età ed il
sesso degli animali come fonti di variazione categoriche, l'interazione sesso * età e lo stato fisiologico delle
femmine "nested' nel sesso femminile. I risultati hanno evidenziato la presenza di una più alta densità di
popolazione e le migliori condizioni corporee nelle zone collinari caratterizzate da aree aperte con scarsi
boschetti e confini boscosi, con suolo di medio impasto, scarsa presenza di predatori, limitata antropizzazione
e abbondante disponibilità idrica naturale e zone ricche di cespugli adatti al rifugio. Lo studio dei valori
assoluti del profilo metabolico, indicatori dello stato fisiologico e nutritivo degli animali allevati, non ha
evidenziato alcuna carenza nutrizionale negli animali selvatici nel periodo invernale considerato.
Presumibilmente, come i dati dei censi-menti, devono essere ripetuti per più anni dal momento che è la
variazione ad essere utile come indicatore di stato e non il valore assoluto.
ID nr. 3616
Knowles NJ, Wilsden G, Reid SM, Ferris NP, King DP, Paton DJ, Fevereiro M, Brocchi° E
Reappearance of swine vesicular disease virus in Portogal
Vet Rec. - Vol. 161 no 2 ( 2007). - p 71
Impact factor: 1,168
Project PRC2002007 - Improvement of SVD diagnosis: identification and neutralization of swine
enteroviruses affecting SVD virus isolation in tissue cultures. - Brocchi Dr.ssa Emiliana - Capofila MINSAN - 2002 - 208749,00 EUR - 15/04/2004 0.00.00 - 31/12/2006 0.00.00 - 1 - H1 - Reparto
di Biotecnologie - Brocchi Dr.ssa Emiliana - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
3 - IZSVE - Nardelli Dr. Stefano - E
4 - ISS - Tollis Dr.ssa Maria - E
ID nr. 3621
Ferrari° M, Corradi A, Lazzaretti M, De'Cillà° M, Losi° CG, Villa° R, Lanfranchi° A
Adult stem cells : perspectives for therapeutic applications
Vet Res Commun. - Vol. 31 suppl 1 ( 2007). - p 1-8. - 17 bib ref
Impact factor: 0,377
The use of adult stem cells in tissue regeneration appears to be a powerful research tool, due to the intrinsic
characteristics of these cells, i.e., self-renewal and unlimited capacity for proliferation. In particular,
mesenchymal stem cells (MSCs) obtained from bone marrow or peripheral blood can be easily isolated,
cultivated, propagated and can be differentiated into several specialized celi types thanks to their plasticity.
Among these cells, MSCs can evolve into cardiac cell lineages. Since heart damage leads to the irreversible
loss of cardiac function, cell transplantation could be a potential therapy for heart injury. Our laboratory has
focused on the purification and expansion of rat and sheep MSCs, their differentiation into cardiomyocytes
and their characterisation. Numerous results indicate that MSCs could be promising for therapy, however we
need to better understand the biology of stem cells to improve methods for delivery and/or pharmacological
activation. These techniques can indeed track engrafted cells and systems to guarantee their safe use.
Pag. 17 di 300 31/08/2011 ID nr. 3622
Goris N, Praet N, Sammin D, Yadin H, Paton D, Brocchi° E, Berkvens D, De_Clercq K
Foot-and-mouth disease non-structural protein serology in cattle: use of a Bayesian framework to
estimate diagnostic sensitivity and specificity of six ELISA tests and true prevalence in the field
Vaccine. - Vol. 25 no 41 ( 2007). - p 7177-7196. - 29 bib ref
Impact factor: 3,159
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
The diagnostic performance of six foot-and-mouth disease (FMD) assays for detection of antibodies to the
non-structural proteins (NSP) of the FMD virus (FMDV) was estimated using a Bayesian analysis on field sera
from cattle of unknown infection status originating from post-FMDV outbreak situations in Israel and
Zimbabwe. Estimations of the disease prevalence in both populations were also obtained. The diagnostic
sensitivity estimates did not differ between both field studies, although overall Bayesian estimates were
markedly higher than those previously reported based on sera from comparable experimentally infected
(vaccinated) cattle populations. All NSP-based assays demonstrated a lower diagnostic specificity when
applied to the Zimbabwean sera compared to both published specificities and similar Bayesian specificity
estimates derived for the Israeli dataset. In Israel, the disease prevalence was estimated at 23.9% (95%
credibility interval: 19.5-28.8%), whereas 65.4% (59.0-72.5%) was found in Zimbabwe. The need for reliable
diagnostic test performance estimates and the benefits of Bayesian analysis in obtaining them are also
addressed.
ID nr. 3623
Roncada P, Begni° B, Amadori° M, Cristoni S, Archetti° IL, Boldetti° C, Fortin R, Deriu F,
Greppi GF
Blood serum proteome for welfare evaluation in pigs
Vet Res Commun. - Vol. 31 suppl 1 ( 2007). - p 321-325. - 4 bib ref
Impact factor: 0,377
ID nr. 3624
Bonardi S, Paris A, Bacci C, D'Incau° M, Ferroni L, Brindani F
Detection and characterization of Yersinia enterocolitica from pigs and cattle
Vet Res Commun. - Vol. 31 suppl 1 ( 2007). - p 347-350 - 9 bib ref
Impact factor: 0,377
ID nr. 3625
Ghidini S, Zanardi E, Conter M, Varisco° G, Bolzoni° G, Ianieri A
Residues of aminoglycosides in milk : confirmatory analysis
Vet Res Commun. - Vol. 31 suppl 1 ( 2007). - p 365-367 - 2 bib ref
Impact factor: 0,377
Project PRC2004001 - Design, development and production of reference samples with known content for
the inhibiting-substances assay in milk and validation of a test procedure for cheese - Bolzoni Dr.
Giuseppe - Solo - MINSAN - 2004 - 45000,00 EUR - 01/07/2005 0.00.00 - 31/12/2006 0.00.00 1 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti - Varisco
Dr. Giorgio - I
Pag. 18 di 300 31/08/2011 2 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti Bertocchi Dr. Luigi - I
3 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti - Bolzoni
Dr. Giuseppe - I
ID nr. 3628
Bonardi S, Foni° E, Chiapponi° C, Salsi A, Brindani F
Detection of verocytotoxin-producing Escherichia coli serogroups O157 and O26 in the cecal content
and lymphatic tissue of cattle at slaughter in Italy
J Food Prot. - Vol. 70 no 6 ( 2007). - p 1493-1497. - 30 bib ref
Impact factor: 1,921
Verocytotoxin-producing Escherichia coli (VTEC) has emerged as a foodborne pathogen that can cause
severe and potentially fatal illnesses, such as hemorrhagic colitis or the hemolvtie uremie syndrome. In this
study, 182 cattle at slaughter (119 dairy cows and 63 feedlot cattle) were randomly selected and tested for the
presence of VTEC serogroups 026, 0103, 0111, 0145, and 0157 in their cecal content and lymphatic tissue
(tonsils or mesenteric lymph nodes). A total of 364 samples were evaluated with an immunomagnetic
separation technique followed by slide agglutination. Presumptive VTEC 026. 0103, 0111, 0145, and 0157
isolates were tested by Vero cell assay for verocvtotoxin production and by multiplex PCR assay for the
detection of vtx1, vtx2 eae, and E-hlyA genes. VTEC 0157 was detected in 6 (3.3%o) of 182 animals, and
VTEC 026 was detected in 1 (0.5%) of 182 animals. No VTEC 0103. VTEC 0111, or VTEC 0145 isolates were
found in cattle feces, but one VTEC 091:H- vtx2(+in apice) eae(- in apice), E-hlyA(-in apice) strain
nonspecifically cross-reacted with the VTEC 0103 type. The prevalence of VTEC 0157 in the lymphatic tissue
of cattle was 1.107° in both tonsils (1 of 93 samples) and mesenteric lymph nodes (1 of 89 samples).
Lymphatic tissue contamination was observed only in VTEC 0157 intestinal carriers: two (33.3%) of six fecal
carriers were simultaneously VTEC 0157 lymphatic carriers. This finding suggests that VTEC 0157
contamination of meat does not necessarily come from feces or the environment. No other VTEC serogroups
were detected in the lymphatic tissue of slaughtered cattle.
ID nr. 3634
Campitelli L, Di_Martino A, Spagnolo D, Gavin JDS, Di_Trani L, Facchini M, De_Marco
MA, Foni° E, Chiapponi° C, Moreno_Martin° A, Chen H, Guan Y, Delogu M, Donatelli I
Molecular analysis of avian H7 influenza viruses circulating in Eurasia in 1999-2005: detection of
multiple reassortant virus genotypes
J Gen Virol. - Vol. 89 no 1 ( 2008). - p 48-59. - 60 bib ref
Impact factor: 3,120
Project CE ISS FLUPAN - Preparing for an influenza Pandemic (Flupan) - Donatelli Dr.ssa Isabella - UO CE - 2001 - 99999999,00 EUR - 23/04/2002 0.00.00 - 30/06/2004 0.00.00 - 2 - N5 - Parma Barigazzi Dr. Giuseppe - I
3 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
PRC99007 - Epidemiological surveillance of influenza virus re-circulation in domestic and wild
animals: an investigation into antigenic characterization of circulating strains in susceptible domestic
animal species (pig, horse, avian species), intensification of surveillance in wild avian species and
from rural flocks, new immunizing strategies for swine - Barigazzi Dr. Giuseppe - Capofila MINSAN - 1999 - 01/03/2001 0.00.00 - 28/08/2003 0.00.00 - 347643000,00 LIT 1 - N5 - Parma Foni Dr.ssa Emanuela - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
3 - N3 - Forlì - Massi Dr.ssa Paola - I
4 - ISS - Donatelli Dr.ssa Isabella - E
5 - INFS - De Marco Dr.ssa Maria Alessandra - E
6 - ISS 2 - Castrucci Dr.ssa Maria Rita - E
Avian influenza infections by high and low pathogenicity H7 influenza viruses have caused several outbreaks
Pag. 19 di 300 31/08/2011 in European poultry in recent years, also resulting in human infections. Although in some cases the source of
H7 strains from domestic poultry was shown to be the viruses circulating in the wild bird reservoir, a thorough
characterization of the entire genome of H7 viruses from both wild and domestic Eurasian birds, and their
evolutionary relationships, has not been conducted. In our study, we have analysed low pathogenicity H7
influenza strains isolated from wild and domestic ducks in Italy and southern China and compared them with
those from reared terrestrial poultry such as chicken and turkey. Phylogenetic analysis demonstrated that the
H7 haemaggiutinin genes were all closely related to each other, whereas the remaining genes couid be
divided into two or more phylogenetic groups. Almost each year different H7 reassortant viruses were
identified and in at least two different years more than one H7 genotype co-circulated. A recent precursor in
wild waterfowl was identified for most of the gene segments of terrestrial poultry viruses. Our data suggest
that reassortment allows avian influenza viruses, in their natural reservoir, to increase their genetic diversity.
In turn this might help avian influenza viruses colonize a wider range of hosts, including domestic poultry.
ID nr. 3638
Decaro N, Mari M, Desario C, Campolo M, Elia G, Martella V, Greco G, Cirone F,
Colaianni ML, Cordioli° P, Buonavoglia C
Severe outbreak of bovine coronavirus infection in dairy cattle during the warmer season
Vet Microbiol. - Vol. 126 ( 2008). - p 30-39. - 31 bib ref
Impact factor: 2,010
Project PRF2007202 - Mammal coronaviruses: molecular epidemiology, vaccine development And
implications for animal and human health - Cordioli Dr. Paolo - Capofila - MINSAN - 2007 300000,00 EUR - 01/09/2009 0.00.00 - 31/08/2011 0.00.00 - 1 - U1 - REPARTO VIROLOGIA Cordioli Dr. Paolo - I
2 - Università di Bari - Dipartimento di Sanità Pubblica e Zootecnica - Decaro Prof. Nicola - E
3 - IstitutoZooprofilattico della Sardegna - Oggiano Dr.ssa Annalisa - E
4 - Istituto Zooprofilattico della Sicilia - Guercio Dr.ssa Annalisa - E
5 - Istituo Zooprofilattico delle Venezie - Nardelli Dr. Stefano - E
A severe outbreak of enteric and respiratory disease associated with bovine coronavirus (BCoV) infection is
described. The outbreak occurred in a dairy herd of southern Italy in the first decade of September 2006,
when summer temperatures were still recorded, affecting calves, heifers and adult cows, with a marked
decrease in milk production. By virus isolation and RT-PCR targeting the S gene, BCoV was identified as the
etiological agent of the outbreak, whereas bacteriological, parasitological and toxicological investigations
failed to detect other causes of disease. BCoV strains with 99-100% nucleotide identity in the S gene were
isolated from nasal, ocular and rectal swabs, thus proving the absence of separate clusters of virus on the
basis of tissue tropism. Sequence analysis of the haemagglutination-esterase and spike proteins of the strain
detected in one rectal sample (3391 06) showed a high genetic relatedness with recent BCoV isolates (9899% amino acid identity), with several unique amino acid substitutions in the S protein. The BCoV outbreak
described in this paper presents interesting aspects: (i) the occurrence of a severe form of disease in the
warmer season; (ii) the simultaneous presente of respiratory and enteric disease; (iii) the involvement of
young as well as adult cattle.
ID nr. 3639
Bellini° S, Santucci U, Zanardi° G, Brocchi° E, Marabelli R
Swine vesicular disease surveillance and eradication activities in Italy
Rev Sci Tech OIE. - Vol. 26 no 3 ( 2007). - p 585-593. - 20 bib ref
Impact factor: 0,744
Project PRC2002007 - Improvement of SVD diagnosis: identification and neutralization of swine
enteroviruses affecting SVD virus isolation in tissue cultures. - Brocchi Dr.ssa Emiliana - Capofila MINSAN - 2002 - 208749,00 EUR - 15/04/2004 0.00.00 - 31/12/2006 0.00.00 - 1 - H1 - Reparto
di Biotecnologie - Brocchi Dr.ssa Emiliana - I
Pag. 20 di 300 31/08/2011 2 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
3 - IZSVE - Nardelli Dr. Stefano - E
4 - ISS - Tollis Dr.ssa Maria - E
Swine vesicular disease (SVD) was first observed in Italy in 1966, and was initially diagnosed as foot and
mouth disease (FMD). The causative agent of SVD was classified as an Enterovirus within the family
Picornaviridae. It was included in the list of diseases notifiable to the World Organisation for Animail Health
(OIE) because of the similarity of its lesions to those produced by FMD; however SVD is often mild in nature
and may infect pigs subclinically.
During the fast decade SVD has been persistently reported in Italy, and surveillance and eradication activities
are in place. The central and northern parts of Italy have been designated SVD free since 1997, while the
southern regions have not achieved disease-free status. However, occasional outbreaks of SVD have
occurred in central and northern Italy and have been eradicated using rigorous control measures. Most recent
SVD outbreaks in Italy have been subclinical; SVD can rarely be diagnosed now on the basis of clinical signs
and it is necessary to use laboratory diagnosis. This paper examines the epidemiology of SVD in Italy, and
considers the measures adopted in Europe for SVD control on the basis of current knowledge of the disease.
ID nr. 3641
Zavanella M, Muliari R, Mioni R, D'Incau° M
Microbes and foods
Microbi e alimenti / Fondazione Iniziative Zooprofilattiche e Zootecniche Brescia. - Brescia : Fondazione
Iniziative Zooprofilattiche e Zootecniche, 2008. - (Atti delle Giornate di Studio Fondazione Iniziative
Zooprofilattiche e Zootecniche ; 70) p 1-283
The book describes the principles of food microbiology and the main laboratory methods. It also contains an
overview of the main rules concerning food hygiene and control..
ID nr. 3642
Nordengrahn A, Gustafsdottir SM, Ebert K, Reid SM, King DP, Ferris NP, Brocchi° E,
Grazioli° S, Landegren U, Merza M
Evaluation of a novel proximity ligation assay for the sensitive and rapid detection of foot-and-mouth
disease virus
Vet Microbiol. - Vol. 127 ( 2008). - p 227-236. - 14 bib ref
Impact factor: 2,010
Project 2003-SSP-3-513645 - New and emerging technologies: improved laboratory and on-site detection of
oie list a viruses in animals and animal products (LAB-ON.SITE) - Belak Dr. Sandor - UO - CE 2004 - 1500000,00 EUR - 01/11/2004 0.00.00 - 31/07/2008 0.00.00 - 7 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
A novel proximity ligation assay (PLA) using a pan-serotype reactive monoclonal antibody was developed and
evaluated for the detection of foot-and-mouth disease virus (FMDV) in clinical samples collected from field
cases of disease. The FMDV-specific PLA was found to be 100 times more sensitive for virus detection than
the commonly used antigen capture-ELISA (AgELISA). As few as five TCID50 were detected in individual
assays, which was comparable with the analytical sensitivity of real-time RT-PCR. Although this assay was
capable of detecting diverse isolates from all seven FMDV serotypes, the diagnostic sensitivity of the PLA
assay was lower than real-time RT-PCR mainly due to a failure to detect some SAT 1, SAT 2 and SAT 3
FMDV strains. In conclusion, this new PLA format has high analytical sensitivity for the detection of FMDV in
clinical samples and may prove valuable as a rapid and simple tool for use in FMD diagnosis.
Pag. 21 di 300 31/08/2011 ID nr. 3643
Stacchiotti A, Lavazza° A, Ferroni M, Sberveglieri G, Bianchi R, Rezzani R, Rodella LF
Effects of aluminium sulphate in the mouse liver : smilarities to the aging process
Exp Gerontol. - Vol. 43 ( 2008). - p 330-338. - 36 bib ref
Impact factor: 2,879
Aluminium (Al) is a ubiquitous metal that is potentially toxic to the brain. Its effects on other fundamental
organs are not completely understood. This morphological in vivo study sought to compare sublethal
hepatotoxic changes and Al deposition in adult mice that orally ingested Al sulphate daily for 10 months, in
age matched control mice that drank tap water and in senescent mice (24 months old). Livers were examined
for collagen deposition using Sirius red and Masson, for iron accumulation using Perls' stain. Light, electron
microscopy and morphometry were used to assess fibrosis and vascular changes. Scanning transmission
electron microscopy and EDX microanalysis were used to detect in situ elemental Al. Iron deposition,
transferrin receptor expression were significantly altered following Al exposure and in the aged liver but were
unaffected in age matched control mice. In Al treated mice as in senescent mice, endothelial thickness was
increased and porosity was decreased like perisinusoidal actin. Furthermore, Al stimulated the deposition of
collagen and laminin, mainly in acinar zones 1 and 3. Pseudocapillarization and periportal laminin in
senescent mice were similar to Al treated adult liver. In conclusion, prolonged Al sulphate intake accelerates
features of senescence in the adult mice liver.
ID nr. 3645
Decaro N, Desario C, Elia G, Martella V, Mari V, Lavazza° A, Nardi M, Buonavoglia C
Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c
New microbiol. - Vol. 31 ( 2008). - p 125-130. - 38 bib ref
Impact factor: 0,956
An outbreak of canine panvovirus type 2c (CPV-2c) infection in vaccinated adult dogs is reported. The
disease occurred in a breeding kennel in Italy and affected 11 dogs aged between 6 months and 2.5 years,
that had been repeatedly administered vaccines containing a type 2 (old type) CPV strain. CPV infection was
demonstrated in all diseased dogs by an immunochromatographic test. A CPV strain was isolated from the
intestinal content of a 20-monthold pregnant Bernese mountain bitch that underwent a fatal outcome. The
strain was characterised as CPV-2c by means of real-time PCR assays using minor groove binder probes.
The present report provides further concerns about the real efficacy of type 2-based vaccines against the
antigenic variants of CPV and stresses the need for developing new vaccines prepared with the variants
currently circulating in the dog population.
ID nr. 3646
Moreno_Martin° A, Fallacara° F, Barbieri° I, Tosi° G, Rivallan G, Eterradossi N, Ceruti R,
Cordioli° P
Genetic and antigenic characterization of infectious bursal disease viruses isolated in Italy during
the period 2002-2005
Avian Dis. - Vol. 51 n. 4 ( 2007). - p. 863-872
Impact factor: 1,369
Project PRC2004010 - Molecular characterization of viral variants responsible for diffusive infections of
domestic animals - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2004 - 175000,00 EUR 01/07/2005 0.00.00 - 31/12/2007 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Fallacara
Dr.ssa Francesca - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
3 - N8 - Reggio Emilia - Dottori Dr. Michele - I
4 - N3 - Forlì - Massi Dr.ssa Paola - I
Pag. 22 di 300 31/08/2011 During the period 2002–2005, 109 infectious bursal disease virus (IBDV) field strains were isolated from bird
flocks located in various parts of Italy. Out of these strains, 91 were isolated from broilers, 12 from pullets, and
six from backyard flocks. Forty-two IBDV strains were further investigated and characterized of the basis of
the geographical origin, source, and clinical signs. Antigenic and genetic characterizations were carried out
using a monoclonal antibody (MAb)–based antigen-capture (AC) enzyme-linked immunosorbent assay
(ELISA) or a virus neutralization assay and. a reverse transcription, amplification, and direct sequencing of a
genome fragment encoding the VP2 variable domain. The viruses were compared with reference IBDV
strains, F52/70 (classical, 1970), 89163 (typical very virulent [vv]IBDV, 1989), 91168 (antigenically modified
vvIBDV, 1991) and 94432 (antigenically modified vvIBDV, 1994) among others. Alt 42 strains were genetically
characterized, and the comparison of their nucleotide sequences revealed the presence of six clusters having
100% identity, named group 1, 2, 3, 4, 5, and 6. Twelve strains, representative of each molecular group
and/or with interesting amino acid sequence, were also antigenically characterized. The antigenic
characterization showed six strains-151573, 157185 (group 1), 192294 (group 2), 77882 (group 3), 217 (group
4), and 192304—with the profile rypical of vvIBDV (lack of binding of MAbs 3 and 4). Two strains, 77165 and
204875 (group 6), were also related to vvIBDV but did not react with MAb 5. Three isolates exhibited a profile
of cell culture–adapted viruses and classical strains but with some differences: strain 157776 reacted with alt
MAbs; strain 168026 with alt MAbs except MAb 4, which weakly neutralized it; and strain 72293 with alt MAbs
except MAb 9, which is rather unusual. The last strain, 213622, showed a very uncommon antigenic profile
with missing or reduced binding of MAbs 3, 4, 5, 6, 8, and 9. Genetic characterization revealed 37 strains
identified as vvIBDV viruses divided in 26 isolates (including groups 1, 2, 3, and 4) with the four amino acids
residues rypical of vvIBDV (222A, 256I, 294I, 2995) and 11 isolates (including groups 5, 6, and 213622) with
some other amino acid exchanges. Four isolates (72293, 168026, 196783, and 222220) presented an amino
acid sequence closely related to attenuate classical viruses whereas the Cast isolate (157776) exhibited a
rather different sequence with some mutations rypical of vvIBDV and others for cell culture–adapted viruses.
Results of the antigenic and genetic characterization revealed that the majority of viruses (n = 37) were
related to vvIBDV strains but, among these, 11 strains presented antigenically and genetically modified
characteristics and originated, in major part, from the area where viruses have been circulating for a long time.
The remaining viruses (n = 5) were related but not identical to attenuated classical viruses and came from
areas where vaccination with intermediate strains is applied.
ID nr. 3647
Cesari V, Toschi I, Ferrazzi V, Cesari N, Grilli G, Lavazza° A
Effect of weaning age and diet on growth performance, caecal characteristics and potential
pathogenetic microflora in rabbits
Ital J Anim Sci. - Vol. 6 suppl 1 ( 2007). - p 755-757. - 6 bib ref
Impact factor: 0,172
Project RELO2003596CUNIBENE - Welfare of farmed rabbits: associated sanitary and production traits Lavazza Dr. Antonio - Capofila - REGIONE - 2003 - 149325,00 EUR - 01/10/2003 0.00.00 29/01/2007 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza Dr.
Antonio - I
2 - UNIV MILANO Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione
di Anat - Grilli Dr. Guido - E
RELO2004685CUNISVEZZ - Influence of weaning age and diet on productive performance,
prevention of digestive diseases and welfare of the young rabbit - Toschi Dr. Ivan - UO - REGIONE
- 2004 - 04/10/2004 0.00.00 - 31/01/2007 0.00.00 - 92817,00 EUR 1 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
The aim of this study was to investigate the effect of age and diet at weaning on growth performances, caecal
characteristics and development of potential pathogenetic microflora. The trial was carried out on 64 litters
from 18 to 45 days of age comparing two weaning age (25 vs. 34 d) and two weaning diets (HF and LF)
characterized, especially, by different energy, fat and starch concentration (HF: DE=11.5 MJ/kg, EE=6.270,
Starch=8.770; LF: DE=10.1 MJ7kg DM, EE=3.1'1-, Starch=11.37x-). At 45 d, early weaning determined a
significant reduction of growth (1219 vs 1282; P<0.01), although the solid feed intake resulted significantly
higher, from 25 to 34 days of age, in early weaned rabbits (50.0 us 29.8 g/d; P<0.001). The age at weaning
Pag. 23 di 300 31/08/2011 did not modify caecal traits, witb the exception of pH value, that was lower in rabbits early moved to the
mothers (5.53 us 5.83; P<0,05). Independently by age of weaning, the administration of diet HF promoted
growth performance in rabbits at 45 d (1280 vs 1222; P<0.01) Diet composition did not influence mortality rate
at 34 days of age, while early weaning determined values significantly higher than those found in rabbits
weaned later. The data of microbial analysis, carried out on 10 animals per diet (at 25 and 34 days of age),
showed potential pathogenetic microflora development depended by the age of animal and was not affected
by the composition of diets.
ID nr. 3648
Circella E, Martella V, Bruni G, Lorusso E, Lavazza° A, Camarda A,
Coronavirus-associated with an enteritis in a quail farm
Ital J Anim Sci. - Vol. 6 n 3 ( 2007). - p 326-329. - 9 bib ref
Impact factor: 0,172
An enteric syndrome was observed in quail (Coturnix coturnix) semi-intensively reared for restocking in Apulia
southern Italy ). The birds showed depression, severe diarrhoea, dehydration and reduced growth. mortality
occurred particularly in young birds. At necropsy the prominent lesion was enteritis. A coronavirus was
detected by electron microscopy and reverse transcriptase-polymerase chain reaction in the faeces and in the
intestinal content of the dead quails. The virus could not be cultivated in chicken embryos. By sequence
analyses of a fragment (409 nucleotides) of region lb of the polymerase gene, the quail coronavirus displayed
=93% nucleotide identity to avian coronaviruses (group 3 coronaviruses)—whereas by analysis of the Sl
portion of the spike protein-encoding gene, the quail coronavirus displayed 16°% to 18% amino acid identity
with infectious bronchitis virus. and 79% to 81% identity with turkey coronavirus. Altogether, the findings
suggest the existence of a novel coronavirus genetically related to turkey coronavirus.
ID nr. 3649
Martella V, Bànyai K, Lorusso E, Bellacicco AL, Decaro N, Camero M, Bozzo G,
Moschidou P, Arista S, Pezzotti G, Lavazza° A, Buonavoglia C
Prevalence of group C rotaviruses in weaning and post-weaning pigs with enteritis
Vet Microbiol. - Vol. 123 ( 2007). - p 26-33. - 47 bib ref
Impact factor: 2,073
Diarrheic fecal specimens collected from porcine herds were screened for the presence of group C
rotaviruses using a reverse transcription-polymerase chain reaction (RT-PCR) assay. A total of 188 samples
were tested and 54 were positive. When compiled these data with diagnostic results on group A rotaviruses
and enteric caliciviruses we found that all but 5 group C rotavirus positive samples contained at least one
additional virus. A subset of samples were subjected to nucleotide sequencing. The selected strains showed
an unexpectedly wide range of nucleotide sequence heterogeneity (88.6-100%) to each other and to the
reference porcine group C rotavirus strain, Cowden. The nucleotide sequence identity to the genuine bovine
and human strains were, respectively, 86.8 and 87.2% or less. In conclusion, our study demonstrates that
infection with group C rotavirus is frequent in Italian piggeries. The considerable rate of multiple infections
requires further studies to investigate the pathogenic potential of group C rotaviruses in pigs, alone or in
mixed infection, and raises challenges in the laboratory diagnosis of porcine enteric infections.
ID nr. 3650
Martella V, Ciarlet M, Banyai K, Lorusso E, Arista S, Lavazza° A, Pezzotti G, Decaro N,
Cavalli A, Lucente MS, Corrente M, Elia G, Camero M, Tempesta M, Buonavoglia C
Identification of Group A Porcine Rotavirus Strains Bearing a Novel VP4 (P) Genotype in Italian
Pag. 24 di 300 31/08/2011 Swine Herds
J Clin Microbiol. - Vol. 45 no 2 ( 2007). - p 577-580. - 44 bib ref
Impact factor: 3,445
The VP4 gene of a G5 Italian porcine rotavirus strain, 344/04-1, was nontypeable by PCR genotyping. Thc
amino acid sequence of the full-length VP4 protein had low identity ((piu o meno)76.6%) with the homologous
sequences of representative strains of the remaining P genotypes, providing evidente for a novel P genotype.
ID nr. 3651
Monini M, Cappuccini F, Battista P, Falcone E, Lavazza° A, Ruggeri FM
Molecular characterization of bovine rotavirus strains circulating in northern Italy, 2003-2005
Vet Microbiol. - Vol. 129 ( 2008). - p 384-389. - 28 bib ref
Impact factor: 2,010
Project PRF2003307 - Genetic and antigenic diversity of rotaviruses – study of the evolutionary mechanisms
and implications for diagnosis and vaccination - Gentile Dr. Mattia - UO - MINSAN - 2003 256800,00 EUR - 01/03/2004 0.00.00 - 31/10/2006 0.00.00 - 7 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
A total of 232 stools collected from calves with rotavirus infection in herds located in northern Italy from 2003
to 2005 was investigated. Determination of the rotavirus G and P types was carried out using nested RT-PCR.
G6 was the most prevalent genotype, accounting for 78.5% of samples, G10 accounted for 9.9% of samples
and viruses of G8 type were found in 4.7% of samples. In 3% of samples, viruses were not classified due to
concomitant infection with more G type strains, whereas viruses in 3.9% of samples could not be
characterized with any of the G-specific primers used in this study. Most common P types were P[11] and
P[5], accounting for 65.1% and 25%, respectively. In 2.6% of cases, samples reacted with multiple P-specific
primers; no P[1] serotype was identified. The G6P[11] combination was predominant throughout the study
period, i.e. 52.5% in 2003, 50% in 2004 and 40% in 2005. The incidence of G6P[5] increased from 13.1 % in
2003 to 27% in 2004 and 25.5% in 2005. The G10P[11] combination decreased markedly from 18% in 2003
to 2.6% in 2004, rising again to 7.3% in 2005. G8P[11] viruses were similarly present in 2003 (5%) and 2004
(4.3%), declining slightly in 2005 (1.8%).
ID nr. 3652
Martella V, Bànyai K, Lorusso E, Decaro N, Bellacicco A, Desario C, Corrente M, Greco G,
Moschidou P, Tempesta M, Arista S, Ciarlet M, Lavazza° A, Buonavoglia C
Genetic heterogeneity in the VP7 of group C rotaviruses
Virology. - Vol. 367 ( 2007). - p 358-366. - 61 bib ref
Impact factor: 3,525
Evidence for a possible zoonotic role of group C rotaviruses (GCRVs) has been recently provided. To gain
information on the genetic relationships between human and animal GCRVs, we sequenced theVP7 gene of
10 porcine strains detected during a large surveillance study from different outbreaks of gastroenteritis in
piglets. Four GCRV strains were genetically related to the prototype GCRV porcine Cowden strain. A
completely new VP7 genotype included 4 strains (344,04-7-like) that shared 92.5% to 97.0% aa identity to
each other, but <83% to human GCRVs and <79% to other porcine and bovine GCRVs. A unique 4-aa
insertion (SSSV or SSTI), within a variable region at die carboxy-terminus of VP7, represented a distinctive
feature for these 4 unique strains. An additional strain, 134/04-1.8, was clearly different from all human and
animal GCRVs (<851/t aa ìdentity) and likely accounts for a distinct VP7 genotype. The VP7 of a unique
strain, 42/05-21, shared similar ranges of aa sequence identities with porcine and human strains (88.0- 90J%
to porcine GCRVs and 85.2 -88.2% to human GCRVs). Plotting the VP7 gene of strain 42/05-21 against the
VP7 of human and porcine strains revealed discontinuous evolution rates throughout the VP7 molecule,
Pag. 25 di 300 31/08/2011 suggesting different mutational pressure or a remote intragenic recombination event. These findings provide
the need for future epidemiological surveys and warrant studies to investigate the pathogenic potential of
these novel GCRVs in pigs.
ID nr. 3655
Frezza D, Giambra V, Chegdani F, Fontana C, Maccabiani° G, Losio° N, Faggionato° E,
Chiappini B, Vaccari G, Von_Holst C, Lanni L, Saccares S, Ajmone-Marsan P
Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs
Innov Food Sci Emerg Tech. - Vol. 9 ( 2008). - p 18-23. - 30 bib ref
Impact factor: 1,713
Project PRC2001002 - Study and application of molecular methods for the identification of animal tissues in
food and feed - Faggionato Dr.ssa Elena - Capofila - MINSAN - 2001 - 780279000,00 LIT 16/09/2002 0.00.00 - 15/09/2004 0.00.00 - 1 - I2 - Reparto Bromatologico - Faggionato Dr.ssa
Elena - I
2 - N1 - Bologna - Fontana Dr.ssa Maria Cristina - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica Losio Dr.ssa Marina Nadia - I
4 - UNI PC - Ajmone Marsan Dr. Paolo - E
5 - UNI TOR VERGATA - Frezza Dr. Domenico - E
In this work four species-specific primers and probes were designed and evaluated for the detection and
quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds. PCR primers were optimized
using conventional and Rea] Time PCR, to detect short species-specific sequences amplifiable from heat
treated matcrial. Both methods confirmted the high specificity of the primers designed. Real time quantitative
PCR assay allowed the detection of as fcw as 0.01 ng and 0.05 ng of ovine and bovine gcnomic DNA,
respectively. Tbc detection limit for swine and chicken genomie DNA was 0.5 ng. Sensitivity levels observed
in DNA extrated from meat samples proeessed according to E legislation were different compared to those in
gnomic DNAs previously described. They resulted in swine 5 ig of MBM DNA, in chicken 25 ng, in ovine and
bovine 50 ng. Wc confirmed the efficiency and specificity of primers in RT-PCR to detect 0.5% of bovine,
ovine, swine and chicken MBM in contaminated feedstuffs.
ID nr. 3698
Martinelli N°, Lombardi G°
Investigation on the presence of pathogens in laboratory mice and rats of breeding and testing
centers
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
33-34. - 3 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Martinelli Nicola
In this report prevalence rates of mice and rats pathogens in laboratory animal are presented. In mice and rats
the most detected infectious agent was M. pulmonis (43,5%) followed by mouse hepatitis virus (36,6%) in
mice and by Theiler’s virus in rats. Although health status is very important in laboratory animals, several
infectious agents are still circulating in mice and rats colony.
ID nr. 3704
Pag. 26 di 300 31/08/2011 Bosi P, Sarli G, Casini L, De_Filippi S, Trevisi P, Mazzoni M, Merialdi° G
The influence of fat protection of calcium formate on growth and intestinal defence in Escherichia
coli K88-challenged weanling pigs
Anim Feed Sci Tech. - Vol. 139 ( 2007). - p 170-185. - 38 bib ref
Impact factor: 1,29
An experiment was conducted to test whether free or fat-protected dietary calcium formate improves the
growth and health of weanling pigs that may or may not be susceptible to intestinal adhesion of
enterotoxigenic Escherichia coli (ETEC), when orally challenged with ETEC. Sixty pigs, weaned at 21 days of
age (day 0), were divided into three groups (20 pigs each), balanced for number, litter and live weight, and fed
the same base diet but three different 30.5 g/kg supplementations: a control (CO-with dicalcium phosphate,
calcium sulphate, lard and sepiolite); a free calcium formate (FF), with this additive, monosodium phosphate
and lard; a fat-protected calcium formate (PF), with this additive and monosodium phosphate only. The final
diets did not differ for total calcium and phosphorus content. The pigs, individually penned, were orally
challenged with 1.5 ml of a 1010 CFU E. coli K88 suspension on day 2 and slaughtered on day 7 or 8. Data
were analysed using analysis of variance with a three-factor design, including diet, block, sensitivity of
intestinal villus to ETEC adhesion, and first level interactions. The factor diet never interacted with the others.
Compared to the control diet, the formate supplementations improved growth (P<0.05), feed intake (P=0.062)
and GT (gain:feed) ratio (P=0.063). Both forms of formate addition reduced the faecal score (P=0.062), days
of darrhoea (P<0.05) and total E. coli faecal excretion (P<0.05) but not E. coli K88 faecal excretion, and
increased villus height in the small intestine (P<0.05). The number of enterocytes and goblet cells in the ileum
was not changed by formate supplementations. In saliva, total IgA activity tended to be reduced by acidifiers
(P--O.067). The anti-K88 IgA in the saliva, the blood and the jejunum secretion, and the expression of the
TNFet gene in the stomach and jejunum wall were not affected by the diet. The pH of the stomach, the
duodenum and the ileum was not affected while the formate reduced the pH in the colon and the caecum. Fatprotected calcium formate did not improve the results obtained with free formate supplementation. Calcium
formate has a growth-promoting effect in weanling pigs challenged with E. coli K88, independently of their
susceptibility to the intestinal adhesion of this strain. Its action seems to be more related to a general control
of the total E. coli rather than of E. coli K88. At the lame dietary concentration, no particular advantage comes
from using the protected formate instead of the free additive.
ID nr. 3706
Donati M, Di_Francesco A, Gennaro R, Benincasa M, Magnino° S, Pignanelli S, Shurdhi
A, Moroni A, Mazzoni° C, Merialdi° G, Baldelli R, Cevenini R
Sensitivity of Chlamydia suis to cathelicidin peptides
Vet Microbiol. - Vol. 123 ( 2007). - p 269-273. - 17 bib ref
Impact factor: 2,073
Nine Chlamydia suis isolates, obtained from pigs with conjunctivitis, were molecularly characterized by ompA
sequeneing and their in vitro susceptibility to six cathelicidin peptides (SMAP-29, BAC-7, 13MAP-27, BMAP27, BMAP-28, PG-1, LL-37) determined in celi culture. SMAP-29 was the most active peptide, reducing the
intracellular inclusion number by >50% at a concentration of 10 µg/ml (3 µM) in six of the nine isolates tested.
Three molecularly identical isolates were insensitive at a concentration as high as 80 µg/ml (25 µM). Of the
remaining cathelicidin peptides tested, BAC-7 and BMAP-27 were active against six C. suis isolates at a
concentration of 80 µg/ml (25 and 26 µM, respectively). Cathelicidins LL-37 and PG-1 did not show any antichlamydial activity at 80 µg/rnL.
ID nr. 3708
Trevisi P, Merialdi° G, Mazzoni M, Casini L, Tittarelli° C, De_Filippi S, Minieri L, Lalatta
_Costerbosa G, Bosi° P
Effect of dietary addition of thymol on growth, salivary and gastric function, immune response, and
Pag. 27 di 300 31/08/2011 excretion of Salmonella enterica serovar Typhimurium, in weaning pigs challenged with this microbe
strain
Ital J Anim Sci. - Vol. 6 suppl 1 ( 2007). - p 374-376. - 5 bib ref
Impact factor: 0,172
64 weaned pigs (24d-old), were fed a control diet or a control plus 1% thymol diet, with or without Salmonella
typhimurium challenge. Thymol reduced feed intake but did not affect significantly the fina] body weight alter
29d of trial. Salmonella did not affect the performances. Body temperature was lower in the thymol group
before, but also 1d after challenge, when Salmonella increased the values more in unsupplemented pigs.
Fecal excretion of S. typhimurium in challenged pigs was not changed by the diet. The thymol group had a
higher concentration of immunoglobulin (Ig) in serum before the challenge, but after there was an interaction
between diet and challenge. The diet and the challenge did not affect saliva parameters, and ATPase gene
expression in the fundic gastric mucosa. Thymol increased the number of parietal cells for 100 um depth of
oxintic gland. In conclusion the important supplementation with thymol reduced the intake, but not
performance in the medium period. Some factors of the gut barrier were positively influenced. Salmonella
challenge did not reduce significantly the performance of the weaned piglet.
ID nr. 3715
De_Jong JC, Smith DJ, Lapedes AS, Donatelli I, Campitelli L, Barigazzi° G, Van_Reeth K,
Jones TC, Rimmelzwaan GF, Osterhaus ADME, Fouchier AM
Antigenic and genetic evolution of swine influenza A (H3N2) viruses in Europe
J Virol. - Vol. 81 no 8 ( 2007). - p 4315-4322. - 29 bib ref
Impact factor: 5,341
Project PRC99007 - Epidemiological surveillance of influenza virus re-circulation in domestic and wild
animals: an investigation into antigenic characterization of circulating strains in susceptible domestic
animal species (pig, horse, avian species), intensification of surveillance in wild avian species and
from rural flocks, new immunizing strategies for swine - Barigazzi Dr. Giuseppe - Capofila MINSAN - 1999 - 347643000,00 LIT - 01/03/2001 0.00.00 - 28/08/2003 0.00.00 - 1 - N5 Parma - Foni Dr.ssa Emanuela - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
3 - N3 - Forlì - Massi Dr.ssa Paola - I
4 - ISS - Donatelli Dr.ssa Isabella - E
5 - INFS - De Marco Dr.ssa Maria Alessandra - E
6 - ISS 2 - Castrucci Dr.ssa Maria Rita - E
In che early 1970s, a human influenza A/Port Chalmers/1/73 (HRN2)-like virus colonized the European swine
population. Analyses of swine influenza A (HRN2)-viruses isolated in the Netherlands and Belgium revealed
that in che early 1990s, antigenic drift had occurred, away from A/1'ort Chalmers/1173, che strain commonly
used in influenza vaccines for pigs. Here we show that Italian swine influenza A (H3N2) viruses displayed
antigenic and genetic changes similar to those observed in Northern European viruses in che same period.
We used antigenic cartography methods for quantitative analyses of the antigenic evolution of European
swine H3N2 viruses and observed a clustered virus evolution as seen for human viruses. Although the
antigenic drift of swine and human H3N2viruses has followed distinct evolutionary paths, potential clusterdifferentiating amino acid substitutions in the influenza virus surface protein hemagglutinin (FIA) were in part
che smre. The antigenic evolution of swine viruses occurred A a rate approximately six times slower than che
rate in human viruses, even though che rates of genetic evolut on of che 11A at the nueleotide and amino
acid level vere, similar for human and swine H3N2 viruses. Continuous monitoring of antigenic changes is
recommended to give a first indication as to whether vaccine strains may need updating. Our data suggest
that humoral immunity in the population plays a smaller role in the evolutienary selection processes of swine
H3N2 viruses than in human H3N2 viruses.
ID nr. 3727
Pag. 28 di 300 31/08/2011 Berghoff J, Viezens J, Guptill L, Fabbi° F, Arvand M
Bartonella henselae exists as a mosaic of different genetic variants in the infected host
Microbiology - SGM. - Vol. 153 ( 2007). - p 2045-2051. - 33 bib ref
Impact factor: 3,110
Bartonella henselae is a fastidious bacterium associated with infections in humans and cats. The mechanisms
involved in the long-term survival of bartonellae despite vigorous host immune responses are poorly
understood. Generation of genetic variants is a possible strategy to circumvent the host specific immune
responses. The authors have recently demonstrated the coexistence of different genetic variants within the
progeny of three primary B. henselae isolates from Berlin by PFGE analysis. Aims of the present study were
to determine whether coexistence of different variants is a common feature of B. henselae isolates worldwide
and whether the genetic variants originally emerged in vivo. Thirty-four primary isolates from different
geographical regions were analysed by subjecting multiple single-colony-derived cultures to PFGE analysis.
Up to three genetic variants were detected within 20 (58.8%) isolates, indicating that most primary isolates
display a mosaic-like structure. The close relatedness of the genetic variants within an isolate was confirmed
by multi-locus sequence typing. In contrast to the primary isolates, no genetic variants were detected within
the progeny of 20 experimental clones generated in vitro from 20 primary isolates, suggesting that the variants
were not induced in vitro during the procedure of PFGE analysis. Hence, the genetic variants within a primary
isolate most likely originally emerged in vivo. Consideration of the mosaic structure of primary isolates is
essential when interpreting typing studies on B. henselae.
ID nr. 3728
Liu Z, Rank R, Kaltenboeck B, Magnino° S, Dean D, Burall L, Plaut RD, Read TD, Myers
G, Bavoil PM
Genomic plasticity of the rrn-nqrF intergenic segment in the Chlamydiaceae
J Bacteriol. - Vol. 189 no 5 ( 2007). - p 2128-2132. - 24 bib ref
Impact factor: 3,993
In ChIamydiaceae, the nucleotide sequence between the 5S rRNA gene and the gene for subunit F of the
Nattransloeating NADH-quinone reductase (nqrF or dmpP) has varied lengths and gene contents. We
analyzed this site in 45 ChIamydiaceae strains having diverse geographical and pathological origins and
including members of all nine species.
ID nr. 3736
Ferreri AJM, Dolcetti R, Magnino° S, Doglioni C, Cangi MG, Pecciarini L, Ghia P, Dagklis
A, Pasini E, Vicari° N, Dognini GP, Resti AG, Ponzoni M
A woman and her canary : a tale of chlamydiae and lymphomas
J Natl Cancer Inst. - Vol. 99 n 18 ( 2007). - p 1418-1419. - 7 bib ref
Impact factor: 15,271
ID nr. 3737
Ferrazzi V, Moreno Martin° A, Lelli° D, Gallazzi D, Grilli G
Microbiological and serological monitoring in hooded crow (Corvus corone cornix) in the Region
Lombardia, Italy
Ital J Anim Sci. - Vol. 6 no 3 ( 2007). - p 309-316. - 14 bib ref
Impact factor: 0,172
The health status of 276 hooded crows (Corvus corone corníx) from various provinces of Lombardy was
Pag. 29 di 300 31/08/2011 monitored for three years. Bacteriological examination detected E. coli (76%), Campylobacter jejuni (17%),
Salmonella typhimurium (11.6%), Yersinia spp. (6.5%), Clamydophila abortus and C. psittaci (2.6%); from six
birds showing severe prostration Pasteurella multocida
for Avian Influenza virus (AIV), West Nile virus (WNV) and only three samples were positive for Newcastle
disease virus (NDV) but only at serology.
ID nr. 3754
Circella E, Martella V, Lavazza° A, Lorusso E, Camarda A
Coronavirus associated with an enteric syndrome in a quail farm: genomic characterization of a viral
strain
Obiet Docum Vet. - Vol. 29 no 3 ( 2008). - p 5-8. - 19 bib ref
In this report, an enteric sindrome observed in a quail farm semi-intensively reared for hunting and restocking
is described. The affected birds displayed depression, dehydration, reduced growth and severe diarrhoea. A
coronavirus was detected by electron microscopy and RT-PCR in the intestinal content of the dead quail. The
virus was not cultivable using chicken embryos. By sequence analyses of a fragment 1b of the polymerase
gene, a 93% nucleotide identity of the quail coronavirus (QCoV) to the avian coronavirus (group 3) was
observed. The sequence analyses of the S1 fragment of the S gene showed a 16%-18% amino acid identity
of the QCoV wíth the infectious bronchitis virus and 79%-81% identity with turkey coronavirus (TCoV).
Actually, we are not sure whether the QCoV is a TCoV variant or a new avian coronavirus.
ID nr. 3755
Paoletti F, Luppi° A, Bosetti M, Di_Lecce R, Bonilauri° P, Cordioli° P
Clinical and pathological investigation in an outbreak of PMWS (Postweaning Multisystemic Wasting
Syndrome) and diagnostic methods comparison
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 495-503. - 15 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Samples from 20 swine lymph nodes (ln. inguinales superficiales) with PMWS clinical signs were submitted
for cytological, histological and real time PCR examination in order to establish a cytological grading. In this
comparative study the cases were collected in three groups with mild, moderate and severe lymphoid
microscopio lesions (group I, II, III respectively). High viral load was observed in all PMWS cases but the
higher amount of virus was found in samples collected in the group III (final case). Cytological examination
appeared in our experience as an important cornplementary diagnostic tool in the diagnosis of PMWS and
usefully to determine the grade of lesions.
ID nr. 3758
Sozzi° E, Luppi° A, Lelli° D, Moreno° A, Canelli° E, Cordioli° P, Brocchi° E, Bugnetti° M.
Molecular identification of Porcine teschovirus and enterovirus by RT-CR
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 459-466. - 18 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Project PRC2002007 - Improvement of SVD diagnosis: identification and neutralization of swine
enteroviruses affecting SVD virus isolation in tissue cultures. - Brocchi Dr.ssa Emiliana - Capofila MINSAN - 2002 - 208749,00 EUR - 15/04/2004 0.00.00 - 31/12/2006 0.00.00 - 1 - H1 - Reparto
di Biotecnologie - Brocchi Dr.ssa Emiliana - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
Pag. 30 di 300 31/08/2011 3 - IZSVE - Nardelli Dr. Stefano - E
4 - ISS - Tollis Dr.ssa Maria - E
First author Sozzi, Enrica
Porcine enterovirus (PEV) and teschovirus (PTV), belonging to the family Picornaviridae, are ubiquitous and
although the majority of infections are asymptomatic, they were related to polioencephalomyelitis, female
reproductive disorders, enteric disease and pneumonia. In the present study a total of 24 Italian porcine
entero-teschovirus isolates vere analysed by polymerase chain reaction. The assay tumed out to be a useful
diagnostic tool for molecular identification of porcine teschovirus/enterovirus strains and for the study of
molecular epidemiology and evolution of these viruses.
ID nr. 3759
Lombardi° G, Pavoni° E, Faita° R, Losio° MN, D'Abrosca° F, Luppi° A, Gelmetti° D,
Cordioli° P, Boni° P
SPF pig experimental infection with swine positive hepatitis E virus (HEV) faeces
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 451-458. - 8 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Project PRC2004008 - Preliminary assessment of the presence of hepatitis E infection in swine of
Lombardy, set-up of an ELISA serological method and PCR - Lombardi Dr. Guerino - Solo MINSAN - 2004 - 50000,00 EUR - 01/07/2005 0.00.00 - 30/06/2007 0.00.00 - 1 - G3 - Reparto
benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Lombardi Dr. Guerino - I
First author Lombardi, Guerino
Hepatitis E virus has recently been recognized as having zoonotie potential and could be transmitted from pig
to human. Pigs are identified as a potential animal reservoir and HEV is highly prevalent in the swine
population around the world. In this study the presence of HEV was investigateti in 17 pig farms of Northern
Italy (Lobardy), by RT-nested PCR on fresh stool samples. For the study 183 stool samples were collected
and 28 samples resulted positive to HEV. RT-nested PCR and genotyping demonstrated that all viruses were
closely related to different strains previously described and grouped iato the genotype 3. In the second part of
this study, Rt-nested PCR positive feces were used to prepare the HEV inoculum for the oral experimental
infections of four HEV-seronegative, three months SPF pigs. Other four, uninoculated pigs were housed in the
saure pen containing the four inoculateti pigs previously described. Two pigs, who vere not inoculateti, served
as negative control. The experimental design consisted of four oral inoculateti pigs, four uninoculated pigs
used as direct-contact controls and two uninoculated pigs used as negative control and housed separately.
Daily fecal swab and weekly blood sample, from each pig, avere tested for HEV RNA and anti-HEV antibodies
respectively.
ID nr. 3760
Lelli° D, Moreno° A.Sozzi° E, Luppi° A, Canelli° E, Brocchi° E, Cordioli° P
Swine influenza viruses typing with monoclonal antybodies
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 445-450. - 6 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Project RELO2005910SUINFL - Diagnostic and control strategies for respiratory diseases of swine, with
emphasis on influenza virus infection and the epidemiological role of pigs with respect to avian
influenza virus strains. - Alborali Dr. Loris - Capofila - REGIONE - 2005 - 65000,00 EUR 01/05/2005 0.00.00 - 01/03/2009 0.00.00 - 1 - M1 - Brescia - Alborali Dr. Loris - I
2 - ARAL - Crema - Battaglia Dr. Massimo - E
First author Lelli, Davide
Pag. 31 di 300 31/08/2011 Monoclonal antibodies specific for H1, H3, N1, N2 have been produced, characterized and used to develop
sandwich-ELISA. This ELISA test was used to type 37 swine influenza viruses belonged to 14 H1N1, 16
H3N2 and 7 H1N2 subtypes. All the HINI strains, except one, exhibited strong reactivity in the H1-ELISA and
NI-ELISA (Mabs 2C2 and 5B2 respectively) but, in contrast, only a H1N2 strain resulted positive in the H 1ELISA. The H1-ELISA, N1-ELISA and N2-ELISA developed, showed high specificity and sensitivity, enabled
the identification of H1N1 and H3N2 swine influenza virus strains. In contrast, these tests showed a very low
sensitivity in the identification of H1N2 strains. The H1N1 strain (5433/01) that reacted negative in the H1ELISA, showed a strong genetic relationship regarding the HA gene of H1N2 viruses. In contrast, the only
H1N2 strain typed by the Hl-ELISA, showed a HA gene sequence closed related with the H1N1 HA genes, as
described in a previously work.
ID nr. 3761
Castellan A, Alborali° L, Zanoni° MG
Intensive vaccination strategy against porcine Circovirus type2 (PCV2) in the control of a PCV2
disease (PCVD) outbreak in a farrow-to-weaning farm
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 419-423. - 3 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Alborali, Giovanni Loris
A diagnostic protocol was applied in an Italian farrow-to-weaning farr 1500 sows after the appearance of
severe mortality and wasting in the weaners. A complicated outbreak of PCVD (Circovirus type 2 diseases)
was identified. The effects an intensive vaccination with CIRCOVAC° (inactivated vaccine against PCV2) were
studied. The vaccination was able to reduce dramatically mortality, the number of poors doers and the use of
antibiotics; PDNS completely disappeared and growth performances returned back to optimal level.
ID nr. 3762
Sarli G, Ostanello F, Morandi F, Fusa L, Bacci B, Nigrelli° A, Alborali° L, Dottori° M,
Vezzoli° F, Barigazzi° G, Fiorentini° L, Sala V, Leotti G
Diagnosis of PMWS in Italy : results of 18 months of activity
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 401-410. - 16 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Nigrelli, Arrigo Daniele
In 18 months of activity our team has tested 64 farms (altogether 229 pigs) for PMWS. PMWS was diagnosed
on 48 farms, in 21 of which the total number of the examined subjects had lesions of PMWS, whereas in the
remaining 27 only some animals showed PMWS. The protocol applied is based on the combination of the
typical histological lesion in lymphoid and long tissues with the immunohistochemical assessment of PCV2.
The test revealed stronger when applied to a group of animals than on a single pig.
ID nr. 3763
Appino S, Alborali° L, Pregel P, Guarda F
Mycoplasma spp. in arthritis in swine
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 389-392 - 8 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Pag. 32 di 300 31/08/2011 Terme (PR) : 13-14 marzo 2008)
Porty-one synovial fluids have been collected from regularly slaughtered swine clinically affected by sierositis,
arthritis - polyarthritis, in order to evaluate the presence of Mycoplasma spp. Macroscopical lesion were
characterised by sub-acute to acute sierositis associated to an increase of synovial fluid and fibrin, but without
signs of superficial erosions. A rnild proliferation of synovial membrane was present.
ID nr. 3764
Calzolari° M, Leotti G, Bianchi M, Sarli G, Dottori° M
Pseudo-myiasis caused by Ophira Aenescens (Diptera: muscidae) in swines
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 361-366 - 16 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Calzolari, Mattia
In March 2007 we analyzed pig's stool come from a farro in Imola (Bologna) containing Diptera larvae and we
suspected that phenomenon could be an unusual myiasi. Our visit in the piggery confirmed the abundant
presene of these larvae only in the room where the event was shown and the necroscopy on one of the .pigs
showed the presence of the saure larvae into the digestive system. The laboratory identification of all sampled
larvae confirmed that they were all the lame species, Ophyra aenescens. We explain the observed
phenomenon by the pigs' ingestion of larvae in the environment, that were not digested and then discharged
by the stool, originating a situatuation similar to a myiasi (called pseudo-miasi).
ID nr. 3765
Merialdi° G, Bonilauri° P, Dottori° M, Nigrelli° A, Martelli P
Monitoring respitory disease at slaughterhouse using lung and pleural lesions score and serology
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 319-325 - 13 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Merialdi, Giuseppe
Monitoring of lung lesions at slaughterhouse is an efficient tool to estimate the prevalence and severity of
respiratory disease. Different methods have been described for the evaluation of Enzootic Pneumonia (EP) or
lesions due to Actinobacillus pleuropneumotziae (App) at slaughterhouse. Lung and pleural lesions scoring in
combination with serological data can provide a picture of the impact of respiratory disease at herd level. The
results at slaughterhouse on 46 batches from different herds showed lesions referred to EP and to Chronic
Pleuritis (CP) respective in 46.3 and 49.0% of the examined lungs. Among the respiratory pathogens, PRRSV
and App revealed a 100% seroprevalence.
ID nr. 3767
Rosetti M, Frasnelli° M, Fabbri F, Arienti C, Vannini I, Tesei A, Zoli W, Conti M
Pro-apoptotic activity of cyclopentenone in cancer cells
Anticancer Res. - Vol. 28 ( 2008). - p 315-320. - 22 bib ref
Impact factor: 1,414
Studies on cyclopentenone prostaglandins (CPPGs), clavulones and other cyclopentenones have shown that
these compounds have a significant anticancer activity mediated by their cyclopentenone (CP) chemical
Pag. 33 di 300 31/08/2011 moiety. In this study the cytotoxicity against cancer cells of the model compound cyclopent-2-en-I-one (2CP)
was investigateti. Being a highly water soluble small molecule, 2CP could be an ideal candidate to overcome
pharmacological issues related to drug delivery and penetration. Its cytotoxic activity was tested on various
melanoma and lung cancer cells. Interestingly, 2CP was both cytotoxic and pro-apoptotic, more pronounced
on melanoma cells, at concentrations in the submicromolar range. On melanoma cells its mechanism of
action was mediated by the mitochondria and the activation of caspase 3.
ID nr. 3768
Terreni M, Merialdi° G, Bonilauri° P, Bonci° M, Tittarelli° C
Longitudinal serological study on the natural infection from Lawsonia intracellularis, in different
swine production system
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 301-310. -11 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Ileitis is a worldwide disease responsible of relevant economic losses. Different diagnostic test are available
for evaluating the presence and the diffusion pattern of Lawsonia intracellularis in farm. Objective of the study
was to determine the presence and the evolution of seroprevalence, trough and ELISA test, in dfferent
production systems. At the same time, has been evaluated the relationship between the presence of
antibiotics used for strategic medication and infection. The seroprevalence increase progressively, from 60
days of age (18%) to 210 (78%). The production system impact on the beginning of the infection, that was
earlier in one site vs multisite. Antibiotics didn't prevent infection and produced subpopulation of infected pigs
as well as negative, receptive pigs..
ID nr. 3769
Rota_Nodari S, Candotti° P, Nassuato C, Ferro P
Field trial to evaluate the efficacy of a feed medication program for sows with tylosin and
sulphametazin (Tylan®Sulfa G100 premix) during pre-farrow and lactation periods
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 271-274. - 3 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Rota_Nodari, Sara
The study was performed in a farrow to weaning farm with 130 sows managed by a batch farrowing system in
Northern Italy, with signs of rhinitis in a few fat pigs. The study included 6 consecutive groups of sows
alternately assigned to either the control group (n=100) or the treatment group (n=101). During the 7 days
preceding farrowing and throughout the lactation period (27 days), the sows of the treatment group (n=101)
were given feed medication with 100 g/100 kg Tylan® Sulfa G100 Premix. The prophylactic treatment of the
sows with Tylan 12 Sulfa GI00 Premix resulted positive effects on the litter, in terms of both the number of
weaned piglets (10,43 vs 9,87) and weight at weaning (7,89 kg vs 7,29 kg). Treatment produced also positive
effects on the sows, showing it possible to cut the costs of additional health treatment and, above all, showing
an increase in the number of piglets born alive at the next delivery , that is 9,8 in the treatment group and 8,8
in the control group.
ID nr. 3770
Ferro P, Tonon F, Bonilauri° P
Evaluation of two different in feed medication therapeutic programs on attrition as a consequence of
swine respiratory disease in nursery
Pag. 34 di 300 31/08/2011 Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 267-270. - 3 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
After diagnosis of respiratory disease in the nursery sector of the farro where the study was performed, two
groups of 110 pigs per group (PulmotilTM Group and Control Group) were treated with two different in feed
medication therapeutic programs for the first 21 days after the weaning. The impact of the two different
treatments on Attrition (culls, deaths and lightweights) was evaluated from weaning to the market place. The
data about culls and deaths were recorded, all the pigs were individually weighted at 3 and 10 weeks of age,
around 25-30 % of each group was weighted at 20 weeks of age, then the individua) carcass weight was
collected. PulmotilTM Group showed mean weights higher than Control Group at 10 and 20 weeks of age,
and also the mean carcass weights was higher in the PulmotilTM Group than in the Control Group. In
conclusion the authors point out as the use of PulmotilTM, in this study, increased ammal health, with better
performances from weaning to the market piace and produced more Full Value PigsTM.
ID nr. 3771
Candotti° P, Dotti° S, Guana S, Rota_Nodari S, Amadori° M, Villa° R, Petrini S, Lombardi°
G, Ferrari° M
Susceptibility of pure bred large white and landrace pigs to experimental infection with PRRSV
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 253-256 - 3 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Project PRC2002013 - Susceptibility of different pig breeds to PRRS - Candotti Dr. Paolo - Capofila MINSAN - 2002 - 139810,29 EUR - 15/02/2005 0.00.00 - 14/02/2009 0.00.00 - 1 - G3 - Reparto
benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Candotti Dr. Paolo - I
2 - CERSA - Giuffra Dr.ssa Elisabetta - E
3 - UNI MI - Domeneghini Dr.ssa Cinzia - E
4 - IBBA CNR - Boettcher Dr. Paul - E
First author Candotti, Paolo
In this study, twenty pigs, thirty day old of two different pure bred lines: fourteen Large White and fourteen
Landrace, were experimentally infected with PRRS virus. The animals were investigated for thirty days by
clinical, virological and à immunological tests, to compare the infection response differences between two pig
lines. The collected data seem to evidence a more severe clinical sign in Large White aniimals than in
Landrace and tris finding could be in relationship with different values of IL-10, IFI –(ypsilon) SC and IgM, that
were detected in the two pure bred lines.
ID nr. 3772
Sozzi° E, Luppi° A, Lelli° D, Moreno° A, Canelli° E, Cordioli° P, Lavazza° A, Brocchi° E
Development and application of a PED coronavirus antigen detection enzyme-linked immunosorbent
assay
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 247-252. - 9 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Sozzi, Enrica
Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. This
paper describes a monoclonal antibodies based enzymelinked immunosorbent assay (ELISA) development
for the detection of porcine epidemic diarrhoea virus (PEDV) in fecal samples. This work demonstrates the
value of the ELISA technique for detecting PEDV on field samples, in a comparative study with RT-PCR.
Pag. 35 di 300 31/08/2011 Fecal samples were collected from pig farms located in Pianura Padana during 2006-2007.
ID nr. 3773
Petrini S, Gavaudan S, Barocci S, Briscolini S, Sebastiani C, Mancini R, Paniccia P, Villa°
R, Ferrari° M
Isolation and characterization of porcine circovirus type 2 (PCV2) in wild boars in central Italy
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 239-245. - 8 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Porcine circovirus type 2 (PCV2) was first identified in domestic swine. Most studies have indicated that PCV2
infects only swine but there is little information on porcids other than improved breeds of domestic swine. We
show that PCV2 was identified in wild boars by gross and/or histological examinations, isolation, PCR and
Real-Time PCR. The ORF2 of the isolated PCV2 had 95-97% homology with ORF2 of reference PCV2
isolates. These diagnostic data suggest that PCV2 can infect and cause disease in Sus scrofa subspecies
other than swine.
ID nr. 3774
Morandi F, Ostanello F, Fusaro L, Bacci B, Nigrelli° A, Alborali° L, Dottori° M, Vezzoli° F,
Barigazzi° G, Fiorentini° L, Sala V, Leotti G, Sarli G
Immunohistochemical investigation on swine proliferative and necrotizing pneumonia (PNP) in Italy :
preliminar results
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 215-222. - 16 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Proliferative and necrotizing pneumonia (PNP) is a particular form of interstitial pneumonia in weaning and
post-weaning pigs characterized by hypertrophy and hyperplasia of type II pneurnocytes and presence of
coagulative necrosis and granular debris within alveolar spaces. American and European studies suggest
PRRSV and PCV2 as the probable causes of tbc disease. An immunohistochemical study was carried out on
24 PNP lungs to evaluate the role of PRRSV and PCV2 in PNP lesions in Italy. Results vere as follows: coinfection (PCV2 and PRRSV) in 7 PNP lungs, PRRSV only in 9, PCV2 only in 4, and neither of the two
antigens was found in the remaining 4. These findings support the major role of PRRSV as cause of PNP in
Italy, even if co-infection is characterized by more severe histological aspects.
ID nr. 3775
Spaggiari° B, Gherpelli° Y, Bonilauri° P, Merialdi° G
Development and preliminary application of a method for in vitro susceptibility testing of bacterial
pathogens of swine origin versus essential oils
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 209-214. - 21 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Spaggiari, Brunella
A method is presented for testing in vitro susceptibility of different strains of 2 swine pathogens, Salmonella
Typhimurium and Pasteurella inadtocidci, to carvacrol, a major component of oregano (Origanuni vulgare),
thyme (Thyrnus vulgaris) and other plani essential oils. Carvacrol antimicrobial activity was evaluated by
Pag. 36 di 300 31/08/2011 means of the minimum corncentration inhibiting bacterial growth (MIC). Ori all tested strains of P. nitiltocida
carvacrol MIC was 50,0625%, while on tested strains of Salmonella Typhimurium carvacrol MIC varied from
0,25%, to 0,125%. These results confirm the marked in vitro antimicrobial activity of carvacrol..
ID nr. 3776
Bano L, Drigo I, Agnoletti F, Cocchi M, Ferro T, Bacchin C, Guolo A, Marcon B, Bonci° M,
Merialdi° G
Evaluation of bacteriological examination, ELISA toxins test and real-time PCR in the diagnosis of
porcine Clostridium difficile associated disease
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 201-208. - 19 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
In order to investigate the cole of Clostridium difficile (CD) in swine enteritis outbrealcs, 79 faecal Samples, 30
intestinal contents and 12 rectal swabs were collected from subjects with a history of diarrhoea, in 31 different
farms. Samples were stratified by growing phase (suckling, post-weaning, growing, fattening). Each sample
was cultured in a selettive medium for CD. Isolates were identified by a cornmercial biochemical panel kit and
by the specific CD Ihi gene PCR. Each isolate was tested by multiplex PCR to reveal the presente of tcdA and
tcdB genes encoding for toxin A and toxin B respectively. The samples were screened for CD toxins A and B
by using a commercia) ELISA. 26 intestina) contents and 73 faecal samples were tested by Real-Time PCR to
enumerate CD CFU per g of sample. CD was recovered from 27 samples and the higher prevalente was
evidenced in suckling pigs (43.5/0). Thirteen strains resulted positive for botti tcdA and tcdB genes, one strain
was tcdA-/tcdB-, whereas 13 resulted tedA-/tcdB+. 28 samples tested positive by Real-Time PCR and the
higher concentrations of CD (10(Allaseconda)-10(allaterza) UFC per g) was detected in samples resulted
positive for botti toxins and cultural examinations. Toxin A and B were detected in 21 of the 27 samples
positive for CD. This study point out the fact that CD is involved in outbreaks of enteric disease in swine,
irrespective of age even if the higher prevalente was detected in suckling pigs. Furtherinore the enumeration
of this enteric pathogen by Real-Time PCR coupled with ELISA toxin test provides a rapid and accurate tool
for the diagnosis of clostridiosis supported by CD.
ID nr. 3777
Nigrelli° AD, Alborali° L, Boldini° M, Fabbi° M, Vezzoli° E
Salmonella enterica infection spreading in Northern Italy herds : subdivision model into the groups
with different prevalence
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 195-200. - 11 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
Project PRC2005015 - Preliminary study for the development of a model for the surveillance of Salmonella
enterica infection in fattened pigs and for the assessment of prevalence decrease over time - Nigrelli
Dr. Arrigo - Solo - MINSAN - 2005 - 139780,00 EUR - 01/08/2006 0.00.00 - 30/09/2008 0.00.00 1 - M6 - Mantova - Nigrelli Dr. Arrigo - I
2 - M4 - Cremona - Boldini Dr. Massimo - I
3 - M5 - Lodi - Luini Dr. Mario - I
4 - M8 - Pavia - Fabbi Dr. Massimo - I
5 - M1 - Brescia - Alborali Dr. Loris - I
First author Nigrelli, Arrigo Daniele
The authors found, by two different Elisa-tests, an high spreading of Salmonella enterica infection in 109 pig
hends of Northern Italy, with different characteristics (size, floor, type of production) either into the sows or into
the fattening pigs. The Authors found, too, the possibility of subdividing herds into groups with different
infection level, for measuring progressive reduction of Salmonella enterica spreceding the Authors didn't found
Pag. 37 di 300 31/08/2011 an positive correlations between positive sera percentage and size, and floor or production type of herds
tested. Longitudinal serologic studies confirmed the high seroprevalence in sows and fattening pigs.
ID nr. 3778
Spaggiari° B, Gherpelli M, Magnino° S, Fallacara° F, Bonilauri° P, Dottori° M, Merialdi° G
Case report of reproductive disorder associated with Chlamydophila abortus in swine
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 189-194. - 19 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
First author Spaggiari, Brunella
The Authors report a case of reproductive disorder characterized by low fertility associated to vaginal
discharges in a swine farrow-to-wean herd. Sows were mated with the semen of farm boars, some of which
suffered from orchitis. After culling of sows with chronic reproductive failure and sampling of genital apparatus
at slaughter, gross pathology bacteriology and biomolecular investigations were performed on reproductive
organs. Semen samples from 6 farm boars were also tested. Chlamydophila abortus was evidenced from uteri
in association with other bacterial agents. Chlamydophila spp. was isolated from boar semen. After an in feed
medication with chlortetracycline fertility was restored to herd standard values.
ID nr. 3779
Mazzoni C, Bonilauri° P, Borri E, Tonon F, Ponzio M
Farrowing room efficiency improvement in a herd with two-week batch farrowing system : a field
experience
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 177-187. - 12 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
This paper reports a field experience on a farm that achieved a considerable farrowing room efficiency
improvement using a methodical work of farrowing synchronization and assistance.
ID nr. 3780
Alborali° L, Abrami S, Zanardi° G, Avisani° D, Bellini° S
Swine vescicular disease and bio-security measures : risk factors in 2006-2007 outbreaks in
Lombardia
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 115-124. - 4 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) Tavola rotonda "La
malattia vescicolare del suino : attualita' e prospettive in seguito all'emergenza 2006-2007" (34. :
Salsomaggiore Terme (PR) : 13-14 marzo 2008)
First author Alborali, Giovanni Loris
Authors describe the epidemiological features of the SVD outbreaks occurred in Lombardia during the 2006
epidemic, the main attention is focalized on the outbreaks of Brescia and Mantova provinces. The 2006 2007
epidemic were characterized by high diffusion of virus between herds. This is related to high concentration of
swine herds in protection area where farms from 73 became 121 during period 1996-2007. In the second
period of epidemic the reduce of area between herds were the main risk factor for introduction of SVD in 76
%of outbreaks. For the purpose of this paper the following information are taken into account: herds evolution
in protection area, risk factors for the introduction and spread of the infection, weakness of the bio security
Pag. 38 di 300 31/08/2011 measures applied. On the basis of the risk factorshighlighted in the outbreaks (movement of pigs, introduction
of contmined haulier's vehicles for different purpose, proximity to other outbreaks, use of manure on
agricoltural land fertilizer) bio security measures were realized to minimize the risk of SVD introduction and
spread..
ID nr. 3781
Abrami S, Covi M, Cominardi P, Loris_Alborali° GL
Outbreaks management : perspectives and critical points
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 103-113. - 8 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) Tavola rotonda "La
malattia vescicolare del suino : attualita' e prospettive in seguito all'emergenza 2006-2007" (34. :
Salsomaggiore Terme (PR) : 13-14 marzo 2008)
The legislative reference is the Decree of the President of the Republic 17 May 1996 n 362, implementation of
the UE/119/92 laying down general measures for the control of certain animal diseases as well as some
specific measures for the Swine Vesicular Disease (SWD) and the Emergency Plan/ Operative Manual
drafted by the Vesicular Diseases Reference Centre of Brescia (CERVES). Measures adopted in protection
and surveillance areas are complex, strict and onerous also from an administrative point of view. The situation
becomes more critical as more new outbreaks of SVD break down and then time needed for controls and
measures revocation gets greater. The management of the serological and clinical controls in the herds
should be especially accurate. Particularly important is the informative flow both for Municipalities, Region and
Ministry and for farmers, their Associations and the media. In order to eradicate outbreaks as quickly as
possible should be available an operative protocol aimed to guarantee: that the spreading of the virus is as
less as possible, the safety and health of workers, the animals protection during culling operations. Culling
operations are complex and in case of big sized herds can lead to high contamination level of the
environment, eventual risk source in high animal density areas. Other elements are cleaning and disinfections
of the herds, proper sanitation of sewage and introduction in the herds of a correct number of guard animals.
The last action, sensitive and onerous both administratively and technically, is the iter to get the indemnity for
farmers.
ID nr. 3782
Zanardi° G, Alborali° L, Bellini° S, Avisani° D
Swine vesicular disease in Lombardy Region : comparison among the epidemics ocurred from 1998
to 2007
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 91-102. - 8 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) Tavola rotonda "La
malattia vescicolare del suino : attualita' e prospettive in seguito all'emergenza 2006-2007" (34. :
Salsomaggiore Terme (PR) : 13-14 marzo 2008)
First author Zanardi, Giorgio
In the last decade four epidemics of swine vesicular disease (SVD) occurred in Lombardy Region. The first
one started at the end of October 1998 and finished at the end of February 1999. Eighteen outbreaks were
notified in four provinces: 7 in Cremona, 6 in Mantova, 3 in Brescia and 2 in Bergamo. Following this epidemic
Veterinary Service of Lombardy Region carried out all extraordinary plan aimed at eradicating the disease and
achieve the free status from SVD. The second incursion of SVD virus occurred in the first half of 2000,
involving three dealers, two in the province of Mantova, directly linked, and one in the province of Brescia. All
the outbreaks were considered primary. In the third epidemic in 2002, during the routine surveillance activities,
SVD was identified simultaneously in a breeding farm and in a dealer's premises of the Bergamo province.
The epidemiological investigations in these two primary outbreaks allowed for the identification of 11
secondary outbreaks, 9 in Lombardy (all in Bergamo province) and 2 in the bordering Veneto and Emilia
Romagna regions. All the secondary outbreaks were a cul de sac for the infection; in fact they did not give rise
Pag. 39 di 300 31/08/2011 to secondary outbreaks. During the latest epidemic, started at November 2006 and finished at October 2007,
53 outbreaks were notified and about 148.464 animals were stamped out. Brescia was the province more
involved in the spreading of the disease, with 32 outbreaks and the culling of 100.591 herds. No novelty was
available on the mode of the disease transmission intra and inter-herds during the latest SVD epidemic. The
high number of outbreaks and the restriction of the animal movement, due to the persistence of the SVD virus
in a densely populated area. caused huge economic losses. This speech is aimed at comparing the
characteristics of the different epidemics occurred in Lombardy Region in the light of the change of swine
husbandry. The epidemiological remarks show the critical points about the prevention and the management of
SVD in densely populated areas.
ID nr. 3783
Bellini° S
Swine vesicular disease in Italy : epidemiological situation and future control strategies
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 83-89. - 1 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) Tavola rotonda "La
malattia vescicolare del suino : attualita' e prospettive in seguito all'emergenza 2006-2007" (34. :
Salsomaggiore Terme (PR) : 13-14 marzo 2008)
First author Bellini, Silvia
SVD has been persistently reported in Italy in the last decade, hence surveillance and eradication activities
are in place. During 2006 - 2007 a recrudescenze of SVD was reported in Italy and outbreaks were identified
in Northern regions, Lombardia resulted the one mainly affected. The presence of the disease in Northern
Regions caused heavy economic losses in swine farming and meat processing industry. Surveillance and
eradication activities for SVD have to be maintained in Italy to reach the disease free status for the whole
Country. Anyhow, surveillance activities are all essential for trade purpose, in fact they are necessary to
demonstrate that part of Country is already SVD-free. The presence of a disease, such as SVD, impairs the
critical factors highlighted during the 2006 - 2007 epidemic have been taken into account to review the
surveillance actions foreseen in the national surveillance and eradication plan.
ID nr. 3785
Dottori° M
Helminthiasis in swine
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 23-27. - 15 bib ref
Giornata di studio "Endoparassitosi nella filiera produttiva del suino" : Marmirolo (MN) : 12 ottobre 2007)
First author Dottori, Michele
The most important helminthiasis of swine are reviewed, with focus of those who are more prevalent in Italian
herds. The biological cycle, the epidemiology and the sanitary and economie impact on swine herds are
considered.
ID nr. 3786
Dekker A, Sammin D, Greiner M, Bergmann I, Paton D, Grazioli° S, De_Clercq K,
Brocchi° E
Use of continuous results to compare ELISAs for the detection of antibodies to non-structural
proteins of foot-and-mouth disease virus
Vaccine. - Vol. 26 ( 2008). - p 2723-2732. - 10 bib ref
Impact factor: 3,377
Pag. 40 di 300 31/08/2011 Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
Six tests for detection of antibodies against the non-structural proteíns. of foot and-mouth disease virus
(FMDV) were compared at an international workshop in Brescia, Italy in 2004 on the basis of dichotomous test
results. However, as results from att of these assays were also available on a continuous scale, validation
was extended by calculatìng and subsequent[y analysing the receiver-operator characteristic (ROC) curves
and likelihood ratios (LR) for each test method. For the purposes of these analyses, test results for a total of
1337 sera were selected from the Brescia workshop dataset, 237 sera that had been obtained from cattle
exposed to FMDV and 1100 sera obtained from cattle that were not exposed to the virus; sera from "exposed"
cattle were considered to be "true positives" and sera from "non exposed" cattle were considered to be "true
negatives". Analysis of ROC curves showed that at specificities of both 99 and 99.5%, the IZS-Brescia and
the Ceditest ELISA had significantly better detection rates in exposed cattle than the other ELISAs. The ROC
analysis confirms the previous finding that the IZS-Brescia and the Ceditest ELISAs have both better
detection rates in exposed cattle combined with a high specificity. The analysis of likelihood ratios provides
information that may be very useful in the interpretation of test results, and a working example is presented to
show how these likelihood ratios might be used in an objective approach to deciding the true infection status
of surveyed populations.
ID nr. 3788
Terzano C, Gamba° V, Moretti S, Galarini R, Dusi° G
Development and validation of a confirmatory method for the determination of sulphonamides in
milk by HPLC with diode array detection
Residues of veterinary drugs in food : proceeding of the Euroresidue VI conference Egmond aan Zee, The
Netherlands, 19-21 May, 2008 / edited by L.A. van Ginkel, A.A. Bergwerff organised under the auspices of
the Federation of European Chemical Societies ( FECS ). Division of Food Chemistry. - [Bilthoven : National
Institute of Public Health and the Enviroment (RIVM), Laboratorium for Residue Analysis, 2008, 2008]. - p
223-227 - 6 bib ref
Euroresidue : conference on residues of veterinary drugs in food (6th : Egmond aan Zee (Netherlands) :
19-21 May, 2008)
A simple multiresidue method for the determination of 7 sulphonamides residues (sulfadiazine, sulfapyridine,
sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine and sulfaquinoxaline) in milk samples
was developed and validated. The drugs were extracted with a mixture chloroform/acetone and simply
cleaned up on a action exchange solid phase extraction column. The analytes' determination was carried out
using HPLC with UV-DAD detection at 270 nm. The procedure was validated as a quantitative confirmatory
method according to the EU Decision 2002/657/EC. The developed method shows good linearity, specificity,
precision, ruggedness and is able to confirm each sulphonamide residue above 20 pg kg-1. Decision limits
around 110 µg kg-1 and recovery above 60% were obtained for all the analytes. The results of the validation
process demonstrate that the method is suitable for application, as confirmatory method, in European Union
statutory veterinary drug residue surveillance programmes.
ID nr. 3789
Dusi° G, Bozzoni° E, Assini° W, Gasparini° M, Ferretti° E
Development and validation of a confirmatory method for the determination of resorcyclic acid
lactones in urine sample by liquid chromatography-tandem mass spectometry
Residues of veterinary drugs in food : proceeding of the Euroresidue VI conference Egmond aan Zee, the
Netherlands, 19-21 May, 2008 / [Bilthoven : National Institute of Public Health and the Enviroment (RIVM),
Laboratorium for Residue Analysis, 2008, 2008]. - p 235-240 - 7 bib ref
Pag. 41 di 300 31/08/2011 Euroresidue : conference on residues of veterinary drugs in food (6th : Egmond aan Zee (Netherlands) :
19-21 May, 2008)
First author Dusi, Guglielmo
The determination of zeranol, its metabolites taleranol and zearalanone is complicated by the occurrence of
other resorcylic acid lactones (zearalenone, a- and ß-zearalenol). The aim of this study is a very quickly
analytical procedure for the determination of all six resorcylic acid lactones. The urine sample was subjected
to an enzymatic deconjugation and then simply cleaned up on an immunoaffinity column. The analytes were
detected by liquid chromatography-negative ion electrospray tandem mass spectrometry using deuteriumlabelled internal standards. The method was validated as a quantitative confirmatory method according to EU
Decision 2002/657/EC. The results obtained show good linearity, accuracy and ruggedness. The decision
limits obtained were around 0.6 µg L-1 for all the analytes.
ID nr. 3790
Galarini R, Fioroni L, Dusi° G
About the decision limit for permitted substances
Residues of veterinary drugs in food : proceeding of the Euroresidue VI conference Egmond aan Zee, the
Netherlands, 19-21 May, 2008 / [Bilthoven : National Institute of Public Health and the Enviroment (RIVM),
Laboratorium for Residue Analysis, 2008, 2008]. - p 271-274 - 11 bib ref
Euroresidue : conference on residues of veterinary drugs in food (6th : Egmond aan Zee (Netherlands) :
19-21 May, 2008)
This paper describes the problems came out in the practical application of the decision limit (CCa) in the
analysis of substances with an established maximum residue limit (MRL). For confirmatory methods, CCa is a
fundamental performance characteristic introduced by EU Decision 2002/657/EC. Since the decision limit
takes automatically into account the measurement uncertainty, for permitted compounds, it lies always above
the MRL. However, in some cases the permitted limit is fixes as a “sum MRL” (e.g. sulfonamides or
tetracyclines) and this implies that, if in a sample two or more of the regulated molecules are found, the
analyst should face the lack of criteria for sample judgement (compliance or not). In these particular
circumstances, the uncertainty approach introduced by ISO, opportunely simplified taking into account only
the intra-lab reproducibility sources of variability, could better help to investigate the samples compliance.
Moreover the use of uncertainty estimation unify the criteria for samples judgment with other analytical fields
(e.g. environmental contaminants) and avoids the misunderstandings generated by the use of the same term
“decision limit” for both banned and permitted substances.
ID nr. 3792
Luppi° A, Losio° MN, Lombardi° G, Faita° R, Pavoni° E, Cordioli° P, Boni° P
Hepatis E virus in humans and animals
Osservatorio. - Vol. 11 no 3 ( 2008). - p 4-7
First author Luppi, Andrea
Hepatitis E virus (HEV) is an emerging pathogen belonging to a newly recognized family of RNA viruses
(Hepeviridae). HEV is an important enterically transmitted human pathogen with a worldwide distribution. It
can cause sporadic cases as well as large epidemics of acute hepatitis. Epidemics are primarily waterborne in
areas where water supplies are contaminated with HEV of human origin. There is increasing evidence,
however, that many animal species are infected with an antigenically similar virus. A recently isolated swine
virus is the best candidate for causing a zoonotic form of hepatitis E. The virus is serologically cross-reactive
with human HEV and genetically very similar, and the human and swine strains seem to be cross-infective.
Very recent evidence has also shown that swine HEV, and possibly a deer strain of HEV, can be transmitted
to humans by consumption of contaminated meat. In this review, we discuss the prevalence, pathogenicity,
diagnosis and control of human HEV, swine HEV, the related avian HEV and HEV in other hosts and potential
Pag. 42 di 300 31/08/2011 reservoirs.
ID nr. 3795
Scordella G, Bresolin R, Rubini° S, Fedrizzi° G
Food safety and quality requirements for importing aquaculture products in European Community :
present and future
World Aquaculture 2008 : May 19-23, 2008 Busan, Korea / World Aquaculture Society. - [s.l. : s.n, 2008]. - p
643
World Aquaculture : Busan, Korea : May 19-23, 2008)
The European Union is collectively the largest importer of seafood in the world, with individual member states
making up five of the world’s seven largest edible seafood importers. In 2002, the EU member states imported
edible seafood valued at €24.4 billion. Over the past decade total EU seafood imports have almost doubled in
value. The import rules for finfish and shellfish are harmonised, meaning that the same rules apply in all EU
countries. The European Commission’s Directorate General for Health and Consumer Protection (DG
SANCO) receives and distributes
notifications to the food and feed inspection services of member state, as well as to other countries. Its rules
for fish and shellfish products seek to guarantee that all imports fulfil the same standards as products from the
EU Member States. The rather strict food safety rules and regulations in EU is a direct consequence of recent
food scandals (BSE, Dioxin problem, Salmonella, Listeria and Cholera scares) that entered the minds of EU
consumers as potential threats. Furthermore, in the past years, the number of alerts and notifications from the
EU Rapid Alert and Safety for Food and Feed (RASFF) has risen significantly (from 698, in 1999, to 3024, in
2002). Imports of fishery products into the EU are subject to official certification, based on the recognition of
the competent authority of the non-EU country by the European Commission. Public authorities must ensure
credible inspections and controls throughout the production chain, which cover all relevant aspects of hygiene,
public health and also animal health. The eligibility criteria are:
1. Exporting countries must have a competent authority which is responsible for official controls throughout
the production chain.
2. Live fish, their eggs and gametes intended for breeding and live bivalve molluscs must fulfil the relevant
animal health standards.
3. The national authorities must also guarantee that the relevant hygiene and public health requirements are
met.
4. A control plan on heavy metals, contaminants, residues of pesticides and veterinary drugs must be in place
to verify compliance with EU requirements.
5. Imports are only authorised from approved establishments, inspected by the competent authority of the
exporting country and meet EU requirements.
6. Inspections by the Commission’s Food and Veterinary Office are necessary to confirm compliance with the
above requirements.
Because of high expectations regarding the safety and quality of their food, the food law of the EU implements
the principle of quality management and process-oriented controls throughout the food chain - from the
aquaculture farm to the consumer’s table.
ID nr. 3796
Menotta° S, Fedrizzi° G, Macrì S, Scandurra S, Saggiorato M
Results of avilamycin residues monitoring plans for the experimental use in Italy
Proceedings of the 9th World rabbit congress : Verona (Italy) June 10-13, 2008 / editors Gerolamo Xiccato,
Angela Trocino and Steven D. Lukefahr. - 1. ed. - Brescia : Fondazione Iniziative Zooprofilattiche e
Zootecniche, 2008. - (Atti delle Giornate di Studio Fondazione Iniziative Zooprofilattiche e Zootecniche ; 72)
p 401
World rabbit congress (9 : Verona (Italy) : June 10-13, 2008)
First author Menotta, Simonetta
Pag. 43 di 300 31/08/2011 The digestive disease is the main cause of mortality in industrial fattening rabbit farms. Recently, avilamycin
has been experimentally used by rabbit producers in Italy as a new option to control digestive syndrome. This
experimental use was exceptionally authorized by the Italian Health Ministry in order to reduce the losses due
to Epizootic Rabbit Enteropathy (ERE) in the rabbit breeding. Although the ERE pathogenesis is not yet
completely known in all its aspects, the presence of Clostridium perfringens has been reported as associated
agent in most of cases. The objective avilamycin experimental use was to evaluate the efficacy of the drug in
feed for the control of digestive signs associated with Clostridium spp. in rabbits at a dose of 5 mg
avilamycin/kg body weight/day, equivalent to 60=120 g11000 kg of feed on the basis of age, body weight and
feed consumption for all animals in the weaning phase. During the experimental use some different residues
monitoring plans were performed by Italian health authorities and Elanco Animal Health with the aim to ensure
public health on treating food-producing animals with an experimental therapy. According to the guidelines of
the Italian Health Ministry an official avilamycin residues monitoring plan was conducted by Elanco during the
last two years and other experimental plans were performed by regional authorities and foodstuff producers.
An HPLCMS/MS method was developed by Elanco, performed and validated by the "Istituto Zooprofilattico
Sperimentale della Lombardia e dell'Emilia Romagna" according to European legislation (EEC/657/2002) and
applied to monitor avilamycin residues in the different plans actuated in Italy. The Avilamycin was analyzed by
hydrolysis to dichloroisoeverninic acid (DIA), the residual marker analyzed. The avilamycin linear range was
from 50 to 250 gg/kg (approximately concentration of DIA from 10 to 50 pg/kg). Within laboratory data
(reproducibility intra-laboratory) were from 11% to 17% for muscle and liver. Repeatability was included
between 10% and 19% for both tissues. The mean recovery was 85% for muscle and 81% for liver. According
to different monitoring plans, more than 250 samples of rabbit treated with avilamycin were collected and their
tissues (muscles, livers or both tissues) were analyzed. The results obtained from these analyses
demonstrate the very low risk due to residues and the high level of safety for avilamycin used in rabbit as
food-producing animal.
ID nr. 3796a
Menotta° S, Fedrizzi° G, Macrì S, Scandurra S, Saggiorato M
Results of avilamycin residues monitoring plans for the experimental use in Italy
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/Q-Menotta.pdf - ultimo
accesso 23-02-2009. - Vol. 2008). - p 1393-1397. - 8 ref bib
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
First author Menotta, Simonetta
The digestive disease is the main cause of mortality in industrial fattening rabbit farms. Recently, avilamycin
has been experimentally used by rabbit producers in Italy as a new option to control digestive syndrome. This
experimental use was exceptionally authorized by the Italian Health Ministry in order to reduce the losses due
to Epizootic Rabbit Enteropathy (ERE) in the rabbit breeding. Although the ERE pathogenesis is not yet
completely known in all its aspects, the presence of Clostridium perfringens has been reported as associated
agent in most of cases. The objective avilamycin experimental use was to evaluate the efficacy of the drug in
feed for the control of digestive signs associated with Clostridium spp. in rabbits at a dose of 5 mg
avilamycin/kg body weight/day, equivalent to 60=120 g11000 kg of feed on the basis of age, body weight and
feed consumption for all animals in the weaning phase. During the experimental use some different residues
monitoring plans were performed by Italian health authorities and Elanco Animal Health with the aim to ensure
public health on treating food-producing animals with an experimental therapy. According to the guidelines of
the Italian Health Ministry an official avilamycin residues monitoring plan was conducted by Elanco during the
last two years and other experimental plans were performed by regional authorities and foodstuff producers.
An HPLCMS/MS method was developed by Elanco, performed and validated by the "Istituto Zooprofilattico
Sperimentale della Lombardia e dell'Emilia Romagna" according to European legislation (EEC/657/2002) and
applied to monitor avilamycin residues in the different plans actuated in Italy. The Avilamycin was analyzed by
hydrolysis to dichloroisoeverninic acid (DIA), the residual marker analyzed. The avilamycin linear range was
from 50 to 250 gg/kg (approximately concentration of DIA from 10 to 50 pg/kg). Within laboratory data
(reproducibility intra-laboratory) were from 11% to 17% for muscle and liver. Repeatability was included
between 10% and 19% for both tissues. The mean recovery was 85% for muscle and 81% for liver. According
to different monitoring plans, more than 250 samples of rabbit treated with avilamycin were collected and their
Pag. 44 di 300 31/08/2011 tissues (muscles, livers or both tissues) were analyzed. The results obtained from these analyses
demonstrate the very low risk due to residues and the high level of safety for avilamycin used in rabbit as
food-producing animal.
ID nr. 3797a
Rota_Nodari S, Lavazza° A, Candotti° P,
Evaluation of rabbit welfare at stunning and slaughtering in a commercial abattoir
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/W-Rota.pdf - ultimo
accesso 23/02/2009. - Vol. 2008). - p 1239-1243. - 9 rif bib
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
A total of 1020 crossbreed rabbits were individually examined to evaluate their welfare at electric stunning and
slaughtering in a commercial abattoir. Stunning (position of electrodes and repetition of current applications)
and sticking (position, length and depth of the cut) procedures were checked. Rabbits were behaviourally
monitored from current application to death. The stunning system was incorrectly applied one hundred and
ten timer (10.8%). Three rabbits failed to be stunned and were still conscious at sticking. Eighteen rabbits
recovered before the onset of death, as evidenced by the corneal reflex, and in a few cases vocalization (n=3)
and head righting (n=1) were observed. Corneal reflex seemed to be the best indicator of recovery at the
abattoir.
ID nr. 3798
Archetti° L, Tittarelli° C, Cerioli° M, Brivio R, Grilli G, Lavazza° A
Serum chemistry and hematology values in commercial rabbits: preliminary data from industrial
farms in Northern Italy
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/W-Archetti.pdf - ultimo
accesso 23-02-2009. - Vol. 2008). - p 1147-1151. - 14 ref bib
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
Project RELO2003596CUNIBENE - Welfare of farmed rabbits: associated sanitary and production traits Lavazza Dr. Antonio - Capofila - REGIONE - 2003 - 149325,00 EUR - 01/10/2003 0.00.00 29/01/2007 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza Dr.
Antonio - I
2 - UNIV MILANO Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione
di Anat - Grilli Dr. Guido - E
First author Archetti, Ivonne Laura
The aim of this preliminary study was to establish reference normal ranges of hematological and biochemical
parameters in rabbits. A total of 8 industrial farms, located in Brescia, Milano and Bergamo Provinces
(Northern Italy), were monitored during a 4-year period (2004-2007). Farms showed some variability as
regards size, management and production parameters. Blood samples, collected from post weaning (30-45
days old) and growing (57-65 days old) rabbits, as well as restocking females before pregnancy (nulliparous),
breeders at first pregnancy (primiparous), lactating does and breeders at culling (multiparous), were delivered
within three hours to the laboratory. The following analyses were performed on serum samples: alanine
aminotransferase (ALT), aspartate aminotransferase (AST), urea, Phosphorus (P), creatine kinase (CK),
lysozyme, lactate dehydrogeìiase (LDH) and creàtinine. Blood samples were tested for: white blood cells
(WBC), neutrophils, lymphocytes, monocytes, eosinophils, basophils, red blood cells (RBC), platelets (PLT),
hemoglobin (Hg), hematocrit (HCT), mean corpuscular hemoglobin (MCH), mean concentration of hemoglobin
in the corpuscular volume (MCHC), mean corpuscular volume (MCV). Results indicate that hematological and
biochemical data are similar to those described in laboratory rabbits. In particular, the age-dependent trend of
values was in agreement with previous observations. However, in does, a lower level of hemoglobin and
lymphocyte numbers and a higher number of neutrophils and monocytes was observed, probably due to
intensive production and exploitation. On the whole, this may indicate chronic stress under seemingly normal
Pag. 45 di 300 31/08/2011 clinical conditions. LDH and CK values particularly differed from those described for laboratory rabbits. High
urea and creatinine values in lactating does could be traced back to concurrent E. cuniculi infection.
ID nr. 3799
Lavazza° A, Cerioli° M, Martella V, Tittarelli° C, Grilli G, Brivio R, Buonavoglia C
Rotavirus in diarrheic rabbits: prevalence and characterization of strains in italian farms
Proceedings of the 9th World rabbit congress : Verona (Italy) June 10-13, 2008 / editors Gerolamo Xiccato,
Angela Trocino and Steven D. Lukefahr. - 1. ed. - Brescia : Fondazione Iniziative Zooprofilattiche e
Zootecniche, 2008. - (Atti delle Giornate di Studio Fondazione Iniziative Zooprofilattiche e Zootecniche ; 72)
p 228
World rabbit congress (9 : Verona (Italy) : June 10-13, 2008)
Project PRF2003307 - Genetic and antigenic diversity of rotaviruses – study of the evolutionary mechanisms
and implications for diagnosis and vaccination - Gentile Dr. Mattia - UO - MINSAN - 2003 256800,00 EUR - 01/03/2004 0.00.00 - 31/10/2006 0.00.00 - 7 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
First author Lavazza, Antonio
The multifactorial rabbit enteropathy has a great importance in rabbit meat production for its economical
impact. Stating the pathogenic role and importance as primary agent of rotavirus, the purposes was to
estimate the prevalence of lapine rotavirus (LRV), identified by negative staining electron microscopy (nsEM),
on samples from rabbits showing either a "generic" enteropathy or lesions_referable to mucoid enteropathy
caecal impaction and then to relate their presence with the symptoms and lesions observed. During the 20022007, 243 samples taken mainly from rabbits showing catarrhal, haemorrhagic or necrotic entero-tiflitis,
mucoid enteropathy and caecal impaction were examined. By nsEM, the presence of viral particles was
observed in 45.3% of them; rotavirus was identified in 16.0%, coronavirus-like virus in 24.7%, parvovirus in
9.0% and enterovirus-like virus in 51% of the positive samples. In addition, adenovirus, calicivirus and
reovirus were sporadically found and, in 29 cases, 2 or 3 different viruses were contemporarily observed in
association in the same sample. Using the criteria for the classification of rotavirus strains based on the VP4
(P type) and VP7 (G type) genotyping, almost all the strains were characterized as P[22] G3 confirming the
presence of the newly-recognized rotavirus P[22] VP4 allele in Italian rabbits. The availability of the results of
microbiological and parasitological analysis allowed to correlate nsEM observations with the contemporary
presence of others pathogenic agents such as rotavirus, enteropathogenic Escherichia coli (EPEC) and
Staphyloccoccus aureus in suckling rabbits; EPEC, Cl. spiroforme and flagellate protozoa in the others. The
pathogenic role and importance of rotavirus as primary aetiological agent rabbit enteritis are discussed.
ID nr. 3799a
Lavazza° A, Cerioli° M, Martella V, Tittarelli° C, Grilli G, Brivio R, Buonavoglia C
Rotavirus in diarrheic rabbits: prevalence and characterization of strains in italian farms
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/P-Lavazza.pdf - ultimo
accesso 23-02-2009. - Vol. 2008). - p 993-997. - 17 ref bib
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
Project PRF2003307 - Genetic and antigenic diversity of rotaviruses – study of the evolutionary mechanisms
and implications for diagnosis and vaccination - Gentile Dr. Mattia - UO - MINSAN - 2003 256800,00 EUR - 01/03/2004 0.00.00 - 31/10/2006 0.00.00 - 7 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
First author Lavazza, Antonio
The multifactorial rabbit enteropathy has a great importance in rabbit meat production for its economical
impact. Stating the pathogenic role and importance as primary agent of rotavirus, the purposes was to
estimate the prevalence of lapine rotavirus (LRV), identified by negative staining electron microscopy (nsEM),
Pag. 46 di 300 31/08/2011 on samples from rabbits showing either a "generic" enteropathy or lesions_referable to mucoid
enteropathycaecal impaction and then to relate their presence with the symptoms and lesions observed.
During the 2002-2007, 243 samples taken mainly from rabbits showing catarrhal, haemorrhagic or necrotic
entero-tiflitis, mucoid enteropathy and caecal impaction were examined. By nsEM, the presence of viral
particles was observed in 45.3% of them; rotavirus was identified in 16.0%, coronavirus-like virus in 24.7%,
parvovirus in 9.0% and enteffivirus-like virus in 51% of the positive samples. In addition, adenovirus,
calicivirus and reovirus were sporadically found and, in 29 cases, 2 or 3 different viruses were contemporarily
observed in association in the same sample. Using the criteria for the classification of rotavirus strains based
on the VP4 (P type) and VP7 (G type) genotyping, almost all the strains were characterized as P[22] G3
confirming the presence of the newly-recognized rotavirus P[22] VP4 allele in Italian rabbits. The availability of
the results of microbiological and parasitological analysis allowed to correlate nsEM observations with the
contemporary presence of others pathogenic agents such as rotavirus, enteropathogenic Escherichia coli
(EPEC) and Staphyloccoccus aureus in suckling rabbits; EPEC, Cl. spiroforme and flagellate protozoa in the
others. The pathogenic role and importance of rotavirus as primary aetiological agent rabbit enteritis are
discussed.
ID nr. 3800
Cerioli° M, Brivio R, Grilli G, Tittarelli° C, Marasciulo V, Lavazza° A
Search for key health and welfare indicators for meat rabbit production and definition of a score
method of evaluation
Proceedings of the 9th World rabbit congress : Verona (Italy) June 10-13, 2008 / editors, Gerolamo Xiccato,
Angela Trocino and Steven D. Lukefahr. - 1 ed. - Brescia : Fondazione Iniziative Zooprofilattiche e
Zootecniche, 2008. - (Atti delle Giornate di Studio Fondazione Iniziative Zooprofilattiche e Zootecniche ; 72)
p 273
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
Project RELO2003596CUNIBENE - Welfare of farmed rabbits: associated sanitary and production traits Lavazza Dr. Antonio - Capofila - REGIONE - 2003 - 149325,00 EUR - 01/10/2003 0.00.00 29/01/2007 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza Dr.
Antonio - I
2 - UNIV MILANO Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione
di Anat - Grilli Dr. Guido - E
First author Cerioli, Monica
The aim of this study is to define parameters related to health and welfare of animals even in industrial farms
with intensive husbandry. Therefore, we tried to increase the knowledge on rabbit welfare by the use of
correct tools for monitoring the different aspects of rabbit industrial farming, in order to improve the efficieney
of farms and the productive performances of animals. Our study is based firstly on the charaeterization of
health, management, environmental and physiological parameters and, secondly, on the definition of
protocols to assess welfare of rabbits. All the entered values are then utilized for a score system to establish
health and welfare conditions in industrial farms.
ID nr. 3800a
Cerioli° M, Brivio R, Grilli G, Tittarelli° C, Marasciulo V, Lavazza° A
Search for key health and welfare indicators for meat rabbit production and definition of a score
method of evaluation
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/P-Cerioli.pdf - ultimo
accesso 20-02-2009. - Vol. 2008). - p 915-919. - 6 bib ref
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
Project RELO2003596CUNIBENE - Welfare of farmed rabbits: associated sanitary and production traits Lavazza Dr. Antonio - Capofila - REGIONE - 2003 - 149325,00 EUR - 01/10/2003 0.00.00 29/01/2007 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza Dr.
Pag. 47 di 300 31/08/2011 Antonio - I
2 - UNIV MILANO Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione
di Anat - Grilli Dr. Guido - E
First author Cerioli, Monica
The aim of this study is to define parameters related to health and welfare of animals even in industrial farms
with intensive husbandry. Therefore, we tried to increase the knowledge on rabbit welfare by the use of
correct tools for monitoring the different aspects of rabbit industrial farming, in order to improve the efficieney
of farms and the productive performances of animals. Our study is based firstly on the charaeterization of
health, management, environmental and physiological parameters and, secondly, on the definition of
protocols to assess welfare of rabbits. All the entered values are then utilized for a score system to establish
health and welfare conditions in industrial farms.
ID nr. 3801
Lavazza° A, Capucci° L
Viral infection of rabbits
Proceedings of the 9th World rabbit congress : Verona (Italy) June 10-13, 2008 / editors Gerolamo Xiccato,
Angela Trocino and Steven D. Lukefahr. - 1. ed. - Brescia : Fondazione Iniziative Zooprofilattiche e
Zootecniche, 2008. - (Atti delle Giornate di Studio Fondazione Iniziative Zooprofilattiche e Zootecniche ; 72)
p 247-267. - 87 bib ref
World rabbit congress (9 : Verona (Italy) : June 10-13, 2008)
First author Lavazza, Antonio
The three most important viruses of rabbits include: Myxoma virus (MV), the poxvirus that causes
Myxomatosis, the calicivirus (genus Lagovirus) of Rabbit Haemorrhagic Disease (RHDV), and Lapine
Rotavirus (LRV), which is an enteric agent. There are some other viral agents in rabbits (parvovirus,
coronavirus, adenovirus, calicivirus (genus Vesivirus), enterovirus-like, reovirus, herpesvirus and coronavirus)
but both their occurrence and their pathological value are negligible. Myxomatosis was firstly introduced in
Europe more than 50 years ago and still represents a current and real problem. Recent studies have been
focused to determine the role and function of the over 100 genes of MV encoding structural and essential
proteins; in particular the immunomodulatory MV (im-MV) proteins (virokines and viroreceptors, immune
modulators and anti-apoptotic factor), involved in contrasting the host immune system response towards the
MV infection. One of the main tools to control myxomatosis in endemic areas is use of the live attenuated
vaccines that are able to induce traceable humoral immunity for a variable time even if the protection of
rabbits from the infection is not fully guarantee. Therefore, thanks allo to the knowledge on the im-MV
proteins, a new family of biotechnology deleted vaccines will soon be produced and made available in a few
years. These should be more safe and able to induce a wider immunity and permit to apply the DIVA strategy
i.e. to use serology to ascertain if the anti-MV antibodies detected in a rabbits originate by an infection or a
vaccination. RHDV is a non-cultivable calicivirus that infects rabbits and causes an acute fatal hepatitis, firstly
described in China in 1984. The first consistent antigenic variant called RHDVa, was identified in Italy and
Germany in 1997. Nowadays it is present in most parts of Italy and its field prevalence has reached value over
50%. In Europe it has been reported between 1997 and 2004 in Germany, France, Malta and Hungary.
Outside Europe, it was reported in Reunion Islands, USA and South America and, taking account of the
RHDV genetic sequences deposited at the NCBI databank, its presence in China is also evident from 1985.
More recently some other RHDV isolates presenting peculiar characteristics were identified. Based on their
reactivity with MAbs these strains could be considered as further and separate steps of variation of the
RHDVa, possibly classified as sub-variants. The detection of seropositivity for RHDV in the sera of healthy
farmed and laboratory rabbits taken between 1975 and 1985, which is approximately ten years before the
occurrence of the disease in Europe, has suggested the hypothesis of the existence of one or more "nonpathogenic" viral strains antigenically related to pathogenic RHDV. Rabbit Calicivirus (RCV), the first of these
non-pathogenic RHDV-like viruses identified in healthy rabbits, was detected in Italy in 1996. It is avirulent,
replicates in the intestine at a low titre and presents a 92% genomic identity with RHDV. The diffusion of RCV
in different areas of Italy has been evaluated in five consecutive serological surveys during the period 19992008, throughout the determination at slaughtering of anti-RHD antibodies in non-vaccinated meat rabbits
from RHDV-free commercial rabbitries. The results clearly show that antibodies reactive with RHDV are
Pag. 48 di 300 31/08/2011 present in several rabbit populations: almost 30% of controlled farms and over 80% of animals. The definitive
proofs that an active infection had occurred came from the detection of IgA and IgM as well as the
identification of viral strains by using PCR on faeces. In addition, the existence of other non-pathogenic
caliciviruses in wild rabbits was suggested by the serological surveys of rabbit populations in European
countries (UK and France), Australia and New Zealand. Either the identification of RNA particles related to
RHDV in rabbit sera collected since 1955 in Britain and the very recent isolation and identification of one of
these viruses in Australia finally confirmed such hypothesis. Enteric diseases have an important role in the
rabbit industry since they produce severe economic losses due to mortality, growth depression and worsening
of conversion index. Among the different pathogens that could be found in rabbits suffering from enteropathy,
viruses seem to have an important but not definitive role. Viruses and among others Lapine Rotavirus (LRV)
particularly, should not be able to induce primary episodes of high gravity but, acting as mild pathogens, they
have the capacity of became endemic. The role and importance of viruses as primary aetiological agent of
rabbit enteritis are here discussed, by both reviewing the available literature and presenting the results of
studies of prevalence of the viruses identified in rabbits with enteritis. That is to recognise the main features
and pathogenic abilities of different viral agents and to try to attribute them an etiological role in enteric
syndromes, relating their presence with pathologic lesions.
ID nr. 3801a
Lavazza° A, Capucci° L
Viral infection of rabbits
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/P0-LavazzaCapucci.pdf
- ultimo accesso 20-02-2009. - Vol. 2008). - p 879-893. - 87 bib ref
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
First author Lavazza, Antonio
The three most important viruses of rabbits include: Myxoma virus (MV), the poxvirus that causes
Myxomatosis, the calicivirus (genus Lagovirus) of Rabbit Haemorrhagic Disease (RHDV), and Lapine
Rotavirus (LRV), which is an enteric agent. There are some other viral agents in rabbits (parvovirus,
coronavirus, adenovirus, calicivirus (genus Vesivirus), enterovirus-like, reovirus, herpesvirus and coronavirus)
but both their occurrence and their pathological value are negligible. Myxomatosis was firstly introduced in
Europe more than 50 years ago and still represents a current and real problem. Recent studies have been
focused to determine the role and function of the over 100 genes of MV encoding structural and essential
proteins; in particular the immunomodulatory MV (im-MV) proteins (virokines and viroreceptors, immune
modulators and anti-apoptotic factor), involved in contrasting the host immune system response towards the
MV infection. One of the main tools to control myxomatosis in endemic areas is use of the live attenuated
vaccines that are able to induce traceable humoral immunity for a variable time even if the protection of
rabbits from the infection is not fully guarantee. Therefore, thanks allo to the knowledge on the im-MV
proteins, a new family of biotechnology deleted vaccines will soon be produced and made available in a few
years. These should be more safe and able to induce a wider immunity and permit to apply the DIVA strategy
i.e. to use serology to ascertain if the anti-MV antibodies detected in a rabbits originate by an infection or a
vaccination. RHDV is a non-cultivable calicivirus that infects rabbits and causes an acute fatal hepatitis, firstly
described in China in 1984. The first consistent antigenic variant called RHDVa, was identified in Italy and
Germany in 1997. Nowadays it is present in most parts of Italy and its field prevalence has reached value over
50%. In Europe it has been reported between 1997 and 2004 in Germany, France, Malta and Hungary.
Outside Europe, it was reported in Reunion Islands, USA and South America and, taking account of the
RHDV genetic sequences deposited at the NCBI databank, its presence in China is also evident from 1985.
More recently some other RHDV isolates presenting peculiar characteristics were identified. Based on their
reactivity with MAbs these strains could be considered as further and separate steps of variation of the
RHDVa, possibly classified as sub-variants. The detection of seropositivity for RHDV in the sera of healthy
farmed and laboratory rabbits taken between 1975 and 1985, which is approximately ten years before the
occurrence of the disease in Europe, has suggested the hypothesis of the existence of one or more "nonpathogenic" viral strains antigenically related to pathogenic RHDV. Rabbit Calicivirus (RCV), the first of these
non-pathogenic RHDV-like viruses identified in healthy rabbits, was detected in Italy in 1996. It is avirulent,
replicates in the intestine at a low titre and presents a 92% genomic identity with RHDV. The diffusion of RCV
in different areas of Italy has been evaluated in five consecutive serological surveys during the period 19992008, throughout the determination at slaughtering of anti-RHD antibodies in non-vaccinated meat rabbits
Pag. 49 di 300 31/08/2011 from RHDV-free commercial rabbitries. The results clearly show that antibodies reactive with RHDV are
present in several rabbit populations: almost 30% of controlled farms and over 80% of animals. The definitive
proofs that an active infection had occurred came from the detection of IgA and IgM as well as the
identification of viral strains by using PCR on faeces. In addition, the existence of other non-pathogenic
caliciviruses in wild rabbits was suggested by the serological surveys of rabbit populations in European
countries (UK and France), Australia and New Zealand. Either the identification of RNA particles related to
RHDV in rabbit sera collected since 1955 in Britain and the very recent isolation and identification of one of
these viruses in Australia finally confirmed such hypothesis. Enteric diseases have an important role in the
rabbit industry since they produce severe economic losses due to mortality, growth depression and worsening
of conversion index. Among the different pathogens that could be found in rabbits suffering from enteropathy,
viruses seem to have an important but not definitive role. Viruses and among others Lapine Rotavirus (LRV)
particularly, should not be able to induce primary episodes of high gravity but, acting as mild pathogens, they
have the capacity of became endemic. The role and importance of viruses as primary aetiological agent of
rabbit enteritis are here discussed, by both reviewing the available literature and presenting the results of
studies of prevalence of the viruses identified in rabbits with enteritis. That is to recognise the main features
and pathogenic abilities of different viral agents and to try to attribute them an etiological role in enteric
syndromes, relating their presence with pathologic lesions.
ID nr. 3802
Angelini P, Macini P, Finarelli AC, Po C, Venturelli C, Bellini R, Dottori° M
Chikungunya epidemic outbreak in Emilia-Romagna (Italy) during summer 2007
Parassitologia. - Vol. 50 no 1-2 ( 2008). - p 97-98. - 6 bib ref
Congresso Nazionale della Societa' Italiana di Parassitologia (25. : Pisa : 18-21 giugno 2008)
During summer 2007, an outbreak due to the loca) transmission of CHIKV by Aedes albopictus mosquitoes
occurred moreover in Italy, Emilia-Romagna Region, in the areas of Ravenna, Forlì-Cesena, Rimini and
Bologna cities.The originai outbreak developed in Castiglione di Cervia and Castiglione di Ravenna, two small
villages divided by a river. The first case was recorded on August 9th the epidemic out-break then spread out,
thus giving rise to smaller secondary outbreaks and further sporadic cases in the same area, for a total of 337
suspected cases, 217 of which confirmed by blood analysis. CHIKV has been isolated and characterized on
both blood and mosquito samples.
ID nr. 3804
Bertasi° B, Losio° MN, Finazzi° G, Tilola° M, Maccabiani° G, Boni° P
Ribotyping applications in the field of epidemiological survey: identification and characterisation of
Listeria monocytogenes strains
Ind Aliment (Pinerolo). - Vol. 47 no 481 ( 2008). - p 626-630. - 7 bib ref
Project RELO200589METADIS - Bio-technology for food safety: evaluation of products and process
changes toward high-safety products. - Zani Sig. Diego - UO - REGIONE - 2005 - 1138790,05
EUR - 01/01/2005 0.00.00 - 27/06/2007 0.00.00 - 1 - I3 - Reparto Microbiologia e Parassitologia
degli Alimenti e Sorveglianza Epidemiologica - Boni Dr. Paolo - I
First author Bertasi, Barbara
Listeria monocytogenes is on ubiquitous foodborne pathogen; some strains of this microorganism can cause
listeriosis, it can become a serious disease in immunocompromised people. Genotypic characterisation
methodis are use-ful to study pathogenicity/strains associotions, because traditional techniques are influenced
by environmental factors insteod. Automotic ribotyping can be used to identify and characterise strains
isolated from food, environmental samples and biological samples, to identify eventual correlations and to
perform a more specific risk analysis.
Pag. 50 di 300 31/08/2011 ID nr. 3806
Losi° CG, Ferrari° S, Sossi° E, Villa° R, Ferrari° M
An alternative method to isoenzyme profile for cell line identification and interspecies crosscontaminations: cytochrome b PCR-RLFP analysis
In Vitro Cell Dev Biol Anim. - Vol. 2008). - p . -27 bib ref
Impact factor: 0,660
Project PRC2007011 - Development and improvement of cryoconservation procedures for cells used in
regenerative medicine - Ferrari Dr.ssa Maura - Capofila - MINSAN - 2007 - 50000,00 EUR 01/09/2008 0.00.00 - 31/08/2010 0.00.00 - 1 - L1 - Reparto Substrati Cellulari - Ferrari Dr.ssa
Maura - I
2 - Istituto di Tec.gie Biom.che CNR - Biunno Dr.ssa Ida - E
First author Losi, Claretta_Gioia
One of the major risks in cell culture laboratories is the misidentification and cross-contamination of cell lines.
Several methods have been used to authenticate cell lines, including isoenzyme profiling, the test suggested
by European Farmacopeia, which is performed at the Tissue Culture Centre in Brescia. However, this method
displays several disadvantages, such as high variability and low reproducibility, and it is time consuming and
requires high cell concentrations to be perfonned. Therefore, an alternative method has been developed to
continn the specie of origin of 27 different animal cell cultures. A polymerase chain reaction (PCR)--restriction
fragment length polymorphism (RFLP) assay was optimized, based on the use of a pair of primers that anneal
to a portion of the cytochrome b gene in ali the species. The ampilification product was digested with a panel
of six restriction enzymes, and the pattem derived was resolved on 3% high-resolution agarose gel. For 23
species, this protocol produced a unique restriction pattem, and the origin of these animal cells resulted to be
confirmed by this analysis. Furthennore, results indicate that cytochrome b PCRRFLP was able to amplify
target sequences using very low amounts of deoxyribonucleic acid (DNA). Its sensitivity in detecting
interspecies, cross-contamination was compamble to that of isoenzyme analysis (contaminating DNA should
represent at least 10% of the total DNA). For 4 of the 27 species (sheep, dog, Guinea pig, and Rhesus
monkey) the observed pattem, even if highly reproducible, showed additional bands; for these species,
specific PCR was also perfomed.
ID nr. 3807
Sesso L, Losi° GC, Renzi S, Torre ML, Galdi A, Russo V, Vigo D, Ferrari° M
Preliminary study of isolation, amplification and characterization of equine adipose derived
mesenchymal stem cells
Atti Soc Ital Sci Vet. - Vol. 62 ( 2008). - cdrom p 95-96. - 4 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (62 : S. Benedetto del
Tronto (AN) : 2008)
First author Ferrari, Maura
Mesenchymal stem cells (MSCs) have been isolated from a variety of tissues
and tested for differentiation into different cell lineages. MSCs have been used experimentally and in limited
numbers of clinical cases in the equine orthopaedic field. The aim of this study is to develop a protocol for the
isolation, amplification and finally the characterization of equine
adipose derived mesenchymal stem cells (ADSC). Fibroblastic-like cell coltures were obtained by the adipose
tissue enzymatic digestion. These cells were able to grow, in differentiation medium, toward the adipogenic
lineage. This study is the first step for a potential use of cell therapy in equine orthopaedic field, in particular
for tendon re-construction. Further investigations will be conducted to better characterize equine ADSC.
ID nr. 3808
Losio° MN, Lombardi° G, Luppi° A, Pavoni° E, Faita° R, Lavazza° A, Cordioli° P, Boni° P
Pag. 51 di 300 31/08/2011 Detection and molecular identification of Hepatitis E virus (HEV) in swine faces
20th IPVS Internationl Pig Veterinary Society Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - V. 2. - cdrom p 11. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, South Africa : 22-26 June 2008)
First author Losio, Marina Nadia
not found.
ID nr. 3809
Lombardi° G, Pavoni° E, Faita° R, Losio° MN, Luppi° A, Gelmetti° D, Cordioli° P, Boni° P
Experimental infection of SPF pigs with hepatitis e virus (HEV)
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 12. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Project PRC2004008 - Preliminary assessment of the presence of hepatitis E infection in swine of
Lombardy, set-up of an ELISA serological method and PCR - Lombardi Dr. Guerino - Solo MINSAN - 2004 - 50000,00 EUR - 01/07/2005 0.00.00 - 30/06/2007 0.00.00 - 1 - G3 - Reparto
benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Lombardi Dr. Guerino - I
First author Lombardi, Guerino
not found.
ID nr. 3810
Moreno_Martin° A, Barbieri° I, Sozzi° E, Luppi° A, Canelli° E, Foni° E, Alborali° L, Cordioli°
P
Genetic characterization of swine influenza H3N2 subtype in Itlay
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 25. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Project RELO2005910SUINFL - Diagnostic and control strategies for respiratory diseases of swine, with
emphasis on influenza virus infection and the epidemiological role of pigs with respect to avian
influenza virus strains. - Alborali Dr. Loris - Capofila - REGIONE - 2005 - 65000,00 EUR 01/05/2005 0.00.00 - 01/03/2009 0.00.00 - 1 - M1 - Brescia - Alborali Dr. Loris - I
2 - ARAL - Crema - Battaglia Dr. Massimo - E
First author Moreno_Martin, Ana
not found.
ID nr. 3811
Moreno_Martin° A, Barbieri° I, Sozzi° E, Luppi° A, Lelli° D, Lombardi° G, Zanoni° MG,
Cordioli° P
Novel swine influenza virus subtype H3N1 in Italy
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 26. - 6 bib ref
Pag. 52 di 300 31/08/2011 International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Project RELO2005910SUINFL - Diagnostic and control strategies for respiratory diseases of swine, with
emphasis on influenza virus infection and the epidemiological role of pigs with respect to avian
influenza virus strains. - Alborali Dr. Loris - Capofila - REGIONE - 2005 - 65000,00 EUR 01/05/2005 0.00.00 - 01/03/2009 0.00.00 - 1 - M1 - Brescia - Alborali Dr. Loris - I
2 - ARAL - Crema - Battaglia Dr. Massimo - E
First author Moreno_Martin, Ana
not found.
ID nr. 3812
Kiriakis CS, Brown IH, Foni° E, Kuntz_Simon G, Moldonado J, Van_Reeth K
Virological surveillance for swine influenza in Europe
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 30. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Project ESNIP2 - "European surveillance network for influenza in pigs 2 (ESNIP 2). - Van Reet dr.ssa
Kristien - UO - CE - 2005 - 300000,00 EUR - 01/01/2006 0.00.00 - 31/03/2009 0.00.00 - 4 - N5
- Parma - Foni Dr.ssa Emanuela - I
not found.
ID nr. 3813
Gavaudan S, Barocci S, Briscolini S, Mancini P, Morandi F, Paniccià M, Ferrari° M, Villa°
R, Petrini S
Characterization of Porcine Circovirus Type 2 (PCT2) in wild boars in central Italy
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 63. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3814
Candotti° P, Dotti° S, Guana S, Rota_Nodari S, Amadori° M, Villa° R, Petrini S, Lombardi°
G, Ferrari° M
Susceptibility of pure bred large white and landrace pigs to experimental infection with porcine
respiratory and reproductive virus (PRRSV)
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 122. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Project PRC2002013 - Susceptibility of different pig breeds to PRRS - Candotti Dr. Paolo - Capofila MINSAN - 2002 - 139810,29 EUR - 15/02/2005 0.00.00 - 14/02/2009 0.00.00 - 1 - G3 - Reparto
benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Candotti Dr. Paolo - I
2 - CERSA - Giuffra Dr.ssa Elisabetta - E
3 - UNI MI - Domeneghini Dr.ssa Cinzia - E
4 - IBBA CNR - Boettcher Dr. Paul - E
Pag. 53 di 300 31/08/2011 First author Candotti, Paolo
In this study, twenty pigs, thirty day old of two different pure bred lines: fourteen Large White and fourteen
Landrace, were experimentally infected with PRRS virus. The animals were investigated for thirty days by
clinical, virological and à immunological tests, to compare the infection response differences between two pig
lines. The collected data seem to evidence a more severe clinical sign in Large White aniimals than in
Landrace and tris finding could be in relationship with different values of IL-10, IFI –(ypsilon) SC and IgM, that
were detected in the two pure bred lines.
ID nr. 3815
Candotti° P, Dotti° S, Amadori° M, Villa° R, Lombardi° G, Rota_Nodari S, Ferrari° M
Experimental infection of pure bred landrace pigs with porcine respiratory and reproductive virus
(PRRSV) : clinical and laboratory findings
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 123. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Project PRC2002013 - Susceptibility of different pig breeds to PRRS - Candotti Dr. Paolo - Capofila MINSAN - 2002 - 139810,29 EUR - 15/02/2005 0.00.00 - 14/02/2009 0.00.00 - 1 - G3 - Reparto
benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Candotti Dr. Paolo - I
2 - CERSA - Giuffra Dr.ssa Elisabetta - E
3 - UNI MI - Domeneghini Dr.ssa Cinzia - E
4 - IBBA CNR - Boettcher Dr. Paul - E
First author Candotti, Paolo
not found.
ID nr. 3816
Sandri G, Pesente P, Bittesnik S, Giovanardi D, Ferrari° M
Real time RT-PCR : a very sensitive diagnostic tool to detect low positives in a PRRS eradication
programme
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 141. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3817
Lavazza° A, Tittarelli° C, Cerioli° M, Luppi° A, Alborali° G, Cordioli° P
Electronmicroscopic detection of viral agents in diarrhoeic pigs during the period 2002-2007 in
Northern Italy
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 180. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
First author Lavazza, Antonio
Enteric viral diseases are important economic problems affecting pig production. The aim of this study is to
Pag. 54 di 300 31/08/2011 report the data of identification by IEM of viral agents in diarrheic pigs during the period 2002-2007 in Northern
Italy, by using both hyperimmune and convalescent sera. This study shows that IEM could be very useful
since they permit to detect not cultivable virions and multiple viral infections. Indeed it gives a good diagnostic
indication in a very short time (few hours) and it valuable indication for further investigation.
ID nr. 3818
Morandi F, Ostanello F, Fusaro L, Bacci B, Nigrelli° A, Alborali° L, Dottori° M, Vezzoli° F,
Barigazzi° G, Fiorentini° L, Sala V, Leotti G, Joisel F, Sarli G
Immunohistochemical investigation on proliferative and necrotizing pneumonia (PNP) in Italy:
preliminary results
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 181. - 9 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3819
Martinez E, Maldonado J, Barigazzi° G, Riera P
Ambiguous serotype identification of biotype 2 Actinobacillus pleuropneumonie
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 232. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3820
Luppi° A, Bonilauri° P, Di_Lecce R, Paoletti F, Bosetti M, Cordioli° P
Clinical and pathological investigation in an outbreak of PMWS and diagnostic methods comparison
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 49. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
First author Luppi, Andrea
The aim of this study is to establish the grading of lymphoid lesions, after a cytological, histological and real
time PCR examination in order to evaluate the reliability of the cytology in PMWS diagnosis. In conclusion, in
our experience, cytological examination may be considered an important complementary diagnostic tool for
the diagnosis of PMWS.
ID nr. 3821
Magistrali C, Catanossi M, Marenzoni ML, Sensi M, Crotti S, Valente C, Merialdi° G,
Maresca C
Agreement between different diagnostic tests for Brachyspira pilosicoli and Brachyspira
hyodysenteriae infections in swine
Pag. 55 di 300 31/08/2011 20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 248. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3822
Martelli P, Merialdi° G, Bonilauri° P, Bertacchini F, Greatti S, Van_Groenland GJ
Elimination of PRRSV, Micoplasma hyopneumoniae, Actinobacillus pleuropneumoniae by
segregated medicated early weaning
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 365. - 1 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3823
Merialdi° G, Bonilauri° P, Dottori° M, Nigrelli° A, Martelli P
Monitoring respiratory disease at slaughterhouse using lung and pleural lesions score and serology
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 380. - 1 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
First author Merialdi, Giuseppe
not found.
ID nr. 3824
Corradi A, Jottini S, Lavazza° A, Cantoni AM
Skin papillomatosis in piglets
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 547. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Papillomatosis is a rare benign tumor of papovaviral origin, self-limitating and transmissible, that affects
genitalia and/or skin in pig and that is rarely described. The occurrence of papillomatosis in two piglets is
reported and histological and immuno-histochemical lesions are described.
ID nr. 3825
Nigrelli° A, Rosignoli° C, Faccini° S
Gross pathology and pathogens in weaned piglets in italian swine farms (years 2004-2005)
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 560. - 9 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
Pag. 56 di 300 31/08/2011 Project PRC2005015 - Preliminary study for the development of a model for the surveillance of Salmonella
enterica infection in fattened pigs and for the assessment of prevalence decrease over time - Nigrelli
Dr. Arrigo - Solo - MINSAN - 2005 - 139780,00 EUR - 01/08/2006 0.00.00 - 30/09/2008 0.00.00 1 - M6 - Mantova - Nigrelli Dr. Arrigo - I
2 - M4 - Cremona - Boldini Dr. Massimo - I
3 - M5 - Lodi - Luini Dr. Mario - I
4 - M8 - Pavia - Fabbi Dr. Massimo - I
5 - M1 - Brescia - Alborali Dr. Loris - I
First author Nigrelli, Arrigo Daniele
not found.
ID nr. 3826
Di_Bartolo I, Martelli F, Toma S, Inglese N, Caprioli A, Ruggeri FM, Lelli° D, Bonci° M,
Ostanello F
Molecular characterisation and phylogenesis of swine hepatitis E virus (HEV) strains identified in
Italy
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 2. - 5 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
ID nr. 3827
Martelli F, Toma S, Di_Bartolo I, Inglese N, Caprioli A, Ruggeri FM, Lelli° D, Bonci° M,
Ostanello F
Epidemiology of hepatitis E virus (HEV) strains identified in Italian pigs affected by different
pathological conditions
20th IPVS Internationl Pig Veterinary Science Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - cdrom p 5. - 7 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3828
Sarli G, Ostanello F, Morandi F, Fusaro L, Bacci B, Nigrelli° A, Alborali° L, Dottori° M,
Vezzoli° F, Barigazzi° G, Fiorentini° L, Sala V, Leotti G
Diagnosis of PMWS in Italy
Large Anim Rev. - Vol. 14 no 3 ( 2008). - p 119-126. - 21 bib ref
First author Nigrelli, Arrigo Daniele
From the beginning of October 2006 to January 31' 2008, in the context of a work sponsored by Merial Italia, a
diagnostic pathway has been developed for the study of post-weaning multisystemic wasting syndrome
(PMWS) of pigs. Superficial inguinal and bronchial lymph nodes, ileum, tonsil and lung from 3 to 5 subjects
(229 pigs belonging to 64 farms from North and Centre Italy) were collected. The samples were valued by
histological and immunohistochemical (IHC) assays for porcine circovirus-2 (PCV2). The diagnosis confirmed
PMWS in those cases with inclusion bodies or where the presence of a positive IHC stain was contextual to
the lesions indicative of PMWS in the lymphoid tissue (depletion and/or granulomas) and/or lung (interstitial
pneumonia). In the other cases the samples were considered PCV2 infected when PCV2-positive cells were
present without PMWS lesions; negative in the absence of PCV2 infection and PMWS lesions. PMWS was
Pag. 57 di 300 31/08/2011 diagnosed on 48 farms; 21 where the total number of examined subjects had a conclusive diagnosis of
PMWS, whereas the diagnosis could be considered conclusive for PMWS only for some animals on the
remaining 27. The protocol proved more reliable when appled to a group of animals than to a single pig.
ID nr. 3829
Bacci B, Morandi F, Ostanello F, Alborali° L, Sarli G
Diagnosis of proliferative enteropathy by Lawsonia intracellularis
Large Anim Rev. - Vol. 14 no 3 ( 2008). - p 115-118. - 21 bib ref
Proliferative enteropathy (PE) is a common intestinal disease of pigs caused by Lawsonia intracellularis, an
obligate intracellular bacterium. The disease includes a group of acute and chronic conditions with a common
pathological change characterized by a thickening of the mucosa in the small intestine and colon.
Histologically the affected tissues show marked proliferation of immature epithelial cells of intestin al crypts.
The proliferating cells invariably contain numerous intracytoplasmic bacteria. Diagnostic tools have improved
overall detection and early onset of PE in pigs, include various serological and molecular-based assays.
Histological tests and immunohistochemistry continue to be the gold standard for the post mortem diagnosis
of the disease.
ID nr. 3830
Bellini° S, Bugnetti° M, Grazioli° S, Brocchi° E
Swine Vesicular Disease (SVD): disease situation, epidemiology, diagnosis, prevention and control
measures
Workshop Epizone "Research in swine viral disease "Building Bridges" : 7-9 July 2008, Shanghai / [s.n. : s.
n., 2008]. - 53 p.
Workshop Epizone : Shanghai : 7-9 July 2008)
Project Epizone - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Bellini, Silvia
SVD was first observed in Italy in 1966, when it was clinically recognised as foot-and-mouth disease (FMD).
Despite clinical signs, bio - chemical properties of the virus showed that it differed from FMD, in fact, SVDV
was classified as an Enterovirus within the Picornaviridae family. SVDV causes a vesicular diseasè in pigs but
does not affect ruminants. In Europe, SVD affected Netherlands, Belgium, Spain, Portugal and Italy in the
early "90"s. Since then only Italy and Portugal have reported outbreaks. The last reported case of SVD from
the Far East was in Taiwan in 1999. SVDV is extremely resistant in the environment and to normal
disinfectants, its stability in the environment is a feature of major importance in the epidemiology and control
of the disease. Direct contact of susceptible animals with infected pigs or their excretions is usually necessary
for disease transmission. SVD has a limited tendency to diffuse, even within infected premises, it is not
transmitted by the airborne route, spread from one pen to an other may not happen in absence of movement
of infected pigs or contaminated material. Swill feeding has been considered a risk for the spread of SVD.
SVD diagnostic procedures are lay down at Community level in Commission Decision 2000/428/EC.
Currently, very sensitive and specific tests are available for SVD diagnosis and for the differentiation with
FMD. Control measures are established in EU by Council Directive 92/119/EEC and are activated on the
basis of clinical suspicion, even though experience from the field indicates that clinical severity of SVD
appears to have decreased and sub clinical infection is now more common than clinical form.
ID nr. 3831
Pag. 58 di 300 31/08/2011 Varisco° G, Bertocchi° L, Contessi N, Tranquillo° M, Bolzoni° G
Bulk milk tank Staphylococcus aureus UCF count as predictive datum for bovine herd's infection
level
Hung Vet J. - Vol. 130 Suppl 2 ( 2008). - p 62 (Poster 587)
Jubilee World Buiatrics Congress (25. : Budapest (Hungary) : July 6-11, 2008)
First author Varisco, Giorgio
Aim of this work is to verify the predictivity of bulk milk tank (BMT) analysis as simple tool for epidemiological
surveillance system on herd's Staphylococcus aureus infection level. The comparison between BMT analysis
results and single cow milk (SCM) analysis results was investigated to verify the relationship between BMT
Staph. aureus UFC value and dairy herds infection rate. During 2005, 47.208 cow milk samples were
analysed (7.166 positive, 38.530 negative and 1.512 to much polluted) of 31 dairy herds (approximately 4.000
cows were checked). Staph. aureus count in BMT (UFC) was found to decrease during time, due to dairy
Staph. aureus infection rate red at cow level. Statistical analysis shows that within herds the prevalence is
1.28 grater (exp 0.25) every one UFC log unit (p<0.001). UFC variable did not show norma) distribution
therefore it was been transformed in log. the mode) allow to calculate odds log for an infected herd.
a) log (odds) = 2,829 + 0,25 * (ufc) To obtain prevalence value the following formula is to be used:
b) Prevalence= Odds11+odds where odds value is obtained using natural value formula a) result Ex: BMT
results is 3UFC (Staph. aureus) Log(odds)= -2.829+025*(3)= -2.079natural vale: exp.079)= 0.1250
prevalence= 0.12501(1+0.1250)= 0.107 (10.7%) In conclusion: results obteined confirm the significative
statistical relationship between UFC, in log, and cows' infection rate; BMT analysis is a valid tool to evaluate
dairy herds infection prevalence; this mode] is unable to describe all the prevalence variability. Although this
high statistical relationship between UFC log and prevalence, the mode) predictivity performance is to improve
and to use Staph. aureus UFC count in BMT in routine UFC as prediction of cow's infection level within herds
further study have to be run.
ID nr. 3832
Nicholas R, Raedelli E, Luini° M, Loria G, Ayling R, Scanziani E
Prevalence and control of Mycoplasma bovis in Europe
XXV Jubilee World Buiatrics Congress : July 6-11, 2008 Budapest (Hungary) : Oral and poster abstracts /
[s.n. : s. n., 2008]. - 206
Jubilee World Buiatrics Congress (XXV : Budapest (Hungary) : July 6-11, 2008)
Mycoplasma bovis is a major cause of disease in both growing and adult cattle. Pneumonia, mastitis, arthritis
and otitis have all been associated with mycoplasma infection, none of which respond well to chemotherapy.
In the UK serological screening of pneumonic herds indicates that M. bovis is associated with approximately a
quarter of ali outbreaks in calves; this mycoplasma is also the most commonly isolated pathogen from
pneumonic lungs. Since its first introduction into Ireland in the mid 1990s, M. bovis has also become a
frequent isolate from pneumonic lungs. In a recent study of cattle rearing regions in Northern Italy, M. bovis
was the most common bacterial pathogen involved in bovine pneumonia; all veal calves and three quarters of
all beef cattle were seropositive to M. bovis. A commercial vaccine still appears a long way from availability in
Europe but autogenous vaccines, which are being used increasingly, may help to bridge this gap in the short
term.
ID nr. 3833
Radaelli E, Luini° M, Loria GR, Nicholas RAJ, Scanziani E
Bacteriological, serological, pathological and immunohistochemical studies of Mycoplasma bovis
respiratory infection in veal calves and adult cattle at slaughter
Res Vet Sci. - Vol. 85 no2 ( 2008). - p 282-290. - 38 bib ref
Impact factor: 1,274
Pag. 59 di 300 31/08/2011 Mycoplasma bovis is an important cause of calf pneumonia worldwide. In this study, we examined 140 cattle
at slaughter comprising 70 veal calves and 70 beef cattle; 115 animals with pneumonic lesions and 25
without. Lung samples were submitted for bacteriological, histological, and M. bovis-immunohistochemical
analyses. Serology for M. bovis was positive in 76% of beef cattle and 100% of veal calves. M. bovis was
isolated only from veal calves in 16 out of 64 pneumonic cases. M. bovis was detected by
immunohistochemistry in seven bacteriologically positive cases. M. bovis antigen was associated with
bronchogenic necrosuppurative or fibrinonecrotizing lesions. Bacteriologically positive and
immunohistochemical negative cases were associated with catarrhal bronchointerstitial pneumonia. Results
suggest that M. bovis infection may develop into a severe necrosuppurative bronchopneumonia or
fibrinonecrotizing pneumonia when associated with a high number of intralesional organisms or, conversely,
into a mild catarrhal bronchointerstitial pneumonia when associated with a low number of organisms.
ID nr. 3834
Engel B, Buist W, Orsel K, Dekker A, De_Clercq K, Grazioli° S, Van_Roermund Herman
A Bayesian evaluation of six diagnostic tests for foot-and-mouth disease for vaccinated and nonvaccinated cattle
Prev Vet Med. - Vol. 86 ( 2008). - p 124-138. - 21 bib ref
Impact factor: 1,704
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
The sensitivity and specificity of six ELISA tests for foot-and-mouth disease (FMD) to discriminate between
sero-converted (for non-structural FMD virus proteins) and non-sero-converted cattle were evaluated for
vaccinated and unvaccinated cattle. Since none of the tests could be considered as a proper reference test
and for about half of the tested sera the true status (sero-converted or not for non-structural proteins, i.e.
presence of antibodies) of the animals was unknown, a Bayesian analysis employing a latent class model was
used that did not rely on the use of a reference test or gold standard. Prior information about prevalence for
subsets of the data and specificity of the tests was incorporated into the analysis. The specificity of the six
tests for vaccinated and non-vaccinated cattle ranged from 96 to 99%. For vaccinated cattle, one test stood
out with an estimated sensitivity of 94% (95% CI from 89.8 to 98.1 %). Second best for vaccinated cattle were
two tests with estimated sensitivities of 85% (95% CI from 78.9 to 89.7%) and 92% (95% CI from 86.2 to
95.6%). For non-vaccinated cattle, the sensitivities of these three tests were around 97%. The remaining three
tests showed lower estimated sensitivity for vaccinated cattle, ranging from 57 to 79%.
ID nr. 3835
Zarenghi L, Fedrizzi° G, Masselli° M, Caprai° E, Ungari D, Gorreri M, Nocera L, Menotta°
S
Aflatoxin M1 concentration levels inside cheese samples and evaluation of its distribution between
whey and cheese during the production
Sci Tec Latt Casearia. - Vol. 59 no 2 ( 2008). - p 133-139 - 7 ref bib
The contamination from aflatoxins (B and G) in cereals during 2003 caused a massive contamination from
aflatoxin M1 in cow milk and consequently in milk derivates. In Emilia Romagna region the production of
matured cheese is a very important reality, so it was necessary to analyse these products before the
merchandising. These kind of cheese has a long ripening time, its weight is about 30 kg and no informations
were about the aflatoxin M1 distribution inside the cheese. As first step of the work the concentrations of
aflatoxin M1 in different areas of cheese were analysed. Three different cheeses were subdivided in 4 parts
Pag. 60 di 300 31/08/2011 each one from external to internal area (rind, sub-rind, medium area and centre). Each part was analysed
separately and aflatoxin M1 concentration was evaluated. In the same time aflatoxin M1 was analysed from a
portion of total cheese. By means of statistical evaluations it was possible to set the homogeneus distribution
of aflatoxin M1 inside the cheese. The second aim of this work was to know how aflatoxin M1 was transferred
from milk to cheese during the production phases and if it would be possible to evaluate which factors
influence this aflatoxin M1 repartition. The cheeses product from milk with aflatoxin M1 concentration between
0,055 and 0,280 pg/kg had concentrations between 0,080 and 0,640 pg/kg. The calculated transfer factors
were fro 0,7 to 7,7 but 90,7% were lower than 5. No one correlation was detected to explain these differences
from cheese to cheese.
ID nr. 3836
Menotta° S, Caprai° E, Masselli° M, Nocera L, Taus L, Massirio° I, Fedrizzi° G
Emergency during year 2003: control of aflatoxin MI levels in cheese samples produced in Emilia
Romagna region
Sci Tec Latt Casearia. - Vol. 59 no 2 ( 2008). - p 113-119. - 8 ref bib
First author Menotta, Simonetta
During the summer 2003 the particular atmospheric conditions caused the proliferation of aflatoxins (B and G)
in cereals and feed. The resulting massive contamination from aflatoxin M1 in cow milk and derivates cried for
a rapid and exhaustive analysis of all Emilia Romagna products. The Regional Authorities started a particular
plan to improve the controls of regional cheeses produced between November 2003 and December 2004. In
Emilia Romagna region the production of matured cheese is a very important reality, so it was necessary to
analyse theese products before the merchandising. An LC-MS/MS analytical method was developed and 378
cheese samples were analysed. These samples Game from 144 batches of matured cheese that the
veterinary authorities abducted because produced with suspected irregular milk. Totally the 95,2% of samples
was regular with concentration of aflatoxin M1 lower than 0,45 pg/kg. Thirteen batches of cheese were
irregulars: they had one or more cheese with residues of aflatoxin M1 more than 0,450 pg/kg.
ID nr. 3837
Campos RdM, Malirat V, Neitzert E, Grazioli° S, Brocchi° E, Sanchez C., Falczuk AJ, Ortiz
S, Rebello MA, Bergmann IE
Development and characterization of a bovine serum evaluation panel as a standard for
immunoassays based on detection of antibodies against foot-and-mouth disease viral non-capsid
proteins
J Virol Methods. - Vol. 151 no 1 ( 2008). - p 15-23. - 27 bib ref
Impact factor: 1,933
Project SSPE CT 2003 503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
The widespread perception of the effectiveness of applying tests based on the detection of antibodies against
foot-and-mouth disease (FMD) viral non-capsid proteins (NCPs) to assess virus circulation irrespective of
vaccination triggered the demand for international standards to evaluate the comparative performance of the
upcoming assays against the OIE Index test developed at the Pan American Foot-and-Mouth Disease Center,
PAHO/WHO. To this end, a panel was developed composed of 34 cattle sera from animals with an
unambiguous exposed/infected status, covering serotypes 0, A and C, obtained either under experimental
conditions or from the field in regions with different epidemiological situations. Reference values in the Index
test and their reproducibility in other laboratories, data on stability as well as results in four other commercial
kits and one in house test were obtained. The characteristics of the panel which comprise adequate
Pag. 61 di 300 31/08/2011 preparation following international guidelines, a broad range of antibody reactivity, proper stability and the
ability to assess comparative diagnostic sensitivity, make it suitable as a reference standard to evaluate if
tests equivalent to the OIE Index method are used in support of FMD control programs and by trading
partners, and also whether they maintain their standards of diagnostic performance.
ID nr. 3838
Taddei° R, Barbieri° I, Pacciarini° ML, Fallacara° F, Belletti° GL, Arrigoni° N
Mycobacterium porcinum strains isolated from bovine bulk milk : implications for Mycobacterium
avium subsp. paratuberculosis detection by PCR and culture
Vet Microbiol. - Vol. 130 ( 2008). - p 338-347. - 25 bib ref
Impact factor: 2,010
Project PRC2002106 - Development and validation of techniques for detection and viability evaluation of
MAP, a potential hazard for humans, in raw and pastorized bovine milk. - Lillini Dr. Eugenio - UO MINSAN - 2002 - 139150,00 EUR - 15/09/2003 0.00.00 - 30/11/2005 0.00.00 - 1 - N6 Piacenza - Arrigoni Dr.ssa Norma - I
First author Taddei, Roberta
In this study, the isolation of 52 mycobactin-independent fast growing mycobacteria from 631 bulk milk
samples (8.2%), is reported. These strains, isolated during a bulk milk survey for Mycobacterium avium
subsp. paratuberculosis (Map), strongly affected Map detection both by PCR and by culture, as they gave a
positive IS900 PCR signal and resulted to totally inhibit the growth of Map when spotted on HEYM slants
already inoculated with 200 µl of 10-fold dilutions containing from 5 x 10 to 5 x 103 Map cells/ml. 16S rRNA
gene sequencing, using the MicroSeq 500 16S rDNA Bacterial Sequencing Kit (Applied Biosystems), was
performed on a subset of six strains, identifying Mycobacterium porcinum with 100% homology in ali six
cases. The 52 strains were characterized by PCR-restriction fragment length polymorphism (RFLP) analysis
of the hsp65 gene, which confirmed the identification of M. porcinum for al] the isolates. Using specific primers
designed on the Map-IS900 sequence and on the M. porcinum sequence determined in this study, a 1385 bp
sequence from the M. porcinum genome was characterized. This IS900-like sequence showed 82% homology
with Map IS900. From our findings the following results emerged: (a) any culture showing one or more M.
porcinum colonies represents a potential "false negative" result and should therefore be considered as
contaminated; (b) IS900-like elements could be more widespread than was previously thought; (c) IS900 PCR
positive results should be interpreted cautiously, as confirmed by the evidence that the primer pair used in this
study resulted not to be specific.
ID nr. 3840
Ferrari° M, Candotti° P, Lombardi° G, Amadori° M, Dotti S, Guana S, Petrini S
A comparison of the humoral and cell-mediated response of pigs experimentally infected with either
influenza or PRRS viruses
Vet Res Commun. - Vol. 31 suppl 1 ( 2008). - p 199-201. - 5 bib ref
Impact factor: 0,529
Project PRC2004012 - Definition and standardization of immunological parameters for the detection of a
cell-mediated response following influenza virus infection in swine - Amadori Dr. Massimo - Solo MINSAN - 2004 - 32700,00 EUR - 01/07/2005 0.00.00 - 31/12/2006 0.00.00 - 1 - L1 - Reparto
Substrati Cellulari - Ferrari Dr.ssa Maura - I
First author Ferrari, Maura
not found.
Pag. 62 di 300 31/08/2011 ID nr. 3841
Fedrizzi° G, Accurso D, Scandurra S, Montesissa C
Kinetics of Tilmicosin after per os administration to rabbits
Fourth International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM) : Prague, Czech
Republic, August, 24-28, 2008 : program and abstracts / [s.l. : s.n., 2008]. - p 83
International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM) (4 : Prague, Czech
Republic : August, 24-28, 2008)
First author Fedrizzi, Giorgio
Tilmicosin, a macrolide with great clinical efficacy in respiratory diseases, easily attains high intrapulmonary
concentrations as alveolar macrophages can concentrate the drug. When tilmicosin is administered to pigs
(18,5 mg/kg) via drinking water, the drug attains blood levels lower than 0.01 µg/mL and peak concentration in
lungs (0.4-0.6 µg/g) alter 72 hours. In chicks repeat oral tilmicosin (18 mg/kg) attains higher levels in blood
(0.10 pg/ml) and in lungs (3.29 µg/g at 48 hours). In calves fed tilmicosin via milk at 12.5 mg/kg twice a day for
five days, peak concentration was reached in blood 102 h after treatment and in lungs after 78 h (42.7 µg/g).
In the present kinetic study Tilmicosin (12 mg/kg bw) was administered once to 8 fasted rabbits, via oral
gavage. Absorption rate was fast, as blood peak was already attained at the first sampling time (30 min). The
rapid decrease to less than 0.10 µg/ml within two hours and steady blood levels maintained around 0.03 µg/ml
for 48 hours after administration, suggested a rapid distribution to target tissues with a slow release. The
efficacy of orally administered Tilmicosin to control respiratory disease in rabbits was achieved in clinical trials.
A study with 3tilmicosin administered subcutaneously to rabbits, confirmed its fast distribution to lungs: peak
concentration (14.43 µg/g) was attained within two hours and levels >3µg/g were maintained for 72 h. To
confirm that oral Tilmicosin could attain concentrations exceeding the target pathogen's MIC in rabbit lungs
and alveolar macrophages, much faster and longer than in blood, its distribution to target tissues should be
carefully evaluated.
ID nr. 3842
Montesissa C, Zanoni° M, Salogni° C, Guadagnini G, Gradassi° M, Tagliabue° S, Alborali°
L
Five years of in vitro susceptibility testing on Salmonella spp. and E. coli spp strains isolated from
pigs in Italy (2002-2006)
Fourth International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM) : Prague, Czech
Republic, August, 24-28, 2008 : program and abstracts / [s.l. : s.n., 2008]. - p 99
International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM) (4 : Prague, Czech
Republic : August, 24-28, 2008)
First author Zanoni, Maria Grazia
The results on the in vitro susceptibility of 684 and 719 strains of various serotypes of Salmonella spp, and E.
coli spp, respectively, are reported in the present study. All the isolates were collected at farms from pigs
showing enteric symptoms during a five years period (2002- 2006) of routine diagnostic activity. The
antimicrobial susceptibility, of amoxycillin-clavulanic acid and trimethoprim/sulfametazine combinations and
that of aminosidine, kanamicin, apramicin gentamicin, amoxicillin, ceftiofur, cefaloridine, cefquinome,
flumequine, enrofloxacin, danofloxacin, marbofloxacin, florfenicol, tiamulin, tetracyclines, doxicycline, and
sulfadiazine, was assayed by the Kirby-Bauer test. In vitro susceptibility of the E. coli strains, although
variable, was satisfactory. Among drugs frequently employed in pig therapy, the recent combination
amoxicillin-clavulanic acid and the old drug colistine were more effective in vitro than sulphamethazinetrimethoprim or tiamulin (more than 80% were resistant strains. Along the five year study Salmonella spp,
isolates showed lower resistance and variability against the antimicrobial drugs tested, than E coli spp. The
highest percentage of resistant strains was shown against sulfadiazine (94% -100%) and tetracycline (87% 96%), by far greater than that against the sulphametazine-trimethoprim combination (35% -66%). The efficacy
of old but stili used flumequine did not change during the five years study (40%-60%). As expected, the
highest efficacy was shown by the antimicrobials recently authorized for pig therapy and by those marketed as
injectable preparations.
Pag. 63 di 300 31/08/2011 ID nr. 3843
Menotta° S, Carone° V, Bolognesi° E, Caprai° G, Fedrizzi° G
Determination of chloramphenicol residues in royal jelly with liquid chromatography-tandem mass
spectrometry: validation of the method based on 2002/657/EC
Fourth International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM) : Prague, Czech
Republic, August, 24-28, 2008 : program and abstracts / [s.l. : s.n., 2008]. - p 60
International Conference on Antimicrobial Agents in Veterinary Medicine (AAVM) (4 : Prague, Czech
Republic : August, 24-28, 2008)
First author Menotta, Simonetta
During the summer of 2005 some consumer associations accused a massive contamination of
chloramphenicol (CAP) in Royal Jelly imported from China. CAP is a broad-spectrum antibiotic but it's been
included in Annex IV Regulation 2377/90 because its heavy toxically effects demonstrated in human. It's
illegally used against bee's diseases or to preserve honey and its derivates from microbiological
contaminations. Royal jelly, because of its singular properties (restorative characteristics) is consumed by
particular class of people, such as old people and children. To preserve public health, Italian labs began to
analyse this compound, very different from other bees products. The Royal Jelly chemical characteristics
impeded to use screening techniques, so a rapid method for determination and confirmation of CAP was
developed. Following addition of d5-Chloramphenicol as internal standard, Royal Jelly was finely mixed with
silica-powder and extracted with ethyl acetate. After centrifugation, a fraction of supernatant was evaporated.
The residue was reconstituted with methanol-water and analysed in LC-MS/MS. By using an MRM acquisition
method in negative ionization mode, the transitions 321>152, 321>194 and 326>157 were respectively used
for quantification, confirmation and internal standard. The method validation was based on EU-decision
2002/657. The CAP linear range was from 0,1 to 0,6 gg/kg. Intra-laboratory reproducibility was 7,7%:
repeatability was 10%. The mean recovery was 80,6%. CC(alfa) and CCP were 0,14 µg/kg and 0,16 µg/kg
respectively. During the emergency phase (from August 2005 to March 2006) more than 200 samples coming
from China were analysed and 17% presented levels over the MPRL fixed by EC for CAP.
ID nr. 3844
Scaccabarozzi L, Turchetti B, Buzzini P, Pisoni G, Bertocchi° L, Arrigoni° N, Boettcher P,
Bronzo V, Moroni P
Short communication : isolation of Prototheca species strains from environmental sources in dairy
herds
J Dairy Sci. - Vol. 91 n 9 ( 2008). - p 3474 - 3477. - 19 bib ref
Impact factor: 2,361
First author Bertasi, Barbara
Composite milk samples from 548 cows, and samples from feces, feed, bedding, water, liners (before and
after milking), and the postdipping product were aseptically collected from2 ltalian dairy herds from February
to November of 2006. Prototheca. zopfii was isolated from 11.9% of milk samples, 15% of feces. and 33.3%
of bedding samples. No viable cells of P. zopfii were observed in water before washing procedures, whereas
25 to 28.6% of samples from water used for washing both refrigeration tanks and milking equipment were
contaminated with this yeast-like microalga. Analogously, the presence of P. zopfii was detected only on
swabs collected from the liners after milking. Interestingly, in 1 of the 2 herds, water from the drinking trough
was contaminated by viable cells of both P. zopfii and the related environmental species Prototheca
stagn.ora. No viable cells were observed in cow feed. On the basis of the results presented herein, P. zopfir:
seemed to be widespread throughout the environments of dairy herds where outbreaks of bovine mastitis had
occurred.
Pag. 64 di 300 31/08/2011 ID nr. 3851
Camarda A, Circella E, Giovanardi D, Pennelli D, Battista P, Campagnari E, Bruni G,
Tagliabue° S
Avian pathogenic Escherichia coli in Audouin gulls (Larus audouinii) : could they affect the surviving
of the bird colonies?
Ital J Anim Sci. - Vol. 6 no 3 ( 2007). - p 317-320. - 7 bib ref
Impact factor: 0,172
Trentanove stipiti di E. coli isolati da uova embrionale non schiuse e tamponi cloacali di gabbiani corsi (Larus
audouinii), facenti parte di una colonia stanziale nel Salento (Puglia), sono stati sierotipizzati e testati nei
confronti dei principali geni di virulenza espressi dagli Avian Pathogenic Escherichia coli (APEC) (irp2, fyuA,
tsh, papC, fimC, iucD ed eae). Sono stati distinti 8 diversi sierotipi (01, 06, 08, 015, 075, 0139, 0146, 0147).
Elevata la percentuale di E. coli non tipizzabili. La ricerca dei geni di virulenza ha evidenziato la prevalenza
dei geni fimC e irp2 (rispettivamente 94,87 e 97,43%) tipici degli Avian Pathogenic E. coli. I ceppi che
veicolavano il maggior numero di geni di virulenza provenivano soprattutto dalle uova. In particolare, l'8,69%
di questi apparteneva al patotipo FimC/tsh/IucQ1 associato nel pollame a focolai di malattia.
ID nr. 3859
Petrini S, Villa° R, Guercio A, Paniccià M, Ramadori G, Pezzotti G
Study of the possible latency of bovine herpesvirus type 1 (BHV-1) experimentally conferred in
calves previously treated with two BHV-1 DNA vaccines
Atti Soc Ital Sci Vet. - Vol. 62 ( 2008). - cdrom p 143-144. - 5 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (62 : S. Benedetto del
Tronto (AN) : 2008)
Project PRF2003304 - Comparative study of the efficacy of the immunizing systems towards type 1 herpetic
viruses in humans and animals - Guercio Dr.ssa Annalisa - UO - MINSAN - 2003 - 212500,00
EUR - 01/02/2004 0.00.00 - 30/09/2006 0.00.00 - 1 - L1 - Centro Substrati Cellulari - Ferrari
Dr.ssa Maura - I
In this study two DNA vaccines expressing a truncated secreted form of the glycoprotein D (tgD) of BHV-1
and a CpG sequence, were inoculated in calves. Three months after challenge infection with 1311V-1 virulent
strain, all calves were subjected to dexamethasone (DMZ) treatment, for the study of latency. No clinical signs
of disease were observed in any of the inoculated calves during the observation period. The virulent virus was
reactivated from all calves 7 and 9 days post treatment with DMZ. The neutralizing antibody against BHV-1,
and ELISA antibody against tgD of BHV-1 increased 24 days post treatment with DMZ. On the basis of the
results obtained, the DNA vaccines used in this experiment, failed to protected the calves against the latency
established with BHV-1 virulent strain.
ID nr. 3860
Corradi A, Losi° CG, Renzi° S, Sesso L, Galdi A, Motta A, Cinotti° S
Isolation, amplification and characterization of equine chondrocytes
Atti Soc Ital Sci Vet. - Vol. 62 ( 2008). - cdrom p 179-180. - 5 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (62 : S. Benedetto del
Tronto (AN) : 2008)
Use of Mesenchymal Stem Cells (MSCs) represents an approach in tissue reconstitution, thanks to their
ability to differentiate in various cells of the mesodermal line, including cartilage. An alternative system
consists in cell therapy using autologous chondrocytes. These cells could be used in tissue engineering and
Pag. 65 di 300 31/08/2011 represent a reference cell system for MSCs based treatment. In the present study, equine chondrocytes were
cultivated in vitro; furthermore inclusion in alginate beads, solubilisation and characterization were performed.
In future, MSCs plasticity and growth of this type of cells on different bioscaffolds will be evaluated, together
with the analysis in immunohistochemistry and immunofluorescence.
ID nr. 3864
Decaro N, Desario C, Miccolupo A, Campolo M, Parisi A, Martella V, Amorisco F, Lucente
MS, Lavazza° A, Buonavoglia C
Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis
J Gen Virol. - Vol. 89 no 9 ( 2008). - p 2290-2298. - 35 bib ref
Impact factor: 3,120
Thirty-nine parvovirus strains contained in faecal samples collected in Italy (n=34) and UK (n=5) from cats
with feline panleukopenia were characterized at the molecular level. All viruses were proven to be true feline
panleukopenia virus (FPLV) strains by a minor groove binder probe assay, which is able to discriminate
between FPLV and the closely related canine parvovirus type 2. By using sequence analysis of the VP2 gene,
it was found that the FPLV strains detected in Italy and UK were highly related to each other, with a nucleotide
identity of 99.1-100 and 99.4-99.8% among Italian and British strains, respectively, whereas the similarities
between all the sequences analysed were 98.6-100%. Eighty-eight variabie positions were detected in the
VP2 gene of the fieid and reference FPLV strains, most of which were singletons. Synonymous substitutions
(n=57) predominated over non-synonymous substitutions (n=31), and the ratio between synonymous and
non-synonymous substitutions (dN/d S) was 0.10, thus confirming that evoiution of FPLV is driven by random
genetic drift rather than by positive selection pressure. Some amino acid mutations in the VP2 protein affected
sites that are thought to be responsible for antigenic and biological properties of the virus, but no clear
patterns of segregation and genetic markers, were identified, confirming that FPLV is in evolutionary stasis.
ID nr. 3865
Zanoni° M, D'Incau° M, Spisani° M, Gaffuri° A, Tagliabue° S, Alborali° L
Isolation and characterization of salmonella strains from wild boar (Sus scrofa) in Northern Italy
8th conference of the European Wildlife Disease Association (EWDA) : focusing on diseases of European
wildlife and recent changes in disease distribution : 2-5 October, 2008 Rovinj, Croatia / [s.l. : s.n., 2008]. - p
95-96
Conference of the European Wildlife Disease Association (EWDA) (8th : Rovinj, Croatia : 2-5 October,
2008)
First author Zanoni, Maria Grazia
In order to investigate the role of wild boar as a reservoir of Salmonella spp. we controlled, during the 2006
and 2007 hunting season, 786 animals from a mountain area in Brescia Province, Northern Italy. Cecal
content were sampled and cultured according To ISO 6579: 2002 Annex D and, at the same time, To an
home made method using Rappaport Vassilidais Broth as enrichment and Hektoen Enteric Agar as differential
selective medium. 217 samples (27,61%) resulted positive showing that the most prevalent serotypes,
covering 76,03% of the positive isolates, were Salmonella Coeln (45 isolates) Salmonella Ball (34),
Salmonella Typhimurium (29), Salmonella enterica subsp. diarizonae 50:r:1,5J71 (23), Salmonella Thompson
(19) and Salmonella Veneziana (15). Among the other serotypes, 4 isolates of Salmonella Enteritidis were
recovered. Phagetyping of Salmonella Typhimurium revealed the prevalence of phagetype DT104 (17
strains), DT193 (2), U302 (2), DT2 (1); among Salmonella Enteritidis, the only phagetype present was PT4 (3
strains). Strains of Salmonella Typhimurium were also tested for antimicrobial resistance using a panel of 16
antibiotics: 25 strains resulted multi - resistant (resistant to 4 or more antibiotics) and among them, all
phagetype DT104 strains were resistant from 6 To 10 antibiotics with a basic profile of resistance for
amoxicillin, chloramfenicol, spectinomycin, streptomycin, sulphonamides and tetracycline. Strains of
Salmonella Enteritidis did noi show multiresistance against tested antibiotics.
Pag. 66 di 300 31/08/2011 ID nr. 3866
Gaffuri° A, Monaci° C, Testa E, Bosio G, Pisoni L, Moroni G, Paterlini° F
Wildlife health monitoring in Bergamo Province, Northern Italy: a coordinated program between the
official veterinary service, the public administration and the hunters
8th conference of the European Wildlife Disease Association (EWDA) : focusing on diseases of European
wildlife and recent changes in disease distribution : 2-5 October, 2008 Rovinj, Croatia / [s.l. : s.n., 2008]. - p
95-96
Conference of the European Wildlife Disease Association (EWDA) (8th : Rovinj, Croatia : 2-5 October,
2008)
First author Gaffuri, Alessandra
Wildlife welfare is a common concern of different stakeholders: the official veterinary service, for the
increasing number of infectious diseases shared between wildlife, domestic animals and humans, the public
administrators, for management reasons, and the hunters, for a direct interest in hunter-harvesting. Since
1997 a health monitoring on wildlife in Bergamo Province has been performed, with the informal cooperation
of the hunters associations. In 2005 an agreement between the official veterinary service, the public
administrators and the hunter associations formalised the collaboration between these parties, with the
common aim to a better understanding of health and disease in freeranging wildlife. Through this accord the
biological samples, such as blood, faeces and viscera, are collected by the hunters during the hunting
seasons and delivered to the laboratory for analysis. Moreover dead animals, recovered in the field by
gamekeepers or by hunters, are submitted for necropsy to investigate the death's cause. This network allows
to check wildlife morbidity and mortality and to recognize atypical events in the most important wild species of
the Province: roe deer, red deer, chamois and wild boar. The presence of some infectious diseases has been
investigated through the yearly monitoring program, in particular Brucellosis, Trichinellosis, Classical Swine
Fever, Swine Vesicular Disease, Tuberculosis, Johne's disease, Lyme disease, Respiratory Syncytial Virus,
Infectious Keratoconjunctivitis, Salmonellosis. Right now we have historic data that can be useful to define the
sanitary picture in the wildlife of our Province. Interesting data are recovered by the analysis of both hunted
and dead animals; for instance we assessed the presence of M. microti in tuberculosis-like lesions in the wild
boar population, we studied the circulation of Respiratory Syncytial Virus inside the chamois population and
we have been checking through the years for the potential presence of relevant zoonosis, such as Brucellosis
and Trichinellosis.
ID nr. 3867
Gaffuri° A, Monaci° C, Vicari° N, Paterlini° F, Magnino° S
Detection of Chlamydophila pecorum in the lung of an Alpine chamois (Rupicapra rupicapra) in
Northern Italy
8th conference of the European Wildlife Disease Association (EWDA) : focusing on diseases of European
wildlife and recent changes in disease distribution : 2-5 October, 2008 Rovinj, Croatia / [s.l. : s.n., 2008]. - p
60
Conference of the European Wildlife Disease Association (EWDA) (8th : Rovinj, Croatia : 2-5 October,
2008)
First author Gaffuri, Alessandra
Chlamydial infections have been widely detected worldwide both in farmed and in free-living animals,
including wild ruminants. Several species belonging to the family Chlamydiaceae cause major reproductive
disease (metritis, infertility and abortion), conjunctivitis, arthritis, encephalomvelitis and pneumonia. In our
study area, a mountain region in the province of Bergamo, Northern Italy, two causes of Chlamydophila
abortus infection were detected in the last few years respectively in a mummified roe deer foetus and in
individuals of an ibex population affected by conjunctivitis. Moreover, Chlamydophila pecorum has been
isolated several years ago in a chamois affected with pneumonia in another area of the Alps. We report here
Pag. 67 di 300 31/08/2011 the detection of Chlamydophila pecorum in a 7 month-old female chamois, submitted to the laboratory by a
gamekeeper. The animal came from an area where a remarkable mortality in kids was occurring. At necropsy,
the chamois presented a nasal discharge with conglutination of the hair of the nasal region. A fibrinous
pericarditis and pleuritis was observed; the lungs were enlarged, oedematous and showed areas of red
consolidation in the cranial lobes and at the peripheral sites of the middle and caudal lobes. Wide areas of
parasitic nodular lesions were observed in the caudal lobes. Laboratory investigations for bacteria, including
mycoplasmas, were performed by inoculating samples of lungs onto Blood agar and PPLO agar plates. Only
Moraxella sp., but no mycoplasma, were isolated. Specific PCR-RFLP targeting chlamydiae was carried out
and allowed to detect Chlamydophila pecorum. The virological investigation focused on the research of a
respiratory syncytial virus, which has been recognized as an agent of pulmonary distress in the area of
investigation, but the lung tested negative. No information other than a marked decrease in the number of kids
in the chamois population was reported by the gamekeeper nor by local hunters. Although the role of
Chlamydophila pecorum and Moraxella in determining the death of the kids is unknown, it is suggested that
the necropsied chamois had died of acute respiratory distress, caused by Chlamydophila pecorum with the
superinfection by Moraxella sp..
ID nr. 3868
Arrigoni° N, Belletti° G, Manini° F, Galletti° G, Gelmini° L, Merialdi° G, Renzi° M, Tamba°
M
Survey on the prevalence of paratuberculosis in the Emilia-Romagna Region by bulk milk ELISA test
Buiatria. - Vol. 3 no 2 ( 2008). - p 3-9. - 9 bib ref
Project PRC2005003 - Use of bulk-milk for bovine paratuberculosis surveillance - Arrigoni Dr.ssa Norma Solo - MINSAN - 2005 - 30000,00 EUR - 02/05/2006 0.00.00 - 01/05/2008 0.00.00 - 1 - N6 Piacenza - Arrigoni Dr.ssa Norma - I
2 - N0 - CEREV - Tamba Dr. Marco - I
First author Arrigoni, Norma
By using a commercial ELISA test (Institut Pourquier), a survey on 5,081 bulk milk samples belonging to
3,600 dairy herds of the Emilia Romagna region (74.2% of the total herds) has been performed. The herds
were submitted to a variable number of tests. On the basis of a single sample per herd, the apparent
prevalence of infected herds is 3.2% (95% c.l: 2.7-3.9%), while the estimated real prevalence is 10.7% (95%
c.l.: 9.7% - 11.8%). In some provinces, using repeated tests (3 samples/herd), the prevalence resulted
significantly higher than the prevalence estimated on the basis of a single test. To obtain an accurate
prevalence estimate, the use of a scheme based on repeated sampling is preferable, giving more
conservative results.
ID nr. 3869
Arrigoni° N, Cammi° G, Cerutti° G, Manini° F, Bersani A, Cavalli L, Fava M, Fea C, Fumi
E, Guareschi F, Zioni O, Belletti° GL
Study on the persistence of Mycobacterium avium subsp. paratuberculosis in crop fertilized with
slurry from bovine infected herds
Buiatria. - Vol. 3 no 2 ( 2008). - p 11-16. - 7 bib ref
First author Arrigoni, Norma
A study on the persistence of Mycobacterium avium subsp. paratuberculosis (Map) in crop fertilized during the
autumn-winter period with slurry or manure coming from bovine infected herds with different prevalence levels
of Paratuberculosis, was carried out. The tests performed on the crop samples, collected before harvesting,
showed a single positive result by PCR (10%) and were always negative to culture. The crop samples,
collected after harvesting and at the beginning of their use for animal feeding, were always negative, both in
culture and in PCR. These results lead us to conclude that, in the described conditions, the contamination risk
for crop, although possible, is of limited importance for the spreading of infection, compared with other
Pag. 68 di 300 31/08/2011 hygienic and managerial factors.
ID nr. 3870
Bertocchi° L, Varisco° G, Bolzoni° G, Fedrizzi° G, Rumi S
Preliminary observations on presence of PCDD/F and dl in cow's milk
Buiatria. - Vol. 3 no 2 ( 2008). - p 17-23. - 7 bib ref
First author Bertocchi, Luigi
Purpose of this work is to highlight the level and type of contamination by organochlorine compounds; PCDD
(polychlorinated dibenzo-p-dioxin), PCDF (polychlorinated dibenzofurans) and dl-PCB (polychlorinated dioxin
like) of milk tanker composed by diaries bulk milk of Brescia and hinterland. In this area, in the past industries
had produced PCBs (1) and still today it's site of many ironworks and steelworks;pollution problem is known
and it was monitored , but, the application of new regulations (Reg. CE n° 1881/2006 and Reg. CE n°
1883/2006) ori the limits and the composition of contaminants had imposed new epidemiological studies to
provide data and tolls for risk evalutation and analysis. Milk tanker analysis showed WHO-PCDD/F PCB-TEQ
values between 2,589 and 3,03 pg/gr of fat, lower than legal limit (6,00 pg/gr. of fat) but higher than action
limit 2,00 pg/gr. of fat (raccomandazione CE 144/06) The results indicate PCB as primarily contamination
compuonds , representing more than 65% of pollutants, lower than dioxins (PCDD/F). Food for human
consumption environmental contamination is certainly widespread, given the huge dispersion of these
substances into environment, but there are a few epidemiological studies that describe the magnitude and
type of risk.
ID nr. 3871
Tosi° G
Welfare of alternative poultry breeds and free-range systems
30 giorni. - Vol. 1 no 8 ( 2008). - p 101-102
First author Tosi, Giovanni
In this review the standards for the inspection of welfare of the following production systems are described:
the rearing production of local poultry breeds and the free-range systems.
ID nr. 3872
Bertocchi° L
Welfare in dairy and beef cattle
30 giorni. - Vol. 1 no 8 ( 2008). - p 46-48
First author Bertocchi, Luigi
This study evaluates welfare on cattle through the combination of observations and findings. These methods
should include measurements of behaviour, health status, productive performances of quantity and quality of
milk and meat and finally of environmental parameters (stalls, litter and the micro-climate). Here we have
shown the principal findings and measurements needed for a full appreciation of cattle welfare.
ID nr. 3874
Rota Nodari° S
The legislative decree 146/2001
Pag. 69 di 300 31/08/2011 30 giorni. - Vol. 1 no 8 ( 2008). - p 22-24
First author Rota_Nodari, Sara
The author illustrate the content of the legislative decree 146/2001 on the welfare of the animals kept for
farming purposes..
ID nr. 3876
Vinco° JL
Turkeys
30 giorni. - Vol. 1 no 8 ( 2008). - p 94-100
First author Vinco, James Leonardo
Current animal welfare legislation does not cover turkeys in specific in spite of the large consumption and
production of turkey meat in Europe. This paper highlights the critical points related to the welfare of turkeys
at farm level .The ultimate goal is to provide guidelines to the official veterinarians involved in carrying out
welfare audits in turkey farms in order to comply with Directive 98/58 CE.
ID nr. 3877
Lavazza° A, Cerioli° M, Grilli G
Animal Welfare on Farms: application of the National Law 146/2001 in rabbits and hare farms
30 giorni. - Vol. 1 no 8 ( 2008). - p 103-118
First author Lavazza, Antonio
In Italy, the protection of animals including, fish, reptiles and amphibians that are reared for food stuffs, wool,
skins, furs or any other agricultural purpose, is regulated by law n. 146/2001, governed by directive 98/58/EC
and by the specific laws referring to catttle, swine and layer farms.
This chapter specifically deals with rabbit and hares farming. It met the requirement of the whole course i.e. to
improve national animal awareness, so that consumer ethical requirements meet the equally valid
expectations of farm development and in particular to give farmers the educational and informational basis to
respect community regulations.
ID nr. 3880
Bosi P, Casini L, Tittarelli° C, De_Filippi S, Trevisi P, Clavenzani P, Mazzoni M
Effect of dietary addition of nitrate on growth, salivary and gastric function, immune response, and
excretion of Salmonella enterica serovar Typhimurium, in weaning pigs challenged with this microbe
strain
Ital J Anim Sci. - Vol. 6 suppl 1 ( 2007). - p 266-268. - 7 bib ref
Impact factor: 0,172
Two dietary additions of nitrate (15 mg/kg or 150 mg/kg, supplied by potassium salt) were test-ed in a total 96
weaning pigs challenged or not with Salmonella enterica serovar typhimurium (ST). The oral challenge was
done on d 5 and pigs were sacrificed on d 7 or d 25. The effect of challenge never interacted significantly with
the dietary treatment. Feed intake; growth, body temperature, salivary excretion, and faecal excretion of ST
and gastric function were not affected by the nitrate supplementation. With nitrate additions, total IgA in blood
serum tended to be higher before and after the challenge (P<0.10). Nitrite in saliva - but not nitrate - increased
with the increasing supplementation at d 5, but not at d 19. The nitrate additions did not negatively affect the
weaning performance, but also did not contrast the effect of ST infection.
Pag. 70 di 300 31/08/2011 ID nr. 3886
Vitali A, Bertocchi° L, Lacetera N, Bernabucci U, Cuteri A, Guerini° M, Nardone A
Seasonal variation of mortality rate in dairy cows of the Po Valley (Italy) : a retrospective study from
2001 to 2006
J Anim Sci. - Vol. v 86 e-suppl 2 ( 2008). - p 2
Joint Meeting American Dairy Science Association American Society of Animal Science : July 7 -11, 2008)
Impact factor: 2,102
The present study is aimed to analyze seasonal variations of mortality rate in dairy cows. The analysis was
carried out in years 2001-2006 and in the geographic area comprised between 44°- 46°.15' latitude north and
8 '.30'- 12°.30' longitude east, known as the area of the Po Val ley including the regions Lombardia and Emilia
Romagna, Italy. This area is characterized by a subcontinental-temperate climate and a high density of dairy
herds (approximately 900,000 dairy cows in 45,000 square kilometres). Data were extracted from the Italian
Bovine Spongiform Encephalopathy (BSE) database, which provided data of daily mortality of cows older than
24 months. Data on cow populations were provided by the ltalian National Institute of Statistics. The
standardized mortality ratio (SMR) for each season and for each year was calculated by the ratio of observed
and expected deaths (OD and ED, respectively). The ED for each of the 6 years considered in the study were
calculated by the product between annual mortality rate and the population of the area, stratified by 4 age
classes. Under the hypothesis of Poisson distribution, a 95% confidence interval (CI) was calculated for the
SMR and its value was considered statistically significant if the valve 1 was outside the confidence range. For
all years and regions, the analysis of SMR showed that during summer season the OD was significantly
higher than the ED. In summer season OD overcame ED by values ranging from +21% (year 2005.
Lombardia) to +60% (year 2003. Lombardia), the corresponding 95% CI were 1.17-1.24 and 1.57-1.64 for the
years 2005 and 2003, respectively. Results reported herein indicate the relevance to develop appropriate
strategies, including management, feeding, genetic selection and insurance plans, to limit economic losses
associated with heat stress in dairy cows.
ID nr. 3886a
Vitali A, Bertocchi° L, Lacetera N, Bernabucci U, Cuteri A, Guerini° M, Nardone A
Seasonal variation of mortality rate in dairy cows of the Po Valley (Italy) : a retrospective study from
2001 to 2006
J Dairy Sci. - Vol. v 91 e-suppl 1 ( 2008). - p 2
Joint Meeting American Dairy Science Association American Society of Animal Science : July 7 -11, 2008)
Impact factor: 2,361
The present study is aimed to analyze seasonal variations of mortality rate in dairy cows. The analysis was
carried out in years 2001-2006 and in the geographic area comprised between 44°- 46°.15' latitude north and
8 '.30'- 12°.30' longitude east, known as the area of the Po Val ley including the regions Lombardia and Emilia
Romagna, Italy. This area is characterized by a subcontinental-temperate climate and a high density of dairy
herds (approximately 900,000 dairy cows in 45,000 square kilometres). Data were extracted from the Italian
Bovine Spongiform Encephalopathy (BSE) database, which provided data of daily mortality of cows older than
24 months. Data on cow populations were provided by the ltalian National Institute of Statistics. The
standardized mortality ratio (SMR) for each season and for each year was calculated by the ratio of observed
and expected deaths (OD and ED, respectively). The ED for each of the 6 years considered in the study were
calculated by the product between annual mortality rate and the population of the area, stratified by 4 age
classes. Under the hypothesis of Poisson distribution, a 95% confidence interval (CI) was calculated for the
SMR and its value was considered statistically significant if the valve 1 was outside the confidence range. For
all years and regions, the analysis of SMR showed that during summer season the OD was significantly
higher than the ED. In summer season OD overcame ED by values ranging from +21% (year 2005.
Lombardia) to +60% (year 2003. Lombardia), the corresponding 95% CI were 1.17-1.24 and 1.57-1.64 for the
Pag. 71 di 300 31/08/2011 years 2005 and 2003, respectively. Results reported herein indicate the relevance to develop appropriate
strategies, including management, feeding, genetic selection and insurance plans, to limit economic losses
associated with heat stress in dairy cows.
ID nr. 3894
Porrini C, Sabatini AG, Mutinelli F, Astuti M, Lavazza° A, Piro R, Tesoriero D, Medrzycki P,
Sgolastra F, Bortolotti L
Cases of depopulation and mortality in apiaries in Italy: report of investigation conducted during
2008
Osservatorio. - Vol. 11 no 6 ( 2008). - p 13-15
During last years several cases of bee losses have been reported during the period of corn sowing in different
European countries. During 2008 when an increase of cases was reported, some Italian regions decided to
organise an official network to collect data and analyse dead bee samples. The visual examination and the
virological analyses excluded pathological causes. The spatial and temporal correlation between hive
damages and corn sowing and the presence of residues of active ingredients used for seed dressing
(imidacloprid, thiamethoxam and clothianidin) in almost half of the samples confirm the connection between
Spring mortality and the sowing of corn seed dressed with neonicotinoids.
ID nr. 3895
Massi° P, Tosi° G, Fiorentini° L
Experimental challenge trial with a “very virulent” strain of infectious bursal disease virus (vvIBDV)
in commercial pullets vaccinated with an IBD vectored vaccine or with three different modified live
vaccines
Zootec Int. - Vol. 11 ( 2008). - p 50-57. - 6 bib ref
First author Massi, Paola
An experimental challenge trial with a “very virulent” strain of infectious bursal disease virus (vvIBDV) was
carried out in commercial pullets with maternally derived antibodies to IBDV and reared in isolators. The aim
of the study was to compare the protection induced by the IBD vector vaccine vHVT13, in which the turkey
herpesvirus (HVT) is used as the vector of the IBDV VP2 gene, with the protection induced by different
conventional live vaccines.
ID nr. 3897
Zanoni° M, Guadagnini G, Gradassi M, Salogni° C, D'Incau° M, Alborali° GL
Study of antibiotic resistance in Salmonella typhimurium strains isolated during 2005-2007
20th International Pig Veterinary Society Congress (IPVS) : Durban, South Africa 22-26 June 2008 : Poster
Volume 2 / Durban, South Africa : Hein Jonker Media Managment, 2008. - p 503 - 5 bib ref
International Pig Veterinary Society Congress (IPVS) (20 : Durban, South Africa : 22-26 June 2008)
First author Zanoni, Maria Grazia
not found.
ID nr. 3898
Pozzi SP, Yadin H, Lavi J, Pacciarini° M, Alborali° L
Pag. 72 di 300 31/08/2011 Porcine circovirus type 2 (PCV2) infection of pigs in Israel : clinical presentation, diagnosis and virus
identification
Isr J Vet Med. - Vol. 63 no 4 ( 2008). - p 122-124. - 14 bib ref
Impact factor: 0,105
During the years 2006-2007, an increase of respiratory and enteric problems in piglets after weaning was
reported from pig herds in westem Galilee. These problems were accompanied by increased mortality and a
lower growth rate of the affected piglets. A reduced growth rate was also noted in older pigs at these farms.
Sick piglets were bled on three affected farms, and on necropsy, lungs, inguinal and mesenteric lymph nodes
were collected from carcasses, and submitted to laboratory examination. Evidence for the presence of porcine
circovirus type 2 (PCV2), was based on positive ELISA serology; virus isolation; polymerase chain reaction
(PCR) and immunohistochemistry (IHC) on the lymphoid tissues. To our knowledge this is the first case on
PCV2 identification in Israel pigs.
ID nr. 3900
Franco A, Merialdi° G, Lurescia M, Feltrin F, Buccella C, Riccardi F, Bassoli O, Cito G,
Cuoghi G, De_Bassa E, Floriani E, Perrone V, Razzini P, Liuzzo G, Battisti A
Survey for methicillin resistant Staphylococcus aureus in slaughter pigs in Italy. Preliminar report
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 102-103
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
First author Merialdi, Giuseppe
Preliminar results of a MRSA survey in pigs at slaughterhouse are reported. The study included batches from
118 fattening herds. Sixty pigs from each batch were sampled by nasal swab. Thirty-eight percent of holdings
resulted colonized. Genetic heterogeneity between isolated strains was shown. More complete genetic
characterization will be reported in an exhaustive paper.
ID nr. 3901
Delogu M, Ostanello F, Moreno_Martin° A, Lelli° D, Frasnelli° M, Marzadori° F, Raffini° E,
De_Marco MA
PCV2 infection in wild boar: antibody dynamics in a monitored population in a protected area (20022006)
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 91
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
PCV2 antibody dynamics was studied in a demographic managed wild boar population in Northern Italy from
2002 to 2006. A total of 1499 sera were tested with a competitive ELISA assay. Serological prevalence was
determined in 4 age classes (< 6 months, 6-12 months, 1-2 years, > 2 years) and in sex classes. Higher
prevalence were found in females with a statistically significant difference and in age classes 1 and 4 .
Antibody titres were higher in adult (classes 3 and 4) than in young animals..
Pag. 73 di 300 31/08/2011 ID nr. 3902
Tamba° M, Venturi L, Angelini P, Baldelli R, Bellini R, Borrini BM, Dottori° M, Poglayen G,
Rugna° G, Venturelli C, Martini E
Surveillance on vector-borne diseases in Emilia-Romagna Region, Italy
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 151-152
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
First author Tamba, Marco
In recent years an increase in vector-borne disease activity has been recorded in different regions. The
epidemiology of such diseases is conditioned by complex interactions among environment, parasite, vector,
man and eventually animal - either domestic or wild - reservoir. For these reasons a regional surveillance
program focused on vectored diseases was implemented in Emilia-Romagna. The aim of the project is to
create a regional surveillance and risk assessment system based on a multidisciplinary network, with the
capability of collecting data about both vector populations dynamics, and possible presence of pathogens in
vectors, men and animals. It has therefore been constituted a working group composed by physicians,
veterinarians and entomologists. After only two months from its constitution, the working group had to manage
the Chikungunya epidemic occurred in Ravenna and Cervia municipalities during summer of 2007. After only
15 days from the reporting of the first human cases, it has been possible to perform the diagnosis, isolating
the virus from the vector (Aedes albopictus), to define a reporting system of clinical suspects and diagnostic
procedures in man. In the same time an intensive vector control program was carried out in infected areas..
ID nr. 3903
Spaggiari° B, Cuccurese A, Merialdi° G, Aldrovandi A, Massirio I, Leonelli° R, Dottori° M
Survey of pathogen diffusion in a roe deer (Capreolus capreolus) population affected by diarrhoea in
the province of Reggio Emilia.
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 148-149
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
First author Spaggiari, Brunella
A survey was conducted between 2007 and 2008 on free-living roe deers in the province of Reggio Emilia
following a reported increased mortality in the population during summer 2007. Enterocolitis, which was found
to be the preeminent cause contributing to roe deer wasting and death, along with high roe deer densities and
unfavourable environmental conditions, has concurred to the lethal outcome for a considerable number of
animals.
ID nr. 3904
Pongolini° S, Bergamini° F, Carra° E, D'Incau° M, Lambertini A, Fontana R, Bassi° S
Use of genetic markers for epidemiological investigation of food-borne outbrakes by Salmonella
enterica subsp. enterica serovar Enteritidis
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 136-137
Pag. 74 di 300 31/08/2011 Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
Project PRC2005006 - Study of the virulence, sensitivity to antibiotics and genetic characteristics of strains
of Salmonella enterica sub. Enterica isolated from avian species, particularly regarding serovars of
high significance to public health - Tosi Dr. Giovanni - Solo - MINSAN - 2005 - 20000,00 EUR 03/04/2006 0.00.00 - 02/04/2007 0.00.00 - 1 - N3 - Forlì - Massi Dr.ssa Paola - I
2 - N4 - Modena - Pongolini Dr. Stefano - I
First author Pongolini, Stefano
The aim of the study was the molecular epidemiological investigation of two unrelated food-borne outbrakes
caused by Salmonella enterica subsp. enterica serovar Enteritidis that took place in the Province of Modena in
2007. A total of 14 isolates were obtained from patients, food, food products and farm animals involved in the
suspected food chains (eggs and turkey meat). MLVA and PFGE assay of the isolates confirmed their identity
within each episode and permitted to distinguish the strains involved in the two unrelated episodes. In
particular, MLVA with eight markers showed superior performance, precisely discriminating the two strains..
ID nr. 3905
Tamba° M, Rugna° G, Baldelli R, Calzolari° M, Dell'Anna° S, Dottori° M, Galletti° G, Piva
S, Poglayen G, Renzi° M, Martini E
Surveillance on Canine Leishmaniosis in dog shelters of Emilia-Romagna
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 155-156
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
First author Tamba, Marco
Canine Leishmaniosis (Lcan) occurrence is raising in Northern Italy causing an increase in human cases. In
2007 a surveillance program in dog shelters started in Emilia-Romagna with the aim to control Lcan. In 22 dog
shelters both serological and entomological program were carried out. Over 3000 dogs were tested by IFI with
an overall prevalence of 1.5%. Vector presence was detected trough sticky traps in 7 sites. Shelters were
classified in four risk classes based on presence/absence of vector (Ph.perniciosus or Ph.perfiliewi) and
presence/absence of infected dogs (IFI titer >1:80). Different preventive measures were defined and
implemented for each risk class.
ID nr. 3906
Tamba° M, Fontana MC, Leonelli° R, Santi° A, Martini E, Barigazzi° G, Bardasi° L,
Dottori° M
Yersinia enterocolitica O:9 and false positive reactions to Bovine Brucellosis serological tests
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 153 -154
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
Project PRC2005007 - Study of the unspecific reactions in the diagnosis of bovine brucellosis - Dottori Dr.
Michele - Solo - MINSAN - 2005 - 109600,00 EUR - 05/06/2006 0.00.00 - 04/06/2008 0.00.00 1 - N8 - Reggio Emilia - Dottori Dr. Michele - I
2 - G2 - Reparto di Batteriologia Specializzata - D'incau Dr. Mario - I
3 - N4 - Modena - Barigazzi Dr. Giuseppe - I
4 - N0 - CEREV - Tamba Dr. Marco - I
5 - T1 - OEVRL - Zanardi Dr. Giorgio - I
Pag. 75 di 300 31/08/2011 6 - M2 - Bergamo - Paterlini Dr. Franco - I
First author Tamba, Marco
Yersinia enterocolitica (YE) O:9 causes false positive reactions to Bovine Brucellosis serological tests. A
survey on YE prevalence was carried out in cattle in Emilia-Romagna. Between may 2006 and april 2008
1939 single faeces samples were collected and tested for YE presence. Cattle tested were divided in two
groups: (A) heads positive to Brucellosis complement fixation test (n. 151), and (B) heads negative to
Brucellosis complement fixation test (n. 1290) YE was detected in 72 animals. YE prevalence were equal to
35.1%in group A and equal to 1.5% in group B. 100% of YE isolated in group A and 42% in group B were
identified as O:9. In Emilia-Romagna YE O:9 seems strictly related to false positive reactions to brucellosis
test.
ID nr. 3907
Bardasi° L, Fontana° MC, Stanzani F, Marliani A, Palminteri S, Baldi M, Grandini S,
Rugna° G, Galletti° G, Merialdi° G, Vecchi° G
Survey on Dermatophytes diffusion in feline colonies in Bologna between 2004-2007
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 55-56
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
First author Bardasi, Lia
Dermatophytoses are common infections of domestic animals. Among domestic species dogs and cats show
the highest levels of prevalence. Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes
are the main etiological agents of clinical dermatophytoses. Between January 2004 and December, 2007,
dermatological specimens (hairbrush) from 2572 cats of feral colonies living in city of Bologna were examined
for Dermatophytes. 278 (10,8%) examined animals scored positive for dermatophytes. M. canis was the most
frequently recovered dermatophyte.
ID nr. 3909
Tamba° M, Arrigoni° N, Galletti° G, Gelmini° L, Merialdi° G, Renzi° M, Belletti° G
Comparison between different sampling methods to estimate Johne's disease prevalence trough
ELISA on bulk milk
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 24-25
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
Project PRC2005003 - Use of bulk-milk for bovine paratuberculosis surveillance - Arrigoni Dr.ssa Norma Solo - MINSAN - 2005 - 30000,00 EUR - 02/05/2006 0.00.00 - 01/05/2008 0.00.00 - 1 - N6 Piacenza - Arrigoni Dr.ssa Norma - I
2 - N0 - CEREV - Tamba Dr. Marco - I
First author Tamba, Marco
To estimate Johne's disease herd prevalence in Emilia-Romagna two sampling methods were compared. In
782 herds during the same year bulk milk were sampled both one and three-time and tested through
commercial ELISA. Apparent prevalences were corrected by sensitivity and specificity of the used test. The
estimated prevalence resulted from three-time sampling method was significantly higher than prevalence from
single sampling. Due to low test sensitivity, repeated sampling is needed to properly estimated herd
Pag. 76 di 300 31/08/2011 prevalence.
ID nr. 3910
Bertoletti° I, Bianchi° A, Andreoli E, Mattiello S
Sanitary survey of wild roe deer (Capreolus capreolus) hunted in Sondrio Province (Italy) during
2006
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 57-58
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
First author Bertoletti, Irene
To investigate the health conditions of wild roe deer (Capreolus capreolus) in Sondrio Province (Central Italian
Alps), gross necropsies on 34 roe deer found dead in field and 42 roe deer culled during three hunting
seasons (1st September-15th december) – years 2006, 2007 and 2008 - were carried out collecting blood,
faecal and tissue samples.
ID nr. 3911
Andreoli E, Bertoletti° I, Bianchi° A, Radaelli E, Scanziani E, Mattiello S
Serological and istological survey about Leptospirosis in population of red deer (Cervus elaphus) in
Central Alps
Epidemiologia : strumenti per conoscere, agire e decidere in sanità pubblica veterinaria : IV Workshop
nazionale di epidemiologia veterinaria : Università degli studi "Sapienza" : Roma, 11-12 Dicembre 2008 :
riassunti / a cura di Gaia Scavia, Susan Babsa e Marcello Sala. - Roma : Istituto Superiore di Sanità, 2008. (ISTISAN congressi ; 08/C12) p 49-50
Workshop nazionale di epidemiologia veterinaria (4 : Roma : 11-12 Dicembre 2008)
To investigate the prevalence of Leptospirosis in wild red deer (Cervus elaphus) population in Sondrio
Province (Central Italian Alps) were carried out collecting 203 samples of blood and 70 tissue samples from
hunted individuals culled during 1995-2007 period. The seroprevalence was about 6.4% by MAT technique.
The serovariant identified were Australis/Bratislava, Grippotyphosa, Pomona, Sejroe/Hardjo, Copenaghen.
ID nr. 3913
Scordella G, Rubini° S, Fedrizzi ° G, Bresolin R
Seafood safety laws in E.U. markets
Glob Aquac Advocate. - Vol. 2008). - p 26-28
To meet citizens high expectations, European Union seafood regulations implement quality management and
process-oriented controls throughout the food chain. E.U. food businesses must work to reduce risks for
consumer health. Imports must meet E.U. requirements and come from approved establishments inspected
by the competent authority of the exporting country. When problems arise, the Rapid Alert System for Food
and Feed notifies member states and the European Commission.
ID nr. 3914
Pag. 77 di 300 31/08/2011 Cardeti G, Ponticello L, Cittadini M, Lavazza° A, Amaddeo D, Formato G
Practical considerations on bee viral diseases
Apitalia. - Vol. 2 ( 2008). - p 18-20
Project PRC2007110 - Epidemiological study on notifiable bee diseases and evaluation of the relative
sanitary legislation - Formato dr.Giovanni - UO - MINSAN - 2007 - 4950,00 EUR - 01/12/2008
0.00.00 - 30/11/2010 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza
Dr. Antonio - I
Viral diseases of bees need specific diagnostic methods. The severity of infestation by Varroa destructor,
widely diffused and distributed in Italian apiaries, often represents the primary cause of the development of
viral infections which can lead to mortality of the colonies. Therefore, particularly attention should give to bee
viruses, even when present as sub-clincal infection. Nevertheless many data are still unclear and further
studies are needed to better understand pathogenetic patterns of infections such as(route of transmission and
type of damaged induced to bee as well as to define the more useful strategies to control viral infections when
established in a colony.
ID nr. 3915
Vassallo C, Ardigò M, Brazzelli V, Zecca M, Locatelli F, Alessandrino PE, Lazzarino M,
Corona S, Lanzerini P, Benazzo M, Fabbi° M, Borroni G
Bartonella-related pseudomembranous angiomatous papillomatosis of the oral cavity associated
with allogeneic bone marrow transplantation and ora graft-versus-host disease
Br J Dermatol. - Vol. 157 ( 2007). - p 174-178. - 30 bib ref
Impact factor: 3,334
Patients undergoing allogeneic stem cell transplantation are at high risk for infection with a variety of
pathogens during different phases of the procedure. Human infections due to Bartonella spp. are viewed as
emerging diseases typical in, although not exclusive to, immunosuppressed patients, in particular those with
AIDS, organ transplants and haematological malignancies. We describe four patients, three children and one
adult, who developed vegetating papillomatous lesions exclusively on the oral mucosae. They shared a
history of haematological malignancy and allogeneic bone marrow/stem cell transplantation, and
laterdeveloped chronic graft-versus-host disease, also involving the oral mucosae. Histopathologically, the
vegetating lesions were characterized by a diffuse neo angiogenesis, granulation-like tissue, and a mixed cell
infiltrate predominandy composed of neutrophils. Gram-negative bacteria were found in the endothelial cells
of the vessels in the deeper portion of the corium by electron microscopy. In three cases, DNA of B. henselae
was detected by polymerase chain reaction (PCR), and confirmed by sequencing of the PCR products. All the
lesions healed after systemic antibiotic therapy, although some recurred after months, and regressed again
after systemic antibiotic treatment associated with conservative surgical excision.
ID nr. 3919
Marcolini° A, Varisco° G, Bertocchi° L, Bolzoni° G
Estimation of chlorides in bulk milk
Latte. - Vol. 82 no 2 ( 2009). - p 54-58
First author Marcolini, Antonio
Experimental work to verify the possibility to estimate chlorides in large number samples by automated.
ID nr. 3920
Pag. 78 di 300 31/08/2011 Spaggiari° B, Bonilauri° P, Bertocchi° L, Fantini V, Gelmetti° D, Dottori° M
Monitoring of respiratory disease of beef calf at slaughter: a standardized method for lung and
pleural scoring
Summa anim reddito. - Vol. 3 no 9 ( 2008). - p 8-13. - 17 bib ref
First author Spaggiari, Brunella
Beef calf respiratory disease causes direct and indirect losses, due to death or decrease in cattle average
daily gain, respectively. A standardized method is presented for lung and pleural lesions scoring of beef calf at
slaughter
.
ID nr. 3922
Radaelli E, Del_Piero F, Aresu L, Sciarrone F, Vicari° N, Mattiello S, Tagliabue° S, Fabbi°
M, Scanziani E
Expression of major histocompatibility complex class II antigens in porcine leptospiral nephritis
Vet Pathol. - Vol. 46 no 5 ( 2009). - p 800-809 - 37 bib ref
Impact factor: 1,443
Class II major histocompatibility complex (MHCII) is required for the presentation of antigens to CD4 helper T
cells. During nephritis, not only primary antigen presenting cells such as histiocytes and lymphocytes, but also
cytokine-stimulated tubular epithelial cells express MHCII. Leptospirosis in fattening pigs is characterized by
several degrees of nephritis, from absence of lesions to severe multifocal tubulo-interstitial inflammation.
Renal tissue from 20 8-month-old pigs with spontaneous nephritis and 6 control pigs without renal lesions
were investigated for leptospirosis by indirect immunohistochemistry (IHC) and polymerase chain reaction
(PCR). IHC for MHCII also was performed on renal samples. Serum samples were tested for different
serovars of Leptospira interrogans. Control pigs were free of interstitial nephritis and negative for leptospirosis
by all tests. In pigs with nephritis, serology was positive for serovar Pomona in 19/20 pigs. In 16 of these 19
pigs, leptospiral renal infection was confirmed by PCR and/or indirect IHC. Nephritic lesions were classified
histologically into perivascular lymphocytic (4 pigs), lymphofollicular (6 pigs), lymphohistiocytic (8 pigs), and
neutrophilic (2 pigs) pattern. MHCII expression by histiocytes and lymphocytes was observed in all lesions.
Prominent MHCII expression in regenerating tubular epithelium was observed in lymphofollicular and
lymphohistiocytic nephritis. No tubular colocalization between leptospiral and MHCII antigen was observed.
Results suggest that during leptospiral nephritis, MHCII contributes to the intensity of the inflammatory
response. Furthermore de novo MHCII expression in regenerating tubules may play a role in the defence
mechanism against leptospiral tubular colonization.
ID nr. 3923
Pozzi W, Vezzoli° F, Blanchaert A, Falco F
Porcine reproductive and respiratory syndrome and Aujeszky disease virus : intradermal vaccination
with combined or single antigens
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 425-429. - 4 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
In the modem swine industry, vaccination schemes are becoming progressively more intensive, in order to
optimize productive performance in both breeders and fattening animals, but also time-consuming. Concems
remain in respect of the risks associated with parenteral administration such as diseases transmission, tissues
reaction, broken needles and behavioural responses, the last of these being of most importance in breeding
sows and gilts which usually require several doses during the year. Intradermal, needle-less vaccination and
combinations of antigens may help in reducing workloads, disease risks and concerns about animal welfare.
Pag. 79 di 300 31/08/2011 In this trial, safety and immunological efficacy of two antigens, PRRSV and ADV, were analyzed when given
at the same time, either separately, or in combination, via the intradermal route.
ID nr. 3924
Blanchaert A, Vezzoli° F, Pozzi P
PRRS and ADV : results obtained from the intradermal vaccination of gilts using the vaccines
separately or in combination
20th Internationl Pig Veterinary Science Congress IPVS 2008 : Durban, South Africa, 22-26 June 2008 :
poster proceedings / [s.l : s.n, 2008]. - p 413. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20 : Durban, (South Africa) : 22-26 June 2008)
not found.
ID nr. 3925
Donati M, Laroucau K, Storni E, Mazzeo C, Magnino° S, Di_Francesco A, Baldelli R,
Ceglie L, Renzi° M, Cevenini R
Serological response to pgp3 protein in animal and human chlamydial infections
Vet Microbiol. - Vol. 135 ( 2009). - p 181-185. - 19 bib ref
Impact factor: 2,370
Specific antibodies to plasmid-encoded protein pgp3 are known to be encountered in human Chlamydia (C.)
trachomatis infections. In order to verify whether antibodies to this protein could be developed in animals
infected with plasmid-carrying chlamydial strains, 454 animai sera were examined using a home-made pgp3
protein ELISA and Western blots (WB) of recombinant pgp3 protein from Chlamydophila (Cp.) psittaci.
Likewise, 50 human sera were tested by ELISA and WB of recombinant pgp3 from C. trachomatis. The
reactivity against pgp3 protein was compared to the reactivity against chlamydial elementary bodies (EBs)
detected by microimmunofluorescence (MIF) test. The presente of pgp3-specific antibodies was demonstrated
in most ducks and pigeons with Cp. psittaci infection detected by MIF, as well as in the majority of
symptomatic cats and pigs infected with Cp. felis and C. suis, respectively, which reacted at high titres to Cp.
felis and C. suis EBs by MIF. Moreover, most of the sera collected from patients with C. trachomatis cultureconfirmed infection and seropositive to C. trachomatis by MIF, presented antibodies dpecific to C trachomatis
pgp3 recombinant protein. Therefore, pgp3 protein could be a useful marker of chlamydial infections in
animals, as well as in humans.
ID nr. 3926
Revez J, Rossi M, Renzi° M, Zanoni RG
Occurrence of Campylobacter SPP. in italian rabbit farms
Proceedings of the 9th World rabbit congress : Verona (Italy) June 10-13, 2008 / editors Gerolamo Xiccato,
Angela Trocino and Steven D. Lukefahr. - 1. ed. - Brescia : Fondazione Iniziative Zooprofilattiche e
Zootecniche, 2008. - (Atti delle Giornate di Studio Fondazione Iniziative Zooprofilattiche e Zootecniche ; 72)
p 299
World rabbit congress (9 : Verona (Italy) : June 10-13, 2008)
First author Renzi, Maria
In order to investigate the occurrence of Campylobacter spp. in rabbits reared in intensive and rural farms, the
caecal contents of 39 animals from 13 different farms (3 rabbits per farm) were collected from April to
November 2007. The whole intestinal tract from each rabbit was obtained just after evisceration at the
Pag. 80 di 300 31/08/2011 slaughterhouse or during necroscopy, and processed within 4 hours. Approximately 5 g of caecal contents
were squeezed into 5 ml of sterile saline and shaken in order to obtain a homogenous suspension. Samples
were inoculated by streaking 10 µl of each suspension directly onto four different selective fresh media:
Blaser-Wang's Agar (Oxoid), Skirrow's Agar (Oxoid), Nutrient Agar N°2 (Oxoid) 5% sheep blood plus CAT
Selective Supplement (CAT, Oxoid) and modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA,
Oxoid). In addition, samples were inoculated on a non selective medium such as Nutrient Agar N°2 (Oxoid)
5% sheep blood using a modified filter technique of Steele & McDermott. Ali plates were incubated in a jar at
37°C±1 under a microaerobic atmosphere with hydrogen and examined daily for growth up to 12 days. From
each sample, 3 colonies showing the same morphotype referable to Gram negative, curved or spiral rod
bacteria, were cloned. Ali the selected colonies were subjected to genus-specific PCR for Campylobacter.
Positive isolates were submitted to the PCRs specific for C. jejuni, C. coli, C. upsaliensis, C. helveticus and C.
lari. The isolates which resulted negative to the species-specific PCRs were subjected to rpoB sequence
phylogenetic analysis.A total of 36 out of 39 animals (92.3%) and ali the 13 farms resulted positive for
Campylobacter. Ali isolates were positive for Campylobacter genus PCR but negative for ali the speciesspecific PCRs tested. Phylogenetic analysis based on the partial nucleotide rpoB sequences of 13 isolates
(one strain per farm) randomly selected and the reference strains showed that ali the rabbit isolates clustered
together in a tight clade. This cluster was clearly separated from ali the other Campylobacter species with high
bootstrap values (100), indicating that these isolates may belong to a new species. This survey allowed
reporting the occurrence of a probably new Campylobacter species in the caecal contents of farmed rabbits in
Italy. Further studies are necessary to describe it and evaluate its possible pathogenic effect on rabbit as well
as the eventual zoonotic role.
ID nr. 3926a
Revez J, Rossi M, Renzi° M, Zanoni RG
Occurrence of Campylobacter SPP. in italian rabbit farms
http://world-rabbit-science.com/WRSA-Proceedings/Congress-2008-Verona/Papers/P-Revez.pdf (ultimo
accesso 20 febbraio 2009). - Vol. 2008). - p 1051-1054. - 10 ref bib
World rabbit congress (9th : Verona (Italy) : June 10-13, 2008)
In order to investigate the occurrence of Campylobacter spp. in rabbits reared in intensive and rural farms, the
caecal contents of 39 animals from 13 different farms (3 rabbits per farm) were collected from April to
November 2007. The whole intestinal tract from each rabbit was obtained just after evisceration at the
slaughterhouse or during necroscopy, and processed within 4 hours. Approximately 5 g of caecal contents
were squeezed into 5 ml of sterile saline and shaken in order to obtain a homogenous suspension. Samples
were inoculated by streaking 10 µl of each suspension directly onto four different selective fresh media:
Blaser-Wang's Agar (Oxoid), Skirrow's Agar (Oxoid), Nutrient Agar N°2 (Oxoid) 5% sheep blood plus CAT
Selective Supplement (CAT, Oxoid) and modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA,
Oxoid). In addition, samples were inoculated on a non selective medium such as Nutrient Agar N°2 (Oxoid)
5% sheep blood using a modified filter technique of Steele & McDermott. Ali plates were incubated in a jar at
37°C±1 under a microaerobic atmosphere with hydrogen and examined daily for growth up to 12 days. From
each sample, 3 colonies showing the same morphotype referable to Gram negative, curved or spiral rod
bacteria, were cloned. Ali the selected colonies were subjected to genus-specific PCR for Campylobacter.
Positive isolates were submitted to the PCRs specific for C. jejuni, C. coli, C. upsaliensis, C. helveticus and C.
lari. The isolates which resulted negative to the species-specific PCRs were subjected to rpoB sequence
phylogenetic analysis.A total of 36 out of 39 animals (92.3%) and ali the 13 farms resulted positive for
Campylobacter. Ali isolates were positive for Campylobacter genus PCR but negative for ali the speciesspecific PCRs tested. Phylogenetic analysis based on the partial nucleotide rpoB sequences of 13 isolates
(one strain per farm) randomly selected and the reference strains showed that ali the rabbit isolates clustered
together in a tight clade. This cluster was clearly separated from ali the other Campylobacter species with high
bootstrap values (100), indicating that these isolates may belong to a new species. This survey allowed
reporting the occurrence of a probably new Campylobacter species in the caecal contents of farmed rabbits in
Italy. Further studies are necessary to describe it and evaluate its possible pathogenic effect on rabbit as well
as the eventual zoonotic role.
Pag. 81 di 300 31/08/2011 ID nr. 3927
Bellini° S, Alborali° L, Bonazza V, Avisani° D, Zanardi° G
Swine Vesicular Disease in Lombardy Region : pattern of spread in a high density pig area
International Meeting on Emerging Diseases and Suveillance IMED : Vienna, Austria, February 13-16, 2009
: Final Program / [s.l. : s.n., 2009]. - p 96
International Meeting on Emerging Diseases and Suveillance : Vienna, Austria : February 13-16, 2009)
First author Bellini, Silvia
Swine vesicular disease (SVD) is a vesicular condition of pigs induced by an Enterovirus. Although the
disease is frequently mild in nature, il was included in List A of the OIE for the similarity of its lesions to those
produced by Foot-and-Mouth Disease (FMD) Even though compare to FMD. SVD is considered moderately
contagious morbidity is lower and the lesions less severe. In Europe in the last decade. SVD has been
persistently reported in Italy and for this reason surveillance and eradication activities are in place. In the
period 2006-2007 SVD Spread widely in the Italian Northern Regions. Lombardy. a densely populated pig
area, was most affected and difficulties were encountered in eradicating the disease. Even though SVD is
considered to be moderately contagious, the 2006-2007 epidemic in Lombardy was characterized by a rapid
Spread of the condition: 53 outbreaks ,were detected and some 150.000 pigs were stamped out. To verify the
Pattern of disease spread in high-density-pig areas and to highlight risk factors the epidemiological
investigations were carried out in the outbreaks and evaluated. The outbreaks reported in the period may be
grouped in two epidemic periods. During the first one SVD spread among the farms according to the typical
pattern of transmission. In the second period. instead. the diseases showed an endemic trend in a small
portion of the region on (27 km ) where the main risk factor for outbreaks, was proximty to a previous outbreak. To achieve eradication in this area. It was necessary depopulate a group of pig farms, considered at
risk of infection.
ID nr. 3928
Pistone D, Maioli G, Pajoro° M, Fabbi° M, Vicari° N, Epis S, Bandi C, Defilippo° F,
Dottori° M
Rickettsia slovaca in Dermacentor marginatus ticks removed from wild boars (Sus scrofa) in Emilia
Romagna Region, Italy
International Meeting on Emerging Diseases and Suveillance IMED : Vienna, Austria, February 13-16, 2009
: Final Program / [s.l. : s.n., 2009]. - p 141
International Meeting on Emerging Diseases and Suveillance : Vienna, Austria : February 13-16, 2009)
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
Background: Various tick-borne pathogens occur in the Emilia Romagna region (Italy), where Lyme disease,
transmitted by Ixodes ricinus, is frequently diagnosed in humans. The epidemiology of other diseases
vectored by ticks is still to be defined in this area, e.g. the tick-borne lymphadenopathy TIBOLA or DEBONEL
caused by Rickettsia slovaca. Recently, human cases of TIBOLA have been reported in Italy, but in a different
region (Tuscany; Selmi et al. 2008). Abundance of wild boars and ticks of the species Dermacentor
marginatus in the Emilia Romagna region suggests a high risk for the transmission of the R. slovaca. Thev
aim of this study is to determine the prevalence of R. slovaca in D. marginatus ticks removed from hunted wild
boars, in order to evaluate the risk of infection for humans. Methods: Seventynine ticks were removed from 18
hunter-killed wild boars from four different areas of Emilia Romagna region during the period SeptemberNovember 2008. Ticks were identified as D. marginatus using standard taxonomic keys. DNA was extracted
from ticks using a commercial kit and PCR analysis was applied to determine the presence of Rickettsia spp.
Pag. 82 di 300 31/08/2011 Different primers sets were employed, targeted for gltA, gyrB, D-antigen genes. Sequencing of the
amplification products allowed identification of R. slovaca in a subset of the PCR-positive samples. Results: A
total of 50 ticks (50/79-63%) yielded the expected bands by PCR amplification. The sequencing of a subset of
the positive samples confirmed the presence of R. slovaca. Conclusion: Our results shows that R. slovaca is
present in ticks of the species D. marginatus collected on wild boars in Emilia Romagna. Work is in progress
to obtain gene sequencing from all of the positive samples, in order to generate a clearer picture of the
Rickettsiales circulating through D. marginatus in this region. The potential pathogenic role of R. slovaca is
well documented, and the risk for transmission to humans in Emilia Romagna must be considered.
ID nr. 3929
Calzolari° M, Bonilauri° P, Bellini R, Veronesi R, Barbieri° I, Maioli° G, DeFilippo° F,
Dottori° M
Detection of an unknown Flavivirus in Aedes caspius mosquitoes in Valli di Comacchio (Italy)
International Meeting on Emerging Diseases and Suveillance IMED : Vienna, Austria, February 13-16, 2009
: Final Program / [s.l. : s.n., 2009]. - p172
International Meeting on Emerging Diseases and Suveillance : Vienna, Austria : February 13-16, 2009)
First author Calzolari, Mattia
Background: A lot of known flaviviruses are transmitted by arthropods (arbovirus) to men and animals causing
severe disease. Recently, Italy was involved in two important outbreaks of human arbovirus diseases
(Chikungunya and West Nile). In 2007 a regional monitoring program began to detect arboviruses in areas
with high density of mosquitoes. We present here the preliminary results of this program. Methods:
Mosquitoes were collected with CO2 traps in a variety of wetland locations near Comacchio (Ferrara province)
at the end of mosquito season (September-October). Mosquitoes were pooled according to date, location, sex
and species. Pools were tested with a Flavivirus genus PCR targeting gene NS5 (260bp) for the simultaneous
detection of all flavivirus causing important human and animal disease according to Scaramozzino et al.
(2001). Positive Pools were sequenced and analyzed using the ClustalX 2 program. Results: A total of 1267
mosquitoes (81 pools) belonging to the species Aedes caspius, Aedes albopictus and Culex pipiens were
tested and 3pools of Ae. caspius resulted positive for Flavivirus genus. The 3 positive pools were sequenced
and the Blast analysis revealed a certain similarity (less than 80%) with Culex Flavivirus (CuFV) isolated from
Culex pipiens in Japan. Figure 1 shows the Neighbor Joining tree analysis, performed on the same 260 bp
fragment of different flavivirus put the detected virus together with flaviviruses isolated only from insects
(CuFV, cell fusing agent and Kamiti River virus). This group of sequences is clearly separated from
flaviviruses causing diseases in humans and animals Conclusion: The detected flaviviruses probably belong
to a group of virus that are present only in mosquitoes species, so this virus daes not represent a risk for
human and animal populations. At the same time, these positive PCR detections demonstrate that a flavivirus
causing human and animal diseases could be detected, if present, by our regional monitoring program. The
presence of these mosquito flaviviruses must be taken into consideration in any future monitoring programs
conducted in the same area. Finally, future experimental investigation is needed to characterize this virus
.
ID nr. 3930
Lelli° D, Moreno° A, Lavazza° A, Sozzi° E, Luppi° A, Canelli° E, Tamba° M, Capucci° L,
Cordioli° P
Chikungunya : monoclonal antibodies production and their employment in serological diagnosis
International Meeting on Emerging Diseases and Suveillance IMED : Vienna, Austria, February 13-16, 2009
: Final Program / [s.l. : s.n., 2009]. - p 186
International Meeting on Emerging Diseases and Suveillance : Vienna, Austria : February 13-16, 2009)
First author Lelli, Davide
Background: Chikungunya fever epidemic, first evidenced in Italy in 2007, represents the first autochthonous
Pag. 83 di 300 31/08/2011 European outbreak of a tropical disease transmitted by vectors (1,2). Monoclonal antibodies (MAbs) specific
to Chikungunya virus (CHIKV) were produced and used to develop a competitive ELISA test for anti-CHIKV
antibody detection in animal sera from different species collected in the area of the CHIKV outbreak.
Methods: Virus used for MAbs production and as antigen in the ELISA test was strain 209395/07 isolated from
an insect pool (Aedes Albopictus). Screening and characterization of MAbs were performed by indirect ELISA,
immunoperoxidase, virusneutralization (VN) and Western blotting (WB). Twenty known human sera (10
positive and 10 negative) and 493 animal sera (256 dog, 123 pigeon, 79 chicken, 28 nutria and 7 rabbit sera)
were analysed.
Results: Forty five specific MAbs were produced, 9 with VN activity. Two of these (1H7 and 1E10) resulted
positive in WB (3). Two neutralizingMAbs (1H7 and 1A7) were further selected, cloned and conjugate with
HRP for the development of a competitive ELISA test. MAb 1H7 reacted against a linear epitope while 1A7
against a conformational epitope located both within the E2 protein. The ELISA test was developed using in
parallel the 2 conjugated MAbs. Nunc 96 wells plates were coated with partially purified antigen, four dilutions
(from 1/5 to 1/40) of each sera were distributed, followed soon afterwards by the addition of selected MAbconjugates. The ability of sample sera to inhibit the binding of specific MAbconjugate to the antigen was then
evaluated and results were expressed as percentage of inhibition. The human sera were correctly identified
whereas all the 493 sera resulted negative.
Conclusion: The serological diagnosis represents a valid diagnostic tool in the study of the epidemiology of
this disease and the effective role of animals in the virus spreading.
ID nr. 3934
Tittarelli° C, Cerioli° M, Canelli E, Lavazza° A
Diagnosis of enteric viral diseases of dogs in Northern Italy during the period 2002-2008, by using
negative staining immuno-electron-microscopy (IEM)
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
300-301. - 10 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10 : Alghero : 2224 Ottobre 2008)
First author Tittarelli, Cristiana
The Immuno-electronmicroscopy (IEM) is a sensitive and specific method to detect enteric viruses. The aim of
this study is to report the data of identification by IEM of viral agents in diarrheic dogs during the period 20022008 in Northern Italy. The presence of virai particles was observed in 32.2% out of 873 total samples
examined by IEM. Parvovirus was identified in 28.9% of them, morbillivirus in 1,8%, coronavirus in 0,9%,
calicivirus in 0,6%, enterovirus and herpesvirus in 0,3%, rotavirus, reovirus e paramyxovirus-like in 0,1%. In
13 cases 2 different viruses were observed in association in the same sample.
ID nr. 3935
Tittarelli° C, Tranquillo° V, Luppi° A., Nassuato° C, Grilli GJ, Lavazza° A
Serological survey for Encephalitozoon cuniculi in pet rabbits: preliminary data
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
298-299. - 8 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10 : Alghero : 2224 Ottobre 2008)
First author Tittarelli, Cristiana
During a 10 months-period, 325 sera have been collected from 304 pet rabbits and submitted through Carbon
Immuno Assay (CIA) test, to an antibody research for Encephalitozoon cuniculi. Seropositivity was observed
in 56.2 % out of 304 examined animals. Seroprevalence in the symptomatic rabbits was 28.8%. This
Pag. 84 di 300 31/08/2011 preliminary study supplies originai information about epidemiology of this microsporidium, which is largely
wide-spread in pet rabbits living in Italy.
ID nr. 3936
Rugna° G, Bardasi° L, Vecchi° G, Mazzini C_Z, Bacchi M, Galletti° G, Merialdi° G,
Fontana° MC
Post-packaging pasteurization treatment on mortadella experimentally contamined with Listeria spp.
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
284-285. - 9 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
First author Rugna, Gianluca
The presence of Listeria monocytogenes in ready-to-eat (RTE) meat products is cause of public-health
concern. To reduce the risk of food borne infection and ensure food safety, RTE meat cooked-products can
be pasteurized after packaging. In this study the efficacy in reducing L. innocua superficial contamination of
three in-package pasteurization protocols (A: 89 "C x 6 min; B: 89 "C x 8 min; C: 89 "C x 10 min) was
evaluated. Treatment C ensured superficial contamination reduction also in particularly irregular surfaces like
those at the edges of product and can be an effective intervention to reduce L. monocytogenes contamination
risk in such products.
ID nr. 3937
Pezzoni° G, Brocchi° E
A baculovirus expressed NS3 protein of bovine viral diarrhea virus displays conformational and
antigenical properties as the native protein
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
264-265. - 2 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10 : Alghero : 2224 Ottobre 2008)
Project PRC2005012 - Production and characterization of recombinant viral proteins as antigens for
serology - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2005 - 68000,00 EUR - 04/09/2006 0.00.00
- 03/09/2009 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Pacciarini Dr.ssa Maria - I
First author Pezzoni, Giulia
One of the major immunological proteins of Pestiviruses is the nonstructural protein 3 (NS3), a multifunctional
enzyme with at least two domains associated with enzymatic activities: a serine protease activity and a
NTPase-elicase activity. This study describes the expression of the entire sequence of NS3 (NS3tot) and its
NTPase-elicase domain (NS3E) in a baculovirus system. Both recombinant proteins are recognised by
monoclonal antibodies specific for three different conformational epitopes, as well as by BVDV-positive sera.
These reactivities provide evidence that the recombinant proteins obtained reproduce folding and antigenicity
of the native virai proteins and provide the conditions for the development of a functional antibodydetection
ELISA.
ID nr. 3939
Martinelli° N, Luppi° A, Lelli° D, Sozzi° E, Canelli° E, Fontana R, Moreno_Martin° A,
Lavazza° A, Lombardi° G.
Pag. 85 di 300 31/08/2011 Investigation on the presence of pathogens in laboratory mice and rats of breeding and testing
centers
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
238-239. - 9 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10 : Alghero : 2224 Ottobre 2008)
First author Martinelli, Nicola
A survey to detect antibodies against hepatitis E virus (HEV) was undertaken on 39 Italian pig farms (17
farrow to feeder, 10 farrow to finish and 12 fattening herds). For the study 1422 pig sera samples were tested
using a commerciai indirect ELISA, originally developed for testing human sera and properly adapted for the
analysis of pig sera. 38 of the farms (97,43%) and 714 on 1422 sera samples (50,21%) resulted positive for
anti-HEV IgG antibodies. The study confirm that HEV is spread in pigs in Italy and is probably endemic in
many farms.
ID nr. 3941
Cardeti G, Lavazza° A, Cittadini M, Ponticello L, Formato G, Tittarelli° C, Amaddeo D
Bee viruses and mortality of colonies
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
136-137. - 9 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
Specific viruses affecting honeybees are widespread and normally give latency i.e. they can be detected in
seemingly healthy bees. In the fast decade we investigated the presence and spread of bee viruses in Italian
hives. Samples of honeybees at different stages were examined by electron microscopy methods, including
IEM for ABPV and ELISA test for DWV. DWV and ABPV were often detected in high titres in association with
severe signs: depopulation, adult bees with paralysis and/or deformed wings, mortality.
ID nr. 3942
Canelli° E, Luppi° A, Barbieri° I, Lavazza° A, Moreno_Martin° A, Sozzi° E, Lelli° D,
Cordioli° P
Genetic typing of BVDV strains isolated in Lombardia and Emilia Romagna regions from 1999 to
2007
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
128-129. - 10 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
Project PRC2008016 - An investigation into prevalence of Pestivirus infection in cattle and swine herds. Sozzi Dr.ssa Enrica - Capofila - MINSAN - 2008 - 70000,00 EUR - 01/09/2009 0.00.00 31/08/2011 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Sozzi Dr.ssa
Enrica - I
2 - IZSUM - De Mia Dr. Gian Mario - E
First author Canelli, Elena
A panel of 115 BVDVs isolated in the 1999-2007 period from cattle breed in Lombardia and Emilia Romagna
Pag. 86 di 300 31/08/2011 regions was subtyped by sequencing and phylogenetically analyzed. Primers used target a fragment of the
5'UTR region of BVDV genome. The data obtained show that the isolates belong lo 9 subtypes within BVDV1, indicating a high level of genetic heterogeneity. Two subtypes never reported before in Italy are described.
BVDV-1b and 1e are the most frequent isolated subtypes. The low frequency of BVDV-2 isolates is in
agreement with the sporadic Italian occurrence.
ID nr. 3943
Cammi° G, Arrigoni° N, Belletti° GL, Garilli F, Ricchi° M, Vicari° N, Tamba° M, Galletti° G
Prototheca in bovine bulk tank milk in Northern Italy
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
120-121. - 9 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
First author Cammi, Giuliana
Bulk tank milk samples collected from 350 dairy herds located in the Po Valley (Northern Italy) were
investigated for Prototheca spp. Ali samples were cultured on PIM at 30 "C for 72 h for total number of
Prototheca cells and analyzed also for total bacteria count and somatic cells count. Prototheca spp. were
isolated in milk sample from 54 (15,43%) dairy herds. No statistical correlation resulted between Prototheca
spp. isolatìon and bulk tank milk somatic celi counts and total bacterial count.
ID nr. 3948
Pezzoni° G, Brocchi° E
Recombinant NS3 and monoclonal antibodies for serodiagnosis of pestiviruses infection by
competitive ELISA
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
80-81. - 2 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
Project PRC2005012 - Production and characterization of recombinant viral proteins as antigens for
serology - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2005 - 68000,00 EUR - 04/09/2006 0.00.00
- 03/09/2009 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Pacciarini Dr.ssa Maria - I
First author Pezzoni, Giulia
Bovine Viral Diarrhea Virus (BVDV) belongs to Pestivirus group with viruses responsible for Border Disease
and Classical Swine Fever; the three viruses are antigenically related. Currently, serologicai assays for
Pestiviruses are based on evaluation of antibody against the non structural protein 3 (NS3). This study reports
on the use of a baculovirus expressed NS3 in substitution of Pestivirus as source of antigen in two ELISA
formats (trapping and competitive) for detection of antibodies in bovine and swine sera. The discrimination
power between positive and negative sera provided by assays using the recombinant antigen led to a
sensitivity and specificity of 100% in relation to analogous ELISAs based on virus.
ID nr. 3949
Scicluna MT, Zini M, Caprioli A, Cordioli° P, Vulcano G, Della_Verità F, Gregnanini S,
Palmerini T, Simula M, Stilli D, Autorino GL
Pag. 87 di 300 31/08/2011 Equine infectious anemia : should the agar immunodiffusion test still be used for screening and as
unique confirmatory test ?
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
78-79. - 7 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
Following the introduction in 2007, of an extraordinary surveillance programme, imposing the serological
control of the Italian equine population for infectious anemia (EIA), our laboratory adopted as screening test, a
previously validated in-house p26 CElisa. Samples with positive or doubtful results, were subsequently
confirmed by the agar ímmunodiffusion (AGID), the prescribed confirmatory test. The Western blot (WB)
assay, recommended by World Organisation Animai Health (WOAH) as compiementary test, was applied for
the first time in the EIA contro) programme, for routine samples with equivocai results occurring in our
laboratory (positive/doubtful CElisa-negative AGID). Over a 17-month period. 253 sera resulted reactive out of
the 32 448 horses bled in the province of Rome. Among the former, 83 were confirmed positive in AGID. For
those available, i.e. 91 sampies, further analysis in WB was carried out. Ten of the CElisa pos/AGIDneg
horses were confirmed positive, because reactive with both core protein p26 band and al least one of the 2
surface glycolproteins bands, gp45 and gp90, considered this as specific response pattern to an EIA infection.
These preliminary results demonstrate the greater sensitivity of the in-house CElisa compared to AGID,
important characteristic in a screening test. For such equivocai results, the in series use of Elisa and AGID
and/or WB, improves the overail specificity of EIA diagnosis, essential in the eradication of this low prevalent
infection.
ID nr. 3950
Sozzi° E, Barbieri° I, Lavazza° A, Moreno° A, Lelli° D, Luppi° A, Canelli° E, Bugnetti° M,
Cordioli° P
Genetic characterization of Porcine enterovirus e testovirus isolated in taly in 2006-2007
X Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) : 22-24 Ottobre
2008 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2008]. - p
66-67. - 7 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (10. : Alghero : 2224 Ottobre 2008)
First author Sozzi, Enrica
Porcine enterovirus (PEV) and Teschovirus (PTV), belonging to the family Picornaviridae, are ubiquitous and
mainly cause asymptomatic infections in pigs. In the present study a total of 40 Italian porcine enteroteschovirus isolates were characterized by the analysis of the sequences of the capsid VP1 encoding gene.
The assay turned out to be a useful diagnostic tool for the molecular diagnosis of porcine
teschovirus/enterovirus strains and for the study of molecular epidemiology and evolution of these viruses
confirming the possibility of correlating virus genotype to serotype.
ID nr. 3952
Candotti° P, Rota_Nodari° S, Berti F, Ostanello F, Meliota F
Efficacy of a new vaccine (Myco-Suivax®) against Mycoplasma hyopneumoniae under field
conditions
20th IPVS Internationl Pig Veterinary Society Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - V. 2. - cdrom p 195. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, South Africa : 22-26 June 2008)
First author Candotti, Paolo
Pag. 88 di 300 31/08/2011 not found.
ID nr. 3956
Grazioli° S, Brocchi° E, Tranquillo° V, Parida° S, Paton° D
Development of solid phase competitive ELISAs based on monoclonal antibodies for the serology of
FMDV serotypes SAT1 and SAT2
The global control of IMD tools, ideas and ideals : report European Commission for the Control of Foot-and
Mouth Disease Session of the Research Group of the Standing Technical Committee : 14-17 October 2008
Erice, Italy / [s.l. : Food and Agriculture Organization of the United Nations, 2008]. - p 48
Session of the Research Group of the Standing Technical Committee of the European Commission for the
Control of Foot-and-Mouth Disease : Erice, Italy : 14-17 October 2008)
Project 2003-SSP-3-513645 - New and emerging technologies: improved laboratory and on-site detection of
oie list a viruses in animals and animal products (LAB-ON.SITE) - Belak Dr. Sandor - UO - CE 2004 - 1500000,00 EUR - 01/11/2004 0.00.00 - 31/07/2008 0.00.00 - 7 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
PRC2003015 - Development of reagents (monoclonal and polyclonal antibodies) and diagnostic
assays for detection of SAT type FMD virus. - Grazioli Dr.ssa Santina - Capofila - MINSAN - 2003
- 26/04/2004 0.00.00 - 25/04/2007 0.00.00 - 25000,00 EUR 1 - H1 - Reparto di Biotecnologie Grazioli Dr.ssa Santina - I
2 - FMD World Reference Laboratory (WRL), Institute for Animal Health, Pirbright Laboratory, UK Paton Dr. David - E
First author Grazioli, Santina
Introduction: In the spectrum of Foot-and-Mouth Disease (FMD) diagnostic tools there is a gap in the
availability of standardised and validated immunoassays for the serology of SAT serotypes. Because of the
endemic presence of SAT strains in Africa and the risks due to market globalization, filling this gap should be
a priority. Panels of characterised monoclonal antibodies (MAbs) specific for the serotypes SAT 1 and SAT 2
are available in our laboratory and there is much evidence to indicate that diagnostic ELISAs may benefit from
the use of MAbs in terms of standardisation and
specificity. Within the programme to complete and improve the sets of reagents and diagnostics for FMD, we
have developed Solid Phase Competitive ELISAs (SPCE) for the assessment of antibodies to FMDV types
SAT 1 and SAT 2. In these assays, specific antibodies in serum are detected due to their ability to compete
with a peroxidase-labelled neutralising MAb. Materials and methods: SPCEs were designed with selected
MAbs as catching and labelled-competitor antibody. The latter are neutralising MAbs specific for FMDV SAT 1
(4C5) and SAT 2 (2H6) respectively and directed against linear sites of VP1. More than 1000 known negative
and few positive experimental sera were used for calibration of the assays; then diagnostic performances
were evaluated using cattle sera from different field cases and by comparison with other immunoassays.
Results: Specificity of 100% was found for the SAT 1 antibody-detection ELISA; form the SAT 2 test specificity
improved from 97.5 to 99.6% according to inclusion or exclusion of a doubtful range. A Bayesian analysis on
results obtained with field samples is in progress; preliminary comparative estimates showed for both assays
good correlation with virus neutralisation tests and polyclonal-SPCEs. Satisfactory type-specificity was also
demonstrated by examining sera positive against other FMDV serotypes. Conclusions: The MAb-based SPCE
developed for the detection of antibodies to FMDV types SAT 1 and 2 proved to be a highly specific and
sensitive assays. With respect to SPCEs carried out using rabbit and guinea-pig antisera, the MAb-based
ELISA is simpler, faster and, above all, more standardised, thanks to the employment of unique invariable
MAbs.
ID nr. 3957
Brocchi° E, Crosatti° ML, Grazioli° S, Bugnetti° M
Preliminary evaluation of panels of monoclonal antibodies developed against four vaccine strains of
Pag. 89 di 300 31/08/2011 FMD viruses of serotype A for antigenic profiling and vaccine matching of field isolates
The global control of IMD tools, ideas and ideals : report : European Commission for the Control of Foot-and
Mouth Disease, Session of the Research Group of the Standing Technical Committee : 14-17 October 2008
Erice, Italy / [s.l. : Food and Agriculture Organization of the United Nations, 2008]. - p 85
Session of the Research Group of the Standing Technical Committee of the European Commission for the
Control of Foot-and-Mouth Disease : Erice, Italy : 14-17 October 2008)
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
PRC98004 - Update of reagents and procedures for diagnosis and control of Foot-and-mouth
disease toward recognition of newly arrived viral strains - Brocchi Dr.ssa Emiliana - Solo - MINSAN
- 1998 - 01/10/1999 0.00.00 - 30/09/2001 0.00.00 - 190000000,00 LIT 1 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
First author Brocchi, Emiliana
Monoclonal Antibodies (MAbs) are strategic resources to study the antigenic profile of new field isolates of
Foot-and-mouth Disease (FMD) virus and their antigenic relationships with reference strains. This knowledge
is important to properly select most suited vaccine strains when vaccination is adopted as a control measure
against FMD. Objective of this work was upgrading of MAbs to FMDV type A through the production of new
panels of MAbs to selected vaccine and field strains of type A. Materials and methods: Five strains of type A
were selected as target for the production of MAbs, namely the vaccine strains A 22 Iraq, A24 Cruzeiro, A 15
Tahiland 16/60, A Iran 96 and the field isolate A Malaysia 16/97. A preliminary characterisation of MAbs was
achieved, based on reactivity in ELISA, Virus neutralisation ability, identification of conformational or linear
epitopes and intratype cross reactivity with a panel of 25 strains of type A, including vaccine strains and
representative field isolates. Results: Wide panels of MAbs were obtained against each of the five strains of
FMDV type A. The variety of MAbs obtained, their various profiles of intra-type reactivity that confirmed the
high level of antigenic variability within type A, the diverse reactivity in Western Blot and Virus Neutralising
tests were indicative that these MAbs actually cover different antigenic sites and may be useful for the
creation of panels suitable for antigenic profiling. Discussion: Cross-protection between vaccine and field
strains should mainly rely on neutralising sites; then, antigenic profiling obtained with neutralising MAbs is
more likely to predict the level of cross-protection. In order to complete the characterization of the new MAbs
and verify whether they sufficiently cover the main antigenic sites, mapping of epitopes target of the
neutralising MAbs is in progress.
ID nr. 3958
Brocchi° E, Grazioli° S, Pezzoni° G
Evaluation of profiling ELISA for antibodies to non structural proteins of foot-and-mouth disease
virus for DIVA-serology
The global control of IMD tools, ideas and ideals : report : European Commission for the Control of Foot-and
Mouth Disease, Session of the Research Group of the Standing Technical Committee : 14-17 October 2008
Erice, Italy / [s.l. : Food and Agriculture Organization of the United Nations, 2008]. - p 84
Session of the Research Group of the Standing Technical Committee of the European Commission for the
Control of Foot-and-Mouth Disease : Erice, Italy : 14-17 October 2008)
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
PRC99004 - Development and validation of innovating serological methods for the differentiation of
vaccinated from FMD-virus infected animals based on the use of multiple non-structural antigens Brocchi Dr.ssa Emiliana - Solo - MINSAN - 1999 - 16/08/2000 0.00.00 - 15/08/2002 0.00.00 -
Pag. 90 di 300 31/08/2011 142524000,00 LIT 1 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
First author Brocchi, Emiliana
Introduction: The vaccinate-to-live policy for Foot-and-mouth Disease (FMD) is based on the availability of
validated screening assays to differentiate vaccinated from infected animals based on the detection of
antibodies to FMDV non structural proteins (NSP). The need of confirmatory tests for NSP-serology has
encouraged this study for the determination of serum profiles against multiple NSP. Materials and methods:
Indirect ELISAs for the measurement of antibodies to the NSP 3A, 3B and 3D of FMDV were developed; for
3A and 3D a specific monoclonal antibody was used to trap the respective recombinant antigen. Diagnostic
performances were studied using known sera derived from experimental cattle with a know FMD status. Data
were analysed in comparison with results of a validated 3ABC-trapping ELISA. Results: Early seroconversions
against 3A, 3B and 3ABC were similar, while antibodies to 3D occurred two days earlier; however, antibodies
to 3B were low or not detectable in few animals. Duration of antibodies was evaluated up to 400 days after
infection: results showed that antibodies detected by the 3ABC-ELISA were the most persistent, followed by
antibodies detected by 3A-ELISA, while antibodies detected by 3B- and 3D-ELISA decayed more rapidly.
Specificity for 3A-ELISA was 98.3%, sensitivity reached 100% in infected cattle and 83% in cattle that were
vaccinated prior to infection. These sensitivity estimates are similar to those found for the validated 3ABCtrapping ELISA, consistently with the good correlation observed between the two tests. The specificity for 3BELISA reached 99%, whilst sensitivity was significantly lower in both categories of infected (75%) and
vaccinated/infected (72%) cattle, possibly due to either “3B-nonresponder” cattle or to a faster decay of
antibodies to 3B. Further research is needed to improve sensitivity and specificity of the 3D-trapping ELISA.
Conclusions: 3A and 3B-ELISA may be considered as prototype NSP-ELISAs useful for NSP-Ab profiling.
They do not reach individually the diagnostic performance of the 3ABC-ELISA; however, combined in a
“profiling test” comprising multiple antigens, they may have interesting applications, including a potential use
as confirmatory system.
ID nr. 3959
Grazioli° S, Brocchi° E
Mapping of linear antigenic sites of the non structural protein 3D of foot-and-mouth disease relevant
for the recognition of an infection status
The global control of IMD tools, ideas and ideals : Report. European Commission for the Control of Foot-and
Mouth Disease Session of the Research Group of the Standing Technical Committee : 14-17 October 2008
Erice, Italy / [s.l. : Food and Agriculture Organization of the United Nations, 2008]. - p 91
Session of the Research Group of the Standing Technical Committee of the European Commission for the
Control of Foot-and-Mouth Disease : Erice, Italy : 14-17 October 2008)
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
PRC99004 - Development and validation of innovating serological methods for the differentiation of
vaccinated from FMD-virus infected animals based on the use of multiple non-structural antigens Brocchi Dr.ssa Emiliana - Solo - MINSAN - 1999 - 16/08/2000 0.00.00 - 15/08/2002 0.00.00 142524000,00 LIT 1 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
PRF2006302 - Development of innovative technologies for the diagnosis of infectious diseases of
animals - Patta Cristina - UO - MINSAN - 2006 - 01/02/2008 0.00.00 - 30/06/2011 0.00.00 1664000,00 EUR - 1 - H2 - Reparto di Biologia Molecolare - Brocchi Dr.ssa Emiliana - I
First author Grazioli, Santina
Introduction: The identification of FMDV-infected animals, regardless their vaccination status, is based on the
detection of antibodies to non structural proteins (NSP) of the virus, which are produced only after active viral
replication. In addition to the polypeptides 3ABC (with its derivative products), 3D is a highly immunogenic
NSP and ELISA assays using these antigens as recombinant proteins showed adequate fitness for purpose.
An improved knowledge of the antigenic structure of NSPs and of the immune response of infected animals
Pag. 91 di 300 31/08/2011 against NSPs could generate new perspectives for the differential diagnosis between infection and
vaccination. This study describes the linear antigenic map of 3D, detected by pepscanning analyses of
convalescent sera and monoclonal antibodies. Materials and methods: Peptide scanning analyses were
performed using 20-mer synthetic peptides (48 peptides) overlapping in 10 amino acids (aa), matching the
sequence of 3D. Twenty-nine sera from FMD-convalescent animals (cattle and pigs) and 49 monoclonal
antibodies (MAbs) raised against 3D were tested with each peptide in an indirect ELISA. Results: The immune
response against 3D is restricted towards the N-terminal third of the protein. Regions recognised by
convalescent sera mostly overlap regions target of anti-3D MAbs. Two regions, one spanning from a position
8 to 38 and another shorter one, around position 58, are recognised by the majority of FMD-convalescent
animals and are also target sites for a few MAbs. The sequence flanking residue 168 is a further immunogenic
region for FMDsusceptible species but is not recognised by any MAb, whilst is the region spanning from
residue 128 to 143 reactive with the majority of MAbs. Conclusions: Immunodominant regions have been
identified in the 3D sequence, recognized by both FMD post-infection sera and anti-3D MAbs, corresponding
to discrete intermitted sequences, all comprised within the N-terminal third of the polypeptide. MAbs specific
for these epitopes represent suited tools to develop competitive assaysfor the measurement of antibody to
3D, while synthetic peptides matching the immunodominant regions might substitute the whole protein in
diagnostic assays.
ID nr. 3960
Martín-Acebes MA, González-Magaldi M, Rosas MF, Borrego B, Brocchi° E, ArmasPortela R, Sobrino F
Subcellular distribution of swine vesicular disease virus proteins and alterations induced in infected
cells: a comparative study with foot-and-mouth disease virus and vesicular stomatitis virus
Virology. - Vol. 374 ( 2008). - p 432-433. - 79 bib ref
Impact factor: 3,765
Project PRC2002007 - Improvement of SVD diagnosis: identification and neutralization of swine
enteroviruses affecting SVD virus isolation in tissue cultures. - Brocchi Dr.ssa Emiliana - Capofila MINSAN - 2002 - 208749,00 EUR - 15/04/2004 0.00.00 - 31/12/2006 0.00.00 - 1 - H1 - Reparto
di Biotecnologie - Brocchi Dr.ssa Emiliana - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
3 - IZSVE - Nardelli Dr. Stefano - E
4 - ISS - Tollis Dr.ssa Maria - E
The intracellular distribution of swine vesicular disease virus (SVDV) proteins and the induced reorganization
of endomembranes in IBRS-2 cells were analyzed. Fluorescence to new SVDV capsids appeared first upon
infection, concentrated in perinuclear circular structures and colocalized to dsRNA. As in foot-and-mouth
disease virus (FMDV)-infected cells, a vesicular pattern was predominantly found in later stages of SVDV
capsid morphogenesis that colocalized with those of non-structural proteins 2C, 2BC and 3A. These results
suggest that assembly of capsid proteins is associated to the replication complex. Confocal microscopy
showed a decreased fluorescence to ER markers (calreticulin and protein disulfide isomerase), and
disorganization of cis-Golgi gp74 and trans-Golgi caveolin-1 markers in SVDV- and FMDV-, but not in
vesicular stomatitis virus (VSV)-infected cells. Electron microscopy of SVDV-infected cells at an early stage of
infection revealed fragmented ER cisternae with expanded lumen and accumulation of large Golgi vesicles,
suggesting alterations of vesicle traffic through Golgi compartments. At this early stage, FMDV induced
different patterns of ER fragmentation and Golgi alterations. At later stages of SVDV cytopathology, cells
showed a completely vacuolated cytoplasm containing vesicles of different sizes. Cell treatment with brefeldin
A, which disrupts the Golgi complex, reduced SVDV (approximately 5 log) and VSV (approximately 4 log)
titers, but did not affect FMDV growth. Thus, three viruses, which share target tissues and clinical signs in
natural hosts, induce different intracellular effects in cultured cells.
ID nr. 3961
Canelli° E, Lavazza° A, Barbieri° I, Moreno° AM, Sozzi° E, Lelli° D, Cordioli° P
Pag. 92 di 300 31/08/2011 Prevalence of subtypes 1.1 and 1.2 within BHV1 strains isolated since 2002 in Northern Italy:
antigenic characterization and genetic analysis
3th ESVV Veterinary Herpesvirus Symposium, April 22-24, 2009 Greifswald - Insel Riems / [s.n. : s.l., 2009].
ESVV Veterinary Herpesvirus Symposium (3th : Greifswald - Insel Riems : April 22-24, 2009)
First author Canelli, Elena
Bovine herpes virus type 1 (BHV-1) is wídespread and it causes respiratory and reproductive diseases? in
cattle, but also other less frequent clinical signs. BHV-1 can be classified into two main subtypes: 1.1 is
responsible for the respiratory disease and 1.2 that is mainly associated with genital infections and further
divided into 1.2 a and b. During a seven years period, BHV-1 strains were mostly isolated from respiratory
samples, out of the overall number of samples obtained from either genital or respiratory tracts of bovines
from Northern Italy herds submitted to routine laboratory examination. Thereafter, 45 BHV-1 isolates were
antigenically and genetically characterized and the prevalence of the two subtypes in the analyzed area was
evatuated. BHV-1 strains isolated were typed by using an immunoperoxidase reaction (IP) based on two
different panels of monoclonal antibodies (MAbs), previously developed and identified as specific only for the
BHV-1 type. The MAbs panel 1 (1C11; 4G8) is specific for the sole BHV-1.1 subtype, while the other panel
(1D6; 2610; 2133) detects both subtypes. The IP showed that al[ analyzed strains were BHV-1.1. Genomic
characterization of the strains was done by sequencing and phylogenetic analysis. The amplification reaction
was made with a pair of primers targeting the gl encoding gene. PCR products were sequenced and the
obtained sequences were analyzed and compared with others from representative reference and field strains.
Consequently, the Northern Italian isolates were grouped with the Colorado reference strain of BHV-1.1 and
showed high percentages of homology (higher than 99.4%). The genetic data were in total agreement with the
IP results and underlined that the BHV-1.1 subtype was prevalent in the analyzed area. These findings
correlated well with the results of previous studies conducted in the same area on strains isolated in the 8090s and in other countries (Ireland and UK), but they differed from those obtained in Australia and New
Zealand. Moreover the genetic analysis remarked that the gl showed a high degree of DNA homology among
BHV-1 strains and that there were no genomic variations in the gl region during the years. In conclusion, the
obtained data could help in finding out more correct targets for the improvement of BHV-1 diagnosis and for
the development of more specific vaccines that can be efficiently applied in controt programs.
ID nr. 3962
Rodriguez-Sanchez B, Sanchez-Vizcaino JM, Uttenthal A, Rasmussen TB, Hakhverdyan
M, King DP, Ferris NP, Ebert K, Reid SM, Kiss I, Brocchi° E, Cordioli° P, Hjerner B,
McMenamy M, McKillen J, Ahmed JS, Belak S
Improved diagnosis for nine viral diseases considered as notifiable by the World Organization for
Animal Health
Transboundary Emerg Dis. - Vol. 55 ( 2008). - p 215-225. - 38 bib ref
Impact factor: 0,702
Project 2003-SSP-3-513645 - New and emerging technologies: improved laboratory and on-site detection of
oie list a viruses in animals and animal products (LAB-ON.SITE) - Belak Dr. Sandor - UO - CE 2004 - 1500000,00 EUR - 01/11/2004 0.00.00 - 31/07/2008 0.00.00 - 7 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
Nine viral diseases included in the World Organization for Animal Health list of notifiable diseases (former list
A) were chosen for their contagiousness and high capacity of spreading to improve their diagnosis using new
and emerging technologies. All the selected diseases -- foot-and-mouth disease, swine vesicular disease,
vesicular stomatitis, classical swine fever, African swine fever, bluetongue, African horse sickness, Newcastle
disease and highly pathogenic avian influenza -- are considered as transboundary diseases, which detection
causes the prohibition of livestock exportation, and, thus, it leads to high economical losses. The applied
diagnostic techniques can fall into two categories: (i) nucleic-acid detection, including padlock probes, realtime PCR with TaqMan, minor groove binding probes and fluorescence energy transfer reaction probes,
isothermal amplification like the Cleavase/Invader assay or the loop-mediated amplification technology and
the development of rapid kits for 'mobile' PCR and (ii) antigen-antibody detection systems like simplified and
Pag. 93 di 300 31/08/2011 more sensitive ELISA tests. Besides, internal controls have been improved for nucleic acid-detecting methods
by using an RNA plant virus--Cowpea Mosaic Virus--to ensure the stability of the RNA used as a positive
control in diagnostic real-time RT-PCR assays. The development of these diagnosis techniques has required
the joint efforts of a European consortium in which nine diagnostic laboratories and an SME who have
collaborated since 2004 within the European Union-funded Lab-on-site project. The results obtained are
shown in this paper.
ID nr. 3963
Bano L, Anniballi F, Delibato E, De_Medici D, Agnoletti F, Cocchi M, Drigo I, Magistrali C,
Fontana° MC, Merialdi° G, Arossa C, Fenicia L
Avian botulism in Italy: application of a duplex PCR assay as a useful tool for the isolation of
neurotoxigenic strains
Congress "Clostridium botulinum : epidemiology, diagnosis, genetics, control and prevention" : 16-19 June
2008 / [s.l : s.n, 2008]. - p 66
Congress "Clostridium botulinum : epidemiology, diagnosis, genetics, control and prevention" : Helsinki,
Filand : 16-19 June 2008)
Avian botulism is a paralytic and often fatal disease of wild and farming birds occurring worldwide and caused
by Clostridium botulinum type C and D. The disease is often diagnosed only on clinical and epidemiological
features because of the difficulty of laboratory confirmation. Particularly, the need to isolate the toxigenic
strains in order to produce an auto-vaccine, is a hard step depending on the frequent loose of the gene
encoding the neurotoxin. Avian botulism has been documented also in Italy but, due to the lack of a specific
surveillance system on animal botulism, the epidemiological and clinical data are not already systematically
collected. We reported a recent experience on repeated outbreaks of avian botulism occurred in the North–
East of Italy. A duplex PCR for bont/C and bont/D genes, together with Internal Amplification Control (IAC),
was optimised and successfully utilized for the laboratory confirmation of the outbreak and to facilitate the
isolation of the neurotoxigenic strain used to produce the autovaccine. The IAC was included in the PCR
protocol to avoid false negative results due to the lack of the reaction caused by inhibitory substances. It is
also discussed a project to organize in Italy a surveillance system, in collaboration with the Italian Ministry of
Health, the National Reference Center for Botulism at the ISS, state veterinary diagnostic laboratories (IZS)
and other stakeholders to collect all epidemiological, clinical and microbiological data connected with animal
botulism.
ID nr. 3964
Amadori° M
Physiological response and constitutive expression of interferons : roles and functions
New research on innate immunity / Mathis Durand and Clara V. Morel, editors. - New York : Nova Science
Publischers, Inc, c2008. - p 1-11. - 58 bib ref
First author Amadori, Massimo
Constitutive expression of type I interferon (IFN) has been convincingly demonstrated in lymphoid and nonlymphoid tissues. This finding is a major challenge to the traditional view depicting IFNs as fundamental
antiviral cytokines with other accessory properties. On the contrary, there is strong evidence of a physiological
IFN response, probably linked to a default anti-inflammatory control action in tissues. This is diverted by
microbial infections to pro-inflammatory effector functions in the framework of the innate immune response.
Thus, constitutive expression of type I IFNs should be set into an alternative conceptual framework,
recognizing these cytokines as homeostatic agents with a steady-state role under health conditions. The coexistence in peripheral blood mononuclear ceils of pigs of type 1 and type II (y) IFN outlines a novel scenario,
probably related to the host's need for a fine tuning of the inflammatory and/or immune response to
substantial changes of the external milieau.
Pag. 94 di 300 31/08/2011 ID nr. 3965
Canelli° E, Luppi° A, Fallacara° C, Sozzi° E, Lelli° D, Moreno_Martin° M, Cordioli° P
Genetic characterization of bovine viral diarrhoea viruses isolated from cattle in Northern Italy during
the 1999-2007 period
7th ESVV pestivirus symposium, Uppsala Sweden 16-19 September 2008 / [s.n. : s.l., 2008]. - p 179. - 5 bib
ref
ESVV pestivirus symposium (7th : Uppsala, Sweden : September 16-19, 2008)
Project PRC2008016 - An investigation into prevalence of Pestivirus infection in cattle and swine herds. Sozzi Dr.ssa Enrica - Capofila - MINSAN - 2008 - 70000,00 EUR - 01/09/2009 0.00.00 31/08/2011 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Sozzi Dr.ssa
Enrica - I
2 - IZSUM - De Mia Dr. Gian Mario - E
First author Canelli, Elena
Bovine viral diarrhoea virus (BVDV) is a Pestivirus which is cause of significant economic losses in cattle
farming. Only 2 distinct genotypes (BVDV-1 and BVDV-2) are recognized and moreover BVDV-1, analysed in
5'UTR, comprises, by now, 15 subgroups (from a to o) [4] and BVDV-2 includes subtypes a and b. The aim of
this study is the subtyping (by sequencing and phylogenetic analysis) of a panel of BVDVs isolated by our
laboratory from 1999 to 2007, in order to find out the distribution of the isolated strains, the most prevalent
subgroups, how recent viruses compare with isolates obtained before and to confront the viruses with others
from Italy and Europe. Methods A panel of 115 samples, preventively tested and found positive to BVDV by
isolation on REB celis (bovine kidney celi), A panel of 115 samples, preventively tested and found positive to
BVDV by isolation on REB celis (bovine kidney celi), were then directly analyzed using RT-PCR for
amplification of a fragment of the 5'-UTR of BVDV, without passages on celi culture. The primer pair used,
BE-B2, targets the 5'UTR, in highly conserved regions of BVDV [2]. They were chosen because of their ability
to differentiate BVDV from other Pestivirus and to determine the genotype of isolates [2]. The PCR products
were sequenced after purification using the same primers of identificative PCR and phylogenetically analysed.
Results Most BVDVs analyzed were classified as genotype 1 and belong to 9 subgroups according to
nomenclature proposed by Nagai et al, 2008. The two groups more represented are BVDV e group (35) and b
group (40). Ali the BVDV-1 b strains create a homogenous group, while BVDV-1 e strains form three separate
genetic groups. The homology among these three groups is around 91-92%, lower than percentages of
similarity between strains belonging to each group (95-97%, 96.5-98.8% and 97% respectively). Other
isolates are grouping as BVDV-1 a, d, f and h (6, 9, 6 and 6 isolates respectively), only 2 as BVDV-1 g and
other 2 as BVDV-1 k. A small group of 5 isolates is lowly genetically correlated to all other genetic subgroups.
Strains of this group showed a high sequence identity between each other (98-99%). No isolates grouped in
BVDV-1 c, i, j, m, n and o. Only 4 BVDV type 2 were detected (3 BVDV-2 b and 1 BVDV-2 a). The
phylogenetic tree reported below was constructed from the 5'UTR sequences used. Sequences of field
viruses analyzed are compared with sequences of reference strains or strains belonging to the on line
GenBank Conclusions The analysis of data obtained, shows that 9 subgroups within BVDV-1 are circulating in
the analysed area, indicating a high level of genetic heterology. BVDV-1 b and 1e are the most frequent
subtypes isolated. On the basis of our sequence comparison results, the group e could be further divided into
three clusters, labelled in this work, for clarity, le1, 1e2 and 1e3, (see figure). Although, these results confirm
partly previous Italian outcomes [1, 3], demonstrating that regional differences in prevailing types do not exist
or are not significant. In this work we describe for the first time two strains belonging to group 1 k, never
described in Italy before and we confirm that the groups BVDV-1 a and g have been present in Italy from
2000, according to other studies [3]. In our study a group is divergent from all others and could represent a
further new group, or it could belong to the group BVDV-11, previously reported by Jackova et al. [5]. Since
we could not find sequences of BVDV-11 in the database, further phylogenetic analysis containing these
isolates was not possible. The low number of BVDV-2 isolates is coherent with the European tendency and
the sporadic Italian occurrence.
ID nr. 3966
Cosciani_Cunico° E, Bonometti° E, Finazzi° G
Pag. 95 di 300 31/08/2011 Validation of Listeria predictive growth model with italian meat product
The 21st international ICFMH symposium : Evolving microbial food quality and safety : 1-4 September, 2008
Aberdeen, Scotland : programme and abstract book / [s.l. : s.n., 2008]. - p 212 - 3 bib ref
International ICFMH symposium (21st : Aberdeen, Scotland : 1-4 September, 2008)
Project RELOPATABIODEC - Bio-decontanimation tools and methods applied to food firms - Boni Dr. Paolo
- Capofila - REGIONE - 2007 - 285064,92 EUR - 01/10/2007 0.00.00 - 30/09/2010 0.00.00 - 1 I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni Dr.
Paolo - I
2 - Gruppo Impresa Finance srl - Tabladini Dr. Marco - E
First author Cosciani_Cunico, Elena
Zampone is a typical Italian food product made with minced pork meat and fat. It is not an RTE food; the precooked slices eaten without or after insufficient heat treatment, is potentially a risk for the consumers. In this
paper, the ability of L.monocytogens to grow in Zampone at refrigeration and abuse temperatures was
studied. Three strains of L.monocytogenes were spread onto slices of pre-cooked Zampone in the
concentrations of ca 10^3 cfu/ml. The contaminated meat slices were then vacuum packed and stored a 4-1015-20°C. The growth of the pathogen was observed by planing it on selective medium at regular intervals. The
samplings were carried out in duplicate: The growth rates were calculated using the model of Baranyi and
Roberts (1994). In pre-cooked Zampone, L.monocytogenes grew at each used temperature values; the
accuracy of the predictive model was of 1.36 (Baranyi et al.1999). The discrepancy between the predictive
model and the experimental data was in the range of what was previously described in literature (Pin et al.
1999). Since Zampone represents a good substrate for L.monocytogenes, to consume this meat without
following the label instruction could be a risk.
ID nr. 3967
Daminelli° P, Monastero° P, Finazzi° G
Techniques for rapid pathogens detection raw milk
The 21st international ICFMH symposium : Evolving microbial food quality and safety : 1-4 September, 2008
Arbeen, Scotland : programme and abstract book / [s.l. : s.n., 2008]. - p 248 - 1 bib ref
International ICFMH symposium (21st : Arbeen, Scotland : 1-4 September, 2008)
Project PRC2005017 - Field surveys (processes and products) and activation of epidemiologic surveillance
protocols of animal breeding for the data supply to the food safety information system - Boni Dr.
Paolo - Solo - MINSAN - 2005 - 290000,00 EUR - 03/07/2006 0.00.00 - 02/07/2008 0.00.00 - 1
- I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni Dr.
Paolo - I
2 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti - Varisco
Dr. Giorgio - I
Recently in Italy the idea regarding "natural food" i very popular among consumers; consequently, according
to Regulation EC No 853/2004, farm increase raw milk commercialisation through automatic dispenser
(Bolzoni et al. 2007). Pathogens like Listeria monocytogenes, Salmonella spp., Campylobactercoli/jejuni, can
be found in raw milk, so it is necessary to organize controls during production and distribution to ensure an
adequate level) of food security for consumers. Lombardia Region is the most important land of Italy for
number and quality of milk farmers, and so the Sanitary Authority organised a monitoring plan to verify
sanitary condition of milk directly sold. Molecular biology methods were applied to obtain results quickly in
order to allow raw milk trading; in particular was used specific real[ time PCR for detection of L. monocytoges
and Salmonella spp., whereas Campylobacter coli/jejuni were detected by . traditional PCR technique.
Microbiology methods were used only to confirm positive results obtained by PCR techniques. The sampling
was made in two period: at first from September 2005 to May 2006, and then from January to August 2007.
1984 raw milk samples collected from 218 milk farms were analyzed by PCR (for all pathogens). The
prevalence of pathogens in raw milk detected by PCR in the first period of sampling was 2,62% for L.
monocytogenes, 1,60% for Salmonella spp., 0,29% for Campylobacter coli/jejuni. the second period the
pathogens prevalence reduced for L. monocytogenes (0,28%) and Salmonella spp (0,70%) while increased
for Campylobacter coli/jejuni (0,88%). Salmonella spp and L. monocytogenes were ìsolated with microbiology
Pag. 96 di 300 31/08/2011 methods respectively only 1 and 5 times while in any time was isolated Campylobacter coli/jejuni. This results
show that the use of PCR methods is very useful because it permits to detect microbiological hazard at low
level, before it becomes critical for health consumers..
ID nr. 3968
Cosciani_Cunico° E, Bonometti° E, Finazzi° G, Daminelli° P
Survival of Salmonella typhimurium in some italian meat products
The 21st international ICFMH symposium : Evolving microbial food quality and safety : 1-4 September, 2008
Aberdeen, Scotland : programme and abstract book / [s.l. : s.n., 2008]. - p 436 - 2 bib ref
International ICFMH symposium (21st : Aberdeen, Scotland : 1-4 September, 2008)
Project RELOPATABIODEC - Bio-decontanimation tools and methods applied to food firms - Boni Dr. Paolo
- Capofila - REGIONE - 2007 - 285064,92 EUR - 01/10/2007 0.00.00 - 30/09/2010 0.00.00 - 1 I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni Dr.
Paolo - I
2 - Gruppo Impresa Finance srl - Tabladini Dr. Marco - E
First author Cosciani_Cunico, Elena
Many of the most well-known Italian meat products are made from raw meat, in which Salmonella
Typtimurium could be present as contaminant (European Rapid Alert System for Food and Feed,
www.ec.europa.eul. In this study, spiced Salame, Salame alla cacciatora, Pancetta arrotolata, Coppa
piacentina, were inoculated with high concentration of pathogen at the begínning of food processing.
Behaviour of S.Typhimurium, temperature, pH, aw, controlling parameters profiles, during food processing
and storage, was studied and the D values were calculated. Three different S.Typhimurium strains were
propagated, the inoculum was collected at the fina) concentration of -10^8 cfu/ml and was spread into the
minced raw meat or over the meat . surface (Hinkens et al. 19961. For each challenge tests, sampling was
carried out in duplicate and enumerations were performed at regular intervals by plating food suspension onto
selective medium. S.Typhimurium had decreased with a linear function depending on the rate of aw
decreasing (Cosciani et al. 2005). In spiced Salame and Salame alla cacciatora, that are commercialized after
21 days of storage, the D value was, respectivety, 4 days and 15 hours with a standard error of 0.51, and 18
days and 7 hours with a SE of 0.32. Storage of Pancetta arrotolata is 50 days and S.Typhimurium D value in
this product was 33 days and 10 hours with a SE of 0.28. Coppa piacentina is usually commercialized after 90
days of storage, and D value of Salmonella on the surface was 17 days and 8 hours with a SE of 0.61. The
process parameters were conform of the commercia) product. Since the considered safe decrease of celi
concentration was not always observed (USDA 2001, 66 FR 125901, we suggest that better control of the raw
materials, the process parameters and the possible longer product storage are necessary in order to
guarantee the safety of these products.
ID nr. 3969
Finazzi° G, Daminelli° P, Cosciani° E, Bonometti° E
Behaviour of Listeria monocytogenes in sliced mortadella stored at different temperatures in
presence of sodium lactate
The 21st international ICFMH symposium "Evolving microbial food quality and safety" : 1-4 September, 2008
Aberdeen, Scotland : programme and abstract book / [s.l. : s.n., 2008]. - p 437 - 2 bib ref
International ICFMH symposium (21st : Aberdeen, Scotland : 1-4 September, 2008)
First author Finazzi, Guido
Listeria monocytogenes is one of the most important microbiological risks in ready to eat food such as
Mortadella (Beumer et a1.1996), an Italian salami, known also as bologna, made with pork meat finely
minced, combined with pork fat cubes and spices and than sacked and cooked at 70°C or more for several)
hours. In this work two kind of Mortadella, regular and added with Sodium Lactate 3.5%, were surface
contaminated with a suspension of three different strains of Listeria monocytogenes in order to obtain a final[
Pag. 97 di 300 31/08/2011 concentration of around 103-104 cfu/cmz (Glass, 2002). Different kinds of Mortadella were then sliced,
packaged in ma condition and stored for 90 days at different temperatures 14, 10, 15 and 20°C) to value the
behaviour of Listeria. In regular Mortadella, stored at 4°C, L.monocytogenes doesn't grow (SE 0.28), while in
Mortadella added with sodium Lactate the pathogen died with a biphasic function with a D value in the first
step of 22 days and 17 hours and a second phase occurring after 38 days in which Listeria population
stabilizes (SE 0.22). At 10°C Listeria increases with a duplication time of 1 day and 17 hours (SE 0.23)
reaching plateau phase after nearly 11 days, while in presence of sodium Lactate the pathogen decreases
with a biphasic function with a D value in the first step of 5 days and 7 hours and after 7 days and 15 hours D
becomes more than 97 days (SE 0.12). At 15°C Listeria increases with a duplication time of 18 hours ± 4
hours reaching plateau phase after nearly 9 days (SE 0.56), while in presence of sodium Lactate the
pathogen population keeps the sure level during the storage period (SE 0.591. Final[y at 20°C the pathogen
increases in both situation but in regular Mortadella the duplication time of Listeria is 8 hours ± 2 reaching'
plateau phase after nearly 3 days (SE 0.43), while in presence of sodium Lactate the duplication time is 1 day
and 17 hours and the plateau phase occurs after nearly 8 days (SE 0.46). After this trial is evident how the
addiction of sodium Lactate during production of Mortadella is useful to inhibit Listeria monocytogenes at
usual storage temperature, and to slow down pathogen replication in situations of thermal abuse.
ID nr. 3971
Blasi G., Lanciotti M, Nardi S., Palombo B, Ferretti E, Di_Giacomo L, Verdecchia E,
Finazzi° G, Pezzotti G
Ciabuscolo : process and product standard evaluation : preliminary study
AIVI Rivista dell’Associazione Italiana Veterinari Igienisti. - Vol. 1 ( 2008). - p 63-64. - 11 bib ref
In this study, samples of "Ciabuscolo", a raw fermented meat product listed among the traditional products of
the Marche Region, were collected from four meat processing plants and analysed to evaluate microbiological
process and product's standards.
ID nr. 3972
Daminelli° P, Finazzi° G, Bertasi° B, Losio° MN, Boni° P, Cosciani_Cunico E
Food safety : veterinary inspection role in food plants
Ind Aliment (Pinerolo). - Vol. 47 no 479 ( 2008). - p 384-389. - 9 bib ref
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Daminelli, Paolo
Food safety is bosed on good knowledge of products and of productive process as well os explained in
Europeon Community Legislation (Reg. CE 2073/2005 integrated by Reg. CE 1441/2007). Food Department
of Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna edited guidelines to support of
food business operators compliance with microbiological criterio and, ot the some time, to explain veterinary
inspection role in food plants.
ID nr. 3973
Pag. 98 di 300 31/08/2011 Finazzi° G, Daminelli° P, Bertasi° B, Monastero P, Boni° P, Cosciani_Cunico E
Dynamic of several pathogens in artificially contaminated fresh and seasoned Caprino cheeses
Ind Aliment (Pinerolo). - Vol. 47 no 484 ( 2008). - p 992-997. - 8 bib ref
Project RELOPATABIODEC - Bio-decontanimation tools and methods applied to food firms - Boni Dr. Paolo
- Capofila - REGIONE - 2007 - 285064,92 EUR - 01/10/2007 0.00.00 - 30/09/2010 0.00.00 - 1 I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni Dr.
Paolo - I
2 - Gruppo Impresa Finance srl - Tabladini Dr. Marco - E
First author Finazzi, Guido
The aim of this trial was to value the behaviour of some pathogens during the processing and ripening of
Caprino, an Italian row goat milk cheese. Different groups of Caprino cheese were made: one as negative
control) and other four were made contaminating the goat mille with suspensions of different strains of
Salmonella yphimurium, Listeria monocytogenes, E. coli O157:H7 and toxigenic Stoph. aureus., with a
concentration of nearly 108 cfu/mL each. The coagulation phase of milk, obtained to 20°-22°C far 24 hours,
wasn't able to determine any effect on pathogens concentration, but ripening the cheese at 6°-8°C for 5-6
days, the combined action of low cheese pH and great amount of lactic bio-competitive flora was able to
determine the rapid decrease of Salmonella (4-5 log) and of E. coli O157:H7 (1 logo), too. To obtain a
decrease of L. monocytogenes population was necessary to extend ripening of the cheese far at least 2
weeks. Staph. aureus capobiliy to produce the toxin was demonstrated tilt the earlier phases of cheese
processing.
ID nr. 3974
Finazzi° G, Daminelli° P, Bonometti° E, Boni° P, Cosciani_Cunico E, Stroppa A
Survival dynamic of Listeria monocytogenes in Grana Padano DOP grated cheese
Ind Aliment (Pinerolo). - Vol. 47 no 480 ( 2008). - p 506-511. - 3 bib ref
Project RELOPATABIODEC - Bio-decontanimation tools and methods applied to food firms - Boni Dr. Paolo
- Capofila - REGIONE - 2007 - 285064,92 EUR - 01/10/2007 0.00.00 - 30/09/2010 0.00.00 - 1 I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni Dr.
Paolo - I
2 - Gruppo Impresa Finance srl - Tabladini Dr. Marco - E
First author Finazzi, Guido
In this challenge test Grana Padano DOP grated cheese was contaminated with a suspension of three
different L. monocytogenes strains in order to value the pathogen population dynamic of different storage
temperature (4°, 10°, 15° and 20'Q. Data observed were compared with theoretical growth curves calculated
applying predictive microbiology models. At 4°C, ideal storage condition, Listeria slowly decreases while in the
predictive model was expected it should increase beginning from 40'h day. At 10°C the pathogen population
doesn't change while was predicted an increase beginning from 151 day. At 15° and 20°C Listeria increases
but slower comparing to what expected. Grano Padano DOP grated cheese, a food with a high amount of
lactic flora able to bio-compete with pathogens, doesn't support the growth of Listeria monocytogenes to ideal
and at usual storage temperature, and is also able to oppose the microbial replication in situation of thermal
abuse.
ID nr. 3975
Finazzi° G, Daminelli° P, Monastero P, Boni° P, Cosciani_Cunico E, Aglietta A
Dynamic of several pathogens on artificially contaminated Valtellina Bresaola PGI
Ind Aliment (Pinerolo). - Vol. 47 no 477 ( 2008). - p 148-153. - 1 bib ref
Project PRF2004201 - Food safety: risk grading systems and process changes toward high-safety products.
- Boni Dr. Paolo - Capofila - MINSAN - 2004 - 239200,00 EUR - 01/04/2005 0.00.00 - 31/12/2006
Pag. 99 di 300 31/08/2011 0.00.00 - 1 - ISS: Centro Nazionale per la qualità degli alimenti e i rischi alimentari - De Medici Dr.
Dario - E
2 - IZSLT: Reparto Microbilogia - Bilei Dr. Stefano - E
3 - IZSME: Laboratorio Alimenti - Bartoli Dr. Mario - E
4 - IZSPLV: Laboratorio Controllo alimenti - Decastelli Dr.ssa Lucia - E
5 - IZSUM: Area Tematica Alimenti - Cenci Dr. Telemaco - E
6 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
7 - IZSPB: Batteriologia Alimentare - Goffredo Dr.ssa Elisa - E
8 - IZSSA: Laboratorio Microbiologia latte e derivati - Fadda Dr. Antonio - E
9 - IZSSI - Santo Dr. Caracappa - E
10 - IZSVE: Laboratorio Batteriologia degli Alimenti - Mioni Dr. Renzo - E
11 - UNICAT: Istituto di Zootecnia - Ajmone Marsan Dr. Paolo - E
First author Finazzi, Guido
The aim of this trial was to evaluate the dynamic of the pathogen population, such as Salmonella typhimurium,
L. monocytogenes and E. coli O157:H7, during the seasoning of traditional Valtellina Bresaola PGI. Different
Bresaola were artificially contaminated on their surface after brine-solting with a 107 cfu/cm2 concentration of
pathogens, and then socked and seasoned. Samples were token from the surface of each bresaola during the
seasoning time of 5 weeks, and analysed to value the populations of pathogens. Salmonella decreased with o
biphasic trend characterized by e first phase in which the decimal decreasing time (D( was 8 hours and 16
minutes, and a second phase in which D become 20 days and 11 hours. However, during the usual seasoning
period, the decrease of Salmonella was equal] to 5 logarithms. E. coli O157:H7 decreased with a line or trend
in which D was 8 days and 20 hours, and during the usual seasoning period, the decrease of the pathogen
was equal to 4 logarithms. The population of L. monocytogenes decreased with a linear trend in which D was
30 days and 17 hours, and so during the usual seasoning period the decrease of the pathogen was less thon
2 logarithms.
ID nr. 3976
Lelli° D, Moreno_Martin° A, Gamba° D, Canelli° E, Sozzi° E, Cordioli° P, Brocchi° E
MAbs based platform for neuraminidase avian influenza viruses typing
Second Annual meeting Epizone : "Need for speed" : 4,5 and 6 - June, 2008 Brescia, Italy : programme and
abstracts / [s.l. : s. n., 2008]. - p 96. - 2 bib ref
Annual meeting Epizone (2nd : Brescia, Italy : 4,5 and 6 - June, 2008)
First author Lelli, Davide
Avian Influenza (AI) is a disease of poultry diffused worldwide that negatively impacts poultry health and
international trade of live animals and their products. Typing of both haemagglutinin (H) and neuraminidase
(N) proteins is crucial to study evolution and epidemiology of Al viruses. The aim of this study is typing of AIVs
using a Mabs based platform able to identify all the N subtypes. Materials and methods AIVs.106 samples of
allantoic fluids infected with AIVs of 14 H (from 1 to 14) and 9 N subtypes were used. Several) AIVs
representative for N1, N2, N3, N4, N7, N9 subtypes were titrated (EID50). Mabs. Mabs specific for 1-9 N were
produced an screened as reported (1). Anti-N MAbs were further analysed in Ni assays versus AIVs of the
nine N subtypes (2). ELISA. Sandwich ELISA for N typing were developed using the same or a combination of
two different Mabs, specific for the required N subtype, as catcher and tracer. In order to valuate their
analytical sensitivity, several ten fold dilution of the titrated strains were analyzed. Results Several hybridomas
were obtained. Combinations of catching and conjugated MAbs specific for each N were identified on the
basis of a wider spectrum of reactivity within homologous N subtypes evidenced by indirect and competitive
ELISA. Ali the MAbs showed subtype-specific M activity. Analytical specificity. Ali 106 AIVs were tested
simultaneously, and reacted specifically, exhibiting a strong signal only in the ELISA test for the corresponding
N subtype. Analytical sensitivity. Serial dilutions of titrateci AIVs were tested. Considering the EID50, the
detection limit for the N-typing ELISAs is calculated to be 105/5,5EID50/01ml. Discussion The rigorous
specificity of the selected MAbs for all N subtypes combined with the capability to recognise all the viruses
examined make the developed N-typing ELISAs appropriate for the rapid identification of AIVs isolated in
allantoic fluids; however, analytical sensitivities are not proper for a direct diagnosis on biological samples.
Pag. 100 di 300 31/08/2011 Great advantages compared with Ni assays derive from the simple and rapid ELISA procedure and from the
use of MAbs, that ensure assays standardisation and reproducibility.
ID nr. 3977
Martelli F, Toma S, Di_Bartolo I, Inglese N, Caprioli A, Ruggeri FM, Lelli° D, Bonci° M,
Ostanello F
Epidemiology and molecular characterization of hepatitis E virus (HEV) strains identified in italian
pigs affected by different pathological conditions
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 257-266. - 27 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
In humans hepatitis E (HE) is an infectious viral disease with clinical and morphological features of acute
hepatitis. The aetiological agent is the hepatitis E virus (HEV). Increasing evidence supports the hypothesis
that HE is an emerging zoonotic disease, and that pigs are the main animal reservoir of the infection Swine
HEV was first identified in 1997 in USA and is now considered to be widespread in pig herds all over the
world. In general HEV in pigs seems to be sub-clinical, although some studies have put in evidence a possible
correlation of the infection with liver damages. Moreover its possible interaction with other pig pathogens such
as porcine PCV2 and PRRSV has still to be fully clarified. In this study we assessed, using a nested-RT-PCR,
the HEV prevalence in 137 pigs of 2-4 months of age from 45 different Italian herds and affected by different
pathological conditions, finding that HEV infection is largely widespread in Italian pigs (29.9% prevalence). We
also performed an evaluation of possible risk factors related to the infection, finding no statistically significant
correlations between HEV positivity and herd typology or presence of concomitant pathological conditions and
coinfections with PRRSV or PCV2. However, the HEV positivity was significantly higher in animals between
80-120 days of age than in pigs <80 days of age (20%). We also performed the molecular characterization of
the identified strains, finding that all of them belonged to genotype 3 and were similar to other European swine
and humans strains.
ID nr. 3978
Ponzoni M, Ferreri AJM, Guidoboni M, Lettini AA, Cangi MG, Pasini E, Sacchi L,
Pecciarini L, Grassi S, Dal_Cin E, Stefano R, Magnino° S, Dolcetti R, Doglioni C
Chlamydia infection and lymphomas : association beyond ocular adnexal lymphomas highlighted by
multiple detection methods
Clin Cancer Res. - Vol. 14 no 18 ( 2008). - p 5794-5800. - 26 bib ref
Impact factor: 6,250
Chlamydia psittaci (Cp) has been associated to ocular adnexal lymphomas (OAL) with variable geographic
distribution. Herein, we used multiple Chlamydia detection tools to identify Cp elementary bodies-containing
cell and to assess Cp prevalence in both nodal and extranodal lymphomas. Experimental Design: TETR-PCR,
immunohistochemistry, immunofluorescence, electron microscopy, and laser-capture microdissection were
done in 35 OALs to define their effect in Chlamydia detection and, moreover, to identify the Cp cellular carrier.
Cp prevalence was screened by TETR-PCR in 205 extraorbital lymphomas and 135 nonneoplasticc controls.
Results: Twenty-six (74%) OALs were associated with Cp infection: immunohistochemistry,
immunofluorescence, and laser-capture microdissection-assisted PCR showed that monocytes/macrophages
were the Cp carriers; electron microscopy showed the presence of intact Cp elementary bodies into these
cells. Immunohistochemistry and TETR-PCR showed a 70%concordance rate (P = 0.001). Cp DNA was
equally prevalent in non-OAL, nodal, and extranodal lymphomas: among the latter, it was more common in
diffuse large B-cell lymphomas of the skin (P = 0.03) and Waldeyer's ring. Conclusions: This multiparametric
approach shows, for the first time, that monocytes/macrophages are the carriers of Cp, Cp seems
preferentially associated with lymphomas arising in organs primarily exposed to antigens. The clinical
implications of these findings deserve to be prospectively investigated.
Pag. 101 di 300 31/08/2011 ID nr. 3979
Ferreri AJM, Dolcetti R, Dognini GP, Malabarba L, Vicari° N, Pasini E, Ponzoni M, Cangi
MG, Pecciarini L, Resti AG, Doglioni C, Rossini S, Magnino° S
Chlamydophila psittaci is viable and infectious in the conjunctiva and peripheral blood of patients
with ocular adnexal lymphoma : results of a single-center prospective case-control study
Int J Cancer. - Vol. 123 ( 2008). - p 1089-1093. - 19 bib ref
Impact factor: 4,555
Ocular adnexal MALT lymphoma (OAML) is linked to Chlamydophila psittaci (Cp) infection. Viability and
infectivity of Cp, demonstrated by growth in culture, has not been yet investigated in these patients. We
conducted a single-center prospective case-control study to assess the prevalence, viability and infectivity of
Cp in 20 OAML patients and 42 blood donors registered in a 6-month period. The presence of Cp in
conjunctival swabs and peripheral blood mononuclear cells CPBMC) of patients and donors was assessed by
TETR-PCR and in vitro cultures. From an epidemiological] point of view, OAML patients often resided in rural
areas, and reported a history of chronic conjunctivitis and prolonged contact with household animals (85% vs.
38% of donors; p = 0.00001). Cp was detected in lymphoma tissue in 15 (75%) patients. Cp DNA was
detected in conjunctival swabs and/or PBMC from 10 C50%) patients and in PBMC from 1 (2%) donor (p =
0.01). Viability and infectivity of Cp, demonstrated by growth in culture, were confirmed in conjunctival swabs
and/or PBMC from 5 (25%) patients, but not in donors (p = 0.002). This prospective study demonstrates, for
the first time, that Cp present in the conjunctiva and PBMC of OAML patients is capable to grow and be
isolated in cell cultures. Cp infection is common in OAML patients and exceptional in blood donors.
Epidemiological data of OAML patients (prolonged contact with household animals and chronic conjunctivitis)
are consistent with Cp exposure risk.
ID nr. 3980
Epis S, Sassera D, Beninati T, Lo N, Beati L, Piesman J, Rinaldi L, Mcoy KD, Torina A,
Sacchi L, Clementi E, Genchi M, Magnino° S, Bandi C
Midichloria mitochondrii is widespread in hard ticks (Ixodidae) and resides in the mitochondria of
phylogenetically diverse species
Parasitology. - Vol. 135 ( 2008). - p 485-494. - 24 bib ref
Impact factor: 2,081
The hard tick Ixodes ricinus (Ixodidae) is the sole animal thus far shown to harbour an intra-mitochondrial
bacterium, which has recently been named Midichloria mitochondrii. The objectives of this work were (ii) to
screen ixodid ticks for Midichloria-related bacteria and (ii) to determine whether these bacteria exploit the
intra-mitochondrial niche in other tick species. Our main goal was to discover further models of this peculiar
form of symbiosis. We have thus performed a PCR screening for Midichloria-related bacteria in samples of
ixodid ticks collected in Italy, North America and Iceland. A total of 7 newly examined species from 5 genera
were found positive for bacteria closely related to M. mitochondrii. Samples of the tick species Rhipicephalus
bursa, found positive in the PCR screening, were analysed with transmission electron microscopy, which
revealed the presence of bacteria both in the cytoplasm and in the mitochondria of the oocytes. There is thus
evidence that bacteria invade mitochondria in at least 2 tick species. Phylogenetic analysis on the bacterial
16S rRNA gene sequences generated from positive specimens revealed that the bacteria form a
monophyletic group within the order Rickettsiales. The phylogeny of Midichloria symbionts and related
bacteria does not appear completely congruent with the phylogeny of the hosts.
ID nr. 3981
Ferreri AJM, Dognini GP, Ponzoni M, Pecciarini L, Cangi MG, Santambrogio G, Resti AG,
Pag. 102 di 300 31/08/2011 De_Conciliis C, Magnino° S, Pasini E, Vicari° N, Dolcetti R, Doglioni C
Chlamydia psittaci-eradicating antibiotic therapy in patients with advanced-stage ocular adnexal
MALT lymphoma
Ann Oncol. - Vol. 19 no 1 ( 2008). - p 194-195. - 5 bib ref
Impact factor: 4,875
ID nr. 3982
Laroucau K, Thierry S, Vorimore F, Blanco K, Kaleta E, Hoop R, Magnino° S, Vanrompay
D, Sachse K, Myers GS, Bavoil PM, Vergnaud G, Pourcel C
High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)
Infect Genet Evol. - Vol. 8 ( 2008). - p 171-181. - 23 bib ref
Impact factor: 2,407
A multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of
Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9 avian
reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for
a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical
origins. Comparative analysis of the MLVA results and those obtained from currently available methods
including serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of
discrimination, with 20 distinct patters identified to date. The newly developed MLVA system therefore
provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different
origins that is suitable for molecular epidemiological studies.
ID nr. 3983
Yousef_Mohamad K, Roche SM, Myers G, Bavoil PM, Laroucau K, Magnino° S, Laurent
S, Rasschaert D, Rodolakis A
Preliminary phylogenetic identification of virulent Chlamydophila pecorum strains
Infect Genet Evol. - Vol. 8 ( 2008). - p 764-771. - 52 bib ref
Impact factor: 2,407
Chlamydophila pecorum is an obligate intracellular bacterium associated with different pathological conditions
in ruminant, swine and koala, which is also found in the intestine of asymptomatic animals. A multi-virulence
locus sequence typing (MVLST) system was developed using 19 C. pecorum strains (8 pathogenic and 11
non-pathogenic intestinal strains) isolated from ruminante of different geographical origins. To evaluate the
ability of MVLST to distinguish the pathogenic from the non-pathogenic strains of C. pecorum, the sequences
of 12 genes were analysed: 6 potential virulente genes (ompA, incA, incB, incC, mip and copN), 5
housekeepinggenes (recA, hemD, aroC, efp, gap), and the ORF663 gene encoding a hypothetical protein
(HP) that includes a variant 15-nucleotides coding tandem repeat (CTR). MVLST provided high discriminatory
power (100%) in allowing to distinguish 6 of 8 pathogenic strains in a single group, and overall more
discriminatory than MLST targeting housekeeping genes. ompA was the most polymorphic gene and the
phylogenetic tree based only on its sequence differentiated 4 groups with high bootstrap values. The number
of CTRs (rich in serine, proline and lysine) in ORF663 detected in the pathogenic strains was generally lower
than that found in the intestinal strains. MVLST appears to be a promising method for the differential
identification of virulent C. pectorumstrains, and the ompA, incA and ORF663 genes appear to be good
molecular markers for further epidemiological investigation of C. pecorum.
ID nr. 3984
Magnino° S, Haag-Wackernagel D, Geigenfeind I, Helmecke S, Dovc A, PruknerRadovcic E, Residbegovic E, Ilieski V, Laroucau K, Donati M, Martinov S, Kaleta EF
Pag. 103 di 300 31/08/2011 Chlamydial infections in feral pigeons in Europe : review of data and focus on public health
implications
Vet Microbiol. - Vol. 135 ( 2009). - p 54-67. - 109 bib ref
Impact factor: 2,370
First author Magnino, Simone
Feral pigeons (Columba livia domestica), which thrive in most European towns and cities, are commonly
infected with the zoonotic bacterium Chlamydophila psittaci, the agent of psittacosis (also known as
ornithosis) in humans. A number of surveys carried out over the last thirty years across Europe have detected
high seropositivity values and high percentages of infection in feral pigeon populations. Overall, when
considering data from 11 European countries, seropositivity values to C. psittaci in the sampled
populationranged from 19.4% to 95.6%. In most surveys, the complement fixation test was used, and
antibodies were detected in 19.4-66.3% of the samples, with a median of 46.1%. Indirect immunofluorescence
and ELISA tests were employed less frequently, but led to the detection of higher percentages of
seropositivity (23.7-67.7% and 35.9-95.6%, respect lively. Attempts to grow C. psittaci in cell culture or
embryonated chicken eggs were successful in 2-42.3% and 0-57.1% of samples, respectively, antigen
detection methods were positive in 2.3-40% of samples, while conventional PCR and real-time PCR using
different genomic targets detected the organism in 3.4-50% of samples. Twenty-five C. psittaci isolates from
pigeons were typed as ompA genotype B (n = 14), E (n = 10) and E/B (n=1). The huge increase of feral
pigeon populations in Europe is a major cause of concern for the detrimental effect of pigeon droppings on
environmental hygiene, in addition to the extensive damage due to the fouling of buildings and monuments.
The most important pathogenic organism transmissible from feral pigeons to humans is C. psittaci, with 101
cases of disease reported in the literature. Exposure to C. psittaci-contaminated dust, direct contact with
pigeons through handling and, to a lesser extent, through pigeon feeding have been identified as hazardous
exposures in more than half of the human cases, while loose or transient contacts with feral pigeons have
been mentioned in about 40% of the cases. Education initiatives as to the communication of a health risk
resulting from contact with pigeons and pigeon excreta should primarily be targeted at individuals who may be
exposed to C. psittaci-contaminated dust, such as demolition/construction workers. Recommendations to this
category of workers include wearing protective clothes with hoods, boots, gloves and air filter face masks
when removing pigeon faeces from roofs, garrets and buildings, especially if working indoors. Monitoring for
C. psittaci infections in these workers over time should also be considered. Children should be warned not to
handle sick or dead pigeons, and immunocompromised individuals should be advised to carefully limit their
contact to feral pigeons. Culling of pigeons by shooting or poisoning is both unethical and ineffective as the
place of the killed birds in the population is quickly filled by new juveniles or immigrating birds from
neighbouring areas. Pigeon-deterring systems, such as nets and plastic or metal spikes applied to buildings
and monuments will prevent their fouling, and the administration of contraceptive drugs may allow size
regulation of the pigeon populations. Nevertheless, the measure that will ultimately lead to permanent
reduction and will establish healthy sustainable populations is the restriction of indiscriminate feeding by
pigeon lovers. The erection of dovecotes and artificial breeding facilities should be considered for providing
shelter and a balanced diet to the birds, as well as a chance of interaction for pigeon lovers in a hygienically
controlled environment.
ID nr. 3985
Brocchi° E, Grazioli° S, Pezzoni° G, Borrè° A
Confirmatory tests for Foot-and-Mouth disease DIVA serology based on profiling of antibodies to
non structural proteins
Second Annual meeting Epizone : "Need for speed" : 4,5 and 6 - June, 2008 Brescia, Italy : programme and
abstracts / [s.l. : s. n., 2008]. - p 125. - 1 bib ref
Annual meeting Epizone (2nd : Brescia, Italy : 4,5 and 6 - June, 2008)
Project SSPE-CT-2003-503603 - Improvement of foot and mouth disease control by ethically acceptable
methots based on scientifically validated assays and new knowledge on FMD vaccines, including the
impact of vaccination (FMD-IMPROCON). - De Clercq Dr. Kris - UO - CE - 2003 - 2399907,00
EUR - 01/01/2004 0.00.00 - 31/12/2008 0.00.00 - 1 - H1 - Reparto di Biotecnologie - Brocchi
Dr.ssa Emiliana - I
Pag. 104 di 300 31/08/2011 PRC99004 - Development and validation of innovating serological methods for the differentiation of
vaccinated from FMD-virus infected animals based on the use of multiple non-structural antigens Brocchi Dr.ssa Emiliana - Solo - MINSAN - 1999 - 16/08/2000 0.00.00 - 15/08/2002 0.00.00 142524000,00 LIT 1 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
First author Brocchi, Emiliana
The vaccinate-to-live policy for foot-and-mouth disease (FMD) is based on the availability of validated
screening assays to differentiate vaccinated from infected animals based on the detection of antibodies to
FMDV non structural proteins (NSP). The need of confirmatory tests for NSP-serology has encouraged this
study for the determination of serum profiles against multiple NSP.
Materials and Methods: Indirect ELISAs for the measurement of antibodies to the NSP 3A, 313 and 3D of
FMDV were developed using monoclonal antibodies to trap the respective recombinant antigens. Diagnostic
performances were studied using known sera derived from experimental cattle with a know FMD status. Data
were analysed in comparison with results of a validated 3ABC-trapping ELISA (1).
Results and discussion: Early seroconversions against 3A, 313 and 3ABC were similar, while antibodies to 3D
occurred two days earlier; however, antibodies to 3B were low or not detectable in few animals. Duration of
antibodies was evaluated up to 400 days alter infection: results showed that antibodies detected by the 3ABCELISA were the most persistent, followed by antibodies detected by 3A-ELISA, while antibodies detected by
313- and 3D-ELISA decayed more rapidly. Specificity for 3A-ELISA was 98.3%, sensitivity reached 100% in
infected cattle and 83% in cattle that were vaccinated prior to infection. These sensitivity estimates are similar
to those found for the validated 3ABC-trapping ELISA, consistently with the good correlation observed
between the two tests. The specificity for 3B-ELISA reached 99%, whilst sensitivity was significantly lower in
both categories of infected (75%) and vaccinated/infected (72%) cattle, possibly due to either "3B-nonresponder" cattle or to a faster decay of antibodies to 313. Further research is needed to improve sensitivity
and specificity of the 3D-trapping ELISA.
Conclusions: 3A- and 3B-ELISA may be considered as prototype NSP-ELISAs useful for NSP-Ab profiling.
They do not reach individually the diagnostic performance of the 3ABC-ELISA; however, combined in a
"profiling test" comprising multiple antigens, they may have interesting applications, included a potential use
as confirmatory system.
ID nr. 3987
Dottori° M, Bonilauri° P, Merialdi° G, Nigrelli° A, Martelli R
Monitoring chronic pleuritis due to Actinobacillus pleuropneumoniae at slaughterhouse by a newly
implemented scoring system
20th Internationl Pig Veterinary Society Congress IPVS 2008 : Durban, South Africa, 22-26 June 2008 :
poster proceedings / [s.l : s.n, 2008]. - p 230. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (20 : Durban, (South Africa) : 22-26 June 2008)
First author Dottori, Michele
The paper proposes a newly implemented grid to score CP lesions and shows the preliminiary result of its
application in Italian pigs at slaughterhouse.
ID nr. 3988
Rosignoli° C
Respiratory disease in newly received feeder cattle: risk reduction strategies
I° Congresso europeo SIVAR : Nuovi orizzonti per la veterinaria europea : salute, benessere animali e
produzioni di qualità : New perspectives for european veterinary profession : animal health, welfare and
quality productions : VeronaFiere 8-9 Febbraio 2008 / [s.l : s.n, 2008]. - p 52-56
Congresso europeo SIVAR (1 : VeronaFiere : 8-9 Febbraio 2008)
First author Rosignoli, Carlo
Pag. 105 di 300 31/08/2011 Bovine respiratory disease complex (BRDC) is caused by the pathogenic action of various micro-organisms
(viruses, bacteria and mycoplasmas) but also, to a large extent, by the simultaneous presence of risk factors,
such as environmental and management stress, that reduce respiratory defences or increase exposure to
pathogens. In beef cattle fattening herds there are many risk factors for BRDC such as mixing animals from
different origins, transport over large distances and reduced space for fattening process. These conditions
explain why respiratory diseases occur mainly in the first four weeks after the arrival of animals, which is when
the negative consequences of the main stressful events are concentrated. Strategies to reduce the risk of
respiratory disease in newly received feeder cattle must consider three areas of action: the maintenance of
disease resistance, minimisation of exposure to infectious agents and reduction of stress. In this paper we
look at the critical phases of the productive process in order to control and reduce outbreaks of respiratory
diseases in feeder cattle.
ID nr. 3989
Losio° MN, Bertasi° B, D'Abrosca° F, Pavoni° E, Curatolo° M, Boni° P
In vitro toxicology methods for the evaluation of the efficacy of functional food
Ind Aliment (Pinerolo). - Vol. 47 no 482 ( 2008). - p 758-762
Project PRC2003004 - Evaluation of bio-toxigenicity in functional foods and food integrators for humans. Bertasi Dr.ssa Barbara - Solo - MINSAN - 2003 - 60000,00 EUR - 26/04/2004 0.00.00 25/10/2005 0.00.00 - 1 - L1 - Centro Substrati Cellulari - Bertasi Dr.ssa Barbara - I
First author Losio, Marina Nadia
“Food as benefic product" is a recent concept shared by science and consumere. Recently, it has been a
relevant increase in the trading of food meant as well-being promoter. At present, European low has a
shortage about the reguiation of food proposition as wellness proposer, but many parts of food trading and
production are asking for new rules oble to protect the characteristics that define each ype of product. Cell
culture and in vitro toxicology-alternative methods are important means to detect the activity of some specific
molecules on the cellular behoviour, so they are the scientific tools to rely on for the proposition of bills
directed to warrant the food sofety. On this bases, they will be described some possible cell culture
applications and in vitro toxicology tests, to screen the most common properties of Iunctional foods such as
probiotic activity, anti-oxidant and immunomodulating activty.
ID nr. 3992
Bertasi° B, Agnelli° E, Pavoni° E, Daminelli° P, Boni° P
Application of a Multiplex PCR for the detection of Escherichia coli O157:H7 in food
Ind Aliment (Pinerolo). - Vol. 47 no 478 ( 2008). - p 276-280. - 19 bib ref
First author Bertasi, Barbara
Escherichia coli 0157:H7 is considered an emerging pathogen (WHO) and frequently responsible of serious
clinical disease, as for example hemorrhagic colitis. In general infection risk is thought to be related to
contominated food. It is therefore necessory to standardize and improve methods with high specificity and
sensitivity, in order to control different type of food. In this paper it is described the set-up of a PCR technique
with high sensitivity (limit of detection: 102 ufc/g) and specific for virulence and adherence of E. coli 0157: H7;
the selected PCR was used for food screening to estimate the related real risk.
ID nr. 3993
Bellini° S, Alborali° L, Bonazza V, Avisani° D, Zanardi° G
Swine vesicular disease in Lombardy Region: diffusion in densely populated pig area
Pag. 106 di 300 31/08/2011 Second Annual meeting Epizone : "Need for speed" : 4,5 and 6 - June, 2008 Brescia, Italy : programme and
abstracts / [s.l. : s. n., 2008]. - p 79
Annual meeting Epizone (2nd : Brescia, Italy : 4,5 and 6 - June, 2008)
Project EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Bellini, Silvia
Swine vesicular disease (SVD) is a contagious disease of pigs caused by an Enterovirus of the Picornaviridae
family. In Europe, SVD outbreaks have been steadily reported in Italy in the last decade, where surveillance
and eradication activities are in piace. In the period 2006 - 2007 a recrudescence of SVD was recorded and
the disease spread widely in the Italian Northern Regions. Lombardy, a region with high pig stoking density,
was most affected. Even though SVD is considered to be moderately contagious, the 2006 - 2007 epidemic in
Lombardy, compare to the previous ones (1989-1989, 2000, 2002), was characterized by a rapid disease
spread. In fact, 53 outbreaks were detected and some 150.000 pigs were stamped out. The SVD outbreaks
reported in Lombardy in the period 2006 - 2007 may be grouped in two epidemic periods. During the first one
(November 2006 - February 2007) SVD spread among the farms according to the typical routes of
transmission of the disease. On the contrary, in the second epidemic period (May 2007 - October 2007), the
diseases showed an endemic trend in a small high-density area of the region. The main risk factor for
outbreaks in this area, was proximity to a previous outbreak. To verify the pattern of SVD spread in highdensity-areas and to highlight the relevant risk factors, the epidemiological investigations and bio-security
questionnaires carried out in the outbreaks were thoroughly evaluated.
ID nr. 3994
Pisoni G, Locatelli C, Alborali° L, Rosignoli° C, Allodi S, Riccaboni P, Grieco V, Moroni P
Outbreak of Nocardia neocaledoniensis mastitis in an italian dairy herd
J Dairy Sci. - Vol. 91 ( 2008). - p 136-139. - 16 bib ref
Impact factor: 2,361
Nocardia spp. are an uncommon cause of mastitis, and outbreaks have typically been reported in dairy farms
with poor hygienic and management conditions. The outbreak described herein involved a dairy farm with 43
lactating cows that, after a long period with low bulk milk somatic cell counts «180,000 cells/mL), experienced
an increasing incidente of clinical mastitis with bulk milk somatic celi counts greater than 300,000 cells/mL.
Fifteen mastitic quarters milk samples from 9 dairy cows were found to be infected by a member of the genus
Nocardia, as identified on the basis of selected phenotypic and chemotaxonomic characteristics. The isolates
were confirmed as Nocardia neocaledoniensis by 16S rDNA gene sequencing. Average quarter milk somatic
cell count for infected udders was 863,057 cells/mL, signíficantly greater than the average value in
noninfected quarters (189,710 cells/mL).
ID nr. 3996
Pavoni° E, Losio° N, Colombrita D, Foresti I, Caruso A, Suffredini E, Croci L
Detection of Norovirus in stool samples of hospitalized patients with gastroenteritis
Symposium “Current developments in food and environmental virology" : 9-11 October 2008, Pisa (Italy) /
[s.l. : s.n., 2008]. - p 62
Symposium “Current developments in food and environmental virology" : Pisa (Italy) : 9-11 October 2008)
Project ENVIRONET COST 929 - A European Network for Environmental and Food Virology (Environet) Cook Dr. Nigel - UO - CE - 2006 - 0,00 EUR - 21/09/2006 0.00.00 - 12/11/2010 0.00.00 - 18 I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Losio
Dr.ssa Marina Nadia - I
Pag. 107 di 300 31/08/2011 First author Pavoni, Enrico
Norovirus (NoV) is one of the main causes of children and adults acute gastroenterttis worldwide. NoVs,
belonging to the Caliciviridae family, are divided in five genogroups (GI io GV), based on sequence
homologies in their RNAs. GI and Gli, that are the most commonly described in human infections, are further
subdivided into more than 25 different genotypes. The ORF2 major capsid protein sequence can diverge by
as much as 60% between genogroups and 20-30% between genotypes within a genogroup. Thís
classification is in constant evolution with the discovery of new variant strains. Italy, unlike other countries, has
no specific surveillance system for virai gastroenteritis then few epidemiological data are availabie regarding
NoV gastroenteritis. Thz objective of this study was to evaiuate the involvement of NoVs in patients with
gastroenteritis from different settiements (a Civil Hospital in Brescia and a Retirement Home in Ferrara). One
hundred thirty three stool samples, negative for routine bacteriological tests, were analysed for norovirus
presence first by ELISA (Oxoid) and then by molecular detection (conventional two round PCR and Real Time
PCR). Circulating strains were characterized by sequencing of PCR ampiicons and identification through
BLAST search and comparison through the European Foodborne Viruses Database. A total of sixty-three
stool samples (47%) resulted positives for the presence of norovirus. The sequencing showed the presence of
G11.4, Gll.b, G11.7 and GIA, among these GI1.4 being largely more frequent than the others..
ID nr. 3997
Suffredini C, Corrain C, Arcangeli G, Fasolato L, Manfrin G, Rossetti E, Biazzi E, Mioni R,
Pavoni° MN, Losio° MN, Sanavio G, Croci L
Occurrence of enteric viruses in shellfish and relation to climatic-environmental factors
Lett Appl Microbiol. - Vol. 47 ( 2008). - p 467-474. - 50 bib ref
Impact factor: 1,623
Aims: To investigate the presence of enteric viruses [hepatitis A (HAV) and norovirus (NoV)] in shellfish
harvested from the deltaic area of the Po river in relation to environmental factors. Methods and Results:
Fortnightly sampling of shellfish was carried out in two lagoon areas (category B production areas) and one
sea area (category A). Environmental parameters in the lagoon and hydrometric level) of the tributary river
were monitored throughout the sampling period. Samples (n = 120) were analysed for bacterial (E. coli and
Salmonella) and viral (HAV and NoV) con termination; samples from category B areas were analysed before
and after pure fiction treatment. All the samples were negative for HAV whereas 10 samples (8.3%), all
harvested in the lagoon areas, were positive for NoV. Sequencing identified the strains as genotypes II.4 and
II.b. None of the samples was found to be contaminated after depuration. Conclusions: The monitoring
showed a low frequency of NoV presence; viral contamination, detected exclusively in shellfish collected from
the deltaic area (category B), could be influenced by the flow of the tributary river. Significance and Impact of
the Study: The data collected are useful for the design of targeted prevention strategies and for the
modulation of control plansafter metereologicla events.
ID nr. 3998
Gelmetti° D, Giudice C, Invernizzi° A, Bertoletti° I
Bilateral ocular disgenesis in young deer (Cervus elaphus)
Atti 5 congresso nazionale Associazione italiana di patologia veterinaria (AIPVet) : 15-16 Maggio 2008
Mondello (PA) / [s.l. : Associazione italiana di patologia veterinaria, 2008]. - p 55
Congresso nazionale AIPVet (5. : Mondello (PA) : 15-16 Maggio 2008)
First author Gelmetti, Daniela
A young male deer, about 3 day-old was found dead in Campodolcino (Sondrio-Italy). The animai was
affected by bilateral microphthalmus (1.5 cm in diameter), diffuse corneal opacity, eyelid coloboma at the
nasal cantus, conjunctival prolapse without conjunctival secretion. No other external gross alteration was
detected and necropsy revealed no internal organs lesions. Bacteriological tests (perfonned ori lung, live,
Pag. 108 di 300 31/08/2011 kidneys and spleen) and parasitological examination were all negative. Ocular globes were formalin fixed
paraffin embedded. Histologically both eyes have similar alterations. A large dermoid cyst, composed by
squamous epithelium, sebaceous glands and hair follicles, expanded anterior chamber and partially infiltrated
and distorted iris stroma. A mass composed of spindle to stellate cells and small scattered tubules, embedded
in a mixoid extracellular matrix, expanded vitreal chamber. Complete retinal detachment and corneal fibrosis
and vascularization were also visible. Immunoassaying of vitreal mass (pan-cytokeratins, Vimentin, Gfap,
NSE, MAP2, neurofilaments, alfa smooth muscle actin, S100) revealed both neural and mesenchymal
elements. The presence of structures of different embryonal histogenesis was consistent with complex
microphthalmia described in human medicine.
ID nr. 3999
Marone P, Fabbi° M, Daffara S, Della Valle C, De_Vitis D, Mariani B, Brunetti e, Andreoli°
G, Vicari° N
The cat scratch disease: a rare zoonoses?
XXXVII Congresso Nazionale Associazione Microbiolologi Clinici Italiani (AMCLI) : 5-8 Ottobre 2008 Stresa /
[s.l. : Associazione Microbiolologi Clinici Italiani, 2008]. Congresso Nazionale Associazione Microbiolologi Clinici Italiani (AMCLI) (27 : Stresa : 5-8 Ottobre 2008)
Project PRC2003013 - Development and application of diagnosis and therapy protocols for Cat Scratch
Disease (CSD) in human and veterinary medicine, monitoring of disease prevalence in humans and
of Bartonella henselae in Italian feline populations. - Fabbi Dr. Massimo - Capofila - MINSAN 2003 - 45000,00 EUR - 26/04/2004 0.00.00 - 25/10/2006 0.00.00 - 1 - M8 - Pavia - Fabbi Dr.
Massimo - I
2 - IRCCS S. Matteo laboratorio di Batteriologia e Micologia - Area Infettivologica - Marone Dr.
Piero - E
Cat-scratch disease (CSD) is a zoonotic emerging disease caused by Bartonella henselae. Cat is the natural
reservoir of the microorganism in which the infection is usually asintomatic. Over 8 months of investigation a
total of 16 cases have been observed. The age of patients ranges from 5 to 75 years (in 10 cases <_ 20), 9
females and 7 males. In ali cases there was a lymphadenopathy (axillary 9, inguinal 4, lateral cervical 3), fever
was reported in 9 cases, 2 patients had a maculopapular rash. Serology was positive in ali cases observed.
The fine needle aspiration of the lymph node under ultrasound guidance was performed in 4 patients, the
culture of the sample was negative, while PCR was positive in 2 cases. Twelve patients underwent antibiotic
therapy, in most cases with a macrolide (clarithromycin). A survey on domestic cats have shown that 35/165
(23%) were bacteraemic and 49/165 (29%) were seropositive. 45% were infected with B.henselae type I, 36%
type li, 12% from both serotypes and 6% from B.clarridgeiae.
ID nr. 4000
Mazzoni C, Bonilauri° P, Borri E, Tonon F, Ponzio M
Field experience on farrowing room efficiency improvement in herd with two-week forrowing system
20th Internationl Pig Veterinary Science Congress IPVS 2008 : Durban, South Africa, 22-26 June 2008 :
poster proceedings / [s.l : s.n, 2008]. - p 604. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20 : Durban, (South Africa) : 22-26 June 2008)
This paper reports a field experience on a farm that achieved a considerable farrowing room efficiency
improvement using a methodical work of farrowing synchronization and assistance.
ID nr. 4001
Bassi° S, Carra° E, Carpana E, Ferro A, Pongolini° S
Pag. 109 di 300 31/08/2011 Detection of Paenibacillus larvae spores in naturally and artificially contamined honey samples :
comparison between cultural and molecular methods
Symposium OIE- APIMONDA Diagnosis and control of bee diseases : August 26 and 28, 2008 Freiburg
(Germany)" / [s.l. : s.n., 2008]. - p 136-137
Symposium OIE- APIMONDA : Freiburg (Germany) : August 26 and 28, 2008)
First author Bassi, Stefano
American foulbrood is one of the most severe and widespread diseases of the honeybee. The causative agent
of the disease is the spore forming bacterium Paenibacillus larvae. Honey produced by infected honeybee
colonies becomes contaminated by the spores of this agent. The assessment of honey contamination makes
it possible to infer the severity of colony exposure to P. larvae. In particular, the discrimination of contaminated
from non contaminated honey samples helps differentiating between potentially diseased and non diseased
honeybee colonies. Bacterial culture with colony counting and confirmation is the reference method for P.
larvae spores assessment in honey. However this method suffers from long incubation and processing time.
The aim of our study was the evaluation of a 16S rDNA PCR based method as a faster alternative to culture in
the screening of honey samples for the presence of P. larvae spores. Specifically, PCR was compared to
bacterial culture in order to determine its capability to correctly discriminate non contaminated from
contaminated samples with different spore load.For the purpose of this study several naturally occurring and
artificially contaminated honey samples were comparatively tested by bacterial culture and PCR. Our
contribution reports the results of the study.
ID nr. 4002
Bonilauri° P, Bellini R, Calzolari° M, Angelini R, Venturi L, Fallacara° F, Cordioli° P,
Angelini L, Venturelli C, Merialdi° G, Dottori° M
Chikungunya virus in Aedes albopictus, Italy
Emerg Inf Dis. - Vol. 14 no 5 ( 2008). - p 852-853. - 8 bib ref
Impact factor: 5,775
First author Bonilauri, Paolo
not found.
ID nr. 4004
Calzolari° M, Bonilauri° P, Mascali_Zeo S, Bellini R, Angelini P, Venturelli C, Dottori° M
Screening for viral dectection of Aedes albopictus larvae hatched from eggs collected during a
Chikungunya outbreak in Italy
Second Annual meeting Epizone : "Need for speed" : 4,5 and 6 June, 2008 Brescia, Italy : programme and
abstracts / [s.l. : s. n., 2008]. - p 110. - 3 bib ref
Annual meeting Epizone (2nd : Brescia, Italy : 4,5 and 6 June, 2008)
First author Calzolari, Mattia
In 2007 the mosquito Aedes albopictus was involved as vector in an outbreak of CHIK in Italy. The disease
affected about 250 people, mainly in the village of Castiglione di Cervia (Ravenna). The risk of overwintering
of the CHIKV is represented by infected females transmitting the virus into the eggs (VT). Experiments
conducted on VT of CHIKV in Ae. albopictus produced conflicting results: it was excluded by Mourya (2), but
Zhang et al. (3) isolated the virus from pools of Tiger mosquitoes born from experimentally infected mothers.
Recently, the CHIKV was isolated in 2 out of 500 pools of larvae sampled in La Reunion (1). The aim of this
study was to check the presence of CHIKV in the mosquitoes progeny belongin to the area of the Italian
outbreak. 2.Material and Methods Eggs from ovitraps employed for monitoring of Tiger mosquito were
collected. The ovitraps sticks were put in closed glass jars with appropriate solution and let at room
Pag. 110 di 300 31/08/2011 temperature for 12-14 h, in order to hatch the eggs. The same sticks after first hatching were conserved for
further 3-4 weeks at 4-5°C and re-processed at least other two times, to hatch diapausing eggs. Larvae were
reared at room temperature for 7-10 days, grouped in 15-20 individuals pools and submitted to RTPCR3.
Results 402 sticks sampled from August until October 2007 in 158 sites were processed. A total of 8947
specimens (16% from diapausing eggs) was obtained, 101 were from Castiglione. The specimens obtained
were grouped into 590 pools and subjected to PCR, without any positive result. 4. Discussion and Conclusion.
No positive PCR results were obtained, indicating that VT didn't occur in the sampled eggs. Anyway VT is a
rare event in CHIK and it might not be easily detectable. For this reason our thought is that re-appearing of the
disease from Ae. albopictus larvae is improbable but this event can not be completely excluded.
ID nr. 4005
Fallacara° F, Bonialuri° P, Sambri V, Rossini G, Cavrini F, Cordioli° P, Lavazza° A, Bellini
R, Calzolari° M, Angelini R, Venturi L, Angelini° P, Venturelli C, Pacciarini° ML, Merialdi°
G, Dottori° M
Complete nucleotide sequence of Italian Chikungunya virus
Second Annual meeting Epizone : "Need for speed" : 4,5 and 6 - June, 2008 Brescia, Italy : programme and
abstracts / [s.l. : s. n., 2008]. - p 108. - 3 bib ref
Annual meeting Epizone (2nd : Brescia, Italy : 4,5 and 6 - June, 2008)
First author Fallacara, Francesca
introduction and Objectives in this work, we describe the complete sequence of Chikungunya virus (CHIKV)
detected in Aedes albopictus (1) collected during the Italian outbreak observed in summer 2007 (ITA07-RA1,
GenBank EU244823) and the E1 sequences of CHIKV isolates from human sera. 2.Material and Methods
Complete nucleotide sequence was performed employing primers previously described (2). Phylogenetic
analysis was performed with MEGA 4 (3) using the neighbor-joining algorithm and the Kimura 2-parameter
distance model. 3.Results The CHIKV genomic RNA was 11788 nt long. The comparison with other CHIKV
strains showed that ITA07-RA1 clustered with Indian isolates (IND-06, 99.5% nt identity) and Reunion Islancis
isolates (RU, 98.8% nt identity), into the ECSA genotype. Analysis of partial E1 (1044 bp, from 10264 to
11307 according to 527 strain) from mosquitoes and human sera shows 100% nt identity. In E1 region, the
Italian strain shows two relevant changes (A226V and D284E), also present in >90% of later RU isolates. It
was hypothesized that these mutations confer evolutionary success to the virus in its adaptation to the urban
vector A. albopictus (2). Moreover, ITA07-RA1 presents the two unique substitutions noted in all of the IND06
isolates, but not in the RU isolates, both in Nspl region (T128K and T376M). 4.Discussion and Conclusions.
The Italian CHIKV sequenced from mosquitoes and human isolates shows 100% identity. This finding may
confirm the role of A. albopictus as vector of the virus in the Italian outbreak (1). Moreover, in the Italian CHIK
strain the two relevant substitutions (A226V and D284E) noted in all of the IND06 isolates - but not in the RU
isolates - are present. This finding may indicate that the Italian virus comes from India where the CHIKV and
the disease are still endemic. This episode indicates that Italy is at risk of introducing arboviruses causing
human diseases, such as Dengue, West Nile and Rift valley fever.
ID nr. 4006
Muraro M, Solari_Basano F, Nazzari R, Franzini° G
Efficacy of Tulathromycin in the prevention of bovine respiratory disease (BRD) ira stocker cattle in
Italy
Large Anim Rev. - Vol. 14 ( 2008). - p 267-272. - 11 bib ref
A randomised clinical study was conducted to evaluate the efficacy of Tulathromycin in the prevention of
bovine respiratory disease (BRD), in typical Italian breeding conditions. Por the enrolment in the study 214
male cattle, weighing 357 to 546 kg, were imported from France. At arrival, all the animals were vaccinated,
dewormed and managed as per normal farm practices. In the first days following the arrival of the animals to
the farm, the BRD outbreak was confirmed by clinical and microbiological assessments presence of R
Pag. 111 di 300 31/08/2011 multocida, H. somni e M. bovis) on 18 animals with respiratory signs, then, all the animals were treated with
Tulathromycin (G1 = 96) or Tilmicosin (G2 = 97) in commercial formulations and following the label
instructions. In the two weeks following the treatment, Tulathromycin showed an high efficacy in BRD
prevention (97,8%), significantly higher than Tilmicosin (89,1%, p = 0,0326). The comparison of daily weight
gain of the two groups showed an overall improvement of production performance of the animals treated with
Tulathromycin (average body weight = 537 kg, IPG = 1,69 kg/day) when compared to those treated with,
Tilmicosin (average body weight = 530 kg, IPG = 1,57 kg/day). The lack of statistical significance of this data
suggest further investigation by increasing the sample size.
ID nr. 4007
Makoschey B, Beer M, Zientara S, Haubruger E, Rinaldi L, Dercksen D, Millemann Y, Rijn
PV, De_Clerq K, Oura C, Saegerman C, Domingo M, Sanchez_Vizcaino JM, Mehlhorn H,
Tamba° M, Thiry E
Bluetongue control : a new challenge for Europe
Berl Muench Tieraerztl Wochenschr. - Vol. 121 no 7/8 ( 2008). - p 306-313
Impact factor: 0,721
Project PRC2004003 - Epidemiologic and entomologic investigations for the control of Blue tongue disease
(BT) in an area which is free from the disease but endemic for a BT virus of vaccinal origin - Tamba
Dr. Marco - Solo - MINSAN - 2004 - 63020,00 EUR - 01/07/2005 0.00.00 - 30/06/2007 0.00.00 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Reggio Emilia - Dottori Dr. Michele - I
3 - N4 - Modena - Pongolini Dr. Stefano - I
4 - N3 - Forlì - Massi Dr.ssa Paola - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
not found.
ID nr. 4008
Lavazza° A, Cardeti G
Bee viral infections and mortality of colonies
Spopolamento e morte di alveari : aspetti sanitari : 18 - 19 Giugno 2008 Roma / [s.l. : Istituto Zooprofilattico
Sperimentale delle Regioni Lazio e Toscana, 2008]. - p 57-69. - 13 bib ref
Spopolamento e morte di alveari : aspetti sanitari : Roma : 18 - 19 Giugno 2008)
Project PRC2007110 - Epidemiological study on notifiable bee diseases and evaluation of the relative
sanitary legislation - Formato dr.Giovanni - UO - MINSAN - 2007 - 4950,00 EUR - 01/12/2008
0.00.00 - 30/11/2010 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza
Dr. Antonio - I
First author Lavazza, Antonio
Specific viruses affecting honeybees are widespread and normally give latency i.e. they can be detected in
seemingly healthy bees. In the last decade we investigated the presence and spread of bee viruses in Italian
hives. Samples of honeybees at different stages were examined by electron microscopy methods, including
IEM for ABPV and ELISA test for DWV. DWV and ABPV were often detected in high titres in association with
severe signs: depopulation, adult bees with paralysis and/or deformed wings, mortality.
ID nr. 4009
Grilli G, Ferrazzi V, Cislaghi G, Sironi G, Lavazza° A, Gallazzi D
Pag. 112 di 300 31/08/2011 Parvovirus-like hepatitis in grey partridge (Perdix perdix): preliminary observations
Proceeding of the fifty-seventh Western Poultry Disease Conference : April 9-12, 2008 Puerto Vallarta,
Jalisco, Mexico / [s.l. : s.n., 2008]. - p 207-209. - 9 ref bib
Western Poultry Disease Conference (57th : Puerto Vallarta, Jalisco, Mexico : April 9-12, 2008)
During the summer 2006 a pathologic syndrome was observed in a rearing facilities of 3,000 partridges
(Perdix perdix) in Northern Italy. The disease was characterized by depression, anorexia, mild enteritis, and
sudden death in 50-day-old partridges. The mortality rate was very low (5%). Post-mortem examination
showed blood suffusions and catarrhal duodenitis. The liver was marbled. Spleen and bursa of Fabricius
frequently appeared atrophied. Other organs appeared norma]. Appropriate tissue samples were taken for
bacteriologic, histopathologic, and virologic examinations. Ali bacteriological cultures were negative for
specific pathogens. The negative staining electron microscopy examination of liver and spleen homogenates
evidenced the presence of both scattered and grouped small regular roundish particles about 20 nm in
diameter. Morphology and regular size of the particles suggested their identification as virions similar to
parvovirus
.
ID nr. 4010
Martella V, Lorusso E, Banyai K, Decaro N, Corrente M, Elia G, Cavalli A, Radogna A,
Costantini V, Saif LJ, Lavazza° A, Di_Trani L, Buonavolglia C
Identification of a porcine calicivirus related genetically to human sapoviruses
J Clin Microbiol. - Vol. 46 no 6 ( 2008). - p 1907-1913. - 40 bib ref
Impact factor: 3,708
First author Lavazza, Antonio
Whether animals may act as reservoirs for human caliciviruses is unclear. By sequence analysis of a short
fragment of the RNA-dependent RNA polymerase (RdRp) region, porcine sapovirus (SaV) strains that
genetically resemble human SaVs have been detected in piglets, but more-informative sequences (capsid
gene) were not available for a precise characterization. In this study, theY terminus (the Y end of open
reading frame 1 [ORF1I, including tbc polymerase complex and the complete capsid; ORF2; and tbc Y
untranslated region) of one such human SaV-like strain, 43/06-18p3/2006/It, was determined, revealing that
these viruses are more related genetically to human (47.4 to 54.9% amino acid identity) than to animal (35.2
to 44.7% amino acid identity) SaVs in tbc capsid gene. In addition, tbc recombination-prone RdRp-capsid
junction region was highly conserved with those of human SaVs of genogroup GI. The presence of porcine
viruses similar to human SaVs is a significant finding because of the potential for zoonotic infections or
generation of porcine/human recombinants.
ID nr. 4011
Quaglio F, Galuppi R, Marcer F, Morolli C, Bonoli C, Tampieri MP, Bassi° S , Gianaroli M,
Malagoli F
Episodes of mortality in white-clawed crafish (Austropotamobius pallipes complex) in three streams
of Modena Province (Northern Italy)
17th Simposium International Association of Astacology (IAA) : August 4-8, 2008, Kuopio, Finland / [s.l. :
s.n., 2008]. Simposium International Association of Astacology (17. : Kuopio, Finland : August 4-8, 2008)
A massive and apparently total mortality of white-clawed crayfish (Austropotamobius pallipes complex)
occurred in three streams in Modena province. Dead or moribund crayfish were collected and submitted to
mycological, parasitological, bacteriological, virological, and histopathological analyses. Extended Biotic Index
was evaluated for each water course and chemical-physical and bacteriological water analyses were carried
Pag. 113 di 300 31/08/2011 out. Saprophytes, ectosymbionts and ectocommensal organisms were frequently observed in crayfish. No
virus was found.. All water courses showed environmental pollution. A prolonged exposure to toxic
substances in the water could have exhausted the defense mechanisms so that the crayfish were more
susceptible to the action of opportunistic pathogenic agents and subsequently succumbed.
ID nr. 4012
Martella V, Bànyai K, Lorusso E, Bellacicco AL, Decaro N, Mari V, Saif L, Costantini V,
De_Grazia S, Pezzotti G, Lavazza° A, Buonavoglia C
Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets
Virus Gen. - Vol. 36 ( 2008). - p 365-373. - 43 bib ref
Impact factor: 1,362
Enteric caliciviruses (noroviruses and sapoviruses) are responsible for the majority of non-bacterial
gastroenteritis in humans of all age groups. Analysis of the polymerase and capsid genes has provided
evidence for a huge genetic diversity, but the understanding of their ecology is limited. In this study, we
investigated the presence of porcine enteric caliciviruses in the faeces of piglets with diarrhoea. A total of 209
samples from 118 herds were analysed and calicivirus RNA was detected by RT-PCR in 68 sample (32.59)
and in 46 herds (38.9%), alone or in mixed infection with group A and C rotaviruses. Sequence and
phylogenetic analysis of the calicivirus-positive samples characterized the majority as genogroup III (GGIII)
sapoviruses. Unclassified caliciviruses, distantly related to the representatives of the other sapovirus
genogroups, were identified in five herds, while one outbreak was associated with a porcine sapovirus related
genetically to human GGII and GGIV sapovirus strains. By converse, norovirus strains were not detected.
Altogether, these data suggest the epidemiological relevance of porcine enteric caliciviruses and suggest a
role in the etiology of piglets diarrhoea.
ID nr. 4015
Capucci° L, Lavazza° A
Rabbit haemorrhagic disease
Manual of diagnostic tests and vaccines for terrestrial animals : (mammals, birds and bees) / Office
International des Epizooties (OIE). - Paris : OIE, c2008. - p 947-961. - 41 bib ref
First author Capucci, Lorenzo
Rabbit haemorrhagic disease (RHD) is a highly contagious and acute fatal disease of the European rabbit
(Oryctolagus cuniculus), caused by a calicivirus. A similar disease, caused by a different calicivirus and
termed European brown hare syndrome (EBHS), has been described in the hare (Lepus europaeus). The
aetiological agent is a different calicivirus, antigenically related to the RHD virus (RHDV). RHD is
characterised by high morbidity and high mortality (40-90%), and spreads very rapidly by direct and indirect
transmission. Infection can occur by nasal, conjunctival or oral routes. Transmission of RHD is facilitated by
the high stability of the virus in the environment. The incubation period varies from 1 to 3 days, and death
usually occurs 12-36 hours alter the onset of fever The clinical manifestations have been described mainly in
the acute infection (nervous and respiratory signs, apathy and anorexia). Clear and specific lesions, both
gross and microscopic, are present. There is primary liver necrosis and a massive disseminated intravascular
coagulopathy in all organs and tissues. The most severe lesions are in the liver, trachea and lungs. Petechiae
are evident in almost all organs and are accompanied by poor blood coagulation. Identification of the agent:
The liver contains the highest viral titre and is the most suitable organ for viral identification. As no satisfactory
growth conditions or sensitive cell substrates have been established, in-vitro isolation cannot be employed.
The haemagglutination test using human Group O red blood cells was the first test applied for routine
laboratory diagnosis of RHD. However, other tests (negative-staining electron microscopy, sandwich enzymelinked immunosorbent assay [ELISA], immunohistological staining, polymerase chain reaction and Western
blot) have shown a higher level of specificity and sensitivity. Serological tests: Characterisation and titration of
specific antibodies arising from natural Infection or from immunisation are performed using the
haemagglutination inhibition test or an indirect or competitive ELISA. The following reagents are prepared:
Pag. 114 di 300 31/08/2011 antigen from infected rabbit liver, anti-RHDV serum from convalescent or hyperimmunised rabbits, and
negative serum from rabbits fully sensitive to RHDV Infection. Monoclonal antibodies have been produced in
several laboratories. Some laboratories have produced a recombinant antigen, VP60 structural protein
expressed in baculovirus, which can also be used as a diagnostic reagent. Requirements for vaccines and
diagnostic biologicals: Indirect control of the disease is achieved by vaccination using a killed vaccine
prepared from clarified liver suspensions of experimentally infected rabbits and subsequently inactivated and
adjuvanted. Vaccinated animals quickly produce solid protective immunity against RHDV Infection (within 5-10
days) and experimental data indicate that protection lasts for a long period (over 1 year).
ID nr. 4016
Stacchiotti A, Morandini F, Bettoni F, Rodella LF, Grigolato P, Lavazza° A, Aleo MF
Nephrotoxicity induced by inorganic Hg(II) and Pb(II): a microscopic and biochemical in vitro study
14th European Microscopy Congress : Aachen, 1-5 September 2008 / a cura di Ankje Aretz, Benita
Hermanns-Sachweh, Joachim Mayer. - [s.l : s.n, 2008]. - v. 3: Life science. - p 289-290. - 4 bib ref
European Microscopy Congress (14th : Aachen, Germany : 1-5 September 2008)
First author Lavazza, Antonio
A close link between stress protein up-regulation and oxidative damage may provide a novel therapeutic tool
to counteract nephrotoxicity induced by toxic metals in the human population, mainly in children, of
industrialized countries. Here we analysed the time course of the expression of several heat shock proteins,
glucose-regulated proteins and metallothioneins in a rat proximal tubular cell line (NRK-52E) exposed to
subcytotoxic doses of inorganic mercury and lead. Concomitantly, we used morphological and biochemical
methods to evaluate metal-induced cytotoxicity and oxidative damage.
ID nr. 4017
Stacchiotti A, Pedretti N, Aleo Mt, Lavazza° A
Effects of schisandrin B on NRK52E cells exposed to inorganic mercury
Ital J Anat Embryol. - Vol. 113 n 2 Suppl 1 ( 2008). - p 266. - 3 bib ref
Congresso Società Italiana di Anatomia e Istologia Meeting of the Italian Society of Anatomy and Histology
(62. : Verona, Italia : 14-16 September 2008)
Aims: The exposure to mercuric chloride (HgCl2) causes an acute oxidative injury in renal tubular epithelial
cells [1]. Schisandrin B (Sch.B) is a dibenzocycloocta diEne derivative from the fruit of Schisandra chinensis,
a popular Chinese herb, successfully used against viral and toxic hepatitis and cardiac ischemic damage [2].
Stress proteins (Hsps) are cytoprotective chaperones enhanced in the kidney durin, mercury toxicity [3]. Since
the renal efficacy of Sch.B is largely unknown, this in vitro study is aimed to better clarify its role against
HgCl2-nephrotoxicity on a rat proximal tubular cell line. Wc focused on morphology and on the expression of
two Hsps involved in oxidative damage, constitutive HSP25 and inducible HSP72. Methods: Sch.B dissolved
in DMSO was added to medium up to 10[alpha]M concentration. NRK-52E cells in grrowin phase were
incubated for 48h with 10[alpha]M Sch.B or DMSQ-alone before 24h treatment with 20(alpha)M HgCl2.
Ultrastructural analysis was performed on different treatments. Both presence and abundance of Hsps were
tested by immunohistochemistry and innnunoblotting using specific polycIonal and monoclonal antibodies.
Results and Conclusions: Sch.B-alone treated NRK52E cells have shown normal ultrastructure. Alter Sch.B
pretreatment, reduced necrosis and more preserved mitochondria were observed respect to HgCl2 exposed
cells. We did not find any difference in Hsps expression between DMSQ and Sch.B-alone treated cells.
Indeed llSP72 was undetectable and HSP25 was moderatly expressid. However, after exposure to
20(alpha)M HgCl2 for 24h, both Hsps enhanced. Remarkably, after Sch.B and mercury coadministration,
HSP72 persisted while HSP25 increased further. These novel in vitro data suggest that Sch.B mitigates
mercury-induced damage in NRK52E cells by maintenance of specific Hsps.
Pag. 115 di 300 31/08/2011 ID nr. 4018
Dusi° G, Bozzoni° E, Assini° W, Tognoli° N, Gasparini° M, Ferretti° E
Confirmatory method for the determination of resorcylic acid lactones in urine sample using
immunoaffinity cleanup and liquid chromatography-tandem mass spectrometry
Anal Chim Acta. - Vol. 637 ( 2009). - p 47-54 - 18 bib ref
Impact factor: 3,146
First author Dusi, Guglielmo
The presence of Zeranol (a-zearalanol) in urine samples due to natural contaminantion or illegal treatment is
under debate within the European Union. The simultaneous determination of zeranol, its epimer taleranol (ßzearalanol), zearalanone and the structurally-related mycotoxin zearalenone with the corresponding a- and ßzearalenol metabolites appears to be critical in deciding whether an illegal use has occurred. The aim of this
study is to develop and validate a simple analytical procedure applicable to bovine and swine urine samples
for the determination of all six resorcylic acid lactones. After an enzymatic deconjugation, the urine was
subjected to a one-step cleanup on a commercially available immunoaffinity chromatography cartridge. The
analytes were detected by liquid chromatography-negative-ion electrospray tandem mass spectrometry using
deuterium-labelled internal standards. The method was validated as a quantitative confirmatory method
according to European Commission Decision 2002/657/EC. The evaluated parameters were: linearity,
specificity, precision (repeatability and intra-laboratory reproducibility), recovery, decision limit, detection
capability and ruggedness. The decision limits (CCa) obtained, were between 0.56 and 0.68 µg L-1; recovery
above 66 % for all the analytes. Repeatability was between 1.4 % and 5.3 % and within-laboratory
reproducibility between 1.9 % and 16.1 % for the six resorcylic acid lactones.
ID nr. 4019
Magistrali C, Facinelli B, Princivalli MS, Barocci S, Merialdi° S, Ciuti F, Magi G, Palmieri C
Analisis of genetic diversity and virulence-associated gene profiling of Streptococcus isolates from
symptomatic and asyntomatic pigs in Italy
20th IPVS Internationl Pig Veterinary Society Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - V. 2. - cdrom p 338. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, South Africa : 22-26 June 2008)
not found.
ID nr. 4020
Merialdi° G
Escherichia coli associated diseases in post-weaned piglets
Obiet Docum Vet. - Vol. 29 no 2 ( 2008). - p 17-22. - 33 bib ref
First author Merialdi, Giuseppe
The most important enteric and systemic diseases caused by E. coli infection in weaned piglets are reviewed
focusing on predisposing factors as alimentary changes at weaning and their effect on intestine physiology
and microflora.
ID nr. 4021
Macini P, Squintani G, Finarelli AC, Angelini P, Martini E, Tamba° M, Dottori° M, Bellini R,
Santi A, Loli_Piccolomini L, Po C
Pag. 116 di 300 31/08/2011 Detection of West Nile virus infection in horses
EuroSurveillance http://www.eurosurveillance.org/viewarticle.aspx?articleID=18990 (ultimo accesso
07/05/2009). - Vol. 13 no 39 ( 2008). - p 1-2. - 1 bib ref
not found.
ID nr. 4022
Bano L, Merialdi° G, Bonilauri° P, Dall'Anese G, Capello K, Comin D, Cattoli G,
Sanguinetti V, Hampson DJ, Agnoletti F
Prevalence, disease associations and risk factors for colonization with intestinal. spirochaetes
(Brachyspira spp.) in flocks of laying hens in north-eastern Italy
Avian Pathol. - Vol. 37 no 3 ( 2008). - p 281-286. - 32 bib ref
Impact factor: 1,257
First author Merialdi, Giuseppe
The present study investigated the occurrence of anaerobic intestinal spirochaetes of the genus Brachyspira
in laying hen flocks in Treviso province, north-eastern Italy, with respect to prevalence, spirochaete species
present, disease associations and risk factors for colonization. A total of 450 faecal samples from 45 sheds on
29 laying hen farms were cultured for intestinal spirochaetes. Nineteen sheds on 12 farms contained chickens
with symptoms consistent with avian intestinal spirochaetosis, including reduced egg production, wet litter
and/or pasty vents. Spirochaetes were isolated from 157 (34.8%) samples from 21 (72.4%) farms, and from
32 (71.1%) sheds. From these positive samples, 189 spirochaetal isolates were speciated using three
polymerase chain reaction assays and a restriction fragment polymorphism analysis of 16S rDNA polymerase
chain reaction products. Overall, 52 (27.5%) isolates were identified as pathogenic Brachyspira intermedia, 26
(13.8%) as pathogenic Brachyspira pilosicoli, 93 (49.7%) as non-pathogenic (Brachyspira
innocenslBrachyspira murdochii), and 18 (9.6%) were unidentified. Faeces from 14 sheds (31%) on 10 farms
(34.5%) contained B. intermedia and/or B. pilosicoli, and disease consistent with avian intestinal
spirochaetosis was observed in nine of these sheds on seven farms. There was a significant association (P
=0.042) between the presence of spirochaetes and using deep pits rather than conveyor belts for manure
disposal. Sheds housing chickens >40 weeks of age were significantly more likely to contain spirochaetes (P
=0.048) and pathogenic species (P =007) than sheds housing younger chickens. A significant association
(P=0.02) was found between infection with pathogenic spirochaetes and reduced egg production..
ID nr. 4023
Milandri A, Cangini M, Costa A, Giacobbe MG, Poletti R, Pompei M, Riccardi E, Rubini° S,
Virgilio S, Pigozzi S
PSP toxic profile of mussels collected in various marine areas of Italy
Biol Mar Mediterr. - Vol. 15 no 1 ( 2008). - p 38-41
During the period November 2006 - August 2007, PSP toxins bave been detected in mussels from three
different Italian marine areas. Here we report about the toxic profîles of these molluscs, as revealed by HPLCFL. Differences arnong the three areas are presumably related lo the presence in the presence in the
Alexandrium (Dinophyceae) species: A. catenella in Sardinia and A. minutum in Sicily and Emilia Romagna.
ID nr. 4024
Caprai° E, Accurso° D, Galletti° G, Licata E, Carone° V
Aflatoxin M1 contamination in bovine milk : results during five years of surveillance in Emilia
Romagna region
Pag. 117 di 300 31/08/2011 Sci Tec Latt Casearia. - Vol. 59 no 5 ( 2008). - p 379-394. - 7 bib ref
First author Caprai, Elisabetta
Owing to proliferation of aflatoxin B1 in feed during the 2003 and the consequent increase of aflatoxin M1
concentration in bovine milk, the Veterinary Service of Emilia Romagna region started a particular plane to
monitor the depletion of these residues in bovine milk, to preserve public health. The monitoring plane carried
out from 2004 consisted on a monthly sampling in 300 bovine farms. According to the results obtained, the
number of samples analysed has been reduced during the years (100 samples in 2007) keeping a bovine
farm monitoring anyway. During the 2004 the average concentration of aflatoxin M1 in the samples analysed
has been reduced from 0.027 pg/kg to 0.022 pg/kg, thanks to preventive measures actuated owing to the
emergency. The change of main aflatoxin M1 concentration from 2005 to the beginning of 2008 was the same
of the 2004, but with an average value fixed around 0.010 pg/kg. These concentrations movements showed
however an annual increase between August and November; it would be necessary to respect the number
and the frequency of monthly sampling in the farms and dairy, as the regional plane required and eventually to
increase the controls attendant in the critical period (the beginning of autumn).
ID nr. 4025
Pongolini° S, Bergamini° F, Iori° A, Migliore S, Corradi A, Bassi° S
Prion protein genotypes of Italian sheep breeds with lysine-171 and phenylalanine-141 detection
Vet Microbiol. - Vol. 137 ( 2009). - p 18-23. - 25 bib ref
Impact factor: 2,370
Project PRC2003011 - Genotyping of sheep in Emilia-Romagna Region for Scrapie resistance. - Pongolini
Dr. Stefano - Solo - MINSAN - 2003 - 35000,00 EUR - 26/04/2004 0.00.00 - 25/04/2005 0.00.00 1 - N4 - Modena - Pongolini Dr. Stefano - I
First author Pongolini, Stefano
Amino acid polymorphisms of the prion protein gene influence sheep susceptibility to classical and atypical
scrapie. Substitutions at codons 136, 154 and 171 play an important role in classical scrapie. Codon 141
leucine to phenylalanine mutation (AFRQ) has been recognized as an increased risk factor for atypical
scrapie. In addition a rare allele with lysine at codon 171 (ARK) has been detected in Mediterranean sheep
breeds. The presence of ARK poses two problems: the determination of its frequency and its possible
interference with genotyping output of routine methods lacking specific detection capacity for ARK. The aim of
our work was the development of a routine genotyping method with the capacity to identify ARK and AFRQ in
addition to the normally detected alleles and to determine the frequencies of all these alleles in 5 main Italian
breeds: Sarda (n = 2494), Bergamasca (n = 2686), Appenninica (n = 297), Comisana (n = 361) and Massese
(n = 402). A multiplex primer extension assay targeting the six single nucleotide polymorphisms of interest
was developed. Allele frequencies revealed a very low level of ARR in Bergamasca (6.91%) as opposed to
the other breeds, very diverse levels of AFRQ ranging from absence in Comisana to 10.70% in Massese and
a restricted presence of ARK. This allele has only been detected in Bargamasca with a significant 3.67% and
marginally in Appenninica (0.34%). These results underline the need for adequate routine methods for
genotyping of breeds with alleles that can interfere with typing of important codons such as the case of ARK
for codon 171.
ID nr. 4028
Pennelli D, Salogni° C, Tagliabue° S, Alborali° GL
Antibiotic resistance in Lactococcus garvieae strains isolated from salmonid fishes during the
period 1994-2005 : preliminary results
Ittiopatologia. - Vol. 5 ( 2008). - p 65-72. - 14 bib ref
In Italy Lactococcosis is considered the main liable for yearly shrinkage of trout production. Antibiotics are
Pag. 118 di 300 31/08/2011 used to control the disease, even if it is necessary to consider that a wrong employment moy cause a
development of antibiotic resistance Our aim is to provide a first share to in vitro Lactococcus garvieae
antimicrobial resistance study; we examined 73 strains isolated from rainbow trout (Oncorhynchus mykiss)
during the period 1994-2005, reared in farms placed in the north Italy. It has been applied the Kirby-Bauer
agar diffusion method; each strain was tested for 27 different antimicrobial drugs. All the strains were
flumequine (100%) resistant; high rates of drug resistance were detected for colistin, tiamulin (97,3916) and
sulfadiazine (96%). Full susceptibility (100%) was observed for amoxicillin, clavulanic acid + amoxicillin and
gentamicin.
ID nr. 4029
Salogni° C, Aquaro G, Galli P, Gelmetti° D, AlboralI° LG
Infection of Gyrodactylus turnbulli Harris, 1986 (Monogenea) on cultured guppies, Poecilia reticulata
in Italy
Ittiopatologia. - Vol. 5 ( 2008). - p 635-43. - 24 bib ref
First author Salogni, Cristian
Cutaneous monogenean gyrodactylid parasites are main pathogenic species speciftc factor. Pathogen effects
are due to lesions caused by adhesive complex armed with anchors and hooks (haptor). Moreover
gyrodactylid parasites present a high reproductive and diffusive capacity. This work describes a Gyrodactylus
turnbulli and Urocleidoides reticulatus infection in Italian breeding guppies, that occurred in the summer 2006
and that caused high mortality. Juvenile ftshes (from 2-3 days to one month) showed the higher mortality than
adult ftshes. Analyzed specimen showed feeding problems, swimming with erratic or swinging movements
and frequent rubbings against rigid surfaces. Thins, especia/1y the cauda/ one, became thinner to ftliform.
Evident lesions, white opaque spots, were homogeneously scattered on the skin of adult ftshes. In juvenile
ftshes, skin lesions were localized mainly on dorsal region and cauda/ peduncle. Lesions in adult ftshes seem
to be less severe than juvenile specimen. Pathogenicity was sub-acute in juvenile ftshes (mortality 80-100% in
1-2 weeks from the fast symptoms). Effect of infection in adults is chronic mortality. Parasitological,
microscopica!, cultural, mycological, virological and histological routinary analysis were carried out on
samples. All the examined samples showed a massive skin infection by G. tumbulli, moreover gills presented
a low intenity infection by U. reticulatus. Histological analysis revealed that, in some specimen, skin parasites
were associated with a limpholeucocytic myositis. Bacteriological, mycological and virological analysis
resulted negative.
ID nr. 4030
Salogni° C, Cervellione F, Zanoni° M, Gelmetti° D, Alborali° GL
A severe outbreak of mycobacteriosis due to Mycobacterium marinum in farmed rainbow trout
(Oncorhynchus mykiss) in Italy
Atti del XV convegno nazionale della società italiana di patologia ittica : 22-24 Ottobre 2008, Erice (TP) / [s.l.
: Società italiana di patologia ittica, 2008]. - p 29
Convegno nazionale della Società italiana di patologia ittica (15. : Erice (TP) : 22-24 Ottobre 2008)
First author Salogni, Cristian
A low mortality rate was continuously observed in a rainbow trout (Onorhynchus mykiss) farm located in the
north of Italy during summer 2007, starting 21 days post intraperitoneal vaccination against Lattococcus
garviae. During the previous months fish were affected by proliferative kidney disease (PKD). The cumulative
mortality during summer 2007 peaked at 95 % of the population, affecting approximately 10 tons. All fish
presented melanosis, exophthalmia, anorexia and lethargia. Necroscopy revealed the presence of greyish
nodular lesions localized into spleen, liver and kidney; histology showed granulomatous lesions throughout
the viscera with the presence of acid fast bacteria. Mycobacterium marinum has been isolated and identified
by biochemistry assay, polymerase chain reaction (PCR) and sequencing rRNA 16s..
Pag. 119 di 300 31/08/2011 ID nr. 4031
Salogni° C, Mioso° PM, Astuti M, Domenechini M, Alborali° Gl
Monitoring plane on Opistorchiasis in freshwater fishes of Lombardia (Italy)
Atti del XV convegno nazionale della società italiana di patologia ittica : 22-24 Ottobre 2008, Erice (TP) / [s.l.
: Società italiana di patologia ittica, 2008]. - p 42
Convegno nazionale della Società italiana di patologia ittica (15. : Erice (TP) : 22-24 Ottobre 2008)
First author Salogni, Cristian
The aim of this study, made by Brescia State Diagnostic Laboratory of Ichthyopathology (IZSLER), is to
monitoring the presence of Opisthorchis sp. in the freshwater fishes of Northern Italy. In human
opisthorchiasis is connected to consumption of raw infested fresh-water fishes belong Cyprinidae family. In
man this infestation is characterized by hepatic location of the parasite, responsible for catarrhal cholangitis
than can evolves in fibrosis but also in tumor. We have tested 318 fishes (in prevalence Cyprinid fishes) from
the most important Northern lakes (Garda, Iseo, Idro, Como, Maggiore, Pusiano, Varese, Garlate e Lugano).
The analyses executed include: transillumination on each fish’s fillet and chloro-petic digestion of fillets' pools.
We found 34 samples positive for parassitic cysts in muscle: crucian carp –Carassius carassius(pos.2/36tot.), tench –Tinca tinca-(11/42), rudd -Scardinius erythrophtalmus- (12/70), carp – Cyprinus carpio(2/9), varione –Leuciscus souffia- (2/12), european chub –Squalius cephalus- (2/10) and also 3/5 positives for
catfish –Ictalurus melas- from Garlate lake.
The identification of the isolated metacercarie has been performed on the morphological base and allowed the
classification of the parasities similar to Digenean Trematodae of Opisthorchidae family. Further indispensable
investigations with PCR systems are necessary for a certain identifications of trematode larvae isolated.
ID nr. 4032
Salogni° C, Zanoni° M, Tagliabue° S, Pacciarini° Ml, Giovannini° S, Alborali° Gl
Isolation of Mycobacterium marinum from granulomatous lesions in restocking carps (Cyprinus
carpio)
Atti del XV convegno nazionale della società italiana di patologia ittica : 22-24 Ottobre 2008, Erice (TP) / [s.l.
: Società italiana di patologia ittica, 2008]. - p 30
Convegno nazionale della Società italiana di patologia ittica (15. : Erice (TP) : 22-24 Ottobre 2008)
First author Salogni, Cristian
This survey describes the occurrence of granulomatous lesions caused by Mycobacterium marinum in carps
(Cyprinus carpio), which are intended for the introduction in North Italy water. Some carps which were found
dead in the transport tank during the shipping were analyzed in laboratory. Gross lesions were: skin ulcera
and greyish nodular lesions, whose size varying from few millimetres to half a centimetre, predominantly
disseminated in visceral organs as liver, spleen and kidney. Histological exams of the organs with lesions
were carried out. The differential diagnosis was carried out between the diseases able to cause
granulomatous and/or nodular lesions such as systemic mycosis, parasitic infection (Acanthamoeba sp.,
Hoferellus sp., Sphaerospora sp., etc.), mycobacteriosis and not-infectious diseases like nephrocalcinosis and
neoplasia. Parasitological, bacteriological, mycological and virological exams have been executed. Pooled
organs (liver, spleen and kidney) had been analysed to detect mycobacteria on specific egg yolk medium
(Löwenstein-Jensen and Stonebrink). M. marinum had been isolated and identified by biochemistry assay,
PCR and rRNA 16S sequencing. The histological exams found granulomatous lesions and bacteria positive to
Ziehl-Neelsen stain. The episode described represents a warning of the presence of M. marinum in the
national territory in a fish species, the carp, that is important for the food production and the restocking..
ID nr. 4033
Pag. 120 di 300 31/08/2011 Merialdi° G, Barigazzi° G, Bonilauri° P, Tittarelli° C, Bonci° M, D'incau° M, Dottori° M
Longitudinal study of salmonella infection in italian farrow-to-finish swine herds
Zoonoses Public Health. - Vol. 55 ( 2008). - p 222-226. - 16 bib ref
Impact factor: 1,478
Project PRC2004005 - Development of a risk assessment model relating to Salmonella contamination of
Italian heavy-swine carcasses - Merialdi Dr. Giuseppe - Solo - MINSAN - 2004 - 55000,00 EUR 01/07/2005 0.00.00 - 30/06/2007 0.00.00 - 1 - N8 - Reggio Emilia - Dottori Dr. Michele - I
2 - N0 - CEREV - Tamba Dr. Marco - I
3 - N5 - Parma - Barigazzi Dr. Giuseppe - I
4 - N4 - Modena - Bassi Dr. Stefano - I
5 - N7 - Ravenna (Lugo di Romagna) - Marzadori Dr. Fausto - I
First author Merialdi, Giuseppe
A longitudinal study of Salmonella enterica infection was carried out in five
Italian farrow-to-finish swine herds previously known to be infected by Salmonella.
Five litters were randomly selected from each herd and in each litter six
piglets were randomly selected and individually identified. Thus, the study
included 30 pigs from each farm. At weaning, individual blood samples were
collected for serological examination from all selected piglets and on the lame
day from all sows in the farrowing unit. Piglets were bled again at approximately
60, 90, 150, 210 and 270 days of life whereas the last blood sample was
collected at slaughtering. In one of the herds, in which the duration of productive
cycle was about 12 months, the last blood samples were collected at
350 days of life. With the same time scheduling, five pen pooled faecal samples
were collected from each herd for bacteriological examination. At slaughtering,
mesenteric lymph nodes were collected from each ear-tagged pig. Seroprevalente(cot off S/P ratio 0.25) in
sows varied from 93.8% to 100%. In Tourherds, sero-prevalente in piglets showed a similar profile with
complete declineof maternal antibodies at day 60 and elear sero-conversion between day 90 andday 150. In
one herd, sero-conversion was observed earlier and 56% of piglets were positive at day 90. The peak of seroprevalente was observed between day 210 and day 270. Sero-prevalente at slaughtering varied from 66% to
100%. Salmonella was isolated from faecal samples in four of five herds. No Salmo nella was isolated from
mesenteric lymph nodes at slaughter in two of the herds. Culture prevalente from mesenteric lymph nodes in
the other three herds ranged from 3.3% to 30%. This longitudinal study provides original information about
epidemiological dynamics of Salmonella enterica infection in Italian swine herds in consideration of the unique
extended fattening period typical of the Italian production.
ID nr. 4034
Ferrari° M, Villa° , De_Benedictis° P, Toffan A, Dotti° S
Use of cell cultures for avian Influenza virus isolation and cultivation
Atti Soc Ital Sci Vet. - Vol. 62 ( 2008). - p 127. - 3 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (62 : S. Benedetto del
Tronto (AN) : 2008)
First author Ferrari, Maura
The risk of avian Influenza virus transmission to human with the emergente of a novel pandemic is of high
concern. In order to overcome the limits presented by embryonated eggs (EE), cell cultures may represent a
convenient biological system. The aim of this study was to compare the susceptibility of different cell lines
(NSK, MDCK UMNSAH/DF1) in comparison to embryonated eggs, to avian Influenza virus (AIV) of high and
low pathogenicity. 3 Groups of 30-40 day-old SPF chickens were experimentally infected with H5N2, H7N 1
and H7N3 subtype. The results of the study have shown that RT-Real Time PCR can detect viral RNA in
almost all the pathological samples. Among the biological system, EE is confirmed to be the most susceptible
while in the cell lines virus isolation has been performed only in NSK and MDCK. No virus was detected in
UMNSAH/DFI cells.
Pag. 121 di 300 31/08/2011 ID nr. 4036
Quaglio F, Galupi R, Marcer F, Morolli C, Bonoli C, Fioretto B, Tampieri MP, Bassi° S,
Lavazza° A, Gianaroli M, Malagoli F
Mortality episodes of white-clawed crafish (Austropotamobius pallipes complex) in three streams of
Modena province (Northern Italy)
Ittiopatologia. - Vol. 5 ( 2008). - p 99-127. - 103 bib ref
A massive and apparently total mortality of white-clawed crayfish (Austropotamobius pallipes complex)
occurred in Rossenna (09/04), Lerna (02/05) and Selve (06/05) streams in Modena province, Northern Italy. In
all three streams the deceased crayfish were in different states of preservation and only recently dead or
moribund crayfish were collected and submitted to mycological, parasitological, bacteriological, virological,
and histopathological analyses. Extended Biotic Index was evaluated for each water course and chemicalphysical and bacteriological water analyses were carried out. Saprophytes, ectosymbionts and
ectocommensal organisms
were frequently observed in crayfish. No virus was found. Fusarium sp. was the most frequently isolated
mycete and it occurred in pure culture from the specimens coming from Selve creek. Furthermore Mucor sp.
and Penicillium sp. were detected on the crayfish from the river Rossenna, while Trichoderma sp., Alternaria
sp., Gliocladium sp. and Mucor sp. from Lerna specimens. The bacteriological investigation recorded Hafnia
alvei from both Lerna stream and Selve creek samples, while Aeromonas hydrophila only from Lerna. During
the histological investigation, all crayfish showed severe fungal hyphae infiltrations from the exoskeleton
through the superficial skeletal to the muscle hypodermis. All water courses showed environmental pollution.
A prolonged exposure to toxic substances in the water could have exhausted the defense mechanisms so that
the crayfish were more susceptible to the action of opportunistic pathogenic agents and subsequently
succumbed.
ID nr. 4037
Pongolini° S, Bergamini° F, Bassi° S
A new genotyping strategy for efficient scoring of closely positioned SNPs in the ovine prion protein
gene
Mol Cell Probes. - Vol. 23 ( 2009). - p 122-125. - 16 bib ref
Impact factor: 2,196
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Pongolini, Stefano
Amino-acid polymorphisms of the ovine prion protein have been known to influence susceptibility to scrapie
for many years. Recently, a role in both classical and atypical scrapie was assigned to new mutations,
increasing the overall number of polymorphisms of interest for breeding plans. Besides, the high number and
density of polymorphisms in the prion protein gene (PrP) and the presence of unusual mutations in some
breeds hampers genotyping methods, making multiplexing difficult and sometimes compromising analytical
results. We developed a multiplex genotyping method for the ovine PrP that overcomes the limitations posed
by the high number and density of the polymorphisms to interrogate. Nine primers were designed to be
compatible in a single primer-extension reaction developed for routine genotyping, with the capacity to identify
Pag. 122 di 300 31/08/2011 the following polymorphisms: A136V, M137T, 1-141F, I142K, R154H, Q171R, Q171H, Q171K and N176K.
Site-specific mutations were inserted in primer sequences in order to prevent extension of reciprocally
complementary primers. Complete accuracy and repeatability of the assay was assessed with reference to 97
sequenced samples.
The presented method constitutes an improved tool for ovine PrP genotyping and a general strategy for the
use of primer extension in a genetic context of high density of polymorphisms..
ID nr. 4038
Gamba° V, Terzano C, Fioroni L, Moretti S, Dusi° G, Galarini R
Development and validation of a confirmatory method for the determination of sulphonamides in
milk by liquid chromatography with diode array detection
Anal Chim Acta. - Vol. 637 ( 2009). - p 18-23 - 35 bib ref
Impact factor: 3,146
First author Gamba, Valentina
A simple and rapid multiresidue method for the determination of seven sulphonamides residues (sulfadiazine,
sulfapyridine, sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine and sulfaquinoxaline) in
milk samples was developed and validated. The drugs were extracted with a mixture chloroform/acetone and
simply cleaned up on a cation exchange solid phase extraction column. The analytes determination was
carried out using liquid chromatography with diode array detection (DAD). The procedure has validated as a
quantitative confirmatory method according to the European Union (EU) Decision 2002/657/EC. The
developed method shows good linearity, specificity, precision (repeatability and intra-laboratory
reproducibility), ruggedness and is able to confirm each sulphonamide residue above 30 µg kg-1. Decision
limits (CCa) around 110 µg kg-1 and recovery above 56% were obtained for all the analytes. The results of
the validation process demonstrate that the method is suitable for application, as confirmatory method, in
European Union statutory veterinary drug residue surveillance programmes. In addition, a hypothetical
situation of sample judgement (compliance or not) in the case in which, at the saure time, two different
sulphonamides are found, is discussed.
ID nr. 4039
Candotti° P, Rota_Nodari° S
A case report of blue spots and abortion syndrome in northern Italy
20th IPVS Internationl Pig Veterinary Society Congress : Durban, South Africa, 22-26 June 2008 :
proceedings / [s.l : s.n, c2008]. - v. 2. - cdrom p 559. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (20th : Durban, South Africa : 22-26 June 2008)
First author Candotti, Paolo
not found.
ID nr. 4040
Ferrari° M, Renzi° S, Villa° R, Lombardi° TG, Sesso L
Quality assurance and quality control programmes on human and animal cell cultures
ISBER 2009 Annual Meeting & Exhibits : ISBER, Celebrating a Decade of Growth and Development in
International Biorepository Excellence : May 12 - 15, 2009 Portland, Oregon, USA / [s.l. : s.n., 2009]. - p 96
ISBER 2009 Annual Meeting & Exhibits : Portland, Oregon, USA : May 12 - 15, 2009)
First author Ferrari, Maura
Pag. 123 di 300 31/08/2011 Animal and human cells are increasingly used in basic research, large-scale production of biologicals and,
more recently, for therapeutic purposes. In order to ensure the reproducibility, reliability, traceability, safety,
and proper application of any result or product obtained from cells, it is necessary to maintain high standards
of laboratory practices. In our cell culture bank all quality assurance procedures are carried out with the
objective to control] generic parameters Iike viability and safety. Furthermore, microbial, endogenous and
exogenous virus contaminations are evaluated by using standard operating procedures (SOPs). Bovinederived biologicals must comply with the requirements laid down in the note for guidance (EMEA/410102 Rev.
1-May 2001). Finally, the identity of each cell type is also evaluated. Moreover, routine environmental
monitoring of the laboratory apparatus is carried out at pre-determined intervals. Ali continuous cell lines are
obtained from reliable sources. Also, master and working cell batches are routinely established. Besides
continuous and primary cell cultures a mesenchymal stem cell bank, with samples of animal origin (rats,
sheep. and horses), has been established as well. Ali of them are monitored by the aforementioned validated
and sensitive diagnostic tests, for quality and absence of contamination in order to enforce biosafety. To
conclude, reliable and reproducible cells can be provided if a bank is accredited by an appropriate competent
authority and works in accordance to the principles of Good Laboratory Practices (GLP) and control)
procedures laid down in the International Guidelines (European Pharmacopoeia)..
ID nr. 4041
Ferrari° M, Villa° R, Dotti° S, Losi° CG
The italian cell culture reference centre
ISBER 2008 Annual Meeting & Exhibits : Global biobanking collaborations : challenges and opportunities :
May 18 - 21, 2008, Bethesda, Maryland, USA / [s.l. : s.n., 2008]. - p 39
ISBER 2008 Annual Meeting & Exhibits : Bethesda, Maryland, USA : May 18 - 21, 2008)
Project relopianoregionalesa - Regional Blood Plan 2006 “Adult staminal cells transplantation for cellular
replacement-therapy purposes” - Prof.Franco Locatelli - UO - REGIONE - 2006 - 1230000,00 EUR
- 10/08/2006 0.00.00 - 09/08/2008 0.00.00 - 8 - L1 - Reparto Substrati Cellulari - Ferrari Dr.ssa
Maura - I
First author Ferrari, Maura
The National Cell Culture Reference Laboratory, located at the Institute Zooprofilattico in Brescia, collects a
large amount of cell cultures belonging to several animal species, for a total of about 35000 frozen vials. In
particular, it includes 424 established cell lines which have been obtained either from normal or from cancer
tissues, 54 hybridomas, 57 primary cell culture types from various organs and tissues and recently, also
biological) samples from patients with Alzheimer's disease have been collected. The Cell Culture Centre
laboratory in Brescia is divided into the following main sectors: general services, culture media preparation,
manipulation of established and primary cell lines, quality control) (in a separate area from the previous one),
liquid nitrogen cell storage. Together with improvements in preparation methods of new cell types, particular
attention is paid to quality control) in order to distribute well characterizes products free from contaminants. Ali
tests (detection of bacteria, mycoplasma, human and animal viruses, bacterial endotoxins, cell line cross
contaminations, tumorigenity "in vitro" and "in vivo") are performed in accordance with European
Pharmacopeia, and are carried out on final products. In addition to cell cultures, a large number of viruses
from several animal species (pig, cattle, fowl, horses, sheep/goat) and human, each grown in the appropriate
cell culture system, are banked. Moreover, immune sera prepared in laboratory animals specific for each virus
have been prepared and collected. The biological materials are supplied as reference samples for either "in
vivo" tests and "in vitro" virological and molecular biology investigations.
ID nr. 4042
Losi° CG, Sesso° L, Ferrari° M,
Isolation, characterization and storage of animal mesenchymal stem cells
ISBER 2008 Annual Meeting & Exhibits : Global biobanking collaborations : challenges and opportunities :
Pag. 124 di 300 31/08/2011 May 18 - 21, 2008, Bethesda, Maryland, USA / [s.l. : s.n., 2008]. - p 38
ISBER 2008 Annual Meeting & Exhibits : Bethesda, Maryland, USA : May 18 - 21, 2008)
First author Losi, Claretta_Gioia
Mesenchymal stem cells (MSCs) are multipotent cells resident in a several adult tissues (as bone marrow and
fat) and in umbilical cord, that are able to differentiate along multiple lineages such as chondrocytes,
osteoblasts and adipocytes. This plasticity suggests their potential in reparative medicine and in tissue
engineering, a powerful alternative to organ and tissue transplantation based on the use o differentiated cells.
In veterinary field, MSC therapy is mainly applied in horses and dogs to treat tender ligament, bone and
cartilage injuries. Furthermore, it has been shown that MSC are characterised by an immunosuppressive
action that permits the use of allogenic cell transplants allowing for the creation of stem cell banks for
heterologous implants. The National Cell Culture Reference Laboratory, located at the Istituto Zooprofilattico
in Brescia, is already involved in biobanking of numerous cell culture types. The experience in stem cell
technology, previously restricted to research on animal models, can also be potentially used for therapeutic
approaches in horses and dogs. On the basis of these characteristics, Cell Culture Laboratory would
represent a potential Centre in Italy for animal mesenchymal stem celi isolation, characterization and storage.
ID nr. 4043
Ferretti° E, Castrezzati° G, Gasparini° M
Matrix effect in the determination of organochlorine pesticide resisues in fat
7th Euro Pesticide Residues Workshop : Berlin June 1-5, 2008 / [s.l. : s.n., 2008]. Euro Pesticide Residues Workshop (7 : Berlin : June 1-5, 2008)
First author Ferretti, Enrica
The determination of Organochiorine Pesticides in fatty food as me^ butter, fat etc.,by gas chromatography with electron capture detector (GC-ECD), involve a very complex sample treatment. In quantitative analysis
one of the main problems is the suppression or enhancement of the analyte response caused by sample
matrix components. This can seriously affect the recovery and therefore accuracy. This parameters depend
by sample treatment and quantification procedure. To evaluate how much they influence the final recovery, it
is necessary to investigate them separately analyzing spiked blank fat where the analytes are added before
and alter the treatment of the sample. Calibration curve were performed using both solvent based and matrix
matched standards of the same concentration. We evaluated the matrix effect all over the studied calibration
range.
ID nr. 4044
Sgoifo_Rossi CA, Vandoni S, Bertocchi° L Dell'Orto V
Beef production bovine: facilities, microclimate and feeding
Inf Zootec. - Vol. 56 no 5 ( 2009). - p 38-44
Analysis of the animal adaptation of housing, microclimate and feeding in the Italian intensive farming for meat
cow production.
ID nr. 4045
Lombardi° G, Casalone C, D' Angelo A, Gelmetti° D, Torcoli° G, Barbieri° I, Corona C,
Fasoli E, Farinazzo A, Fiorini M, Gelati M, Lulini B, Tagliavini F, Ferrari S, Caramelli M,
Monaco S, Capucci° L, Zanusso G
Intraspecies transmission of BASE induces clinical dullness and amyotrophic changes
Pag. 125 di 300 31/08/2011 PLoS Pathog. - Vol. 4 no 5 ( 2008). - p 1-10. - 28 bib ref
Impact factor: 9,336
Project PRC2002003 - Experimental transmission of Italian BSE strain to cattle: clinical observation, strain
characterization, in vivo PrPres and molecular marker detection; establishment of reference samples
for Nation-wide research. - Capucci Dr. Lorenzo - Capofila - MINSAN - 2002 - 306402,10 EUR 17/11/2003 0.00.00 - 31/08/2006 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Capucci Dr.
Lorenzo - I
2 - IZSPLV - Caramelli Dr.ssa Maria - E
3 - IST. NEUROLOGICO BESTA - Tagliavini Dr. Fabrizio - E
4 - UNI VR - Monaco Dr. Salvatore - E
The disease phenotype of bovine spongiform encephalopathy (BSE) and the molecular/ biological properties
of its prion strain, including the host range and the characteristics of BSE-related disorders, have been
extensively studied since its discovery in 1986. In recent years, systematic testing of the brains of cattie
coming to slaughter resulted in the identification of at least two atypical forms of BSE. These emerging
disorders are characterized by novel] conformers of the bovine pathological prion rotein (PrPTSE) named
high-type (BSE-H) and low-type (BSE-L). We recently reported two Italian atypical cases with a PrPTS type
identical to BSE-L, pathologically characterized by PrP amyloid plaques and known as bovine amyloidotic
spongiform encephalopathy (BASE). Several lines of evidence suggest that BASE is highly virulent and easily
transmissible to a wide host range. Experimental transmission to transgenic mice overexpressing bovine PrP
(fgbov XV) suggested that BASE is caused by a prion strain distinct from the BSE isolate. In the present
study, we experimentally infected Friesian and Alpine brown cattie with Italian BSE and BASE isolates via the
intracerebral route. BASE-infected cattle developed amyotrophic changes accompanied by mental dullness.
The molecular and neuropathological profiles, including PrP deposition pattern, closely matched those
observed in the original cases. This study provides clear evidence of BASE as a distinct prion isolate and
discloses a novel disease phenotype in cattle.
ID nr. 4046
Benetti C, Angeletti R, Binato G, Biancardi° A, Biancotto G
A packaging contaminant: Isopropylthioxanthone (ITX) in dairy products
Anal Chim Acta. - Vol. 617 ( 2008). - p 132-138 - 14 bib ref
Impact factor: 3,186
A fast, simple and very selective liquid chromatography-mass spectrometry (LC-MS) method for the detection
of isopropylthioxanthone (ITX) in dairy products has been developed and validated. After addition of an ITX-d3
as internal standard and a simple extraction from the sample with acetonitrile, the extract was centrifuged and
directly injected into the LC-MS system. Chromatographic separation was achieved by means of a Gemini
C18 column (100 mm x 2.0 mm i.d. 5 µm) using a gradient of aqueous 20 mM ammonium formiate at pH 4.5
and methanol as the mobile phase, at a flow rate of 0.25 mL min-1. The method was validated according to
the guidelines laid down by the Commission Decision 2002/657/EC using the parent ion [M+H]* (m/z 255) as
quantification ion, and the fragment ion (m/z 213) obtained by in-source collision-induced dissociation (IS-CID)
as confirmation ion. Absolute and relative recoveries rates were verified at 5, 10, 15 µgkg-1 in yoghurt
samples and at 5 µgkg-1 in milk and pudding: mean absolute recoveries were 77% in yoghurt, 50% in pudding
and 67% in milk; relative recoveries (after intemal standard correction) were always >97% in each matrix. The
detection limit (CCa) and the detection capability (CCP) of method were 6.2 and 7.2 µgkg 1, respectively.
ID nr. 4047
Camarda A, Circella E, Pennelli° D, Battista P, Di_Paola G, Madio A, Tagliabue° S
Occurrence of pathogenic and faecal Escherichia coli in layer hens
Ital J Anim Sci. - Vol. 7 ( 2008). - p 385-389. - 7 bib ref
Impact factor: 0,2180
Pag. 126 di 300 31/08/2011 A total of 117 Escherichia coli from colibacillosis affected (APEC) and clinically healthy birds (AFEC) were
serotyped and tested for the presence of virulence genes: iss, tsh, cva. A total of 54.5% E. Coli were typeable
and 15 different serogroups were identified. The most common serogroups among APEC strains were 078, 02
and 0128, whereas 0139 was predominant in faecal strains from healthy birds. Iss, tsh e cva were more
frequently detected among the septicaemic E. coli strains. The association of virulence genes was observed.
Particularly, the pathotype iss-tsh-cva was present in 46.5% of APEC strains. Referring to serogroups, E. coli
078 and 02 originating from colibacillosis affected birds were always isstsh-cva positive but did not share
virulence genes when they came from healthy birds.
ID nr. 4048
Calisesi L, Cappuccio P, Pozzi P, Fiorentini° L
Evaluation of the efficacy of an Haemophilus parasuis vaccine through a field clinical investigation
Atti Convegno SIPAS. - Vol. 34 ( 2008). - p 283-291. - 11 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (34. : Salsomaggiore
Terme (PR) : 13-14 marzo 2008)
A field trial has been conducted with the purpose to appraise the effectiveness of a commercia) vaccine
aganist Glsser's disease. During the test 2486 piglets from the same firm (site 1) have been valued. They has
divided in three groups: - Vaccinated pigs born by not vaccinated mothers (SV),
- Not vaccinated pigs born by vaccinated mothers (MV),
- Not vaccinated pigs (C).
Piglets have been checked during the permanence in site 2 and for the first 24 days in site 3. Through weekly
observations of all groups, percentge of piglets with clinical symptoms referable to Glsser's disease has been
recorded. Besides we have performed laboratory and necropsy examinations on dead animals. From the data
obtained we can conclude that a significant clinical variation was relative to articular pathologies in MV group.
ID nr. 4049
Moreno_Martin° A, Barbieri° I, Chiapponi° C, Foni° E, Sozzi° E, Canelli E, Luppi° A,
Cordioli° P
Genetic characterization of H1N1 and H1N2 swine influenza viruses isolated in Italy in 1998-2007
Second Annual meeting Epizone : "Need for speed" : 4,5 and 6 - June, 2008 Brescia, Italy : programme and
abstracts / [s.l. : s. n., 2008]. - p 99. - 3 bib ref
Annual meeting Epizone (2nd : Brescia, Italy : 4,5 and 6 - June, 2008)
First author Moreno_Martin, Ana
Several changes occurred in the epidemiology of swine influenza viruses (SIV) in Europe in the last years.
Classical 1-11N1 were replaced in 1979 by "avian like" H1N1SIVs. The H1N2 subtype was first described in
Great Britain in 1994 and since then spread to continental Europe. In this paper the genetic relationships
between 29 Italian 1-11N1 and, 12 H1N2 SIVs isolated from 1998 to 2007 were investigated. Material and
Methods Partial HA and NA amplification and sequencing were performed as reported(1). Phylogenetic
analysis was conducted by MEGA4(3). Results HA sequences of Italian 1-11N1 SIVs belonged to two groups:
1) strains isolated in the '90s closely related to sw/Fin/2899/82, 2) recent isolates similar to avian-like
sw/IV/1455/99 (2). N1 sequences of ali recent strains, except three, were closely related to Fin/2899/82 and
some earlier Italian strains. The remaining 3 isolates were like IV/1455/99 viruses. HA sequences of H1N2
SIVs were clearly distinguishabie from H1N1 SIVs. Italian strains isolated in 1998-2003 appeared similar to
contemporary H1N2 SIVs from Northern Europe and formed a cluster separated from that of the recent Italian
strains. Interestingly, one reassortant strain It/5433/01 1-11N1 was identified (97% identity to H1N2 Italian
SIVs). N2 sequences of the earlier isolates were closely related to current strains isolated in France and
Belgium and were in a different cluster from recent Italian strains (human-iike sw/Fin/127/99 SIVs). The NA
gene of strain It/11271/03 H1N2 exhibited a high similarity to the Italian H3N2 SIVs. Discussion These resuits
Pag. 127 di 300 31/08/2011 pointed out homogeneity among the recent 1-11N1 SIVs as well as distinguishing these from the earlier
strains. It should be noted that while HA gene of recent H1N1 SIVs was different from that of the early
circulating strains, the NA gene seeméd to be more related to them. Also for Italian H1N2 the presence of a
homogeneous group, composed of the recent viruses, differing from earlier strains could be described. Finally
the occurrence of reassortment events was evidenced by the detection of two reassortant strains. These
findings suggest the requirement for improved surveillance activities in the future.
ID nr. 4052
Rosignoli° C
Clostridial diseases in cattle : diagnosis and control
Buiatria. - Vol. 3 no 4 ( 2008). - p 27-56. - 75 bib ref
Project PRC2001006 - Use of PCR for typing C. perfrigens strains isolated from enteric and entero-toxiemic
disease cases of animals and animal source foods. - Rosignoli Dr. Carlo - Solo - MINSAN - 2001 93163464,00 LIT - 03/03/2003 0.00.00 - 02/07/2004 0.00.00 - 1 - M6 - Mantova - Rosignoli Dr.
Carlo - I
First author Rosignoli, Carlo
Clostridiosis are diseases caused by toxigenic, Gram positive, anaerobic bacteria of genus Clostridiiim. In
cattle the most important clostridial diseases are those that cause "sudden death" or "unexpected death" such
as enterotoxaemias and tissue infections sustained by histotoxic clostridia. In recent years also severe
outbreàks of botulism have often been observed both in dairy farms and fattening units, with significant
economic losses due to the number of animals affected and to the public health restrictive actions undertaken.
Outbreaks of tetanus instead are* sporadic and isolated, while intestinal infections in calves sustained by
Clostridium difficile are currently under study and investigation. To manage these diseases the practitioner
needs to follow the appropriate diagnostic routes and to put into place effective control programmes. The aim
of the present paper is to consider these aspects by giving a brief introduction to the etiopathogenesis of the
different diseases.
ID nr. 4054
Barbieri° I, Brocchi° E, Campagna° D, Capucci° L
Ph variations affect PK stability of guanidine-treated BSE and BASE-associated PrP
Prion2008 : 08-10 October 2008, Madrid, Spain : book of abstracts / [Spain : s.n., 2008]. - p 126
Prion2008 : Madrid, Spain : 08-10 October 2008)
First author Barbieri, Ilaria
Background: The agent responsible for Bovine Spongiform Encephalopathy may exist in at least 3 different
forms referred to as C-Type, H-Type and L-Type based on electrophoretic mobility and glycoprofiling of PrPs,.
Passages in conventional mice and inoculation in cows has shown diversity in bovine prion strains in terms of
incubation period and lesion profile strengthening the hypotesis that, as for human TSE, each prion conformer
carries the specific information that determine its own distinct biological properties.
Objective: We investigated biochemical and physicochemical properties of PrPsc associated to classical BSE
(C-type) and BASE (L-type) Italian isolates in order to unravel differences in their molecular structure.
Methods: Aliquots of BSE and BASE terminally diseased brain homogenates were incubated with increasing
concentration of GdnHCI at different pH values followed by limited proteolysis with PK. Then samples were
precipitated with methanol and subjected to Western blot with a set of MAbs.
Results and Discussion: Treatment with 2.5 M GdnHCI pH 7.6led to a significant increase in PK sensitivity of
L-type PrP compared to C-type that showed resistance to PK up to 3.5 M GdnHCI. Interestingly, when
GdnHCI solutions at pH 3.5 were used an increased stability to PK of both C-type and L-type PrP was
observed as they were digested at GdnHCI concentration of 4.5 M and 4.0 M respectively. These data
indicate the possibility of discriminating C-type and L-type PrP on the base of their distinct stability to PKinduced proteolysis following exposure to guanidine. They also suggest the existence of differences in their
Pag. 128 di 300 31/08/2011 conformational properties that seem to be affected by pH variations. Assessing the extent of such biochemical
diversity may provide hints to understand how differences in the molecular structure of C-type and L-type PrP
are related to the distinct pathological phenotype of BSE and BASE.
ID nr. 4055
Benedetti D, Fiorini M, Cracco M, Ferrari S, Capucci° L, Brocchi° E, Monaco S, Zanusso G
Molecular characterization of low molecular mass C-terminal fragments in different CreutzfeldtJakob disease subtypes
Prion2008 : 08-10 October 2008, Madrid, Spain : book of abstracts / [Spain : s.n., 2008]. - p 155
Prion2008 : Madrid, Spain : 08-10 October 2008)
Background: In sporadic Creutzfeldt-Jakob disease (sCJD) the clinical variability has not been fully explained
by molecular studies relating two major types of PrP21-30 with unglycosylated peptides of 21(type 1) and 19
kDa (type 2) and the amino acid methionine or valine at position 129. In a previous work, by using twodimensional immunoblot we identified distinct N-terminal truncated forms of prion protein in different sCJD
subtypes. Objective: In the present study, we searched on low molecular mass PrPs, fragments (below
10kDa) which might correlate with the phenotypic variability observed in different sCJD molecular subtypes.
Methods: Brain homogenates of sCJD subjects were separated by mono- and two-dimensional
electrophoresis and immunoblotted by using anti-PrP antibodies directed to N- and C-terminus epitopes.
Results and Discussion: We biochemically characterized by mono- and two-dimensional analyses novel Cterminal PK-resistant fragments migrating at -5.5 kDa and with an isoelectric point around 4. These fragments
were found in almost all different sCJD subtypes with minor variabilities among subjects. These data show the
presence of multiple PrPs, conformations in sCJD and, in addition, shed new tight on the correlation between
sCJD phenotypes and disease associated PrP molecules.
ID nr. 4056
Rota_Nodari° S, Candotti° P
The effect of stressors on the onset of gastric ulcers in swine
Large Anim Rev. - Vol. 15 Supp al n 3 ( 2009). - p10-12. - 7 bib ref
Congresso Nazionale Multisala SIVAR (11. : Cremona : 8-9 maggio 2009)
First author Rota_Nodari, Sara
The author review the stressors currently present in pig farming and their effects on the onset of
gastroesophageal ulcer in swine.
ID nr. 4057
Rota_Nodari° S, Candotti° P
A scoring system for gastric ulcers
Large Anim Rev. - Vol. 15 Supp al n 3 ( 2009). - p15-17. - 2 bib ref
Congresso Nazionale Multisala SIVAR (11. : Cremona : 8-9 maggio 2009)
First author Rota_Nodari, Sara
The authors present a method to classify the gastroesophageal ulcers of swine. Thew methos is based on a
0-4 scoring system.
Pag. 129 di 300 31/08/2011 ID nr. 4058
Vergerio° EE
Formazione veterinaria an amazing challenge
30 giorni. - Vol. 2 no 6 ( 2009). - p 25-26.
First author Vergerio, Erika_Ester
Since October 2008 “Animal welfare of animals kept for farming purposes” the first e-learning course hosted
on “Formazione veterinaria”, the LMS platform made by IZSLER’s Reference Centre of Training Course in
Veterinary Public Health in collaboration with Ministry of Health, has been atttended by almost 1014
students. The course was carried out for veterinarians, otherwise at the end of January it has been opened to
everybody intersted in animal welfare. This review consideres LMS platfom experience since 6 months of
activity taking in account the most important issues met by the students during his use (registration, users
account, password loosing, watching and listening lessons and passing final exam) +-.
ID nr. 4060
Tamba° M, Fontana° MC, Leonelli° R, Santi A, Martini E, Barigazzi° G, Bardasi° L, Dottori°
M
Yersinia enterocolitica O:9 and false positive reactions to Bovine Brucellosis serological tests
Osservatorio. - Vol. 12 no 3 ( 2009). - p 4-5
Project PRC2005007 - Study of the unspecific reactions in the diagnosis of bovine brucellosis - Dottori Dr.
Michele - Solo - MINSAN - 2005 - 109600,00 EUR - 05/06/2006 0.00.00 - 04/06/2008 0.00.00 1 - N8 - Reggio Emilia - Dottori Dr. Michele - I
2 - G2 - Reparto di Batteriologia Specializzata - D'incau Dr. Mario - I
3 - N4 - Modena - Barigazzi Dr. Giuseppe - I
4 - N0 - CEREV - Tamba Dr. Marco - I
5 - T1 - OEVRL - Zanardi Dr. Giorgio - I
6 - M2 - Bergamo - Paterlini Dr. Franco - I
First author Tamba, Marco
Yersinia enterocolitica (YE) O:9 causes false positive reactions to Bovine Brucellosis serological tests. A
survey on YE prevalence was carried out in cattle in Emilia-Romagna. Between may 2006 and april 2008
1939 single faeces samples were collected and tested for YE presence. Cattle tested were divided in two
groups: (A) heads positive to Brucellosis complement fixation test (n. 151), and (B) heads negative to
Brucellosis complement fixation test (n. 1290) YE was detected in 72 animals. YE prevalence were equal to
35.1%in group A and equal to 1.5% in group B. 100% of YE isolated in group A and 42% in group B were
identified as O:9. In Emilia-Romagna YE O:9 seems strictly related to false positive reactions to brucellosis
test.
ID nr. 4063
Pennelli D, Salogni° C, Tagliabue° S, Alborali° L
Antibiotic resistance in Lactococcus garvieae strains isolated from salmonid fishes during the
period 1994-2005: preliminary results
Osservatorio. - Vol. 12 no 3 ( 2009). - p 13-15.
In Italy Lactococcosis is considered the main liable for yearly shrinkage of trout production. Antibiotics are
used to control the disease, even if it is necessary to consider that a wrong employment may cause a
development of antibiotic resistance. Our aim is to provide a first share to in vitro Lactococcus garvieae
antimicrobial resistance study; we examined 73 strains isolated from rainbow trout (Oncorhynchus mykiss)
during the period 1994-2005, reared in farms placed in the north Italy. It has been applied the Kirby-Bauer
Pag. 130 di 300 31/08/2011 agar diffusion method; each strain was tested for 27 different antimicrobial drugs. All the strains were
flumequine (100%) resistant; high rates of drug resistance were detected for colistin, tiamulin (97,3%) and
sulfadiazine (96%). Full susceptibility (100%) was observed for amoxicillin, clavulanic acid + amoxicillin and
gentamici.
ID nr. 4064
Vergerio° EE
E-learning course training on animal welfare
30 giorni. - Vol. 1 no 9 ( 2008). - p 9-13.
First author Vergerio, Erika_Ester
In 2008 IZSLER’s Reference Centre of Training Course in Veterinary Public Health in collaboration with
Ministry of Health has set up a LMS platform that hostes e-learning course for veterinarians.“Animal welfare
of animals kept for farming porpuses “ was the first e-learning started up. Veterinarians could attend it
connecting to www.formazioneveteria.it . This learning way was joined with a new called “autofomazione”
based on use of sms to course registration and to take on the final examination.
ID nr. 4065
McPhee SR, Butler KL, Kovaliski J, Mutze G, Capucci° L, Cooke E
Antibody status and survival of Australian wild rabbits challenged with rabbit haemorrhagic disease
virus
Wildl Res. - Vol. 36 no 5 ( 2009). - p 447-456. - 30 bib ref
Impact factor: 1,111
In Australia, the epidemiology of rabbit haemorrhagic disease virus (RHDV) is complicated by non-pathogenic
forms of calicivirus (bCV) co-circulating with RHDV and providing variable protection from RHDV. Currently no
bCV virus-specific antibody tests exist; however, a series of four ELISAs used to detect antibodies to RHDV
provided an indirect means to detect antibodies to bCV, enabling antibody categories of seronegative,
maternal RHDV, RHDV or bCV to be determined. Rabbits (188) from four locations were challenged with
RHDV and logistic regression models determined that, for rabbits <15 months old, survival was dependent on
antibody titres alone and the relationship did not vary with age, capture site, gender, liveweight or reproductive
status. All rabbits survived challenge after reaching 15 months of age, irrespective of their antibody titres.
Where bCV antibodies were prevalent in young rabbits, the bCV category did not adequately summarise all
information about rabbit survival that can be obtained from antibody titres. Within antibody categories, 95% of
rabbits with RHDV, 33% with bCV, 40% with maternal RHDV and 22% with seronegative antibodies survived.
The high survival rate of adults implies that natural outbreaks or controlled releases of RHDV will have little
impact on adult breeding rabbits. Therefore, where RHDV and bCV are endemic, conventional rabbit-control
programs targeting the immune breeding populations should provide the most predictable outcome for longterm maintenance of low rabbit populations.
ID nr. 4066
Amadori° M, Stefanon B, Sgorlon S, Farinacci M
Immune system response to stress factors
Ital J Anim Sci. - Vol. 8 suppl. 1 ( 2009). - p 287-299. 49 bib ref
Impact factor: 0,132
First author Amadori, Massimo
Pag. 131 di 300 31/08/2011 This review highlights fundamental mechanisms of the stress response and important findings as to how the
immune system is affected and affects, in turn, such a response. The crucial link between stress response
and energy metabolism is dealt with as well. The effector mechanisms in the stress response are remarkably
similar for both infectious and non–infectious stimuli, albeit differently modulated. “Psychosensitive
stimuli/behavioural response” and “Antigenic stimuli/immune response” are indeed two subsystems of a
unitary, integrated complex aimed at providing optimal conditions for the host’s survival and adaptation. The
interaction between the immune system and the stress/inflammation complex has led to the development of a
diversified network of cytokines and chemokines in vertebrate animals. The cytokine response can be
mounted in different forms and extent by the host after exposure to both infectious and non-infectious stimuli.
In this conceptual framework, microbial infections are just one category of stressing agents, which modulate
the cytokine response for a better performance of the innate and adaptive immune responses. The response
to infectious and non–infectious stress leads to a metabolic shift that enhances energy, amino acids and
micronutrients consumption. The influence of each nutrient on different aspects of immune function is not
easy to define, but it is becoming clear that many nutrients have defined roles in the immune response and,
accordingly, their requirements are changed to support optimal immune function. Therefore, impairment of
immune functions may arise from intakes of nutrients below or above these modified ranges of requirements.
ID nr. 4067
Scarcella C, Carasi S, Cadoria F, Macchi L, Pavan A, Salamana M, Alborali° GL, Losio°
MN, Boni° P, Lavazza° A, Seyler T
An outbreak of viral gastroenteritis linked to municipal water supply, Lombardy, Italy, June 2009
EuroSurveillance http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=19274 - ultimo accesso
27/07/2009. - Vol. 14 no 29 ( 2009). - 3 p. - 1 bib ref
We report an outbreak of viral gastroenteritis linked to municipal drinking water in a town in northern Italy in
June 2009. Over one month we identified 299 probable cases of whom 30 were confirmed for at least one of
the following viruses: norovirus, rotavirus, enterovirus or astrovirus. Water samples and filters from the water
system also tested positive for norovirus and enterovirus. Control measures included treating the water
system with chlorine dioxide and filters with peracetic acid, while providing temporary alternative sources of
drinking water to the population.
ID nr. 4068
Tamba° M, Rugna° G, Galletti° G, Luppi° A, Piccolomini LL
Incidence of natural infection due to vaccinal strain of bluetongue virus serotype-2
Argom. - Vol. 11 no 1 ( 2008). - p 59-60. - 2 bib ref
Project PRC2004003 - Epidemiologic and entomologic investigations for the control of Blue tongue disease
(BT) in an area which is free from the disease but endemic for a BT virus of vaccinal origin - Tamba
Dr. Marco - Solo - MINSAN - 2004 - 63020,00 EUR - 01/07/2005 0.00.00 - 30/06/2007 0.00.00 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Reggio Emilia - Dottori Dr. Michele - I
3 - N4 - Modena - Pongolini Dr. Stefano - I
4 - N3 - Forlì - Massi Dr.ssa Paola - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Tamba, Marco
A incidence survey was carried in 2006 out in a montain area of Emilia-Romagna involved since 2004 in
natural circulation of vaccinal strain of bluetongue virus serotype 2 (BTVV-2). BTVV-2 seroconversion was
detected in 4 out of 1287 cattle tested. BTVV-2 incidence in 2006 was 0.3% lower than values estimated both
in 2004 (42.2%) and in 2005 (1.8%). After two years since introduction in the area BTVV-2 is still circulating.
Pag. 132 di 300 31/08/2011 ID nr. 4074
Zanardi° G
Human Mycobacterium bovis infection in the United Kingdom: incidence, risk, control measures and
review of the zoonotic aspects of bovine tuberculosis
Osservatorio. - Vol. 12 no 2 ( 2009). - p 4-14
First author Zanardi, Giorgio
Human infection with M. bovis in the UK has been largely controlled through pasteurization of cows’ milk and
systematic culling of cattle reacting to compulsory tuberculin tests. Nowadays the majority of the 7000 cases
of human TB annually reported in the UK are due to M. tuberculosis acquired directly from an infectious
person. In the period 1990–2003, between 17 and 50 new cases of human M. bovis infection were confirmed
every year in the UK. This represented between 0.5% and 1.5% of all the culture-confirmed TB cases, a
proportion similar to that of other industrialized countries. A significant risk of M. bovis infection remains in
certain segments of the UK population in the form of (i) continuing on-farm consumption of unpasteurized
cows’ milk, (ii) retail sales by approved establishments of unpasteurized milk and dairy products and (iii)
occupational exposure to infectious aerosols from tuberculous animals and their carcasses..
ID nr. 4075
Nigrelli° AD, Vantini F, Camoni C, Casappa P
Effect evaluation of the aminosidine and gentamicin water medication in postweaning ETEC
diarrhoea
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 449-454. - 3 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini ( SIPAS ) (35 : Modena : 1213 Marzo 2009)
First author Nigrelli, Arrigo Daniele
The Authors verified the best effect on the E.coli post-weaning diarrhoea and on
the growth of aminosidine in piglets, in compared with gentamicin.
ID nr. 4076
Nigrelli AD°, Vantini F, Camoni C, Casappa P,
Effect evaluation of amminosidine and apramycin in feed treatment in post weaning etec diarrhoea
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 444-448. - 4 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini ( SIPAS ) (35 : Modena : 1213 Marzo 2009)
First author Nigrelli, Arrigo Daniele
The efficacy of an in feed treatment, Amminosidine based, in controlling
post-weaning diarrhoea was evaluated. The authors verified the better results of treated piglets in comparison
to the ones in feed treated with Apramycin.
ID nr. 4077
Merialdi° G, Fontana° MC, Tallarico N,Turci S, Leonelli° R., Galletti° G, Vincenzi E, Rugna
G, Bonilauri° P
Effect of pre-slaughter diet integration with a protected source of formiate and citric acid (formylr)
Pag. 133 di 300 31/08/2011 upon the prevalence of Salmonella spp carrier slaugther pigs
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 437-443. - 15 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini ( SIPAS ) (35 : Modena : 1213 Marzo 2009)
First author Merialdi, Giuseppe
This field trial was carried out to determine the effects of feed acidification with a protected source of formiate
and citric acid (FormylR) upon Salmonella carriage in marketage pigs. A group of pigs from an Italian pig herd
with high level of infection with Salmonella was included. Pigs were randomly divided into two groups: the
experimental one received for 30-50 days prior to slaughter a commercial diet supplemented with formic acid
source (Formyl), and the control pigs received the same diet but unacidifi ed. At slaughter 100 pigs (control
group) and 104 pigs (experimental group) were submitted to collection of caecal content and mesenteric
lymph nodes for Salmonella isolation using standard procedures. The results revealed a signifi cant decrease
of the rate of carriers in the experimental group.
ID nr. 4079
Candotti° P, Rota_Nodari° S
Straw administration bydispenser in long and tail docked pigs : evaluation of tail and ear biting
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 350-359. - 7 bib ref
Meeting annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 1213 Marzo 2009)
First author Candotti, Paolo
The influence of straw on tail and ear biting in pigs was evaluated in a site 2 commercial farm. 336 pigs tail
docked at 2 days of age and 314 pigs not taildocked were divided in 4 groups: group A (straw administered,
long tail); group B (no straw, long tail); group C (straw administered, tail docked); group D (no straw, long tail).
Straw was given between D0 (day of weaning: 28 days of life) and D47 but not between D48 and D71. Ear
and tail lesions were recorded at day 47 and 71. Straw significantly reduced tail lesions in group A compared
to group B (p<0.05) while it was administered. After the removal of the straw, the prevalence of tail lesions at
T71 did not differ between group A and B. Groups C and D did not differ significantly at T47, while at T71 the
prevalence of pigs with tail lesions was higher in group D compared to group C (p<0.05). Lesions at the apex
of the ear were significantly higher in group D compared to group B and C both at D47 and D71 (p<0.05).
ID nr. 4080
Candotti° P, Rota_Nodari° S
Surgical and functional surgery in a lactating sow affected by vulva biting
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 345-348. - 5 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Candotti, Paolo
In a farrow to finish herd with 300 breeding sows, a granparent Large White sow had her vulva bitten by her
piglets in the farrowing crate. The cannibalism induced anatomical changes consisting in a loss of most of the
vulva's labias and a subsequent closure of the interglacial sulks resulting in a reduction of the opening to
about only 3 mm. The anatomical changes had functional consequences resulting in: an abnormal urinating
behaviour, consisting in pollakiuria In order to re-establish a welfare condition and to allow a natural farrowing,
the animal underwent successfully a plastic surgery to enlarge the interlabial solcus.
Pag. 134 di 300 31/08/2011 ID nr. 4081
Archetti° I, Rota_Nodari° S, Guerra° O, Candotti° P
Reference intervals of haematological parameters in weaned piglets and sows
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 314-319. - 22 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Archetti, Ivonne Laura
ID nr. 4082
Candotti° P, Rota_Nodari° S, Razzuoli° E, Dotti° S, Amadori° M
Assessment of the inflammatory response to early weaning in piglets: impact of an oral interferonalpha treatment
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 293-299. - 8 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Candotti, Paolo
ID nr. 4083
Luppi° A, Bonilauri° P, Mazzoni C, Spaggiari° B, Maioli° G, Leonelli° R, Di_Lecce R, Borri
E, Tonon F, Gradellini S, Ferrari E, Dottori° M
Diagnostic approach before and after vaccination for PCV2 in a subclinical PCVAD/PMWS
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 249-257. - 12 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Luppi, Andrea
A field study was conducted to investigate the use of a diagnostic approach, based on lymph-nodal cytology
and sera and lymph-nodal Real Time PCR on pigs, after and before the vaccination against PCV2. This
approach can help the understanding of PCVAD (Porcine Circovirus Associated Disease) and sub-clinical
PCV2 infection herds severity. The diagnostic approach was performed in three different periods, on the set
up of health problems, due to PCVAD/PMWS, five months later and after the vaccination. This study showed
high sera and lymph-nodal Real Time PCR viral load in 12 weeks old pigs, not always with a confirmatory
diagnosis of PCVAD/PMWS, before the vaccination in the herds. 3 weeks old pigs vaccinated didn’t show
viremia at 5, 12 and 19 of age. The vaccination and the diagnostic approach results need of further
investigations..
ID nr. 4084
Faccini° S, Rosignoli° C, Franzini° G, Nigrelli° AD
Preliminary evaluation of importance of DNA extraction method for PCV2 quantitative Real-Time PCR
data
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 243-248. - 12 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Faccini, Silvia
Quantitative Real-Time PCR has become an important tool for PCV2 research and clinical diagnosis. DNA
extraction from clinical samples is unquestionably a very critical pre-analytical step. Yield, repeatability, purity,
and removal of PCR inhibitors undoubtedly affect quantitative Real-Time PCR results and performances. This
Pag. 135 di 300 31/08/2011 study is a first evaluation of how extraction method influences PCV2 quantification in two of the most
important clinical samples: serum and lymph node. The latter results to need particular attention due to
frequent presence of PCR inhibitors in DNA extracts.
ID nr. 4086
Bosi P, Merialdi° G, Bardasi° L, Scandurra S, Vecchi M, Ferro P, Messori S, Nisi I, Casini
L, Trevisi P
Effect of three different antibiotics on commensal intestinal microflora and on some productive
traits: preliminar report
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 219-228. - 15 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
An in vivo experiment was performed to study the eff ect of three diff erent
antibiotics on intestinal microfl ora composition and on some productive parameters and in weaned piglets.
Seventy-two newly weaned conventional piglets were included and randomly assigned to 4 diff erent diets:
conventional weaning diet with no antibiotics (C), diet with tilmicosin supplementation (T), diet with amoxicillin
supplementation (A) and diet with doxiciclin supplementation (D). Th e experiment had an overall duration of 4
weeks. Antibiotics were added to diets for 3 weeks. During the experiment and at its end, data about average
daily gain (ADG) , feed intake (FI), and feed to gain ratio (FGR) were recorded. Fecal samples were collected
weekly for Enterobacteriaceae and lactobacilli. Th e groups receiving an antibiotic supplemented diet had
signifi cantly 220 higher ADG and FI values, without any impact on FGR. Lactobacilli concentration did not
result averagely impaired by antibiotics, but D and T increased it, as compared with A. Conversely
Enterobacteriaceae were strongly reduced by the tilmicosin added diet.
ID nr. 4087
Franco A, Merialdi° G, Iurescia M, Feltrin F, Lorenzetti R, Zini M, Amoruso R, Buccella C,
Bassoli O, Cito G, Cuoghi G, De_Bassa A, Floriani E, Perrone V, Razzini P, Liuzzo G,
Battisti A
Methicillin-resistant staphylococcus aureus (MRSA): survey at slaughter among holdings from Italy
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 208-211. - 7 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
Project PRC2007106 - Prevalence study of methicillin-resistant S. aureus (MRSA) in fattening pigs - Battisti
dr. Antonio - UO - MINSAN - 2007 - 0,00 EUR - 29/09/2008 0.00.00 - 28/09/2010 0.00.00 - 2 N1 - Sezione diagnostica provinciale di Bologna - Merialdi Dr. Giuseppe - I
A survey to estimate the prevalence of Methicillin-resistant Staphylococcus
aureus (MRSA) among holdings of fattening pigs was conducted in Italy in
2008. Herds were selected by a random sampling procedure and sampled at differentslaughterhouses of
northern and central Italy. Nasal swabs from batches ofanimals from each holding enrolled were randomly
sampled (double-stage samplingtechnique). Suspect isolates from pooled primary cultures were cloned and
subsequentlyscreened by PCR for species identifi cation and for the mecA gene. Positiveisolates were
submitted to spa-typing and SCCmec typing, according to internationalconsensus protocols. Among the herds
surveyed (n=118), a prevalence of 38%was estimated (CI 29-47%, 95% CL). Th e Italian pig isolates showed
heterogeneity.
ID nr. 4088
Pag. 136 di 300 31/08/2011 Giammarioli M, Canelli E°, Ciulli S, Pellegrini C, Rossi E, De_Mia GM
Genetic heterology of the budvirus in Italy
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
156-157 -10 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
The genetic heterogeneity of 111 Italian BVDV isolates was investigated by phylogenetic analysis of partial 5’UTR and for selected viruses, of the genomic region encoding autoprotease Npro. Additional sequences of
other Italian BVDV isolates were acquired from the GenBank database. At the subgroup level, pair wise
similarity and cluster analysis provided a clear-cut assignation to 10 distinct genotypes of 106 isolates typed
as BVDV-1 namely respectively BVDV-1a (n=12), BVDV-1b (n=47), BVDV-1d (n=4), BVDV-1e (n=26), BVDV1f (n=5), BVDV-1g (n=4), BVDV-1h (n=7), BVDV-1j (n=1), BVDV-1k (n=2) and BVDV- 1l (n=1). Five isolates
were typed as BVDV-2. The results provided evidence of a high BVDV genetic heterogeneity in Italy as a
result of the absence of any BVDV systematic control measures.
ID nr. 4089
Rota_Nodari°, Archetti° I, Guerra° O, Candotti° P
Reference values of haematological parameters in sows
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 199-206. - 23 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Rota_Nodari, Sara
41 healthy breeders were selected in commercial farms of Northern Italy. Blood samples were collected from
animals and tested for several parameters (Total proteins, Albumine, Globulin, Glucose, Cholesterol,
Triglycerides, ß-hydroxybutyrate, Total bilirubin, Alanine aminotransferase, Aspartate aminotransferase,
Alkaline phosphatase, gammaglutamyltransferase, Amylase, Urea, Creatinine, Creatine kinase, Lactate
dehydrogenase, Calcium, Phosphorus, Magnesium, Sodium, Potassium, Chlorine, Copper, Iron, Zinc).
Collected data were used to calculate percentile reference intervals for the selected haematochemical
parameters. The differences found in comparing our results with published data suggested that each
laboratory should establish reference values and intervals for diagnostic and research purposes.
ID nr. 4090
Galletti° E, Merialdi° G, Antonelli A, Brini E, Fusaro L, Sarli G, Fontana° MC, Martelli P
Isoimmune thrombocytopenia in neonatal piglets: a case report
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 192-198. - 10 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Galletti, Giorgio
ID nr. 4091
Spaggiari° B, Merialdi° G, Bonilauri° P, Luppi° A, Leonelli° R, Bonci M, Sandri GP, Dottori°
M, Gozio S, Martelli P
S.P.E.S. grid: slaugtherhouse lungs evaluation of pig batches from two actinobacillus
pleuropneumoniae low seroprevalence herd
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 180-185. - 10 bib ref
Pag. 137 di 300 31/08/2011 Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Spaggiari, Brunella
S.P.E.S. (Slaughterhouse Pleurisy Evaluation System), a chronic pleural lesions evaluationsystem in swine
lungs during slaughter line, was applied to fi ve swine batches from two Actinobacillus pleuropneumoniae
(App)-low seroprevalence herds. Th e herds had sporadic or not seroconversions, without App infection diff
usion and bacterial isolation during the period considered. The evaluation system, consisting in a score
ranging from 0 to 4 on the basis of pleural lesions presence, extension and location on the two lungs of an
animal, was applied to the considered herds and showed that three batches had animals with pleural lesions
scored < 2 not referable to App, and in two batches only one animal, for each batch, was scored 2 and 3
respectively, in a general context of absence of pleural lesion in the remaining batch subjects. APPI index
(Actinobacillus pleuropneumoniae Index), which express both App lesions frequency and score mean,
resulted to be out of range compared to data obtained in a previous study on a group of Italian herds aff ected
by respiratory disease. In conclusion, also in the present study S.P.E.S. has proved to be a highly reliable
evaluation system and has confi rmed that chronic pleural dorsocaudal lesions detected at slaughter are
mostly a consequence of App induced pleuropneumonia.
ID nr. 4092
Salvini S, Guadagnini G, Alborali° L
Mucopurulent conjunctivitis 8 outbreaks in fattening pigs
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 174-178. - 7 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
ID nr. 4093
Vicari° N, Mandola ML, Centorbi R, Rizzo F, Andreoli° G, Bellotti° M, Magnino° S
Detection di Chlamydiaceae in wild birds sampled in Pedmont between 2008-2009
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
268-269. - 9 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Vicari, Nadia
The aim of this study was to detect chlamydial microorganisms in samples from
wild birds (cloacal swabs and organs). One hundred and twenty samples were analysed by two different realtime and two distinct conventional PCR-RFLP. Nineteen samples tested positive for Chlamydiaceae but
negative by species-specific real-time PCR for Chiamydophila psittaci and Chiamydophila abortus. The
percentage of positive sampies detected in this survey is similar to the one reported in a recent research
carried out in France . Further investigations are under way in order to characterize the inovolved chlamydiae.
ID nr. 4094
Vicari° N, Laroucau K, Vorimore F, Barbieri° I, Sachse K, Hotzel H, Fabbi ° M, Labalestra °
l, Magnino ° S
Molecular analysis fo Chlamydial isolates from the intestine and cloacal swabs of feral pigeons
sampled in Milan and Ferrara
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
Pag. 138 di 300 31/08/2011 266-267. - 8 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Vicari, Nadia
In order to identify and characterize six chlamydial isolates from cloacal swabs and intestines of feral pigeons,
several molecular analyses were performed. DNA from the isolates was analyzed using Chlamydiaceae specific real -time PCR, Array Tube DNA microarray and species-specific molecular detection tools, i.e. realtime PCR, MLVA and complete sequences of ompA and 16S rDNA genes. The results obtained and the
phylogenetic tree show that our six isolates do not cluster with any known chlamydial species.
ID nr. 4095
Magnino° S, Colin P, Dei_Cas E, Madsen M, McLauchlin J, Nöckler K, Maradona MP,
Tsigarida E, Vanopdenbosch E, Van_Peteghem C
Biological risks associated with consumption of reptile products
Int J Food Microbiol. - Vol. 134 no 3 ( 2009). - p 163-175. - 146 bib ref
Impact factor: 2,753
First author Magnino, Simone
The consumption of a wide variety of species of reptiles caught from the wild has been an important source of
protein for humans world-wide for millennia. Terrapins, snakes, lizards, crocodiles and iguanas are now
farmed and the consumption and trade of their meat and other edible products have recently increased in
some areas of the world. Biological risks associated with the consumption of products from both farmed and
wild reptile meat and eggs include infections caused by bacteria (Salmonella spp., Vibrio spp.), parasites
(Spirometra, Trichinella, Gnathostoma, pentastomids), as well as intoxications by biotoxins. For crocodiles,
Salmonella spp. constitute a significant public health risk due to the high intestinal carrier rate which is
reflected in an equally high contamination rate in their fresh and frozen meat. There is a lack of information
about the presence of Salmonella spp. in meat from other edible reptilians, though captive reptiles used as
pets (lizards or turtles) are frequently carriers of these bacteria in Europe. Parasitic protozoa in reptiles
represent a negligible risk for public health compared to parasitic metazoans, of which trichinellosis,
pentastomiasis, gnathostomiasis and sparganosis can be acquired through consumption of contaminated
crocodile, monitor lizard, turtle and snake meat, respectively. Other reptiles, although found to harbour the
above parasites, have not been implicated with their transmission to humans. Freezing treatment inactivates
Spirometra and Trichinella in crocodile meat, while the effectiveness of freezing of other reptilian meat is
unknown. Biotoxins that accumulate in the flesh of sea turtles may cause chelonitoxism, a type of food
poisoning with a high mortality rate in humans. Infections by fungi, including yeasts, and viruses widely occur
in reptiles but have not been linked to a human health risk through the contamination of their meat. Currently
there are no indications that natural transmissible spongiform encephalopathies (TSEs) occur in reptilians.
The feeding of farmed reptiles with non-processed and recycled animal products is likely to increase the
occurrence of biological hazards in reptile meat. Application of GHP, GMP and HACCP procedures,
respectively at farm and slaughterhouse level, is crucial for controlling the hazards.
ID nr. 4096
Spaggiari ° B, Gherpelli ° Y, Carnevali L, Luppi ° A, Bonilauri ° P, Dottori ° M
Antibiotic-resistance in Gram-negative bacteria isolated from ear swabs of individually owned dogs
and kennel dogs
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
250-251. - 8 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Pag. 139 di 300 31/08/2011 Settembre - 2 Ottobre 2009)
First author Spaggiari, Brunella
During routine activity of IZSLER-Reggio Emilia General Diagnostics Laboratory 386 earswabs werecollected
from individually owned dogs and kennel dogs. Samples were cultured and bacterial and yeast populations
were isolated. Gram-negative bacteria were identified by bacteriological methods and tested for antimicrobial
susceptibility. This study aims at determining and comparing antimicrobial susceptibility patterns of Gramnegative bacteria between individually owned and kennel dogs.
ID nr. 4097
Rugna° G, Merialdi° G, Ramini° M, Accurso° D, Gelmini° L, Mazzini C, Bacchi M, Pozio E
Inactivation of trichinella spiralis larvae in fermented italian salami
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
234-235. - 9 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Rugna, Gianluca
ID nr. 4100
Merenda° M, Cevidalli AE, Barigazzi° G
In vitro susceptibility of 38 strains of A. pleuropneumoniae biotype 1 isolated during 2009 to 16
antimicrobials
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
190-191 - 4 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Merenda, Marianna
Thirty-eight italian Actinobacillus pleuropneumoniae isolates recovered from pigs with pleuropneumoniae
were tested for susceptibility to 16 antimicrobials.
All the isolates were susceptible to Ceftiofur and Enrofloxacin and most of them to Danofloxacin, Gentamycin,
Tiamulin, Tulatromycin and Tilmicosin. Six isolates were resistant to Florfenicol and a high rate of resistance
was observed to Tetracycline. The distribution of isolates over the MIC range observed for Penicillin and
Ampicillin, suggested the development of resistance.
ID nr. 4101
Luppi° A, Fontana° MC, Galletti° E, Spaggiari° B, Maioli° G, Bonilauri° P, Dottori° M,
Trocchi V, Merialdi° G
European brown hares (lepus europaeus) toxoplasmosis diangosis
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
176-177. - 3 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Luppi, Andrea
Pag. 140 di 300 31/08/2011 Six cases of fatal acute toxoplasmosis were observed in hares in different areas of Emilia Romagna region
during a period of 4 months. The most characteristic lesion in all hares was a severe splenomegaly.
Toxoplasmosis was diagnosed through cytological examination of spleen imprint sampled slides and
confirmed by PCR in all hares. Hares should be considered exceptionally susceptible to primary Toxoplasma
gondii infection. Epidemiology and prevalence of T. gondii in hares in Northern Italy needs further
investigations. The monitoring of the disease in hares could be used to evaluate the level of environmental
oocysts contamination in specific geographic areas..
ID nr. 4102
Luppi° A, Maioli° G , Spaggiari° B, Gelmetti° D, Gibelli° L.R, Bonilauri° P, Dottori° M
Cyathostoma bronchialis in Stenibach geese
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
174-175 - 5 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Luppi, Andrea
The gapeworms are small nematodes worms that live in the respiratory tract of some birds. Gapeworms may
cause disease in several birds, but are particularly important in pheasants, range-reared turkeys, geese and
waterfowl. The gapeworm Cyathostoma bronchialis is a round red worm that attach to the trachea of birds and
causes the disease referred to as "gapes". The term describes the open-mouth breathing characteristic of
gapeworm-infected birds. C. bronchialis appear bright red sometimes in a Y configuration (the smaller male is
attached to the larger female). An excess of mucus occurs as a result of irritation of the tracheal lining. Heavily
infected birds usually emit a grunting sound because of the difficulty in breathing and many die from
suffocation. The worms can easily block the trachea and bronchi, so they are particularly harmful to young
birds. Aim of this paper is to present a case of disease by tracheal worms in eight two months old Steinbach
geese.
ID nr. 4104
Nigrelli° AD
Enzootic enteritis in the suckling piglets with reagard to Clostrdial infection
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 134-141. - 73 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Nigrelli, Arrigo Daniele
Etiology,pathogenesis ,lesions,syntoms,prophylaxis and management factors of the enzootic enteritis in the
suckling piglets are considered.
ID nr. 4106
Chiapponi° C, Zanni° I°, Garbarino° C°, Barigazzi° G, Foni° E
Evaluation of the CACO-2 cell line for isolation of swine influenza virus compared to standard
methods
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
112-113. - 10 bib ref
Pag. 141 di 300 31/08/2011 Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
Project ESNIP2 - "European surveillance network for influenza in pigs 2 (ESNIP 2). - Van Reet dr.ssa
Kristien - UO - CE - 2005 - 300000,00 EUR - 01/01/2006 0.00.00 - 31/03/2009 0.00.00 - 4 - N5
- Parma - Foni Dr.ssa Emanuela - I
PRC2005016 - Epidemiologic surveillance of influenza virus circulation in domestic and wild animals:
detection, antigenic and genomic characterisation of viruses circulating in susceptible species of
domestic animals - Barigazzi Dr. Giuseppe - Solo - MINSAN - 2005 - 05/06/2006 0.00.00 04/06/2008 0.00.00 - 77500,00 EUR 1 - N5 - Parma - Foni Dr.ssa Emanuela - I
2 - N8 - Reggio Emilia - Dottori Dr. Michele - I
3 - N3 - Forlì - Massi Dr.ssa Paola - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
5 - M6 - Mantova - Nigrelli Dr. Arrigo - I
First author Chiapponi, Chiara
During a swine influenza virus (SIV) monitoring programme, 111 samples were submitted to virus isolation
using embryonated chicken eggs (ECE), MDCK cells and CACO-2 cells and 67 SIVs were isolated. The use
of CACO-2 cells was able to isolate 100% of H1N1 and H1N2 subtypes, while the isolation rate for H3N2 was
52%. ECE showed to be able to isolate H1N1 in 41%, H1N2 in 9% and H3N2 in 100% of the cases. MDCK
cells permitted SIV isolation in 52% of H1N1, 6% of H1N2 and 42% of H3N2.
ID nr. 4107
Bardasi° L, Giommi E, Fontana° MC, Galletti° G°, Merialdi° G,
Comparison between international standard methods ISO 6579:2002 and ISO 6579:2002/amd.1:2007
for isolation of salmonella in Turkey carcasses
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
87-88. - 7 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Bardasi, Lia
Salmonella spp. is an important cause of foodborne disease in humans. The aim of the present study was to
compare the ability of the international standard method ISO 6579:2002 used for isolation of Salmonella spp.
in poultry carcasses (neck skin samples) to ISO 6579:2002/Amd.1:2007 , used for isolation of Salmonella spp.
in faeces and environmental samples. 235 samples of turkey neck skin were examined for Salmonella spp..
The results obtained with two method showed no differences: k =0,93 (IC 95%: 0,88 – 0,98).
ID nr. 4110
Sozzi° E, Boniotti° B, Thuer B, Hofmann M, Moreno° A, Lelli° D, Fontana° R, Martinelli° N,
Lombardi° G, Cordioli° P, Lavazza° A
Investigation on the presence of Toggenburg virus (TOV) in Italy
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
56-57 - 5 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Sozzi, Enrica
Pag. 142 di 300 31/08/2011 The novel bluetongue-like orbivirus, named Toggenburg virus (TOV), was detected in a healthy goat in Italy
by serological and virological methods. Experimental infections of goats, sheep and calf, using TOV-positive
blood samples, were performed. Animals did not show any clinical or pathological signs but antibodies and
viral RNA were detected in blood samples of experimentally infected goats and sheep. Further investigations
on the prevalence of this virus in Italy are needed to improve the knowledge on its epidemiology.
ID nr. 4112
Giammarioli M, Canelli° E, Ciullis, BazzucchI M, De_Mia gm
The extended genetic heterogeneity of BVDV-1: typing of the BVDV isolates from Italy
Annual meeting of the national swine fever laboratories : June 15-16th, 2009 Valdeolmos, Spain / [s.n. : s.l.,
2009]. Annual meeting of the national swine fever laboratories : Valdeolmos, Spain : June 15-16th, 2009)
Bovine viral diarrhoea virus (BVDV), the causative agent of BVD and mucosal disease, is an economically
important pathogen of cattle. Up to date, 13 genotypes of BVDV-1 are known (la-1 m) [4] and 2 putative
additional genotypes namely In and Io, have been recently reported in Japan [5]. Studies on the prevalence of
BVDV in Italy have been conducted providing evidence of circulation of 9 BVDV-1 genotypes [1, 2, 3]. Aim of
this work has been to type 111 BVD viruses collected during the period 1995-2009 from 12 Italian regions.
Additional sequences of other Italian BVDV isolates were acquired from the GenBank database. The viruses
analyzed in this study were from cattle (n=106), sheep (n=4) and buffalo (n=4), mostly originated from farms
located in northern Italy which is characterised by the highest cattle population density in the country. The
genetic heterogeneity of the Italian BVDV viruses was investigated by phylogenetic analysis of partial 5'-UTR
and for selected viruses, of the genomic region encoding autoprotease Npro. Five isolates were typed as
BVDV-2. The remaining isolates were typed as BVDV-1 and belonged to 10 distinct genotypes namely
respectively BVDV-la (n=12), BVDV-1b (n=47), BVDV-1d (n=4), BVDV-le (n=26), BVDV- 1 f (n=5), BVDV-1g
(n=4), BVDV-1h (n=7), BVDV-lj (n=1), BVDV-1k (n=2) and BVDV-11 (n=1). To confirm the grouping found in
the 5'-UTR, we analysed in the NPr° region 19 viruses selected on the basis of their bootstrap value. The
resulting phylogenetic tree showed that these viruses were clustered in the same phylogenetic branches as
for the tree based on the 5'-UTR, with similar bootstrap values. The phylogenetic analysis provided a clear-cut
assignation to 10 distinct genotypes of 106 isolates typed as BVDV-1. Most cattle farms were infected by the
predominant BVDV-1b and BVDV-le isolates, the others genotypes occurred only sporadically. The results
also provided evidence for circulation of BVDV-11 additional genotype, which has been never shown before in
Italy. In summary, our study revealed a high BVDV genetic heterogeneity in Italy as a result of the absence of
any BVDV systematic control measures and also demonstrated that when bigger collection of BVDV isolates
was analysed, higher genetic diversity of viruses may be revealed with possibility to identify new subtypes.
ID nr. 4113
Chiapponi° C, Re M, Pierantoni M, Mazza G, Barigazzi° G, Foni° E
Study of the contamination by Listeria monocytogenes and Listeria spp in ham plant production by
microbiological and biomolecular methods.
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
16 -17. - 1 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Chiapponi, Chiara
A plant for the production of Parma Ham that had shown cases of Listeria monocytogenes contamination was
monitored for the presence of Listeria monocytogenes and Listeria spp by microbiological and bio molecular
methods.
Along the production process, 193 samples were collected and examined according to FSIS USDA (MLG
Pag. 143 di 300 31/08/2011 8.06) method and by Real-Time PCR method. This method was able to detect more positive samples than the
microbiological one. The study permitted to identify some critical points of the
production process that were corrected or removed.
ID nr. 4115
Canelli° E, Tittarelli° C, Barbieri° I, Cerutti G, Pennelli° D, Lavazza° A
Identification and genetic caracterization of avian astrovirus
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
29 -30. - 6 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Canelli, Elena
Astroviruses are non-enveloped SRVs. In this study we analyzed 318 samples of intestinal content conferred
to the laboratory since 2008. The samples were analyzed firstly by negative staining electron microscopy
(nsEM) and 68 samples were found positive for entero-like or astro-like viruses. All these samples were
analyzed with RT-PCR, searching for astrovirus RNA, and, if positive, sequenced and genetically analyzed.
Primers used for PCR and sequencing target ORF1b. The obtained data demonstrate that this gene presents
a certain genetic variability, even among astroviruses of the same species.
ID nr. 4118
Trevisi E, Amadori° M, Bakudila AM, Bertoni G
Metabolic changes in dairy cows induced by oral, low-dose interferon-alpha treatment
J Anim Sci. - Vol. 87 n 9 ( 2009). - p 3020-3029. - bib ref 31
Impact factor: 2,123
Correspondig author Amadori, Massimo
Many apparently healthy cows show marked inflammatory conditions around calving, associated with
endocrine and metabolic changes. To prevent the above conditions, a low-dose, oral interferon- (IFN- )
treatment was carried out on periparturient, multiparous dairy cows. In the first trial, 10 cows received 10 IU of
IFN- /kg of BW daily during the last 2 wk of pregnancy. In a second trial, 4 cows received 0.5 IU of IFN- /kg of
BW daily until d 5 of lactation. In both trials, a homogenous group of untreated dairy cows was used as
control. All cows were monitored, during the month before and after calving, for health status, BCS, milk yield,
and inflammatory, metabolic, immune, and hematological variables. Compared with control cows, IFN- treated animals showed in both trials a larger decrease of BCS along with decreased milk yield (P < 0.05),
increased haptoglobin (P < 0.05) and ceruloplasmin, and a slower increase of negative acute phase proteins
(albumin, cholesterol, paraoxonase, vitamin A) after calving. Interferon- -treated animals also showed a larger
decrease of plasma glucose and greater values of NEFA, ß-hydroxybutyrate, and reactive oxygen
metabolites. There also was evidence of IL-6 and tumor necrosis factor- responses in both groups before
calving with a quick decrease thereafter. The IL-6 response appeared in some animals regardless of the IFNtreatment. Results indicate that low-dose IFN- can sustain an inflammatory response in dairy cows and cause
notable metabolic changes. This outcome might be explained by the repeated and extended interaction of
IFN- at low doses with the oral lymphoid tissues during rumination, as suggested by the observed stability of
the cytokine in the rumen milieu; the final inflammatory effect could thus be as large as that of high doses. In
addition, the antiflogistic signal of IFN- might be counteracted and inverted by lymphocytes detected in the
rumen liquor.
ID nr. 4119
Pag. 144 di 300 31/08/2011 Stacchiotti A, Bonomini F, Lavazza° A, Rodella LF, Rezzania R
Adverse effects of cyclosporine A on HSP25, alpha B-crystallin and myofibrillar cytoskeleton in rat
heart
Toxicol. - Vol. 262 ( 2009). - p 192-198. - bib ref 51
Impact factor: 2,836
Small heat shock proteins (sHSPs), such as HSP25 and alpha B-crystallin, are cytoprotective stress proteins
exceptionally represented in the heart. They act as myofibrillar chaperones that help actin and desmin to
maintain their optimum configuration and stability, thereby antagonizing oxidative damage. The present study
examined: (1) the cardiac distribution and abundance of HSP25 and alpha B-crystallin in rats receiving CsA at
a therapeutic dosage (15 mg/kg/day) for 42 days and 63 days; (2) the presence of myofibrillar proteins, such
as actin, alpha-actinin and desmin following the CsA treatments; (3) the subcellular effects of prolonged CsA
exposure on the cardiomyocytes by histopathology and transmission electron microscopy. After 63 days CsA
intake, sHSPs translocated from a regular sarcomeric pattern to peripheral sarcolemma and intercalated
discs, together with actin and desmin. In contrast, the sarcomeric alpha-actinin pattern did not change in all
experimental groups. The abundance of actin and HSP25 was unchanged in every time point of treatment
while after 63 days CsA, alpha B-crystallin and desmin levels significantly decreased. Furthermore CsA
induced fibrosis, irregular sarcomeric alignment and damaged desmosomes. These findings indicate that
following prolonged CsA exposure, the cardiac muscle network was affected. In particular, the translocation of
sHSPs to intercalated discs merits special consideration as a direct compensatory mechanism to limit CsA
cardiotoxicity.
ID nr. 4120
Stacchiotti A, Morandini F, Bettoni F, Schena I, Lavazza° A, Grigolato PG, Apostoli P,
Rezzani R, Aleo MF
Stress proteins and oxidative damage in a renal derived cell line exposed to inorganic mercury and
lead
Toxicol. - Vol. 264 ( 2009). - p 215-224. - bib ref 63
Impact factor: 2,836
A close link between stress protein up-regulation and oxidative damage may provide a novel therapeutic tool
to counteract nephrotoxicity induced by toxic metals in the human population, mainly in children, of
industrialized countries. Here we analysed the time course of the expression of several heat shock proteins,
glucose-regulated proteins and metallothioneins in a rat proximal tubular cell line (NRK-52E) exposed to
subcytotoxic doses of inorganic mercury and lead. Concomitantly, we used morphological and biochemical
methods to evaluate metal-induced cytotoxicity and oxidative damage. In particular, as biochemical indicators
of oxidative stress we detected reactive oxygen species (ROS) and nitrogen species (RNS), total glutathione
(GSH) and glutathione-S-transferase (GST) activity. Our results clearly demonstrated that mercury increases
ROS and RNS levels and the expressions of Hsp25 and inducible Hsp72. These findings are corroborated by
evident mitochondrial damage, apoptosis or necrosis. By contrast, lead is unable to up-regulate Hsp72 but
enhances Grp78 and activates nuclear Hsp25 translocation. Furthermore, lead causes endoplasmic reticulum
(ER) stress, vacuolation and nucleolar segregation. Lastly, both metals stimulate the over-expression of MTs,
but with a different time course. In conclusion, in NRK-52E cell line the stress response is an early and metalinduced event that correlates well with the direct oxidative damage induced by mercury. Indeed, different
chaperones are involved in the specific nephrotoxic mechanism of these environmental pollutants and work
together for cell survival.
ID nr. 4121
Rugna° G, Merialdi° G, Bardasi° L, Bassi° S, Dell'Anna° S, Fontana° MC, Galletti°G,
Massi° P, Santi A, Tamba° M
Survey on Campylobacter spp. prevalence in broiler chickens slaughtered in Emilia-Romagna
Pag. 145 di 300 31/08/2011 Region
XIX Convegno Nazionale AIVI : la filiera corta: lo stato dell’arte, la tutela del consumatore, le difficoltà e le
prospettive : Perugia 24 - 25 - 26 Giugno 2009 / [s.n. : s.l., 2009]. - p 39
Convegno Nazionale AIVI (19 : Perugia : 24 - 25 - 26 Giugno 2009)
First author Rugna, Gianluca
Thermophilic Campylobacter spp. bave been recognised as a major cause of foodborne infections in many
countrics throughout the world. Poultry meat is the most common source for foodborne cases of human
campylobacteriosis. An European baseline study (Dec. 516/07/UE) was carried out in the ycar 2008 with the
aim ol dctcrmining the prevalence of Campylobacter spp. in broiler chickens and the contamination Ievel on
the broiler carcasses. One hundred broiler tlocks were sampled in 4 poultry slaughterhouses in Emilia
Romagna and 52% (IC 95`Y,: 41,8`%-62,1`%,) were positive for Campylobacter jejuni/coli. The prevalence of
thermophylic Campylobacter on carcasses was 26,0%) (IC 95`%0: 17,7'%0-35,7`%,) and it was correlatcd to
finding of these bacteria in the broilers' gut (O.R.: 3,8; I.C. 95(/,: 1,4-9,9).
ID nr. 4122
Stacchiotti A, Li_Volti G, Lavazza° A, Rezzani R, Rodella LF,
Schisandrin B stimulates a cytoprotective response in rat liver exposed to mercuric chloride
Food Chem Toxicol. - Vol. 47 ( 2009). - p 2834-2840. - 50 bib ref
Impact factor: 2,321
Mercury represents an ubiquitous environmental toxic metal. Heat shock proteins (HSP) and metallothioneins
(MTs) help to protect cells against metal toxicity. Schisandrin B (Sch B), a lignoid from Schisandra chinensis,
has been successfully used to treat hepatitis, but its effect against mercury hepatotoxicity remains unknown.
We analyzed whether Sch B could protect rat liver against mercuric chloride (HgCl2) intake by analyzing
stress proteins and histopathological changes.Wistar rats were administered Sch B (10 mg/kg/day by gavage)
or vehicle (olive oil) for 10 days. A subset of each group also received low-dose HgCl2 (0.1 mg/kg/day) for 3
days on days 8–10. Another group received Sch B for 10 days with a single high dose of HgCl2 (1 mg/kg
intraperitoneally) on day 10. In rats treated with Sch B and HgCl2, HSP72, HSP25 and MTs were
overexpressed in liver zones 1 and 3 irrespective of HgCl2 dosing schedules. Furthermore Sch B alone
induced perinuclear rough endoplasmic reticulum alignment and if associated to HgCl2, increased
mitochondrial density and dense bodies, all signs of intense detoxification machinery. Taking together these
data suggest that dietary Sch B counteracts HgCl2 hepatotoxicity in the rat by stimulating chaperones
responsible for anabolic activity.
ID nr. 4123
Boniotti° MB, Goria M, Loda° D, Garrone A, Benedetto A, Mondo A, Tisato E, Zanoni° M,
Zoppi S, Dondo A, Tagliabue° S, Bonora S, Zanardi° G, Pacciarini° L
Molecular typing of mycobacterium bovis strains isolated in Italy from 2000 to 2006 and evaluation of
variable-number tandem repeats for geographically optimized genotyping
J Clin Microbiol. - Vol. 47 no 3 ( 2009). - p 636-644. - 36 bib ref
Impact factor: 3,945
Project PRC2003014 - Planning and development of a M. bovis genotype data base to support
epidemiological investigations of TB outbreaks. - Pacciarini Dr.ssa Maria - Capofila - MINSAN 2003 - 98500,00 EUR - 26/04/2004 0.00.00 - 25/08/2007 0.00.00 - 1 - H2 - Reparto di Biologia
Molecolare - Pacciarini Dr.ssa Maria - I
2 - G2 - Reparto di Batteriologia Specializzata - Tagliabue Dr.ssa Silvia - I
3 - T1 - OEVRL - Zanardi Dr. Giorgio - I
4 - IZSPLV - Goria Dr.ssa Maria - E
First author Boniotti, Beatrice
Pag. 146 di 300 31/08/2011 Spoligotyping and exact tandem repeat (ETR) analysis of Mycobacterium bovis and M. caprae isolated strains
has been routinely carried out in Italy since 2000 to obtain a database of genetic profiles and support
traditional epidemiological investigations. In this study, we characterized 1,503 M. bovis and 57 M. caprae
isolates obtained from 2000 to 2006 in 747 cattle herds mainly located in northern Italy. We identified 81
spoligotypes and 113 ETR profiles, while the combination of spoligotyping/ETR analysis differentiated 228
genotypes, with genotypic diversity indices of 0.70 (spoligotyping), 0.94 (ETR-A to -E typing), and 0.97
(spoligotyping/ETR-A to -E typing), respectively. Despite the high degree of resolution obtained, the
spoligotyping/ETR methods were not discriminative enough in the case of genotypes characterized by the
combination of SB0120, the predominant spoligotype in Italy, with the most common ETR profiles. To obtain a
more informative subset of typing loci, 24 mycobacterial interspersed repetitive unit-variable-number tandem
repeat (MIRU-VNTR) markers were evaluated by analyzing a panel of 100 epidemiologically unrelated
SB0120 isolates. The panel was differentiated into 89 profiles with an overall genotypic diversity of 0.987 that
could be also achieved by using a minimal group of 13 loci: ETR-A, -B, and -E; MIRU 26 and 40; and VNTR
2163a, 2163b, 3155, 1612, 4052, 1895, 3232, and 3336. The allelic diversity index and the stability of single
loci was evaluated to provide the most discriminative genotyping method for locally prevalent strains.
ID nr. 4124
Rota_Nodari° S, Lavazza° A, Candotti° P
Technical note: rabbit welfare during electrical stunning and slaughter at a commercial abattoir
World Rabbit Sci. - Vol. 17 no 3 ( 2009). - p 163-167. - 10 bib ref
Impact factor: 0,863
First author Rota_Nodari, Sara
A total of 1020 crossbreed rabbits were individually examined to evaluate their welfare during electric stunning
and slaughter in a commercial abattoir. Stunning (the position of electrodes and repetition of applications of
current) and sticking (the position, length and depth of the cut) procedures were checked. The rabbits were
monitored behaviourally from the application of the current to death. The stunning system was incorrectly
applied one hundred and ten times (10.8%). Three rabbits failed to be stunned and were still conscious at
sticking. Eighteen rabbits recovered before the onset of death, as shown by their corneal reflex and in a few
cases, vocalization (n=3) and head movement (n=1) were observed. Corneal reflex seemed to be the best
indicator of recovery at the abattoir.
ID nr. 4125
Bellini° S, Alborali° L, Massirio I, Cinotti° S
Biosecurity practices, pig-farming system, farm risk level, health status certification
Large Anim Rev. - Vol. 15 no 5 ( 2009). - p 205-210. - 8 bib ref
First author Bellini, Silvia
In Italy pig industry is one of the most important sectors in livestock husbandry, also as a consequence of the
economic value of by-product production. Pig farming is mainly concentrated in Northern Regions where, over
the past decade a significant increase in pig population has occurred. In the remaining Regions pig farming is
less specialized and it is characterized by small-scale holdings mainly for self-consumption or for small-scale
trade. The presence of different pig farming systems determines the coexistence of different: management
systems, production scales, health and bio-security standards, market requirements and production costs; all
these factors are considered relevant for diseases spreading. Theoretically the application of rigorous
prevention measures (bio-security) may modulate the risk of disease diffusion but, data emerged during the
surveillance and eradication campaigns, indicate that the bio-security measures applied are too weak to
guarantee the status of the entire system. This inconvenience could be overcome throughout the assignment
of different levels of risk, to be established throughout the collection of objective parameters on the holding.
Trade between farms of the same level of risk and sure health standard could guarantee the status of the
Pag. 147 di 300 31/08/2011 entire sector and safeguard the economic and market requirements of the different production systems.
ID nr. 4127
Matassa R, Vinco° LJ, Diegoli G, Montella L
Veterinary training course in farm animal welfare in accordance with EEC 98/58
World Poultry Sci J. - Vol. 2009). - p 54
European Symposium on Poultry Welfare (8th : Cervia, Italy : 18-22 May 2009)
Impact factor: 1,398
Veterinary training courses in farm animal welfare in accordance with EEC 98/58 Animal welfare legislation
are undergoing continuous changes also due to political decisions often supported by public opinion. The
application of new welfare laws however often require adjustments as they are applied in the field especially
when dealing with farm animals. In this case legislation application often may influence decisions taken by
operators that are very expert in one particular area. This fact may have a deep impact with severe economic
consequences on very advanced production systems such as the poultry industry. Poultry production, as a
matter of fact, is carried out by highly specialised and huge integrated companies. The Italian government
veterinary services are, on the other hand, organised in local health units subdivided in functional areas (A=
Live animals. B= Slaughtered animals and C= Other, including animal welfare). This type of organization
ensures an accurate veterinary control of the territory, but cannot ensure that all the vets are highly
specialised in each production sector. For this reason very often the official veterinarian is not capable to deal
with the poultry operators by giving them the right answers and making the right decisions. For this specific
reason the general management of animal welfare and veterinary products of the Labour Ministry, of the
Health Ministry and Social Policies have financed a training programme on the subject of animal welfare, with
the aim of informing and training animal farmers. Each event lasted 3 days. The first morning session was
dedicated to general legislation involving all animal farm species. The following sessions were dedicated to
specific species. Field private vets have been recruited as speakers in order to ensure the provision of
updated data thus providing adequate instruments to adapt legislation to specific field situations. Regarding
poultry the following species were treated: broilers, turkeys, layers and minor species.
ID nr. 4128
Petracci M, Amadori° M, Archetti° IL, Bianchi M, Montella L, Cavani C
Effect of feeding during long transport on welfare of laying pullets
World Poultry Sci J. - Vol. 2009). - p 102
European Symposium on Poultry Welfare (8th : Cervia, Italy : 18-22 May 2009)
Impact factor: 1,398
A study was conducted to test the effects of a newly-developed transport feed (TF) with high water content on
pullets transported more than 12 hours. After preliminary tests on farmed pullets, three individual transport
trials were conducted, using a total of 330 animals (Lohmann Brown, 16-wk-old, average body weight: 1.384
kg), transported in 48×54×21 cm (length×width×height), metal wire commercial crates, divided into 2 groups.
Control pullets were kept without feed and water and loaded at the usual commercial stocking density (8
pullets/crate; 324 cm²/animal), whereas the experimental group had access to TF and were loaded at the
same density considering TF surface (7 pullets/crate; 324 cm²/animal). Birds were placed on commercial
lorries and transported for 20 hours. Before catching, and at the end of transport, blood samples (15
animals/group at random) were taken from vena ulnaris superficialis and used to determine hematocrit, total
protein, sodium, glucose, plasma corticosterone, heterophil/lymphocyte (H/L) ratio, reactive oxygen
metabolites (ROMs), total antioxidant power (OXY-TA) and lisozyme. TF consumption as well as changes in
body weight and body (cloacal) temperature were also assessed. Mean TF consumption in experimental
groups was 67.0 g/animal (12.5 g dry matter and 54.5 g water) with an energy supply of 0.206 MJ/animal.
Pullets that had access to FT had lower body weight losses (6.4 vs. 5.2%; P<0.01) and a lower decrease of
body temperature (-0.1 vs. -0.3°C; P<0.05), compared with controls. Moreover, TF pullets showed significantly
Pag. 148 di 300 31/08/2011 lower plasma corticosterone, lisozyme, sodium and OXY-TA values as well as a reduced
heterophil/lymphocyte ratio. Overall, these findings indicate that TF can reduce negative energy balance and
stress in pullets during long-distance journeys.
ID nr. 4129
Carra° E, Taddei R, Barbieri° I, Botti° G, Tranquillo° V, Iori° A, Gibelli° L, Cerioli° M,
Cavadini° P, Gelmetti° D, Pongolini° S, Capucci° L
Evaluation of three rapid diagnostic tests used in bovine spongiform encephalopathy monitoring in
Italy
J Vet Diagn Investig. - Vol. 21 no 6 ( 2009). - p 830-836. - 16 bib ref
Impact factor: 1,403
First author Carra, Elena
In 2001, a compulsory active surveillance system was started in the European Union to assess the prevalence
of bovine spongiform encephalopathy (BSE) in the cattle population. The aim of the current study was to
report on the field performances of 3 rapid tests: a Western blot (WB), a chemiluminescence enzyme-linked
immunosorbent assay (ELISA), and an immunochromatographic assay, routinely used at 3 laboratories of the
Istituto Zooprofilattico Sperimentale of Lombardia and Emilia Romagna, over 8 years of BSE monitoring
activity. A total of 2,802,866 samples from slaughtered animals and 202,453 samples from fallen stock were
tested by 1 of 3 tests. Positive results of the rapid tests were confirmed by histopathological examination,
immunohistochemistry, and confirmatory WB. The field performances (i.e., initial reactive and false-positive
rates) and practical aspects regarding resources and applicability of the tests to high-throughput routine
testing laboratories were evaluated. The 3 tests proved to be reliable tools when applied to slaughtered
samples, showing no or very low false-positive rates (<1 per 100,000 negative samples tested) and low
retesting frequencies (0.02–0.26%). When samples from fallen stock were analyzed, performances of the
immunochromatographic assay, and especially the chemiluminescence ELISA, were negatively affected,
resulting in higher false-positive and retesting rates. On the other hand, both tests are less expensive, much
easier to use, provide more rapid results, and adapt well to application in routine laboratories as compared
with WB. In the authors' experience, the immunochromatographic assay was a good compromise between
performance and convenience.
ID nr. 4130
Pisoni G, Zadoks RN, Vimercati C, Locatelli C, Zanoni° MG, Moroni P
Epidemiological investigation of Streptococcus equi subspecies zooepidemicus involved in clinical
mastitis in dairy goats
J Dairy Sci. - Vol. 92 no 3 ( 2009). - p 943-951. - 27 bib ref
Impact factor: 2,486
An outbreak of clinical mastitis was observed in dairy goats due to the zoonotic pathogen Streptococcus equi
ssp. zooepidemicus. Affected goats were culled to prevent transmission of infection to other animals or
humans. The objective of the study was to determine whether horses on the same farm were the source of
the pathogen. Streptococcus equi ssp. zooepidemicus was obtained from milk of 10% of goats in the herd and
from feces of 3 of 7 healthy horses that shared pasture and housing with the goats. Isolates of caprine and
equine origin had identical biochemical profiles, including the ability to ferment sorbitol and lactose, which
distinguishes S. equi ssp. zooepidemicus from S. equi ssp. equi. Sequencing of the 16S–23S intergenic
spacer region and results from sodA-seeI multiplex PCR supported identification of isolates as S. equi ssp.
zooepidemicus. Based on random amplified polymorphic DNA typing and rpoB and sodA sequencing, caprine
isolates were indistinguishable from each other, but distinct from equine isolates. Further analysis of equine
fecal samples showed that multiple strains of S. equi ssp. zooepidemicus can be present in a single sample or
in sequential samples obtained from a single horse. Failure to detect the mastitis-causing strain in equine
feces may indicate that horses were not the source of the mastitis outbreak in goats. Alternatively, the
Pag. 149 di 300 31/08/2011 outbreak may be due to presence of multiple S. equi ssp. zooepidemicus strains in equine feces and a failure
to detect all strains when analyzing a limited number of isolates per sample.
ID nr. 4131
Bellini° S, Cordioli° P, Cinotti° S
Swine Vesicular Disease and weakness detected in swine production system
Summa anim reddito. - Vol. 4 no 3 ( 2009). - p 31-35. - 9 bib ref
First author Bellini, Silvia
Swine Vesicular Disease (SVD) is an infectious disease of pigs induced by an Enterovirus of the
Picomaviridae family. SVD does not cause severe production losses but it is of major economic importance
since control and eradication measures are costly, and nations which are known to have the disease face
embargoes on the export of pigs and by products. In Europe in the last decade, SVD has been persistently
reported in Italy and for this reason surveillance and eradication activities are in place. In the last 3 years SVD
outbreaks have been persistently reported in the non qualified regions but severe outbreaks occurred also in
central and Northern Italy. In fact, in 2006 and 2007 the disease was detected in Lombardia while in 2008 the
disease spread widely in Umbria (Perugia province). The epidemic in northern areas was characterized by a
rapid spread of the condition and difficulties were encountered in eradicating the disease. The paper
examines the factors involved in the impairment of the national eradication programme, which are linked to: 1)
farm census 2) registration of animal movements 3) qualification of the holding and type of control performed
4) farro biosecurity standard. Suggestions are given to overcome the problems and for the qualification of the
holding according to a " level of risU, to be established throughout the collection of objective parameters on
the holding.
ID nr. 4132
Tamba° M, Dell'Anna° S, Luppi° A, Bonilauri° P, Rugna° G, Dottori° M, Cordioli° P
West Nile Disease: an update
Prax Vet. - Vol. 30 no 2 ( 2009). - p 5-9. - 12 bib ref
First author Tamba, Marco
West Nile virus (WNV) is a mosquito transmitted flavivirus. It is widely distributed in Africa the Middle East,
southern Europe, and Asia and was recently introduced Io North America. Birds are involved in the cycle of
transmission as amplifying hosts, but WNV can infect many species of mammals, as well as amphibians and
reptiles. Humans and horses are considered accidental dead-end hosts. but transmission through blood
donations, organ transplants, and the intrauterine route have been reported. In Europe, an enhanced
surveillance of WN infection in humans, horses, birds, and vectors mav reveal the presence of the virus in
different locations. However, very little is known of the ecology and natural history of WN virus transmission in
Europe and most WN outbreaks in humans and animals remain unpredictable and difficult to control.
ID nr. 4133
Magnino° S, Vicari° N, Boldini° M, Rosignoli° C, Nigrelli° A, Andreoli° G, Pajoro° M, Fabbi°
M
Detection of Coxiella burnetii in bulk milk of dairy cattle from Lombardy, Italy
Large Anim Rev. - Vol. 15 no 1 ( 2009). - p 3-6. - 1 bib ref
First author Magnino, Simone
We carried out a survey in order to detect Coxiella burnetii, the agent of Q fever, in cows’ bulk tank milk (BTM)
Pag. 150 di 300 31/08/2011 sampled in 2007-2008 in 400 dairy herds of three provinces of Northern Italy (Cremona, Mantova, Pavia).
With this aim, we employed a PCR assay targeting a segment of the transposon-like genomic region. Overall,
160 BTM samples out of 400 (i.e. 40%) tested positive. We then collected a further BTM sample from several
C. burnetii positive herds as well as from C.burnetii-negative herds, for confirming or excluding the result of
the first assay. The second sample tested positive and negative, respectively, in about 62 % of positive herds
and in about 70% of negative herds. The detection of C. burnetii in cows’ milk has been already documented
in the past. The occurrence of the organism in BTM depends on the number of infected lactating cows and on
the presence of C. burnetii super-shedders in the herd. An increase or decrease in the number of milkshedder cows will accordingly affect the result of the BTM testing. The effects of the infection on the fertility of
cows, as well as the implications for the health of professionally and accidentally exposed humans should be
investigated
.
ID nr. 4134
Luini° M, Benedetti° V, Piccinini R, Vezzoli° F
Campylobacter jejuni mammary infection in cattle: case report
Large Anim Rev. - Vol. 15 no 2 ( 2009). - p 51-54. - 26 bib ref
First author Luini, Mario
The contamination of bulk milk from pathogenic microorganisms such as Campylobacter jejuni is assuming
more importance as a consequence of the increasing consumption of raw milk. We describe two cases of
dairy farms located in Po Valley, characterised by repeated detection of C. jejuni in bulk milk. CASES - In
both cases the source of contamination of bulk milk (and consequently of raw milk) was a single udder quarter
tinfection of one cow.. Just one infected quarter was able to contaminate the bulk milk of respectively 270 and
180 lactating cows. A persistent infection of the quarter was documented for at least 90 days in one herd, for
30-40 days in the other herd . Antibiotic treatment of the infected cow in the first farm was effective in the cure
of mammary infection. DISCUSSION AND CONCLUSIONS - Although C.. jejuni was also detected in the
faeces of some animals, the segregation of the subject with mammary infection was effective in eliminating
the primary source of contamination, with the result of safe bulk milk. The analysis of pooled milk samples by
culture method and PCR, followed by individual testing, are efficient tools for rapid identification of infected
subjects. These data demonstrate the importance to consider the possibility of udder infection of even a single
cow, as a source of bulk milk contamination, when C.. ..jejuni is repeatedly detected..
ID nr. 4135
Amadori° M, Farinacci M, Begni° B, Faita° R, Podavini° D, Colitti M
Effects of interferon-[alfa] on the inflammatory response of swine peripheral blood mononuclear
cells
J Interferon Cytokine Res. - Vol. 29 no 4 ( 2009). - p 241-247. - 35 bib ref
Impact factor: 1,774
Project PRC2005002 - Assessment and modulation of the inflammatory effects of early weaning in the piglet
- Amadori Dr. Massimo - Solo - MINSAN - 2005 - 22000,00 EUR - 15/05/2006 0.00.00 14/11/2008 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
First author Amadori, Massimo
Interferon-a (IFN-a) at low concentrations had been previously shown to control the expression of
inflammatory cytokine genes in swine pulmonary alveolar macrophages. In the first part of this study, cultured
swine peripheral blood mononuclear cells (PBMCs) were supplemented with IFN-a at low/moderate
concentrations, and then stimulated with lipopolysaccharide (LPS). The expression of IFN-a, IFN- , IL-1ß,
TNF-a, and IL-6 genes was determined by real-time PCR. IFN-a at low/moderate concentrations did not
significantly reduce the expression of any cytokine gene under study, with clear trends though to a
Pag. 151 di 300 31/08/2011 concentration-dependent reduction of IL-1ß gene expression and to a concentration-dependent increase of
IFN- gene expression. In vivo, orally administered IFN-a was shown instead to modulate the inflammatory
response to early weaning in uncultured PBMCs of specific pathogen-free piglets. As opposed to the in vitro
model, the oral IFN-a treatment reduced after weaning the expression of the IFN- gene (P < 0.08) and
increased that of the IL-1ß gene (P < 0.05). There was also a trend to a reduced expression of both IL-6 and
TNF-a. The above modulation of cytokine genes expression and the greater daily mean weight gain of treated
piglets highlight important regulatory properties of oral IFN-a in the response to the weaning stress.
ID nr. 4136
Moreno° A, Brocchi° E, Lelli° D, Gamba° D, Tranquillo° M, Cordioli° P
Monoclonal antibody based ELISA tests to detect antibodies against neuraminidase subtypes 1, 2
and 3 of avian influenza viruses in avian sera
Vaccine. - Vol. 27 no 36 ( 2009). - p 4967-4974. - 25 bib ref
Impact factor: 3,298
First author Moreno_Martin, Ana
The objective of this study was the development and the evaluation of competitive ELISA assays based on
monoclonal antibodies for the detection of antibodies specific for neuraminidase type 1 (N1), 2 (N2) and 3
(N3) in avian sera. A total of 1450 sera from different avian species (854 negative, 185 positive to N1, 136
positive to N2, 219 positive to N3 and 56 positive to other N subtypes sera) were analysed in parallel by the
three ELISAs. ROC analyses were performed to enable the selection of best cut-off values and estimation of
diagnostic specificity and sensitivity. In addition, the correlation between the new developed ELISAs and the
neuraminidase inhibition test was evaluated on a limited number of sera. The validation process of the three
ELISAs proved excellent diagnostic performances, with very high specificity and sensitivity, ranging from 99.4
to 99.8% and from 97.6 to 100%, respectively in the three assays. The discriminating potential between
antibodies elicited against homologous and heterologous N validates the test for use in “DIVA” assays, to
distinguish between vaccinated and infected birds.
ID nr. 4137
Zecconi A, Piccinini R, Fiorina S, Cabrini L, Daprà V, Amadori° M
Evaluation of interleukin-2 treatment for prevention of intramammary infections in cows after calving
Comp Immunol Microbiol Infect Dis. - Vol. 2009). - p 439-451. - 27 ref bib
Impact factor: 1,607
A low-dose treatment based on interleukin-2 (IL-2) was investigated for preventing mastitis in dairy cows. The
treatment consisted of a single dose of IL-2 injected into the skin region drained by the supramammary lymph
node 3–5 days after calving. The study included 45 cows (23 treated and 22 controls) from three commercial
dairy herds. The results showed that the treatment had no side effects. The treatment with IL-2 induced the
significant increase of several milk markers related to leukocyte and epithelial cell functions, i.e. SCC (somatic
cell counts), serum amyloid A (SAA), lactoferrin and NAGase. The increased concentration of milk markers
suggested also an activity of IL-2 on epithelial cells, resulting in a higher resistance to invading pathogens.
Indeed, the increased efficiency of cells in the udder is supported by the higher frequency of healthy quarters
observed in the treated group until day 17–19 after calving, in comparison with the control one.
ID nr. 4138
Petrini S, Barocci S, Gavaudan S, Villa° R, Briscolini S, Sabbatini M, Mattozzi C, Barchiesi
F, Salamida S, Ferrari° M, Paniccià M, Pezzotti G
Detection of porcine circovirus type 2 (PCV2) from wild boars in central Italy
Pag. 152 di 300 31/08/2011 Eur J Wild Res. - Vol. 55 no 5 ( 2009). - p 465-469. - 18 bib ref
Impact factor: 1,543
The lesions observed in 16 wild boars, hunted in central Italy, led to the suspect that could be related to the
infection by porcine circovirus type 2 (PCV2). The animals had macroscopic and histological lesions in the
lungs, tonsils, and bronchial lymph nodes. PCV2 was detected in tissue samples by polymerase chain
reaction (PCR) and immunohistochemistry and it was isolated in newborn swine kidney cell cultures. From the
infected cell culture supernatant, the presence of PCV2 DNA was confirmed by real-time PCR whereas virus
particles were observed by electron microscopy. These diagnostic data indicate that PCV2 can infect and
cause disease in Sus scrofa subspecies other than domestic swine and it is present in the wild boar
population in central Italy.
ID nr. 4139
Bertocchi° L
Milk cow and welfare law , rules and future development in Europe
Inf Zootec. - Vol. 56 no 16 ( 2009). - p 48-51
First author Bertocchi, Luigi
Since a long time it seems necessary to rule, as the same of calf farming that is ruled yet, the grown-up dairy
cow. The legislation must take into account the animal natural behaviour to meet physiological needs and
respect welfare. At the moment, the lower is writing the eighth proof that underlines the relevance of
management, housing the education of caw herd.
ID nr. 4140
Decaro N, Campolo M, Mari V, Desario C, Colaianni ML, Di_Trani L, Cordioli° P,
Buonavoglia C
A candidate modified-live bovine coronavirus vaccine: safety an immunogenicity evaluation
New microbiol. - Vol. 32 ( 2009). - p 109-113. - 14 bib ref
Impact factor: 0,912
Project PRF2007202 - Mammal coronaviruses: molecular epidemiology, vaccine development And
implications for animal and human health - Cordioli Dr. Paolo - Capofila - MINSAN - 2007 300000,00 EUR - 01/09/2009 0.00.00 - 31/08/2011 0.00.00 - 1 - U1 - REPARTO VIROLOGIA Cordioli Dr. Paolo - I
2 - Università di Bari - Dipartimento di Sanità Pubblica e Zootecnica - Decaro Prof. Nicola - E
3 - IstitutoZooprofilattico della Sardegna - Oggiano Dr.ssa Annalisa - E
4 - Istituto Zooprofilattico della Sicilia - Guercio Dr.ssa Annalisa - E
5 - Istituo Zooprofilattico delle Venezie - Nardelli Dr. Stefano - E
A modified-live vaccine against the respiratory form of bovine coronavirus (BCoV) infection was developed by
progressive attenuation of a respiratory strain (438/06-TN). The vaccine was found to be safe as four
colostrum-deprived newborn calves remained healthy after oronasal administration of ten doses of the
vaccine. The immunogenicity of the vaccine was assessed by intramuscular injection of one vaccine dose to
30 BCoV-antibody negative 2-3-month-old calves. At 30 days post-vaccination, all vaccinated calves
displayed high antibody titres against BCoV. Sequence analysis of the S gene of wild-type and cell-adapted
438/06-TN strain detected 10 nucleotide changes, 9 of which were nonsynonymous.
ID nr. 4141
Rossi L, Galante F, Fusi E, Luini° M, Dell'Orto V, Baldi A
Pag. 153 di 300 31/08/2011 Evaluation of the PL-PG-PA system in relation to quality of bovine milk
Vet Res Commun. - Vol. 33 suppl 1 ( 2009). - p 293-295. - 6 bib ref
Impact factor: 0,630
ID nr. 4142
Finazzi° G, Daminelli° P, Ducoli° S, Boni° P, Cosciani_Cunico° E
Botulinum toxin inactivation during cooking of artificially contaminated potato gnocchi
Ind Aliment (Pinerolo). - Vol. 48 ( 2009). - p 38-41. - 8 bib ref
Project RELOPATABIODEC - Bio-decontanimation tools and methods applied to food firms - Boni Dr. Paolo
- Capofila - REGIONE - 2007 - 285064,92 EUR - 01/10/2007 0.00.00 - 30/09/2010 0.00.00 - 1 I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni Dr.
Paolo - I
2 - Gruppo Impresa Finance srl - Tabladini Dr. Marco - E
First author Finazzi, Guido
Potato gnocchi are usuolly sold in pockoge reporting the instructions for consumere "cook in boiling salted
water unti) rising to the surfoce". The object of this trial was to verify if this treatment is sufficient io inoctivote
botulinum toxin in case of gnocchi accidentally contaminated with CI. botulinum. Four different types of
gnocchi produced by the some foctory were contaminated with botulinum toxin obtoined from o broth medium
of four different CI. botulinum strains. Each type of gnocchi was put in o pan of boiling salted water and
different portions were token at floating and also ofter one minute from floating. The botulinum toxin, using o
biologicol test conducted on mice, was not found in ony of analysed somples. Therefore the potential
possibility of the presence of botulinum toxin in gnocchi remains remote, but it does not represent a risk for
consumers who cook this type of product properly.
ID nr. 4146
Riu R, Liuzzo G, Merialdi° G, Bardasi° L, Galletti°G, Coccollone A, Giacometti F, Serraino
A, Rosmini R
Relationship between level of contamination on hide of animals presented for slaughter and
contamination on the resultant carcasses
XIX Convegno Nazionale AIVI : la filiera corta: lo stato dell’arte, la tutela del consumatore, le difficoltà e le
prospettive : Perugia 24 - 25 - 26 Giugno 2009 / [s.n. : s.l., 2009]. - p 44
Convegno Nazionale AIVI (19 : Perugia : 24 - 25 - 26 Giugno 2009)
The hide and viscera of cattle entering a slaughter facility are potential sources of contamination of carcasses
including significant pathogens such as Escherichia coli O 157: H7, Campylobacter spp. and Salmonella spp.
If evisceration is correctly performed, visceral contents do not therefore contribute significandy to the overall
contamination of the carcass. However, it is much more difficult to restrict contamination from the hide. The
aim of this work is to investigate the relationships between the levels of dirt and contamination on the hide of
animals presented for slaughter and the levels of contamination on the resultant carcasses. Cattle were
visually inspected in the lairage of 2 abattoirs and assigned to a category ranging from 1 (very clean) to 5
(very dirty) depending on the observed cleanliness of the hide. Samples of hides and carcasses were taken
from 50 animals for enumerate total viable counts, Enterobacteriaceae and Escherichia coli. The results show
that there is a direct correlation between animai dirtiness and total bacterial count of the carcasses.
ID nr. 4147
Moreno° A, Lelli° D, Barbieri° I, Canelli° E, Tamba° M, Avisani° D, Bonilauri P, Cordioli° P
Diagnostic approach to a West Nile virus outbreak in Northern Italy on fall 2008
Pag. 154 di 300 31/08/2011 Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey / [s.l. : s. n., 2009]. - 1
p. - 3 bib ref
Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
Project EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Moreno_Martin, Ana
ID nr. 4148
Amadori° M, Farinacci M, Colitti M
Low-dose interferon-alpha treatment can modulate the stress of early weaning in pigs
Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey / [s.l. : s. n., 2009]. - 1
p. - 3 bib ref
Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
First author Amadori, Massimo
Interferon-alpha (IFN-alpha) at low concentrations had been previously shown to control in vitro the
expression of inflammatory cytokine genes in swine pulmonary alveolar macrophages. Owing to the above,
we wondered if a similar control action could be exerted by IFN-alpha in piglets during a natural stressing
event like early weaning, characterized by a high proflogistic potential. To this purpose, four Specific
Pathogen Free (SPF) littermate piglets were given human lyrnphoblastoid interferon-alpha for io days in a row
in form of a freeze-dried preparation, mixed in the diet, starting on the day of early weaning at 21 days of age.
The other four littermate piglets served as untreated controls; they received the same daily mass of placebo in
a separate weaning cage. The expression of IFN-alpha, IFN-gamma, IL-1 beta, TNF-alpha and IL-6 genes
was determined in uncultured PBMC by real time PCR. Orally administered IFN-alpha was shown to reduce
alter weaning the expression of the IFN-gamma gene (p<o.o8) and to increase that of the IL-i beta gene
(p<o.o5). There was also a trend to a reduced expression of both IL-6 and TNF-alpha genes. Relatively to the
day of weaning, endogenous IFN-alpha was demonstrated in sera and also PBMC of untreated, control
piglets at day +3, as opposed to day -1. The above modulation of cytokine gene expression and the greater
daily mean weight gain of IFN-treated piglets highlight important regulatory properties of ora] IFN-alpha
treatment at weaning, which probably resemble those of the endogenous cytokine. A greater daily mean
weight gain and significant changes in the time-course of the main inflammatory cytokines in sera were also
shown in a field trial of oral, low-dose IFN-alpha treatment at weaning.
ID nr. 4149
Moreno° A, Sozzi° E, Lelli° D, Vinco° LJ, Lombardi° G
Experimental infections of pigs with H7 and H5 avian influenza viruses
Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey / [s.l. : s. n., 2009]. - p
199. - 3 bib ref
Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
Project EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Moreno_Martin, Ana
ID nr. 4150
Pezzoni° G, Stercoli° L, Cordioli° P, Brocchi° E
Serodiagnosis of pestiviruses infection by competitive-ELISA based on monoclonal. antibodies and
recombinant ns3
Pag. 155 di 300 31/08/2011 Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey : Programme and
Abstract / [s.l. : s. n., 2009]. Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
Project PRC2005012 - Production and characterization of recombinant viral proteins as antigens for
serology - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2005 - 68000,00 EUR - 04/09/2006 0.00.00
- 03/09/2009 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Pacciarini Dr.ssa Maria - I
First author Pezzoni, Giulia
Bovine Viral Diarrhea Virus (BVDV), Border Disease virus (BDV) and Classical Swine Fever virus (CSFV)
belong to Pestivirus group, the three viruses are antigenically related. The most immunogenic and conserved
protein among pestiviruses is the non strutturai protein 3 (NS3), a multifunctional enzyme with at least two
domains associated with enzymatic activities: a serine protease activity and an NTPase-elicase activity.
Currently, serological assays for Pestiviruses are based on the detection of antibodies against NS3. We
expressed the NS3-NTPase-elicase domain (rNS3E) of BVDV NADL strain in a baculovirus/insect celi
system, in order to preserve the natural antigenic and strutturai properties of the native antigen. The rNS3E is
produced at high yielci and is recognized in immunofluorescence by three anti-NS3 monoclonal antibodies
(MAbs) prevìously raised against an Italian isolate of BVDV. According to the results of reciprocal competition
the MAbs identify three different epitopes on the rNS3E as well as on the native antigen; their absence of
reactivity in Western-blot indicates that the three epitopes are conformation-dependent. When combined as
catching and conjugated antibody in sandwich ELISA assays, the three MAbs showed reactivity profiles with
the rNS3E identical to those observed with.the native antigen. Overall, this reactivity provided evidente that
the recombinant protein reproduces folding and antìgenicity of the native virai protein, providing the conditions
for the development of a functional antibody-detection ELISA for pestiviruses. For the test design we chose a
competitive ELISA, since it is equivalently applicable to any animal species. In particular, the MAb-based
competitive test, routinely used in our laboratory, was modified in order to substitute the BVD virus used as
source of NS3 with the rNS3E. MAb 3H4 was confirmed as the best antigen capture antibody to display most
antigenic epitopes and MAb 3A3, conjugated with peroxidase, as the best competing antibody. Diagnostic
performance of the competitive ELISA based on rNS3E was evaluated by testing bovine and pig sera in
parallel with the in-house test for pestiviruses antibodies; an additional test for CSFV-specific antibodies,
provided by the National Referente Laboratory for CSF (IZS Perugia), was used as confirmatory test far
positive pig seral. Results for 369 bovine sera showed a 100% concordante between the two ELISAs for
pestiviruses, wíth 147 positive and 222 negative samples in both tests. Regarding pig sera, out of 2691 field
samples examined, 221 scored positive and 2456 scored negative in both ELISAs (99.5 % concordant
results). Ali the positive pig sera were elicited by infection with pestiviruses other than CSFV, as none of them
reacted in the CSFV-specific ELISA. The capability of recombinant competitive ELISA to recognize allo
infection by CSF virus was investigated using a panel of sera from pigs experimenta]ly vaccinated with the
CSF China strain and subsequently challenged with a pathogenic virus: results of the immune response
detected by the recombinant ELISA consistently corcelated with results provided by the in-house test based
on the native viral antigen and by the C5FV-specific ELISA. In conclusion, the rNS3E can successfully
substitute the virai antigen in serological diagnosîs of infections cavsed by pestiviruses; the use of a
recombinant antigen in association with characterized MAbs makes the productíon and yield of biological
reagents safer and easier and ensures improved standardization and reproducibility of serological tests.
ID nr. 4151
Calzolari° M, Bonilauri° P, Bellini R, Veronesi R, Pilani R, Defilippo° F, Caimi M, Parco V,
Fedeli P, Barbieri° I, Maioli° G, Lelli° D, Lavazza° A, Cordioli° P, Dottori° M
Arboviral surveillance program on mosquitoes from "valli di Comacchio" and "parco lombardo della
valle del Ticino" (Northern Italy)
Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey : Programme and
Abstract / [s.l. : s. n., 2009]. Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
First author Calzolari, Mattia
Pag. 156 di 300 31/08/2011 Background Recently Italy was involved in two important outbreaks of human arbovirus diseases
(chikungunya and West Nile). In 2oo8 a preliminary surveillance program in two Italian wetlands to check the
presence of arboviruses in mosquitoes was activated. In this abstract the preliminary results of this program
are presented. Methods Mosquitoes were collected with C02 traps in areas with high density of mosquitoes
near Comacchio (Emilia-Romagna region) and in the Ticino River Park (Lombardia region) in the mosquito
season (July-October). Mosquitoes were pooled according to date, location and species. For simultaneous
detection of arbovirus causing most important human and animai disease the pools obtained were tested with
3 screening PCR for the Flavivirus, Alphavirus and Bunyavirus genus. The amplified fragments were
sequenced and analyzed using the Mega 4 program. Virus isolation was carried out on the same pools
analyzeci with PCR by celi culture (Vero and C61C36 cells) and embryonated eggs. Results Wetested a total
of 31.861 mosquitoes (369 pools), 16.156 (203 pools) from Comacchio and 15.705 (166 pools) from the
Ticino River Park. The mosquitoes tested belong to the species Anopheles maculipennis, Aedes vexans, Ae
cinereus, Ae albopictus, Ochlerotatus caspius, O geniculatus, O detrirtus, Culex pipiens and Cx modestus.
The most abundant species were Cx pipiens (43,2%), O caspius (25,9%), Ae vexans (13,3%), An
maculipennis (2,8%). 2 pools of O caspius, captured near Comacchio on July 23rd resulted positive for the
presence of Flavivirus RNA in screening PCR, i pool of 0 caspius captured in Lido di Spina (near Comacchio),
on JUIy 25th and 1 pool of An maculipennis captured in Ticino Park on August 22nd resulted positive in the
Bunyavirus screening PCR. The isolation of viruses produced negative results in alt the samples tested. In
BLAST analysis the sequence of the positive amplified fragment from Ticino Park showed maximum
homology (95%) with Batai virus (GB:AB257762). The sequence of Bunyavirus from Lido di Spina showed
maximum homology (99%) with Marituba virus (GB:AY613923), it also showed a good homology (970/0) with
Tahyna virus (GB:U47142). The 2 pools of O. caspius positive for Flavivirus showed light similarity (less than
8o%) with Cx Flavivirus (CuFV) isolated from Cx pipiens in Japan. Conclusion Bunyavirus positive PCR found
and sequenced in An maculipennis captured in Tirino Park is probably to ascribe to the presence of a Batai
virus, first isolated in 196o from this species of mosquito and not associated with human disease. The
sequence of Bunyavirus obtained form Lido di Spina shows a very high homology with a virus (Marituba virus)
first isolated in Amazon region in Brazil in the 6os. The second hìghest homology obtained in BLAST analysis
is with Tahyna, a virus mainly isolated from Aedes and Ochlerotatus species that caused influenzalike
symptoms in humans in Europe. The isolation of the virus failed, therefore a fina) classificatlon of this positive
PCR was impossible; however the presence of Tahyna virus is more probable than the presence of Marituba
virus in pooled mosquitoes. The detected flaviviruses probably belong to a group of virus that are present only
in mosquitoes species, so this virus does not represent a risk for human and animal populations. These
positive PCR detections demonstrate that a wide range of arboviruses causing human and animal diseases
couid be detected by our surveillance program even in the absence of human or animai disease outbreaks.
ID nr. 4152
Lelli° D, Moreno° A, Sozzi° E, Canelli° E, Tamba° M, Capucci° L, Brocchi° E, Cordioli° P
Serological investigation for Chickungunya virus in different animal species reared in the area of
italian ourbreak in 2007
Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey : Programme and
Abstract / [s.l. : s. n., 2009]. - p 232. - 3 bib ref
Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
First author Lelli, Davide
ID nr. 4153
Massi° P, Tosi° G, Vandi L, Piva A
experimental infection of SPF chickens with a Salmonella hadar field strain and evaluation of the
efficacy of a mixture of organic acids and natural flavors (European patent n. 1391155b1) added to
feed.
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 103-106. - 1 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
First author Massi, Paola
Pag. 157 di 300 31/08/2011 To evaluate the efficacy of a mixture of organic acids and natural flavors added to feed at different
concentrations (0.3, 1.0 and 5.0 kg/T of feed) an experimental infection of SPF chickens with a Salmonella
hadar field strain was carried out. The effects of this treatment on infection and zootechnical parameters are
discussed.
ID nr. 4154
Vida P, Mammone T, Lavazza° A, Moretto° A
European Project “ECORAIP”: European content for public health awareness of rural population on
influenza prevention (2007-2008)
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 121-122
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
The European project “ECORAIP: European content for public health awareness of rural population on avian
influenza prevention (2007—2008)” was founded by the Public Health Executive Agency (PHEA) with
Agreement n. 20067 (http://ecoraip.eu/). Its main objective was to provide the populations living in rural areas
in different European countries with updated and scientific based information and guidelines to prevent and
control the outcome of epidemic outbreaks of influenza, including highly pathogenic avian influenza (H5N1
and similar HPAI viruses).
ID nr. 4155
Massi° P, Tosi° G
An outbreak of Mycoplasma gallisepticum infection in broiler breeders with vertical transmission to
progeny: dynamic of the infection and laboratory investigation
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 101-102. - 2 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
First author Massi, Paola
An outbreak of Mycoplasma gallisepticum in broiler breeders is described. The effects of the vertical
transmission on different broiler flocks are evaluated. The laboratory techniques used for the diagnosis and
their interpretation are discussed.
ID nr. 4156
Taddei° R, Tosi° G, Massi° P
Optimization of a PCR method to detect Salmonella spp. in animal feed
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 116-119. - 3 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
First author Taddei, Roberta
The aim of this study was to optimize a PCR method to detect Salmonella spp. in animal feed. The sensitivity
of three different DNA extraction procedure coupled with a traditional PCR protocol, was evaluated and
compared to the reference cultural method (ISO 6579(2002)/Cor1 (2004)). Sensitivity of the assays was
comparable to the reference cultural method and resulted not related to the extraction protocols tested.
Pag. 158 di 300 31/08/2011 ID nr. 4157
Circella E, Pennelli° D, Tagliabue° S, Ceruti R., Giovanardi D, Camarda A
Geni di virulenza in avian pathogenic Escherichia coli nel tacchino
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 57-62. - 7 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
50 Escherichia coli (APEC-Avian Pathogenic Escherichia coli) strains and 15 E. coli (AFEC-Avian Faecal
Escherichia coli) from turkeys aff ected by colibacillosis and from healthy turkeys were tested for the presence
of eight diff erent virulence-associated genes. Besides, APEC were serotyped. O78 has been the most
detected serotyped. The presence of the tested virulence genes was prevalently related to the APEC isolates.
With reference to serogroup, all the tested O78 resulted iss and irp2 positive. Besides, tsh e cva/cvi were
respectively present in 88.9 and 83.3 % of O78. Nevertheless, the fi nding of a not typeable strains equipped
with all the eight tested
virulence genes among the APEC isolates suggest the importance of a careful and complete characterisation
of the isolate to evaluate the real potential pathogenic attitude of the bacterium.
ID nr. 4158
Colmegna° S, Invernizzi° A, Mascher° A, Corsale E, Ferrazzi V, Grilli G
Microbiological characteristics of poultry meats-results of inspections carried out in the province of
Milan
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 63-68. - 5 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
First author Colmegna, Silvia
Under the qualitative/quantitative microbiological profile (C.B.T., Coliforms,
E. coli, S. aureus, Sulphate-reducing Clostrides, B. cereus, Salmonella spp. and
Lysteria spp. and Campylobacter spp.), 240 samples of poultry meat (chicken, turkey and quail) were
examined and officially considered according to the norms envisioned by the regional plan of the
programming and co-ordinating of operations concerning official inspections of Lombardy animal origin and
from a few private industries for self-inspection. The CBT always turned out to be less and in line with that
reported in the bibliography, as is also the case with coliforms, E. coli, S. aureus, sulphate reducingClostrides
and B. cereus. As for Salmonella spp., only 5 samples have turned out positive: one for S. typhimurium and
one for S. enteritidis (chicken); only one sample from turkey has turned out positive for S. blokley, and two out
of five samples
analysed from quail have been positive for S. typhimurium. About 3% of the samples analyzed have turned
out positive for Listeria monocitogenes, but within the limits of the law. Research on Campylobacter
thermophiles has involved only 50 samples, of which only 5 have resulted positive. These results confirm the
high quality of hygiene and cleanliness of poultry meat, in agreement with that reported in the national
bibliographyand with respect to EU norms.
ID nr. 4159
Tosi° G, Catania S
Laboratory diagnosis of avian mycoplasmosis
Atti della Societa' Italiana di Patologia Aviare 2009 : XLVIII convegno annuale 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 35-40. - 8 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
Pag. 159 di 300 31/08/2011 First author Tosi, Giovanni
In this review the most common methods for the laboratory diagnosis of avian mycoplasmosis are described:
culture methods and agent identification, serological methods (agglutination, ELISA and hemagglutinationinhibition) and molecular methods (in particular the “polymerase chain reaction” or PCR).
ID nr. 4160
Vinco° LJ, Ortali G, Gavazzi L
Respiratory diseases in turkeys, broilers and layers
Atti del XLVIII convegno annuale della Societa' Italiana di Patologia Aviare : 1-2 Aprile 2009, Forli / [s.l. :
s.n., 2009]. - p 17-18
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
First author Vinco, James Leonardo
Respiratory diseases still represent one of the major challanges in poultry production. This in spite of the
severe increase of enteric disorders driven by debatable decisions (ban of growth promoters, bone and meat
meal etc.). In most cases several pathogens are contemporarly involved in respiratory outbreaks thus
complicating diagnosis. This paper reports and describes the main respiratory diseases diagnosed in Italy
between september 2008 and April 2009.
ID nr. 4161
Tamba° M
West Nile Disease: an update
Atti del XLVIII convegno annuale della Societa' Italiana di Patologia Aviare 2009 : 1-2 Aprile 2009, Forli / [s.l.
: s.n., 2009]. - p 19-22. - ref bib 8
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (48 : Forli' : 1-2 Aprile 2009)
First author Tamba, Marco
West Nile virus (WNV) is a mosquito transmitted flavivirus. Birds are involved in the cycle of transmission as
amplifying hosts, but WNV can infect many species of mammals, as well as amphibians and reptiles. Humans
and horses are considered accidental dead-end hosts, but transmission through blood donations, organ
transplants, and the intrauterine route have been reported. In Europe, an enhanced surveillance of WN
infection in humans, horses, birds, and vectors may reveal the presence of the virus in different locations.
However, very little is known of the ecology and natural history of WN virus transmission in Europe and most
WN outbreaks in humans and animals remain unpredictable and difficult to control.
ID nr. 4162
Amadori° M, Razzuoli° E, Farinacci M, Colitti M
Low-dose interferon-alpha treatment can modulate the stress of early weaning in pigs
Annual meeting Epizone : November 19-20, 2009 Maisons - Alfort : Theme 5 / [s.l. : s. n., 2009]. - 1 p.
Meeting Epizone : Maisons - Alfort : November 19-20, 2009)
First author Amadori, Massimo
Interferon-alpha (IFN-alpha) at low concentrations had been previously shown to control in vitro the
expression of inflammatory cytokine genes in swine pulmonary alveolar macrophages. Owing to the above,
we wondered if a similar control action could be exerted by IFN-alpha in piglets during a natural stressing
Pag. 160 di 300 31/08/2011 event like early weaning, characterized by a high proflogistic potential. To this purpose, four Specific
Pathogen Free (SPF) littermate piglets were given human lyrnphoblastoid interferon-alpha for io days in a row
in form of a freeze-dried preparation, mixed in the diet, starting on the day of early weaning at 21 days of age.
The other four littermate piglets served as untreated controls; they received the same daily mass of placebo in
a separate weaning cage. The expression of IFN-alpha, IFN-gamma, IL-1 beta, TNF-alpha and IL-6 genes
was determined in uncultured PBMC by real time PCR. Orally administered IFN-alpha was shown to reduce
alter weaning the expression of the IFN-gamma gene (p<o.o8) and to increase that of the IL-i beta gene
(p<o.o5). There was also a trend to a reduced expression of both IL-6 and TNF-alpha genes. Relatively to the
day of weaning, endogenous IFN-alpha was demonstrated in sera and also PBMC of untreated, control
piglets at day +3, as opposed to day -1. The above modulation of cytokine gene expression and the greater
daily mean weight gain of IFN-treated piglets highlight important regulatory properties of ora] IFN-alpha
treatment at weaning, which probably resemble those of the endogenous cytokine. A greater daily mean
weight gain and significant changes in the time-course of the main inflammatory cytokines in sera were also
shown in a field trial of oral, low-dose IFN-alpha treatment at weaning.
ID nr. 4163
Fabbi° M, Messeri D, Vicari° N, Merlo LM, Pierozzi C, Talini M, Perelli G, Wanderlingh W
An outbreak of tularaemia in Tuscany, Central Italy, linked to a natural spring water
6th International Conference on Tularemia 2009 : Tularemia Network, September 13 - 16, 2009 Berlin,
Germany / [s.l. : s. n., 2009]. - p 108 (
International Conference on Tularemia (6th : Berlin, Germany : September 13-16, 2009)
First author Fabbi, Massimo
Aims: We report an outbreak of tularaemia occurred in the Italian province of Pistoia, Tuscany, linkec to a
natural spring water.
Methods: Between April 2007 and March 2008, 43 cases of tularaemia were confirmed by clinical signs and
serological analysis by tube agglutination test. Most cases (n = 38) occurred between December 2007 and
March 2008. The probable source of infection was natural spring water collected from a small cement basin at
about 950 m of altitude. Water from the spring was usually collected by resident people and tourists, stored in
containers and consumed at home. Water samples from the spring were collected twice, on February 15th
and March 4th, 2008 and tested for Francisella tularensis by PCR, microbiogical methods and mouse
inoculation.
Results: Out of the 43 confirmed cases, 34 (79 %) had been exposed to a common source of water The most
frequently clinical presentation observed was a cervical lymphadenopathy and tonsillitis and sore throat were
observed in a few cases. Antibodies titres of confirmed cases ranged from 1:5C to 1:1,600. Fransisella
tularensis subsp. holarctíca (type B) was demonstrated both times in the natural spring water by PCR and
mice inoculation. Direct culture of the organism from water was unsuccessfull. The outbreak was brought
under control after the demolition of the cement basin and the restoration of the water source as stream.
Conclusions: This large epidemie occurred about 20 years after two last important outbreak of Tularaemia
recorded in northern and centra) Italy (Liguria and Tuscany regions) and confirms the circulation of Fransisella
tularensis in Tuscany. Nevertheless further investigations are needed to explain the route of contamination
and the environmental characteristics that could have maintained or supported the presence or the replication
of Francisella tularensis in the natural spring waters..
ID nr. 4164
Razzuoli° E, Villa° R , Sossi° E, Amadori° M
Evaluation of the interferon-alpha response in pigs after weaning
3rd European Veterinary Immunology Workshop (EVIW) : 10th - 13th September 2009, Berlin, Germany :
Programme & book of abstract / [s.l. : s. n., 2009]. European Veterinary Immunology Workshop (3th : Berlin, Germany : 10th - 13th September 2009)
Project PRC2005002 - Assessment and modulation of the inflammatory effects of early weaning in the piglet
Pag. 161 di 300 31/08/2011 - Amadori Dr. Massimo - Solo - MINSAN - 2005 - 22000,00 EUR - 15/05/2006 0.00.00 14/11/2008 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
First author Razzuoli, Elisabetta
Piglets often show an interferon (IFN)-alpha response in serum after early weaning. This issue was
investigateti in a field trial on sg pigiets weaned at either 22 or 28 days. Ali the pigs remained healthy and
showed the expected weight gains. A low-titered IFN-alpha response was detected in many sera at day +6
alter weaning. The antiviral activity on MDBK cells could be blocked by a monoclonal antibody to porcine IFNalphai. By gel filtration chromatography, the antiviral activity of sera could be traced back to three components
of apparent molecular mass 27/18ho kDa, respectively. A small percentage of peripheral blood mononuclear
cells (PBMC) from severa) pigiets at day +6 were also positive in a flow cytometry assay for intracellular
porcine IFN-alpha. The prevalente of IFN alpha-positive PBMC showed no evident correlation with the serum
IFN-alpha response. The expression of porcine IFN-alpha genes was investigateti by RT-Real Time PCR at
days -1 and +6 in PBMC of 8 piglets. At day -i, the IFN alphas, alpha6 and alphaiz genes were shown to be
expressed in 7 out of 8 pigiets; the IFN alphas, alpha7, alphan, alphaz and alpha4 genes were expressed in
fewer pigs. On the contrary, ali the above genes were not expressed at day +6.Our results are in agreement
with previous reports about constitutive expression and secretion of IFN-alpha. These findings highlight the
importante of type I IFNs as homeostatic agents in the response to environmental stressors.
ID nr. 4165
Catalani E, Amadori° M, Vitali A, Bernabucci U, Nardone A, Lacetera N
Heat shock proteins 72, iimmunological and metabolic parameters in peri-parturient dairy cows
3rd European Veterinary Immunology Workshop (EVIW) : 10th - 13th September 2009, Berlin, Germany :
Programme & book of abstract / [s.l. : s. n., 2009]. - 1 p.
European Veterinary Immunology Workshop (3th : Berlin, Germany : 10th - 13th September 2009)
The present study was aimed at assessing whether the peri-parturient period is associated with changes of
intracellular (IC) or plasma inducible heat shock proteins (Hsp) 72 kQa, and to establish the relationships
between Hsp72, immunological and metabolic parameters in high yielding dairy cows. The study was carried
out in a commercial dairy unit on 3S Hoistein cows. Three, two and one week before the expected calving
date, and one, two, three, four and five weeks alter calving, the body condition score (BCS) of cows was
established, and individual blood samples were taken to measure peripheral blood mononuclear ceil (PBMC)
concentrations of Hsp72, proliferation of PBMC stimulated with Iipopoiysaccharide (LPS), and plasma
concentrations of Hsp72, glucose and nonesterified fatty acids (NEFA). After calving, the IC and plasma
concentrations of Hsp72, and plasma NEFA increased significantly, whereas significantly overvalues were
detected for BCS, plasma glucose and proliferative response of PBMC to IPS. Furthermore, several
significant correlations were found among thee parameters. The time-course of IC and plasma Hsp72 during
the peri-parturient period has not been described before either in dairy cows or in other species. Conversely,
immunological and metabolic changes recorded in this study are in line with previous findings referred to periparturient dairy cows. Further studies are needed to ascertain possible cause and effect relationships
between changes of Hsp72, immune and metabolic parameters, in dairy cows around calving..
ID nr. 4166
Simoni° M, Berneri° R, Pellegrini° E, Ferretti° D, Piro R, Sangiorgi° E
Assessing olive oil : quality using FTNIR spectroscopy
Euroanalysis 2009 : 6 - 10 September 2009 Innsbruck, Austria : Abstracts / [s.l. : s. n., 2009]. Euroanalysis : Innsbruck, Austria : 6 - 10 September 2009)
First author Simoni, Massimo
Quality assessment of final foodstuffs is a fundamental parameter in food production in order to maintain high
Pag. 162 di 300 31/08/2011 quality products. Olive oil, especially extra virgin olive oil, is one of the well recognized important component
of a modern diet, due to its healthy and nutritional quality. Several different analysis are required by official
methods, like COI methods and EC Regulations 2568/1991 methods to distinguish among the different olive
oil categories and to verify oil quality; they appear to be very complicated, time spending and expensive. More
attractive are alternative and non destructive techniques such as spectroscopy. Aim of this work was to verify
the possibility of NIR spectroscopy in assessing oil quality. NIR analysis were performed using a Fourier
transform-NIR spectrometer (NIRFlex N-500, Búchi Laboretechnick AG, Flawill, Switzweriand) in the 4,00010,000 cm-' range at 8 cm-' resolution with NIRCal 5.2 software for calibration. Samples were analysed using
quarti cells (5 mm path length) in transmittance mode. Every spectrum was a 8 scans average. 160 oil
samples coming from different italian regions were analyzed. Good correlations with chemical analysis were
obtained for lipid composition (miristic, palmitic, heptadecanoic, stearic, arachidic, behenic, lignoceric,
palmitoleic, heptadecenoic, oleic, eicosenoic, linoleic and linolenic acids), K 232, K270, AK, peroxides and
acidity; that means the possibility to have a rapid "screening" for virgin olive oil because these analysis are
required, along with organoleptic analysis, to establish the trueness of a virgin olive oil. Further attempts were
made to have good correlations for other, more specific, features, like tocopherols, poliphenols and
antioxidants moieties. According to our results, NIR spectroscopy seems to be a promising technique for oil
quality index.
ID nr. 4167
Simoni° R, Berneri° R, Lorandi° D, Ferretti° D, Piro° R, Sangiorgi° E
Using spectroscopy FTIR analysis in honey characterization and official parameters determination
Euroanalysis 2009 : 6 - 10 September 2009 Innsbruck, Austria / [s.l. : s. n., 2009]. Euroanalysis : Innsbruck, Austria : 6 - 10 September 2009)
First author Simoni, Massimo
Honey is very important in the modern diet due to its nutritional features, its genuineness and healthiness.
Honey composition is quite simple but its analysis is complicated by the different techniques implicated, e.g.
refractometry and conductivity for moisture and ash determinations, liquid chromatography for
hydroxymethylfurfural and sugars determinations, enzymatic reaction for diastase content and titration for
acidity content determination, whereas FTIR spectroscopy allows to have many parameters with a single
determination and only dilution as sample preparation. Aim of this work was to evaluate the effectiveness of
FTIR analysis in honey characterization and official parameters determination. An IR Fourier transformspectrometer for liquid was used (Milkoscan FT2, Foss Electric) with three waves lengths between 240 and
1299 nm (250-405 nm, 445-460 nm e 735-770 nm), with WINISI II 1.50 software for data elaboration. Honey
samples, 2470 at all, were from national competition selections. For parameters calibration sets from 754
(moisture) to 364 samples (minor sugars) were used. Honey samples were diluted with water and analyzed
for the following parameters: moisture, fructose, glucose, sucrose, HMF, diastase, Pfund color, minor sugars
(erlose, maltose, isomaltose, maltulose, melezitose, rhamnose, raffinose, threalose, turanose), proline,
conductivity and polarizer. The principal constituents curves showed good correlations whereas the minor
carbohydrates ones looked promising. With IR technology it was possibie to verify the principal official
requirements and a qualitative valuation of honey quality in a quick and cheap mode. It was even successfully
possibie, for some monolloreal honeys to compare FTIR data with European monofioreal honey profiles
(L.Persano Oddo, Apidologie 35, 2004). Methylantranilate is a marker for citrus honey and promising
correlations were obtained between FTIR and chemical analysis, showing, also in this case, the possibility to
obtain, in a cheap and quick manner, the most part of official parameters and monofloreal features
determination.
ID nr. 4168
Brookes S, Nunez A, Clifford D, Essen S, Irvine R, Van_Reeth K, Untz-Simon G, Loeffen
W,, Foni° E, Larsen L, Matrosovich M, Bublot M, Garcia J, Beer M, Cattoli G, Brown I
Infection dynamics of the current novel swine-like' human influenza A/H1N1 in pigs:
A/California/07/09
Pag. 163 di 300 31/08/2011 8th International Congress of Veterinary Virology : 23rd - 26th August 2009 Budapest - Hungary : 20 years of
ESVV: Integrating Classical and Molecular Virology : Programme & proceeding / [s.l : s.n., 2009]. - p 42 - 3
bib ref
International Congress of Veterinary Virology (8. : Budapest - Hungary : 23rd - 26th August 2009)
ID nr. 4169
Chiapponi° C, Zanni° I, Garbarino° C, Barigazzi° G, Foni° E
Evaluation of the CACO-2 cell line for isolation of swine influenza virus compared to standard
methods
8th International Congress of Veterinary Virology : 23rd - 26th August 2009 Budapest - Hungary : 20 years of
ESVV: Integrating Classical and Molecular Virology : Programme & proceeding / [s.l : s.n., 2009]. - p 186 - 4
bib ref
International Congress of Veterinary Virology (8. : Budapest - Hungary : 23rd - 26th August 2009)
Project PRC2005016 - Epidemiologic surveillance of influenza virus circulation in domestic and wild animals:
detection, antigenic and genomic characterisation of viruses circulating in susceptible species of
domestic animals - Barigazzi Dr. Giuseppe - Solo - MINSAN - 2005 - 77500,00 EUR - 05/06/2006
0.00.00 - 04/06/2008 0.00.00 - 1 - N5 - Parma - Foni Dr.ssa Emanuela - I
2 - N8 - Reggio Emilia - Dottori Dr. Michele - I
3 - N3 - Forlì - Massi Dr.ssa Paola - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
5 - M6 - Mantova - Nigrelli Dr. Arrigo - I
First author Chiapponi, Chiara
During a swine influenza virus (SIV) monitoring programme, 104 samples were submitted to virus isolation
using embryonated chicken eggs (ECE), MDCK cells and CACO-2 cells and 60 SIVs were isolated. The use
of CACO-2 cells was able to isolate 100% of H1N1 and H1N2 subtypes, while the isolation rate for H3N2 was
50%. ECE showed to be able to isolate H1N1 in 44%, H1N2 in 11% and H3N2 in 100% of the cases. MDCK
cells permitted SIV isolation in 56% of H1N1, 3.5% of H1N2 and 38% of H3N2.
ID nr. 4170
Grazioli° S, Pezzoni° G, Cordioli° P, Brocchi° E
Validation of a competitive ELISA for serodiagnosis of PRRS based on recombinant N-protein and
monoclonal antibody
8th International Congress of Veterinary Virology : 23rd - 26th August 2009 Budapest - Hungary : 20 years of
ESVV: Integrating Classical and Molecular Virology : Programme & proceeding / [s.l : s.n., 2009]. - p 193 - 1
bib ref
International Congress of Veterinary Virology (8. : Budapest - Hungary : 23rd - 26th August 2009)
Project PRC2005012 - Production and characterization of recombinant viral proteins as antigens for
serology - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2005 - 68000,00 EUR - 04/09/2006 0.00.00
- 03/09/2009 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Pacciarini Dr.ssa Maria - I
First author Grazioli, Santina
ID nr. 4171
Pezzoni° G , Stercoli° L, Cordioli° P, Brocchi° E
Recombinant NS3 and monoclonal antibodies for serodiagnosis of pestiviruses infection by
competitive ELISA
8th International Congress of Veterinary Virology : 23rd - 26th August 2009 Budapest - Hungary : 20 years of
ESVV: Integrating Classical and Molecular Virology : Programme & proceeding / [s.l : s.n., 2009]. - p 74 - 2
bib ref
International Congress of Veterinary Virology (8. : Budapest - Hungary : 23rd - 26th August 2009)
Pag. 164 di 300 31/08/2011 Project PRC2005012 - Production and characterization of recombinant viral proteins as antigens for
serology - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2005 - 68000,00 EUR - 04/09/2006 0.00.00
- 03/09/2009 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Pacciarini Dr.ssa Maria - I
First author Pezzoni, Giulia
ID nr. 4172
Pezzoni° G, Brocchi° E
A Baculovirus expressed NS3 protein of bovine viral diarrhea virus displays conformational and
antigenical properties as the native protein
8th International Congress of Veterinary Virology : 23rd - 26th August 2009 Budapest - Hungary : 20 years of
ESVV: Integrating Classical and Molecular Virology : Programme & proceeding / [s.l : s.n., 2009]. - p 205 - 1
bib ref
International Congress of Veterinary Virology (8. : Budapest - Hungary : 23rd - 26th August 2009)
Project PRC2005012 - Production and characterization of recombinant viral proteins as antigens for
serology - Pacciarini Dr.ssa Maria - Solo - MINSAN - 2005 - 68000,00 EUR - 04/09/2006 0.00.00
- 03/09/2009 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Pacciarini Dr.ssa Maria - I
First author Pezzoni, Giulia
ID nr. 4173
Sarli G, Ostanello F, Morandi F, Fusaro L, Gnudi M, Bacci B, Nigrelli° A, Alborali° L,
Dottori° M, Vezzoli° F, Barigazzi° G, Fiorentini° L, Sala V, Leotti G, Joisel F
Application of a protocol for the diagnosis of postweaning multisystemic wasting syndrome in Italy
Vet Rec. - Vol. 164 ( 2009). - p 519-523. - 29 bib ref
Impact factor: 1,240
Samples of superficial inguinal and bronchial lymph nodes, ileum, tonsil and lung were taken from three to five
pigs on each of 61 farms with a clinical history of postweaning multisystemic wasting syndrome (PMWS). The
samples were examined histologically and by immunohistochemistry for porcine circovirus type 2 (PCV-2).
PMWS was diagnosed in two stages: first, an evaluation of the haematoxylin and eosin-stained sections that
identified the cases in which the characteristic PCV-2 cytoplasmic inclusion bodies were apparent, and
secondly, a conclusive step in which immunohistochemistry was applied to confirm PMWS in the cases in
which there were positive immunohistochemical results that coincided with lesions indicative of PMWS in at
least one of the lymphoid and/or lung tissues. The location of PCV-2 in specific lesions (cell depletion in
lymphoid organs and interstitial pneumonia) confirmed PMWS in 45 of the 61 farms, 31 of which were also
infected with porcine reproductive and respiratory syndrome virus. The lymphoid tissues were more reliable
than the lungs for the diagnosis of PMWS, both in individual pigs and in groups of pigs, and farm diagnoses
based on a group of pigs were more reliable than diagnoses based on single pigs.
ID nr. 4174
Boselli E, Rodriguez-Estrada MT, Fedrizzi° G, Caboni MF
Cholesterol photosensitised oxidation of beef meat under standard and modified atmosphere at
retail conditions
Meat Sci. - Vol. 81 ( 2009). - p 224-229. - 29 bib ref
Impact factor: 2,183
The effect of the fluorescent light exposure and type of packaging (normal atmosphere and oxygen-rich
atmosphere) was evaluated on the oxidation parameters (peroxides and cholesterol oxidation products) of raw
beef slices placed in packed vessels and refrigerated. The concentration of COPs in meat treated under
modified atmosphere ranged from 0.15 to 0.52 mg/100 g meat (average value of 0.27 mg COPs/100 g meat),
which was twice as much as the average COPs content (0.14 mg/100 g) of meat packed under air (0.04–0.27
Pag. 165 di 300 31/08/2011 mg COPs/100 g meat). The main cholesterol oxide was 7k, which represented about one third of the total
cholesterol oxides, followed by 7ß-OH (20–25% of total COPs), 7a-OH (about 20%) and ß-epoxy (12–18%).
In normal atmosphere, photoxidation was a superficial process, since an inverse correlation between meat
slice weight and COPs content on a lipid basis was observed, unlike in a high oxygen (32%) atmosphere.
ID nr. 4175
Marzadori° F, Frasnelli° M, Raffini° E, Menotta° S, Fedrizzi° G
Evaluation of micro-organisms producing biogenic amines and the concentration of these
metabolites in animal origin food stuf fs which could be correlated to food poisoning episodes
Ing Aliment. - Vol. 6 no 26 ( 2009). - p 54-62 - 25 bib ref
Project PRC2005014 - Evaluation of the presence of biogenic-amines producing microorganisms and of the
concentration of these metabolites in animal-origin food with correlation to possible food-poisoning
episodes - Marzadori Dr. Fausto - Solo - MINSAN - 2005 - 18997,00 EUR - 02/10/2006 0.00.00 31/01/2008 0.00.00 - 1 - N7 - Ravenna (Lugo di Romagna) - Marzadori Dr. Fausto - I
2 - NA - Reparto Merceologia degli Alimenti di origine animale (Bologna) - Fedrizzi Dr. Giorgio - I
First author Marzadori, Fausto
We have shown the results using Moellers broth contoining lysine, orgmine and ornithine inoculoted with
isolcted batteria from fresh and seosoned sausoges, pork muscle, minced chicken aria freshly minced beef.
We then measured the concentrotions of biogenic amines from high pressure liquid chromatogrophs
(HPLC).At the seme time, we counted the histamine forming Enterobacteria (HFB) using the Niven test and
the histamine value from the high pressure liquid chromotogrophs (HPLC). While we believe thot we have con
firmed the validity of using the Moeller broth solution to find micro-orgonism producing biogenic amines, the
data given by the Niven test were discordant and there fore couid not be con firmed. From on overall onolysis
of our data, we believe thet the totol mesophyle microbic titre and the leve] of Enterobacteria can be used as
food sofety indicators to indicate the leve) of risk of biogenic amines in these products os respectively I 10(alla quarta) e 1-10(alla terza) ufc/g.
ID nr. 4176
Canelli° E, Luppi° A, Barbieri° I, Sozzi° E, Lelli° D, Cordioli° P
Phylogenetic characterization of bovine viral diarrohea virus (BVDV) strains isolated in Northern Italy
during the last decade
14th International Symposium for the World Association of Veterinary Laboratory Diagnosticians : 18-20
June Madrid : Abstract / [s.n. : s.l., 2009]. - p 121. - 7 bib ref
International Symposium for the World Association of Veterinary Laboratory Diagnosticians (14 : Madrid :
18-20 June)
First author Canelli, Elena
Bovine viral diarrhoea virus (BVDV) is the causative agent of bovine viral diarrhoea-mucosa) disease
(BVDMD) and fit is responsible for considerable economic losses in cattle industry all over the world. On a
genetic basis, two different genotypes, BVDV-1 and BVDV-2, are distinguished. Genetic typing of BVDVs is
usually based on the genetic diversity of the 5'UTR, NprO and E2 genomic regions. So far, 15 distinct
subgroups within BVDV-1, and 2 within BVDV-2 have been detected [2,3]. Here the results of the genetic
analysis are reported, based on the partial amplification of the 5'UTR and NprO regions, performed to
characterize the BVDV strains isolated in Northern Italy between 1999 and 2008. Genetic relationships of
these strains and eventual temporal) and geographical distributions of the prevailing subtypes were also
assessed. Material & Methods 128 samples collected during the 1999-2008 period from different cattle farms
located in Northern Italy were submitted to the laboratory for routinely virological analysis and tested BVDV
positive. Total RNA was extracted directly from the original samples; retro transcription and amplification
reactions were performed in a one-step PCR system. The primer pairs used were BE–B2 [1;5] and B32-B31
[7] for the 5'UTR and NprO analysis, respectively. The Npr° analysis was carried out on fifty of the strains in
Pag. 166 di 300 31/08/2011 order to confirm the assessment based on the 5'UTR analysis. Following amplification, PCR products of the
expected size were purified and sequenced in an automated sequencer using the sure PCR primers. The
sequences obtained were aligned with those of representative strains present in GenBank and
phylogenetically analyzed. Results Out of the 128 samples analysed 124 were typed as BVDV-1 and 4 as
BVDV-2.On the basis of sequence analysis of the 5'UTR region, eight BVDV-1 subtypes and two BVDV-2
subtypes were detected. Within BVDV-1, the overall branching pattern showed that the Northern Italy strains
principally clustered into subgroups 1 e (36 strains) and 1 b (51 strains). strains of the BVDV-1 b subgroup
formed and ho mogeneous group with an homology value with a median of 97%, while in the BVDV-1 e
cluster, at least two divergent groups (homology lower than 92%)were identified. Other strains grouped as
BVDV-1 a, d, f, h, g and k (6, 9, 7, 6, 2 and 2 strains respectively). The two strains belonging to BVDV-1 k
were compared with strains identified from Switzerland demonstrating an identity of roughly 95%. Following
the 5'UTR region analysis, a small group of 5 strains was genetically lowly correlated to all the other
subgroups within BVDV-1, but the NprO analysis demonstrated that one belonged to 1 g subgroup, and the
others four formed a distinct cluster close to subgroup f. The sequence analysis of the NpfO region of the
remaining strains confirmed the results obtained analysing the 5'UTR region. BVDV-2 strains were a II typed
as belonging to subgroup b, except for one that fell within subgroup a. Overall, from a first analysis, no
interesting correlation was observed regarding the geographical origin of the samples or the year of isolation
and their phylogenetic clustering. Discussion &Conclusione Northern Italy, and particularly the Lombardia and
Emilia Romagna regions, are highly representative of the Italian situation, with about 37% of all national cattle
population heritage. This study confirms the high heterogeneity level of BVDV strains circulating in Italy.
Almost all of the strains (1241128, 96,9%) were assigned to the BVDV-1 genotype (8 subgroups), while only
four (41128, 3,1 %) to BVDV-2 genotype (2 subgroups). BVDV-1 strains mostly belonged to two main
subgroups (1b and 1e}. The high prevalent for BVDV-1 b subgroup is similar to that reported in other
European countries such as the Netherlands, Germany, Belgium, and fortifies the hypothesis that this
subgroup has an European origin. It was showed that group 1 e could be further divided into at least two
clusters, confirming the high variability within this subgroup, already described for Spanish [6] and German
strains [4]. We also detected two strains belonging to the subgroup 1 k, until I now described only in
Switzerland [5], but no evident epidemiological correlation with those strains has been found. A single group
was found to be divergent from all others, and from Npr° analysis fit was considered as forming a separate
cluster within subgroup 1 f. Regarding BVDV 2, the low number of isolates agrees with data from other
Europeans countries, thus confirming that this genotype has a low importance at least in Europe. The high
genetic heterogeneity of BVDVs circulating in Italy is probably linked to the importation of large numbers of
potentially infected cattle from other European countries, in association with the Jack of systematic disease
control measures. Therefore, phylogenetic studies on circulating strains may contribute to a deeper
understanding of the epidemiology and pathogenesis of BVDV infections, and may represent the first step for
the development of efficient diagnosis and control strategies.
ID nr. 4177
Canelli° E, Luppi° A, Lavazza° A, Sandri C, Magnone W, Pascotto E, Gelmetti° D,
Cordioli° P
Description of an encefalomiocarditis virus outbreak in an italian zoo: pathological presentation and
diagnostic course
14th International Symposium for the World Association of Veterinary Laboratory Diagnosticians : 18-20
June Madrid : Abstract / [s.n. : s.l., 2009]. - p 223. - 7 bib ref
International Symposium for the World Association of Veterinary Laboratory Diagnosticians (14 : Madrid :
18-20 June)
First author Canelli, Elena
Encephalomiocarditis virus (EMCV) is a Cardiovirus belonging to the Picornaviridae family and is worldwide
recognized as a pathogen mainly in pigs, but also in non-human primates and in a variety of other wild and
domestic animals. Several fatal outbreaks of EMCV involving different species were described in zoos in
Australia and USA [1:6]. Rodents are probably the natural host and reservoir of the virus, and may spread the
infection to susceptible animals by contaminating feed or water. This work describes the development, lesions
and diagnostic course of an outbreak of EMC affecting primates of an Italian zoo. Material ~ Methods The
Natura Viva zoo in Bussolengo (Verona, Italy) houses various primates, and hosts the most relevant captive
Pag. 167 di 300 31/08/2011 lemur population in Italy. From October 2006 to March 2007, a third of the entire lemur population - a Black
lemur (Eulemur macaco macaco), three ring-tailed lemurs (Lemur Gatta), nine red-ruffed lemurs (Varecia
variegate rubra), two white-fronted lemurs (Eulemur albifrons), two Barbary macaques (Macaca sylvanus) and
two common marmosets (Callithrix jacchus) -lied without showing any apparent clinical sign. The
epidemiological investigation revealed nothing significant, but an increased number of rats inside the zoo and
deratization procedures in progress. Post mortem examination was performed and selected internal organs
(including lung, heart, small and gross intestine, kidneys, liver, brain and spleen) were sampled for diagnostic
investigation. For histopathology, portion of the different organs were fixed in 10% buffered formalin,
embedded in paraffin and 5 pm-thick sections were stained with haematoxylin-eosin. Immunohistochemistry
was performed using a biotin-streptavidin method employing the 3E5 EMCV monoclonal antibody (mAb)
produced by IZSLER. Parasitologicalanti bacteriological exams were performed using standard methods.
Toxicological examination was focused to detect rodenticidals. For virological analysis, the different organs
were homogenised in MEM-A, clarified and inoculated on VERO and BHK21 cells. Some rats were also
captured and sampled for virological examination. Results In all cases the clinical presentation was sudden
death without any evident symptoms or external lesions. At necropsy, the main lesions were in the cardiorespiratory system: cardiomegaly and grey-white necrotic foci of the myocardium, accumulation of excessive
fluid in the body cavities (hydrothorax and ascite ), hydropericardium and severe pulmonary oedema.
Histologically, hydropic degeneration with focal areas of necrosis and different degrees of lymphocytes and
neutrofilic granulocytes interstitial infiltrations were observed in the myocardium. Degenerated myocardial
fibres were hypertrophic, with eosinophilic and amorphous cytoplasm and elongated nuclei showing with
clumped chromatin or pyknosis. Examination of the small and gross intestine, kidneys, liver and spleen ditty
not show any specific changes. Using immunohistochemistry, EMCV immunopositive myocardiocites were
observed in all cases; distribution and intensity of the staining were in accordance with the severity of the
histological lesions. All the other organs tested negative. No significant bacteria or parasite were identified
from any of the animals and toxicological investigations were all negative; but viral cytopathic effect was
detected at 24-72h post-inoculation of pathological tissues on both VERO and BHK21 cells. The isolated virus
was identified as EMCV by using both a mAb-based sandwich ELISA and immunoelectronmicroscopy. All
sampled rats resulted negative at viral isolation. Discussion & Conclusions This report describes an outbreak
of ECMV occurring in zoo's captive primates in Italy and confirms the risk that this virus poses for such
animals and their high susceptibility to the infection [1;2;4;5;7]. Thus, in our opinion EMCV should always be
included in differential diagnosis when sudden death of primates without any evident symptoms occurs, in
particular when myocarditis with myocardial degeneration is reported. Even if ECMV was not isolated from
captured rats, rodents should be considered an important risk factor for EMCV infection and their potential
role in spreading the virus calls for a regular and adequate application of rodent control programs in zoos. In
this case, a rodent control plan was strengthened involving all food storage sites and food preparation areas.
Unfortunately, to avoid dangerous stress to the animals it was not possible to verify their serological status.
Further investigations will be necessary to asses if, as it has been previously suggested [6], immunity may not
be protective against later exposure to this virus. Finally, considering the zoonotic nature of ECMV, these
finding are nevertheless of public concern.
ID nr. 4178
Lelli° D, Moreno_Martin° A, Lavazza° A, Canelli° E, Sozzi° E, Brocchi° E, Cordioli° P
Neuraminidase avian influenza viruses identification using monoclonal antibodies
14th International Symposium for the World Association of Veterinary Laboratory Diagnosticians : 18-20
June Madrid : Abstract / [s.n. : s.l., 2009]. - p 98. - 2 bib ref
International Symposium for the World Association of Veterinary Laboratory Diagnosticians (14 : Madrid :
18-20 June)
First author Lelli, Davide
ID nr. 4179
More SJ, Cameron AR, Greiner M, Clifton-Hadleyd RS, Correia_Rodeia S, Bakker D,
Salman MD; Sharph JM, De_Massis F, Aranaz A, Boniotti° MB, Gaffuri° A, Have P,
Verlooe D, Woodfordl M, Wierupm M
Defining output-based standards to achieve and maintain tuberculosis freedom in farmed deer, with
Pag. 168 di 300 31/08/2011 reference to member states of the European Union
Prev Vet Med. - Vol. 90 ( 2009). - p 254-267. - 33 bib ref
Impact factor: 1,506
Within the European Union (EU), detailed legislation has been developed for cattle, but not deer, to minimise
disease risks associated with trade in animals and animal products. This legislation is expressed as inputbased standards, providing a detailed outline of the activity required (for example, testing of animals and
application of defined control measures), on the expectation that an adequate output (for example, confidence
in freedom) will be achieved. Input-based standards are at odds with the increasing shift towards outputbased standards, particularly in OIE rules governing international trade. In this paper, we define output-based
standards to achieve and maintain freedom from tuberculosis (TB) in farmed deer, with reference to EU
member states. After considering the probability of freedom achieved for cattle under existing EU legislation,
we defined a ‘free farmed deer holding’ as one with a probability of freedom from infection of at least 99%. We
then developed an epidemiological model of TB surveillance systems for deer holdings, incorporating different
surveillance strategies, including combinations of diagnostic tests, and a variety of different scenarios relating
to the potential for introduction of infection. A range of surveillance strategies were identified to achieve and
maintain a free farmed deer holding, and worked examples are presented. The surveillance system sensitivity
for varying combinations of screening and confirmatory tests in live animals, animals at slaughter and on-farm
deaths is also presented. Using a single test at a single point in time, none of the TB tests routinely used in
farmed deer is able to achieve an acceptable probability of TB freedom. If repeat testing were undertaken, an
acceptable probability of TB freedom could be achieved, with differing combinations of the surveillance
system sensitivity, frequency of testing and risk of introduction. The probability of introduction of infection
through the importation of infected deer was influenced by the use of a pre-movement test (assumed 90% test
sensitivity and negative test results), the TB prevalence in the source herd and the number of animals
imported. A surveillance system sensitivity of at least 81% was achieved with different combinations of annual
live animal surveillance and surveillance of animals at slaughter or on-farm deaths. This methodology has
broad applicability and could also be extended to other diseases in both deer and other species with
relevance to trade in animals and animal products.
ID nr. 4180
Fabbi M°
Tularemia: pathology, zoonotic aspects and bioterrorism
XI Congresso Nazionale SIDiLV : Parma, Centro Congressi, Comune di Parma 30 Settembre - 2 Ottobre
2009 : volume degli atti / [s.l. : Societa' Italiana Diagnostica di Laboratorio Veterinaria ( SIDiLV ), 2009]. - p
50-51
Congresso Nazionale Societa' Italiana Diagnostica di Laboratorio Veterinaria (SIDiLV) (11. : Parma : 30
Settembre - 2 Ottobre 2009)
First author Fabbi, Massimo
Tularemia is a zoonotic disease recognized in more than 70 species of vertebrates, albeit with varying
degrees of susceptibility. Mainly affects rodents and lagomorphs (hares, rabbits, beaver, prairie dog, hamster,
lemming, squirrel, voles, mice, rats, etc.), with broad spectrum of host (foxes, ferrets, weasels, hedgehogs,
cows, sheep, pig, dog, cat, birds). In nature it is usually disease of rodents and lagomorphs in which induce
fata] septicemia . The causative agent is Francisella tularensis, a small gram-negative bacteria easily
transmissible to humans through various routes with the development of different clinical pictures in different
severity. To its characteristics of extreme spread and infectivity is present in the A List of microorganisms
employed in bioterrorism. Known for at least a century, the disease is present in North America, Asia and
Centrai and Eastern Europe. in Italy there is sporadic or limited in time and space (Emilia-Romagna,
Lombardy, Piedmont, Tuscany, Liguria, Umbria). The humans epidemic events in Italy (mainly Tuscany and
Liguria) has been linked to contaminated water coming from nonchlorinated and non controlled water
systems. Sporadic episodes (Lombardy and Piedmont) are linked to contact with hares or rodents (handling,
skinning) or from bites of arthropod vectors (ticks). Attention should be paid to the risk of introduction of the
disease through the importation of hares from eastern Europe for restocking because of the endemic situation
that persists in these countries in respect of tularemia.
Pag. 169 di 300 31/08/2011 ID nr. 4181
Natale A, Patregnani T, Tagliabue° S, Alborali° L, Ferronato A, Bucci G, Toffan C, Ceglie
L, Catania S, Bonfanti L
Management of a leptospirosis outbreak due to Leptospira interrogans serovar Hardjo in a dairy farm
in the Po Valley, Italy
Large Anim Rev. - Vol. 15 no 4 ( 2009). - p 153-157. - 11 bib ref
During October 2007 a dairy cow serum collected after abortion tested positive to L. interrogans serovar
Hardjo (serogroup Sejroe) with a titre of 1:400. After serological confirmation an official outbreak was declared
and restrictions measures were put in place (D.P.R. 320/54, O.M. 4 settembre 1985). The active control of the
outbreak included direct prophylaxis measures, serological monitoring, molecular and microbiological
diagnosis on urine samples collected from serologically positive animals. Antibiotic therapy was administered
to positive animals. Based on this acquired experience, our study suggests an alternative approach for
reducing the period of sanitary and restriction measures.
ID nr. 4182
Bardasi° L, Bonilauri° P, Rugna° G, Galletti° G, Fedrizzi° G, Santandrea G, Gandolfi P,
Vecchi G, Merialdi° G
Growth of naturally occuring Listeria innocua in coppa di testa
XIX Convegno Nazionale AIVI : la filiera corta: lo stato dell’arte, la tutela del consumatore, le difficoltà e le
prospettive : Perugia 24 - 25 - 26 Giugno 2009 / [s.n. : s.l., 2009]. - p 47
Convegno Nazionale AIVI (19 : Perugia : 24 - 25 - 26 Giugno 2009)
Project PRC2007001 - Determination of quality and safety features based on predictive microbiology and
risk assessment of traditional regional food products - Finazzi Dr. Guido CON DELIBERA DG N. 461
DEL 17/09/2010 SOSTITUITO DR. BONI CON DR. FINAZZI - Solo - MINSAN - 2007 - 615000,00
EUR - 01/09/2008 0.00.00 - 31/08/2010 0.00.00 - 1 - I3 - Reparto Microbiologia e Parassitologia
degli Alimenti e Sorveglianza Epidemiologica - Boni Dr. Paolo - I
2 - M6 - Sezione diagnostica provinciale di Mantova - Franzini Giuliana - I
3 - M9 - Sezione diagnostica provinciale di Sondrio - Bertoletti Dr.ssa Irene - I
4 - N1 - Sezione diagnostica provinciale di Bologna - Bardasi Dr.ssa Lia - I
5 - N6 - Sezione diagnostica provinciale di Piacenza - Cammi Dr.ssa Giuliana - I
6 - N7 - Sezione diagnostica provinciale di Ravenna (Lugo di Romagna) - Raffini Dr. ssa
Elisabetta - I
First author Bardasi, Lia
Coppa di testa is a traditional cookcd pork salami produced in different ltalian regions. The main raw material
is deboned meat of pork head with the addition of tongue and rind. After a long (3-5 h) high temperature
(97°C) cooking, additives and flavourings are added and the salami is prepared. After cooling the salami is
often portioned and vacuum-packaged. In this study the growth of naturally occurring contaminati on of
Listcria innocua in thrce batches of vacuum packaged Coppa di testa, stored at 4°C for 80 days, is described.
The average µmax was 0.24 (days-1) and thc average doubling time was 2.87 days. The maximum growth
level ranged from 4.90 to 8.17 (log10 cfu/g). Thesc results indicate that Coppa di testa definitely supports the
growth of Listeria innocua in the considered storage conditions. Taking into account that at 4°C Listcria
monocytogenes strains are associated with higher grow rates than L. innocua, these results emphasize the
importance of preventing Listcria monocytogenes contamination in the produetion stages following cooking.
ID nr. 4184
Ferrari° M, Borghetti P, Foni° E, Robotti° C, Di_Lecce R, Corradi A, Petrini° S, Bottarelli E
Pag. 170 di 300 31/08/2011 Pathogenesis and subsequent cross-protection of influenza virus infection in pigs sustained by an
H1N2 strain
Zoonoses Public Health. - Vol. 57 ( 2010). - p 273-280. - 28 bib ref
Impact factor: 1,912
First author Ferrari, Maura
The H1N1, H3N2 and, more recently, H1N2 subtypes of influenza A virus are presently co-circulating in swine
herds in several countries. The objectives of this study were to investigate the pathogenesis of
Sw/Italy/1521/98 (H1N2) influenza virus, isolated from respiratory tissues of pigs from herds in Northern Italy,
and to evaluate its potential cross-protection against the Sw/Fin/2899/82 (H1N1) strain. In the pathogenesis
test, eight pigs were intranasally infected with H1N2 virus; at pre-determined intervals, these animals were
killed and necropsied, along with eight uninfected animals. In the cross-protection test, sixteen pigs were
infected by intranasal (i.n.) and intratracheal (i.t.) routes with either H1N2 or H1N1 virus. Twenty days later, all
pigs were challenged (by the same route), with either the homologous H1N2 or heterologous H1N1 virus
strains. Control group was inoculated with culture medium alone. On post-challenge days (PCD) 1 and 3, two
pigs from each infected group, along with one control pig, were killed. Clinical, virological, serological and
histopathological investigations were performed in both the pathogenicity and cross-protection tests. In the
pathogenicity test, mild clinical signs were observed in two pigs during 3 and 4 days, respectively. Virus was
isolated from two pigs over 6 days and from lung samples of pigs killed on post-infection days 2 and 4.
Seroconversion was detected in the two infected animals killed 15 days after infection. In the cross-protection
study, mild clinical respiratory signs were detected in all pigs infected with either the H1N2 or H1N1 virus. The
virus was isolated from nasal swabs of almost all pigs till 6 days. After the challenge infection, the pigs
remained clinically healthy and virus isolation from the nasal secretions or lung samples was sporadic.
Antibody titres in H1N1 or H1N2 infected groups were similar, whereas the H1N2 sub-type induced less
protection against re-infection by homologous and heterologous virus than H1N1 sub-type. The controls had
no signs of the disease. In the H1N2 infected pigs, a reduced number of goblet cells in nasal and tracheal
mucosa and small foci of lymphomononuclear cell infiltrates in the submucosa were detected. Furthermore,
the goblet cell reduction was related to the time of infection. Diffuse mild interstitial pneumonia was also
recorded in pigs infected with the H1N2 virus and challenged with either H1N1or H1N2 pigs. These studies
showed the moderate virulence of the H1N2 virus and a partial cross-protection against heterologous
infection.
ID nr. 4185
Decaro N, Mari V, Campolo M, Lorusso A, Camero M, Elia G, Martella V, Cordioli° P,
Enjuanes L, Buonavoglia C
Recombinant canine coronaviruses related to transmissible gastroenteritis virus of swine are
circulating in dogs
J Virol. - Vol. 83 no 3 ( 2009). - p 1532-1537. - 28 bib ref
Impact factor: 5,308
Project PRF2007202 - Mammal coronaviruses: molecular epidemiology, vaccine development And
implications for animal and human health - Cordioli Dr. Paolo - Capofila - MINSAN - 2007 300000,00 EUR - 01/09/2009 0.00.00 - 31/08/2011 0.00.00 - 1 - U1 - REPARTO VIROLOGIA Cordioli Dr. Paolo - I
2 - Università di Bari - Dipartimento di Sanità Pubblica e Zootecnica - Decaro Prof. Nicola - E
3 - IstitutoZooprofilattico della Sardegna - Oggiano Dr.ssa Annalisa - E
4 - Istituto Zooprofilattico della Sicilia - Guercio Dr.ssa Annalisa - E
5 - Istituo Zooprofilattico delle Venezie - Nardelli Dr. Stefano - E
Four canine coronavirus type II (CCoV-II) strains were identified in the guts and internal organs of pups which
had died of acute gastroenteritis. The CCoV-II strains were strictly related to porcine transmissible
gastroenteritis virus (TGEV) in the N-terminal domain of the spike protein, whereas in the other parts of the
genome, a higher genetic relatedness to recent CCoV-II isolates was observed. Experimental infection of
dogs with a TGEV-like isolate induced mild gastroenteritis without any systemic involvement. By virus
neutralization tests, antigenic differences between reference and TGEV-like CCoVs were found. Our data
Pag. 171 di 300 31/08/2011 support the potential recombinant origin of the TGEV-like CCoVs.
ID nr. 4186
Losi° CG, Sossi° E, Ferrari° S, Villa° R, Ferrari° M
An innovative method for cell line identification and interspecies crosscontraminations: cytochrome
B polymerase chain reaction - restriction fragment length polymorphism analysis
42° International Symposium of animal production "New analytical technologies : tools and implementation
strategies in animal science" / [s.l. : s.n., 2009]. - p 121-130. - bib ref 9
International Symposium of animal production (42
First author Losi Claretta_Gioia
One of the major problems in cell culture technology is the misidentification or crosscontaminations of cell
lines. According to that, the authentication of all cell lines collected in the Cell Culture Center in Brescia is
routinely performed with the aim to confirm the species of origin of each substrate. Currently, this investigation
is performed by isoenzyme analysis: however, this method displays several disadvantages such as it is time
consuming, it produces variable results and it is difficult to standardize. The need to improve isoenzyme
analysis led to the development of a novel technique based on the application of a Polymerase Chain
Reaction-Restriction Fragment Length Polymorphism analysis to a portion of cytochrome b. This method
allows to identify 30 different animal species and to detect interspecies cross contaminations with a sensitivity
at least similar to that of isoenzyme analysis.
ID nr. 4187
Cordioli° P
Diagnostic methods in avian viral infections
Atti della Societa' Italiana di Patologia Aviare 2008 : XLVII convegno annuale 2 Ottobre 2008, Forli / [s.l. :
s.n., 2008]. - p 80-83
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (47 : Forli' : 2 Ottobre)
First author Cordioli, Paolo
The common methods used in avian pathology for the diagnostic of viral infections are reviewed.
ID nr. 4189
Tosi° G
Group 1 adenovirus infections
Atti della Societa' Italiana di Patologia Aviare 2008 : XLVII convegno annuale 2 Ottobre 2008, Forli / [s.l. :
s.n., 2008]. - p 11-18
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (47 : Forli' : 2 Ottobre)
First author Tosi, Giovanni
In this review the most common diseases caused by group 1 fowl adenovirus are described: quail bronchitis,
pancreatitis in guinea fowl, inclusion body hepatitis, hydropericardium syndrome and respiratory diseases
caused by CELO virus. In addition, laboratory diagnosis and intervention strategies of these diseases are
reported.
Pag. 172 di 300 31/08/2011 ID nr. 4190
Sozzi° E, Luppi° A, Lelli° D, Moreno_Martin° A, Canelli° E, Brocchi° E, Lavazza° A,
Cordioli° P
Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine
epidemic diarrhoea virus
Res Vet Sci. - Vol. 88 no 1 ( 2010). - p166-168. - 17 bib ref
Impact factor: 1,345
Project PRC2003024 - Set-up of reagents and diagnostic methods for the study of animal coronaviruses Cordioli Dr. Paolo - Capofila - MINSAN - 2003 - 76500,00 EUR - 26/04/2004 0.00.00 - 25/10/2006
0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
2 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
3 - IZSVE - Nardelli Dr. Stefano - E
First author Sozzi, Enrica
Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. A double
antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal
antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The DAS-ELISA was
compared with RT-PCR in the examination of 506 specimens collected during 2006–2007 from pigs
originating from different farms located in the Po valley. Both faecal samples obtained directly from the rectum
of live animals showing clinical signs and intestinal samples collected from the caecum of deceased pigs were
included in the study. The correlation between the two methods was higher when testing faecal samples (K =
0.97, 95% CI: 0.94–1.00) than testing intestinal samples (K = 0.62, 95% CI: 0.35–0.89). The use of ELISA
technology provided an efficient and effective mean of evaluating the presence of coronavirus PED antigen in
field samples and indicates that this procedure is a very useful tool in epidemiological studies..
ID nr. 4191
Vitali A, Segnalini M, Bertocchi° L, Bernabucci U, Nardone A, Lacetera N
Seasonal pattern of mortality and relationships between mortality and temperature-humidity index in
dairy cows
J Dairy Sci. - Vol. v 92 no 8 ( 2009). - p 3781-3790. - 24 bib ref
Impact factor: 2,486
The 2 studies described investigated seasonal variations of mortality and temperature-humidity index (THI)–
mortality relationships in dairy cows. Mortality data were extracted from the Italian Bovine Spongiform
Encephalopathy databases, which contain records on cows older than 24 mo that died on a farm from all
causes (98% of total records), were slaughtered in an emergency state, or were sent for normal slaughter but
were sick in the preslaughter inspection (2% of total records). Both studies evaluated mortality data during a
6-yr period (2002 to 2007). The seasonal pattern study was conducted throughout Italy and was based on
320,120 deaths. An association between season and deaths was found for all 6 yr. Summer and spring were
the seasons with the highest and lowest frequency of deaths (15,773.3 ± 2,861 and 11,619.3 ± 792.3),
respectively, and within summer months, the number of deaths in July and August (5,435 ± 284 and 5,756 ±
676.2, respectively) was higher than in June (4,839 ± 344.8). The THI–mortality relationships study was
carried out only for deaths (51,240) reported for the Lombardia and Emilia Romagna regions. For this study,
the mortality databases were integrated with THI data, which were calculated by using data from 73 weather
stations. Each farm where deaths were recorded was assigned the THI values (maximum and minimum)
calculated at the closest weather station for each day the events (deaths) were reported. Analysis of data
indicated that approximate THI values of 80 and 70 were the maximum and minimum THI, respectively, above
which the number of deaths in dairy farms starts to increase. Maximum and minimum THI values of 87 and 77
were the upper critical THI above which the risk of death for dairy cows becomes maximum. This study
defined quantitative relationships between mortality risk and THI in dairy cows and may help to provide
emergency interventions and mitigation measures, which may ensure survival of dairy cows and reduce
replacement costs associated with heat stress-related mortality.
Pag. 173 di 300 31/08/2011 ID nr. 4193
Badan M, Dorigo M, Dalvit P, Zambon D, Biancotto G, Bertocchi° L, Varisco° G, Barberio
A
Factors affecting Aflatoxin M1 excretion into milk
Buiatria. - Vol. 4 no 3 ( 2009). - p 39-44. - 5 bib ref
Project PRC2003026 - Study and demonstration of feed-management techniques for the reduction of
mycotoxins carry-over in milk and harmonisation of analytical methods through production of
reference material - Varisco Dr. Giorgio - Capofila - MINSAN - 2003 - 60000,00 EUR - 26/04/2004
0.00.00 - 25/04/2006 0.00.00 - 1 - I4 - Reparto produzioni zootecniche e sorveglianza
epidemiologica degli allevamenti - Bolzoni Dr. Giuseppe - I
2 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti Bertocchi Dr. Luigi - I
3 - IZSVE - Barberio Dr. Antonio - E
4 - IZSLT - Rosati Dr. Remo - E
5 - IZSUM - Valiani Dr. Andrea - E
ID nr. 4194
Dorigo M, Badan M, Dalvit P, Zambon D, Biancotto G, Bertocchi° L, Varisco° G, Barberio
A
Carry-over in milk of low doses of aflatoxin B1
Buiatria. - Vol. 4 no 3 ( 2009). - p 33-38. - 5 bib ref
Project PRC2003026 - Study and demonstration of feed-management techniques for the reduction of
mycotoxins carry-over in milk and harmonisation of analytical methods through production of
reference material - Varisco Dr. Giorgio - Capofila - MINSAN - 2003 - 60000,00 EUR - 26/04/2004
0.00.00 - 25/04/2006 0.00.00 - 1 - I4 - Reparto produzioni zootecniche e sorveglianza
epidemiologica degli allevamenti - Bolzoni Dr. Giuseppe - I
2 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti Bertocchi Dr. Luigi - I
3 - IZSVE - Barberio Dr. Antonio - E
4 - IZSLT - Rosati Dr. Remo - E
5 - IZSUM - Valiani Dr. Andrea - E
ID nr. 4195
Bertocchi° L, Cerioli° M
Biosecurity in Veterinary : biosecurity in bovine breeding
La biosicurezza in veterinaria / edito a cura della Fondazione Iniziative Zooprofilattiche e Zootecniche. Brescia : Fondazione Iniziative Zooprofilattiche e Zootecniche, 2009. - (Atti delle giornate di Studio
Fondazione Iniziative Zooprofilattiche e Zootecniche ; 74) p 21-46. - 37 bib ref
This work examines the means and management practice procedures to prevent and limit the introduction
and spread of biological risks and then the development of diseases and pathological states in cow. This
work is divided into two parts: the first one general that speaks about milk herd management and the second
one special that treats about the most important farm pathologies.
ID nr. 4196
Pignatelli J, Jimenez M, Luque MT, Rejas MT, Lavazza° A, Rodriguez D
Molecular characterization of a new PToV strain : evolutionary implications
Virus Res. - Vol. 143 ( 2009). - p 33-43. - 56 bib ref
Pag. 174 di 300 31/08/2011 Impact factor: 2,429
Toroviruses are emergent viruses, belonging to the Nidovirales order, that remain mostly ignored, despite they
are able to infect different species of domestic animals and humans, causing enteric diseases and diarrhea.
Thus far, only five variants of porcine torovirus (PToV) have been identified. In this report we describe the
identification and partial characterization of a new strain of porcine torovirus (PToV-BRES) that was detected
by RT-PCR in a swine faecal specimen from a farm in Brescia (Italy). The complete genes coding for the
nucleocapsid (N), hemagglutinin-esterase (HE) and membrane (M) proteins were amplified, and sequence
analysis showed that PToV-BRES is a new PToV strain that, based on the HE gene sequence, is
phylogenetically related to P4 strain, that was up to now the only member of a distinct PToV lineage. The
nucleocapsid protein from PToV-BRES was expressed in insect cells as a his-tagged protein, purified by
affinity chromatography and used to develop an ELISA method to detect antibodies against PToV. This assay
was evaluated using a serum collection including 45 samples from three commercial farms from Spain. High
antibody prevalence against PToV was observed in the three farms, both in adult animals and in piglets,
which could suggest that PToV might be endemic in Spanish porcine population. The ELISA method
developed in this work could be useful in future epidemiological surveys about toroviruses.
ID nr. 4200
Lavazza° A, Cerioli° M, Grilli° G
Biosecurity in veterinary medicine: biosecurity in bovine breeding
La biosicurezza in veterinaria / edito a cura della Fondazione Iniziative Zooprofilattiche e Zootecniche. Brescia : Fondazione Iniziative Zooprofilattiche e Zootecniche, 2009. - (Atti delle giornate di Studio
Fondazione Iniziative Zooprofilattiche e Zootecniche ; 74) p 91-120. - 30 bib ref
This work examines the means and management practice procedures to prevent and limit the introduction
and spread of biological risks and then the development of diseases and pathological states in cow. This
work is divided into two parts: the first one general that speaks about milk herd management and the second
one special that treats about the most important farm pathologies.
ID nr. 4201
Martelli P, Lavazza° A, Nigrelli° AD, Merialdi° G, Alborali° G, Pensaert MB
Epidemic of diarrhoea caused by porcine epidemic diarrhoea virus in Italy
Vet Rec. - Vol. 162 ( 2008). - p 307-310. - 17 bib ref
Impact factor: 1,087
There was an epidemic of diarrhoea affecting pigs of all ages in Italy between May 2005 and June 2006. In 63
herds the cause was confirmed as porcine epidemic diarrhoea virus by electron microscopy, immunoelectron
microscopy, PCR and serology. Watery diarrhoea without mucus and blood was usually associated with a
reduction of feed consumption. In farrowing-to-weaning herds, diarrhoea affected the sows and suckling
piglets, and the mortality in newborn piglets was up to 34 per cent. In growers and fatteners the morbidity
ranged from 20 to 80 per cent, but there was either no mortality or it was very low. Depending on the size of
the herd and the type of operation, the clinical disease lasted for weeks or months.
ID nr. 4202
De_Bernardinis° F, Finazzi° G, Daminelli° P, Bertolassi° R, Boni° P, Bonometti° E, Boni°
P
Dynamic of several pathogens in artificially contaminated Salame Piacentino DOP
Ind Aliment (Pinerolo). - Vol. 48 n 497 ( 2009). - p 40-45. - 6 bib ref
Pag. 175 di 300 31/08/2011 Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author De_Bernardis, Federica
ID nr. 4204
Renzi° M
Rabbits : gastrointestinal bacterial diseases
Obiet Docum Vet. - Vol. 30 no 1 ( 2009). - p 13-21. - 59 bib ref
First author Renzi, Maria
The aim of this work is to describe the gastrointestinal bacterial disease as a major cause of economic losses
in intesive rabbit production. The aetiological agents ( Escherichia coli, Clostridium sp. Salmonella sp.and
Klebsiella pneumoniae ) and the role of the gut barrier, caecotropy and caecal metabolism in digestive
disorders are reviewed.
ID nr. 4205
Laroucau K, Vorimore F, Bertin C, Mohamad KY, Thierry S, Hermann W, Maingourd
C,Pourcel C, Longbottom D, Magnino° S, Sachse K, Vretou E, Rodolakis A
Genotyping of Chlamydophila abortus strains by multilocus VNTR analysis
Vet Microbiol. - Vol. 137 ( 2009). - p 335-344. - 22 bib ref
Impact factor: 2,370
Chlamydophila (C.) abortus is the causative agent of ovine enzootic abortion with zoonotic potential whose
epidemiology has been held back because of the obligate intracellular habitat of the bacterium. In the present
study, we report on a molecular typing method termed multiple loci variable number of tandem repeats
(VNTR) Analysis (MLVA) for exploring the diversity of C. abortus. An initial analysis performed with 34
selected genetic loci on 34 ruminant strains including the variant Greek strains LLG and POS resulted in the
identification of five polymorphic loci, confirming the widely held notion that C. abortus is a very homogeneous
species. Analysis of additional 111 samples with the selected five loci resulted in the classification of all
strains into six genotypes with distinct molecular patterns termed genotypes [1] through [6]. Interestingly, the
classification of the isolates in the six genotypes was partly related to their geographical origin. Direct
examination of clinical samples proved the MLVA to be suitable for direct typing. Analysis of the genomic
sequences in six C. abortus prototypes of amplicons generated with each of the five selected VNTR primers
revealed that variation between genotypes was caused by the presence or absence of coding tandem repeats
in three loci. Amplification of Chlamydophila psittaci reference strains with the five selected VNTR primers and
of the six C. abortus prototype strains with the eight VNTR primers established for the typing of C. psittaci
[Laroucau, K., Thierry, S., Vorimore, F., Blanco, K., Kaleta, E., Hoop, R., Magnino, S., Vanrompay, D.,
Sachse, K., Myers, G.S., Bavoil, P.M., Vergnaud, G., Pourcel, C., 2008. High resolution typing of
Chlamydophila psittaci by multilocus VNTR analysis (MLVA). Infect. Genet. Evol. 8(2), 171–181] showed that
both MLVA typing systems were species-specific when all respective VNTR primer sets were used. In
conclusion, the newly developed MLVA system provides a highly sensitive, high-resolution and easy-toperform tool for the differentiation of C. abortus isolates of different origin, which is suitable for molecular
epidemiological studies.
Pag. 176 di 300 31/08/2011 ID nr. 4206
Sachse K, Laroucau K, Vorimore F, Magnino° S, Feige J, Müller W, Kube S, Hotzel H,
Schubert E, Slickers P, Ehricht R
DNA microarray-based genotyping of Chlamydophila psittaci strains from culture and clinical
samples
Vet Microbiol. - Vol. 135 ( 2009). - p 22-30. - 23 bib ref
Impact factor: 2,370
The avian and human pathogen Chlamydophila (C.) psittaci represents a genetically heterogeneous species.
To facilitate epidemiological surveys, more rapid yet highly specific molecular tests are needed. Currently
used typing methods, i.e. serotyping and PCR-RFLP, have only limited sensitivity and are incapable of
covering the wide spectrum of naturally occurring types of C. psittaci strains. In the present study, a new DNA
microarray assay based on the ArrayTube® (AT) technology was used to genotype C. psittaci in 98 isolates
and 23 clinical tissue samples. The present array carries 35 oligonucleotide probes derived from variable
domains 2 and 4 of the ompA gene. The assay proved highly sensitive, allowing correct genotyping of DNA
from 2 inclusion-forming units. The results of DNA microarray genotyping of cultured strains proved highly
concordant with the data from PCR-RFLP typing and serotyping. Sequencing of the ompA gene served as the
reference test to verify the accuracy of AT genotyping results. In 15 instances (15.3%), strains were
successfully typed by the AT assay, while serotyping and/or PCR-RFLP genotyping failed to produce
unambiguous results. Eleven of these samples were ompA sequenced to confirm the AT findings. In addition
to the currently accepted nine ompA genotypes, the microarray test was shown to recognise new provisional
genotypes, such as Mat116 and YP84. In conclusion, the new AT assay proved to be suitable for rapid,
sensitive and reproducible genotyping of C. psittaci strains and can be recommended for routine diagnosis.
ID nr. 4207
Donati M, Di_Francesco A, Baldelli R, Magnino° S, Pignanelli SI, Shurdhi A, Delucca F,
Cevenini R
In vitro detection of neutralizing antibodies to Chlamydia suis in pig sera
Vet Rec. - Vol. 164 ( 2009). - p 173-174. - 13 bib ref
Impact factor: 1,240
ID nr. 4208
Ferreri AJN, Dolcetti R, Magnino° S, Doglioni C, Ponzoni M
Chlamydial infection : the link with ocular adnexal lymphomas
Nat Rev Clin Oncol. - Vol. 6 ( 2009). - p 658-669. - 115 bib ref
Impact factor: 9,113
Chlamydiae are obligate intracellular bacteria that grow in eukaryotic cells and cause a wide spectrum of
diseases. They can establish persistent infections, are mitogenic in vitro, promote polyclonal cell proliferation
in vivo and induce resistance to apoptosis in infected cells—properties that might contribute to tumorigenesis.
In fact, Chlamydophila psittaci (Cp) has been linked to the development and maintenance of ocular adnexal
marginal zone B-cell lymphoma (OAMZL). In this indolent malignancy, Cp is transported by monocytes and
macrophages and causes both local and systemic infection. Cp elementary bodies are viable and infectious in
the conjunctiva and peripheral blood of patients with OAMZL. Bacterial eradication with antibiotic therapy is
often followed by lymphoma regression. Despite recent advances in the understanding of this bacterium–
lymphoma association, several questions remain unanswered. For instance, prevalence variations among
different geographical areas and related diagnostic and therapeutic implications remain a major investigational
issue. We will focus on clinical and therapeutic implications of chlamydial infections in patients with
lymphomas and summarize the current knowledge on the association between Cp infection and OAMZL.
Available data on the epidemiology, biology and pathogenesis of this association are analyzed and new
investigative and clinical approaches are discussed.
Pag. 177 di 300 31/08/2011 ID nr. 4209
Luppi° A, Bonilauri° P, Mazzoni C, Spaggiari° B, Leonelli° R, Di_Lecce R, Dottori° M
Diagnostic approach about PMWS/PCVD and PCV2 infection evaluation in pig
Large Anim Rev. - Vol. 15 no 2 ( 2009). - p 55-60. - 13 bib ref
First author Luppi, Andrea
ID nr. 4211
Candotti° P, Rota_Nodari° S, Amadei A
The legislative decree 146/2001.
Suinicoltore. - Vol. 16 suppl 1 ( 2009). - p 21-28
First author Candotti, Paolo
The author illustrate the content of the legislative decree 146/2001 on the welfare of the animals kept for
farming purposes.
ID nr. 4212
Ducatez MF, Moreno_Martin° A, Ademola A. Owoade AA, Olatoye IO, Alkali BR, Maikano
I, Chantal J, Snoeck JS, Sausy A, Cordioli° P, Muller CP
Characterization of a new genotype and serotype of infectious bronchitis virus in Western Africa
J Gen Virol. - Vol. 90 ( 2009). - p 2679-2685. - 42 bib ref
Impact factor: 3,092
Between 2002 and 2007, more than 1000 chickens from commercial farms, live bird markets and backyard
farms in Nigeria and Niger were tested for the presence of the infectious bronchitis virus (IBV) genome.
Phylogenetic analysis of full-length sequences of the spike 1 (S1) gene revealed a new genotype of IBV that
we refer to as ‘IBADAN’. The minimum genetic distance to the closest ‘non-IBADAN’ strains (UK/7/93 at the
nucleotide level; H120 and M41 at the amino acid level) reached 24 and 32 % at the nucleotide and amino
acid levels, respectively. The full genome of the IBADAN reference strain (NGA/A116E7/2006) had a genetic
distance of 9.7–16.4 % at the nucleotide level with all available fully sequenced strains. As IBV S1 plays a
major role in antigenicity, the antigenic relatedness of NGA/A116E7/2006 was compared with strains of other
serotypes. NGA/A116E7/2006 did not cross-react with antisera against IT02, M41, D274, Connecticut or
793/B strains in virus neutralization assays. NGA/A116E7/2006 cross-reacted with the QX-like strain
ITA/90254/2005 but only to a low level (antigenic relatedness of 33 %), suggesting that IBADAN also
represents a new serotype. A comparison of S1 sequences identified several amino acids that may play a role
in IBV antigenicity. Despite the absence of obvious clinical signs in poultry infected by IBADAN strains, it is
important to test the cross-protection of current vaccine strains.
ID nr. 4213
Piccirillo A, Pasotto D, Moreno_Martin° A, Cordioli° P
Serological survey for influenza type A viruses in domestic dogs (Canis lupus familiaris) and cats
(Felis catus) in North-Eastern Italy
Zoonoses Public Health. - Vol. 2009). - p 1-5. - 31 bib ref
Impact factor: 1,333
To ascertain the potential transmission of influenza A viruses to dogs and cats, a serological survey was
carried out in North-eastern Italy. In a 4-year period, 637 serum samples were screened using a Mab-based
Pag. 178 di 300 31/08/2011 competitive ELISA for anti-nucleoprotein A (NPA) antibody detection of influenza viruses. No evidence of antiNPA antibodies was observed.
ID nr. 4214
Moreno_Martin° A, Barbieri° I, Sozzi° E, Luppi° A, Lelli° D, Lombardi° G, Zanoni° MG,
Cordioli° P
Novel swine influenza virus subtype H3N1 in Italy
Vet Microbiol. - Vol. 138 ( 2009). - p 361-367. - 34 bib ref
Impact factor: 2,370
First author Moreno_Martin, Ana
To date, three subtypes of swine influenza viruses, H1N1, H1N2, and H3N2 have been isolated in Italy. In
2006, a novel swine influenza virus subtype (H3N1) was isolated from coughing pigs. RT-PCR performed on
lung tissues, experimental infection in pigs with the novel isolate, and cloning the virus by plaque assay
confirmed this unique H and N combination. The novel isolate was also antigenically and genetically
characterized. Genetic and phylogenetic analysis showed that the complete HA gene of the H3N1 strain has
the highest nucleotide identity to three Italian H3N2 strains, one isolated in 2001 and two in 2004, whereas the
full length NA sequence is closely related to three H1N1 subtype viruses isolated in Italy in 2004. The
remaining genes are also closely related to respective genes found in H1N1 and H3N2 SIVs currently
circulating in Italy. This suggests that the novel SIV could be a reassortant between the H3N2 and H1N1 SIVs
circulating in Italy.
ID nr. 4215
Frasnelli° M, Raffini° E, Pongolini S, Zanotti S, Gallegati P, Savorellli P
E. coli O157 and thermotolerant campylobacter spp, detection in dairy cattle farms producing raw
milk in Ravenna province (italy)
Large Anim Rev. - Vol. 15 no 5 ( 2009). - p 199-203. - 28 bib ref
First author Frasnelli, Matteo
We carried out a study in order to find Escherichia coli 0157 and thermotolerant Campylobacter spp, and to
determine their prevalence in dairy cattle farms producing raw milk in Ravenna province (Italy). During 2008
summer months we collected and examined pooled recto-anal swabs, bulk milk samples and milking machine
filters using immunomagnetic separation for E. coli 0157 detection and microbiological standard methods for
thermotolerant Campylobacter spp, detection. A genetic characterization of all the E. coli 0157 strains we
isolated was made. E. coli 0157 prevalence was 33% in farms and 4.9% in pooled swabs. Thermotolerant
Campylobacter spp. prevalence was 83% in farms and 42% in pooled swabs. All E. coli 0157 strains we
isolated were positive for ease gene and for vero cytotoxins-coding genes. All bulk milk and milking machine
filters samples were negative for 'both pathogens. We think that the lack of E. coli 0157 and thermotolerant
Campylobacter spp, detection in bulk milk may be an index of good hygiene practice during milking
procedures, milk collection and storage. We observe that the farms found positive for E. coli 0157 in our study
were those located on the hills and where heifers used to graze during spring and summer months. We
confirm rectoanal swabbing as a valid sampling method for E. coli 0157 detection.
ID nr. 4216
Nigrelli° AD, Bertoletti° I, Boldini° M, Fabbi° M, Luini° M, Paterlini° F, Faccini° S
Prevalence of BVDV infection in dairy cattle in Lombardy and proposal of a farm eradication program
Large Anim Rev. - Vol. 15 ( 2009). - p 195-198. - 27 bib ref
Pag. 179 di 300 31/08/2011 Project PRC2004015 - Evaluation of BVD virus infection in dairy farms of Lombardy Region and definition of
a herd eradication programme. - Nigrelli Dr. Arrigo - Solo - MINSAN - 2004 - 155000,00 EUR 01/07/2005 0.00.00 - 31/12/2007 0.00.00 - 1 - M6 - Mantova - Nigrelli Dr. Arrigo - I
2 - M1 - Brescia - Alborali Dr. Loris - I
3 - M4 - Cremona - Boldini Dr. Massimo - I
4 - M8 - Pavia - Fabbi Dr. Massimo - I
5 - M5 - Lodi - Luini Dr. Mario - I
6 - M7 - Milano - Colmegna Dr.ssa Silvia - I
7 - M2 - Bergamo - Paterlini Dr. Franco - I
8 - M3 - Como-Varese-Lecco (Binago) - Sacchi Dr.ssa Cristina - I
First author Nigrelli, Arrigo Daniele
The authors have verified the seroprevalence of BVDV in a. stratified random sample of 240 cattle farms in
some provinces of Lombardy. In each herd, young (heifers) and adult (cows) animals were sampled and
tested for antibodies to BVDV NS 2-3 by ELISA. The prevalence of positivity in groups and the observed ODS
allowed to assess: (i) the presence of active circulation of BVDV in 89 herds (37.1%), (ii) responses related to
prior infection in 108 herds (45.0%) and (iii) responses probably related to the use of vaccine in 7 herds
(2.9%). Another sampling involved 194 farms located in two provinces of Lombardy in the course of 24
months, with quarterly examinations of the tank bulk milk antibodies by ELISA. The high percentage of
"seroconversion" (63 of 194 corresponding to 32.5%) strongly suggests the evidence for a high circulation of
BVDV among herds in Lombardy. The eradication protocol applied in 14 farms was based on the research of
BVDV by PCR of pools of 20 sera to cover the whole herd from one day old calves. Moreover all new-born
calves averse tested by PCR during the 9 following months. A minimum of 1 to a maximum of 14 (0.1% to
5.8%) persistently infected (PI) animals were identified in the controlled farms. The elimination of PI subjects
identified in all herds led to eradication of infection. This was documented by repeated negative antibody
response in 6/8 months old subjects and by PCR on the bulk milk samples.
ID nr. 4217
Fusaro A, Monne I, Cattoli G, De_Nardi R, Salviato A, Moreno_Martin° A, Capua I,
Terregino C
Gene segment reassortment between Eurasian and American clades of avian influenza virus in Italy
Arch Virol. - Vol. 155 ( 2010). - p 77-81. - 25 bib ref
Impact factor: 1,909
All genes of avian influenza A viruses are phylogenetically distinguished into two large clades, namely the
American and Eurasian clade. Reassortments among the gene segments of influenza viruses belonging to the
two distinct clades are rare events and have never been described in poultry in Europe and Asia before. This
study presents the genetic characterization of two influenza viruses isolated from domestic mallards in Italy in
2004 and 2005. Phylogenetic analysis of the entire genome showed that these viruses contain mixed gene
segments belonging to the American and Eurasian clades.
ID nr. 4218
Mattiello S, Andreoli E, Stefanelli A, Cantafora A, Bianchi° A
How to evaluate body conditions of red deer (Cervus elaphus) in an alpine environment
Ital J Anim Sci. - Vol. 8 no 4 ( 2009). - p 555-565. - 28 bib ref
Impact factor: 0,132
The aim of this investigation was to compare different indices for evaluating nutritional conditions of red deer
(Cervus elaphus) in an alpine environment during the autumn in order to detect the most convenient ones for
management purposes in our specific situation. Body conditions of 274 red deer were evaluated using kidney
fat index, back fat index and body condition scores. Body Condition Scores was the easiest but the least
Pag. 180 di 300 31/08/2011 reliable method. Both kidney fat index and back fat index were significantly affected by age and sex class
(always lower in younger animals) and, in females, also by lactation status. In stags, a negative regression
effect of culling date on both kidney fat index and back fat index was observed. A significant positive
correlation between kidney fat index and back fat index was recorded. Both kidney fat index and back fat
index were objective indicators of nutritional status and sensitive to changes in physical conditions, but back
fat index was both quicker and easier to be measured. As a direct implication, we suggest that back fat index
can be a practical and reliable indicator for monitoring red deer conditions in alpine areas during the autumn,
provided that the effects of sex, age and date of culling are taken into account.
ID nr. 4219
Battisti A, Franco A, Merialdi° G, Hasman H, Iurescia M, Lorenzetti R, Feltrin F, Zini M,
Aarestrup FM
Heterogeneity among methicillin-resistant Staphylococcus aureus from Italian pig finishing holdings
Vet Microbiol. - Vol. 142 ( 2010). - p 361-366. - 33 bib ref
Impact factor: 2,874
Project PRC2007106 - Prevalence study of methicillin-resistant S. aureus (MRSA) in fattening pigs - Battisti
dr. Antonio - UO - MINSAN - 2007 - 0,00 EUR - 29/09/2008 0.00.00 - 28/09/2010 0.00.00 - 2 N1 - Sezione diagnostica provinciale di Bologna - Merialdi Dr. Giuseppe - I
A survey for methicillin-resistant Staphylococcus aureus (MRSA) in finishing pig holdings was carried out in
Italy in 2008. MRSA isolates were characterised by spa-, MLST-, SCCmec- and antimicrobial susceptibility
typing. A prevalence of 38% (45/118, 95% CI 29.4–46.9%) positive holdings was observed. Eleven different
spa-types were found among 102 MRSA isolates, clustering in lineages associated with farm animals (ST398,
ST9, ST(CC)97 in 36 holdings) and humans (ST1, 7 holdings). Nine (7.6%) holdings were positive for two,
three or four different and unrelated spa-types in various combinations. ST398 was the most prevalent lineage
(33 positive holdings). The most prevalent spa-type was t899 (ST398), detected in 22 positive holdings. Three
novel spa-types (t4794 of ST9; t4795 of ST97; t4838 of ST398) were detected. Ten holdings were positive for
spa-type t1730, that proved to be a new single-locus variant of ST97, within the CC97 (ST1476). The most
prevalent SCCmec was Type V (79 isolates), while Type IVb was found in 10 isolates. None of the isolates
was positive for Panton-Valentine Leukocidin, while most of the t127 and t1730 isolates, one t4794, one
t4795, and one t2922 were positive for LukE-LukD genes. All 64 antimicrobial susceptibility tested isolates
were resistant to tetracyclines, with high resistance rates to trimethoprim (68.8%), erythromycin (60.9%), and
ciprofloxacin (35.4%). All t127, ST1 isolates were resistant to tetracycline–ciprofloxacin–erythromycin. This
survey provides the first report of MRSA ST1 and ST(CC)97 among pigs and the first report of MRSA ST9
from pigs in Europe. The presence of human-associated CA-MRSA (t127, ST1, SCCmec type V) in 6%
holdings surveyed can represent an additional MRSA reservoir for infections in humans..
ID nr. 4220
Finazzi° G, Miraglia° V, Daminelli° P, Monastero P, Bonometti° E, Gregorelli M, Boni° P
Listeria monocytogenes behaviour in gastronomy food: octopus and potato salad
Ind Aliment (Pinerolo). - Vol. 49 n 498 ( 2010). - p 28-34. - 5 bib ref
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Finazzi, Guido
Pag. 181 di 300 31/08/2011 Octopus and potato salad was artificially contaminated with a L. monocytogenes suspension (mix of three
different strains), packaged and stored at different temperatures 5°, 10°, 15° and 20°C for several days. This
food didn’t support the grow of Listeria: in fact during the shelf-life period we observed microorganism
decreasing with a speed directly correlated to storage temperature increasing. As a consequence, a batch of
octopus and potato salad respecting the critical limit for L. monocytogenes established by European
regulation for ready-to-eat foods (< 100 cfu/g) can be considered safe beyond the end of shelf-life period fixed
by the producer (9 days) not only at optimal storage conditions but also at domestic storage conditions or
even in situation of thermal abuse.
ID nr. 4223
Chiapponi° C, Zanni° I, Garbarino° C, Barigazzi° G, Foni° E
Comparison of the usefulness of the CACO-2 cell line with standard substrates for isolation of swine
influenza A viruses
J Virol Methods. - Vol. 163 ( 2010). - p 162-165. - 26 bib ref
Impact factor: 2,133
Project ESNIP2 - "European surveillance network for influenza in pigs 2 (ESNIP 2). - Van Reet dr.ssa
Kristien - UO - CE - 2005 - 300000,00 EUR - 01/01/2006 0.00.00 - 31/03/2009 0.00.00 - 4 - N5
- Parma - Foni Dr.ssa Emanuela - I
PRC2005016 - Epidemiologic surveillance of influenza virus circulation in domestic and wild animals:
detection, antigenic and genomic characterisation of viruses circulating in susceptible species of
domestic animals - Barigazzi Dr. Giuseppe - Solo - MINSAN - 2005 - 05/06/2006 0.00.00 04/06/2008 0.00.00 - 77500,00 EUR 1 - N5 - Parma - Foni Dr.ssa Emanuela - I
2 - N8 - Reggio Emilia - Dottori Dr. Michele - I
3 - N3 - Forlì - Massi Dr.ssa Paola - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
5 - M6 - Mantova - Nigrelli Dr. Arrigo - I
First author Chiapponi, Chiara
Influenza A virus isolation is undertaken routinely in embryonated chicken eggs, but to improve virus detection
various cell lines can be used. The CACO-2 cell line was compared to the MDCK cell line and embryonated
chicken eggs for the isolation of H1N1, H1N2, H3N2 swine influenza A virus subtypes from clinical specimens.
From 2006 to 2008, 104 influenza A samples found positive by PCR from 42 respiratory outbreaks in Italian
swine farms were examined by virus isolation. Sixty swine influenza A viruses were isolated (16 H1N1, 28
H1N2 and 16 H3N2) and their growth behaviour on the different substrates was examined. 16/16 H1N1, 28/28
H1N2 and 8/16 of H3N2 viruses were isolated from the CACO-2 cell line, while 7/16 H1N1, 3/28 H1N2 and
16/16 H3N2 viruses were isolated using embryonated chicken eggs. Only 9/16 H1N1, 1/28 H1N2 and 6/16
H3N2 viruses replicated in MDCK cells. A link was found between viral hemagglutinin and the isolation rate on
the various substrates. The CACO-2 line was statistically more sensitive (Fisher’s exact test, p < 0.01)
compared to the MDCK cells and embryonated chicken eggs for the isolation of H1N1 and H1N2 subtypes. In
contrast influenza A H3N2 virus was isolated more readily in embryonated chicken eggs than in cultured cells
(Fisher’s exact test, p < 0.01).
ID nr. 4226
Martinelli° N, Luppi° A, Lelli° D, Sozzi° E, Canelli° E, Moreno_Martin° A, Fontana° R,
Lavazza° A, Lombardi° G.
Prevalence of hepatitis e virus antibodies among pigs in Northern Italy
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 431-436. - 12 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
Pag. 182 di 300 31/08/2011 First author Martinelli, Nicola
The Hepatitis E virus (HEV), the causative agent of hepatitis E, is a non enveloped RNA virus, belonging to
genus Hepevirus, the only member of the Hepeviridae family. HEV is classified into four major genotype but
only one serotype is identifi ed. A survey to detect antibodies against hepatitis E virus was undertaken on 39
Italian pig farms (17 farrow to feeder, 10 farrow to fi nish and 12 fattening enterprises). For the study 1422 pig
sera samples were tested using a commercial indirect ELISA, originally developed for testing human sera and
properly adapted for the analysis of pig sera. 38 of the farms (97,43%) were positive for anti-HEV IgG
antibodies and 714 of 1422 sera samples resulted positive (50,21%). Th e study confi rm that HEV is spread
in pigs in Italy and is probably endemic in many farms.
ID nr. 4227
Tittarelli° C, Sozzi° E, Spaggiari° B, Alborali° GL, Cordioli° P, Lavazza° A
Electronmicroscopic diagnosis of swinepoxvirus, aetiological agent of sporadic skin disease during
the period 2002-2008 in Northern Italy
Atti Convegno SIPAS. - Vol. 35 ( 2009). - p 507-512. - 9 bib ref
Meeting Annuale della Societa Italiana di Patologia ed Allevamento dei Suini (SIPAS) (35 : Modena : 12-13
Marzo 2009)
First author Tittarelli, Cristiana
During the period 2002-2008, 44 skin samples were performed in northern Italy by electron microscopy (EM).
In 5 (11.3%) cases a diagnosis of suipoxvirus was ascertained, corfirming that the disease is sporadic. Of
these, 3 cases were piglets or weaning pigs, 2 were foetuses. Negative staining EM is a useful tool for
diagnosis of poxvirus infection in pigs.
ID nr. 4235
Bellini° S
General principles of biosecurity in farm and risk factors
La biosicurezza in veterinaria / edito a cura della Fondazione Iniziative Zooprofilattiche e Zootecniche. Brescia : Fondazione Iniziative Zooprofilattiche e Zootecniche, 2009. - (Atti delle giornate di Studio
Fondazione Iniziative Zooprofilattiche e Zootecniche ; 74) p 1-8. - 4 bib ref
First author Bellini, Silvia
Biosecurity is a set of preventive measures designed to reduce the risk of transmission of infectious diseases
in livestock. It assumes a transversal value and the implementation of a valid bio-security system represents
the key point in the management of the risk factors at fann leve]. Bio-security within a farming system should
be viewed as a whole as each part of the system is closely linked to the next. The knowledge of the
transmission pathway of an infectious agent is essential when selecting the most applicable control technique.
The paper reviews the main factors associated with the spread of infections, characteristics of pathogens,
hosts, environment and main pathways of infection.
ID nr. 4236
Miraglia° V, Finazzi° G, Daminelli° P, Bonometti° E, Gregorelli° M, Boni° P
Behaviour of Listeria monocytogenes in chunked or sliced seasoned Bresaola della Valtellina IGP
Ind Aliment (Pinerolo). - Vol. 48 n 496 ( 2009). - p 58-64. - 10 bib ref
First author Miraglia, Viviana
Pag. 183 di 300 31/08/2011 The purpose of this work was to get data of Listerio monocytogenes behaviour during the shelf-life of
portioned Tresco a della Valtellina IGP".At the end of the seasoning process, Bresaola has been
contominated with a m ixture of different L. monocytogenes stra i ns allowing to obtoin a surfoce concentrotion
of 10 03 ufc/cm2. Afterwords, different Bresaola were cut in pieces and vocuumpacked or sliced and pocked
in a mod ified otmosphere. Both products hove been preserved ot different temperatures in occordonce to the
shelf-life period estoblished by the producer. In this trial shelf-life was conducted ot 5°C, temperature shown
on the lobel of the product; ot 10°C, moderate heat abuse which corresponds to the temperature overoge of
the household preservotion; ot 15° and 20°C, heat abuse useful to define the product and/ or the pathogens
behaviour following the interruption of the cold choin. The experiment lasted 127 days for the chunked product
and 120 for the sliced one. It hos been verified and concluded that: with the bio-competition of the high lactic
flora percentage belonging to both products L. monocytogenes does not take roots in the product, but it
decreases during the shelf-life.
ID nr. 4237
Morandi F, Ostanello F, Fusaro L, Bacci B, Nigrelli° A, Alborali° L, Dottori° M, Vezzoli° F,
Barigazzi° G, Fiorentini° L, Sala V, Leotti G, Joisel F, Sarli G
Immunohistochemical detection of aetiological agents of proliferative and necrotizing pneumonia in
italian pigs
J Comp Pathol. - Vol. 142 ( 2010). - p 74-78. - 16 bib ref
Impact factor: 1,725
Proliferative and necrotizing pneumonia (PNP) is a form of interstitial pneumonia that occurs in weaning and
post-weaning pigs. PNP is characterized by hypertrophy and hyperplasia of type II pneumocytes and
coagulative necrosis and granular debris within alveolar spaces. Canadian and European studies suggest that
the porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are
the main causes of the disease, but Aujezsky's disease virus (ADV) and swine influenza virus (SIV) have also
been considered as potential aetiological agents. An immunohistochemical study was carried out on the lungs
of 28 Italian pigs with PNP in order to evaluate the role of PRRSV, PCV2 and ADV in PNP lesions. PRRSV
infection was identified in the lungs of 11 pigs, PCV2 in the lungs of four pigs and coinfection with both viruses
in the lungs of eight pigs. Neither virus was detected in the lungs of the remaining five pigs. ADV antigen was
not detected in any sample. The principle aetiological agent of PNP in Italy therefore appears to be PRRSV.
Coinfection with PRRSV and PCV2 is characterized by more severe microscopical changes in affected lungs.
ID nr. 4238
Strive T, Wright J, Kovaliski J, Botti° G, Capucci° L
The non-pathogenic Australian lagovirus RCV-A1 causes a prolonged infection and elicits partial
cross-protection to rabbit haemorrhagic disease virus
Virology. - Vol. 398 n 1 ( 2010). - p 125-134. - 42 bib ref
Impact factor: 3,042
Two caliciviruses occur in Australian wild rabbits: rabbit calicivirus Australia 1 (RCV-A1) and rabbit
haemorrhagic disease virus (RHDV), which is used in Australia as a biocontrol agent to reduce feral rabbit
populations. There is concern that RCV-A1 acts as a natural vaccine and protects from lethal RHDV infection.
To investigate this hypothesis, domestic rabbits were perorally infected with RCV-A1, monitored for 28 days
and subsequently challenged with RHDV. We show that RCV-A1 causes a non-pathogenic infection and is
shed in faeces for up to 7 days post-infection. RCV-A1 was detected in the bile 2 months post-inoculation,
indicating a prolonged or possible persistent infection. All animals infected with RCV-A1 developed antibodies
cross-reacting to RHDV. When challenged with RDHV, half of the rabbits (n = 4) survived the infection. The
results indicate that RCV-A1 is likely to persist in rabbit populations and can elicit partial cross-protection to
lethal RHDV infection.
Pag. 184 di 300 31/08/2011 ID nr. 4239
De_Nadai V, Finazzi° G, Daminelli° P, Bonometti° E, Bertolassi° R, Boni° P
Behavioural dynamics of several pathogen microorganisms during processing and ripening of
Bagòss cheese
Ind Aliment (Pinerolo). - Vol. 48 no 495 ( 2009). - p 58-63. - 13 bib ref
Project PRC2007001 - Determination of quality and safety features based on predictive microbiology and
risk assessment of traditional regional food products - Finazzi Dr. Guido CON DELIBERA DG N. 461
DEL 17/09/2010 SOSTITUITO DR. BONI CON DR. FINAZZI - Solo - MINSAN - 2007 - 615000,00
EUR - 01/09/2008 0.00.00 - 31/08/2010 0.00.00 - 1 - I3 - Reparto Microbiologia e Parassitologia
degli Alimenti e Sorveglianza Epidemiologica - Boni Dr. Paolo - I
2 - M6 - Sezione diagnostica provinciale di Mantova - Franzini Giuliana - I
3 - M9 - Sezione diagnostica provinciale di Sondrio - Bertoletti Dr.ssa Irene - I
4 - N1 - Sezione diagnostica provinciale di Bologna - Bardasi Dr.ssa Lia - I
5 - N6 - Sezione diagnostica provinciale di Piacenza - Cammi Dr.ssa Giuliana - I
6 - N7 - Sezione diagnostica provinciale di Ravenna (Lugo di Romagna) - Raffini Dr. ssa
Elisabetta - I
First author De_Nadai, Valentina
The aim of the survey was to value the behaviour of the most common dairy pathogens in the Bagòss, a
peculiar ripe cheese of the Bagolino area (Brescia). Milk wasn contaminated with a three strains suspension
of each of the investigated microorganism (L. monocytogenes, Salmonella typhimurium, E. coli O157:H7 e
Staph. aureus), then processed and ripened in collaboration with the dairy. Cooking and next rest under whey
of curd were not able to determine any effect on pathogens concentration. But rather the ripening ensured the
abatement of Salmonella, E. coli and Staph. aureus and the decrease of 4-5 log of Listeria concentration.
ID nr. 4241
Gasparini° M, Curatolo° M, Assini° W, Bozzoni° E, Tognoli° N, Dusi° G
Confirmatory method for the determination of nandrolone and trenbolone in urine samples using
immunoaffinity cleanup and liquid chromatography–tandem mass spectrometry
J Chromatogr A. - Vol. 1216 ( 2009). - p 8059-8066. - 30 bib ref
Impact factor: 3,756
First author Gasparini, Mara
A confirmatory method for the simultaneous determination of nandrolone (a and ß) and trenbolone (a and ß)
in urine samples by liquid chromatography electrospray mass spectrometry (LC–MS-MS) was developed.
After an enzymatic deconjugation, the urine was subjected to a one-step cleanup on a commercially available
immunoaffinity chromatography cartridge. The analytes were detected by liquid chromatography–positive ion
electrospray tandem mass spectrometry using deuterium labelled internal standards. The analytical procedure
was applicable to bovine and swine urine samples. The procedure was validated as a quantitative
confirmatory method according to the Commission Decision 2002/657/EC criteria. The results obtained
showed that the method was suitable for statutory residues testing regarding the following performance
characteristics: instrumental linearity, specificity, precision (repeatability and intra-laboratory reproducibility),
recovery, decision limit (CCa), detection capability (CCß) and ruggedness. The decision limits (CCa) obtained,
were between 0.54 and 0.60 µg L-1; the recovery was above 64% for all the analytes. Repeatability was
between 1.6% and 5.7% and within-laboratory reproducibility between 1.6% and 6.0% for all the steroids.
ID nr. 4242
Bassi° S, Carra° E , Carpana E, Paganelli° GL, Pongolini° S
A scientific note on the detection of spores of Paenibacillus larvae in naturally and artificially
Pag. 185 di 300 31/08/2011 contaminated honey: comparison of cultural and molecular methods
Apidologie. - Vol. 41 no 4 ( 2010). - p 425-427 - 11 bib ref
Impact factor: 1,493
Project PESTE AMERICANA - Development of diagnostic techniques and control systems for American
foulbrood of bees. - Carpana Dr. Emanuele - UO - ALTRI - 2009 - 14000,00 EUR - 01/01/2008
0.00.00 - 31/12/2010 0.00.00 - 1 - N4 - Sezione diagnostica provinciale di Modena - Bassi Dr.
Stefano - I
First author Bassi, Stefano
The detection of Paenibacillus larvae spores in honey produced by infected colonies enables an early
identification of American foulbrood (Ritter, 2003). Bacterial culture with colony counting is the reference
method for P. larvae spores assessment in honey (OIE, 2008), but it is time consuming and the overgrowth of
other Bacilli can confuse the results. The aim of our study was the evaluation of a PCR method targeting 16S
rDNA as a faster alternative to culture in the screening of honey samples for the presence of P. larvae spores.
The analytical sensitivity of both PCR and bacterial culture was determined. Then, PCR was compared to
culture to determine its ability to discriminate non-contaminated from contaminated samples with different
loads of spores One hundred and two samples were tested. They included 91 honeys from apicultural
production, 4 artificially-contaminated honey samples containing approximately 10,000 - 1,000 - 100 and 10
spores of P. larvae per gram, 1 spore-free honey , 6 water suspensions of P. larvae spores ranging from 104
to 10-1 spores/ml. The results indicate that the PCR protocol employed is as sensitive as culture with MYPGP
for P. larvae spores detection in hone. but it gives results in 24 hours instead of 8 days needed for bacterial
culture. The superior sensitivity of PCR for water suspensions compared to honeys could be due to PCR
inhibitors or less effective DNA extraction from honey.
ID nr. 4245
Amadori° M, Cristiano° A, Ferrari° M
Constitutive expression of interferons in swine leukocytes
Res Vet Sci. - Vol. 88 ( 2010). - p 64-71. - 50 bib ref
Impact factor: 1,345
First author Amadori, Massimo
Interferon (IFN)-a and IFN- positive cells were revealed by flow cytometry in peripheral blood mononuclear
cells (PBMC) of Specific Pathogen Free (SPF) pigs. A low prevalence of IFN- positive cells was also detected
in PBMC of some Porcine Reproductive and Respiratory Syndrome virus-infected pigs and uninfected, control
pigs. IFN-a positive cells showed phenotypes of both monocytes and plasmacytoid dendritic cells. The
presence of IFN-a in PBMC was also confirmed by Western blotting. By immunoprecipitation, IFN-a was
detected as 32 and 55–57 kDa bands in PBMC of healthy SPF piglets. These samples were also IFNpositive; the cytokine was revealed as 24, 37 and 54 kDa bands. The unusual molecular mass values of
intracellular interferons were probably due to oligomerization, as previously described for human IFN-a. Swine
intracellular IFN-a displayed the expected antiviral activity on bovine MDBK cells. The results indicate that
interferons are constitutively expressed in swine leukocytes with peculiar molecular features.
ID nr. 4246
Galarini R, Fioroni L, Moretti S, Gasparini° M, Dusi° G
Determination of nine corticosteroids in bovine urine using liquid chromatography tandem mass
spectrometry
1st Mass Spectometry Food Day : 2-3 December 2009 Parma / [Parma : s.n., 2009]. - p - 3 bib ref
Mass Spectometry Food Day (1 : Parma : 2-3 December 2009)
A liquid chromatography tandem mass spectrometry (LC-MS/MS) confirmatory method was developed for the
Pag. 186 di 300 31/08/2011 simultaneous determination of nine synthetic corticosteroids in bovine urines. After enzymatic hydrolysis, the
urine clean up was accomplished using a SPE column. The procedure was validated in accordance with the
criteria defined in Commission Decision 2002/657/EC.
ID nr. 4248
Olivo F, Gili M , Stella P, Dusi° G
Multi-residue method for detection of anticoccidial drugs in egg and muscle by liquid
chromatography - tandem mass spectrometry: validation according to Commission Decision
2002/657/EC
1st Mass Spectometry Food Day : 2-3 December 2009 Parma / [Parma : s.n., 2009]. - p - 2 bib ref
Mass Spectometry Food Day (1 : Parma : 2-3 December 2009)
A rapid and very effective analytical procedure for the liquid chromatographic detection of 8 coccidiostats in
muscle and eggs was developed and tested. The method validation study was performed according to criteria
defined by Decision 2002/657/EC for qualitative screening methods, and the following parameters were
determined: response linearity, specificity, CCß, ruggedness and stability. The proposed method is suitable
for screening analysis.
ID nr. 4249
Assini° W, Tognoli° N, Bozzoni° E, Gasparini° M, Dusi° G
Development and validation of a confirmatory method for the determination of gestagens in animal
fat sample by liquid chromatography-tandem mass spectrometry
1st Mass Spectometry Food Day : 2-3 December 2009 Parma / [Parma : s.n., 2009]. - p - 2 bib ref
Mass Spectometry Food Day (1 : Parma : 2-3 December 2009)
First author Assini, Walter
A confirmatory method for the determination of eight gestagens in animal fat was developed and validated.
After acetonitrile extraction and SPE clean up the analytes were detected by positive ion electrospray LCMS/MS using deuterium-labelled internal standards. The parameters taken into account during the validation,
carried out according to the Decision 2002/657/EC criteria, were: decision limit (CCa), detection capability
(CCb), precision, recovery, calibration curves, specificity and ruggedness.
ID nr. 4250
Massi° P, Tosi° G; Fiorentini° L
Experimental infection with the “IT-02” strain of avian Infectious Bronchitis virus in commercial
broilers vaccinated with different vaccination programmes using live attenuated vaccines
XVIth World Veterinary Poultry Association Congress : November 8-12, 2009 Marrakesh : book of abstracts /
[Marrakesh : s.n., 2009]. - p - 3 bib ref
World Veterinary Poultry Association Congress (16 : Marrakesh : November 8-12, 2009)
First author Massi, Paola
The trial was performed utilizing four groups (group 1 to group 4) of commercial broilers, reared in isolation
units in Italy at the IZSLER laboratory , Forlì. The aim was to evaluate the cross-protection induced by
different types of commercial Infectious Bronchitis (IB) live attenuated vaccines, heterologous with respect to
the challenge strain, and of vaccination programmes, against an experimental challenge with the “IT-02” strain
of avian Infectious Bronchitis virus (IBV IT-02). This strain was first isolated in Italy and then sequenced by
Bochkov, Y.A. and Drygin, V.V in 2002; it has been subsequently detected in several European countries.
Pag. 187 di 300 31/08/2011 Comparing a sequence of 343 basis pairs of the S1 gene, IT-02 virus showed 88.3%
homology with the 793/B variant and 77.4% with the Massachussets serotype. Group 1 was vaccinated at 1
and 14 days of age with a live vaccine (CR88 strain, Gallivac® IB88) belonging to the 793/B group of
IBV. Group 2 received one dose of a H120 IB live vaccine at day-old and one dose of the 793/B vaccine at 14
days of age. All of the vaccines were applied by eye-drop. Groups 3 (“positive controls”) and 4
(“negative controls”) were not vaccinated. Groups 1, 2 and 3 were experimentally challenged with an
infectious dose of 104DIE50 of IT-02 IBV strain, diluted in 0.1 ml of distilled water, and administered by
oculo-nasal route at 35 days of age. Group 4 was kept unvaccinated and unchallenged. The groups were
monitored during the 7 days post-challenge to assess vaccine protection by evaluating the following
parameters: clinical signs and gross lesions, level of antibodies to IBV detected by ELISA and ciliary motility of
tracheal epithelium. Ciliary motility is a natural mechanism of defence of the respiratory tract of animals
against external agents such as viruses and bacteria that could colonize the airways causing infections and
lesions in those tissues. Neither clinical signs nor lesions were caused by the IT-02 IBV challenge in the
vaccinated groups, whereas respiratory signs were observed in the group of positive controls. A serological
response was detected from 14 days of age in all of the vaccinated groups, with
the highest levels observed in Group 2 vaccinated with H120 at day old and 793/B at 14 days. The level of
protection afforded by vaccination, as measured by ciliary motility of tracheal rings 3 and 7 days postchallenge, proved to be above 95% in Group 2 which reported a protection index comparable to that of the
unchallenged controls (Group 4); it was above 80% in Group 1, whereas it was 0% in Group 3 (positive
controls). Overall these results indicate that cross-protection could be achieved by vaccination with
commercially available live IBV vaccines, against a novel strain of IBV, IT-02, that emerged in Europe during
the last few years. The highest protection was recorded in Group 2, vaccinated with H120 at dayold and 793/B
at 14 days of age.
ID nr. 4258
Carra° E, Taddei° R, Barbieri° I, Botti° G, Tranquillo° V, Iori A, Gibelli L, Cerioli° M,
Cavadini° P, Gelmetti° D, Pongolini° S, Capucci° L
Evaluation of three rapid diagnostic test used in bovine spongiform encephalopathy on 3 million
cows
III Workshop nazionale di virologia veterinaria : Istituto Superiore di Sanità, Facoltà di medicina veterinaria,
Università degli studi di Bari : Valenzano (Bari), 11-12 giugno 2009 : riassunti / a cura di Emiliana Falcone ...
[et al.]. - Roma : Istituto Superiore di Sanità, c2009. - (ISTISAN congressi ; 09/C4) p 30
Workshop Nazionale di virologia veterinaria (3. : Valenzano (Bari) : 11-12 giugno 2009)
First author Carra, Elena
In 2001 in Italy an active surveillance system was started to assess the prevalence of BSE in the cattle
population, based on the use of laboratory rapid tests.The aim of the current study was to report on the field
performances of 3 rapid tests: a western blot (WB), a chemiluminescence enzyme-linked immunosorbent
assay (ELISA), and an immunochromatographic assay, routinely used at 3 laboratories of the IZSLER over 8
years and on 3 million cows tested. The 3 tests proved to be reliable tools when applied to slaughtered
samples, showing no o very low false-positive, and low retesting frequencies. The performances of the
immunochromatographic assay, and especially the chemiluninescence ELISA were negatively affected,
resulting in higher false-positive and retesting rates on samples from fallen stock.
ID nr. 4262
Chiari° M, Zanoni° MG, Alborali° L, Salogni° C, Tittarelli C, Tagliabue° S, Fabbi° M,
Capucci° L, Lavazza° A
Application of a surveillance Program in Wild European Brown Hares (Lepus europeaus) in Brescia
province, North Italy
Atti del III Convegno Nazionale di Ecopatologia della Fauna Torino SIEF, 15-17 Ottobre 2009 / [s.l. : s.n.,
2009]. - p 14
Pag. 188 di 300 31/08/2011 Convegno Nazionale di Ecopatologia della Fauna Torino SIEF (3 : Torino : 15-17 Ottobre 2009)
First author Chiari, Mario
European brown hare is a game animal that undergoes to specific hunting management and restocking
programs. The progressive declining of the stability of hares’ populations in Europe has been associated,
among the other causes, to the occurrence of European Brown Hare Syndrome (EBHS). In mid ’90, the
serological checking of hares captured in closed zones (named ZRC), used for restocking of free-hunting
areas, represented the first application of a sanitary program. On hunting season 2006-07, a more completed
sanitary surveillance was adopted in Brescia Province. In addition to the control of hares captured in ZRC,
both the causes of death in free-living dead hares were determined and the internal organs of hares shot
during hunting were gathered and examined. Post-mortem examination and bacteriological, virological and
parasitological analysis as well as serological tests for EBHS, Brucella sp, Francisella tularensis, Leptospira
interrogans antibodies were carried out. Two types of serum sampling were used i.e. blood on paper from
open wound (shot hares) and liquid in the heart cavities (dead hares). In addition to the 252 sera taken in 7
different ZRC and the hares (31 carcasses and 150 shot) examined during 3 hunting season, 464 sera taken
from hares captured in ZRC during non-consecutive hunting seasons were examined. Laboratory results
indicate that EBHS is endemic in Brescia province and it is sporadically but constantly detected (diagnosed in
5 dead and 1 shot hares). A high seroprevalence with low level of mortality was found especially in high
density areas. Other diseases including zoonosis (brucellosis and tularemia) were never detected, but
Toxoplasma gondi was sporadically identified. The most common observed diseases were
pseudotubercolosis, pasteurellosis and parasitic infestations (coccidiosis, verminosis). The results of this
study firstly suggest that the deterministic model explaining the natural diffusion of EBHS could fit in the study
areas: where densities were higher, the virus could circulate stimulating hares immunity. Then, it should be
pointed that the application of surveillance programs is useful to ascertain the health status of hares and
represents an important part of that integrated hunting management based on the use of animals produced on
site within closed controlled zones for the restocking of hunting areas..
ID nr. 4263
Chiari° M, Lanfranchi° P, Zanoni° MG, Alborali° L, Salogni° C, Tittarelli° C, Tagliabue° S,
Fabbi° M, Lavazza° A
Application of a surveillance Program in Wild European Brown Hares (Lepus europeaus) in Brescia
province, North Italy
VI International Symposium on Wild Fauna : May 21-24, 2009 Paris, France : Atti / [s.l. : s.n., 2009]. - p 137
International Symposium on Wild Fauna (6 : Paris, France : May 21-24, 2009)
First author Chiari, Mario
The progressive decreasing in Europe of hare densities and the occurrence of epidemic diseases impose the
application of surveillance programs. This study focuses on the epidemiology of EBHS and other bacterial and
viral diseases in selected populations of free-living brown hares in Brescia, a province of North Italy. Either
serological as well as post mortem examination and bacteriological, virological and parasitological analysis
were carried out.
ID nr. 4264
Tittarelli° C, Gelmetti° D, Rota° Nodari S, Gibelli° L, Lavazza° A
Encephalitozoonosis of rabbits: the relationship between macro-microscopic kidney lesions and
antibody titers in rabbits at slaughterhouse
Atti delle Giornate di coniglicoltura ASIC 2009 : Forlì 2-3 Aprile 2009 / [s.l. : s.n., 2009]. - p 81-83. - 6 bib ref
Giornate di coniglicoltura ASIC : Forlì : 2-3 Aprile 2009)
Project RELO2004685CUNISVEZZ - Influence of weaning age and diet on productive performance,
prevention of digestive diseases and welfare of the young rabbit - Toschi Dr. Ivan - UO - REGIONE
Pag. 189 di 300 31/08/2011 - 2004 - 92817,00 EUR - 04/10/2004 0.00.00 - 31/01/2007 0.00.00 - 1 - G1 - Reparto di Virologia
e Sierologia Specializzata - Lavazza Dr. Antonio - I
PRC2005010 - Importance of blood sampling as investigation tool for the evaluation of health
condition and welfare of farming rabbits - Lavazza Dr. Antonio - Capofila - MINSAN - 2005 02/10/2006 0.00.00 - 02/05/2009 0.00.00 - 53000,00 EUR 1 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
2 - IZSUM - Perugini Dr. Gianni - E
3 - S.A.T.A. Servizio di Assistenza Tecnica Allevamenti - Sezione Conigli - Regione Lombardia Brivio Dr. Ruggero - E
First author Tittarelli, Cristiana
Encephalitozoonosis is a chronic parasitic infection caused by E. cuniculi, largely diffused in industrial rabbit
farms in Italy. From 107 meat or adult rabbits taken at slaughterhouse, we sampled the kidneys and the blood
in order to correlate macroscopic and microscopic lesions to anti-E.cuniculi antibodies determined using
Carbon Immono Assay (CIA) test. Over 85% of rabbits with lesions scoring from 1 to 4 resulted seropositive
whereas only 12% of the animals without kidney lesions were positive. A good correlation was found between
serological titers, ranging between 1/40 to 1/5120, and severity of lesions. The microscopic lesions reflected
the severity of the infection, thus making possible a graduation system on the base of the various changes
observed in the glomeruli and in the tubuli.
ID nr. 4265
Stacchiotti A, Pedretti N, Lavazza° A, Zanola A, Grigolato P, Aleo ME, Rezzani R
Nephrotoxic metals and stress response: a comparative in vitro study
Ital J Anat Embryol. - Vol. 114 no 2 suppl. 1 ( 2009). - p 208
Congresso Nazionale della Società Italiana di Anatomia e Istologia (63 : Torino : 10-12 September 2009)
Mercury Hg, lead Pb and cisplatin CisPt are nephrotoxic metals that affect S3 portion of cortical proximal
tubules. The unifying factor in determining their toxicity appears to be the generation of reactive oxygen ROS
and nitrogen species RNS that induces cellular defence mechanísm i.e. GSH, antioxidant enzymes, stress
proteins or may lead to cell-death by autophagy, apoptosis or necrosis. Autophagy is thought to be a general
response to stress contributing to cell death, but it may act as a cytoprotective mechanism. Stress proteins,
i.e. heat shock proteins HSPs, glucose regulated proteins GRPs and metallothioneins MTs, are cytoprotective
chaperones that stabilize other proteins or organelles damaged by heavy metals. This in vitro study was
performed to compare the effects of HgC12 1-40rnicroM, PbC12 10-500microM and CisPt 10-200microM, in a
rat proximal tubular celi line NRK52E exposed to each metal from 3h up to 48h. In partícular we focused on
the distribution and abundance of HSP72, HSP25, GRP75, GRP78, MTs and the expression of LC3-II a
marker of autophagy, by immunohistochemistry and immunoblotting. Concomitantly wc evaluated ROS/RNS
production and apoptosis/ necrosis by FACS or ultrastructural analysis.After 24h of treatment, 20 microM HgII
induced apoptosis/necrosis and ROS/RNS production that well correlateti with HSP72 and MTs induction. In
contrast, 60 or 300 microM PbII, evaluated at the same time, stimulated ER chaperone GRP78, but was
unable to induce HSP72, MTs, nor ROS/RNS. Remarkably, 10 microM CisPt early stimulated autophagy,
while, except for GRP78, stress proteins enhanced later at 24h following exposure to 50 microM CisPt,
concurrently with ROS, apoptosis and cytoskeleton damage. These results outline that, in NRK52E cell line,
the metal-induced stress chaperones are related to nephrotoxic mechanisms, such as oxidative damage and
apoptosis, but their expression changes as a function of each metal; in addition, the over-expression of ER
chaperone GRP78 appears to be associated to autophagy. So, the modulation of the stress response may
represent a complementary tool to better understand the specific mechanisms of meta] induced
nephrotoxicity..
ID nr. 4266
Corsetti G, Stacchiotti A, Filippini F, Lavazza° A, Rezzani R
Beneficial effects of amino acid supplementation in aged mice kidney
Pag. 190 di 300 31/08/2011 Ital J Anat Embryol. - Vol. 114 no 2 suppl. 1 ( 2009). - p 74
Congresso Nazionale della Società Italiana di Anatomia e Istologia (63 : Torino : 10-12 September 2009)
The kidney is particularly affected by age even when other pathology is absent, due to high requirement for an
active oxidative metabolism and regulation of blood pressure. It has been reported that balanced dici limited
aged-induced structural and metabolic changes in humans and experimental animals. This study was
performed to assess the efficacy of ora] amino acid AA mixture supplementation on the kidney of aged mice.
Methods: C573BL/6 mice were divided in two g~roups consisting of 12 months old controls treated with
standard rodent diet and tap water, and AA-group, administered AA mixture big-one 05, 1.5 g/kg daily in
drinking tap water for three months. Additionally 2 months old mice were used as adult physiological controls.
Fibrosis and collagen deposition were studied by Masson thrichrome and Sirius red polarized microscopy.
Moreover perfusion status of glomerulus and interstitial peritubular space was described by VEGF
imnnmohistochemistry. On semithin sections we measured the volume and density n°/100p2 of glomeruli and
the ratio between glomerular volume and total volume Vg/Vt. At ultrastructural leve], we evaluated the tubular
mitochondria distribution and density n°/100p2. Results Wc did not observe any difference in body weight
between AA feed and aged controls. Long term AA supplementation attenuates glomerular and interstitial
fibrosis, increases about 25% the Vg/Vt ratio 0.08±0.02 vs 0.10±0.03 eve if the density of glomeruli doesn't
change. The expression of cytochrome c-oxidase increased strongly in AA animals. In addition, tubular eNOS
immunostaining and VEGF pattern restricted to podocytes in aged mice, enhanced in glomerulus and
peritubular space after AA supplementation, suggesting an improvement of blood perfusion. Furthermore, the
Bc12 expression increased in AA group indicating the beneficial effects on tubular cells survival. The density
of mitochondria increased strongly in AA treated vs aged controls 15 ± 5.031100p2 vs 11.12 ± 3.02/100p2
and they vere distríbuted mainly near the basolateral side in proximal tubules. The results obtained from aged
mice supplemented with AA vere similar to adult controls. Conclusions: These preliminary data corroborate
the hypothesis, previously test-ed in muscles and heart, that a balanced nutritional intake might be crucial to
delay ageing changes in tbc kidney..
ID nr. 4267
Spaggiari° B, Rugna° G, Licata° E, Frasnelli° M, Barigazzi° G, Gelmini° L, Massi° P,
Renzi° M, Ricchi° M, Merialdi° G
Wildlife fauna monitoring program in emilia romagna: health status of roe deer (Capreolus
capreolus) population
Atti del III Convegno Nazionale di Ecopatologia della Fauna Torino SIEF, 15-17 Ottobre 2009 / [s.l. : s.n.,
2009]. - p 16
Convegno Nazionale di Ecopatologia della Fauna Torino SIEF (3 : Torino : 15-17 Ottobre 2009)
First author Spaggiari, Brunella
Roe deer (RD) was included in target species of Emilia Romagna monitoring program of wildlife during 20082009 with the aim of gathering information on population health status, prevalence of zoonotic agents and
relevant infectious diseases for interacting domestic livestock. The regional plan included serological
investigations for M. avium subsp. paratuberculosis (MAP), Brucella spp. and B. burgdorferi antibodies on
hunter-killed RD. Additional investigations for MAP, Brucella spp., VTEC, Y. enterocolitica, Salmonella spp.
and gastrointestinal parasites were performed on either found-dead (except for run-over subjects) or sick
(including unhealthy culled individuals) RD by bacteriological, biomolecular and parasitological analyses. Data
were evaluated by Fisher’s exact test (p<0.05). Overall 576 RD were examined: 464 hunter-killed and 112
either found-dead or sick. During post-mortem examination of carcasses or viscera, gross signs of
enterocolitis and diarrhoea were found in 14% of cases. Significantly, 55% of found-dead RD exhibited
diarrhoea while only 4% of hunted ones did. Serological investigations for Brucella spp. yielded negative
outcomes, while B. burgdorferi infection was found in 56/273 individuals. MAP antibodies were found in 4/353
healthy RD. On the other hand, MAP PCR-positive RD (7/35) were diarrhoic individuals. EAE gene+ E. coli
was detected 13/94 animals with statistically significant differences between shot and found-dead/sick RD.
Moreover, the pathogen was prevalent (p<0,05) in diarrhoic animals. Salmonella spp. was isolated from 2
non-diarrhoic culled RD. Eight percent of RD tested positive for Y. enterocolitica Biogroup1A, which includes
non-pathogenic european strains. Gastrointestinal strongyles occurred at high prevalence (46/131) even
Pag. 191 di 300 31/08/2011 though low parasite burdens prevailed. When present, low level coccidia parasitism (16/131) almost always
co-occurred with worms and rarely associated with diarrhoea. In the current survey pathogens typical of wild
ruminants were found to be associated with enterocolitis in RD. With respect to the investigated pathogens,
RD population does not represent an important source of zoonotic agents and its role in disease transmission
to livestock needs to be further investigated.
ID nr. 4268
Renzi° M, Buratti° L, Frasnelli° M, Rugna° G, Spaggiari° B, Merialdi° G
Serological survey on toxoplasma gondii diffusion in roe deer (Capreolus capreolus) in Emilia
Romagna
Atti del III Convegno Nazionale di Ecopatologia della Fauna Torino SIEF, 15-17 Ottobre 2009 / [s.l. : s.n.,
2009]. - p 33
Convegno Nazionale di Ecopatologia della Fauna Torino SIEF (3 : Torino : 15-17 Ottobre 2009)
First author Renzi, Maria
In Emilia Romagna roe deer (RD) (Capreolus capreolus) population has been constantly increasing during
recent years reaching high values of biotic density. The presence of Toxoplasma gondii in wild ruminants and
in other wild species was recognized since the early 1960s and RD is known to be an intermediate host of the
parasite. A seroprevalence study was performed during 2008-2009 to verify and quantify the diffusion of T.
gondii infection in RD populations of Emilia Romagna. In the present study the resulting prevalences are
presented and the efficacy of a multispecies ELISA assay is evaluated as an alternative to direct agglutination
test. RD sera were collected from Jan 2008 to Jul 2009 during Emilia Romagna regional wild fauna monitoring
plan and tested for T. gondii antibodies (Abs) by ELISA assay (ID-VET® commercial multispecies kit). Positive
sera were tested and confirmed by Indirect Immunofluorescence (IFI) (OIE method for sheep and goat) using
Biomerieux® slides and anti-RD IgG produced by IZSLER. Abs titers = 1:40 were considered positive.
Association was analyzed between prevalence data and risk factors such as of sex, province and age class (3
age classes: <11 mths; 12-23 mths; >23 mths) ( 2 test). Two hundred forty eight RD sera were examined and
63 tested positive, for an overall 25,4% prevalence (CI 95%: 20,1%-31,3%). Each ELISA-positive sample was
confirmed by IFI. In each province the prevalence was found to be as follows: Bologna 25,3% (CI 95%: 16,036,7), Forlì-Cesena 40% (CI 95%: 5,3-85,3), Modena 21,9% (CI 95%: 9,3-40,0), Ravenna 22,5% (CI 95%:
13,5-34,0), Reggio Emilia 29,2% (CI 95%: 18,6-41,8). No statistically significant differences were found for
different provinces and sex (p>0,05), while seroprevalence levels were significantly different in different age
classes (p<0,01): higher prevalences were found in individuals older than 23 mths (<12mths: 14,3%; 13mths23mth: 9,1%; >24mths: 32,06%). The prevalence found in the present study is medium-to-high if compared to
the prevalence found in some alpine areas and demonstrates the high and diffused exposure of adult roe deer
to Toxoplasma infection. Risk factors for toxoplasmosis transmission need to be further investigated. The
employed ELISA assay resulted to be specific and easy to use, representing an effective alternative to direct
agglutination test.
ID nr. 4269
Spaggiari° B, Gelmini° L, Fontana° MC, Lavazza° A, Merialdi° G
Diagnostic investigation on found-dead brown hares (Lepus europaeus) in three Emilia Romagna
provinces during 2008
Atti del III Convegno Nazionale di Ecopatologia della Fauna Torino SIEF, 15-17 Ottobre 2009 / [s.l. : s.n.,
2009]. - p 34
Convegno Nazionale di Ecopatologia della Fauna Torino SIEF (3 : Torino : 15-17 Ottobre 2009)
First author Spaggiari, Brunella
The aim of this work is to report the result of diagnostic investigations performed to ascertain the causes,
infectious or not, of death of wild brown hares (Lepus europaeus) found dead in the territory of three Emilia-
Pag. 192 di 300 31/08/2011 Romagna provinces (Reggio Emilia, Modena and Bologna). The carcasses of found-dead hares were
submitted to IZSLER laboratories for necropsy in order to establish the presence of infectious diseases and
identify the cause of death. Bacteriological, parasitological, virological (EBHS) investigations were performed
on all submitted subjects; cyto-histological and PCR methods (Toxoplasma gondii and Francisella tularensis)
were applied for further specific investigations. Overall 59 animals were examined. EBHSV antigen was found
in 4 hares showing typical lesions. Acute trauma was lethal for 5 individuals. Regarding bacterial and mycotic
infections, the following diagnoses were established: pasteurellosis (3), streptococcal infection (3),
pseudotuberculosis (2) and aspergillosis (2). As far as endoparasites are concerned: intestinal coccidiosis
(11), serosal/hepatic cysticercosis (10), parasitic broncopneumonia (Protostrongylus spp.) (8) and
toxoplasmosis (5) were diagnosed. Tick infestation was present in 4 hares. Finally, one hare was affected by
lymphoma and another one by proliferative hepatitis. Parasitic diseases were therefore the most frequently
detected ones (64%), followed by bacterial/mycotic diseases (17%), viral/neoplastic diseases (10%) and
trauma (8%). Intestinal coccidiosis was the most frequently diagnosed disease but also Cysticercus pisiformis
represented an interesting and relatively frequent finding. Fatal toxoplasmosis was established as the cause
of death in 8% of hares, which represents an index of the territorial pressure by definitive hosts (relevant
environmental contamination with oocysts). Two relevant zoonotic agents, Brucella spp. and Francisella
tularensis, were never detected. EBHSV, which indeed is endemic in Emilia Romagna since more than 20
years, was detected in a relative low number of hares, suggesting that this virus is not one of the most
common causes of death, as often occurs where the hares’ population have a large seroprevalencence. A
wide range of pathogens was found in the present investigation. Monitoring programs of wild population of
brown hare would be useful in order to better understand the epidemiology of different pathogens and their
impact on brown hare population health status
.
ID nr. 4270
Giammarioli M, Canelli° E, Ciulli S, Rossi E, De_Mia GM
Genetic diversity of bovine viral diarrhoea virus isolates from Italy
8th International Congress of Veterinary Virology : 23rd - 26th August 2009 Budapest - Hungary : 20 years of
ESVV: Integrating Classical and Molecular Virology : Programme & proceeding / [s.l : s.n., 2009]. - p 143 - 5
bib ref
International Congress of Veterinary Virology (8. : Budapest - Hungary : 23rd - 26th August 2009)
ID nr. 4271
De_Bernardis° F, Finazzi° G, Daminelli° P, Bertolassi° R, Boni° P, Bonometti° E, Boni° P
Dynamic of several pathogens in artificially contaminated Salame Piacentino DOP
VII workshop nazionale Enter-net Italia Sistema di sorveglianza delle infezioni enteriche Infezioni trasmesse
da alimenti e acqua : diagnostica ed epidemiologia : 4-5 novembre 2009 Roma / a cura di I Luzzi... [et al.]. Roma : Istituto Superiore di Sanità, 2009. - (ISTISAN congressi ; 09/C10) p 30
Workshop nazionale Enter-net Italia (7 : Roma : 4-5 novembre 2009)
First author De_Bernardis, Federica
The aim of this trial was to value the dynamic of Salmonella typhimurium, L. monocytogenes and E. coli
O157:H7, during the seasoning of Salame Piacentino DOP. Different salami were artificially contaminated
during preparation with these pathogens and samples were collected during the seasoning period. Salmonella
typhimurium decreased with a linear trend characterized by decimal decreasing time (D) = 16 days e 20 hours
± 19 hours. E. coli O157:H7 decreased with a D = 13 days e 5 hours ± 13 hours. Decreasing of L.
monocytogenes observed was very slow, with a D = 112 days e 23 hours ± 32 days e 21 hours, even in
presence of Staph. carnosus used as a starter culture.
Pag. 193 di 300 31/08/2011 ID nr. 4272
De_Nadai° V, Finazzi° G, Daminelli° P, Bertolassi° R, Boni° P
Behavioural dynamics of several pathogen microorganisms during processing and ripening of
Bagòss cheese
VII workshop nazionale Enter-net Italia Sistema di sorveglianza delle infezioni enteriche Infezioni trasmesse
da alimenti e acqua : diagnostica ed epidemiologia : 4-5 novembre 2009 Roma / a cura di I Luzzi... [et al.]. Roma : Istituto Superiore di Sanità, 2009. - (ISTISAN congressi ; 09/C10) p 32
Workshop nazionale Enter-net Italia (7 : Roma : 4-5 novembre 2009)
Project PRC2007001 - Determination of quality and safety features based on predictive microbiology and
risk assessment of traditional regional food products - Finazzi Dr. Guido CON DELIBERA DG N. 461
DEL 17/09/2010 SOSTITUITO DR. BONI CON DR. FINAZZI - Solo - MINSAN - 2007 - 615000,00
EUR - 01/09/2008 0.00.00 - 31/08/2010 0.00.00 - 1 - I3 - Reparto Microbiologia e Parassitologia
degli Alimenti e Sorveglianza Epidemiologica - Boni Dr. Paolo - I
2 - M6 - Sezione diagnostica provinciale di Mantova - Franzini Giuliana - I
3 - M9 - Sezione diagnostica provinciale di Sondrio - Bertoletti Dr.ssa Irene - I
4 - N1 - Sezione diagnostica provinciale di Bologna - Bardasi Dr.ssa Lia - I
5 - N6 - Sezione diagnostica provinciale di Piacenza - Cammi Dr.ssa Giuliana - I
6 - N7 - Sezione diagnostica provinciale di Ravenna (Lugo di Romagna) - Raffini Dr. ssa
Elisabetta - I
First author De_Nadai, Valentina
The aim of the survey was to value the behaviour of the most common dairy pathogens in the Bagòss, a
peculiar ripe cheese of the Bagolino area (Brescia). Milk wasn contaminated with a three strains suspension
of each of the investigated microorganism (L. monocytogenes, Salmonella typhimurium, E. coli O157:H7 e
Staph. aureus), then processed and ripened in collaboration with the dairy. Cooking and next rest under whey
of curd were not able to determine any effect on pathogens concentration. But rather the ripening ensured the
abatement of Salmonella, E. coli and Staph. aureus and the decrease of 4-5 log of Listeria concentration.
ID nr. 4273
Oliverio° E, Finazzi° G, Daminelli° P, Ducoli° S, Costanzi C, Bonometti° E
Behaviour of enteroxigenic Staph. aureus in cow milk added with lactic starter culture
VII workshop nazionale Enter-net Italia Sistema di sorveglianza delle infezioni enteriche Infezioni trasmesse
da alimenti e acqua : diagnostica ed epidemiologia : 4-5 novembre 2009 Roma / a cura di I Luzzi... [et al.]. Roma : Istituto Superiore di Sanità, 2009. - (ISTISAN congressi ; 09/C10) p 52
Workshop nazionale Enter-net Italia (7 : Roma : 4-5 novembre 2009)
First author Oliverio, Elisa
Aim of this trial was to value effects of lactic flora on enterotoxigenic Staph. aureus in milk. Two batches of
UHT milk, one inoculated with 5% of yogurt, were contaminated with a pathogen concentration of 104- 105
ufc/ml; two batches of raw milk, one inoculated with 5% of yogurt were contaminated in the same way. Staph.
aureus population increased and produced the toxin within 28 hours in UHT milk without lactic flora added
stored at 20°C. In UHT milk with yogurt and in both raw milk samples pathogen concentration didn’t vary
without toxin production. Lactic flora naturally or artificially added to milk is able to inhibit Staph. aureus
replication and toxin production at 20°C.
ID nr. 4274
Miraglia° V, Finazzi° G, Daminelli° P, Monastero° P, Bertolassi° R, Todeschi° S, Boni° P
Check of histamin presence in Iseo lake Alosa fallax lacustris kept in oil and in-vitro evaluation
VII workshop nazionale Enter-net Italia Sistema di sorveglianza delle infezioni enteriche Infezioni trasmesse
da alimenti e acqua : diagnostica ed epidemiologia : 4-5 novembre 2009 Roma / a cura di I Luzzi... [et al.]. -
Pag. 194 di 300 31/08/2011 Roma : Istituto Superiore di Sanità, 2009. - (ISTISAN congressi ; 09/C10) p 49
Workshop nazionale Enter-net Italia (7 : Roma : 4-5 novembre 2009)
First author Miraglia, Viviana
Batches of Iseo lake Alosa fallax lacustris kept in oil coming from five different producers were analysed for
histamine presence. In 2 samples were found 568 and 101 ppm of histamin associated to Enterobacteraceae
concentration of 103-105 ufc/g. Hafnia alvei strains isolated from positive fishes were inoculated in Tuna fish
infusion broth and kept at 30°C for over 60 days. In vitro histamin production was observed, with increasing of
concentration, not only associated to the log phase of Hafnia but also during stationary and decreasing
phases.
ID nr. 4275
Brookes MB, Irvine RM, NunezN, Clifford D, Essen S, Brown JH, Van_Reeth K, KuntzSimon G, Loeffen L, Foni° E, Larsen L, Matrosovich M, Bublot M, Maldonado J, Beer M,
Cattoli G
Influenza A (H1N1) infection in pigs
Vet Rec. - Vol. 164 no 24 ( 2009). - p760-761. - 2 bib ref
Impact factor: 1,240
ID nr. 4276
Recordati C, Gualdi V, Craven M, Sala° L, Luini° M, Lanzoni A, Rishniw M, Simpson KW,
Scanziani E.
Spatial distribution of Helicobacter spp. in the gastrointestinal tract of dogs.
Helicobacter. - Vol. 14 ( 2009). - p 180-191. - 43 bib ref
Impact factor: 2,470
BACKGROUND: In dogs, the gastric Helicobacter spp. have been well studied, but there is little information
regarding the other parts of the alimentary system. We sought to determine the spatial distribution of
Helicobacter spp. in the gastrointestinal tract and the hepatobiliary system of dogs using culture-independent
methods. MATERIALS AND METHODS: Samples of stomach, duodenum, ileum, cecum, colon, pancreas,
liver, and bile from six dogs were evaluated for Helicobacter spp. by genus, gastric, and enterohepatic
Helicobacter spp. Polymerase chain reaction, 16S rRNA gene sequence analysis, immunohistochemistry, and
fluorescence in situ hybridization (FISH). RESULTS: In the stomach, Helicobacter spp. DNA was detected in
all six dogs, with H. bizzozeronii and H. felis identified by specific polymerase chain reaction. Helicobacter
organisms were localized within the surface mucus, the lumen of gastric glands, and inside parietal cells. The
small intestine harbored gastric and enterohepatic Helicobacter spp. DNA/antigen in low amounts. In the
cecum and colon, Helicobacter spp. DNA, with highest similarity to H. bilis/flexispira taxon 8, H. cinaedi, and
H. canis, was detected in all six dogs. Helicobacter organisms were localized at the mucosal surface and
within the crypts. Gastric Helicobacter spp. DNA was detected occasionally in the large intestine, but no
gastric Helicobacter spp. were present in clone libraries or detected by FISH. CONCLUSIONS: This study
demonstrates that in addition to the stomach, the large intestine of dogs is also abundantly colonized by
Helicobacter spp. Additional studies are necessary to investigate the association between enterohepatic
Helicobacter spp. and presence of intestinal inflammatory or proliferative disorders in dogs.
ID nr. 4277
Radaelli E, Luini° M, Domeneghini C, Loria GR, Recordati C, Radaelli P, Scanziani E.
Expression of class II major histocompatibility complex molecules in chronic pulmonary
Mycoplasma bovis infection in cattle
J Comp Pathol. - Vol. 140 ( 2009). - p 198-202. - 17 bib ref
Pag. 195 di 300 31/08/2011 Impact factor: 1,398
Pulmonary inflammation often results in expression of the class II major histocompatibility complex (MHCII) by
both professional antigen-presenting cells (APCs; histiocytes and lymphocytes) and non-professional APCs
(respiratory epithelium and endothelium). In this study lesions from 17 cases of bovine chronic pneumonia,
associated with Mycoplasma bovis infection, were examined immunohistochemically for M. bovis antigen and
MHCII expression. Ten cases of chronic necrosuppurative bronchopneumonia (NBP) were shown to be
characterized by abundant perinecrotic M. bovis antigen associated with scant MHCII expression by
degenerate leucocytes. Seven cases of chronic catarrhal bronchointerstitial pneumonia (CBP) showed
prominent MHCII expression by both professional APCs and respiratory epithelium, in the absence of
intralesional M. bovis immunolabelling. The results suggest that prominent MHCII expression by both
professional and non-professional APCs plays a role in the pathogenesis of M. bovis-induced CBP.
Conversely, the role of MHCII expression in necrosuppurative foci typical of M. bovis-associated NBP can be
considered negligible.
ID nr. 4278
Kuntz-Simon G, Kyriakis CS, Foni° E, Maldonado J, Loeffen W, Brown IH, Essen S,
Madec F, Matrosovich M, Bublot M, Chenchev I, Peiris M, Ólsen C, Van_Reeth K
The european surveillance network for influenza in pigs (ESNIP)
Third annual meeting Epizone : "Crossing borders" : 12-15 May 2009 Antalya, Turkey / [s.l. : s. n., 2009]. Annual meeting Epizone (3rd : Antalya, Turkey : 12-15 May, 2009)
Project ESNIP2 - "European surveillance network for influenza in pigs 2 (ESNIP 2). - Van Reet dr.ssa
Kristien - UO - CE - 2005 - 300000,00 EUR - 01/01/2006 0.00.00 - 31/03/2009 0.00.00 - 4 - N5
- Parma - Foni Dr.ssa Emanuela - I
Swine influenza is an important cause of acute respiratory disease in pigs and pigs are considered as an
intermediate host for the transmission of influenza viruses to humans. While surveillance networks for human,
equine and avian influenza have been established decades ago, surveillance for swine influenza has long
been neglected. The "European Surveillance Network for Influenza in Pigs 2" (ESNIP 2) was a co-ordination
action (SSPE-CT-2005-022749, January 2006 - March 2009) funded by the European Commission in the 6ch
Framework Research Programme. It maintained and expanded the surveillance network established during
ESNIP 1 (2001-2004) and aimed to improve our knowledge of the epidemiology and evolution of swine
influenza viruses (SIVs) in Europe. During the 3-year period of the project, virological and serological
surveillance have been conducted in parallel in six European countries. The data confirmed that SIVs of
H1N1, H3N2 and H1N2 subtypes are co-circulating among European pigs. Still, there were differences in the
prevalence of each subtype on regional or national levels, with little if any H3N2 activity in the UK or Brittany
(Frane). No major antigenic changes in the hemagglutinin proteins of each SIV subtype were detected.
However, novel reassortant viruses between the first generation 111N2 reassortants and avian-like swine
H1N1 viruses were occasionally detected in Italy and France. These data will be used to optimise the
diagnosis and control of swine influenza. European swine influenza researchers also started to liaise with
researchers in the US and Asia with the purpose to compare the epidemiology of swine influenza on different
continents. Furthermore, the ESNIP consortium has been working on improved methods for the serological
detection of avian influenza in pigs. These initiatives and interactions are consistent with improved pandemic
preparedness and planning for human influenza.
ID nr. 4279
Lelli° D, Canelli° E, Luppi° A, Moreno° AM, Sozzi° E, Lombardi° G, Cordioli° P
Comparison among different serological gE ELISA kits for Aujeszky's disease by testing sera from
experimental infections
3th ESVV Veterinary Herpesvirus Symposium, April 22-24, 2009 Greifswald - Insel Riems / [s.n. : s.l., 2009].
-
Pag. 196 di 300 31/08/2011 ESVV Veterinary Herpesvirus Symposium (3th : Greifswald - Insel Riems : April 22-24, 2009)
First author Canelli, Elena
The routine Aujeszky's disease diagnostic activity is based on the serological analysis, in particular on the
detection of gE antibodies (gE-Ab). These antibodies are important for differentiating infected animals from
those vaccinated with marker vaccines. Four monoclonal antibodies (MAbs) based gE competitive ELISA kits
were used in a comparative analysis of 116 sera obtained from naive or vaccinated pigs, after the challenge
with virulent 75D19 ADV strain. The compared-kits were: Ingenzim ADV SE® (Ingenasa); IDEXX ADV gp1O
(Idexx); Svanovir PRV-9E-Ab® (Svanova) and our home-made kit (IZSLER, Brescia). They were randomly
identified as kit 1; kit 2; kit 3 and kit 4. The 116 sera were obtained from forty-nine pigs (50 to 60 days old) that
were experimentally infected in a strict confined "level 3" animal,facility by applying six different protocols in
order to obtain sera with variable antibody titres. Results indicated that: a) pre-sera and sera sampled in the
first days post challenge (5-6 d PI) were negative to each one of the four kits; b) 7-13 d PI sera gave
discordant results, and only one kit correctly identified alt them as positive; c) sera obtained after the 13 d PI
were positive to alt kits. These data underlined that tests had similar characteristics except for the ability to
detect early infection antibodies. Therefore there were no significative differences among kits in the gE
detection in very early and post 13 d PI sera. Sensibility and specificity were calculated for alt kits. Kit 1 is the
most sensitive (90%); the specificity of all kits was 100%, demonstrating that repeated vaccinations did not
result in false positive sera and that the gE-Ab were only induced after infection. Furthermore, in order to
ascertain if the antibodies detected by the four different kits were directed towards the sure epitome or
different ones, we also tested with the four kits eleven gE specific MAbs, which are known to recognize three
different antigenical determinate. Obtained results revealed that alt the kits detected antibodies produced
against the same or dose/overlapping epitopes. These epitopes are highly immunogenic, but certainly the
possibility that pigs produce antibodies against other epitopes can not to be excluded. Therefore it would be
useful for the future to make available a confirmatory test, different from the routine used competitive ELISA
test, in particular at the end of an eradication plan, given that the presence of "singleton reactor" has been
demonstrated also for the Aujeszky's disease.
ID nr. 4280
Bulgari O, Campagnari M, Bolzoni° G, Gigliotti C, Caroli AM
Quantification of bovine milk total protease activity
Sci Tec Latt Casearia. - Vol. 60 no 5 ( 2009). - p 463-472. - 24 bib ref
Milk protein system exerts a crucial role from the point of view of both milk nutritional quality and technological
properties. It is a dynamic system: milk proteins synthesised by the mammary cells undergo the action of
proteolitic enzymes with important effects on milk quality. Milk protein enzymatic hydrolysis can favourably
affect flavour development and texture changes during cheese ripening. However, unwished proteolysis can
have a negative effect on both dairy product quality and shelf-life. A colorimetric method was used to quantify
total proteases in bovine herd and individual samples. The method, based on azocasein substrate, was
optimised and standardised for fitting laboratory conditions and analysed milk characteristics. It is a cheap and
easily applicable method and can be used for routine milk tests. A preliminary analysis was performed on 20
herd and 40 individual milk samples. The protease mean activity was 5.6 mU/mL (standard deviation =1.1
mU/mL; minimum value = 3.2 mU/mL; maximum value = 8.4 mU/mL).
ID nr. 4282
Formato G, Giacomelli A, Nisi F, Bassi° S, Pongolini° S, Carra° E, Saccares S
Sanitization of european foulbrood through different beekeeping pratice
41st "Apimondia" International Apicultural Congress : The bee, sentinel of the environment : 15-20
September 2009 Montepellier (France) / [Montepellier : s.l., 2009]. "Apimondia" International Apicultural Congress (41th : Montepellier (France) : 15-20 September 2009)
Pag. 197 di 300 31/08/2011 ID nr. 4283
Venturi L, Angelini P, Baldelli R, Bellini R. Calzolari° M, Borrini BM, Dottori° M, Poglayen
G, Rugna° G, Venturelli C, Martini E, Tamba° M
Surveillance on vector-borne diseases in Emilia-Romagna Region, Italy
Trop Med Int Health. - Vol. 14 suppl 2 ( 2009). - p 49
European Congress on Tropical Medicine and International Health : 6th Mediterranean Conference on
Migration and Travel Health : 1st : Verona, Italy : 6-10 September 2009)
Impact factor: 2,312
In recent years an increase in vector-borne disease activity has been recorded in different regions. The
epidemiology of such diseases is conditioned by complex interactions among environment, parasite, vector,
man and eventually animal – either domestic or wild – reservoir. Global warming together with the increase of
transport of goods, animals and people travels are impacting the risk that exotic species, both of insects
andparasites, enter and establish in new territories. For these reasons a regional surveillance program
focused on vectored diseases was implemented in Emilia-Romagna. The aim of the project is to create a
regional surveillance and risk assessment system based on a multidisciplinary network, with the capability of
collecting data about both vector populations dynamics, and possible presence of pathogens in vectors, men
and animals. It has therefore been constituted a working group, coordinated by the General Direction for
Health and Social Policy of the Emilia-Romagna Region, composed by physicians, veterinarians and
entomologists. Activities have been addressed to the activation of an entomological monitoring system for key
vectors, to the development of diagnostic procedures of selected zoonosis, to the predisposition of a training
program. Because it is endemic in Emilia-Romagna Region, Leishmaniasis has been selected to test the
system. After only two months from its constitution, the working group had to manage the Chikungunya
epidemic occurred in Ravenna and Cervia municipalities during summer of 2007. After only 15 days from the
reporting of the first human cases, it has been possible to perform the diagnosis, isolating the virus from the
vector (Aedes albopictus), to define a reporting system of clinical suspects and diagnostic procedures in man.
In the same time an intensive vector control program was carried out in infected areas. The timing
organization of a multidisciplinary network focused on vector diseases surveillance and control is producing
important positive output in terms of managing capacity of epidemic situation and risk assessment.
ID nr. 4284
Luppi° A, Fontana° MC, Galletti° E, Spaggiari° B, Maioli° G, Bonilauri° P, Dottori° M,
Trocchi V, Merialdi° G
Fatal toxoplasmosis in European brown hares (Lepus europaeus) in Northern Italy
VI International Symposium on Wild Fauna : May 21-24, 2009 Paris, France : Atti / [s.l. : s.n., 2009]. - p 157158. - 4 bib ref
International Symposium on Wild Fauna (6 : Paris, France : May 21-24, 2009)
First author Luppi, Andrea
Six cases of fatal acute toxoplasmosis were observed in hares in different areas of Emilia Romagna region, in
Northern Italy, during a period of four months. The most characteristic lesion in all hares was a severe
splenomegaly. Toxoplasmosis was diagnosed through cytological examination of spleen imprint sampled
slides, confirmed by PCR in all hares. The high incidence of acute fatal cases of toxoplasmosis in hares
agrees with the observations of other Authors (Gustafsson et al., 1997). For this reasons hares should be
considered exceptionally susceptible to primary Toxoplasma gondii infection. Epidemiology and prevalence of
T. gondii in hares in Northern Italy necds further investigations.nThe monitoring of the disease in hares could
be used to evaluate the level of environmental oocysts contamination in specific geographic areas.
ID nr. 4285
Magnino° S, Ferreri AJM, Ponzoni M Cangi MG, Pasini E, Govi S, Sacchi L, Pecciarini L,
Pag. 198 di 300 31/08/2011 RestiAG, Guidoboni M, Vicari° N, Doglioni C, Dolcetti R
Issues on the association between Chlamydophila psittaci infection and ocular adnexal lymphomas
1st europena meeting on animal chlamydioses and zoonotic aspects EMAC : June 14-16, 2009 Murcia,
Spain : Procedings / by Caro MR, Salinas J, Buendia AJ. - [s. n. : s. l., 2009]. - p 42. - 5 bib ref
Europen meeting on animal chlamydioses and zoonotic aspects (1st : Murcia, Spain : June 14-16, 2009)
First author Magnino, Simone
Several lines of evidence support the association between Chlamydophila psittaci (Cp), the agent of avian
chlamydiosis in birds and psittacosis-omithosis in humans, and ocular adnexal lymphoma of mucosaassociated lymphoid tissue (MALT)-type (OAML), an indolent malignancy involving conjunctiva, lachrymal
gland, orbital soft tissues, eyehd and lacrimai sac (1). PCR-based methods have allowed to detect Cp DNA in
the lymphoma samples of 80% of cases and in the peripheral blood mononuclear ce115 (PBMC) of 41% of
these patients (1); immunohistochemistry, immunofluorescence, and laser-capture microdissection-assisted
PCR of lymphoma biopsies have showed thai monocytes/macrophages are the carriers of Cp (2);
transmission electron microscopy has demonstrated the presence of Cp elementary bodies in the cytoplasm
of monocytes/macrophages within OAML specimens (2). Cp has been isolated in celi cultures from PBMC
and from conjunctival swabs of OAML patients (3); finally, the treatment of OAML patients with specific
antibiotics (doxycycline) leading to eradication of Cp has resulted in long-lasting lymphoma remissions in 65%
of patients (4). There is a wide variability in Cp prevalence in OAML patients among countries and among
different regions within the saure country (5). For example, the prevalence of Cp infection in OAML patients
has been found to be 75% and 75-80% in studies from South Korea and from Italy, and 54% and 47% in an
Austrian and a German study, respectively, while no association at ali was reported in studies from Japan and
some US regions. Such variability may reflect genuine geographical variations or methodological biases, e.g.
variations in PCR techniques and conditions. The establishment of animal models confirming the
lymphomagenic potential of Cp and the development of in vitro tests for assessing the ability of lymphocytes
from OAML patients to proliferate, when stimulated by chlamydial antigens, are now considered among the
highest research príorities in this field.
ID nr. 4286
Laroucau K, Vorimore F, Sachse K, Vretou E, Siarkou V, Hermann W, Magnino° S,
Rodolakis A, Bavoil PM
Identification of Chlamydophila abortus vaccine strain 1B by PCR-RFLP
1st europena meeting on animal chlamydioses and zoonotic aspects EMAC : June 14-16, 2009 Murcia,
Spain : Procedings / by Caro MR, Salinas J, Buendia AJ. - [s. n. : s. l., 2009]. - p 38. - 1 bib ref
Europen meeting on animal chlamydioses and zoonotic aspects (1st : Murcia, Spain : June 14-16, 2009)
Comparative genomic analysis of a wild-type strain of the ovine pathogen Chlamydophila abortus (strain AB7)
and its nitrosoguanidine-induced, temperature-sensitive, viruleneeattenuated live vaccine derivative (strain
1B) identified point mutations unique to the mutant (Burall et al., submitted). Based on these results,
mutations were further characterized to confirm the predieted acquisition or loss of restriction sites in the live
vaccine genomic DNA. Of the 10 investigated sites which contained confirmed point mutations (CAB153,
CAB175, CAB220, CAB281, CAB283, CAB308, CAB469, CAB622, CAB636, CAB648 referred in the S26/3
complete genome), three sites corresponding to the loss of a restriction site in the vaccine strain were
retained for further studies. PCR-restriction fragment length polymorphism (PCR-RFLP) analysis based on
restriction enzyme cleavage at these three genomic sites was applied to a large number of C. abortus
reference strains and field strains. Our results show that the three investigated mutations are specific to the
vaccine, and as such provide a novel, easy-to-use method for differentiating between the vaccine strain,
pathogenic strains and non-vaccine field isolates. Direct examination of clinical samples demonstrated that
these markers are suitable for direct typing.
ID nr. 4287
Pag. 199 di 300 31/08/2011 Vicari° N, Mandola ML, Barcucci E, Rizzo F, Bellotti° M, Magnino° S
Detection of Chlamydiaceae in tissues and swabs from wild birds sampled foi avian influenza
surveillance in 2008-2009 in Piedmont, Italy
1st europena meeting on animal chlamydioses and zoonotic aspects EMAC : June 14-16, 2009 Murcia,
Spain : Procedings / by Caro MR, Salinas J, Buendia AJ. - [s. n. : s. l., 2009]. - p 41. - 6 bib ref
Europen meeting on animal chlamydioses and zoonotic aspects (1st : Murcia, Spain : June 14-16, 2009)
First author Vicari, Nadia
BACKGROUND: Chlamydophila psittaci, the agent of avian chlamydiosis in companior birds and poultry, has
been allow detected in wild birds (1). We report here the preliminary results of an investigation for chlamydiae
carried out in wild birds that had been sample in the context of avian influenza surveillance. MATERIALS AND
METHODS: One hundred and twenty specimens collected in 2008-2009 from wild birds (mallards, swans,
raptors and pigeons) sampled for the surveillance for avian influenza in Piedmont region, northern Italy, were
examined for the presence of chlamydiae by a real-time PCR assay targeting the 23S rDNA of
Chlamydiaceae, as a screening test (2). Samples that yielded a positive reaction were subsequently tested
with a real-time PCR targeting the MOMP gene (ompA), employing a specific probe for C. psittaci (3). Two
conventional PCR assays targeting the 16S rDNA and the omp2 were also performed (4, 5). The amplicons
obtained were digested in a RFLP assay with Msel and with Alum, respectively, in order to identify the
chlamydial species involved. RESULTS: Nineteen samples (15.8 %) tested positive for Chlamydiaceae in the
real-time PCR targeting the 23S rDNA. No amplification was detected with the ompA real-time PCR specific
for C. psittaci. The RFLP-PCR assays gave conflicting results: the one targeting the 16S rDNA yielded a
restriction pattern which could be referred to C. abortus, while the other targeting omp2 showed a pattern that
did not match any recognised chlamydial species. A further real-time PCR specific for C. abortus ompA was
performed on the 19 samples but did not confirm the presence of C. abortus. CONCLUSIONS: The
percentage of positivity detected in this survey is similar to the one reported in a recent research carried out in
France, where 10.6% of samples were found positive for chlamydiae (6). Sequencing is under way in order to
further characterize the involved chlamydiae.
ID nr. 4288
Vicari° N, Laroucau K, Vorimore F, Barbieri° I, Sachse K, Hotzel H, Labalestra I, Magnino°
S
Molecular analysis of four chlamydial isolates from the intestine and cloacal swabs of feral pigeons
sampled in Milan and Ferrara, Italy
1st europena meeting on animal chlamydioses and zoonotic aspects EMAC : June 14-16, 2009 Murcia,
Spain : Procedings / by Caro MR, Salinas J, Buendia AJ. - [s. n. : s. l., 2009]. - p 27. - 8 bib ref
Europen meeting on animal chlamydioses and zoonotic aspects (1st : Murcia, Spain : June 14-16, 2009)
First author Vicari, Nadia
BACKGROUND: Chlamydophila psittaci, the agent of avian chlamydiosis, is widely distributed in feral pigeon
populations of several European countries (1). We report here the preliminary data on the molecular typing of
four chlamydial isolates from feral pigeons sampled in Milan and Ferrara, northern Italy. MATERIALS AND
METHODS: Chlamydial isolates were grown in cell culture from cloacal swabs (n=2) and from the intestine
(n=2) of feral pigeons of Milan and Ferrara, respectively. DNA from these isolates was analyzed using
Chlamydiaceae-specific real-time PCR (2), ArrayTube DNA micro array (3) and species-specific molecular
detection tools, i.e. real-time PCR (4) and MLVA (5). Furthermore, characterization based on the ompA (6)
and 16S rDNA (7) complete sequences was performed. Phylogenetic trees were constructed with
BioNumerics software (Applied Maths, Sint-Martens-Latem, Belgium) by comparing the obtained sequences
with a database that included representative sequences of all recognized Chaamydaa and Chlamydophila
species. RESULTS: Unexpectedly, DNA extracted from all four isolates tested positive only in
Chlamydiaceae-specific real-time PCR, but no signal was detected with any of the C. psittaci specific tools
(real-time PCR, MI-VA). ArrayTube DNA micro array testing indicated the presence of a new, so far
unclassified member of the genus Chlamydophila. Another real-time PCR specific for novel Chlamydophila
organisms recently isolated from poultry in France (8) was applied to the samples, but no signal was detected,
Pag. 200 di 300 31/08/2011 thus suggesting either high intraspecies diversity or the presence of another different novel chlamydial
organism. Sequencing of the ompA and 16S rDNA genes of these samples confirmed this hypothesis.
CONCLUSIONS: Molecular analysis performed on the four isolates shows that they do not cluster with known
chlamydial species. Further molecular analyses are under way for a more complete characterization of the
isolates.
ID nr. 4289
Scala A, Mula P, Giobbe M, Ferraretto Z, Tittarelli° C, Lavazza° A
A preliminary seroepidemiological survey of encephalitozoon cuniculi in rabbit farms Sardinia (Italy)
Parassitologia. - Vol. 50 supp. 1 ( 2008). - p 158
Congresso Nazionale della Societa' Italiana di Parassitologia (25. : Pisa : 18-21 giugno 2008)
Project PRC2005010 - Importance of blood sampling as investigation tool for the evaluation of health
condition and welfare of farming rabbits - Lavazza Dr. Antonio - Capofila - MINSAN - 2005 53000,00 EUR - 02/10/2006 0.00.00 - 02/05/2009 0.00.00 - 1 - G1 - Reparto di Virologia e
Sierologia Specializzata - Lavazza Dr. Antonio - I
2 - IZSUM - Perugini Dr. Gianni - E
3 - S.A.T.A. Servizio di Assistenza Tecnica Allevamenti - Sezione Conigli - Regione Lombardia Brivio Dr. Ruggero - E
ID nr. 4293
Carra° E, Bassi° S, Sarti M, Fontana MR, D'Incau° M, Lambertini A, Pongolini° A
Molecular investigation of a human Salmonella enterica subsp. enterica serovar Manhattan outbreak,
comparison between outbreak and collection isolates
V Workshop nazionale di epidemiologia veterinaria "L'epidemiologia veterinaria di fronte ai cambiamenti
naturali e sociali che influenzano la salute" : Torino, 10-11 Dicembre 2009 / a cura di Gaia Scavia ... [et al.]. Roma : Istituto Superiore di Sanità, c2009. - (ISTISAN congressi ; 09/C13) p 32-33
Workshop nazionale di epidemiologia veterinaria (5. : Torino : 10-11 Dicembre 2009)
First author Carra, Elena
Few human outbreaks caused by Salmonella enterica subsp. enterica serovar Manhattan have been
described and investigated by molecular tools. The aims of this study were:1) the molecular-epidemiological
investigation of a human Salmonella enterica subsp. enterica serovar Manhattan outbreak occurred in
Modena in the period May-July 2009; 2) the genetic comparison of 15 isolates from the outbreak with 21
strains of Salmonella Manhattan from the IZSLER’s collection isolated from different sources, over the period
2001 -2009. The genetic analysis performed by pulsed field gel elecrophoresis (PFGE) revealed the same
genotype (type A) for all the outbreak isolates. Among 21 isolates from the IZSLER’s collection, 6 genotypes
were identified, and type A resulted the most frequent.
ID nr. 4295
Boniotti° MB, Donati° C, Zanardi° G, Lollai S, Zanoni° M, Tagliabue° S, Avisani° D,
Pacciarini° M
Evaluation of MIRU-VNTR Stability
Fifth international M. bovis conference / [s.l. : s.n, 2009]. - p 116 (
International M. bovis conference (5th : Wellington, New Zeland : 25-28 August 2009)
First author Boniotti, Beatrice
Spoligotyping and MIRU-VNTR (mycobacterial interspersed repetitive unit-variable-number tandem repeat)
typing has become a major method for genotyping of M. bovis isolates. Epidemiological investigations
Pag. 201 di 300 31/08/2011 confirmed by the genetic profiles data can elucidate the sources of infection of new TB outbreaks. The genetic
structure of MIRU-VNTR makes them subject to gain or loss repeats and confers them a genetic
discrimination capacity. A source of variation can also be the reliability of locus amplification. However, in
order to trace transmission chains over time it is necessary to use sufficiently stable genetic markers
throughout the research period. Comparing genetic profiles you can sometimes find SLVs (Single locus
Variations) or DLVs (Double locus Variations) but the significance of a single mutation has not yet been
established nor if it's sufficient to say the isolates are different. In this study, we evaluated the stability of 19
MIRU-VNTR loti using a total of 79 isolates belonging to 2 outbreaks groups. The first group included 47
isolates coming from a small area of Sardinia, an Italian island of Mediterranean Sea. The isolates were
collected over a period of 7 years from 36 cattle herds and from wild boars living in the sure area. A11 the
isolates showed the sure Spoligotype and most of them showed the sure MIRU-VNTR profile. We found 5
SLVs in loti ETR B, ETR C, MIRU 16, MIRU 27 and 2 involving marker 3636, considered a hyper variable
locus. None of the isolates showed a DLV. The second group includes 25 isolates mostly coming from the
North of Italy. All the isolates showed a Spoligotype consistent with M.caprae and the sure MIRU-VNTR
profile except for an SLV involving MIRU 23 and for an unusual VNTR 3232 variability. In fact, PCR
amplification of VNTR 3232 locus worked very badly in this group with results that were difficult to interpret.
Moreover, this group was compared with 6 unrelated isolates with a very similar genetic profile. These isolates
always showed DLVs.
Our results suggest that when in a locus the number of repeats is high its amplification is unreliable and it's
necessary the exclusion of this locus during epidemiology investigations. In conclusion, most of the markers
considered in this study showed high stability and the presence of a single locus variation was not significant
to exclude correlation among the isolates.
ID nr. 4296
Zanardi° G, Zanoni° M, Gaffuri° A, Boniotti° B, Pacciarini° ML, Alborali° L
Tubereulosis transmission by Mycobacterium Bovis in goats
Fifth international M. bovis conference / [s.l. : s.n, 2009]. - p 134 (
International M. bovis conference (5th : Wellington, New Zeland : 25-28 August 2009)
First author Zanardi, Giorgio
We describe a tuberculosis outbreak caused by Mycobacterium bovis in a mixed herd of cattle and goats. This
report examines the transmission of the virus from cattle to goats, its maintenance in the latter and its reoccurrence in cattle of new introduction. In order to detect the infection, cattle and goats were tested by single
and/or comparative skin test, gamma interferon test and then investigated at the slaughterhouse for gross
lesions. Mycobacterial isolates were identified by biochemical and molecular tests. Spoligotyping and MIRUVNTR analysis were used to genotype M. bovis isolates. In 2006, tuberculosis was detected at the
slaughterhouse in a nine-month fattening steer with lesions in the tracheobronchial and mediastinal lymph
nodes. The following epidemiological inquiry on the herd of origin clinically suspected one goat in 35 of having
tuberculosis (anorexia, weight loss, chronic cough). At the slaughterhouse visible lesions were found in the
retropharyngeal, tracheobronchial, mediastinal and mesenterie lymph nodes, the liver and the lungs. The
intradermal comparative tuberculin test carried out on the remaming 34 goats showed 20 positive heads using
the standard bovine interpretation. At necroscopy, 15 goats were found positive for gross lesions. Gamma
interferon test detected 19 positive heads. Bacteriological culture and PCR from clinical samples confirmed
the presence of M. bovis in both goats and cattle. Spoligotyping and MIRU-VNTR identified the same genomic
profile found two years before in a 10-year-old dairy cow in the same herd. Stamping out was practiced on
three cattle present at that time, but 18 goats living alongside were not skin tested. In conclusion, the goats
maintained and transmitted the infection to the fattening steer of new introduction into the same farm. The
identical genomic profile of M. bovis demonstrated the transmission of tuberculosis from cattle to goats to
cattle. Tuberculin skin test remains the in vivo diagnostic method of reference; the concordance of the results
with gamma interferon test was very high. According to similar results reported by Crawshaw T. et al. (The
Veterinary Record, July 26, 2008) it is also appropriate to test goats reared in promiscuity with cattle in order
to detect the disease, control TB diffusion and avoid the risk of zoonotic M. bovis infection.
Pag. 202 di 300 31/08/2011 ID nr. 4297
Gaffuri° A, Boniotti° B, Sacchi° C, Bertoletti° I, Zanoni° MG, Pacciarini M
Tuberculosis control program in wildlife in Italy: strategies and results
Fifth international M. bovis conference / [s.l. : s.n, 2009]. - p 126 (
International M. bovis conference (5th : Wellington, New Zeland : 25-28 August 2009)
First author Gaffuri, Alessandra
The presence of the tuberculosis infection in wildlife is a common concern in many countries; monitoring
control programs have been implemented worldwide to study its epidemiology in free-living animals and to
prevent inter-species transmission. Our study describes the tuberculosis control program, that we carried out
in Northern Italy and its results. In this region most of herds have been officially TB free for the last 5 years but
the high density of wild ungulates led us to assess the eventual presence of a TB wildlife reservoir. Since the
end of 90's we have controlled wild boar population by inspection of head lymph nodes followed by cultural
and biological molecular tests on the samples showing lesions consistent with Tb infection. In the last two
years we have also monitored foxes, roe deer and red deer, other wild species susceptible of TB that share
the sure habitat with wild boar. The samples were collected by the hunters or official veterinaries during the
hunting season. In the last ten years we examined 4200 wild boar, 152 roe deer, 208 red deer and 53 fox
lymph nodes. We found macroscopic lesions consistent with T13 in 333 wild boar lymph nodes; 170 sample
were identified as M. microti and 10 as M. bovis by PCR (IS6110 and RPFLP Gyr13) but we isolated only 20
M. microti and 4 M. bovis strains. The isolated M. bovis strains have different genetic profiles to those found in
recent T13 outbreaks occurring in cattle in the same area. We didn't find any macroscopic lesions in the lymph
nodes of the other species tested, but 1 roe deer were identified by PCR as M. microti, 5 red deer were
identified by PCR as M. bovis ( 3) and M. microti (2) and 2 fox, were identified by PCR as M. bovis. As we
were unable to isolate those M. bovis strains, we could not perform molecular typing to assess any correlation
with outbreaks in livestock. The results of the control program show that M. bovis is seldom detected in wildlife
and does not represent a risk for domestic animals, while M. microti is often present in tuberculosis-like
lesions.
ID nr. 4298
Bortolotti L, Sabatini AG, Mutinelli F, Astuti M, Lavazza° A, Piro° R, Tesoriero D,
Medrzycki P, Sgolastras F, Porrini C
Spring honey bee losses in Italy
10th International Symposium ICP-BR Bee Protection Group, Hazards of Pesticides to Bees : Bucharest,
Romania, 8-10 October 2008 : Abstract / [s.l. : s.n., 2008]. - p 4148-152 - 7 bib ref
International Symposium ICP-BR Bee Protection Group (10th : Bucharest, Romania : 8-10 October 2008)
First author Lavazza, Antonio
Backrgound: During last years several cases of bee losses have been reported during the period of corn
sowing in different European countries. In Italy an institutional system for bee losses survey does not exist
and therefore some Italian regions decided to organise an official network to collect data and analyse dead
bee samples. Results: Collected data indicate that the higher number of bee losses events occurred in
intensively cultivated flat areas, located in the North of Italy, mainly during or after corn sowing. The chemical
analyses of dead bees revealed the presence of three neonicotinoid residues: imidacloprid was found in
25.7% of the sample, thiamethoxam in 2.8%, clothianidin in 25.7%, both imidacloprid and thiamethoxam in
4.7%. The visual examination and the virological analyses excluded pathological causes. Conclusion: The
spatial and temporal correlation between hive damages and corn sowing and the presence of residues of
active ingredients used for seed dressing (imidacloprid, thiamethoxam and clothianidin) in almost half of the
samples confirms the connection between spring mortality and the sowing of corn seed dressed with
neonicotinoids.
ID nr. 4299
Pag. 203 di 300 31/08/2011 Bano L, Drigo I, Bonci M, Ferro T, Bacchin C, Guolo A, Marcon B, Merialdi° G, Agnoletti F
Clostridium difficile survey in Italian piggeries using different diagnostic methods
6th Clostpath International Conference : Clostridia the impact of genomics on disease control : 12-23
October 2009 Roma / [s.l. : s.n., 2009]. - p 159
Clostpath International Conference (6th : Roma : 12-23 October 2009)
In order to investigate the role of Clostridium dfficile (CD) in swine enteritis outbreaks, 79 faecal samples, 30
intestinal contents and 12 rectal swabs avere collected in 31 different farms from pigs with an history of dianhoea. Samples were stratified by growing phase (suckling, post-weaning, growing, fattening). Each sample
was cultured in a selective medium for CD and the isolates were Mentified by a commercial biochemical panel
kit and by means of a species-specific PCR. Each isolate was tested by multiplex PCR to reveal the presente
of tcdA and tcdB genes éncoding for toxin A and toxin B respectively. The samples were screened for CD
toxins A and B by usìng a commercial ELISA. 26 intestinal contents and 73 faecal samples were tested by
Real-Time PCR to enumerate CD Colony Forming Units (CFU) per g of sample. CD was recovered from 27
samples and the highest prevalente was detected in suckling pigs (43.5%). Thirteen strains tested positive for
both tcdA and tcdB genes, one strain was tcdA-Itcd6-, whereas 13 resulted tcdA-ItcdB+, 28 samples resulted
positive by Real-Time PCR and the highest CD
amounts (10 -10 UFC) avere detected in samples that tested positive for toxins 27 samples positíve for CD.
This study highlights the involvement of CD in outbreaks of enteric disease in swine in Italian farms,
irrespective of age, even though the highest prevalente was recorded in suckling pigs. Furthermore the
enumeration of this enteric pathogen by Real-Time PCR coupled with ELISA toxin test provides a rapid and
accurate tool far the diagnosis of clostridiosis caused by CD.
ID nr. 4300
Rossi L, Lavazza° A, Lovari S, Dematteis A
Encounters between livestock and the Himalayan tahr (Hemitragus jemahicus) in the Sagarmatha
National Park, Nepal
V Granada from 10th to 14th November 2009 : Granada from 10th to 14th November 2009 / [s.l. : s.n.,
2009]. - p 310
World conference on mountain ungulates (5 : Granada, Spain : 10th to 14th November 2009)
The Himalayan tahr (Hemitragus jemlahicus) is the most representative angulated living m the Sagarmatha
National Park (SNP), Nepal. This wild caprin is the object of a renowned long-term ethological study, in the
fame of the CNR-leaded "Ev-K2 Project". Since the beginning of the 21th century, the formerly viable tahr
population of the SNP was shown to be declining. A likely explanation was the synchronous return of an
efficient predator, the Snow leopard (Uncia), however other causes were explored. The present short-term
study aimed to investigate: i) how frequent and close are spatial interactions between W= and local livestock
(cattle, yak and hybrids); ii) if these interactions may actually remit in the cross transmission of pathogens
known to limit the reproductive success of affected hosts. Observations were carried out in February-April
2007, during 52 fieldwork days. Most observations (that we organized in 10 morning and 10 afternoon
session, 20 different days overall) focused on a single tahr herd in. the proximity of Namche Bazaar, at
altitudes between 3.000 and 3.500 m. Other observations (a "convenience" sample) dealt with several groups
of tahr living in these main valleys of SNP, up to 4.300 m. Results confirmed the critical demographic situation
of tahm in the protected area, and demonstrated that local livestock and tahr are in obvious spatial contact
during late winter-early spring. The alternate use of most available pastures and, in parallel, the frequent
occurrence of short-distance encounters (maggiore 20 m, with minima of 2 m) were documented. In addition,
serum samples were obtained from 20 adult tabr (3-11 years old), 35 1ocal livestock, and 30 goats and sheep
raised at lower altitudes and moved to the study area just a few weeks before slaughtering. Exposure to ten
major infections of nrminants was investigateti: Brucellosis, Chlamydiosis, Toxoplasmosis, Q Fever, Foot &
Mouth Disease, Neosporosis, Pestivirosis, BHV 1, BRSV, BVDVBDV, BPIV 3. Remarkably, 35% of tahr, 77 %
of livestock and 31 % of goat sera tested positive to BHV 1, a pathogen whose main transmission route is
short= distance aerosol. Notwithstanding, results of the serommoy do not support the hypothesis that
livestock-derived infectious diseases mducmg hypo-fertihty, abortion or neonatal juvenile mortality are major
causes of the low reproductive success of tahr in the SNP.
Pag. 204 di 300 31/08/2011 ID nr. 4301
Palermo P, Robetto S, Gaffuri° A,Gavaudan S, Ferrantelli, Pasolli C, Petrella A, Pintore A,
Di Ventura M, Battisti A, Di Prisco F, Cantucci U, Orusa R
Wild animal diseases: the Italian surveillance net
VI International Symposium on Wild Fauna : May 21-24, 2009 Paris, France : Atti / [s.l. : s.n., 2009]. - p 173174
International Symposium on Wild Fauna (6 : Paris, France : May 21-24, 2009)
First author Gaffuri, Alessandra
ID nr. 4302
Spaggiari° B, Merialdi° G, Cuccurese A, Bonilauri° P, Aldrovandi A, Massirio I, Leonelli° R,
Dottori° M
Survey of pathogen diffusion in a Roe Deer (Capreolus capreolus) population affected by diarrhoea
and increased mortality in Northern Italy
VI International Symposium on Wild Fauna : May 21-24, 2009 Paris, France : Atti / [s.l. : s.n., 2009]. - p 153154. - 5 bib ref
International Symposium on Wild Fauna (6 : Paris, France : May 21-24, 2009)
First author Spaggiari, Brunella
A survey was conducted between 2007 and 2008 on free-living roe deers (Capreolus capreolus) in the
province of Reggio Emilia (Northem Italy) following a reported increased mortality in the population during
summer 2007. Overall 267 animals were recovered during selective culling programs and submitted to gross
pathological, bacteriological, parasitological, virological and molecular biology investigations. Enterocolitis was
found to be the preeminent cause contributing to roe deer wasting and death. Yersinia spp., E. coli EPEC and
endoparasites were diagnosed and signilicantly corretated to diarrhoea and mortality. High roe deer densities
rccorded by drive census on the province territory in 2007 and unfavourable environmental conditions are
likely to have concurred to the lethal outcome for a considerable number of animals.
ID nr. 4303
Maioli° G, Bonilauri° P, Merialdi° G, Spaggiari° B, Casini° C, Dottori° M
Preliminary data on host preference, mean intensity of host infestation and geographical distribution
of tick infesting wild hunted animals in two Italian regions
VI International Symposium on Wild Fauna : May 21-24, 2009 Paris, France : atti / [s.l. : s.n., 2009]. - p 135136. - 4 bib ref
International Symposium on Wild Fauna (6 : Paris, France : May 21-24, 2009)
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
First author Maioli, Giulia
The aim of this study was to fumish more data ori the Ixoxid fauna of wildlife coilected in Emilia Romagna and.
Lombardia, two regions located in the northern part of Italy, and to better understand ticks ecology. Ticks were
Pag. 205 di 300 31/08/2011 sampled from hunter-killed animal and identified. A total of 1361 ticks were collected from 206 animals. The
most prevalent species was Ixodes ricinus followed by Dermacentor marginatus. Mean tick intensity was
lower in roe deer (Capreolus capreolus) than in other species and there was no significant variation in tick
abundance among months. Ticks were found in ali. sampling months including December and January.
Further investigations are in progress to detect tick-borne pathogens circulating in wildlife environment.
ID nr. 4304
Pessina A, Bonomi A, Coccè V, Bernardo ME, Cometa AM, Ferrari° M, Sisto F, Cavicchini
L, Locatelli F
Assessment of human herpesvirus-6 infection in mesenchymal stromal cells ex vivo expanded for
clinical use
Transpl Infect Dis. - Vol. 11 no 6 ( 2009). - p 491-496. - 18 bib ref
Impact factor: 2,516
Project relopianoregionalesa - Regional Blood Plan 2006 “Adult staminal cells transplantation for cellular
replacement-therapy purposes” - Prof.Franco Locatelli - UO - REGIONE - 2006 - 1230000,00 EUR
- 10/08/2006 0.00.00 - 09/08/2008 0.00.00 - 8 - L1 - Reparto Substrati Cellulari - Ferrari Dr.ssa
Maura - I
Infection or reactivation of human herpesvirus (HHV)-6 represents a potentially serious complication (often
involving the central nervous system) in patients receiving either solid organ or hematopoietic stem cell
transplantation. The objective of this study was to assess the risk of HHV-6 infection/reactivation in
mesenchymal stromal cells (MSCs). MSCs are multipotent cells displaying immunomodulatory properties that
have been already successfully used in the clinical setting to enhance hematopoietic stem cell engraftment
and to treat steroid-refractory acute graft-versus-host disease. We analyzed 20 samples of ex vivo expanded
MSCs, at different passages of culture, isolated both from bone marrow and from umbilical cord blood.
Through Western blotting and immunocytochemistry techniques, we investigated the presence of the HHV-6
receptor (CD46) on cell surface, whereas the presence of HHV-6 DNA was evaluated by nested polymerase
chain reaction assay. All of the MSC samples tested were positive for the virus receptor (CD46), suggesting
their potential susceptibility to HHV-6. However, none of the MSC samples derived from cultures, performed in
the perspective of clinical use, was found to harbor HHV-6. This preliminary observation on a consistent
number of MSC samples, some of them tested at late in vitro passages, indicates a good safety profile of the
product in terms of HHV-6 contamination. Nevertheless, it remains important to set up in vitro experimental
models to study MSCs' susceptibility to HHV-6 (and HHV-7) infection, to verify their capacity to integrate the
virus into cellular DNA, and to investigate which experimental conditions are able to induce virus reactivation.
ID nr. 4305
Pozzato N, Arrigoni° N, Bonizzato S, Chiavegato M, Tondo A, Capello K
Field and interlaboratory evaluation of four commercial ELISA kits for M. avium subsp.
paratuberculosis antibody detection in bovine serum
Proocedings of the 10th International Colloqium on Paratuberculosis : August 9-14 2009 Minneapolis,
Minnesota / [Minneapolis, Minnesota : s.l., 2009]. - p 54
International Colloquium on Paratuberculosis (10th : Minneapolis, Minnesota : August 9-14, 2009)
Project PRC2007102 - Diagnostic performances of commercial ELISA kits for the detection of
Mycobacterium avium subsp. paratuberculosis antibodies in infected and negative herds in Northeastern Italy - Pozzato Dr. Nicola - UO - MINSAN - 2007 - 0,00 EUR - 01/05/2008 0.00.00 30/04/2009 0.00.00 - 1 - N6 - Sezione diagnostica provinciale di Piacenza - Arrigoni Dr.ssa Norma
-I
Detection of antibodies by ELISA is an important tool for Johne’s disease control. The goal ofthis work was to
evaluate four commercial ELISA tests. We determined diagnostic sensitivity and specificity on field samples
and the robustness of the kits in an interlaboratory trial. To assess diagnostic sensitivity and specificity, 92
Pag. 206 di 300 31/08/2011 sera from fecal culture positive animals at different level of excretion and 32 from negative herds were
analysed in one laboratory. All the kits demonstrated a specificity of 100.0%. Merging inconclusive and
positive results, test sensitivity was 71.7% for kit A and B and 67.4 for kit C and D. Among infected animals,
ELISA sensitivity did not varied significantly with Map excretion levels with all the kit used. For the
interlaboratory trial, 30 coded samples composed by eight replicates of one negative sample and two
replicates of 11 positive samples were selected and delivered with the kits to 10 laboratories throughout Italy.
All the participants tested each sample in duplicates. Decoded results were analysed for reproducibility within
and among laboratories and quantitative results were transformed into S/P values to compare analytical
results. Kit A gave 100% of the expected results and Kit B gave almost the same outcome: just one laboratory
obtained one inconclusive and one negative result in one replicate. Kit C gave the expected results for 9/11
positive samples and Kit D for 5/11 positive samples. Variations among replicates and laboratories were
obtained with the remaining positive samples for these two kits. Regarding the replicates on negative sample,
5 incorrect results distributed in three laboratories for kit C and one doubtful replicate in one laboratory for kit
D were detected. According to these results, two (A and B) of the four ELISA kits evaluated showed good
performances and reproducibility within and among laboratories.
ID nr. 4306
Arrigoni° N, Cammi° G, Belletti° GL
Persistence of Mycobacterium avium subsp. paratuberculosis (Map) in field dried hay fertilized with
bovine slurry from Map infected herds
Proocedings of the 10th International Colloqium on Paratuberculosis : August 9-14 2009 Minneapolis,
Minnesota / [Minneapolis, Minnesota : s.n., 2009]. - p 172-175. - 7 bib ref
International Colloquium on Paratuberculosis (10th : Minneapolis, Minnesota : August 9-14, 2009)
Project PRC2005003 - Use of bulk-milk for bovine paratuberculosis surveillance - Arrigoni Dr.ssa Norma Solo - MINSAN - 2005 - 30000,00 EUR - 02/05/2006 0.00.00 - 01/05/2008 0.00.00 - 1 - N6 Piacenza - Arrigoni Dr.ssa Norma - I
2 - N0 - CEREV - Tamba Dr. Marco - I
First author Arrigoni, Norma
Objective: Use of Map contaminated slurry or manure to fertilize crop fields is generally considered a risk
factor for the Map transmission, although data on survival of Map in crops are sparse. Therefore, the
persistence of Map on crops fertilized during the autumn-winter period with slurry or manure coming from Map
infected herds was studied. Methods: Ten Map infected herds with different prevalence levels of infection
were selected. Culture and PCR tests were performed on environmental samples collected from the infected
farms to assess the level of Map contamination. The same tests were performed on crop samples collected at
three different time-points: (1) on fresh hay before harvesting; (2) on hay after field drying; (3) on dried hay at
the beginning of its use for animal feeding Results: The environmental samples had massive presence of Map
in the manure and slurry used to fertilize the fields. The tests performed on the fresh hay samples, collected
before harvesting, showed a single positive result by PCR (10%) and were always negative by culture. The
hay samples collected after field drying and at the beginning of their use for animal feeding were always
negative in both culture and PCR. Conclusion: These results suggested that under the described conditions,
the contamination risk for field dried hay, although possible, is of limited importance for the spreading of
infection. On the other hand this must not be underestimated in uninfected herds purchasing forage.
ID nr. 4307
Ricchi° M, Taddei° R, Barbieri° I, Belletti° GL, Pacciarini° ML, Arrigoni° N
Typing of Mycobacterium avium subsp. paratuberculosis (MAP) strains isolated from different Italian
regions by four Variable-Number Tandem Repeat (VNTR) methods alone or in association
Proocedings of the 10th International Colloqium on Paratuberculosis : August 9-14 2009 Minneapolis,
Minnesota / [Minneapolis, Minnesota : s.l., 2009]. - p 60-63. - 10 bib ref
International Colloquium on Paratuberculosis (10th : Minneapolis, Minnesota : August 9-14, 2009)
Pag. 207 di 300 31/08/2011 Project PRC2008108 - Set-up and evaluation of new epitopes for the detection of Mycobacterium avium
subsp paratuberculosis both “in vivo” and “in vitro” - Cagiola Dr.ssa Monica - UO - MINSAN - 2008
- 15000,00 EUR - 01/06/2009 0.00.00 - 31/05/2011 0.00.00 - 1 - N6 - Sezione diagnostica
provinciale di Piacenza - Ricchi Dr. Matteo - I
First author Ricchi, Matteo
The control of Paratuberculosis requires the knowledge of the causative agent, Mycobacterium avium subsp.
paratuberculosis (MAP), both in terms of epidemiology and biodiversity within different strains. One of the
most widely used method for MAP typing is IS900 sequence restriction fragment length polymorphism.
However it is applicable only on cultivable strains, is technically demanding and has limited discriminatory
power. More recently, tandem-repeat PCR based methods overcame these problems and, at present, are
considered the emerging techniques for MAP typing. The aim of this study was to evaluate the discriminatory
power of four PCR typing methods. We selected ten different strains from various Italian regions. We used
MIRU (3 loci), VNTR-MIRU (7 loci), MLSSR (11 loci) andMLVA (5 loci) analysis to differentiate the bacterial
DNA. Both MIRU and VNTR-MIRU gave 2 clusters (DI 0.556), while MLVA and MLSSR gave respectively 5
(DI 0.667) and 9 (DI 0.978) clusters. The combinations gave different results: MIRU+MLVA and VNTRMIRU+MLVA gave 5 clusters (DI 0.806), MLSSR+MIRU or MLSSR+VNTR-MIRU did not increase the
discriminatory results of MLSSR alone (9 clusters); MLSSR+MLVA gave 10 clusters (maximum theoretic DI
value i.e. 1.00). Although a limited number of strains was used in this study, our data suggest that applying a
single analysis, MLSSR provides the highest DI. Moreover, MLSSR coupled with MLVA showed the best
discriminatory power. Finally, the combination between MIRU or VNTR-MIRU and MLVA enhanced the
indexes as compared to single analysis.
ID nr. 4308
Ricchi° M, Manini° F, Cammi° G, Donaghy J, Arrigoni° n
Comparison of four different PCR methods for the detection of Mycobacterium avium subsp.
paratuberculosis in milk
Proocedings of the 10th International Colloqium on Paratuberculosis : August 9-14 2009 Minneapolis,
Minnesota / [Minneapolis, Minnesota : s.l., 2009]. - p 56-59. - 6 bib ref
International Colloquium on Paratuberculosis (10th : Minneapolis, Minnesota : August 9-14, 2009)
Project PRC2005003 - Use of bulk-milk for bovine paratuberculosis surveillance - Arrigoni Dr.ssa Norma Solo - MINSAN - 2005 - 30000,00 EUR - 02/05/2006 0.00.00 - 01/05/2008 0.00.00 - 1 - N6 Piacenza - Arrigoni Dr.ssa Norma - I
2 - N0 - CEREV - Tamba Dr. Marco - I
First author Ricchi, Matteo
Emerging evidence suggests a role of Mycobacterium avium subs. paratuberculosis (MAP) in the
development of human pathologies like Crohn’s disease and type I diabetes. For this reason, the need for
rapid and robust tools to detect the presence of MAP in food is increasing. Polymerase Chain Reaction (PCR)
techniques are able to give rapid and specific results, but their sensitivity is generally lower than traditional
culture methods. The aim of this study was to compare four different PCR methods to detect MAP on cow
bulk milk samples collected from presumably infected herds. MAP DNA was extracted by Adiapure kit
(Adiagene, France). The PCR were: (a) IS900 Commercial end-point PCR (kit Adiavet, Adiagene, France); (b)
IS900 Nested PCR; (c) IS900 TaqMan Real time PCR; (d) f57 housemade Sybr Green Real Time PCR. Both
the commercial PCR (a) and the IS 900 Real Time PCR TaqMan (c) contained an internal amplification
control in order to discriminate between negative or inhibited samples. Out of 37 milk samples tested we
found only one positive sample (3%) using (a), (c) and (d) methods. The Nested PCR method (b) showed
eight positive samples (22%). Although we used bulk milk samples coming from presumably infected herds,
the prevalence of direct detection of MAP is low in all the “one round” PCR methods. As expected, the most
sensitive method is Nested PCR, althoug it remains difficult to apply as regards to possibility of cross
contaminations.
Pag. 208 di 300 31/08/2011 ID nr. 4312
Candotti° P, Rota_Nodari° S
Surgical and functional surgery in a lactating sow affected by vulva biting
Suis. - Vol. 62 ( 2009). - p 40-43. - 5 bib ref
First author Candotti, Paolo
In a farrow to finish herd with 300 breeding sows, a granparent Large White sow had her vulva bitten by her
piglets in the farrowing crate. The cannibalism induced anatomical changes consisting in a loss of most of the
vulva's labias and a subsequent closure of the interlabial sulcus resulting in a reduction of the opening to
about only 3 mm. The anatomical changes had functional consequences resulting in: an abnormal urinating
behaviour, consisting in pollakiuria. In order to re-establish a welfare condition and to allow a natural
farrowing, the animal underwent successfully a plastic surgery to enlarge the interlabial solcus.
ID nr. 4313
Defilippo° F, Caimi M, Calzolari° M, Bonilauri° P, Parco V, Fedeli P, Barbieri° I, Maioli° G,
Lelli° D, Lavazza° A, Fabbi° M, Dottori° M
Arboviral surveillance program on mosquitoes from "parco lombardo della valle del Ticino"
(Northern Italy)
The 5th European Mosquito Control Association Workshop EMCA : Monday 9th March Friday 13th March
2009 Turin, Italy / [s.l. : s. n., 2009]. European Mosquito Control Association Workshop (5th : Turin, Italy : Monday 9th March Friday 13th
March 2009)
First author Defilippo, Francesco
Recently, Italv was involved in two important outbreaks of mosquitoborne diseases; chikungunya and West
Nile. In 2008 a preliminary surveillance program in Ticino River Park to check the presene of arboviruses in
mosquitoes was conducted. From 11 July 2008 to 17 October 2008, a total of 15,732 specimen belonging
mainly to species Aedes vexans (24%), Culex pipiens (22%) and Anopheles maculipennis (4%) were
collected. Of this, 14,232 mosquitoes (114 pools) belonging to the species Ae. vexans, Cx. pipiens, An.
maculipennis and Ochlerotatus caspius vere tested with PCR. One pool of An. maculipennis was positive for
the presence of a Bunyavirus. The BLAST analysis shows the sequence of the amplified fragment to have a
maximum homology (95%) with BATAI virus (GeneBank: AB257762). Virus isolation was attempted using celi
culture (Vero, Bhk21, Rkl3, C6/C36) and embryonated eggs but no positive results were obtained.
ID nr. 4314
Calzolari° M, Bonilauri° P, Defilippo° F, Maioli G, Bellini R, Veronesi R, Albieri A, Angelini
P, Barbieri° I, Lelli° D, Lavazza° A, Tamba° M, Sambri V, Dottori° M
West Nile Virus surveillance in mosquitoes in Emilia-Romagna (Italy)
The 5th European Mosquito Control Association Workshop EMCA : Monday 9th March Friday -13th March
2009 Turin, Italy / [s.l. : s. n., 2009]. European Mosquito Control Association Workshop (5th : Turin, Italy : Monday 9th March Friday 13th
March 2009)
First author Calzolari, Mattia
An the summer 2008 a large epidemic of West Nile Fever (WN) occurred in three different Regions of
Northern Italy (Emilia-Romagna, Veneto, Lombardia), causing 32 diagnosed cases in horses and 4 in
humans. An active entomologica surveillance plan was started by the Emilia-Romagna Surveillance Group on
Vectorial Disease in 2007. In the 2008 season a total of 78 stations were aestivated by C02 baited traps in
Bologna and Ferrara provinces, 40 stations historically operating for mosquito density monitoring and 38
Pag. 209 di 300 31/08/2011 stations specifically positioned after the first evidence of disease in equine. Mosquitoes were pooled according
to date, location and species, grinded manually and tested with Flavivirus genus RT-PCR and with WNV Real
Time PCR. In total 38791 mosquitoes were analyzed, most of them belonging to the species Culex pipiens,
Ochlerotatus caspius, Aedes albopictus and Aedes vexans. Two pools of Cx. pipiens (one collected in Cona,
Ferrara province, on September 23`h, and the other collected in Argelato, Bologna province, on September 3
0th) resulted positive in PCR for the presence of RNA belonging to the Flavivirus genus and also for the
presence of WNV. Virus isolation was attempted starting from the two PCR positive pools by using different
cells culture (Vero, Bhk21, Rk13, C6/C36) and by inoculation of SPF chicken embryonated eggs but no WNV
grown was obtained. The sequence of the amplified fragments (part of NS5 gene) obtained from of the two
positive pools were identical and BLAST analysis showed a highest similarity with two isolates from the same
outbreak in Emilia-Romagna - one from magpie (Pica pica) (100% homology, FJ472945) and one from human
(99% homology, FJ472946). For a more accurate molecular characterization of WNV the complete sequence
of the viral genome was required and that was possible only with an appropriate amount of viral RNA. As the
isolation of virus in PCR positive pooled mosquitoes failed the determination of the whole genome sequence
of the virus was precluded. Nevertheless the partial sequences obtained supported the specificity WN-PCR
detections and were sufficient to preliminarily classify "V strains as belonging to lineage I. Direct detection of
WN from mosquito vector is a rare event and confirms the high viral activity in the survey area. The maximum
likelihood estimation (MLE) per 1000 mosquitoes obtained by grouping weekly homogeneous samples
together results 0.69 (CI 0.04-3.37) for the week of first positivity (22/09-28/09) and 1.82 (CI 0.11-8.82) for the
week of the second one (29/09-04/10)
.
ID nr. 4315
Calzolari° M, Bonilauri° P, Bellini R, Maioli G, Defilippo F, Albieri A, Barbieri° I, Tamba° M,
Martini E, Angelini° P, Dottori° M
Evidence of circulation of Usutu virus in mosquitoes in Emilia-Romagna region (Italy)
5th International Congress of Vector Ecology : 11-16 October 2009 Delek - Antalya - Turkey : Proceedings /
[s.l : s.n., 2009]. - p 169
International Congress of Vector Ecology (5. : Delek - Antalya - Turkey : 11-16 October 2009)
First author Calzolari, Mattia
Usutu virus (USUV) is a mosquito-borne Flavivirus of the Japanese eneephahtis group. Vertebrate hosts of
this virus are birds and ite is pathogenic for certain passeriformes birds and raptors; little is known about the
medical important of USUV; but no human cases were reported. The firs detection of USUV in Europe date
back to 200lin Vienna, where the virus caused the death of hundreds of wild birds, subsequent viral isolations
and serological survives pointed out the presence of USUV in different European country, including Italy. The
vectors of USUV are ornithophilic mosquitoes, mainly of genus Culex. After the 2008 Italian outbreak of West
Nile Virus (WNVI that involved Emilia-Romagna, Veneto and Lombardia regions, in 2009 an extensive
entomological surveillance was set up, with the first aim to monitor the presence of WNV even though the
presence of USUV and other flaviviruses was also checked. The survey area was part of the Pianura Padana,
near the River Po, the most important Italian floodplain, characterized by an intensive agriculture and animal
husbandry. Almost all floodplain areas present in the region were monitored. Sites were monitored on a
weekly or be-monthly basis starting from May 2009; mosquitoes were trapped using modified CDC traps
baited with C02 and gravid traps. Since the beginning of the monitoring up to September 9th 2009, about
150,000 mosquitoes were analyzed, most of them belonging to the species Culex pipiens, Aedes
(Ochierotatus) caspius and Aedes vexans. The survey is still going on at the time the present abstract is being
written. Mosquitoes were pooled according to date, location and species, with a maximum number of 200
individuals per pool, grinded manually and submitted to Trizol LS - RNA extraction. Pools were analyzed using
3 different PCR reactions: 1) traditional PCR for the detection of flavivirus (Scaramozzino et al. 2001); 2)
traditional PCR for the detection of USUV (Weissenbóck et al. 2004); 3) Real Time PCR for the detection of
WNV (Tang et al. 2006). At the end of the first week of September,17 out of 1,211 pools of mosquitoes result
positive to the presene of USUV, all but one pool were composed of Cx. pipiens mosquitoes. Interestingly,
one pool Ae. albopictus resulted positive to - USUV. A part of the USUV amplicons obtained by the Favivirus
PCR (partial' NS5. gene) was sequenced. The sequence analysis confirms that. 1) the PCR amplicons`
obtained were specie for USUV; 2) all the strains sequenced show the same sequence in NS5 gene; 3) the
Pag. 210 di 300 31/08/2011 highest BLAST scores were obtained with Usutu virus. isolated in Budapest and Usutu virus strain Vienna
2001. It is also important to notice that 5 mosquito pools tested positive both for USUV and WNV. This
evidence could be considered a proof of the contemporary circulation of WNV and USUV in the same territory.
Further experimental investigation is needed to better characterize the contemporary circulation of WNV and
USUV. Moreover; it would be interesting to establish whether the mosquitoes infected by USUV are less
sensitive or refractory to infection with WNV, or single mosquitoes could be the vectors of both viruses at the
same time.
ID nr. 4317
Finazzi° G, Daminelli° P, Santilli L, Norton A, Gregorelli° M, Boni° P
Listeria monocytogenes behaviour in gastronomy food: rice salad
Ind Aliment (Pinerolo). - Vol. 49 n 503 ( 2010). - p 32-37. - 4 bib ref
First author Finazzi, Guido
The aim of this survey was to evaluate the behaviour of Listeria monocytogenes during shelf-life of rice salad.
For this purpose food was contaminated with a suspension of different pathogen strains in order to obtain a
concentration pair to 102-103 cfu/g, then packed and stored at four different temperatures: 5°, 10°, 15°, 20°C.
The trial lasted 4 weeks, a period longer than the shelf-life established by the producer. The results show that
Listeria monocytogenes is not a risk for the consumer because its concentration decreases during the shelflife period at all different storage conditions.
ID nr. 4319
Zanola A, Aleo MF, Schena I, Lavazza° A, Grigolato P, Stacchiotti A
Autophagy and apoptosis in NRK-52E renal cells exposed to cisplatin
1st Microscopy Conference : first Joint Meeting of Dreilandertagung Multinational Congress on Microscopy :
Graz, Austria 30 August - 4 September 2009 / a cura di Maria Anna Pabst, Gunther Zellnig. - [Austria : s.n,
2009]. - v. 2: Life science. - p 81-82. - 5 bib ref
Microscopy Conference (1st : Graz, Austria : 30 August - 4 September 2009)
ID nr. 4320
Lavazza° A, Tittarelli° C, Cerioli° M, Alborali° GL, Cordioli° P
ImmunoElectronMicroscopy (IEM) detection of viral agents in diarrheic pigs during the period 20022008 in Northern Italy
1st Microscopy Conference : first Joint Meeting of Dreilandertagung Multinational Congress on Microscopy :
Graz, Austria 30 August - 4 September 2009 / a cura di Maria Anna Pabst, Gunther Zellnig. - [Austria : s.n,
2009]. - v. 2: Life science. - p 443-444. - 4 bib ref
Microscopy Conference (1st : Graz, Austria : 30 August - 4 September 2009)
First author Lavazza, Antonio
ID nr. 4321
Lavazza° A, Cerioli° M, Tittarelli° C, Cordioli° P
The use of convalescent sera in nsIEM for the detection of not suspected and/or new virla agents of
veterinary relevance
1st Microscopy Conference : first Joint Meeting of Dreilandertagung Multinational Congress on Microscopy :
Graz, Austria 30 August - 4 September 2009 / a cura di Maria Anna Pabst, Gunther Zellnig. - [Austria : s.n,
2009]. - v. 2: Life Sciences. - p 437-438. - 6 bib ref
Microscopy Conference (1st : Graz, Austria : 30 August - 4 September 2009)
Pag. 211 di 300 31/08/2011 First author Lavazza, Antonio
ID nr. 4322
Bianchi° A, Caslini C, Mattiello S
Time budget assessment in captive Roe Deer (Capreolus capreolus) as a potential tool for predicting
release success
VI International Symposium on Wild Fauna : May 21-24, 2009 Paris, France : Atti / [s.l. : s.n., 2009]. - 1 p. 5 bib ref
International Symposium on Wild Fauna (6th : Paris, France : May 21-24, 2009)
First author Bianchi, Alessandro
This aim of the presene study is to analyze daily activity rhythms of captive roe deer and to detect possible
sources of variation inducing behavioural modifications. Roe deer that had been artificially reared by humans
in a wildlife rehabilitation centre (n = 3) tended to be less reactive than roe deer reared into the wild froin their
dams and delivered to the rehabilitation centre when already adult (n = 3). Centre bred deer spent significantly
less time moving and alert, thus having more time to dedicate to feeding. These behavioural differences might
probably be used to discriminate between "imprinted" and "wild' animals in rehabilitation centres, especially
when the animal's origin is unknown. This would be useful for supporting decisional processes as to the
release into the wild of those individuals.
ID nr. 4323
Ferris NP, Nordengrahn A, Hutchings GH, Reid SM, King DP, Ebert K, Paton DJ,
Kristersson T, Brocchi° E, Grazioli° S, Merza M
Development and laboratory validation of a lateral flow device for the detection of foot-and-mouth
disease virus in clinical samples
J Virol Methods. - Vol. 155 ( 2009). - p. 10-17. - 21 bib ref
Impact factor: 2,077
Project 2003-SSP-3-513645 - New and emerging technologies: improved laboratory and on-site detection of
oie list a viruses in animals and animal products (LAB-ON.SITE) - Belak Dr. Sandor - UO - CE 2004 - 1500000,00 EUR - 01/11/2004 0.00.00 - 31/07/2008 0.00.00 - 7 - H1 - Reparto di
Biotecnologie - Brocchi Dr.ssa Emiliana - I
A lateral flow device (LFD) for the detection of all seven serotypes of foot-and-mouth disease virus (FMDV)
was developed using a monoclonal antibody (Mab 1F10) shown to be pan-reactive to FMDV strains of each
serotype by ELISA. The performance of the LFD was evaluated in the laboratory on suspensions of vesicular
epithelia (304 positive and 1003 negative samples) from suspected cases of vesicular disease collected from
86 countries between 1965 and 2008 and negative samples collected from healthy animals. The diagnostic
sensitivity of the LFD for FMDV was similar at 84% compared to 85% obtained by the reference method of
antigen ELISA, and the diagnostic specificity of the LFD was approximately 99% compared to 99.9% for the
ELISA. The device recognized FMDV strains of wide diversity of all seven serotypes but weaker reactions
were often evident with those of type SAT 2, several viruses of which were not detected. Reactions with the
viruses of swine vesicular disease and vesicular stomatitis that produce clinically indistinguishable syndromes
in pigs and cattle, did not occur. The test procedure was simple and rapid, and typically provided a result
within 1–10 min of sample addition. Simple homogenizers that could be used in field conditions for preparing
epithelial suspensions were demonstrated to be effective for LFD application. These data illustrate the
potential for the LFD to be used next to the animal in the pen-side diagnosis of FMD and for providing rapid
and objective support to veterinarians in their clinical judgment of the disease.
ID nr. 4324
Eldin P, Papon L, Oteiza A, Brocchi° E, Lawson TG, Mechti N
Pag. 212 di 300 31/08/2011 TRIM22 E3 ubiquitin ligase activity is required to mediate antiviral activity against
encephalomyocarditis virus
J Gen Virol. - Vol. 90 ( 2009). - p 536-545. - 44 bib ref
Impact factor: 3,092
The interferon (IFN) system is a major effector of the innate immunity that allows time for the subsequent
establishment of an adaptive immune response against a wide-range of pathogens. Their diverse biological
actions are thought to be mediated by the products of specific but usually overlapping sets of cellular genes
induced in the target cells. Ubiquitin ligase members of the tripartite motif (TRIM) protein family have emerged
as IFN-induced proteins involved in both innate and adaptive immunity. In this report, we provide evidence
that TRIM22 is a functional E3 ubiquitin ligase that is also ubiquitinated itself. We demonstrate that TRIM22
expression leads to a viral protection of HeLa cells against encephalomyocarditis virus infections. This effect
is dependent upon its E3 ubiquitinating activity, since no antiviral effect was observed in cells expressing a
TRIM22-deletion mutant defective in ubiquitinating activity. Consistent with this, TRIM22 interacts with the
viral 3C protease (3CPRO) and mediates its ubiquitination. Altogether, our findings demonstrate that TRIM22
E3 ubiquitin ligase activity represents a new antiviral pathway induced by IFN against picornaviruses.
ID nr. 4325
Papon L, Oteiza A, Imaizumi T, Kato H, Brocchi° E, Lawson TG, Akira S, Mechti N
The viral RNA recognition sensor RIG-I is degraded during encephalomyocarditis virus (EMCV)
infection
Virology. - Vol. 393 ( 2009). - p 311-318. - 50 bib ref
Impact factor: 3,539
RNA helicase-like receptors MDA-5 but not RIG-I has been shown to be essential for triggering innate
immune responses against picornaviruses. However, virus–host co-evolution has selected for viruses capable
of replicating despite host cells antiviral defences. In this report, we demonstrate that RIG-I is degraded during
encephalomyocarditis virus (EMCV) infection. This effect is mediated by both the viral-encoded 3C protease
and caspase proteinase. In addition, we show that RIG-I overexpression confers IFN-ß promoter activation
during EMCV infection, in MDA-5 knockout (MDA-5-/-) mouse embryo fibroblasts. This induction is followed by
a strong inhibition reflecting the ability of EMCV to disrupt RIG-I signalling. Taken together, our data strongly
suggest that during evolution RIG-I has been involved for triggering innate immune response to picornavirus
infections.
ID nr. 4326
Tamba° M, Bonilauri° P, Galletti° G, Bonazza V, Zanardi° G
Tracing activities on cattle coming from bluetongue restricted zones of north europe
Blutongue Satellite Symposium : "Blutongue in Europe back to the future" : 7 June, 2008 Brescia, Italy :
programme and abstracts / [s.l. : s. n., 2008]. - p 56
Blutongue Satellite Symposium : Brescia, Italy : 7 June, 2008)
First author Tamba, Marco
Introduction and objectives. Italy has been infected by Bluetongue virus (BTV) since 2000, but is still free from
BTV serotype 8, the BTV strain responsible of epidemic in North Europe. Animal movement is the main risk
factor related to the introduction of a new pathogen agent into a territory. Such risk is strongly associated to
number of imported animals and lack of active surveillance system in the exporting country. During the period
July-December 2007 an overall number of 1,630 batches - 57,556 heads - were introduced from BTV-8
resctricted zones into Lombardy and Emilia-Romagna Regions. Most of them came from France. In the
present work first results of tracing activities performed on imported cattle coming from North Europe are
reported. 2.Material and Methods During period October 2007–January 2008, 21,879 blood samples were
Pag. 213 di 300 31/08/2011 collected from imported cattle. A total of more 7,117 blood samples has been collected in repeated checks of
infected herds and monitoring of surrounding farms for identification of BTV-8 positive cattle. 3.Results 123
imported cattle (0.6%) resulted BTV-8 positive and more than 80% of seropositive samples were also PCR
positive. In herds with infected imported animals a limited number of animals sero-converted. No BTV-8
seropositive animals were identified in farms surrounding infected herds. 4.Discussion and Conclusions Data
collected show as BTV-8 has been introduced in Italy through import of cattle coming from infected zones.
ID nr. 4327
Tamba° M, Rugna° G, Galletti° G, Leonelli ° R, Luppi° A, Piccolomini L
Incidence survey in an area interested by active circulation of vaccinal strain of bluetongue virus
serotype 2
Blutongue Satellite Symposium : "Blutongue in Europe back to the future" : 7 June, 2008 Brescia, Italy :
programme and abstracts / [s.l. : s. n., 2008]. - p 55
Blutongue Satellite Symposium : Brescia, Italy : 7 June, 2008)
First author Tamba, Marco
Introduction and Objectives Since 2004 the mountain area of the Forlì Province has been interested by a
spontaneous circulation of a vaccine strain of Bluetongue virus serotype 2 (BTVV-2). In this area BTV
modified live vaccine has been never used. The area is 100 kilometres far from the nearest vaccination zone.
Serological and entomological monitoring plans have been yearly carried out in order to follow the evolution of
the problem. Monitoring activities evidenced a relevant virus spread in the area of several mountain
municipalities. At the end of Summer 2004 in this area the prevalence of infection increased from 15,3% to
70,1% in farms and from 2.5% to 20.9% in animals. A survey carried out in the autumn-winter period 20052006 in the same municipalities showed similar prevalences (57.6% in farms and 16.9% in animals): this was
suggestive of a decrease in virus spreading during summer 2005. In this paper the results of an investigation,
made during the autumn-winter period 2006-2007, for the evaluation of the incidence of the transmission of
BTVV-2 strain during summer 2006 are presented; the aim of the survey was to supplement the existing data
on the evolution of the problem. 2.Material and Methods A number of 150 bovine farms located in the studied
area were selected; this sample included all BTVV-2 seropositive farms (96) and a fraction of randomly
selected seronegative farms (54) Sera collected during Bovine Brucellosis surveillance plan were utilised in
order to evaluate the serological status of the selected farms; among this sera only those taken from animals
that were found BTV sero-negative in the previous monitoring were tested again for BTV antibodies. Serum
samples were tested by a cELISA test for BTV antibodies. Positive sera were sent to the National Reference
Centre (CESME, Teramo) for identification of the involved serotype. 3.Results An overall number of 1,287
sera collected in 120 farms were tested. Four animals belonging to 4 different farms resulted positive. Seropositive samples were confirmed as reactors to BTV serotype 2 by sero-neutralization test. 4.Discussion and
Conclusions During 2006 epidemic season the estimated incidence was 0.3% (CI 95%: 0.1%-0.8%). It
appears that the spread of BTVV-2 is continuously decreasing and that , in lack of mass vaccination
campaigns using modified live vaccine, could stop in the next future.
ID nr. 4329
Oliverio° E, Finazzi° G; Daminelli° P, Monastero° P, Bonometti° E, Serraino A, Gregorelli
M, Boni° P
Dynamics of several pathogen microorganisms in packaged water-buffalo mozzarella cheese
Ind Aliment (Pinerolo). - Vol. 49 n 500 ( 2010). - p 28-35. - 6 bib ref
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Pag. 214 di 300 31/08/2011 Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Oliverio, Elisa
The aim of this survey was to evaluate the behaviour of Listeria monocytogenes,
Salmonella typhimurium, Staphylococcus aureus, Escherichia coli O157:H7 in packaged Water-Buffalo
mozzarella cheese. Through two successive trials,the conditioning liquid was contaminated with a mixtures of
these microorganisms. During the first trial contaminated samples were stored at 20°C for 6 days; in the
second samples were stored at four different temperatures for variable times. The results showed that Staph.
aureus is not a risk to the consumer because its concentration reduced at all different storage conditions and
the toxin is never produced. Salmonella typhimurium and E. coli O157:H7 concentration increased only at
20°C but after the end of the shelf-life period established by producers (5 days). In other storage conditions
the concentration of both pathogens didn’t modify or even decreased decreased. L. monocytogenes
decreased at 5°C, while in other storage conditions, after a lag phase variable depending on temperature, the
bacterium showed the capability to grow.
ID nr. 4330
Daffara S, Brazzelli V, Ronzi G, Vicari° N, Fabbi° M, Marone° P
Chronic erythema and fish for salmon in Alaska: an enigma resolved by the laboratory of
microbiology
XXXVIII Congresso Nazionale Associazione Microbiolologi Clinici Italiani (AMCLI) : 17 - 20 Novembre 2009
Rimini / [s.l. : Associazione Microbiolologi Clinici Italiani, 2009]. Congresso Nazionale Associazione Microbiolologi Clinici Italiani (AMCLI) (28 : Rimini : 17 - 20 Novembre
2009)
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
A 50 years-old man, pentathlete and passionate fishing for salmon, after returning from Alaska, in August
2008 came to the clinic of the Division of Dermatology IRCCS S. Matteo di Pavia following the appearance of
erythematous lesions and indolent in his right thigh, right knee and gradually extended to the root of the lower
right rear to the buttock. A clinical appearance histologically characterized mainly by the presence of plasma
cells have suggested to run the anti-Borrelia burgdorferi serology (IFI), which showed a questionable
borderline positivity. Subsequent investigations by ELISA and Western Blot were both positive and led to a
diagnosis of chronic migrant erythema in the course of borreliosis. This diagnosis was further confirmed by
positive PCR for B. burgdorferi sensu lato. Further investigations are underway for the typing of the Borrelia at
genospecie level.
ID nr. 4334
Giovannini° S, Gradassi° M, Zanoni° M, Salogni° C, Alborali° L
Abortion agents diagnostic survey in bovine foetuses in Brescia Province, Lombardy, Italy
EBF 2009 European Buiatrics Forum : 1-3 Dec. 2009 Marseille / editors, R. Maillard and H. Navetat. Marseille : Societé francaise de Buiatrie, 2009. - p 277
European Buiatrics Forum (EBF) (XXV : Marseille : 1-3 Dec. 2009)
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
Pag. 215 di 300 31/08/2011 for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Giovannini, Stefano
The aim of this survey, which ran for a four years period (from 2005 to 2008), was to evaluate the most widely
spread abortive agents in Brescia Province by examining aborted bovine foetuses specimens delivered to the
Veterinary Diagnostic Section in Brescia - Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia
Romagna. The study population consisted of 372 bovine foetuses, which were submitted to necropsy
examination and diagnostic tests; in this survey we did not consider serological data of dairy cows.
Compatibly with the storage conditions and the amount of available material, either bacteriological tests on
samples from liver, brain, lung and abomasal fluid or specific bacteriological tests for the detection of Brucella
spp and Campylobacter spp were performed. Fetal fluids of foetuses over the fifth month of pregnancy were
analyzed with serological tests in order to detect antibodies against Neospora caninum (ELISA), Brucella spp.
(FdC), Leptospira spp. (MAT), BI-IVI (ELISA), BVDV (ELISA), Chlamydophyla psittaci (Cft), Toxoplasma
gondii (IFI and AL) and Coxiella burnetii (ELISA). Specimens of brain tissue were analysed with PCR test for
the detection of Neospora caninum and, in some cases. PCR and cell culture techniques for Chlamydophyla
psittaci and Coxiella burnetii were performed. During the study period it was possible to carry out 324
necropsies, 308 conventional and 296 specific bacteriological tests for Brucella detection, 293 specific culture
for Campylobacter detection, 300 PCR for Neospora detection and different serological tests on foetal fluids to
detect antibodies against Neospora caninum (190), BVDV (236), Toxoplasma gondii (227), Chlamydophyla
psittaci (236), Leptospira spp. (238), BHVI (236), Brucella spp. (227) and Coxiella burnetii (1). Moreover, we
made 44 virological tests and 3 PCR tests for detection of Chlamydophyla psittaci and Coxiella burnetii. Our
findings suggested that the most important cause of abortion in dairy cows in Brescia Province is Neospora
caninum, which showed an overall prevalence of 38% in foetuses brain (PCR) and a seroprevalence of 21%
in fetal fluids. Bacterial agents of septicemic infections were isolateti in 16% of the samples (50 cases): in
particular Streptococcus spp. (40%), Actinomyces pyogenes (28%) and E. coli (16%). Brucella spp. and
Campylobacter spp. tests were negative. Serological results for Chlamydophyla psittaci, Leptospira spp.,
Coxiella burnetii and Brucella spp were negative, whereas, interestingly, a low level of seroprevalence for
BVD (2,9%), Toxoplasma gondii (2,2%) and BHV1 (3,3%) was detected. Virological and PCR tests for
Chlamydophyla psittaci and Coxiella burnetii detection were negative.
ID nr. 4335
Ferris NP, Nordengrahn A, Hutchings GH, Paton DJ, Kristersson T, Brocchi° E, Grazioli°
S, Merza M
Development and laboratory validation of a lateral flow device for the detection of serotype SAT 2
foot-and-mouth disease viruses in clinical samples
J Virol Methods. - Vol. 163 ( 2010). - p. 474-476. - 12 bib ref
Impact factor: 2,133
A lateral flow device (LFD) for the detection of foot-and-mouth disease virus (FMDV) of the SAT 2 serotype
was developed using a monoclonal antibody (Mab 2H6). The performance of the LFD was evaluated in the
laboratory on suspensions of vesicular epithelia: 305 positive for FMDV type SAT 2 from suspected cases of
vesicular disease collected from 30 countries and 1002 samples shown to be negative for FMDV type SAT 2
collected from 67 countries between 1968 and 2008. The diagnostic sensitivity of the LFD for FMDV type SAT
2 was higher at 88% compared to 79% obtained by the reference method of antigen ELISA, and the
diagnostic specificity of the LFD was approximately 99% compared to 100% for the ELISA. The device
recognized FMDV strains of wide diversity within the FMDV SAT 2 serotype and gave a superior performance
for their detection compared to the 1F10 LFD which had been developed previously and shown to perform
less well for the detection of FMDVs of this particular serotype. Reactions in the SAT 2 2H6 LFD with the
Pag. 216 di 300 31/08/2011 viruses of other FMDV serotypes and swine vesicular disease (which produces a clinically indistinguishable
syndrome in pigs), did not occur. These data illustrate the potential for the LFD to be employed to complement
the 1F10 device next to the animal in the pen-side diagnosis of FMD, for providing rapid and objective support
to veterinarians in their clinical judgment of the disease and for specific confirmation of a FMDV type SAT 2
infection.
ID nr. 4336
Cavirani S, Piancastelli C, Ghidini F, Taddei S, Ferrari S, Boldini° M
Immune response by monovalent BoHV-1 marker and BVDV vaccines administered singularly and
associated
Buiatria. - Vol. 4 no 3 ( 2009). - p 3-9. - 5 bib ref
The study is aimed at comparing the humoral and cellular immune response elicided by two vaccines, namely
IBR live-attenuated marker vaccine and BVD inactivated one administered singularly, concurrently or
simultaneously. Results state that either the concurrent or simultaneous administration did not interfere ori the
efficacy of the vaccines. In addition, the simultaneous administration enhanced the immune response of IBR
marker vaccines that could profit by the adiuvant component of the inactivated BVD vaccines. Ori the whole,
the results of the study support the application in field of either the simultaneous or concurrent administration
of IBR live marker and inactivated BVD vaccines that could make simpler the management of the
immunization programs, particularly in large dairy herds.
ID nr. 4337
Mazzoni M, Merialdi° G, Sarli G, Trevisi P, Bosi P
Effect of two doses of different zinc sources (inorganic vs. chelated form) on the epithelial
proliferative activity and the apoptotic index of intestinal mucosa of early-weaned pigs orally
challenged with E. coli K88
Asian Aust J Anim Sci. - Vol. 23 no 6 ( 2010). - p 777-785. - 32 bib ref
We aimed to assess the effect of two doses of different sources, inorganic (zinc oxide) or chelated (zinc
glutamate chelate) on morphology and turn-over of the small intestine of early-weaned pigs orally challenged
with enterotoxigenic E. coli K88 (ETEC). Sixty pigs weaned at 21 days were assigned to one of the following 5
diets: control (C); C+Zinc oxide (ZnO), either a 200 or a 2,500 mg Zn/kg dose; or C+zinc chelate with glutamic
acid (Glu-Zn), either a 200 or a 2,500 mg Zn/kg dose. On d 2, the pigs were orally inoculated with 1.5 ml of a
1010 CFU/ml E. coli K88ac O148 suspension. Zinc supplements did not improve the performance of the pigs,
but on d 5 they reduced the ETEC faecal excretion, and this was mainly due to high zinc doses (p<0.05). The
villous height was improved by the zinc supplements in the duodenal tract (p<0.01) whatever the source and
the level, whereas no effect was seen in the other two tracts. The diet did not affect apoptosis and mitosis
counts, while ETEC-susceptible pigs had more mitotic cells in the villi than non-susceptible pigs, particularly in
the jejunum (p<0.01). The duodenum had fewer mitotic cells in the villi (p<0.05) and in the crypts (p<0.01) and
more apoptotic cells in the villi. High dietary doses of ZnO or Zn-Glutamate improve villous height of the
duodenum, but not of the jejunum and the ileum, and do not affect the epithelial proliferative activity and
apoptotic index of intestinal mucosa of early-weaned pigs orally challenged with ETEC.
ID nr. 4338
Ntafisa V, Fragkiadakia E, Xylouria E, Omirou A, Lavazza° A, Martella V
Rotavirus-associated diarrhoea in foals in Greece
Vet Microbiol. - Vol. 144 ( 2010). - p 461-465. - 33 bib ref
Impact factor: 2,874
Pag. 217 di 300 31/08/2011 Severe outbreaks of diarrhoeic syndrome occurred in young foals at the same stud farm during two
consecutive breeding periods namely spring 2006 and 2007. Rotavirus-like particles were detected by
electron microscopy in the faeces of the affected foals and group A rotavirus infection was confirmed by
Reverse-Transcription (RT)-PCR with selected sets of rotavirus-specific primers. Sequence analysis of the
genes encoding the outer capsid rotavirus proteins VP7 and VP4 enabled classification of the viruses as
G3AP[12] and revealed that the viruses were highly similar to recently reported equine rotavirus strains
circulating in Europe. All Greek equine rotavirus isolates were genetically identical, suggesting persistence of
the same viral strain in the stud farm, over the two consecutive foaling periods.
ID nr. 4345
Catalani E, Amadori° M, Vitali A, Bernabucci U, Nardone A, Lacetera N
The Hsp72 response in peri-parturient dairy cows: relationships with metabolic and immunological
parameters
Cell Stress Chaperones. - Vol. 15 no 6 ( 2010). - p 781-790. - 59 bib ref
Impact factor: 2,167
Project PRF2006201 - Adaptation of farm animals: repercussions on disease occurrence and drug usage Amadori Dr. Massimo - Capofila - MINSAN - 2006 - 861300,00 EUR - 15/01/2008 0.00.00 15/12/2010 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
2 - IZSSA - Nicolussi dr.ssa Paola - E
3 - Università degli Studi della Tuscia (Dipartimento di Produzioni Animali) - Lacetera dr. Nicola - E
4 - IZSME - Martucciello dr.ssa Alessandra - E
5 - IZSUM - Moscati dr.ssa Livia - E
6 - IZSLT - Cavallina dr.ssa Roberta - E
7 - IZSVE - Stefani dr.ssa Anna-Lisa - E
8 - Istituto Superiore di Sanità - Vitale dr. Augusto - E
9 - Università Cattolica del Sacro Cuore - Facoltà di Agraria - Istituto di Zootecnica (Piacenza) Trevisi dr. Erminio - E
10 - UNIVERSITA' MILANO - DIPARTIMENTO MEDICINA VETERINARIA EX ISTITUTO DI
ZOOTECNICA - Verga Dr.ssa Marina - E
The study was aimed at assessing whether the peri-parturient period is associated with changes of
intracellular and plasma inducible heat shock proteins (Hsp) 72 kDa molecular weight in dairy cows, and to
establish possible relationships between Hsp72, metabolic, and immunological parameters subjected to
changes around calving. The study was carried out on 35 healthy peri-parturient Holstein cows. Three, two,
and one week before the expected calving, and 1, 2, 3, 4, and 5 weeks after calving, body conditions score
(BCS) was measured and blood samples were collected to separate plasma and peripheral blood
mononuclear cells (PBMC). Concentrations of Hsp72 in PBMC and plasma increased sharply after calving. In
the post-calving period, BCS and plasma glucose declined, whereas plasma nonesterified fatty acids (NEFA)
and tumor necrosis factor-alpha increased. The proliferative responses of PBMC to lipopolysaccharide (LPS)
declined progressively after calving. The percentage of PBMC expressing CD14 receptors and Toll-like
receptors (TLR)-4 increased and decreased in the early postpartum period, respectively. Correlation analysis
revealed significant positive relationships between Hsp72 and NEFA, and between PBMC proliferation in
response to LPS and the percentage of PBMC expressing TLR-4. Conversely, significant negative
relationships were found between LPS-triggered proliferation of PBMC and both intracellular and plasma
Hsp72. Literature data and changes of metabolic and immunological parameters reported herein authorize a
few interpretative hypotheses and encourage further studies aimed at assessing possible cause and effect
relationships between changes of PBMC and circulating Hsp72, metabolic, and immune parameters in dairy
cows.
ID nr. 4346
Moretto A, Mammone T, Vida P, Lavazza° A, Moretto A
European Content for public health awareness of the Rural population on Avian and Influenza
Pag. 218 di 300 31/08/2011 Pandemic (ECORAIP)
17th International congress of agricultural medicine and rural health : October 13-16, 2009 Cartagena,
Colombia / [s.l : s.n., 2009]. - p. ??
International congress of agricultural medicine and rural health (17th : Cartagena, Colombia : October 1316, 2009)
The population living in rural areas of Europe is crucial to the potential transmission of avian influenza to
humans, due to specific circumstances pertaining to rural life such as backyard poultries, multi-species bird’s
farms, proximity to wetlands where migratory birds stop over, and open market’s custom. This project aims to
provide practical information and guidelines on effectively preventing and managing these potential threats,
targeting the rural population, where a gap of information can be observed. The particular needs of the rural
population and its different characteristics across three European Sub-regions which are representative of the
Southern, Central-Northern and Eastern Europe have been taken into account. Rural life "risk factors" have
been identified in order to develop specific guidelines. Up-to-date scientific knowledge has been evaluated
and criteria defined to integrate or customize the available material of public health campaigns, so that this
can be disseminated in a feasible and effective way to the population. The channels and networks used for
the dissemination of the public health material have been also assessed, reviewing existing efforts aimed at
targeting the rural population. The most effective communication strategies have been identified and specific
guidelines have been developed as the best practice model. The final phase included the practical application
of the guidelines through a pilot test carried out in 30 municipalities of the participating countries (selected
according to their socio-demographic characteristics). The "pilot campaign" has included a presentation and
administration to the local population of a pre/post questionnaire, in order to identify potential deficiencies or
operational problems of the format or content of the information or of the dissemination method. The results of
the questionnaires have been evaluated and used to amend where necessary the text for the final printed
version of the illustrated leaflet. The leaflet is available in five languages (English, Italian, Greek, Polish and
German). Public Health Executive Agency (PHEA) â “ Agreement n. 20067 (2007-2008). Partnership: National
Kapodistrian University of Athens (Greece), Harvard School of Public Health â “ Cyprus (Cyprus), Nofer
Institute of Occupational Medicine (Poland), Techniche Universitat Dresden (Germany).
ID nr. 4346A
Mammone T, Vida P, Lavazza° A, Moretto A
European content for public health awareness of rural population on avian and influenza pandemic
(ECORAIP)
J Rural Med. - Vol. 5 n 1 ( 2010). - p. 95-96
International congress of agricultural medicine and rural health (17th : Cartagena, Colombia : October 1316, 2009)
The population living in rural areas of Europe is crucial to the potential transmission of avian influenza to
humans, due to specific circumstances pertaining to rural life such as backyard poultries, multi-species bird’s
farms, proximity to wetlands where migratory birds stop over, and open market’s custom. This project aims to
provide practical information and guidelines on effectively preventing and managing these potential threats,
targeting the rural population, where a gap of information can be observed. The particular needs of the rural
population and its different characteristics across three European Sub-regions which are representative of the
Southern, Central-Northern and Eastern Europe have been taken into account. Rural life “risk factors” have
been identified in order to develop specific guidelines. Up-to-date scientific knowledge has been evaluated
and criteria defined to integrate or customize the available material of public health campaigns, so that this
can be disseminated in a feasible and effective way to the population. The channels and networks used for
the dissemination of the public health material have been also assessed, reviewing existing efforts aimed at
targeting the rural population. The most effective communication strategies have been identified and specific
guidelines have been developed as the best practice model. The final phase included the practical application
of the guidelines through a pilot test carried out in 30 municipalities of the participating countries (selected
according to their socio-demographic char96 acteristics). The “pilot campaign” has included a presentation
and administration to the local population of a pre/ post questionnaire, in order to identify potential deficiencies
or operational problems of the format or content of the information or of the dissemination method. The results
of the questionnaires have been evaluated and used to amend where necessary the text for the final printed
Pag. 219 di 300 31/08/2011 version of the illustrated leaflet. The leaflet is available in five languages (English, Italian, Greek, Polish and
German). Public Health Executive Agency (PHEA) “ Agreement n. 20067 (2007-2008). Partnership: National
Kapodistrian University of Athens (Greece), Harvard School of Public Health, Cyprus (Cyprus), Nofer Institute
of Occupational Medicine (Poland), Technische Universitaet Dresden (Germany).
ID nr. 4348
Cammi° G, Arrigoni° N, Agnelli° E, Capra D, Belletti° GL
Campylobacter jejuni in the bulk milk for direct sale: dairy herd investigation
Buiatria. - Vol. 4 no 2 ( 2009). - p 11-16. - 21 bib ref
First author Cammi, G
We describe the investigation undertaken in a dairy herd composed of 60 lactating cows, where
Campylobacter jejuni was isolated in bulk milk produced for direct sale using an automatic dispenser. The
microorganism was isolated from one quarter of a cow with a chronie subacute mastitis. We also found C.
jejuni in faeccs from 2 out of 24 (8%) cows and in 1 out of 4 straw bedding sample. When the milk of the
infected cow was excluded from the collection, the contamination of the bulk milk ceased, showing that direct
milk excretion was the source of the C. jejuni rather than faccal contamination during milking process. C. jejuni
mastitis, although rare, must be considered as a possible source of milk contamination. In the described case,
a specific therapy was able to determine both the clinical and bacteriological cure.
ID nr. 4349
Foni° E, Chiapponi° C, Sozzi° E, Barbieri° I, Moreno° AM, Merenda° M, Luppi° A,
Alborali° L, Cordioli° P
Characterization of swine influenza viruses circulating in Italy in 2008-2009
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 159-166. - 11 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Project PRF2006304 - Prevalance of avian and swine influenza virus infection among farmers and animals.
- Vonesch Dr.ssa Nicoletta - UO - MINSAN - 2006 - 300000,00 EUR - 02/01/2008 0.00.00 02/01/2011 0.00.00 - 1 - G2 - Reparto di Batteriologia Specializzata - Cordioli Dr. Paolo - I
First author Foni, Emanuela
Samples from 1854 outbreaks of respiratory disease in Italian pig farms, collected in 2008-2009, were
submitted to RT-PCR for infl uenza virus M gene. From 114 RT-PCR positive samples collected in 2008, 52
swine infl uenza viruses (SIVs) were isolated and subtyped (25 H1N1, 7 H1N2 and 20 H3N2). From 102 RTPCR positive samples collected in 2009, 54 SIVs were isolated and subtyped ( 21 H1N1, 19 H1N2, 13 H3N2
and 1 H1N1 Pandemic 2009). Genetic and phylogenetic analysis of H1N1 strains showed a low degree of
etherogeneity, confi rming the circulation of viruses referring to the “avian–like” Sw/Finistere/2899/82.
Haemoagglutinin (HA) of H3N2 SIVs showed to be strictly correlated to the reference strain A/Port
Chalmers/73, while it was demonstrated that the neuroaminidase (NA) was correlated to NA of recent German
H1N2 SIVs. Phylogenetic analysis of H1N2 SIVs showed that these recent Italian strains were reassortant
strains carrying the HA closely related to European H1N2 SIVs and the NA to the recent H3N2 human
influenza virus.
ID nr. 4350
Sozzi° E, Martinelli° N, Moreno° A, Lelli° D, Fontana° R, Canelli° E, Vinco° LJ, Alborali°
GL, Lombardi° G, Cordioli° P
Pandemic influenza virus (A/H1N1)in pigs
Pag. 220 di 300 31/08/2011 Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 153-158. - 11 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
First author Sozzi, Enrica
Influenza virus A/H1N1, which is currently causing a pandemic, contains gene segments with ancestors in the
North American and Eurasian swine lineages. To get insights into virus replication dynamics, clinical
symptoms and virus transmission in pigs, we infected animals intratracheally with infl uenza pandemic viruses
A/H1N1. In the fi rst trial, nasal discharge, sneezing and fever were observed in two pigs inoculated with
A/It/148/2009 (H1N1).. One pig was sacrifi ced 3 days post-infection (p.i.) and virus detected in lungs, trachea
and nasal swabs. Serum of other infected pig collected 18 days p.i. was positive by NPA-ELISA and HI tests.
In the second trial, pigs inoculated with A/Sw/It/290271/2009 (H1N1) developed only mild fever. Nasal swabs
of all infected animals were positive by rRTPCR 2, 4 and 7 days p.i., and 9 days p.i. in only two pigs. Contact
pigs became infected, shed virus and developed clinical symptoms similar to those shown by the inoculated
animals. Serological response was evidenced by NPA-ELISA and HI starting 9 days p.i. in infected and 14
days p.i. in contact pigs.
ID nr. 4351
Raffi V, Mazzoni C, Rosignoli° C, Bonilauri° P, Spaggiari° B, Gelmetti° D, Gibelli° L, Maioli°
G, Faccini° S, Dottori° M, Luppi° A, Tonon° F
Clostridiosis in the farrowing room: a clinical case report with an atypical onset
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 136-144. - 15 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Project PRC2001006 - Use of PCR for typing C. perfrigens strains isolated from enteric and entero-toxiemic
disease cases of animals and animal source foods. - Rosignoli Dr. Carlo - Solo - MINSAN - 2001 93163464,00 LIT - 03/03/2003 0.00.00 - 02/07/2004 0.00.00 - 1 - M6 - Mantova - Rosignoli Dr.
Carlo - I
In this work the Authors described a syndrome in several suckling piglet broods (one-day old) occurred in a
herd in the Northern Italy and characterised by death 12 hours after the onset of clinical signs. Clinical signs
were characterised by jaundice, lethargy and diarrhea. Dead animals submitted for necropsy to IZSLER,
Reggio Emilia Laboratory, showed good nutritional condition, necrosis of small intestine mucosa and liver
degeneration with foci of parenchymal necrosis. Sow feed micotoxin toxicological investigations were carried
out to elucidate the nature of liver degeneration with negative results. Clostridium perfrigens was isolated from
the small intestine of several piglets (1 x 107 ufc of C. perfrigens from each gram of intestinal content). In
Mantova laboratory (IZSLER) genes for toxins a and ß2 were detected using a multiplex polymerase chain
reaction method from C. perfrigens isolated. Histological examination of samples collected during the
necropsy showed vacuolar liver degeneration, necrotic enteritis and multiple gram-positive, spore-forming
bacterial colonies in the lumen. The syndrome did not appear to be related to dietary or any other specific
management factors. A long acting macrolid administer to piglets in the fi rst twelve hours after the birth let a
complete solution of the syndrome.
ID nr. 4352
Zanoni° MG, Gradassi° M, Moreno° MA, Catella° A, Salogni° C, Sozzi° E, Foni° E,
Cordioli° P, Alborali° L
Pandemic influenza virus (A/H1N1) outbreak in pig farm in North of Italy
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 145-151. - 19 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Pag. 221 di 300 31/08/2011 First author Zanoni, Maria_Grazia
The swine influenza virus (SIV) frequently causes acute respiratory disease in pigs worldwide and is an
important pathogen in the porcine respiratory disease complex (PRDC). The fi rst H1N1 pandemic outbreak in
sow farm in Italy is described in this paper. The main clinical signs were depression and agalactia in sows
during the fi rst week after farrowing. Different weights were observed in litters and piglets showed wasting
and dehydration. The loss interested litters and were quantifi ed in increased mortality in farrowing units,
performance reduction in sows consisting in enhanced mortality and fertility rate reduction.
ID nr. 4353
Candotti° P, Merialdi° G, Guerra° O, Nassuato C, Rota_Nodari° S
Pandemic influenza virus (A/H1N1) outbreak in pig farm in North of Italy
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 121-129. - 28 19 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
First author Zanoni, Maria_Grazia
A survey was performed in eleven pig farms with a post-weaning multisystemic wasting syndrome (PMWS). In
each farm faecal samples were collected from 10 weaner pigs with clinical signs of the disease. A visual
examination of faecal samples was performed on farm and different bacterial counts (total bacterial count,
faecal streptococci, faecal coliformi and Escherichia coli) were determined in laboratory. The effect of orally
administered antibiotics (amoxicillin and/or colistin) on these parameters was studied as well. Compared with
data in literature, coliforms were tendentially higher while total bacterial counts were into ranges
reported.Antibiotics did not signifi cantly affect the bacterial counts examined.
ID nr. 4354
Apicella M, Osella E., Gambino F, Alborali° L, Zanoni° MG, Salogni° C, Bollo E, Guarda F
Sublinical cystitis in young sows: a preliminary study
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 193-197. - 12 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
The authors have examined 594 urinary bladders of young sows aged 9 months and weighing 150-170 kg,
and 69 urine samples for bacterial isolation. In 50 (8,1%) of the urinary bladder, inflammatory foci and ulcers
were detected, in 20 (3,4%) samples catarrhal material, uroliths and sediments, and in 43 (62,3%) samples
several bacterial species were isolated (E. coli, Staphylococcus sp., Streptococcus sp., Serratia sp., Proteus
sp.). The histopathogenesis of the lesions follows the characteristic stages of the inflammatory process, while
the etiological factors responsible for the lesions are not yet fully elucidated. Poor environmental conditions,
stressors and the coitus may be responsible for ascending infections of the urinary tract.
ID nr. 4355
Luppi° A, Bonilauri° P, Ferrari E, Gherpelli° Y, Merialdi° G., Dottori° M
Serological characterization of haemophilus parasuis strains isolated from pathological samples
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 205-211. - 19 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Pag. 222 di 300 31/08/2011 First author Luppi, Andrea
From 2007-2009 a total of 44 Haemophilus parasuis fi eld isolates was collected from diseased pigs in
connection with routine diagnostics of Istituto Zooprofi lattico of Lombardia and Emilia Romagna (IZSLER),
Reggio Emilia Laboratory. The isolates were serotyped by agar gel immunodiffusion test using specifi c
antisera against serovars 2, 4, 5, 12, 13. The choice of antisera used was performed considering the
prevalence of different virulent serotypes described in other european countries. In our study serovar 4 was
the most prevalent (34%) followed by serovar 13 (22,7%) and serovar 5 (15,9%), while 22,7% of the isolates
could not be assigned to a serovar (nontypaple isolates). The strains could be divided into two groups
depending on whether they were isolated from cases with systemic disease (polyserositis, arhritis or
meningitis) or if they only were found in the lower respiratory tract. The most marked difference were
observed for serovar 4, which had a higher prevalence in systemic infection compared to respiratory disease.
The frequency of the isolation of serotypes 5 and 13 from pigs with o without polysierositis were similar, while
nontypaple isolates had a higher prevalence in respiratory disease compared to systemic infection.
ID nr. 4356
Iodice G, Luppi° A, Franchi L, Bonilauri° P, Merenda° M, Dottori° M
Simultaneous infection by serotypes 4 and 12 of Haemophilus parasuis in a swine herd
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 213-220. - 14 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
In a pig-farm of northern Italy, during March-April 2009, was observed an increasing losses in the postweaning period. The piglets showed respiratory symptoms coupled with lameness, nervous signs and pyrexia
(41°C). Two serovars of Haemophilus parasuis were isolated from diseased pigs in connection with routine
diagnostics of Istituto Zooprofi lattico of Lombardia and Emilia Romagna (IZSLER), Reggio Emilia Laboratory.
The strains isolated were serotyped using the agar gel immunodiffusion test and indirect haemoagglutination.
Two strains isolated from pigs with systemic disease (polysierositis) belonged to serovar 4, while another
strain isolated from pig with bronchopneumonia was serotyped as serovar 12. This results agreed with the
classifi cation of Kielstein and Rapp-Gabrielson (1992), in which the serovar 12 has been shown to be highly
virulent causing death in SPF pigs within 4 days after infection without polysierositis. In contrast, in the same
classifi cation, serovar 4 would be moderately virulent causing polysierositis and generally not death.
However, the connection between serovar isolated and gross lesions observed can be influenced by several
factors (host factors, vaccinations and other bacterial and viral diseases). In this work H. parasuis coexisted
with other pathogens like Actinobacillus pleuropneumoniae and PRRSV. The results of this work showed the
importance of isolates serotyping, mainly if the vaccination is to be used for disease protection.
ID nr. 4357
Gradassi° M, Pavesi° R, Boniotti° B, Nassuato° C, Giovannini° S, Giacomini E, Bellini° S,
Pacciarini° M, Alborali° L
Detection of PRRSVin oral fluid samples: longitudinal study under field conditions
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 225-231. - 12 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
PRRS (Porcine Reproductive and Respiratory Syndrome) control in swine farms is an ambitious challenge
that has to be based on different measures and intervention plans and that, because of the high amount of
diagnostic samples required, is often cost prohibitive. The selection of oral fl uid as diagnostic specimen is
likely to be a promising alternative to serum. The main purpose of the present experimental study was to
monitor PRRSV infection towards a longitudinal sampling of sera, according to the traditional diagnostic
protocols, and to match sera with collective pen-based oral fl uid results. The preliminary data presented here
Pag. 223 di 300 31/08/2011 suggests that the diagnostic sensitivity of the quantitative RT-PCR on oral fl uids, compared to the gold
standard (RT-PCR on individual serum samples), can be accounted as satisfactory.
ID nr. 4358
Dotti° S, Sandri GP, Villa° R, Razzuoli° E, Sossi° E, Amadori° M
Kinetic development of immune response to Porcine Reproductive and Respiratory Syndrome
(PRRS) in gilts
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 232-237. - 7 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Project PRRSV AUTOFINANZIATO - Evaluation of the immune response to Porcine Respiratory and
Reproductive syndrome virus (PRRSV) - Ferrari Dr.ssa Maura - Solo - IZSLER - 2009 - 75000,00
EUR - 31/07/2009 0.00.00 - 31/12/2010 0.00.00 - 1 - K4 - REPARTO SUBSTRATI CELLULARI E
IMMUNOLOGIA CELLULARE - Ferrari Dr.ssa Maura - I
2 - M1 - Sezione diagnostica provinciale di Brescia - Alborali Dr. Loris - I
First author Dotti, Silvia
The aim of this study was to evaluate the time-course of the immune response to PRRS virus in two PRRSVpositive farms, hereunder named SG and M. Gilts originating from a PRRS-negative multiplier had to be
introduced into two PRRS positive commercial herd and the purpose was to house them without creating
PRRS outbreaks in the receiving farms under study. For this purpose, two different strategies were adopted:
vaccination in farm SG and direct contact with viremic animals in farm M. The cell-mediated immune response
was evaluated by a PRRSV-specifi c interferon- release assay; the results showed, in both farms, that the
cell-mediated response occurred at a low level and frequency, as opposed to the Ab response. This fi nding
confi rms the results by other authors, i.e. the discrepancy between the two kinds of immune response to
PRRSV (humoral and cell-mediated), following both vaccination and direct contact with viremic animals.
Moreover, with saliva samples collected in farm M, an ELISA assay was performed to detect PRRSV-specifi c
IgA antibodies, the assessment of which could be useful in association with other routine diagnostic methods.
ID nr. 4359
Bresaola M, Lombardo T, Villa° R, Sossi° E, Ferrari° M
Retrospective study on Swine Torque Teno virus genogroups 1 and 2 and Porcine circovirus type 2
coinfection in italian pig sera (from 1990 to 2009)
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 239-247. - 32 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Torque teno virus (TTV) was fi rst isolated from a human hepatitis patient in 1997. TTV was also identifi ed in
several animals, including pigs, cattle, sheep, cats and dogs. Recent studies suggest that swine TTV (swTTV)
could play aetiological roles in pig diseases, in particular in post-weaning multisystemic wasting syndrome. In
this retrospective study, we analysed the prevalence of swTTV, genogroups 1 (swTTV1) and 2 (swTTV2), and
Porcine Circovirus type 2 (PCV2) in Italian pig sera between years 1990 and 2009. The main objective is to
assess whether there was a statistically signifi cant association between swTTV and PCV2 infection. Taking
into account the whole study period, 73 out of 95 animals (76,84%) were infected with one or the other
genogroup of swTTV, while 27 out of 95 pigs (28,42%) were co-infected with both genogroups. swTTV
genogroup 1 (54 out of 95, 56,84%) was more prevalent than genogroup 2 (46 out of 95, 48,42%). Moreover,
41 out of 95 animals (43,16%) were infected with PCV2. Then, 20 out of 95 animals (21,05%) were coinfected with swTTV1 and PCV2, while 27 out of 95 animals (28,42%) were co-infected with swTTV2 and
PCV2. These results suggest that there is a statistically signifi cant association between swTTV2 and PCV2
infection.
Pag. 224 di 300 31/08/2011 ID nr. 4360
Candotti° P, Nassuato° C, Rota_Nodari° S
Higher serum lypopolysaccarides in weaned piglets affected by syndromes of wasting
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 248-253. - 12 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
First author Candotti, Paolo
Five healthy farms and 13 farms with a post-weaning multisystemic wasting syndrome were selected for the
study. Ten weaners were blood sampled in each farm. Blood samples were tested for lypopolisaccarides
(LPS), Il-10 and TNF-a level. Results showed that the animals with a wasting syndrome had a higher serum
level of LPS compared with healthy animals. The higher level of LPS could be associated with the wasting
syndrome observed. It was individuated a cut-off of 0,44 EU/ml (sensitivity of 93% and specifi city of 82%). Il10 and TNF-a were not associated with LPS levels.
ID nr. 4361
Sarli G, Morandi F, Panarese S, Bacci B, Ferrara D, Fusaro L, Bacci ML, Govoni N,
Dottori° M, Bonilauri° P, Lelli° D, Leotti G, Vila T, Josel F, Ostanello F
Conventional sows inseminated with artificially PCV2 infected semen
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 254-263. - 23 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
Six conventional sows were inseminated with PCV2 added semen (infected) and three animals with semen
and viral medium (controls). At ultrasonography (day 29 post insemination), only three out of the six infected
animals were pregnant, unlike controls, all pregnant. Viremia was demonstrated in 4 out of 6 infected animals,
along with a mean antibody titre higher only in exposed sows. Among infected, the sow with the lowest anti
PCV2 titre (1/100) at the beginning of the experiment, kept displaying positive blood results over time and had
also the highest number of PCV2 positive foetuses (10/16). One placenta displayed mild focal necrosis of the
chorionic epithelium, positively stained by IHC for PCV2 antigen.The results obtained suggest that: 1)
conventional sows can be infected by intrauterine exposition; 2) low antibody titres increase the probability of
infection; 3) PCV2 infection close to insemination time reduces the pregnancy rate; 4) placental lesions may
represent an additional cause of fetal suffering.
ID nr. 4362
Canelli° E , Catella° A, Alborali° l, Sozzi° E, Lelli° D, Moreno° A, Fontana° R, Cordioli° P
Preliminary study on the genetic variability of PCV2 strains isolated in the 1999-2002 period and
since 2007 up to now
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 265-270. - 18 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
First author Canelli, Elena
Porcine circovirus 2 (PCV2) is associated with several diseases in pigs, all defined as PCVD (porcine
circovirus associated diseases), that are now considered as a global problem causing significant economic
losses in the swine industry. The virus has been classifi ed by phylogenetic analysis into two genetic types of
PCV2a (defi ned also as cluster 2) and PCV2b (cluster 1). Although the PCV2b was commonly associated
Pag. 225 di 300 31/08/2011 with PMWS, there are only hypotheses about the differences in viral virulence between the two subtypes. In
order to differentiate the two PCV2 subtypes, two polymerase chain reaction (PCR) assays were developed.
In this study, 30 PCV2b strains, 2 PCV2a strains and 4 strains showing both genotypes on a total of 36 strains
were detected. All the PCV2 strains were originated from farms with PMWSanamnesis or from swine with
pathological lesions. The results showed that the PCV2b is the prevalent genotype in both period in the
analyzed area.
ID nr. 4363
Magistrali CF, Cucco L, D’avino N, D’angelo G, Gherpelli° Y, Bonilauri° P, Merialdi° G
Evaluation of antimicrobial susceptibility of B. hyodysenteriae: comparison between two methods
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 337-340. - 8 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
The aim of this study was to compare two different methods available for the evaluation of antimicrobial
susceptibility of Brachyspira hyodysenteriae. Two different tests used for determination of minimal inhibitory
concentration (MIC) were compared: the Quick- MIC agar dilution method and the Broth dilution method. 20
B. hyodysenteriae strains, isolated from pigs affected by Swine Dysentery of Italian swine herds were tested
blindly and independently by two different diagnostic laboratories. Results confi rmed what already described
in literature: the Broth dilution method give lower MICs compared to the agar dilution method (Quick MIC).
According to the MIC breakpoints thresholds (Ronne & Szancer, 1990), some strains classifi ed as
intermediate by the agar dilution method (Quick MIC), resulted sensitive by the Broth Dilution method.
Nevertheless, no isolate was classified resistant by agar dilution method (Quick MIC) resulted sensitive by the
Broth Dilution Method or vice versa. In conclusion, classifi cation of B. hyodysenteriae isolates in different
susceptibility groups using the two tests can occur, in particular for those showing intermediate values.
ID nr. 4364
Mazzoni C, Tonon F, Borri E, Raffi V, Scollo A, Bonilauri° P
Analysis of sow returns: preliminary studies
Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 347-355. - 10 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
The maintenance and persistence in sow pregnancy, go through the production by
the embryo of two different hormonal signals. Failure to produce these signals may allow us to understand the
different classes of returns in estrus that, in sows can be divided into three category: Regular Returns of fi rst
type (between 18 and 23 days), Irregular Returns (between 24 and 38 days) and Regular Returns of second
type (between 39 and 44 days). The present paper analyse the reproductive data of 2008 and 2009 of two Po
Valley farms, by comparing together the various classes of return. Particular attention is given to Irregular
Returns, divided in fi rst and second type, according to the transition from embryonic to fetal stage. Results
showed a less stability during the embryonic stage of pregnancy related to fetal stage. The importance of
studying and understanding these mechanisms could help in finding effective solutions to contain the
embryonic mortality in a very sensitive stage of gestation, especially since due to the application of the law for
pigs protection, running the necessity to herd together sows, gilts at the fourth week of gestation.
ID nr. 4365
Arioli E, Caleffi A, Luppi° A, Bonilauri° P, Maioli° G, Dottori° M, Marco E
Actinobacillus pleuropneumoniae eradication program in a pig herd
Pag. 226 di 300 31/08/2011 Atti Convegno SIPAS. - Vol. 36 ( 2010). - p 402-413. - 2 bib ref
Meeting Annuale della Societa' Italiana di Patologia ed Allevamento dei Suini (SIPAS) (36 : Montichiari (BS)
: 25-26 Marzo 2010)
The Authors describe the Actinobacillus pleuropneumoniae (APP) eradication program application results in a
pig Italian herd in which APP disease had a very important economic impact reducing pig production. From
2005 to 2008 APP biovar 1 serotype 9 was isolated repeatedly from the herd and a very high seroprevalence
for APP was observed. The eradication program used partial depopulation of sow and a complete
depopulation of animal under 10 months of age, coupled with treatment with enrofl oxacin and fl uorfenicol in
sow and tulatromycin in piglets. The antimicrobials were selected on the basis of MIC (minimal inhibitory
concentration) results of APP strain isolated in the herd. The level of bio-security was considered before
eradication and implemented during the program application, in order to reduce the risk of APP reinfection in
the herd. Twelve months after the starting of the eradication program, the clinical and laboratory findings
suggested that the APP eradication had succeeded in this herd. The comparative application and results of
slaughterhouse pleurisy evaluation system (SPES) on lungs belonging to batches of pigs before and after the
application of the eradication program showed a high and absent incidence of pleural lesions associated to
APP infection (grade 2, 3 and 4) respectively. Production data showed an improvement after the eradication
program application.
ID nr. 4366
Merenzoni ML, Passamonti F, Coppola G, Maranesi M, Capelli K, Cappomazzio S, VeriniSupplizi A, Cordioli° P, Coletti M
Gammaherpesvirus infections in foals
International congress XVI SIVE : 29th-31st, January 2010 Marina di Carrara : proceedings / [s.l. : s.n.,
2010]. - p 245-246. - 4 ref bib
International congress Società Italiana Veterinari per Equini (SIVE) (16 : Marina di Carrara : 29th-31st,
January 2010)
ID nr. 4367
Daminelli° P, Finazzi° G, Oliverio° E, Romagnoli L, Gobbini° S, Boni° P
Shelf-life assessment of Modena DOP raw ham sold in vacuum packed pieces
Ind Aliment (Pinerolo). - Vol. 49 no 501 ( 2010). - p 36-41. - 4 bib ref
Project PRC2007001 - Determination of quality and safety features based on predictive microbiology and
risk assessment of traditional regional food products - Finazzi Dr. Guido CON DELIBERA DG N. 461
DEL 17/09/2010 SOSTITUITO DR. BONI CON DR. FINAZZI - Solo - MINSAN - 2007 - 615000,00
EUR - 01/09/2008 0.00.00 - 31/08/2010 0.00.00 - 1 - I3 - Reparto Microbiologia e Parassitologia
degli Alimenti e Sorveglianza Epidemiologica - Boni Dr. Paolo - I
2 - M6 - Sezione diagnostica provinciale di Mantova - Franzini Giuliana - I
3 - M9 - Sezione diagnostica provinciale di Sondrio - Bertoletti Dr.ssa Irene - I
4 - N1 - Sezione diagnostica provinciale di Bologna - Bardasi Dr.ssa Lia - I
5 - N6 - Sezione diagnostica provinciale di Piacenza - Cammi Dr.ssa Giuliana - I
6 - N7 - Sezione diagnostica provinciale di Ravenna (Lugo di Romagna) - Raffini Dr. ssa
Elisabetta - I
First author Daminelli, Paolo
The aim of this survey was to evaluate the behaviour of Listeria monocytogenes during shelf-life of vacuum
packed Modena DOP raw ham cut in pieces. For this purpose different pieces of cured ham have been
externally contaminated with this microorganism, vacuum packed and stored at four different temperatures:
5°, 10°, 15°, 20°C. The results show that Listeria monocytogenes is not a risk for the consumer because its
concentration decreases, during a shelflife period of 6 months, at all different storage conditions.
Pag. 227 di 300 31/08/2011 ID nr. 4374
Moreno° A, Di_Trani L, Alborali° L, Vaccari G, Barbieri° I, Falcone E, Sozzi° E, Puzelli S,
Ferri G, Cordioli° P
First pandemic H1N1 outbreak from a pig farm in Italy
Open Virol J. - Vol. 4 ( 2010). - p 57-61. - 17 bib ref
Project PRF2006301 - Diagnosis and prophylaxis of avian influenza and study of the viral evolution
processes and interspecies transmission - Capua Dr.ssa Ilaria - UO - MINSAN - 2006 2147000,00 EUR - 01/12/2007 0.00.00 - 30/11/2010 0.00.00 - 1 - G1 - Reparto di Virologia e
Sierologia Specializzata - Moreno Martin Dr.ssa Ana - I
PRF2006304 - Prevalance of avian and swine influenza virus infection among farmers and animals.
- Vonesch Dr.ssa Nicoletta - UO - MINSAN - 2006 - 02/01/2008 0.00.00 - 02/01/2011 0.00.00 300000,00 EUR 1 - G2 - Reparto di Batteriologia Specializzata - Cordioli Dr. Paolo - I
First author Moreno_Martin, Ana
The lirst outbreak of the pandemie IIINI virus in a swine breeder farm in Italy in November 2009 was reported.
Clinical signs observed in sows included fever, depression, anorexia and agalactia, while in piglets diarrhoea
and sveight loss. The morbidity in sows was approximately 30% and the accumulateti mortality rate was
similar with those usually reported in piggeries «10%). Virus was isolated from piglets (A/Sw/lt/290271/09) and
the sequencing of the whole genome was then performed. Comparison with ali (H1N1)v sequences available
in GenBank shows A/Sw/lt/290271/09 three unique amino-acid (aa) changes in P132 (S405T), PBl (K386R)
and PA (K256Q), not yet associated to any well characterized phenotype markers of Influenza viruses. Ali
eight aa at positions representing the socalied species specific swine-human signatures, found in both swine
and in the pandemic H1Nly, are also present. The M2 protein displays the C55F and the PA protein the
S409N substitutions, both corresponding to enhanced transmission phenotype markers. Phylogenetic analysis
showed that the virus was genetically related to the pandemic HIN 1 virus. In addition, serological samples
were collected from 40 sows, of which 20 resulted positive to the pandemic H1N1 virus by HI test proving a
virus circulation in the Farm.
ID nr. 4375
Maresca C, Bartoccioni S, Bellini° S, Costarelli S, De Curtis M, Faccenda L, Ferrarini N,
Flamini AR, Pauselli GB, Scoccia E, Scorcelletti S, Cenci T
Swine vesicular disease, updates to December 31, 2008
Sanità Pubblica Veterinaria http://spvet.it/arretrati/numero-56/webzine.html - ultimo accesso 26/05/2010. Vol. 56 ( 2009). - p 10-25
Swine vesicular disease is a disease caused by a virus of the family Picornaviridae genus Enterovirus, is
characterized by high diffusibility and therefore by a strong economic and social impact. In Italy, over the past
5 years, there have been numerous outbreaks; in Umbria outbreak occurred in late 2008, involving 30
outbreaks in the Province of Perugia.
ID nr. 4376
Gale P, Estrada-Pen A, Martinez M, Ulrich RG, Wilson A, Capelli G, Phipps P,
De_La_Torre A, Munoz MJ, Dottori° M, Mioulet V, Fooks AR
The feasibility of developing a risk assessment for the impact of climate change on the emergence of
Crimean-Congo haemorrhagic fever in livestock in Europe: a Review
J Appl Microbiol. - Vol. 108 ( 2010). - p 1859-1870. - 60 bib ref
Impact factor: 2,098
Project EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Pag. 228 di 300 31/08/2011 Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
Crimean-Congo haemorrhagic fever virus (CCHFV) is one of the most widespread of all medically important
arboviruses with ticks of the Hyalomma spp. serving as the main vectors. Infection of livestock by CCHFV
serves as a route of exposure to humans, as a reservoir of disease and as a route of importation. This study
discusses the pathways and data requirements for a qualitative risk assessment for the emergence of CCHFV
in livestock in Europe. A risk map approach is proposed based on layers that include the potential routes of
release (e.g. by migrating birds carrying infected ticks) together with the main components for exposure,
namely the distributions of the tick vectors, the small vertebrate host reservoirs and the livestock. A layer on
landscape fragmentation serves as a surrogate for proximity of livestock to the tick cycle. Although the impact
of climate change on the emergence of CCHF is not clear, comparing the distribution of risk factors in each
layer currently with those predicted in the 2080s with climate change can be used to speculate how potential
high-risk areas may shift. According to the risk pathway, transstadial and / or transovarial transmission in the
tick vector are crucial for CCHFV spread. Vector competence and tick vector switching, however, remain
critical factors for CCHFV colonization of new regions in Europe. The species of migratory bird is also an
important consideration in the release assessment with greater abundance and biodiversity of ground-dwelling
birds in southern Europe than in northern Europe.
ID nr. 4377
Calzolari° M, Bellini R, Medici A, Bonilauri° P, Sambri V, Cavrini F, Dottori° M, Cordioli° P,
Angelini P
Testing the vertical transmission of chikungunya virus in Aedes albopictus using the actors of the
2007 outbreak in Italy
Trop Med Int Health. - Vol. 14 suppl 2 ( 2009). - p 49
European Congress on Tropical Medicine and International Health : 6th Mediterranean Conference on
Migration and Travel Health : 1st : Verona, Italy : 6-10 September 2009)
Impact factor: 2,312
First author Calzolari, Mattia
Experiments were conducted to determine whether transovarial transmission (TOT) of chikungunya virus
(CHIKV) occurs in Aedes albopictus, the species responsible of the 2007 CHIK outbreak in Emilia-Romagna
region (north-eastern Italy) – the first outbreak of this disease in a temperate country. Ae. albopictus eggs
collected in the epidemic area were reared under standard laboratory conditions and F1 females were used
for the study. In BL3 laboratory, females were orally exposed through a membrane to different concentrations
of virus (from 104.5 to 107.3 TCID50/ml) in human washed eritrocites. Two experiments were performed
using a strain of CHIKV isolated from mosquitoes collected in the epidemic area and a strain from a viremic
patient coming from Cesena. After the first exposition to the viruses, the females were blood fed with two
more non infected blood meals in order to obtain two more successive ovipositions. To verify the presence
and the diffusion of the virus in the mosquitoes, females were sampled at different time after infection and
body and legs were separately tested by Real Time PCR (RTPCR). The eggs obtained from the three
gonotrophic cycles were reared in laboratory and the adults obtained were individually tested by CHIKV RTPCR. A total of 101 females took the infected blood meals. All the females fed with the two highest
concentrations of CHIKV human strain showed virus diffusion, as proved by positive PCR on the mosquito
legs at the end of experiment. Whereas only the females fed with the highest concentration of the CHIKV
mosquito strain showed the virus diffusion, proved as above. A total progeny of 1056 adults were tested by
RT-PCR for the presence of virus and three positive specimens were found in the progeny – 2 males and 1
female – all stemming from the second gonotrophic cycle. Based on these studies, infected females were
capable of transmitting the virus vertically to their offspring at a low rate. If the TOT observed in our study
represents the natural maximum rate of vertical female capability of transmitting the virus to their progeny,
then the observed TOT rate was probably not sufficient to guarantee the maintenance of the virus in a
temperate region during the winter period. This supposition could be supported by the observation of no
autochthonous cases of CHIK fever in the same area during summer 2008.
Pag. 229 di 300 31/08/2011 ID nr. 4378
Rubini° MN, Losio° MN, Pavoni° E, Bianchi° L, Galletti° G, Scordella G, Bresolin R,
Pongolini° S, Piano A, Serratone P
Occurrence of Vibrio spp. in shellfish harvested from Emilia Romagna coast (North-western Adriatic
Sea, Italy)
7th International Conference on Molluscan Shellfish Safety : Nantes, France 14-19 June 2009 : conference
handbook / [s.n. : s.l., 2009]. - p 193
International Conference on Molluscan Shellfish Safety (7th : Nantes, France : 14-19 June 2009)
Project PRF2006303 - Food quality and safety of animal products, especially related to seafood, seafood
process and distribution chain - Cancellotti dr. Maria Francesco - UO - MINSAN - 2006 1248000,00 EUR - 15/12/2007 0.00.00 - 14/12/2010 0.00.00 - 1 - I3 - Reparto Microbiologia e
Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Losio Dr.ssa Marina Nadia - I
First author Rubini, Silva
This work aimed at determining the contamination of shellfish harvested in Northern Italy with Vibrio spp.. This
study has been carried out in the province of Ferrara since 2006. The monitoring area is situated in the Northwestern Adriatic Sea, from the mouth of Po River to the mouth of Reno River. A total of 117 samples,
coliected in 31 stations, were analysed. In addition physico-chemical parameters of the harvesting water were
also measured (salinity and water temperature). Vibrio spp. was isolated in 85,5% of the samples. Only 17
samples (14,5%) resulted negative for Vibrio spp.. V. alginolyticus was the most frequently isolated (70,9%),
followed by V. parahaemolyticus (38,5%) and V. cholerae (10,3%). Many samples (32,5%) contained two or
more species of Vibrio spp. The analysis included the compulsory microbiological parameters Escherichia coli
and Salmonella spp., according to European legislation (Reg. EU 853/2004, 85412004 and 144112007), as
well as the determination of the contamination with Vibrio spp.. The isolates were characterized by classical
biochemical tests, API 20E (bioMérieux), and PCR technique based on specie-specific and pathogenic genes:
toxR, tl, tdh, trh for V. parahaemolyticus, toxR, hlyA, ctxA, tcpl for V. cholerae and vvha and viuB for V.
vulnificus. One V. parahaemolyticus strain was trh+, and two were tdh+. Multiplex PCR was also employed to
research V. cholerae-virulence associated factors as cholera toxin (ctxA gene), hemolysin (hlyA gene), non 01
heat-stable enterotoxin (stn/sto gene) and outer membrane protein (tcpA gene). Although almost ali the
analysed molluscs complied with the UE legislation, potential human pathogens were detected in a wide
range of samples. These results evidence that the presence of Vibrio spp. in shellfish is common and confirm
the need for a specific shellfish monitoring pian to identify the presence and the effective role of potential
pathogen Vibrio species to protect public health.
ID nr. 4379
Croci L, Losio° MN, Arcangeli G, Pepe T, Pavoni° E, Magnabosco C, Ventrone J,
Suffredini E
Survey of enteric viruses presence in seafood products in Italy (1999-2008)
7th International Conference on Molluscan Shellfish Safety : Nantes, France 14-19 June 2009 : Conference
handbook / [s.n. : s.l., 2009]. - p 51
International Conference on Molluscan Shellfish Safety (7th : Nantes, France : 14-19 June 2009)
Project PRF2006303 - Food quality and safety of animal products, especially related to seafood, seafood
process and distribution chain - Cancellotti dr. Maria Francesco - UO - MINSAN - 2006 1248000,00 EUR - 15/12/2007 0.00.00 - 14/12/2010 0.00.00 - 1 - I3 - Reparto Microbiologia e
Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Losio Dr.ssa Marina Nadia - I
First author Rubini, Silva
Enteric virus contamination in shellfish has been increasingly recognised as a risk for public health. In Italy, in
the last decade, the surveiliance of enteric virus in seafood products has progressively joined the official
controls required by the European legislation. From 1999, after the first pilot study on Hepatitis A (HAV) in
Pag. 230 di 300 31/08/2011 shellfish at market retail in Puglia, the screening has enlarged to Norovirus (NoV), Enterovirus (EV, year 2001)
and Rotavirus (RV, 2003). In the period 1999-2008, with progressive improvements in the detection and
characterization methods (conventional PCR, real-time PCR, sequencing, etc.), were analysed between 1200
(RV) and 2500 (HAV) samples, depending on the virus considered. The monitoring included shellfish at
consumption level and at production stage (sampling from class A and B harvesting areas in Adriatic and
Tyrrhenian sea), national and imported products, products suspected for epidemics, as well as prepared and
treated seafood products. Overall, at retail level enteric virus contamination involved approximately 5% of the
samples (5.8% with HAV, 5.2% EV, 2.9% NoV and 0.9% RV), while higher contamination was detected in
samples from ciass B harvesting areas. Genotypes most frequently detected were 1A and 113 for HAV and
GlIA, Glib, G11.1 and G11.2 for NoV. For cuitivable viruses, presente of infective virus was confirmed in a low
percentage of the positive samples (5.4% for HAV and 13.2% for EV). These data provide a preliminary
picture on the circulation level of different enteric viruses in seafood products. Further systematic studies on
harvesting areas, carried on with the use of standardized methods and taking into account the environmental
conditions influencing virus survival and spread, are needed to evaluate the safety problem of virai
contamination in shellfish. This work was partially supported by the Integrated Research Project
SEAFOODpIus (Sixth Framework Programme - contract N° FOOD-CT-2004-506359).
ID nr. 4380
Galmozzi G, Muraro M, Vandoni S, Bonfanti M, Faccini° S, Rosignoli° C, Sgoifo_Rossi CA
Treatment regimes of respiratory disease in newly received feedlot cattle
Large Anim Rev. - Vol. 15 no 6 ( 2009). - p 257-266.- 29 ref bib
Four trials were carried out to investigate the efficiency of gamithromycin in beef cattle's bovine respiratory
disease (BRD) prevention and therapy. The gamithromycin is characterized by fast absorption and distribution
to target tissue, notably the lung. Three studies were conducted on the preventive efficacy using an untreated
control (trial 1), a long-acting oxytetracycline formulation (trial 2) and tulathromycin (trial 3). The responses of
tulathromycin and Gamithromycin were compared in the therapeutic study (trial 4). Evaluations included
incidence of morbidity, mortality, re-treatments and problem animals (removed to hospital pen) over the 14
days subsequent to treatment and shortterm growth rates over the first 30 days. Preventive treatment with
Gamithromycin significantly reduced the morbidity due to BRD by 86%, 86% and 35% compared to the control
groups in trials 1, 2 and 3 respectively. The proportion of problem animals was significantly less in the
Gamithromycin group compared to the controls in trial 2. In the therapeutic trial, the incidence of animal that
required re-treatment during the 14 days following treatment was 30,8% in the Gamithromycin group, in which
there were no problem animals, compared to 81,8% in the positive control group, in which problem animals
incidence was 27,7% (significative differences). Growth rates were significantly less in the control animals in
trial 1. Overall, these results confirm the usefulness of Gamithromycin in BRD treatment and prevention and
provide valuable information about BRD economic balance\.
ID nr. 4381
Bonardi S, Paris A, Salmi F, Bacci C, Floriani ME, Bignami S, D'Incau° M, Tagliabue° S,
Brindani F
The role of pigs as pharyngeal carriers of human pathogenic Yersinia enterocolitica strains
AIVI Rivista dell’Associazione Italiana Veterinari Igienisti. - Vol. 3 ( 2009). - p 51-54. - 23 bib ref
From March 2007 to January 2008, a total of 170 pigs at slaughter were tested for Y. enterocolitica
contamination in tonsils tissue. The animals carne from 125 different farms located in four regions of Northern
Italy Y. enterocolitica was isolated from 19 out of 170 (11.2%) tonsils samples. The prevalent bio-serotype
(68.4%) was 4/0:3, followed by bioserotypes 1A/0:8 (15.8%), IA/0:5 (10.5%) and 4/0:8 (5.2%). Among bioserotype 4/0:3, several strains possessed yadA, ail and ystA virulente genes.
Pag. 231 di 300 31/08/2011 ID nr. 4382
Bellini R, Bonilauri° P, Angelini P, Albieri A, Veronesi R, Calzolari° M, Dottori° M, Tamba°
M, Venturi L, Venturelli C, Borrini B, Martini E
Evidence of persistant activity of West Nile Virus in the Po plain area of Italy
5th International Congress of Vector Ecology : 11-16 October 2009 Delek, Antalya, Turkey : proceedings /
[s.l : s.n., 2009]. - p 194
International Congress of Vector Ecology (5. : Delek, Antalya, Turkey : 11-16 October 2009)
During the late summer 2008 a large epidemic of West Nile Virus occurred in North-East Italy involving an
area of more than 7,000 km' in 3 Regions (Lombardia, Veneto, Emilia-Romagna). This was the largest
epidemie ever recorded in the country. Following the first evidence in equine and birds an active
entomological surveillance plan was started by the Emilia-Romagna Surveillance Group on Vectorial Disease.
Mosquito collection was conducted by C02 baited traps specifically positioned in 78 sites. Totally in the period
September 3 - October 23. more than 20.000 mosquitoes of which 53% Culex pipiens. 43% Aedes easpius.
2% Aedes albopictus, 1% Aedes vexans, 0.2% Anopheles maculipennis, 0.02% Culex modest us, 0.02%
Culiseta annulata were analyzed by RT-PCR. Two pools of Cx.pipiens collected at the end of September in
different localities teak teed positive for WN virus. In the 2009 summer the surveillance was activated focusing
on sentinel equine, residential birds (corvidis) and mosquitoes. Mosquito collection was conducted in the
period week 25-42 by CO2 traps in 75 fixed stations with weekly to monthly periodicity. Totally more than
150,000 mosquitoes were collected and analyzed. Starting from the end of July WN positivity was detected in
Culex pipiens pools, magpie (Pica pica), and equines in and around the WN interested area 2008. No human
cases were registered at the time of abstract submission (August 31). The persistence of WN activity in the Po
plain for two consecutive years indicates that the area is becoming suitable for WN establishment and
possible endemicity, stressing the need to organize standard surveillance measures aimed to the early
detection of WN activity and risk evaluation in public health.
ID nr. 4384
Circella E, Pennelli D, Tagliabue° S, Ceruti R, Giovanardi D, Camarda A
Virulence - associated genes in Avian Pathogenic Escherichia coli of turkey
Ital J Anim Sci. - Vol. 8 no 4 ( 2009). - p 775-779. - 7 bib ref
Impact factor: 0,132
50 Escherichia coli (APEC-Avian Pathogenic Escherichia coli) strains and 15 E. coli (AFEC-Avian Faecal
Escherichia coli) from turkeys affected by colibacillosis and from healthy turkeys were tested for the presence
of eight different virulence-associated genes. Besides, APEC were serotyped. O78 has been the most
detected serotyped. The presence of the tested virulence genes was prevalently related to the APEC isolates.
With reference to serogroup, all the tested O78 resulted iss and irp2 positive. Besides, the e cva/cvi were
respectively present in 88.9 and 83.3% of O78. Nevertheless, the finding of a not type able strains equipped
with all the eight tested virulence genes among the APEC isolates suggest the importance of a careful and
complete characterisation of the isolate to evaluate the real potential pathogenic attitude of the bacterium.
ID nr. 4390
Ferrari° M, Renzi° S, Dessì S, Diaferia G, De_Blasio P, Biunno I
New cryosolutions and cryopreservation strategies used in a Mesenchymal Stem Cells (MSCs) bank
Diversity in biobanking: embracing differences, harnessing commonalities : ISBER 2010 Annual Meeting &
Exhibits : May 11-14, 2010 Rotterdam, Netherlands / [s.l. : s.n., 2010]. - p 45
ISBER 2010 Annual Meeting & Exhibits : Rotterdam, Netherlands : May 11-14, 2010)
First author Ferrari, Maura
Pag. 232 di 300 31/08/2011 Optimization of cryopreservation protocols to maintain the quality of MSCs is an important task for stem cells
banks. To allow long-term storage, MSCs are slowly cooled and stored at -196°C in liquid nitrogen.
Unfortunately, despite the use of well standardized protocols, the percentage of living cells after thawing is
low. In order to use MSCs for regenerative medicine, it is important to find a cryopreservation solution able not
only to reduce cell death but also free of anima] proteins, in order to reduce zoonoses risk. Materials and
Methods - Rat, sheep (models) and horses MSCs were isolated from bone marrow and adipose tissue and in
vitro cultured. Each MSCs sample was frozen in three different cryoprotectant solutions (BioLife Solutions).
After thawing and re-seeding, cell viability was assessed for three consecutive days and cell counts were
performed at 24-h intervals. Results - Cell viability differences were observed not only among the three
different cryopreservation reagents used but also according to the species from which the MSCs were
derived. Equine MSCs were much more sensitive to the freezing process than rat and sheep derived cells.
Conclusions - The possibility for long-term storage for MSCs and other types of cells in a frozen state which
are suitable for immediate clinical application could provide immense benefit in regenerative medicine.
Moreover, identification of more effective cryoprotectant solutions deprived of animal proteins could improve
the quality of the freeze/thaw process and reduce cost/benefit ratio.
ID nr. 4391
Ferrari° M, Renzi° S, Sesso L, Cornali M, Carlin S
Banking of mesenchymal stem cells for rendon repair in race horses
Diversity in biobanking : embracing differences, harnessing commonalities : ISBER 2010 Annual Meeting &
Exhibits : May 11-14, 2010 Rotterdam, Netherlands / [s.l. : s.n., 2010]. - p 36
ISBER 2010 Annual Meeting & Exhibits : Rotterdam, Netherlands : May 11-14, 2010)
First author Ferrari, Maura
Mesenchymal stem cells (MSCs) are frequently used in veterinary medicine to repair orthopaedic injuries,
particularly in race horses. MSCs can be isolated from dìfferent tissues, but the most commonly used for
ciinical applications are derived from adipose tissue or bone marrow. A branch of our cell culture bank was
dedicated to the storage of MSCs, in order to treat tendon injuries in horses. Methods -MSCs have been
isolated from fat tissue and bone marrow, propagated in vitro not more than four passages and tested for
multi-potentiality. Before freezing, every batch was subjected to safety controls. Only the batches free from
contaminations (viruses, eubacteria and mycoplasma) were banked. MSCs were used not only for autologous
but also for allogeneic implantation. For implantation, celis were suspended in autologous Platelet Rich
Plasma (PRP) and inoculated into the damaged tendon. After MSCs treatment, horses were subjected to a
rehabilitative period and to ecographic controls. A sample of every batch was conserved in the bank to be
preserved either for a subsequent treatment or in case of complaint. Results - Currently the MSC bank
consists of 100 batches of celis. Each ampoule contains an average of 7x106 MSCs. Ecographic
examinations showed good tendon regeneration and some of the treated animals have started competitions.
Conclusions - The MSC bank at IZSLER is organized in accordance with quality parameters. The prepared
celis have shown to be useful for clinical application regarding tendon repair.
ID nr. 4394
Razzuoli° E, Dotti° S, Archetti° IL, Amadori° M
Clinical chemistry parameters of piglets at weaning are modulated by an oral, low-dose interferon-a
treatment
Atti Soc Ital Sci Vet. - Vol. 63 ( 2009). - cdrom p 447-449. - 6 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (63 : Udine : 16-18
Settembre 2009)
First author Razzuoli, Elisabetta
Clinical chemistry parameters were investigated in piglets weaned at 22 and 28 days. The effects of an oral,
low-dose interferon (IFN)-a treatment at weaning were evaluated as well. The trial was carried out on 59
Pag. 233 di 300 31/08/2011 piglets of the same farm, allocated to three groups: the first and the second groups were weaned at 28 and 22
days, respectively; the third one was weaned at 22 days and treated with IFN-a. Results indicate that early
weaning at 22 days implies higher efforts of environmental adaptation. In such animals an oral, low-dose IFNa treatment can effectively modulate the circuits of the inflammatory response, thus improving the homeostatic
response to the early weaning stressor.
ID nr. 4395
Vanni M, Intorre L, Barigazzi° G, Dottori° M
Antimicrobial susceptibility of 925 actinobacillus pleuropneumoniae strains isolated in swine from
1994 to 2008
Atti Soc Ital Sci Vet. - Vol. 63 ( 2009). - cdrom p 214-216. - 7 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (63 : Udine : 16-18
Settembre 2009)
The susceptibility to 26 antimicrobial agents was determined in 925 Actinobacillus pleuropneumoniae strains
isolated in swine from 1994 to 2008. Susceptibility to antimicrobials normally showing good activity, such as
florfenicol, fluoroquinolones and cephalosporins, remained relatively high in the considered period. However,
the study highlighted the occurrence of resistance towards therapeutic antimicrobials, such as penicillins,
tetracyclines, macrolides and cotrimoxazole. The emergence of A. pleuropneumoniae resistant to currently
available drugs underlines the importance of encouraging the prudent use of antimicrobials in swine
pleuropneumonia treatment.
ID nr. 4396
Dotti° S, Villa° R, Candotti° P, Lombardo° T, Vinco° LJ, Ferrari° M
Humoral and cell-mediated response evaluation in Duroc pigs after experimental infection with
Porcine respiratory and Reproductive syndrome virus (PRRSV)
Atti Soc Ital Sci Vet. - Vol. 63 ( 2009). - cdrom p 123-125. - 4 bib ref
Convegno Nazionale della Societa' Italiana delle Scienze Veterinarie (SISVET) (63 : Udine : 16-18
Settembre 2009)
First author Dotti, Silvia
The Respiratory and Reproductive Syndrome Virus (PRRSV) is one of the most studied virus in swine
pathology. At now, the knowledges about the real interaction virus-host are not clear and incomplete;
moreover, there are a lot of problems to reduce the economic losses in growing either in nursery phase. The
aim of this study was to evaluate the susceptibility to PRRSV infection of a pure-breed pig line, Duroc, based
on clinical, virological and immunological investigations. Ten 30 days old PRRS-free pigs were infected with
an Italian strain of PRRSV, BS/114/2000; others two animals were housed in a separate unit as control group.
The blood of all animals were collected every week and they were killed after 70 days. This experiment is the
latest step of a project that aims to investigate the possible different susceptibility between Large White,
Landrace and Duroc pure-breed lines to the PRRS virus.
ID nr. 4398
Menotta° S, D’antonio° M, Diegoli G, Montella L, Raccanelli S, Fedrizzi° G
Depletion study of PCDD/Fs and dioxin-like PCBs concentrations in contaminated home-produced
eggs: preliminary study
Anal Chim Acta. - Vol. 672 ( 2010). - p 50-54 - 31 bib ref
Impact factor: 3,757
Pag. 234 di 300 31/08/2011 First author Menotta, Simonetta
The presence of Zeranol (a-zearalanol) in urine samples due to natural contaminantion or illegal treatment is
under debate within the European Union. The simultaneous determination of zeranol, its epimer taleranol (ßzearalanol), zearalanone and the structurally-related mycotoxin zearalenone with the corresponding a- and ßzearalenol metabolites appears to be critical in deciding whether an illegal use has occurred. The aim of this
study is to develop and validate a simple analytical procedure applicable to bovine and swine urine samples
for the determination of all six resorcylic acid lactones. After an enzymatic deconjugation, the urine was
subjected to a one-step cleanup on a commercially available immunoaffinity chromatography cartridge. The
analytes were detected by liquid chromatography-negative-ion electrospray tandem mass spectrometry using
deuterium-labelled internal standards. The method was validated as a quantitative confirmatory method
according to European Commission Decision 2002/657/EC. The evaluated parameters were: linearity,
specificity, precision (repeatability and intra-laboratory reproducibility), recovery, decision limit, detection
capability and ruggedness. The decision limits (CCa) obtained, were between 0.56 and 0.68 µg L-1; recovery
above 66 % for all the analytes. Repeatability was between 1.4 % and 5.3 % and within-laboratory
reproducibility between 1.9 % and 16.1 % for the six resorcylic acid lactones.
ID nr. 4399
Menotta° S, D'Antonio° M, Santachiara F, Squintani G, Bassoli M, Boschetti L, Fedrizzi° G
Monitoring of DL PCBs and NDL PCBs in fishes caught from Po river in Emilia Romagna Region-Italy
6th International PCB Workshop : May 30 - June 2 2010, Visby, Sweden / [s.l. : s.n., 2010]. - p 140-141
International PCB Workshop (6th : Visby, Sweden : May 30 - June 2, 2010)
First author Menotta, Simonetta
ID nr. 4400
Fedrizzi° G, D'Antonio° M, Scandurra° S, Elanco° EL, Masotti° D, Padovani E, Vitali A,
Menotta° S
Distribution study of PCBs indicators in bovine organs and tissues from a contaminated farm
6th International PCB Workshop : May 30 June 2 2010, Visby, Sweden / [s.l. : s.n., 2010]. - p 143
International PCB Workshop (6th : Visby, Sweden : May 30 June 2 2010)
First author Fedrizzi, Giorgio
ID nr. 4401
Scordella G, Rubini° S, Bresolin R, Bolognesi° E, Menotta° S
Dioxins and Dioxin-like compounds in seafood: the lack of a shared monitoring plan
World Aquaculture 2009, a blue revolution to feed the world : September 25-29, 2009 Veracruz, Mexico /
World Aquaculture Society. - [s.l. : s.n, 2009]. - p 787
World Aquaculture 2009 : Veracruz, Mexico : September 25-29, 2009)
Persistent organic pollutants (POPs), such as dioxins, furans and PCBs (polychlorinated biphenyls) are a
group of toxic and persistent chemicals whose effects on human health and on the environment include
dermal toxicity, immunotoxicity, reproductive effects and teratogenicity, endocrine disrupting effects and
carcinogenicity (Matthews et al. , 2008). They are produced in small quantities in the manufacture of several
chlorinated compounds, in chlorine bleaching in pulp and paper mills and in processes of incineration and
combustion (Carro et al., 2008). The increasing presence in the environment of these substances, coupled
with several accidents (Yusho-Japan, Yu-cheng-Taiwan and Seveso-Italy), Have triggered a Jeep concern
from the international community for their reduction and control. Since dioxins are lipophilic compounds, they
accumulate in the food chain (De Mul et al., 2008). Bioaccumulation is continuing along the trophic chain and
releases go on from landfills, polluted soils or sediments. The dietary exposure to dioxins and dioxin-like
PCBs, exceeds the Tolerable Weekly Intake (TWI) or the Tolerable Daily Intake (TDI) for a considerable part
of the European population. The Scientific Committee on Food (SCF) of the EU adopted since 2001 an
Pag. 235 di 300 31/08/2011 opinion on the Risk Assessment of dioxins and dioxin-like PCBs in food. The levels of dioxins and dioxin-like
contaminants in fish and humans have decreased considerably during the past three decades, however the
dietary intakes of these compounds vary considerably between countries and between population groups
within countries. Limited data are still available concerning the levels and dietary intakes of POPs by seafood
consumption in many regions of the world. Because, in genera, fish and seafood are the food group that show
the highest levels of PCBs, is important to monitor these goods, also because fisheries products play an
important role in the European diet as a valuable source of protein and as a healthy food. The low
biodegradation rate of POPs in bivalve molluscs, makes that these chemicals are found in higher levels than
other marine organisms. Mussels and oysters are commonly used as bioindicators of these compounds,
because they may accumulate contaminants in their tissues higher concentrations than in the marine water.
From 2004 to 2008, the Dioxins Laboratory of the Marketable Animal Origin Foods of the Bologna IZSLER
had analyzed 33 samples of bivalve molluscs (coming from the North West Adriatic Sea; Italy), searching for
PCBs (18 congenera), Dioxins and PCB-Dioxin-like compounds. All the resulting data were below the EU risk
levels. Anyway many world references report hazard ratios for POPs registered on seafood coming from other
locations, indicating that these compounds may pose some health risks to the local population. A real shared
monitoring pain of these compounds should be improved in the market of seafood, supplying the consumer
also with information on the TDI and the TWI.
ID nr. 4403
Menotta° S, D’Antonio° M, Diegoli G, Montella L, Raccanelli S, Fedrizzi° G
Depletion study of PCDD/Fs and dioxin-like PCBs concentrations in contaminated home-produced
eggs: Preliminary study
4th International symposium on recent advances in food analysis : November 4–6, 2009, Prague, Czech
Republic : book of abstract / edited by Jana Pulkrabova, Marie Suchanova and Monika Tomaniova. - [s.l. :
s.n., c2009]. - Lm-22
International symposium on recent advances in food analysis (4th : Prague, Czech Republic : November
4–6, 2009)
First author Menotta, Simonetta
Dioxins (PCDD/Fs) and polychlorobyphenyls (PCB) are ubiquitous environmental contaminants. They are
very persistent to chemical degradation, and accumulate in the food chain. The contamination of food
products with dioxins and PCBs is a well studied issue, because food is generally considered as the major
source of dioxin intake for humans. Eggs, in particular, contain an high fat percentage, and may accumulate
persistent organic pollutants such as dioxin and PCBs. In Italy, the Regional Monitoring Plan used in the field
for 2009, has also included the control of environmental pollutants in small egg producers (so called homeproduced eggs). Following an irregular result, a private owner was recruited on a voluntary basis, and his
birds, that were contaminated on a first sample (12 hens), were transferred from their free-range farming
system into a lab controlled environment. Every day (from day 0 to 60) total eggs were collected: most of them
were analysed for the evaluation of dioxins, dioxin like PCBs (DL PCBs), and non dioxin like PCBs (NDL
PCBs six congeners) levels. The number of lay-eggs varied from four to 12 in a single day. All the eggs from
each week were homogenised and lyophilised. The fat fraction was extracted by accelerated solvent
extraction (ASE). The dioxins and PCBs contents were determined according to EPA 1613/94 rev B method
by gas chromatographic determination, coupled with high resolution mass spectrometry. The content of
PCDD/F, DL PCB and NDL PCB was evaluated by mean from week to week. The concentration of dioxins
was lower than DL PCBs (2,5 pgTEQ/g of fat against 4,5 pgTEQ/g), but we observed the same depletion
trend for both. After a steady level for the first two weeks, there was a small depletion until the sixth week,
were PCDD/F and DL PCB showed similar concentration. Then, while a continuous depletion was seen for
PCDD/F concentration, DL PCB levels decreased very slowly, and reached about 2 pgTEQ/g of fat. On the
opposite, NDL PCBs had a different course: there was an increase between week six and seven, but the
mean levels remained very low (about 20 ng/g of fat). The dioxins, and sum of dioxin and DL PCBs
concentration were below the fixed European limits ( i.e. 3 pgTEQ/g of fat for dioxins and 6 pgTEQ/g of fat for
sum of dioxins and DL PCB), beginning from the third week of trial, because of their removal from the
contaminated environment.
Pag. 236 di 300 31/08/2011 ID nr. 4404
Bacci ML, Fantinati P, Alborali° GL, Zannoni A, Penazzi P, Bernardini C, Forni M,
Ostanello F
Multilevel approach to study boar fertility in commercial farm
60th Annual meeting of the european association for animal production : August 24th - 27th 2009 Barcelona
Spain : book of abstracts no.15 / [s.l. : s.n., 2009]. - p 452
Annual meeting of the european association for animal production (60th : Barcelona, Spain : August 24th 27th, 2009)
Semen quality assessment represents a fundamental step for obtaining successful artificial insemination (AI)
in pig industries, however the decline in boar fertility, non related to apparent causes, is a common and
economically relevant problem. In commercial settings, the ejaculates were evaluated at collection, but
traditional quality estimates are not able to foretell fertility outcome. New fertility parameters have been
therefore studied in vitro (Popwell and Flowers, 2004; Turba et al., 2007) and compared with traditional ones.
The present research aimed to study the causes of fertility decline in boars not bound to clinical signs of
disease, utilizing various approaches: study of in vitro fertility with traditional and new parameters, study of in
vivo fertility and study of health status of subjects. Therefore nine boars of proven fertility have been
monitored for 5 months from March and sperm and blood samples have been repeatedly collected for seminal
and serological evaluations. At this level we researched ADV, PRRSV, PCV2, SIV (H1N1, H2N1, H3N2)
antibodies. In order to evaluate boar fertility we utilized in vitro (motility, viability, acrosome condition,
mitochondrial membrane potential, etc.), as well as in vivo parameters (Farrowing Rate and Litter Size
outcome of 230 Artificial Insemination). The low percentage (<5%) of damaged acrosome inan ejaculate
significantly correlates with high LS. On the contrary no correlations have been found among seroconversions
for PRRSV (2 boars) and for ADV (2 boars) and in vivo fertility as well as positivity for SIV (H1N2 strain) (4
boars). This research was supported by grants from Bologna University (RFO 60%)Semen quality
assessment represents a fundamental step for obtaining successful artificial insemination (AI) in pig
industries, however the decline in boar fertility, non related to apparent causes, is a common and
economically relevant problem. In commercial settings, the ejaculates were evaluated at collection, but
traditional quality estimates are not able to foretell fertility outcome. New fertility parameters have been
therefore studied in vitro (Popwell and Flowers, 2004; Turba et al., 2007) and compared with traditional ones.
The present research aimed to study the causes of fertility decline in boars not bound to clinical signs of
disease, utilizing various approaches: study of in vitro fertility with traditional and new parameters, study of in
vivo fertility and study of health status of subjects. Therefore nine boars of proven fertility have been
monitored for 5 months from March and sperm and blood samples have been repeatedly collected for seminal
and serological evaluations. At this level we researched ADV, PRRSV, PCV2, SIV (H1N1, H2N1, H3N2)
antibodies. In order to evaluate boar fertility we utilized in vitro (motility, viability, acrosome condition,
mitochondrial membrane potential, etc.), as well as in vivo parameters (Farrowing Rate and Litter Size
outcome of 230 Artificial Insemination). The low percentage (<5%) of damaged acrosome inan ejaculate
significantly correlates with high LS. On the contrary no correlations have been found among seroconversions
for PRRSV (2 boars) and for ADV (2 boars) and in vivo fertility as well as positivity for SIV (H1N2 strain) (4
boars). This research was supported by grants from Bologna University (RFO 60%).
ID nr. 4406
Harouna A, Zecchini M, Locatelli C, Scaccabarozzi L, Cattaneo C, Amadou A, Bronzo V,
Marichatou H, Boettcher PJ, Zanoni° MG, Alborali° L, Moroni P
Milk hygiene and udder health in the periurban area of Hamdallaye, Niger
Trop Med Int Health. - Vol. 41 ( 2009). - p 705-710. - 16 bib ref
Impact factor: 2,312
The prevalence of intra-mammary infections in dairy herds was studied in Hamdallaye, Niger. A total of 956
milk samples were collected in 2007 from 239 lactating cows of four local breeds in eight traditional herds; the
first sampling was undertaken in the dry season at morning milking, and the second in the rainy season at
evening milking. Staphylococcus aureus, Coagulase-Negative Staphylococci (CNS) and environmental
Pag. 237 di 300 31/08/2011 microorganisms were detected in significantly (p<0.05) more samples in the rainy season, 55.2%, than in the
dry season, 27.1%. Statistically significant (P<0.05) differences in prevalence were observed among herds
and according to lactation number. Infections were assigned to four classes, according to the major pathogen,
and the respective mean somatic cell counts during the dry season were: S. aureus, 775×103 cells/ml; CNS,
447× 103 cells/ml; environmental microorganisms, 407×103 cells/ml; and non-infected, 262×103 cells/ml.
Most of the tested strains were sensitive to antibiotics, and selected strains of S. aureus (n=15) were negative
to the multiplex PCR tests for production of enterotoxins.
ID nr. 4407
Angelucci G, Fenza A, Viale I, Rolesu S, Alborali° PL, Salati F
Evolution of finfish culture and diseases in Sardinia, Italy
14th EAFP International Conference : Diseases of fish and shellfish : September 14-19, 2009 Prague / [s.n. :
s.l., 2009]. - p 375
EAFP International Conference (14th : Prague : September 14-19, 2009)
Anguilla anguilla, rainbow trout Oncorhynchus mykiss and, recently, meagre Argyrosomus regius are now
reared in sea-cages and/or in land-based plants. In particular, the production of reared fish in Sardinia has
increased from 300 t in 1994 to 1,000 tons in 2000 to about 3,000 tons in 2006 and, keeping the same
production level until today, raised the highest place among sea-cages production in Italian
aquaculture.During this period, the diseases showed a parallel increase together with the intensive
production. Regarding bacterial diseases of marine fish during the period 2001-200a, the highest incidence of
infections was caused by TenacibacuIum maritimurn responsible for marine flexibacteriosis; moreover,
outbreaks of diseases caused by Vibrio spp., Photobacteriurn (Pasteurella) piscicida, and Schewanella
(Pseudomonas) anguilliseptica occurred almost every year. In cultured ell, infection by Flexibacter colurnnaris,
responsible for freshwater flexibacteriosis was the most often diagnosed bacterial disease. Among viral
diseases, Viral Encephalopathy and Retinopathy (VER or VNN) of sea bass and Lymphocystis of sea bream
were irregularly recorded. Regarding parasitic diseases, Arnyloodiniurn ocellaturn and Ceratornyxa sp. were
constantly reported in marine fish. However, Atrispinum sp. caused heavy losses particularly in Sharp-snouted
bream and, in the last years, Sparicotyle sp. infections hit cultured Gilthead sea bream and meagre with high
mortalities. Moreover, Dactylogyrus sp. and Anguillicola sp. were constantly recorded in cultured eel..
ID nr. 4408
Chiari° M, Zanoni° MG, D’Incau° M, Salogni° C, Alborali° B
Isolation of Salmonella spp. in wild boars (sus scrofa) from Northern Italy
III Convegno Nazionale di Ecopatologia della Fauna Torino SIEF, 15-17 Ottobre 2009 : atti / [s.l. : s.n.,
2009]. Convegno Nazionale di Ecopatologia della Fauna (3. : Torino : 15-17 Ottobre 2009)
First author Chiari, Mario
The health status of wildlife is a common concern of different stakeholders: the veterinary and public services
for the increasing number of infectious diseases and zoonosis, shared between wildlife and domestic animals;
the public administrators for management reasons; and the hunters for a direct interest in hunter-harvesting.
Since 1997 a health monitoring on wildlife in Brescia Province has been applied with the informal cooperation
of the hunters’ associations. Starting on hunting season 2006-07, an agreement with the common aim to a
better understanding of health and disease in free-ranging wildlife was officially established between the
veterinary services, the public administrators and the hunter associations. The faeces and viscera collected by
the hunters during three hunting seasons (starting on 2006-07) were delivered to the Brescia laboratory for a
full set of diagnostic examinations. In particular, Salmonella was isolated by faeces following the methods
reported in “Annex D ISO 6579:2002”, mandatory in the implementation of Salmonella monitoring and control
plan for primary productions. This method was applied in parallel with home-made isolation procedure based
on an enrichment phase (Rappaport-Vassiliadis Broth) and plating (Hecktoen enteric agar). Salmonella
Pag. 238 di 300 31/08/2011 identification was performed using biochemical tests and serotyping. Isolated strains of S. typhimurium and S.
enteritidis were also phagetyped. From 1228 investigated samples, 292 strains of salmonella were isolated.
The results revealed a significant prevalence of isolations of serotypes pathogenic to humans as well as
serotypes not considered pathogenic. Figures on serotypes isolated from wild boars do not reflect prevalence
data on the isolates in domestic species in our territory. The most frequently detected salmonella were
serotypes Coeln, Ball and Thyphimurium of S. enterica ssp. enterica. Other isolates, less frequently detected,
belong to S. enterica ssp. diarizonae and S. enterica ssp. houtenae, which are usually found only in coldblooded animals. Over 50% of S. thyphimurium isolates were phagotyped as DT104. Salmonella typhimurium,
a potential risk to human health, is sporadically but constantly detected in wild boar population. This aspect, in
conjunction with the large size of population of wild boars in Brescia province and the increasing numbers of
hunters specialized on this species, make the veterinary inspection and laboratory control of hunted wild
boars an absolute need in the future hunting seasons.
ID nr. 4410
Carra° E, Bassi° S, Bergamini° F, Sarti M, Colmegna° S, D’Incau° M, Fontana MR,
Lambertini A, Pongolini° S
Salmonella enterica subsp. enterica serovar Manhattan in humans: a collection of strains makes the
difference
International Symposium Salmonella and Salmonellosis : June 28-30, 2010 Saint-Malo, France :
proceedings / [s.l. : s.p., 2010]. - p 389-390
International Symposium Salmonella and Salmonellosis : Saint-Malo, France : June, 28-30, 2010)
First author Carra, Elena
ID nr. 4411
Cecchinato M, Ceolin C, Busani L, Dalla_Pozza M, Terregino C, Moreno° A, Bonfanti L,
Marangon S
Low pathogenicity avian influenza in Italy during 2007 and 2008: epidemiology and control
Avian Dis. - Vol. 54 n. 1 ( 2010). - p. 323-328. - 20 bib ref
Impact factor: 2,003
Project PRF2006304 - Prevalance of avian and swine influenza virus infection among farmers and animals.
- Vonesch Dr.ssa Nicoletta - UO - MINSAN - 2006 - 300000,00 EUR - 02/01/2008 0.00.00 02/01/2011 0.00.00 - 1 - G2 - Reparto di Batteriologia Specializzata - Cordioli Dr. Paolo - I
Since 1999, the Italian poultry production system has experienced several outbreaks of avian influenza (AI),
mainly located in northeastern Italy. This paper describes the low pathogenicity (LP) AI outbreaks detected
during the surveillance activities implemented in 2007–08. From May to October 2007, ten rural and hobby
poultry farms were infected by an LPAI virus of the H7N3 subtype. In August–October 2007, the H7N3 LPAI
virus was introduced into the industrial poultry sector with the involvement of six meat turkey farms.
Phylogenetic analysis of the hemagglutinin gene indicated that all but one of the H7N3 virus strains had a high
level of homology (98.7%–99.8%). Furthermore, in August 2007, an LPAI H5N2 virus was identified in a freerange geese and duck breeder flock. The hemagglutinin and neuraminidase genes showed a high level of
homology (99.8% and 99.9%, respectively) with H5N2 LPAI viruses isolated from mallards in July 2007 in the
same area, suggesting a possible introduction from the wild reservoir. All the birds (in total 129,386) on the
infected poultry farms were culled. The prompt implementation of AI control measures, including the
enforcement of a targeted emergency vaccination plan, allowed the rapid eradication of infection. In 2008,
three LPAI viruses (two H7N1 and one H5N1) were identified in dealer/rural farms. The surveillance activity
implemented in this area allowed the prompt detection of LPAI viruses of the H5 and H7 subtypes in the rural
sector, which, as observed in the 2007 epidemic, might be the source of infection for industrial poultry..
ID nr. 4413
Pag. 239 di 300 31/08/2011 Daminelli° P, Finazzi° G, De_Nadai° V, Oliverio° E, Ducoli° S, Bonometti° E, Gregorelli°
M, Boni° P
Behaviour of Listeria monocytogenes in delicatessen products: seafood salad
Ind Aliment (Pinerolo). - Vol. 49 no 504 ( 2010). - p 22-28. - 6 bib ref
First author Daminelli, Paolo
Seafood salad from four different Food Companies has been contaminated with a three strains suspension of
Listeria monocytogenes. Samples were kept at different temperatures to evaluate pathogen behaviour during
shelf-life. Observed data showed that Listerias multiplication in seafood salad can be counteracted by different
food factors as pH < 5 and presence of endogenous lactic flora able to bio-compete aganist pathogen. On the
contrary, without these factors, this kind of food supports a quick increase of Listeria monocytogenes.
ID nr. 4416
Bolzoni° G, Marcolini° A
Bactoscan FC - project for unified conversion line in Italy
Milchwissenschaft. - Vol. 65 no 3 ( 2010). - p 309-310
Impact factor: 0,4060
Project PRC2009015 - Development of an information system for risk analysis in raw milk production. Bolzoni Dr. Giuseppe - Solo - MINSAN - 2009 - 169900,00 EUR - 18/10/2010 0.00.00 17/10/2012 0.00.00 - 1 - V6 - REPARTO PRODUZIONE PRIMARIA - Bolzoni Dr. Giuseppe - I
2 - V6 - REPARTO PRODUZIONE PRIMARIA - Zanardi Dr. Giorgio - I
3 - T0 - DIREZIONE SANITARIA - Pongolini Dr. Stefano - I
4 - T0 - DIREZIONE SANITARIA - Bellini Dr.ssa Silvia - I
5 - M2 - Sezione diagnostica provinciale di Bergamo - Tranquillo Dr. Vito - I
6 - N6 - Sezione diagnostica provinciale di Piacenza - Garbarino Dr.ssa Chiara - I
7 - T0 - DIREZIONE SANITARIA - Tamba Dr. Marco - I
First author Bolzoni, Giuseppe
ID nr. 4417
Candotti° P, Zappulla D, Archetti° I, Rota_Nodari° S
Use of paper strips as nesting material in sows: effects on abnormal behaviours, salivary cortisol
and productive data
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 296. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
Project PRC2008014 - Influence of environmental conditions on the welfare of some farm animal species
(cattle, swine,turkeys,laying hens, goats) in diverse Italian herds. - Vinco dr. Leonardo James - Solo
- MINSAN - 2008 - 140000,00 EUR - 01/09/2009 0.00.00 - 31/08/2011 0.00.00 - 1 - G3 Reparto benessere animale, immunoprofilassi, allevamenti e sperim. Animale - Vinco dr. Leonardo
James - I
2 - N3 - Sezione diagnostica provinciale di Forlì - Tosi Dr. Giovanni - I
3 - M2 - Sezione diagnostica provinciale di Bergamo - Gaffuri Dr.ssa Alessandra - I
4 - I4 - Reparto produzioni zootecniche e sorveglianza epidemiologica degli allevamenti Bertocchi Dr. Luigi - I
First author Candotti, Paolo
ID nr. 4418
Morandi F, Bacci B, Panarese S, Ferrara D, Fusaro L, Bacci ML, Dottori° M, Bonilauri° P,
Leotti G, Vila T, Joisel F, Ostanello F, Sarli G
Conventional sows inseminated with artifi cially PCV2-infected semen: II. Post mortem results
Pag. 240 di 300 31/08/2011 21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 283. - 8 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4419
Martelli P, Bonilauri° P, Gozio S, Cevidalli AE, Guazzetti S, Caleffi A, Borghetti P
The effect on PCV2 viremia in pigs vaccinated with a single dose of Porcilis PCV®
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 406. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4421
Luppi° A, Bonilauri° P, Mazzoni C, Di_Lecce R, Dottori° M
Swine herd with a prevalent PCV2 subclinical infection: diagnostic investigation results before and
after PCV2 vaccination
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 432. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
First author Luppi, Andrea
ID nr. 4422
Candotti° P, Rota_Nodari° S, Nassuato° C
Serum endotoxin in pigs aff ected by wasting syndromes
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 457. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
First author Candotti, Paolo
ID nr. 4423
Sarli G, Morandi F, Panarese S, Bacci B, Ferrara D, Fusaro L, Bacci ML, Galeati G,
Dottori° M, Bonilauri° P, Lelli° D, Leotti G, Vila T, Joisel F, Ostanello F
Conventional sows inseminated with artificially PCV2 infected semen: I. In vivo results
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 458. - 10 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4424
Alborali° L, Pavesi° R, Gradassi° M, Sarli G, Zanoni° M, Salogni° C, Giovannini° S
Co-infections in PMWS field cases in Italian herds
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 463. - 11 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
First author Alborali, Giovanni Loris
ID nr. 4425
Alborali° LG, Gradassi° M, Pavesi° R, Boniotti° B, Nassuato° C, Giovannini° S, Giacomini°
E, Bellini° S, Pacciarini° M
Pag. 241 di 300 31/08/2011 Detection of PRRSV in oral fluid samples: longitudinal study under field conditions
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 483. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
First author Alborali, Giovanni Loris
ID nr. 4426
Dotti° S, Sandri G, Villa° R, Razzuoli° E, Sossi° E, Amadori° M
Time-course of the immune response to Porcine Reproductive and Respiratory Syndrome Virus
(PRRSV) in giltss
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 490. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
Project PRRSV AUTOFINANZIATO - Evaluation of the immune response to Porcine Respiratory and
Reproductive syndrome virus (PRRSV) - Ferrari Dr.ssa Maura - Solo - IZSLER - 2009 - 75000,00
EUR - 31/07/2009 0.00.00 - 31/12/2010 0.00.00 - 1 - K4 - REPARTO SUBSTRATI CELLULARI E
IMMUNOLOGIA CELLULARE - Ferrari Dr.ssa Maura - I
2 - M1 - Sezione diagnostica provinciale di Brescia - Alborali Dr. Loris - I
First author Dotti, Silvia
ID nr. 4427
Alborali° LG, Gradassi° M, Zanoni° M, Moreno_Martin° A, Catella° A, Salogni° C, Sozzi°
E, Foni° E, Cordioli° P
Pandemic influenza virus (A/ H1N1) outbreak in pig farm in north of Italy
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 585. - 8 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
Project PRF2006304 - Prevalance of avian and swine influenza virus infection among farmers and animals.
- Vonesch Dr.ssa Nicoletta - UO - MINSAN - 2006 - 300000,00 EUR - 02/01/2008 0.00.00 02/01/2011 0.00.00 - 1 - G2 - Reparto di Batteriologia Specializzata - Cordioli Dr. Paolo - I
First author Alborali, Giovanni Loris
ID nr. 4428
Magistrali C, Cucco L, D’Angelo G, Gherpelli° Y, Bonilauri° P, Merialdi° G
Comparison between two different methods for antimicrobial susceptibility testing of Brachyspira
hyodysenteriae
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 735. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4429
Mazzoni C, Raffi V, Rosignoli° C, Bonilauri° P, Spaggiari° B, Gelmetti° D, Gibelli° L, Maioli°
G, Faccini° S, Dottori° M, Tonon F, Luppi° A
Clostridiosis in the farrowing room with an atypical onset: a clinical case
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 791. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
Project PRC2001006 - Use of PCR for typing C. perfrigens strains isolated from enteric and entero-toxiemic
disease cases of animals and animal source foods. - Rosignoli Dr. Carlo - Solo - MINSAN - 2001 -
Pag. 242 di 300 31/08/2011 93163464,00 LIT - 03/03/2003 0.00.00 - 02/07/2004 0.00.00 - 1 - M6 - Mantova - Rosignoli Dr.
Carlo - I
ID nr. 4430
Luppi° A, Iodice G, Bonilauri° P, Merialdi° G, Merenda° M, Dottori° M
Simultaneous infection by serotypes 4 and 12 of Haemophilus parasuis in a swine herd: a clinical
case
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 826. - 5 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4431
Bresaola M, Lombardo° T, Villa° R, Sossi° E, Ferrari° M
Retrospective study on swine Torque Teno virus genogroups 1 and 2 and porcine circovirus type 2
coinfection in Italian pig sera (from 1990 to 2009)
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 884. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4432
Galletti° E, Merialdi° G, Antonelli A, Brini E, Fusaro L, Sarli G, Fontana° MC, Spaggiari° B,
Martelli P
Isoimmune thrombocytopenic purpura in newborn piglets: a case report
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 909. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
First author Galletti, Elena
ID nr. 4433
Razzuoli° E, Villa° R, Sossi° E, Dotti° S, Amadori° M
The interferon-alpha response in piglets at weaning
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 920. - 5 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
Project PRF2006201 - Adaptation of farm animals: repercussions on disease occurrence and drug usage Amadori Dr. Massimo - Capofila - MINSAN - 2006 - 861300,00 EUR - 15/01/2008 0.00.00 15/12/2010 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
2 - IZSSA - Nicolussi dr.ssa Paola - E
3 - Università degli Studi della Tuscia (Dipartimento di Produzioni Animali) - Lacetera dr. Nicola - E
4 - IZSME - Martucciello dr.ssa Alessandra - E
5 - IZSUM - Moscati dr.ssa Livia - E
6 - IZSLT - Cavallina dr.ssa Roberta - E
7 - IZSVE - Stefani dr.ssa Anna-Lisa - E
8 - Istituto Superiore di Sanità - Vitale dr. Augusto - E
9 - Università Cattolica del Sacro Cuore - Facoltà di Agraria - Istituto di Zootecnica (Piacenza) Trevisi dr. Erminio - E
10 - UNIVERSITA' MILANO - DIPARTIMENTO MEDICINA VETERINARIA EX ISTITUTO DI
ZOOTECNICA - Verga Dr.ssa Marina - E
First author Razzuoli, Elisabetta
Pag. 243 di 300 31/08/2011 ID nr. 4434
Cerati C, Palese A, Gamba F, Benaglia P, Bonilauri° P, Nisoli L
Assessment of sow births synchronised with oxytotocin and long-acting oxytocin (carbetocin)
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 1106. - 3 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4435
Mazzoni C, Borri E, Raffi V, Bertacchini S, Bonilauri° P, Palese A, Nisoli L
Randomised double blind comparison of oxytocin and long acting oxytocin (carbetocin) in the
synchronisation of farrowing in batch-breeding sows: evaluation of production parameters
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 1107. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4436
Bosi P, Merialdi° G, Bardasi° L, Scandurra S, Vecchi M, Messori S, Ferro P, Nisi I, Casini
L, Trevisi P
Effect of three different antibiotics on commensal intestinal microflora and on some productive traits
of weaning piglets
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 1029. - 2 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
ID nr. 4437
Razzuoli° E, Dotti° S, Villa° R, Sossi° E, Candotti° P, Amadori° M
Oral interferon-alpha treatment and environmental adaptation of swine
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 1172. - 5 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
Project PRF2006201 - Adaptation of farm animals: repercussions on disease occurrence and drug usage Amadori Dr. Massimo - Capofila - MINSAN - 2006 - 861300,00 EUR - 15/01/2008 0.00.00 15/12/2010 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
2 - IZSSA - Nicolussi dr.ssa Paola - E
3 - Università degli Studi della Tuscia (Dipartimento di Produzioni Animali) - Lacetera dr. Nicola - E
4 - IZSME - Martucciello dr.ssa Alessandra - E
5 - IZSUM - Moscati dr.ssa Livia - E
6 - IZSLT - Cavallina dr.ssa Roberta - E
7 - IZSVE - Stefani dr.ssa Anna-Lisa - E
8 - Istituto Superiore di Sanità - Vitale dr. Augusto - E
9 - Università Cattolica del Sacro Cuore - Facoltà di Agraria - Istituto di Zootecnica (Piacenza) Trevisi dr. Erminio - E
10 - UNIVERSITA' MILANO - DIPARTIMENTO MEDICINA VETERINARIA EX ISTITUTO DI
ZOOTECNICA - Verga Dr.ssa Marina - E
First author Razzuoli, Elisabetta
ID nr. 4438
Cavadini° P, Botti° G, Barbieri° I, Lavazza° A, Capucci° L
Molecular characterization of SG33 and Borghi vaccines used against myxomatosis
Pag. 244 di 300 31/08/2011 Vaccine. - Vol. 28 no 33 ( 2010). - p 5414-5420. - 24 bib ref
Impact factor: 3,616
Project PRC2003020 - Development of advanced laboratory methods for diagnosis and typing of rabbit
mixoma virus - Capucci Dr. Lorenzo - Capofila - MINSAN - 2003 - 90000,00 EUR - 26/04/2004
0.00.00 - 25/10/2006 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Capucci Dr. Lorenzo - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza Dr. Antonio - I
3 - IZSVE - Agnoletti Dr. Fabrizio - E
PRC2008003 - Development of molecular methods (or approaches) to characterize the DNA of
myxoma virus field isolates - Cavadini dr.ssa Patrizia - Solo - MINSAN - 2008 - 01/09/2009
0.00.00 - 31/08/2011 0.00.00 - 58000,00 EUR 1 - H2 - Reparto di Biologia Molecolare - Cavadini
dr.ssa Patrizia - I
First author Cavadini, Patrizia
Myxoma virus is a poxvirus responsible for myxomatosis in European Rabbits (Oryctolagus cuniculus). The
entire genome of the myxoma virus has been sequenced, allowing a systemic survey of the functions of a
large number of putative pathogenic factors that this virus expresses to subvert the immune and inflammatory
pathways of infected rabbit hosts. In Italy, industrial rabbits are mostly vaccinated against myxomatosis using
the attenuated myxoma virus strains Borghi or SG33. We have identified genetic markers specific for Borghi
or SG33 vaccine strains and established a PCR-based assay that could be used to: (a) rapidly diagnose the
presence of myxoma virus in infected organs; (b) discriminate between field strain-infected and vaccinated
rabbits and (c) differentiate between Borghi or SG33 vaccine strain.
ID nr. 4439
Menotta° S, D'Antonio° M, Padovani A, Fedrizzi° G
Determination of cadmium in edible tissues of crabs catch in italian teritorial waters: monitoring on
Emilia Romagna Regiong
XX Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) : qualità dell'ambiente e sicurezza
alimentare : 16, 17 18 Giugno 2010 Capanello su Staletti (CZ) / [s.l. : s.n., 2010]. - p 74
Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) (20 : Capanello su Staletti (CZ) : 16,
17 18 Giugno 2010)
First author Menotta, Simonetta
On 2008 there was a Community alert concerning the presence of Cadmium in crabs and on December 2009
there was a note from the Italian Ministry of Health concerning the control of cadmium in edible tissues of
crabs caught in Italian territorial waters. This laboratory analyzed a total of 32 samples of crabs by atomic
absorption spectroscopy graphite stove dividing the crustaceans in the following tissues: bright parts
contained in claws, dark parts contained in the shell and red parts contained in the shell. The results showed
that gastrointestinal shellfish tissues is at greatest risk, while the bright parts contained in claws are always
below the limit set by European legislation (Reg. l 881/2006 and following). Furthermore it is clear that the
crustaceans caught in the northern Adriatic present values of cadmium significantly lower compared to
shellfish from France and UK.
ID nr. 4440
Saccares S, Migliazzo A, Montagna C, Comin D, Mioni R, Decastelli L, Colmegna° S,
Cenci T, Tonucci F, Morena V, Marozzi S, Lanni L
Catering: critical aspects and microbiological risks identified by a collaborative study between
Istituti Zooprofilattici
XX Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) : qualità dell'ambiente e sicurezza
alimentare : 16, 17 18 Giugno 2010 Capanello su Staletti (CZ) / [s.l. : s.n., 2010]. - p 77
Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) (20 : Capanello su Staletti (CZ) : 16,
Pag. 245 di 300 31/08/2011 17 18 Giugno 2010)
The purpose of the collaborative study of several Istituti Zooprofilattici is the harmonization of the
documentation, of methods and investigations and assessment criteria for the development of the activities
previewed from HACCP in catering. Many authors have highligllted some problems that does not allocate, in
some cases, to perform risk assessments to different production processes. The main critical aspect emerged
during the meetings is the difficulty of comparing data between the different structures involved because they
lack uniform categorization of the many types of recipes resulting in considerately difficulty in identified
microbiological limits of acceptability for themselves. Therefore it was decided to make a careful survey in
order to propose a categorization of most-used recipes.
ID nr. 4441
Liuzzo G, Bonilauri° P, Leonelli R , Serraino A, Bentley S
Preliminary considerations on Sushi as potentially hazardous food
XX Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) : qualità dell'ambiente e sicurezza
alimentare : 16, 17 18 Giugno 2010 Capanello su Staletti (CZ) / [s.l. : s.n., 2010]. - p 39
Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) (20 : Capanello su Staletti (CZ) : 16,
17 18 Giugno 2010)
The Authors studied physicochemical properties (pH and AW) of samples of Nigiri Sushi and their ingredients
along their shelf lite, integrating those results with a predictive microbiolol model, in order to determine or to
ride out the growth of Listeria monocytogenes above the thresholds set by Reg.(EU) 2073/2005. Results point
towards substantial containment of the target biological hazard, even thouerh the prevention of thermal abuse
is a keypoint in increasing safet.
ID nr. 4442
Liuzzo G, Riu R, Merialdi° G, Bardasi° L, Galletti° G, Carra° E, Rosmini R, Giacometti F,
Pizzamiglio V, Serraino A
Indicator and pathogenic microorganisms contamination of hide of cattle presented to for slaughter
and contamination of the resultant carcasses
XX Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) : qualità dell'ambiente e sicurezza
alimentare : 16, 17 18 Giugno 2010 Capanello su Staletti (CZ) / [s.l. : s.n., 2010]. - p 6
Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) (20 : Capanello su Staletti (CZ) : 16,
17 18 Giugno 2010)
Foodborne pathogens including Escherichiaa coli 0157:H7, Salmonella spp. and Campylobacte rspp., can
enter the meat chain at multiple points. Animals with excessively dirty hides will represent a risk of crosscontaminations during transport, in the slaughtered house environment and during dressing procedures. The
aim of this work was to investigate the relationship among hygiene indicator microorganisms
(Enterobacteriaceae and Escherichiaa coli) count, in hide and carcass of cattle presented for slaughter, and
different hide cleanliness level in two abattoir (R and F) and estimate the prevalence of pathogens
Escherichiaa coli O l 57:H7, Salmonella spp. and Campylobacter spp. in hide and carcass of dirty animals.
The results showed that hide Escherichiaa coli and Enterobacteriaceae count were significantly higher in dirty
animals than in clean animals (P<0,05), but carcass contamination avere significantly higher in dirty animals
than in clean animals only in R abattoir. Escherichiaa coli O 157:H7 was detected in 19 samples of hidc (40,4
%), and 2 samples of carccass. Campylobacter jejuni as isolated in l3 samples of hidc (25,5%) and one
sample of carcass. Salmonella spp. were not found in all the samples of hide and carcass.
ID nr. 4443
Pag. 246 di 300 31/08/2011 Oliverio° E, Finazzi° G, Daminelli° P, Monastero° P, Boni° P
Dynamics of Listeria monocytogenes, Salmonella typhimurium Escherichia coli O157: H7 during the
production of 'Nduja
XX Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) : qualità dell'ambiente e sicurezza
alimentare : 16, 17 18 Giugno 2010 Capanello su Staletti (CZ) / [s.l. : s.n., 2010]. - p 14
Convegno Nazionale Associazione Italiana Veterinari Igienisti (AIVI) (20 : Capanello su Staletti (CZ) : 16,
17 18 Giugno 2010)
First author Oliverio, Elisa
The aim of this survey was to evaluate Listeria monocytogenes, Salmonella typhimurium and Escherichia coli
O157:H7 fate during production process of `Nduja, traditional italian Salami. For this purpose the kneading for
processing provided by Salumificio artigianale F.lli Pugliese was contaminated with mixtures of these
microorganisms, then bagged and seasoned according to the processing of the producer. The results of this
challenge test showed that the seasoning of the product reduces the concentration of microorganisms
artificially added.
ID nr. 4445
Razzuoli° E, Dotti° S, Archetti° IL, Amadori° M
Clinical chemistry parameters of piglets at weaning are modulated by an oral, low-dose interferon-a
treatment
Vet Res Commun. - Vol. 34 suppl 1 ( 2010). - p 189-192. - 6 bib ref
Impact factor: 1,050
Project PRC2005002 - Assessment and modulation of the inflammatory effects of early weaning in the piglet
- Amadori Dr. Massimo - Solo - MINSAN - 2005 - 22000,00 EUR - 15/05/2006 0.00.00 14/11/2008 0.00.00 - 1 - G3 - Reparto benessere animale, immunoprofilassi, allevamenti e sperim.
Animale - Amadori Dr. Massimo - I
First author Razzuoli, Elisabetta
Correspondig author Amadori, Massimo
Clinical chemistry parameters were investigated in piglets weaned at 22 and 28 days. The effects of an oral,
low-dose interferon (IFN)-a treatment at weaning were evaluated as well. The trial was carried out on 59
piglets from the same farm, allocated to three groups: the first and the second groups were weaned at 28 and
22 days of age, respectively; the third group was weaned at 22 days and orally treated at weaning with IFN-a
at a low dose (1 IU human lymphoblastoid IFN-a /kg body weight in drinking water) for 10 consecutive days.
The results of the field trial confirmed that weaning is one of the main stressing events for pigs at intensive
farms. In particular, these findings are based on a dramatic increase in serum haptoglobin levels after
weaning in the three groups under study. Results also indicated that early weaning at 22 days implies higher
environmental adaptation. In such animals, an oral, low-dose IFN-a treatment gave rise to a peculiar,
negative, acute-phase response (reduced levels of serum albumin) and to significantly lower a-globulin
concentrations in sera. Taken together, IFN-a was shown to modulate inflammatory responses to early
weaning stress.
ID nr. 4450
Pistone D, Pajoro° M, Fabbi° M, Vicari° N, Marone P, Genchi C, Novati S, Sassera D,
Epis S, Bandi C
Lyme borreliosis, Po river valley, Italy
Emerg Inf Dis. - Vol. 16 no 8 ( 2010). - p 1289-1291. - 14 bib ref
Impact factor: 6,794
Pag. 247 di 300 31/08/2011 We aimed to determine the presence of Ixodes ricinus ticks in heavily populated areas of the Po River Valley
after report of a Lyme disease case. Eighteen percent of ticks examined from 3 locations were positive for
Lyme disease borreliae. Lyme disease was diagnosed for 3 workers at risk for tike bite.
ID nr. 4451
Daffara S, Brazzelli V, Ronzi G, Vicari° N, Barbarini D, Pistone D, Pajoro° M, Fabbi° M,
Bandi C, Marone P
Chronic erythema and fish for salmon in Alaska: an enigma resolved by the laboratory of
microbiology
Microbiol Med. - Vol. 25 no 1 ( 2010). - p 70-71. - 5 bib ref
A 50-year-old man, pentathlete and fond of salmon fishing, after returning from Alaska, was referred to our
Division for an erythematous and indolent lesion on the right thigh gradually enlarging from the right knee to
the right rear and the buttock. It has been given a clinical diagnosis of figurate erythema. Laboratory exam
showed moderate leukocytosis and hypergammaglobulinemia, and the serological markers of past contact
with noted EBV and CMV. A "punch" biopsy of the edge of the lesion showed a superficial and deep
perivascular and interstitial infiltrate of lymphocytes, eosinophiles and a few plasma cells, consistent with a
borrelial infection. Lyme serology (IFI) showed a questionable borderline positivity; subsequent investigations
by ELISA and Western Blot were both positive and led to a diagnosis of erythema chronicum migrans in
course of borreliosis. The diagnosis is further confirmed by positive PCR for Borrelia burgdorferi sensu lato.
The erythema resolved after a 2-week doxycycline treatment (2x100 mg die). The amplification product (16S
rDNA gene) obtained from the skin biopsy, has been sequenced using standard ABI technology, confirming
the identification of a member of Borrelia burgdorferi sensu lato (sl) complex. Since this sequence was not
useful to identify the genospecies, further analysis were performed employing a nested PCR targeted on the
internal transcribed spacer (ITS) of Borrelia burgdorferi, following a protocol previously described1. The ITS
sequence showed 100% identity with Borrelia afzelii. Since this species is not reported from North America we
state that the patient has not been infected by Borrelia afzelii through a tick bite during the fishing trip in
Alaska.
ID nr. 4455
Ricchi° M, Cammi° G, Garbarino° C, Buzzini P, Belletti° G, Arrigoni° N
A rapid Real Time-PCR/DNA Melting Resolution Method to identify Prototheca species
J Appl Microbiol. - Vol. 110 ( 2010). - p 27-34. - 33 bib ref
Impact factor: 2,098
First author Ricchi, Matteo
Aim: The study describes the development of a simple and rapid tool to identify yeast-like microalgae
belonging to the genus Prototheca. Methods and Results: The method, based on two-step Real Time PCR
reaction followed by DNA Resolution Melting Analysis (qPCR / RMA), has been developed using reference
strains belonging to both pathogenic (P. zopfii genotype 2, P. wickerhamii and P. blaschkeae) and nonpathogenic species (P. zopfii genotype 1, P. stagnora and P. ulmea). In order to validate the method, seventy
recently isolated Prototheca strains were thus tested in parallel with both the first qPCR /RMA and the
conventional genotype-specific PCR assay: they were classified as P. zopfii genotype 1, P. zopfii genotype 2
and P. blaschkeae, with a perfect accordance between the two above methodologies. Furthermore, we used
the second qPCR /RMA to identify the other species (P. stagnora, P. ulmea and P. wickerhamii), which cannot
be discriminated by conventional PCR assay. Conclusions: The assay two-step Real Time PCR is accurate,
robust, costeffective and faster than auxonographical, biochemical or conventional molecular biology
methods. Significance and Impact of the Study: the rapid and high throughout two-step qPCR /RMA tool can
be usefully used for the identification of clinical and environmental Prototheca species into the framework of
the diagnosis of animal (e.g. bovine mastitis) or human protothecosis.
Pag. 248 di 300 31/08/2011 ID nr. 4456
Petracci M, Amadori° M, Archetti° IL, Bianchi M, Montella L, Cavani C
Effect of Feeding During Long Transport on Body Condition and Physiological Status of Pullets
European Poultry Conference 2010 / [s.n. : s.l., 2010]. - 11 p . - 8 bib ref
European Poultry Conference (13th : Tours, France : August 23-27, 2010)
A study was conducted to test the effects of a newly-developed jelly-like transport feed (TF) on body condition
and physiological status of egg-laying strain pullets transported for more than 12 hours. Four transport trials
were conducted using 440 animals (Lohman Brown, 16-wk-old) loaded in 48×54×21 cm (length×width×height)
metal wire transport crates. Control pullets (C group) were kept without feed and water and loaded at standard
stocking density (8 pullets/crate; 324 sqcm/animal), whereas the experimental group (TF group) had free
access to TF and were loaded by using the same density of C group. Birds were placed on commercial lorries
and transported for 20 hours in autumn, winter and summer corresponding to different environmental
conditions (mild, cold and warm). TF consumption, changes in body weight and body (cloacal) temperature
were assessed. Before catching and after transport, blood samples (15 animals/group) were taken from vena
ulnaris superficialis and used to determine haematocrit, total protein, sodium, glucose, plasma corticosterone,
reactive oxygen metabolites (ROMs), total antioxidant power (OXY-TA) and lysozyme. During the transport,
average TF consumption was 69.7 g/animal (13.0 g dry matter) with an metabolizable energy supply of 0.215
MJ/animal. TF group had lower body weight losses (7.0 vs. 5.9%; P<0.01) and lower decrease of body
temperature. Moreover, TF pullets showed significantly more favourable haematocrit, total protein, sodium,
glucose, plasma corticosterone, ROMs and OXY-TA values. Overall, these findings indicate that TF can be a
useful way to maintain satisfactory body condition and physiological status of pullets during long-distance
journeys.
ID nr. 4457
Razzuoli° E, Olzi E, Calà P, Cafazzo S, Magnani D, Archetti° IL, Nanni_Costa L, Amadori°
M
Clinical immunology parameters in cattle submitted to shortjourneys to the ANAFI Genetic Centre
Buiatria. - Vol. 5 no 2 ( 2010). - p 23-29. - 5 bib ref
Project PRC2007003 - Evaluation of transportation stress with respect to climatic conditions. - Amadori Dr.
Massimo - Capofila - MINSAN - 2007 - 260000,00 EUR - 01/10/2008 0.00.00 - 31/03/2010
0.00.00 - 1 - S1 - Centro Benessere Animale e Immunoprofilassi - Amadori Dr. Massimo - I
2 - Università di Bologna - Dipartimento Protezione e Valorizzazione Agroalimentare - Leonardo
DR: Nanni Costa - E
3 - Università degli Studi di Padova - Daniele dr. Bernardini - E
4 - Università degli Studi della Tuscia - Dipartimento di produzioni Animali - Nicola Prof. Lacetera E
First author Razzuoli, Elisabetta
Transport is a very important stress factor for farro animals; hence, the European and Italiar legislations have
extensively considered the issue. However, journeys of short duration « 8 hours Reg. 1/2005) have been
substantially neglected in that a relevant risk factor is not perceived. Owing tc the above, this study aimed at
evaluating the effects of shortjourneys on the homeostatic adaptatior of Frisian bulls. Our findings indicate a
global adaptation strategy of cattle to various environmenta stressors, of which transportation was not always
of major importance. In such a strategy, the negative acute phase response should be highlighted; this can be
demonstrated 15 days after transportation anc it is sided by an inflammatory cytokine response and a
stepwise increase of serum gamma-globulin this latter result can be probably traced back to the
environmental infectious pressure experienced after transportation.
Pag. 249 di 300 31/08/2011 ID nr. 4458
Gentile A, Bettini G, Dalla_Pria A, Rosignoli° C, Sabattini S, Scarpa F, Testoni S
Clinical and anatomo pathological experiences on the Bovine Neonatal Pancytopenia
Buiatria. - Vol. 5 no 2 ( 2010). - p 3-6. - 6 bib ref
In recent times; similar to other European countries, Italian farmers have also been reporting the worrisome
occurrence of hemorrhagic syndromes associated with pancytopenia in young calves. In this preliminary note
the authors illustrate the clinical and anatomo- pathological aspects of the caseload directly experienced.
ID nr. 4465
Pozzi SP, Alborali° G, Cordioli° P, Rosner A
Investigation of swine influenza sub-types H1N1, H3N2, H1N2 in pigs population in Israel (2002-2009)
Isr J Vet Med. - Vol. 65 no 1 ( 2010). - p 11-14. - 28 bib ref
Impact factor: 0,086
The recent emergence of H1N1 influenza in humans generated concerns about cross-species infections
between hurnans and swine, and the potential amplifing role of infected and densely populated pigs units. In
Israel there are 24 swine breeding units, mainly localized in Northern Region and a single unit in the South;
these units produce about 200,000 slaughtered pigs per year. While in the part, data were collected and
examined for varíous swine respiratory pathogens, no data has been examined for the presence of influenza
virus in swine populations in Israel. This work retrospectively examines and summarizes the epArniological
data for influenza viruses subtypes HIN 1; H3N2, H1N2 in Israel swine population from 2002 to the present
and describes the methods used for serological and virological determinafions. 306 blood samples and 40
organs samples from 31 samplings out of 1.6 swine units vere found to be negative to sub types 1-IINI; H3N2;
H1N2 influenza virus. The consistency of samplings used allowed us to conclude that swine populations in
Israel are negative to sub- type H1N1, H3N2, H1N2 influenza virus. Particular susceptibìlity of naive swine
populations to íntluenza viruses suggests that vaccination of workers involved in the pig industry is indicated
in order to decrease the risk of cross-infection and the possibility of reassortant straín development.
ID nr. 4466
Stercoli° L, Pezzoni° G, Brocchi° E
Evaluation of ORF2 and ORF3 recombiat antigens in HEV antibody-detection ELISA
1st Congress of the European Association of Veterinary Laboratory Diagnosticians (EAVLD) : 15-17
September 2010 Lelystad, the Netherlands : abstracts / [s.l : s.n, c2010]. - O-1-06
Congress of the European Association of Veterinary Laboratory Diagnosticians (EAVLD) (1st : Lelystad,
the Netherlands : 15-17 September 2010)
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Stercoli, Lidia
ID nr. 4467
Pag. 250 di 300 31/08/2011 Benedetti° D, Pezzoni° G, Grazioli° S, Barbieri° I, Brocchi° E
Comparative performance of three genome amplification assays for detection of Swine Vesicular
Disease virus in experimental and field samples
1st Congress of the European Association of Veterinary Laboratory Diagnosticians (EAVLD) : 15-17
September 2010 Lelystad, The Netherlands : abstracts / [s.l : s.n, c2010]. - p. O-2-09. - 2 bib ref
Congress of the European Association of Veterinary Laboratory Diagnosticians (EAVLD) (1st : Lelystad, the
Netherlands : 15-17 September 2010)
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
PRC2008002 - Development and evaluation of Real Time PCR and isothermal amplification
methods for the virological diagnosis of swine vesicular disease - Pezzoni dr.ssa giulia - Solo MINSAN - 2008 - 01/10/2009 0.00.00 - 30/09/2011 0.00.00 - 95000,00 EUR 1 - H1 - Reparto di
Biotecnologie - Pezzoni dr.ssa Giulia - I
First author Benedetti, Dennis
ID nr. 4468
Lelli° D, Moreno° A, Brocchi° E, Sozzi° E, Gamba° D, Autorino G, Clavero J, Angel M,
Cordioli° P
West Nile Virus: characterization of monoclonal antibodies and potential application in laboratory
diagnosis
4th annual meeting Epizone : "Bridges to the future" : 7-10 June, 2010 Saint-Malo, France : abstracts oral
and poster presentations / [s.l. : s. n., 2010]. - p 161-162
Annual meeting Epizone (4th : Saint-Malo, France : 7-10 June, 2010)
Project PRF2005301 - Development of diagnostic tests for surveillance of the equine viral neuropathologies
- Amaddeo Dr. Demetrio - UO - MINSAN - 2005 - 440000,00 EUR - 15/07/2006 0.00.00 14/07/2009 0.00.00 - 1 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo
-I
First author Lelli, Davide
ID nr. 4469
Pezzoni° G, Grazioli° S, Tironi° D, Barbieri° I, Brocchi° E
Validation of a loop-mediated isothermal amplification assay for detection of swine vesicular disease
virus in experimental and field samples
4th annual meeting Epizone : "Bridges to the future" : 7-10 June, 2010 Saint-Malo, France : Abstracts oral
and poster presentations / [s.l. : s. n., 2010]. - p 187
Annual meeting Epizone (4th : Saint-Malo, France : 7-10 June, 2010)
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
Pag. 251 di 300 31/08/2011 PRC2008002 - Development and evaluation of Real Time PCR and isothermal amplification
methods for the virological diagnosis of swine vesicular disease - Pezzoni dr.ssa giulia - Solo MINSAN - 2008 - 01/10/2009 0.00.00 - 30/09/2011 0.00.00 - 95000,00 EUR 1 - H1 - Reparto di
Biotecnologie - Pezzoni dr.ssa Giulia - I
First author Pezzoni, Giulia
ID nr. 4470
Bellini° S, Grazioli° S, Nassuato° C, Lombardi° G, Martinelli° N, Bugnetti° M, Eblè P,
Dekker A
An experimental infection with swine vesicular disease virus in pregnant sows to determine the
duration of passive immunity in piglets
4th annual meeting Epizone : "Bridges to the future" : 7-10 June, 2010 Saint-Malo, France : abstracts oral
and poster presentations / [s.l. : s. n., c2010]. - p 178
Annual meeting Epizone (4th : Saint-Malo, France : 7-10 June, 2010)
Project epizone - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Bellini, Silvia
ID nr. 4471
Delogu M, De_Marco MA, Di_Trani L, Raffini° E, Cotti C, Puzelli S, Ostanello F, Webster
RG, Cassone A, Donatelli I
Can Preening Contribute to Influenza A Virus Infection in Wild Waterbirds?
PLoS One. - Vol. 5 no 6 ( 2010). - p e11315. - 60 bib ref
Impact factor: 4,351
Project PRC2002003 - Experimental transmission of Italian BSE strain to cattle: clinical observation, strain
characterization, in vivo PrPres and molecular marker detection; establishment of reference samples
for Nation-wide research. - Capucci Dr. Lorenzo - Capofila - MINSAN - 2002 - 306402,10 EUR 17/11/2003 0.00.00 - 31/08/2006 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Capucci Dr.
Lorenzo - I
2 - IZSPLV - Caramelli Dr.ssa Maria - E
3 - IST. NEUROLOGICO BESTA - Tagliavini Dr. Fabrizio - E
4 - UNI VR - Monaco Dr. Salvatore - E
Wild aquatic birds in the Orders Anseriformes and Charadriiformes are the main reservoir hosts perpetuating
the genetic pool of all influenza A viruses, including pandemic viruses. High viral loads in feces of infected
birds permit a fecal-oral route of transmission. Numerous studies have reported the isolation of avian
influenza viruses (AIVs) from surface water at aquatic bird habitats. These isolations indicate aquatic
environments have an important role in the transmission of AIV among wild aquatic birds. However, the
progressive dilution of infectious feces in water could decrease the likelihood of virus/host interactions. To
evaluate whether alternate mechanisms facilitate AIV transmission in aquatic bird populations, we
investigated whether the preen oil gland secretions by which all aquatic birds make their feathers waterproof
could support a natural mechanism that concentrates AIVs from water onto birds' bodies, thus, representing a
possible source of infection by preening activity. We consistently detected both viral RNA and infectious AIVs
on swabs of preened feathers of 345 wild mallards by using reverse transcription–polymerase chain reaction
(RT-PCR) and virus-isolation (VI) assays. Additionally, in two laboratory experiments using a quantitative realtime (qR) RT-PCR assay, we demonstrated that feather samples (n = 5) and cotton swabs (n = 24)
experimentally impregnated with preen oil, when soaked in AIV-contaminated waters, attracted and
concentrated AIVs on their surfaces. The data presented herein provide information that expands our
understanding of AIV ecology in the wild bird reservoir system.
Pag. 252 di 300 31/08/2011 ID nr. 4472
Galarini R, Fioroni L, Moretti S, Dusi° G
Development and validation of a confirmatory method for eleven coccidiostats in eggs
6th Intenational Symposium on Hormone and Veterinary Drug Residue Analysis : Ghent, Belgium, June 1-4,
2010 : abstract book / [s.l. : s.n., 2010]. - p 92
Intenational Symposium on Hormone and Veterinary Drug Residue Analysis (6th. : Ghent, Belgium : June
1-4, 2010)
First author Dusi, Guglielmo
A confirmatory method for the determination of residues for eleven coccidiostats including ionophore
antibiotics (lasalocid, maduramycin, monensin, narasin, salinomycin, semduramycin) and chemical
coccidiostats (decoquinate, diclazuril, halofuginone, nicarbazin and robenidine) in poultry eggs was
developed. The analytes were chosen considering the comprehensive list of authorised coccidiostats reported
in the Community Register of Feed Additives as established by Regulation (EC) 1831/2003. The sample
preparation was based on extraction with acetonitrile, defatting with hexane and clean-up on Silica SPE. The
target compounds were identified (at least four identification points) and quantified by high performance liquid
chromatography (HPLC) coupled with electrospray ionisation tandem mass spectrometry (ESI/MS/MS) setting
positive or negative ionisation mode. The method performance characteristics required by Commission
Decision 2002/657/EC were estimated adopting the more flexible and simple validation design suggested by
Kaufmann (Anal. Chim. Acta, 637 (2009) 144). This alternative approach, instead of the fixed Decision plan
which involves a narrow validation interval (0.5-1.5 times the permitted limit), focus the experimental study on
a larger dynamic range (two orders of magnitude), allowing the simultaneous validation of several substances
with significantly different legal limits.
ID nr. 4473
Dusi° G, Bozzoni° E, Tognoli° N, Assini° W, Gasparini° M, Ferretti° E
Development and validation of a confirmatory method for the determination of nine corticosteroids in
liver sample by liquid chromatography-tandem mass spectrometry
6th Intenational Symposium on Hormone and Veterinary Drug Residue Analysis : Ghent, Belgium, June 1-4,
2010 : abstract book / [s.l. : s.n., 2010]. - p 95
Intenational Symposium on Hormone and Veterinary Drug Residue Analysis (6th. : Ghent, Belgium : June
1-4, 2010)
First author Dusi, Guglielmo
Corticosteroids belong to the class of the steroid hormones and they are important in normal protein,
carbohydrate and fat metabolism and for their role in controlling inflammation. Besides naturally occurring
corticosteroids, which are secreted by the adrenal cortex, a whole range of chemical synthetic analogs (e.g.
dexamethasone and prednisolone) have been developed. In the EU legislation the use of synthetic
corticosteroids as growth promoters in livestock breeding is prohibited. However, a few synthetic
corticosteroids have a legal use and, for this reason, maximum residue levels (MRL) for these drugs are
established in matrices as bovine liver. Several analytical procedures, with a deconjugation step, for the LC–
MS/MS analysis of corticosteroids in liver sample have been published in recent years, but they are complex
and time consuming. The aim of this work is the development of a reliable but simply and fast sample
deconjugation/purification method for the identification and quantification of nine synthetic corticosteroids. The
liver sample was subjected to an enzymatic deconjugation and then to a one-step cleanup on an Waters
OASIS HLB SPE cartridge. The analytes were detected by liquid chromatography-negative-ion electrospray
tandem mass spectrometry using deuterium-labelled internal standards. The method was validated as a
quantitative confirmatory method according to European Commission Decision 2002/657/CE. The evaluated
parameters were: linearity, specificity, precision (repeatability and within-laboratory reproducibility), recovery,
decision limit, detection capability and ruggedness. All the analytes should be detected above one µg kg-1
with a recovery above 60 %. Repeatability was below 8 % and within-laboratory reproducibility below 16 % for
the nine corticosteroids.
Pag. 253 di 300 31/08/2011 ID nr. 4474
Gradassi° M, Zanoni° M, Salogni° C, Tagliabue° S, D'Incau° M, Bertasi° B, Losio° MN,
Boni° P, Alborali° GL
Phagetyping, ribopyting and antimicrobial resistance profiles of Salmonella Typhimurium isolated
from Italian heavy pigs at slaughter
International Symposium Salmonella and Salmonellosis : June 28-30, 2010 Saint-Malo, France :
Proceedings / [s.l. : s.n., 2010]. - p 275-276
International Symposium Salmonella and Salmonellosis : Saint-Malo, France : June, 28-30, 2010)
Project PRC2007007 - An investigation into the main enteric disease agents of swine with potential zoonotic
repercussions. - Tagliabue Dr.ssa Silvia - Solo - MINSAN - 2007 - 80000,00 EUR - 03/11/2008
0.00.00 - 02/05/2011 0.00.00 - 1 - U2 - REPARTO DI BATTERIOLOGIA - Tagliabue Dr.ssa Silvia
-I
2 - M6 - Sezione diagnostica provinciale di Mantova - Nigrelli Dr. Arrigo - I
3 - N6 - Sezione diagnostica provinciale di Piacenza - Belletti Dr. Gian Luca - I
First author Gradassi, Matteo
ID nr. 4475
Bonardi S, Paris A, Bassi L, Salmi F, Bacci C, Riboldi E, Boni E, D'Incau° M, Tagliabue° S,
Brindani F
Detection, semiquantitative enumeration, and antimicrobial susceptibility of Yersinia enterocolitica
in pork and chicken meats in Italy
J Food Prot. - Vol. 73 no 10 ( 2010). - p 1785-1792. - 71 bib ref
Impact factor: 1,960
Yersinia enterocolitica is recognized as an etiological agent of gastroenteritis, lymphadenitis, and chronic
sequelae. During 2006 and 2007, 205 samples (125 pork and 80 chicken meats) were collected in Italy and
tested for detection and most-probable-number (MPN) enumeration of Y. enterocolitica organisms. The
microorganism was isolated from 45 samples (21.9%): 19 (15.2%) pork samples and 26 (32.5%) chicken
samples. Y. enterocolitica MPN contamination levels were low, ranging from 0.30 to 1.50/g. Most (94.4%) Y.
enterocolitica strains were biotype 1A (serotypes O:3; O:5; O:6,30; O:6,30-6,31; O:7,8-8-8,19; O:8; O:9;
O:25,35; O:36; and O nontypeable), and 5.6% of the isolates were bioserotype 2/O:9. All isolates were tested
for yadA, ail, inv, ystA, and ystB virulence sequences. The yadA gene was detected in two strains (3.7%)
isolated from chicken samples: one Y. enterocolitica 2/O:9 yadA+ ail+ ystA+, and one Y. enterocolitica
1A/O:7,8-8-8,19 yadA+ inv+ ystB+. Two (3.7%) 2/O:9 strains, isolated from pork products, were ail+ ystA+.
Most biotype 1A strains were ystB+ (84.3%) and inv+ (39.2%). All strains were sensitive to cefotaxime,
ciprofloxacin, chloramphenicol, nalidixic acid, streptomycin, sulfonamide, tetracycline, trimethoprim, and
trimethoprim-sulfamethoxazole. Resistance to gentamicin and aztreonam was observed in 1.9% of the
isolates. High levels of resistance were detected toward amoxicillin-clavulanic acid (27.8%), ampicillin
(75.9%), and erythromycin (100%). The authors hypothesize that Y. enterocolitica pathogenic biotypes are
rather uncommon in foods when compared with their isolation rates from animal sources and that chicken
meat could be contaminated as well as pig meat and its derived products.
ID nr. 4476
Gelmetti° D, Bertoletti° I, Giudice C
Bilateral complex microphthalmia with intraocular dermoid cyst in a neonate red deer (Cervus
elaphus)
J Wild Dis. - Vol. 46 no 3 ( 2010). - p 961-965. - 16 bib ref
Impact factor: 1,373
First author Gelmetti, Daniela
Pag. 254 di 300 31/08/2011 A 3-day-old, male red deer (Cervus elaphus) with bilateral microphthalmia was found dead in the Western
Alps in northern Italy. No other gross alterations were present. Ocular globes were formalin fixed and
processed for histology. In both eyes, a large cyst, filled with keratin and lined by squamous epithelium
admixed with sebaceous glands and hair follicles, expanded the anterior chamber. A mass composed of
spindle cells and small tubules, embedded in myxoid ground substance, filled the vitreal chamber. No lens
tissue was found, leading to the speculation that the dermoid cystic structures originated from abnormal
differentiation of the primitive ectodermal lens vesicle. Immunohistochemistry with a panel of antibodies
against cytoskeletal proteins revealed neural and mesenchymal elements within the vitreal mass. A complex
bilateral ocular dysgenesis, with microphthalmia, aphakia, and dermoid metaplasia of primitive lens vesicle
was diagnosed. The latter finding was unusual and was previously documented only in Texel sheep (Ovis
ammon), in which it was related to specific gene mutation on chromosome 23..
ID nr. 4477
Martella V, Bianchi° A, Bertoletti° I, Pedrotti L, Gugiatti A, Catella° A, Cordioli° P, Lucente
MS, Elia G, Buonavoglia C
Canine distemper epizootic among Red Foxes, Italy, 2009
Emerg Inf Dis. - Vol. 16 no 12 ( 2010). - p 2007-2009. - 10 bib ref
Impact factor: 6,794
ID nr. 4478
Cavadini° P, Botti° G, Barbieri° I, Lavazza° A, Capucci° L
Molecular characterization of SG33 and Borghi vaccines used against myxomatosis
1st Congress European Association of Veterinary Laboratory Diagnosticians (EAVLD) : 15-17 September,
2010 Lelystad, The Netherlands : abstract / [s.l. : s.n., 2010]. - P 2-22. - 4 bib ref
Congress European Association of Veterinary Laboratory Diagnosticians (EAVLD) (1st : Lelystad, The
Netherlands : 15-17 September, 2010)
Project PRC2003020 - Development of advanced laboratory methods for diagnosis and typing of rabbit
mixoma virus - Capucci Dr. Lorenzo - Capofila - MINSAN - 2003 - 90000,00 EUR - 26/04/2004
0.00.00 - 25/10/2006 0.00.00 - 1 - H2 - Reparto di Biologia Molecolare - Capucci Dr. Lorenzo - I
2 - G1 - Reparto di Virologia e Sierologia Specializzata - Lavazza Dr. Antonio - I
3 - IZSVE - Agnoletti Dr. Fabrizio - E
PRC2008003 - Development of molecular methods (or approaches) to characterize the DNA of
myxoma virus field isolates - Cavadini dr.ssa Patrizia - Solo - MINSAN - 2008 - 01/09/2009
0.00.00 - 31/08/2011 0.00.00 - 58000,00 EUR 1 - H2 - Reparto di Biologia Molecolare - Cavadini
dr.ssa Patrizia - I
First author Cavadini, Patrizia
Myxoma virus is a poxvirus responsible for myxomatosis in European Rabbits (Oryctolagus cuniculus). The
entire genome of the myxoma virus has been sequenced, allowing a systemic survey of the functions of a
large number of putative pathogenic factors that this virus expresses to subvert the immune and inflammatory
pathways of infected rabbit hosts. In Italy, industrial rabbits are mostly vaccinated against myxomatosis using
the attenuated myxoma virus strains Borghi or SG33. We have identified genetic markers specific for Borghi
or SG33 vaccine strains and established a PCR-based assay that could be used to: (a) rapidly diagnose the
presence of myxoma virus in infected organs; (b) discriminate between field strain-infected and vaccinated
rabbits and (c) differentiate between Borghi or SG33 vaccine strain.
ID nr. 4479
Calzolari° M, Bonilauri° P, Bellini R, Caimi M, Defilippo° F, Maioli° G, Albieri A, Medici A,
Veronesi R, Pilani R, Gelati A, Angelini P, Parco V, Fabbi° M, Barbieri° I, Lelli° D,
Pag. 255 di 300 31/08/2011 Lavazza° A, Cordioli° P, Dottori° M
Arboviral survey of mosquitoes in two Northern italian regions in 2007 and 2008
Vector Borne Zoonotic Dis. - Vol. 10 no 9 ( 2010). - p 875-884. - 47 bib ref
Impact factor: 2,607
First author Calzolari, Mattia
Recently, Italy - particularly the Emilia-Romagna region - was the location of consecutive outbreaks of human
diseases caused by the arboviruses chikungunya virus and West Nile virus. The two outbreaks, spread by
different species of mosquitoes, were not related, but pointed out the lack of an arboviral surveillance program
in this region. Beginning in 2007 entomological surveillance was initiated in the Emilia-Romagna region, and
in 2008 the program was improved and extended at Lombardia region. Using CO2-baited traps, 65,292
mosquitoes were collected; pooled by date of collection, location, and species; macerated manually; and
tested by reverse transcription (RT)-polymerase chain reaction for the presence of alphaviruses,
orthobunyaviruses, and flaviviruses. Amplicons were sequenced and employed for identification of viral RNA
by basic local alignment search tool search in GenBank. Results of these assays showed (1) the presence of
West Nile virus in two pools of Culex pipiens mosquitoes, (2) the presence of RNA of two orthobunyaviruses,
Tahyna virus in a pool of Ochlerotatus caspius mosquitoes and Batai virus in a pool of Anopheles
maculipennis mosquitoes, and (3) the presence of flavivirus RNAs in pools of Oc. caspius, Aedes albopictus,
and Aedes vexans mosquitoes; the sequences of these amplicons were most closely related to flaviviruses
that have been detected only in mosquitoes and had no recognized vertebrate host (Aedes flavivirus, Culex
flavivirus, and Kamiti River virus).
ID nr. 4480
Pajoro° M, Pistone D, Epis S, Sassera D, Montagna M, Luzzago C, Lanfranchi P, Viganò
R, Cerutti M, Vicari° N, Fabbi° M, Bandi C, Genchi C
A survey for bacterial pathogens associated with Ixodes ricinus in north-western Italy
Parassitologia. - Vol. 52 no 1-2 ( 2010). - p 178
Congresso Nazionale della Societa' Italiana di Parassitologia (26. : Perugia : 22-25 giugno 2010)
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
First author Pajoro, Massimo
ID nr. 4481
Benedetti° V, Cremonesi P, Ferrari° S, Castiglioni B, Fabbi° M, Vicari° N, Garbarino° C,
Battisti A, Franco A, Feltrin F, Luini° M
Methicillin-resistant Staphylococcus aureus (MRSA) from bovine milk samples
Large Anim Rev. - Vol. 16 ( 2010). - p 67-70. - 22 ref bib
Project PRC2008008 - Staphylococcus aureus: methicillin-resistance and molecular characterization of the
strains isolated from pets and other diagnostic sources - Luini Dr. Mario - Capofila - MINSAN 2008 - 60000,00 EUR - 01/09/2009 0.00.00 - 31/08/2011 0.00.00 - 1 - M5 - Sezione diagnostica
provinciale di Lodi - Luini Dr. Mario - I
2 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
3 - Istituto Zooprofilattico Lazio e Toscana - Battisti Dr. Antonio - E
First author Benedetti, V
Pag. 256 di 300 31/08/2011 Aim - Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important hospital and
community-acquired pathogen. MRSA are also reported in farm animal and more recently reported as cause
of bovine mastitis in Belgium and Germany. The aim of this study was to asses tbc presence of MRSA in
some herds of tbc Po Valley and provi-de further characterization of tbc isolates. Results - Between October
2008 and February 2010, 2875 strains of S. aureus were isolateti from different herds. A sample of 425
strains, representative of different farrns of origin, were tested for methicillin-resistance by oxacillin agar
diffusion test and nrecA gene PCR. 55 MRSA strains were isolated from 20 different dairy farms. Fourteen
MRSA randomly selected, were genotyped for the presence of tbc main recognized virulence factors and
characterized by spa-typing, MLST and SCCmec typing. The characterization of tbc MRSA strains showed
higher frequency of ST398, t899 spa-typing, tbc typical profile of livestock-associated MRSA (LA-MRSA). A
strain was belonging to genotype t355, ST152, associated with human infections. Four MRSA were
enterotoxigenic. Conclusions - MRSA are present in several cattle herds in tbc Po Valley and may represent a
reservoir of infection for humans, in particular for those involved in animal husbandry, and a foodborne risk
through the contamination of the food chain. Management of contagious MRSA mastitis should consider strict
contro] measures and eradication among effective options.
ID nr. 4482
Canelli° E, Luppi° A, Lavazza° A, Lelli° D, Sozzi° E, Moreno° A, Gelmetti° D, Pascotto E,
Sandri C, Magnone W, Cordioli° P
Encephalomyocarditis virus infection in an Italian zoo
Virol J. - Vol. 7 ( 2010). - p 64. - 21 bib ref
Impact factor: 2,435
Project PRC2006001 - Development and implementation of epidemiological surveillance and risk analysis
for primary production and processing of typical area food - Boni Dr. Paolo - Solo - MINSAN - 2006
- 1131023,00 EUR - 02/07/2007 0.00.00 - 01/07/2010 0.00.00 - 1 - M6 - Sezione diagnostica
provinciale di Mantova - Nigrelli Dr. Arrigo - I
2 - N1 - Sezione diagnostica provinciale di Bologna - Belletti Dr. Gian Luca - I
3 - I3 - Reparto Microbiologia e Parassitologia degli Alimenti e Sorveglianza Epidemiologica - Boni
Dr. Paolo - I
4 - H1 - Reparto di Biotecnologie - Brocchi Dr.ssa Emiliana - I
5 - G1 - Reparto di Virologia e Sierologia Specializzata - Cordioli Dr. Paolo - I
First author Canelli, Elena
A fatal Encephalomyocarditis virus (EMCV) infection epidemic involving fifteen primates occurred between
October 2006 and February 2007 at the Natura Viva Zoo. This large open-field zoo park located near Lake
Garda in Northern Italy hosts one thousand animals belonging to one hundred and fifty different species,
including various lemur species. This lemur collection is the most relevant and rich in Italy. A second outbreak
between September and November 2008 involved three lemurs. In all cases, the clinical signs were sudden
deaths generally without any evident symptoms or only with mild unspecific clinical signs. Gross pathologic
changes were characterized by myocarditis (diffuse or focal pallor of the myocardium), pulmonary congestion,
emphysema, oedema and thoracic fluid. The EMCV was isolated and recognized as the causative agent of
both outbreaks. The first outbreak in particular was associated with a rodent plague, confirming that rats are
an important risk factor for the occurrence of the EMCV infection.
ID nr. 4488
Mutze G, Kovaliski J, Butler K, Capucci° L, McPhee S
The effect of rabbit population control programmes on the impact of rabbit haemorrhagic disease in
south-eastern Australia
J Appl Ecology. - Vol. 47 ( 2010). - p 1137-1146. - 30 bib ref
Impact factor: 4,197
Pag. 257 di 300 31/08/2011 The effect of rabbit population density on transmission of rabbit haemorrhagic disease virus (RHDV) is a
critical aspect of disease ecology for rabbit control and rabbit conservation. We examined the interaction
between rabbit control and spread of RHDV and a non-pathogenic calicivirus (bCV) in Australian wild rabbit
populations, and reviewed existing recommendations for control in this context. Rabbits were sampled at eight
pairs of sites; from rabbit populations where densities had been reduced by conventional control and from
matching uncontrolled populations. Sites chosen ranged from hot, arid areas where RHDV had greatly
reduced rabbit numbers to cooler, higher-rainfall areas where rabbits remained more abundant. Virus activity
was implied from antibody profiles in sera of surviving rabbits. Reducing population density by conventional
control had a similar effect on disease transmission despite a seven-fold difference in initial density.
Populations reduced by 70% or more had lower RHDV antibody prevalence in juvenile rabbits but not in adult
rabbits, indicating that reducing rabbit density slowed but did not stop RHDV transmission. We found no
interactions between rabbit control, RHDV and bCV that could be exploited to improve rabbit management.
Synthesis and applications. Delayed RHDV infection in rabbit control sites is likely to be offset by higher
mortality in older rabbits, so that conventional rabbit control does not reduce the impact of RHDV on rabbit
populations. Only minor changes to delay the timing of summer rabbit control programmes in cooler areas of
Australia are necessary to take best advantage of RHDV-induced reduction in rabbit numbers. For
conservation management of rabbits in Europe, these findings indicate that RHDV may continue to have a
severe impact on rabbit populations that have been reduced to low population density, but also raise the
possibility that bCVs might be introduced to rabbit populations to aid their recovery.
ID nr. 4489
Mutze G, Sinclair R, Peacock D, Kovaliski J, Capucci° L
Does a benign calicivirus reduce the effectiveness of rabbit haemorrhagic disease virus (RHDV) in
Australia? Experimental evidence from field releases of RHDV on bait
Wildl Res. - Vol. 37 no 4 ( 2010). - p 311-319. - 30 bib ref
Impact factor: 1,222
Context. European rabbits are serious environmental and agricultural pests throughout their range in
Australia. Rabbit haemorrhagic disease virus (RHDV) greatly reduced rabbit numbers in arid central Australia
but had less impact in cooler, higher-rainfall areas. RHDV-like benign caliciviruses (bCVs) have been
implicated in limiting the impact of RHDV in the higher-rainfall regions of Australia and also in Europe Aims.
Experimental releases of RHDV on bait were tested as a means of initiating disease outbreaks. Serological
evidence of antibodies to bCVs was examined to determine whether they reduce mortality rates and/or spread
of the released RHDV, and how that might influence the effectiveness of future RHDV releases for rabbit
management. Methods. Four experimental releases were conducted in high-rainfall and coastal regions of
southern Australia. Virus activity was implied from recapture rates and serological changes in marked rabbits,
and genetic sequencing of virus recovered from dead rabbits. Changes in rabbit abundance were estimated
from spotlight transect counts. Key results. Release of RHDV on bait produced disease outbreaks that
challenged almost all animals within the general release area and spread up to 4 km beyond the release sites.
Recapture rates were high in marked rabbits that possessed antibodies from previous exposure to RHDV and
extremely low amongst rabbits that lacked any detectable antibodies. Rabbits carrying antibodies classified as
being due to previous infection with bCVs had recapture rates that were dependent on circulating antibody
titre and were ~55% of recapture rates in rabbits with clear antibodies to RHDV. Conclusions. This is the first
quantified evidence that antibodies produced against bCVs provide significant protection against RHD
outbreaks in field populations of rabbits. Implications. bCVs can greatly reduce the impact of RHDV on wildrabbit populations in Australia and presumably elsewhere. RHDV can be effectively released on bait although
further releases are likely to be of minor or inconsistent benefit for controlling rabbit numbers where bCVs are
common.
ID nr. 4528
Mariella J, Pirrone A, Antenucci E, Archetti° I, Castagnetti C
Haematological and biochemical parameters of periparturient mares
Pag. 258 di 300 31/08/2011 VIII Congresso Nazionale Societa' Italiana Diagnostica di Riproduzione Animale (SIRA) : 17-18 Giugno 2010
Ozzano dell'emilia (BO) / [s.l. : Societa' Italiana Diagnostica di Riproduzione Animale (SIRA), 2010]. - p 87. 3 bib ref
Congresso Nazionale Societa' Italiana Diagnostica di Riproduzione Animale (SIRA) (8. : Ozzano dell'emilia
(BO) : 17-18 Giugno 2010)
ID nr. 4529
Tosi° G, Taddei° R, Barbieri I, Fiorentini° L, Massi° P
Caratterizzazione molecolare dei ceppi di virus della bronchite infettiva aviare isolati in Italia nel
periodo 2007-2009 e nel primo bimestre del 2010
XLIX Convegno annuale Societa' Italiana di Patologia Aviare (SIPA) : 29-30 Aprile 2010 Forlì : atti / Parma :
La Ducale, 2010. - p 217-224. - 8 ref bib
Convegno annuale Societa' Italiana Patologia Aviare (SIPA) (49 : Forli' : 29-30 Aprile 2010)
First author Tosi, Giovanni
A molecular survey of IBV strains detected in Italy during the period 2007-2009 (and during the first two
months of 2010) was performed. Serotype 793B is still the most prevalent IBV strain affected the italian
poultry industry. However, an increase of the prevalence of QX strain and IT-02 strain was observed,
especially during the beginning of this year. In addition, other IBV strains (D274 and B1648) reappeared in
Italy.
ID nr. 4537
Moreno° A, Fiorentini° L, Taddei° R, Barbieri° I, Tosi° G, Cordioli° P, Massi° P
Pandemic H1N1 virus outbreak in a cat colony in Italy
4th annual meeting Epizone : "Bridges to the future" : 7-10 June, 2010 Saint-Malo, France : abstracts oral
and poster presentations / [s.l. : s. n., c2010]. - p 179
Annual meeting Epizone (4th : Saint-Malo, France : 7-10 June, 2010)
Project EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Moreno_Martin, Ana
ID nr. 4539
Bertocchi° L, Dusi° G, Vismara° F, Daga° S, Arioli F, Casati A, Pompa G
Preliminary observations of the presence of prednisolone in dairy cattle urine samples
XXVI World Buiatrics Congress : November 14-18, 2010 Santiago, Chile : abstracts / [s.l. : s. n., 2010]. - cdrom
World Buiatrics Congress (26th : Santiago, Chile : November 14-18, 2010)
Project PRC2009010 - Impact of transportation stress on plasma levels of cortisol and cortisol-like
molecules. - Bertocchi Dr. Luigi - Solo - MINSAN - 2009 - 81351,00 EUR - 15/09/2010 0.00.00 14/09/2011 0.00.00 - 1 - V6 - REPARTO PRODUZIONE PRIMARIA - Bertocchi Dr. Luigi - I
2 - V1 - REPARTO CHIMICA DEGLI ALIMENTI DI ORIGINE ANIMALE - Dusi Dr. Guglielmo - I
3 - NA - Reparto Merceologia degli Alimenti di origine animale (Bologna) - Fedrizzi Dr. Giorgio - I
4 - K1 - REPARTO ANIMALI DA LABORATORIO - Archetti Dr.ssa Ivonne - I
First author Bertocchi, Luigi
Objectives: In the years 2008-09 the Lombardy region has supported a research with the purpose to detect
the presence of drug residues in urine and liver samples collected from cattle at the slaughterhouse. All liver
Pag. 259 di 300 31/08/2011 samples resulted to be negative, while the 72% of samples of urine were positive for prednisolone presence.
Due to the high number of positive samples it was hypothesized that the stress transport and pre-slaughter
conditions could lead to the production of prednisolone in urine. In order to confirm this hypothesis bovine
dairy cattle were treated with adrenocorticotropic hormone (ACTH) to simulate stress condition.. Materials and
methods: Three Holstein Fresian cows (multiparous, non-pregnant, over 200 days lactating with 29 kg/day
mild milk production), were selected. Urine samples were obtained by bladder catheterization before and after
the double intramuscular treatment with 1 ml of Sinacthen (Defiant Pharmaceutical) which corresponds to 200
IU of ACTH. Samples were collected at the start of the investigation, after treatment and the time of slaughter
(tab. 1). Urine analyses to detect predinisolone presence were performed at the Anabolic and drug residue
laboratory of IZSLER with LC-MS/MS system and limit of quantification of 0.58 ng/ml. Results: At the start of
the study, all cows were negative for two samples before treatment. In contrast,, all urine samples collected at
two and six hours after treatment were positive with prednisolone values between 0.69 and 1,48 ppb.
Moreover, with the exception of bovine no. 815 ( sample collected at 42nd hour) no drug was detected in the
samples collected 18 and 42 hours after treatment. All samples after transport and slaughter resulted to be
positive with prednisolone values between 0.63 and 0,82 ppb. Conclusions: The presence of prednisolone
after ACTH treatment seems to suggest the possibility that this molecule could be physiologically produced.
Other studies need to be performed in order to confirm this hypothesis and to clarify the metabolic pathway.
ID nr. 4540
Benedetti° V, Parisi A, Miccolupo A, Castiglioni V, Vezzoli° F, Piccinini R, Scanziani E,
Luini° M
Microbiological and immunohystochemical study of Campylobacter jejuni intramammary infection in
dairy cows
XXVI World Buiatrics Congress : November 14-18, 2010 Santiago, Chile : abstracts / [s.l. : s. n., 2010]. - cdrom
World Buiatrics Congress (26th : Santiago, Chile : November 14-18, 2010)
First author Benedetti, Valentina
Objective: Campylobacter jejuni is a major cause of food-borne disease in developed countries. Poultry
products are considered the most common source of human infection, but unpasteurised cow milk is also a
significant source. C. jejuni is a frequent inhabitant of cattle intestine, while intramammary infection was rarely
reported. In our study, we evaluated microbiological and pathological findings in three cases of C. jejuni
intramammary infection of dairy cows. Methodology: In three dairy farms showing the presence of C. jejuni in
the bulk milk, composite or quarter milk samples were taken. At slaughter, udder, liver, bile and intestinal
tracts were collected from 3 cows. Samples were cultured in Bolton broth and plated after 48h on mCCD and
Skirrow agar after filtration through 0.45 µm membrane. Suspected colonies were Gram stained and identified
with a C. coli / C. jejuni multiplex-PCR. All C. jejuni strains were genotyped using MLST. Tissue samples were
processed for histological evaluation and stained with hematoxylin and eosin and immunohistochemically for
detection of Campylobacter spp. antigen. Results: C. jejuni was isolated from the milk samples of 1 cow from
each of the herds. C. jejuni was also isolated from the mammary tissue and the intestine of these 3 cows
(cases A, B and C). The strains from the 3 farms belonged to different Sequence Types (STs). Moreover, the
strains infecting the mammary gland were different from those identified in the intestine. In two cows, different
STs were also evidenced in different intestinal tracts of the same animal. In the three cases examined,
aspecific findings of mild to moderate neutrophilic MASTITIS, lymphoplasmacytic galactophoritis and
lymphoplasmacytic enteritis were observed. Campylobacter spp. antigen was detectable by
immunohistochemistry only in small intestine of case A and in small and large intestine of case B.
Conclusions: MLST analysis evidenced a high diversity among isolates, both inter- and intra-herds. As
expected, C. jejuni from milk and mammary tissues of each animal belonged to the same ST, but these
mammary strains were different from those found in the intestine. The results of this study suggest that
intramammary infection occur more likely from the environment by intracanalicular route, rather than from
autogenous infection from intestine. Further, the presence of C. jejuni would not related with specific lesions of
the mammary gland.
Pag. 260 di 300 31/08/2011 ID nr. 4541
Martelli F, Toma S, Di_Bartolo I, Caprioli A, Ruggeri FM, Lelli° D, Bonci° M, Ostanello F
Detection of Hepatitis E Virus (HEV) in Italian pigs displaying different pathological lesions
Res Vet Sci. - Vol. 88 ( 2010). - p 492-496. - 39 bib ref
Impact factor: 1,345
In this study we investigated the HEV prevalence in Italian pigs displaying different pathological lesions,
possible risk factors related to the infection, and the possible relations occurring between HEV and other
concomitant pig pathogens. Genetic characterization of some of the identified strains was also performed.
Detection of HEV RNA was accomplished using a nested reverse-transcription polymerase chain reaction on
bile samples from 137 pigs of 2–4 months of age submitted for diagnostic purposes. Forty-one of the 137
examined pigs (29.9%) tested positive for HEV RNA. Animals of 80–120 days of age showed a higher
prevalence of HEV infection (46.9% against 20% of younger animals). No statistically significant correlations
between HEV positivity and the presence of other pathological conditions detected at necropsy, or
concomitant coinfections with PCV2 and/or PRRSV were detected. All identified strains belonged to genotype
3, and were similar to other HEV subtypes 3e, 3f, 3c circulating in Europe.
ID nr. 4542
Sozzi° E, Barbieri° I, Lavazza° A, Lelli° D, Moreno° A, Canelli° E, Bugnetti° M, Cordioli° P
Molecular characterization and phylogenetic analysis of VP1 of Porcine Enteric Picornaviruses
isolates in Italy
Transboundary Emerg Dis. - Vol. 57 ( 2010). - p 434-442. - 23 bib ref
Impact factor: 1,854
First author Sozzi, Enrica
Porcine enterovirus (PEV), Porcine Teschovirus and Porcine sapelovirus, belonging to the family
Picornaviridae, are ubiquitous and mainly cause asymptomatic infections in pigs. In this study, a total of 40
Italian porcine picornavirus isolates were characterized by sequencing the capsid VP1-encoding gene. This
procedure turned out to be a useful diagnostic tool for the molecular identification of porcine enterovirus,
teschovirus and sapelovirus strains and for the study of molecular epidemiology and evolution of these viruses
confirming the possibility of correlating virus genotype to serotype.
ID nr. 4543
Finazzi° G, Daminelli P, Oliverio° E, De_Nadai° V, Monastero° P, Rossi° F, Bertasi° B,
Losio° MN, Boni° P
Dynamics of Listeria monocytogenes, Salmonella typhimurium and Escherichia coli O157:H7 during
the production of 'Nduja
Ind Aliment (Pinerolo). - Vol. 49 n 507 ( 2010). - p 38-42. - 7 bib ref
First author Finazzi, Guido
The aim of this survey was to evaluate Listeria monocytogenes, Salmonella typhimurium and Escherichia coli
O157:H7 fate during production process of 'Nduia, traditional Italian Salami. For this purpose the meat
kneading provided by Salumificio artigianale F.lli Pugliese was contaminated with mixtures of these
microorgonisms, then bagged and seasoned according to the processing of the producer. The results of this
challenge test showed thot the seasoning of the product reduces the concentration of pathogens artificially
added.
Pag. 261 di 300 31/08/2011 ID nr. 4544
Andreoli E, Bertoletti° I, Bianchi° A, Mattiello S
Sanitary survey of wild red deer Cervus elaphus hunted in Sondrio Province (Italy)
Folia Vet. - Vol. 54 no 1 ( 2010). - p 37-41. - 33 bib ref
To investigate the health conditions of the wild red deer (Cervus elaphus) in Sondrio Province (Central Italian
Alps) 43 blood samples, 48 lung samples, 37 pulmonary lymph nodes, 34 fragments of diaphragms, 13
samples of small intestines and 30 faecal samples were collected from 61 red deer culled during the 2006
hunting season (lst September—15th December). This study revealed the presence of respiratory syncytial
virus, Leptospira interrogans serovar grippotyphosa, Mvcobacterium avium complex and gastrointestinal
parasites, and confirmed the absence of Trichinella sp., bovine pestivirus, bovine herpesvirus type 1,
parainfluenza virus type 3, Mrcobacterium bovis and M. avium subsp. para tuberculosrs. More investigations
are required to confirm the presence of Brucella spp..
ID nr. 4545
Ricchi° M, Goretti M, Branda E, Cammi° G, Garbarino° CA, Turchetti B, Moroni P,
Arrigoni° N, Buzzini P
Molecular characterization of Prototheca strains isolated from Italian dairy herds
J Dairy Sci. - Vol. 93 ( 2010). - p 4625-4631. - 31 bib ref
Impact factor: 2,463
First author Ricchi, Matteo
One hundred sixty-one Prototheca spp. strains isolated from composite milk and barn-surrounding
environmental samples (bedding, feces, drinking, or washing water, surface swabs) of 24 Italian dairy herds
were characterized by genotype-specific PCR analysis. Overall, 97.2% of strains isolated from composite milk
samples were characterized as Prototheca zopfii genotype 2, confirming its role as the main mastitis
pathogen, whereas Prototheca blaschkeae was only sporadically isolated (2.8%). Regarding environmental
sampling, 84.9% of isolates belonged to P. zopfii genotype 2, 13.2% to P. blaschkeae, and 1.9% to P. zopfii
genotype 1. The data herein contradict previous hypotheses about the supposed exclusive role of P. zopfii
genotype 2 as the causative agent of protothecal mastitis and, on the contrary, confirm the hypothesis that
such pathology could be caused by P. blaschkeae in a few instances.
ID nr. 4546
Maioli° G, Bonilauri° P, Spaggiari B, De_Filippo° F, Luppi° A, Calzolari° M, Dottori° M
Ticks infesting wild animals in Northern Italy
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 94
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
First author Maioli, Giulia
Pag. 262 di 300 31/08/2011 Background: The aim of this study was to provide more data on the Ixodid fauna of wildlife collected in Emilia
Romagna and Lombardia in order to better understand their ecology. Methods: Ixodid ticks were collected
from roe deer (Capreolus capreolus), wild boar (Sus scrofa), red deer (Cervus elaphus), fallow deer (Dama
dama), red fox (Vulpes vulpes), wolf (Canis lupus), hedgehog (Erinaceus europaeus), shrew (genus Sorex),
badger (Meles meles) and european brown hare (Lepus europaeus). Ticks were removed and identified
following taxonomic standard keys. Prevalence differences among host species, tick species and collection
period were tested by 2 test. Results: Ticks were collected from August 2008 until December 2009. A total of
3,225 ticks removed from 491 animals were identified. Ticks belonged to nine species: Ixodes ricinus
(n=2,222; 68.9%), Rhipicephalus sanguineus (n=647; 20%), Dermacentor marginatus (n=172; 5.3%), Ixodes
canisuga (n=119; 3.7%), I. hexagonus (n=53; 1.64%), Hyalomma marginatum (n=6; 0.18%), Haemaphisalys
punctata (n=3), I. acuminatus (n=2), Hae. concinna (n=1). I. ricinus tick represents the most frequently
detected species in our habitats. Also, we found that D. marginatus is often found on wild boar (34/44); I.
canisuga parasites fox only and, interestingly, we found some specimens of the brown dog tick (Rh.
sanguineus) on foxes, roe deer, wild boars and hares. Significant difference (p<0.01) was found in seasonality
for I. ricinus, Rh. sanguineus and D. marginatus, which were the more frequently sampled species .
Conclusions: Our data confirm that I. ricinus is the dominant species in roe deer, red deer and hares in Emilia
Romagna and Lombardia regions, while D. marginatus is strongly associated with wild boar. Passive
surveillance on hunted wild fauna could provide a useful and economic tool to collect data on ticks and to
achieve a better understanding of tick host preference for wild vertebrate species.
ID nr. 4547
Chiari° M, Cavadini° P, Barbieri° I, Grilli G, Ferrazzi V, Zanoni V, Gioia E, Capucci° L,
Lavazza° A
Data supporting the active role of cottontail (Sylvilagus floridanus) in the epidemiology of EBHS7
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 81
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
First author Chiari, Mario
Background: The eastern cottontail (Sylvilagus floridanus), an American lagomorph, was illegally introduced in
Piemonte on 1966 and is currently widespread in several areas of North-Central Italy. A seroepidemiological
survey was conducted in Alessandria province on 2000 to determine the role of cottontail as host or reservoir
of hares’ pathogens. As main outcome it was found that cottontails could have been naturally infected with
EBHSV, developing a specific immunity. Thereafter, the infection of seronegative cottontails proved their
susceptibility to EBHSV in experimental conditions. Aim of this work is an to report the results of serological
surveys for confirming the previous data in a more wide territory; b) to report the first natural EBHS outbreak
in a mixed population of hares and cottontail and c) the molecular characterization of the virus identified in
dead animals. Methods: From 2003 to 2009, 148 serum samples and 37 organs of cottontails, captured or
found dead in North- Central Italy were analyzed. Serological (cELISA) and virological (sandwich ELISA,
western blot and PCR) tests were performed using methods developed at the OIE Reference Laboratory. On
late 2009 an EBHS outbreak
occurred in a fenced area near Milano where a high density of both hares and cottontails was present.
Animals found dead were examined and the EBHSV identified strains from both species were amplified,
sequenced and compared. Results: Serological investigations confirmed the presence of positive anti-EBHS
titres in naturally infected cottontails. Moreover, during the EBHS outbreak, at least one cottontail found dead
show typical gross lesions and tested virologically positive for EBHS (liver and spleen). The viral strains from
hares and cottontail were amplified by PCR and VP60 products were sequenced, showing 100% identity.
Conclusions: It is even more evident that cottontail could be a natural host of EBHSV and may transmit it to
hares.
ID nr. 4548
Gelmini° L, Bassi° S, Carra° E, D’Incau° M, Fiocchi A, Sala L, Magistrali° L, Ferri M,
Pag. 263 di 300 31/08/2011 Zicavo° A, Pongolini° S
Salmonella Hessarek in starling (Sturnus vulgaris): diagnosis and molecular study of outbreak and
collection isolates
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 77
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
First author Gelmini, Luca
Background: Salmonella enterica subsp. enterica serovar Hessarek (S. Hessarek) belongs to serogroup B
and is considered a rare serovar. Firstly identified as a new serovar following isolation from a dead crow
(Corvus corax) in Iran, it has been mostly isolated from starling (Sturnus vulgaris) since, in this species it
exhibits high virulence giving rise to outbreaks sometimes characterized by significant mortality. Therefore, It
is considered a serovar with high host specificity. In this report we describe an outbreak of S. Hessarek
infection in starlings occurred in October 2009 in the city of Modena, Emilia-Romagna Region (Italy). Methods:
Thirty-two dead starlings were subjected to a diagnostic protocol including necropsy, bacteriological,
virological and parassitological examination. S. Hessarek was isolated from thirty-one birds and ten isolates
were genotyped by PFGE with XbaI and AvrII endonucleases. Further fourteen collection-isolates originating
from diverse places and time-periods were genotyped with the same method to assess the genetic variability
of this serovar and to elucidate possible epidemiological relationships among isolates. Results: Hepatomegaly
with small foci of necrosis in the liver, splenomegaly, focal haemorrhages of the pericardium and lungs and
intestinal congestion were observed at necropsy. S. Hessarek was isolated in pure culture from all tested
organs. Virological tests for Newcastle Disease, West-Nile Disease Flavivirus and type A avian influenza were
negative. Parassitological tests were negative for all animals but one. All ten outbreak isolates showed the
same XbaI and AvrII PFGE profiles. The fourteen collection strains were grouped into three different profiles
with both enzymes, among them six belonged to the same genotype as the outbreak isolates. Conclusions:
Our report confirms that S. Hessarek is a pathogen capable of causing an acute and deadly disease in
starling. Considerations regarding the genetic variability of tested isolates are presented in the poster.
ID nr. 4549
Spaggiari B, Marzi D, Merialdi° G, Cuccurese A, Massirio I, Anniballi F, Bano L, Defilippo°
F, Dottori° M
Clostridium botulinum type C outbreak in wild mammals in Italy
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 72
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
During the late summer of 2008 an uncommon increase of the mortality rate of different avian species was
observed along Crostolo and Rodano rivers in Emilia Romagna region, Italy (lat. 44°67’64’’N, long.
10°62’56’’E). The largest part of the affected birds was represented by mallards (Anas platyrhynchos). Other
species involved were little egret (Egretta garzetta), common kingfisher (Alcedo atthis), great cormorant
(Phalacrocorax carbo), common moorhen (Gallinula chloropus), yellow wagtail (Motacilla flava), rock pigeon
(Columba livia), European magpie (Pica pica), hooded crow (Corvus corone cornix) and common pheasant
(Phasianus colchicus). In addition to avian species, 21 dead coypus (Myocastor coypus) and 4 Norway rats
(Rattus norvegicus) were retrieved in the areas of the epizootic. Overall 26 birds, 4 coypus and 1 rat were
submitted for necropsy, bacteriological, toxicological and virological examinations. Sera collected from 3
moribund mallards and sera obtained from heart clots of dead mammals were filtered through a 0.22 µm filter
and tested for Clostridium botulinum neurotoxins by mouse test. Intestinal and liver samples collected from
birds and mammals carcasses were tested for the presence of C. botulinum by bacteriological procedures and
PCR for type A, B, C, D, E, F. All tested sera resulted positive for C. botulinum type C neurotoxin and C.
botulinum type C was isolated and detected by PCR. The remaining investigations resulted negative. C.
botulinum type C has been occasionally observed in mammals such as cattle, cats, dogs and horses but, to
the authors’ knowledge, this is the first report of natural acquired botulism in coypus and rats connected with
Pag. 264 di 300 31/08/2011 an avian botulism outbreak. Water and plant contamination from infected carcasses or maggots could have
lead to mammal species intoxication. This is especially true for coypus that are strictly vegetarian, while rats
intoxication could also have derived from the ingestion of maggots or from scavenging toxin-laden carcasses.
ID nr. 4550
Maioli° G, Fontana° MC, Luppi° A, Zanin D, Rugna° G, Renzi° M, Merialdi° G
Cluster of cysticercosis (Taenia pisiformis) in European brown hares in Northern Italy
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 61
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
Background: The cestode Taenia pisiformis (Bloch, 1780) occurs in the small intestine of domestic and wild
carnivores (dog, fox and rarely in the cat). The intermediate hosts are lagomorphs, primarily rabbits and hares
but also rodents. In this work the Authors report a cluster of cysticercosis due to Cysticercus pisiformis in
hares from an area where this pathology has been previously found sporadically. Methods: Between October
2008 and February 2010 47 brown hares, hunted or found dead in north area of Bologna province, were
collected and submitted to necropsy at the laboratories of IZSLER. Furthermore, 8 foxes from the same
location were necropsied and checked for the presence of intestinal helmints. Cysticerci and adult taenids
were morphologically identified following standard taxonomic keys. Results: At necropsy, 20 (42.5%) hares
from eight municipalities were found infested by C. pisiformis. These animals showed considerable weight
loss and bacteriological and virological investigations showed negative results. Cysticerci were found in liver,
abdominal cavity and mesentery of the lower digestive tract. All the animals were infested by more than 20
cysticerci. Most of the cases (10/20) were located in two neighbouring municipalities. Only 1 fox out of 8
examined carried two adults of T. pisiformis in the small intestine. The other 7 foxes were infested by
Mesocestoides lineatum and Toxocara canis. Conclusions: The increased prevalence of C. pisiformis in the
study area may have different causes such as: level of environment contamination, dispersion of eggs, egg
survival, age and immune response of the host, as well as densities of both definitive and intermediate hosts.
In the study area, the hare population decreased of 39,4% in 2008-2009, whilst the fox population increased.
Furthermore the practice allowing dog to eat the viscera of game animals may affect the prevalence of
infection in the hare population.
ID nr. 4551
Chiari° M, Zanoni° M, Salogni° C, Giovannini° S, Alborali° L, Lavazza° A
Presence of Trichinella britovi in a red fox (Vulpes vulpes) in Brescia Province (Italy)
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 66
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
First author Chiari, Mario
Background: In Italy foxes (Vulpes vulpes) are present and abundant everywhere and hold a high position in
the animal food chain. Since they represent the main indicator species of the local presence of Trichinella
spp., especially T. britovi, dead or hunted foxes are often used in monitoring programmes. Methods: In
Brescia province, the agreement among official veterinary service, public administrators and hunter
associations, made possible sampling the carcases of 228 foxes found death or hunted, between June 2009May 2010. Around 50% were coming from areas of > 400 mtr altitude. The presence of rabies was firstly
excluded; then, a pool of 10 gr of muscles (diaphragm, masseter, lower hind limb) was analysed for the
presence of Trichinella spp larvae. According to the EC 2075/2005 directives, Trichineasy (Syntec
International) method was used. Such machine grinds, digests and filters the samples and deposits the
material on a membrane filter, which is stained with a fluorescent reagent to detect trichina larvae. The
Pag. 265 di 300 31/08/2011 species identification was performed by the National Reference Laboratory for Trichinella using a specific
multiplex-PCR. Results: The prevalence was very low: larvae of Trichinella spp. were found in just one animal.
This was a young male, with characteristic lesions of sarcoptic mange, found dead in Sellero, a town in
Vallecamonica at 600 meters on sea level. The load was 45 larvae/gr of muscle analyzed and they were
further characterized as T. britovi. Conclusions: Whereas foxes have a low importance for meat consumption,
the absence of T. spiralis in wild carnivores is an essential step in the monitoring programs of pigs for
acquiring the status of free area at regional level. The presence of T. britovi is indicative of the circulation of
this zoonotic parasite in the wild circle even if both the prevalence (0.5%) and the age of infected animals
(young) were different than expected.
ID nr. 4552
Chiari° M, Zanoni° M, D'Incau° M, Salogni° C, Giovannini° S, Alborali° L, Lavazza° A
Isolation of Salmonella spp. from Red Fox (Vulpes vulpes) and Badger (Meles meles) in Lombardy
(North Italy)
9th conference of the European Wildlife Disease Association (EWDA) " Healthy wildlife, healthy people" :
Vlieland, The Netherlands, 13 to 16 September 2010 / [s.l. : s.n., 2010]. - p 49
Conference of the European Wildlife Disease Association (EWDA) (9th : Vlieland, The Netherlands : 13 to
16 September 2010)
First author Chiari, Mario
Background: Salmonella has been isolated from a wide variety of wild animals, both mammals and birds. It
may cause death in some of them, mainly small passerines, whereas some wild carnivores as red fox (Vulpes
vulpes) and badger (Meles meles) may act as indicator species to determine the presence of Salmonella in
the local environment. Methods: In Lombardy the agreement among official veterinary service, public
administrators and hunter associations, made possible sampling the carcases of foxes (Vulpes vulpes) and
badgers (Meles meles) found death or hunted, between June 2009-May 2010. The presence of rabies was
firstly excluded on all samples (511 red foxes and 18 badgers) and then further analysis were done.
Salmonella was isolated from faecal samples using both the mandatory methods for Salmonella monitoring
and control plan for primary productions (AnnexD ISO 6579:2002) and the in-house isolation procedure based
on the enrichment with Rappaport-Vassiliadis Broth and plating on Hecktoen enteric agar. Salmonella
identification was performed with biochemical tests (growing on TSI, ONPG test, identification by multitest kit)
and serotyping. Results: Salmonella was isolated from 29 foxes (6%) and 2 badgers. Sixteen different
serotypes were identified: 12/31 (39%) isolates belonged to serotypes (Typhimurium, Enteritidis,
Infantis)commonly found in men. Others serotypes could be either sporadically find in man (Derby, Muenchen,
Napoli, Livingston) or often find in water or in wild animals, in particular reptiles (Houtenae, Diarizonae,
Anatum, Veneziana). Conclusions: The behaviour and feeding habits of animals influence the likelihood of
being infected with Salmonella Foxes and badgers, that are at the top of the food chain in our region, could be
infected by eating carcasses contaminated by Salmonella or by different anthropogenic environmental
contamination, as foodstuff residues. Therefore, wild carnivores are an important reservoir of pathogenic
serotypes of Salmonella, and may be a risk for human and livestock.
ID nr. 4553
Calzolari° M, Bonilauri° P, Bellini R, Albieri A, Defilippo° F, Maioli° G, Galletti° G, Gelati A,
Barbieri° I, Tamba° M, Lelli° D, Carra° E, Cordioli° P, Angelini° P, Dottori° M
Evidence of simultaneous circulation of West Nile and Usutu Viruses in mosquitoes sampled in
Emilia-Romagna Region (Italy) in 2009
PLoS One. - Vol. 5 no 12 ( 2010). - p e14324. - 52 bib ref
Impact factor: 4,351
Project PRF2007303 STRAT P3 - Zoonosis and exotic viral diseases : one medicine approach ( human and
vetrinary ) P3 Chikungunya virus infection: epidemiological and clinical features - Caprioli dr. Alfredo
- UO - MINSAN - 2007 - 2500000,00 EUR - 01/07/2009 0.00.00 - 30/06/2011 0.00.00 - 1 - N8 -
Pag. 266 di 300 31/08/2011 Sezione diagnostica provinciale di Reggio Emilia - Dottori Dr. Michele - I
First author Calzolari, Mattia
In recent years human diseases due to mosquito-borne viruses were increasingly reported in Emilia-Romagna
region (Italy), from the chikungunya virus in 2007 to the West Nile virus (WNV) in 2008. An extensive
entomological survey was performed in 2009 to establish the presence and distribution of mosquito
arboviruses in this region, with particular reference to flaviviruses. Methodology/Principal Findings From May
6 to October 31, a total of 190,516 mosquitoes were sampled in georeferenced stations, grouped in 1,789
pools according date of collection, location, and species, and analyzed by reverse transcription polymerase
chain reaction (RT-PCR) to detect the presence of RNA belong to Flavivirus genus. WNV was detected in 27
mosquito pools, producing sequences similar to those of birds and human strains obtained in 2008 outbreak,
pointed out the probable virus overwintering. Isolation of WNV was achieved from one of these pools.
Moreover 56 pools of mosquitoes tested positive for Usutu virus (USUV). Most PCR positive pools consisted
of Culex pipiens, which also was the most analyzed mosquito species (81.4% of specimens); interestingly,
USUV RNA was also found in two Aedes albopictus mosquito pools. Simultaneous circulation of WNV and
USUV in the survey area was highlighted by occurrence of 8 mosquito WNV- and USUV-positive pools and by
the overlaying of the viruses “hot spots”, obtained by kernel density estimation (KDE) analysis. Land use of
sampled stations pointed out a higher proportion of WNV-positive Cx. pipiens pool in rural environments
respect the provenience of total sampled pool, while the USUV-positive pools were uniformly captured in the
different environments. Conclusions/Significance Obtained data highlighting the possible role of Cx. pipiens
mosquito as the main vector for WNV and USUV in Northern Italy, and the possible involvement of Ae.
albopictus mosquito in USUV cycle. The described mosquito-based surveillance could constitute the
foundation for a public health alert system targeting mosquito borne arboviruses.
ID nr. 4554
Ciminiello P, Dell'Aversano C, Fattorusso E, Forino M, Tartaglione L, Boschetti L, Rubini°
S, Cangini M, Pigozzi S, Poletti R
Complex toxin profile of Mytilus galloprovincialis from the Adriatic sea revealed by LC–MS
Toxicon. - Vol. 55 ( 2010). - p 280-288. - 36 bib ref
Impact factor: 2,128
This paper reports on the determination of toxin profile of mussels (Mytilus galloprovincialis) collected in
November–December 2003 along the Emilia Romagna coasts (Italy) when a high concentration of
Alexandrium ostenfeldii cells was detected in seawater. Detailed liquid chromatography–mass spectrometry
(LC–MS) analyses were performed on the crude extracts in both selected ion monitoring (SIM) and multiple
reaction monitoring (MRM) modes. They revealed that M. galloprovincialis had accumulated the three major
spirolides produced by the alga, namely 13-desMethyl spirolide C, 13,19-didesMethyl spirolide C and 27hydroxy-13,19-didesMethyl spirolide C, which fully accounted for toxicity of lipophilic extracts shown in mouse
bioassay. Interestingly, yessotoxin (YTX) and its analogues were still present in mussel polar extracts but YTX
itself was not the major toxin contained in mussels. The presence of pectenotoxin-2 seco acid (PTX-2sa) and
its putative epimer was also assessed. The presence of azaspiracids, never reported from the Adriatic sea, as
well as of diarrhetic shellfish poisoning toxins (okadaic acid, dinophysistoxins and OA esters) and domoic
acid, long known as contaminants of Adriatic mussels, was also investigated.
ID nr. 4558
Calistri P, Giovannini A, Savini G, Bonfanti l, Cordioli° P, Lelli R, Caporale V
Antibody response in cattle vaccinated against bluetongue serotype 8 in Italy
Transboundary Emerg Dis. - Vol. 57 no 3 ( 2010). - p 180-184. - 28 bib ref
Impact factor: 1,854
To assess the immunogenicity of Zulvac® 8 Bovis (a commercial inactivated vaccine against bluetongue virus
Pag. 267 di 300 31/08/2011 serotype 8 – BTV8) under field conditions, 71 cattle vaccinated according to manufacturer schedule in Verona
province (Italy) were tested for the presence of BTV8 neutralizing antibodies at 21, 29, 36, 43, 49, 102 and
201 days post-vaccination (dpv). Another group of 528 BTV8 vaccinated cattle in Mantova province (Italy) was
also tested once between 113 and 174 dpv. The vaccine was able to elicit an immune response in 69 (97.2%)
and 346 (65.5%) animals of the Verona and Mantova groups, respectively
.
ID nr. 4559
Calistri P, Giovannini A, Savini G, Monaco F, Bonfanti L, Ceolin C, Terregino C, Tamba°
M, Cordioli° P, Lelli R
West Nile Virus transmission in 2008 in North-Eastern Italy
Zoonoses Public Health. - Vol. 57 no 3 ( 2010). - p 211-219. - 33 bib ref
Impact factor: 1,912
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
After 10 years, West Nile virus (WNV) re-emerged in Italy in August 2008. As on 31 December 2008, the
infection affected eight Provinces in three Regions (Emilia Romagna, Veneto, Lombardy), where a total of 794
cases of WNV infection in 251 equine stables were detected on the basis of the clinical signs and as a result
of a serological screening in horses living in the area. Only 4.0% (32/794) of the serologically positive animals
showed clinical signs, and the 32 clinical cases were reported in 18 different farms. The observed case-fatality
rate was 15.6% (5/32). The confirmed clinical cases were detected from end August to mid October.
Significant levels of positivity by RT-PCR were also observed in magpies (Pica pica) (9.1%, 95% confidence
levels: 6.1–13.4%), carrion crows (Corvus corone) (7.4%, 95% confidence levels: 3.6–14.4%) and rock
pigeons (Columba livia) (12.9%, 95% confidence levels: 7.6–21.2%).
ID nr. 4560
Piccirillo A, Pasotto D, Martin° AM, Cordioli° P
Serological survey for influenza type A viruses in domestic dogs (Canis lupus familiaris) and cats
(Felis catus) in North-Eastern Italy
Zoonoses Public Health. - Vol. 57 no 4 ( 2010). - p 239-243. - 33 bib ref
Impact factor: 1,912
To ascertain the potential transmission of influenza A viruses to dogs and cats, a serological survey was
carried out in North-eastern Italy. In a 4-year period, 637 serum samples were screened using a Mab-based
competitive ELISA for anti-nucleoprotein A (NPA) antibody detection of influenza viruses. No evidence of antiNPA antibodies was observed.
ID nr. 4561
Elia G, Decaro N, Martella V, Lorusso E, Mari V, Lucente SM, Cordioli° P, Buonavoglia C
An ELISA based on recombinant spike protein S for the detection of antibodies to transmissible
gastroenteritis virus of swine-like canine coronaviruses
Pag. 268 di 300 31/08/2011 J Virol Methods. - Vol. 163 ( 2010). - p 309-312. - 19 bib ref
Impact factor: 2,133
Project PRF2007202 - Mammal coronaviruses: molecular epidemiology, vaccine development And
implications for animal and human health - Cordioli Dr. Paolo - Capofila - MINSAN - 2007 300000,00 EUR - 01/09/2009 0.00.00 - 31/08/2011 0.00.00 - 1 - U1 - REPARTO VIROLOGIA Cordioli Dr. Paolo - I
2 - Università di Bari - Dipartimento di Sanità Pubblica e Zootecnica - Decaro Prof. Nicola - E
3 - IstitutoZooprofilattico della Sardegna - Oggiano Dr.ssa Annalisa - E
4 - Istituto Zooprofilattico della Sicilia - Guercio Dr.ssa Annalisa - E
5 - Istituo Zooprofilattico delle Venezie - Nardelli Dr. Stefano - E
Recombinant canine coronaviruses, resembling the transmissible gastroenteritis virus of swine (TGEV) in a 5'
fragment of the S glycoprotein, have been detected recently and showed to be present in canine populations.
The 5' fragment of the S protein (S') of a TGEV-like canine coronavirus (CCoV), strain 174/06, was expressed
in an Escherichia coli cell-free system. The purified recombinant polypeptide was employed to develop an
ELISA test for the detection of TGEV-like CCoV-specific antibodies in dog sera. Four canine sera positive for
TGEV-like CCoV, six sera positive to classical CCoV-II strains and 10 negative control sera were examined.
The recombinant S' was not recognized by antibodies to classical CCoV-II, as only sera from dogs infected
experimentally with TGEV-like CCoV reacted strongly with the recombinant S' polypeptide whereas dog sera
with antibodies to classical CCoV-II did not react. As classical CCoV-II and TEGV-like CCoVs are related
antigenically, the recombinant S' ELISA is a useful method to investigate serologically the prevalence of
TGEV-like CCoVs in dogs.
ID nr. 4566
Moreno° AM, Barbieri° I, Chiapponi° C, Sozzi° E, Foni° E, Zanoni° MG, Lelli° D, Cordioli°
P
Genetic evolution of italian H1N2 swine influenza virus during 1998-2009: presence of new
reassortant strains
4th annual meeting Epizone : "Bridges to the future" : 7-10 June, 2010 Saint-Malo, France : abstracts oral
and poster presentations / [s.l. : s. n., c2010]. - p 74. - 4 bib ref
Annual meeting Epizone (4th : Saint-Malo, France : 7-10 June, 2010)
First author Moreno_Martin, Ana
ID nr. 4567
Razzuoli E, Villa R, Sozzi E, Amadori° M
RT-Real Time PCR for detection of porcine alpha and beta interferon genes
5th Epizone theme 5 meeting : November 3-5, 2010 Copenhagen / [s.l. : s. n., c2010]. - p 15
Epizone theme 5 meeting (5th : Copenhagen : 3-5 November, 2010)
Project EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 14000000,00 EUR - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 17 - U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia I
First author Amadori, Massimo
A few studies provided convincing evidence of constitutive expression of type I interferons (IFNs) in humans
and mice, and of the steady-state role of these cytokines under health conditions. These results were later
confirmed in pigs, too. In line with this tenet, low levels of IFN aiphalbeta can be detected in swine tissues in
the absence of any specific inducer. These studies are compounded by the utmost complexity of type 1 IFN
system (including among others 17 IFN-alpha genes in pigs), which demands proper research tools. This
prompted us to anaiyze the availabie protocols and to develop a relevant, robust reverse transcription (RT),
Real Time PCR detection system for amplification of ali porcine IFN aiphalbeta genes. The adopted test
procedure is user-friendly and provides the panel of gene expression of one subject in a microtitre piate. Also,
Pag. 269 di 300 31/08/2011 a proper use of PCR fluorochromes (SYBR vs. EVA Green supermix) enables users to shift from qualitative to
quantitative test protocols, in case of low-expression IFN genes like IFN A3. This is accounted for by the much
higher sensitivity of the test protocol with EVA Green supermix. Interestingly, IFN beta showed the highest
frequency of expression in line with its definition of "immediate early" gene in humans and mice. Results
indicate that the outiined procedure can detect both constitutively expressed and virus-induced IFN aiphalbeta
genes, as well as the impact of environmental, non-infectious stressors on the previous profile of constitutive,
Type I IFN expression..
ID nr. 4568
Gamba° V, Benetti C, Buratti R, Curatolo° M, Galarini R
Development and validation of a screening ELISA test for the determination of ten quinolones in
muscle, egg and milk
6th Intenational Symposium on Hormone and Veterinary Drug Residue Analysis : Ghent, Belgium, June 1-4,
2010 : abstract book / [s.l. : s.n., 2010]. - p 97
Intenational Symposium on Hormone and Veterinary Drug Residue Analysis (6th. : Ghent, Belgium : June
1-4, 2010)
First author Gamba, Daniela
Quinolones are a group of synthetic broad-spectrum antibiotics, widely used for the prevention and the
treatment of various diseases in husbandry animals and acquaculture. In the EU legislation the use of
quinolones is allowed. Their maximum residue limits (MRL) have been set at different values in various
matrices, but no limits have been set for eggs. In recent years several works on LC-FLD and LC-MS/MS
analysis of quinolones bave been published. However, in laboratories with high throughput, the availability of
simple, quick and sensitive screening tests is necessary. Immunoassay techniques meet these requirements
and, at the same time, are cheap. The aim of this work is the development of a simple qualitative screening
method for quinolones in various matrices of animal origin (i.e. muscle, milk and egg), using a commercial
ELISA test (r-Biopharm), and the evaluation of its analytical performances according to the European Decision
2002/657/EC criteria. The cross-reactivity of all analytes was verified at 25 µg/kg for muscle and egg and at
15 µg/kg for milk samples. Sarafloxacin has then been selected as reference compound for the validation
study. The method performances are studied by three laboratories involved in official residues control
programs. Difficulties encountered during validation, results and data analysis are discussed.
ID nr. 4569
Foni° E, Bublot M, Chiapponi° C, Herin JB, Joisel F, Perreul G, Zanni° I, Vila T
Seroprevalence of H1N1, H3N2 and H1N2 influenza virus in 29 pig farms in France in 2009
21st International Pig Veterinary Society Congress (IPVS) : July 18 – 21, 2010 Vancouver, Canada :
proceedings / [s.l : s.n, c2010]. - cdrom p 565. - 4 bib ref
International Pig Veterinary Society Congress (IPVS) (21st : Vancouver, Canada : July 18 – 21, 2010)
First author Foni, Emanuela
ID nr. 4571
Brookes S, Núñez A, Choudhury B, Matrosovich M, Essen SC, Clifford D, Slomka MJ,
Kuntz-Simon G, Garcon F, Nash B, Hanna A, Heegaard PMH, Quéguiner S, Chiapponi°
C, Bublot M, Garcia JM, Gardner R, Foni° E, Loeffen W, Larsen L, Van_Reeth K, Banks J,
Irvine RM, Brown IH
Replication, pathogenesis and transmission of pandemic (H1N1) 2009 virus in non-immune pigs
PLoS One. - Vol. 5 no 2 ( 2010). - p e9068. - 35 bib ref
Impact factor: 4,351
Pag. 270 di 300 31/08/2011 The declaration of the human influenza A pandemic (H1N1) 2009 (H1N1/09) raised important questions,
including origin and host range [1,2]. Two of the three pandemics in the last century resulted in the spread of
virus to pigs (H1N1, 1918; H3N2, 1968) with subsequent independent establishment and evolution within
swine worldwide [3]. A key public and veterinary health consideration in the context of the evolving pandemic
is whether the H1N1/09 virus could become established in pig populations [4]. We performed an infection and
transmission study in pigs with A/California/07/09. In combination, clinical, pathological, modified influenza A
matrix gene real time RT-PCR and viral genomic analyses have shown that infection results in the induction of
clinical signs, viral pathogenesis restricted to the respiratory tract, infection dynamics consistent with endemic
strains of influenza A in pigs, virus transmissibility between pigs and virus-host adaptation events. Our results
demonstrate that extant H1N1/09 is fully capable of becoming established in global pig populations. We also
show the roles of viral receptor specificity in both transmission and tissue tropism. Remarkably, following
direct inoculation of pigs with virus quasispecies differing by amino acid substitutions in the haemagglutinin
receptor-binding site, only virus with aspartic acid at position 225 (225D) was detected in nasal secretions of
contact infected pigs. In contrast, in lower respiratory tract samples from directly inoculated pigs, with clearly
demonstrable pulmonary pathology, there was apparent selection of a virus variant with glycine (225G).
These findings provide potential clues to the existence and biological significance of viral receptor-binding
variants with 225D and 225G during the 1918 pandemic.
ID nr. 4572
Bellini° S, Alborali° L, Zanardi° G, Bonazza° V, Brocchi° E
Swine vesicular disease in northern Italy: diffusion through densely populated pig areas
Rev Sci Tech OIE. - Vol. 29 no 3 ( 2010). - p 639-648. - 13 bib ref
Impact factor: 1,238
First author Bellini, Silvia
At the end of 2006, a recrudescence of swine vesicular disease (SVD) was recorded in Italy and the disease
spread widely throughout the northern regions. Lombardy, a densely populated pig area, was most affected
and the presence of the disease caused heavy economic losses to the entire pig industry. Although SVD is
considered only moderately contagious, the epidemic in the north was characterised by a rapid spread of the
condition. Numerous difficulties were encountered in eradicating it. Over the past decade, there has been a
significant increase in the population of pigs in Lombardy, concentrated mainly in a few areas which were the
most severely affected during the 2006 to 2007 SVD epidemic. Increases in both the pig population and
animal movements, combined with weak biosecurity measures, increased the spread rate of the disease and
hampered eradication activities.
ID nr. 4573
Fiocchi A, Gustinelli A, Trentini M, Bassi° S, Gelmini° L, Fioravanti° ML
Gastrointestinal helminths in bird of prey from Italy
Parassitologia. - Vol. 52 no 1-2 ( 2010). - p 271
Congresso Nazionale della Societa' Italiana di Parassitologia (26. : Perugia : 22-25 giugno 2010)
First author Fiocchi, A
ID nr. 4574
Carra° E, Pongolini° S, Carpana° E, Paganelli° GL, Rugna° G, Bassi° S
Real-time PCR method for the quantification of Paenibacillus larvae spores in debris: comparison to
microbiology
4th European Conference of Apidology EurBee : 7-9 September 2010 Metu-Ankara, Turkey : proceedings /
[s.l. : s.n., 2010]. - p 118
European Conference of Apidology EurBee (4th : Metu-Ankara, Turkey : 7-9 September 2010)
Pag. 271 di 300 31/08/2011 Project APENET - Evaluation of the synergism among diverse factors on bee health - Bortolotti Dr.ssa
Laura - UO - ALTRI - 2009 - 478400,00 EUR - 01/12/2009 0.00.00 - 31/03/2011 0.00.00 - 1 N4 - Sezione diagnostica provinciale di Modena - Bassi Dr. Stefano - I
First author Carra, Elena
American foulbrood is the most diffused and severe bacterial disease of the honeybee brood. It is caused by
the spore-forming bacterium Paenibacillus larvae (P. larvae). The control of the disease relies essentially on
the identification and elimination of diseased colonies. Disclosure of latent infections in colonies without
symptoms, by detection of P. larvae spores, is an important component of American foulbrood prevention.
Honey, adult bees and winter debris are the most useful targets to assess the level of contamination of
colonies. The aim of our work was the development of a real-time PCR method for the quantitative
assessment of the spore content in winter debris and the comparison of this molecular method to the
microbiological enumeration. A new TagMan® real-time PCR was designed targeting the 16S-ribosomal RNA
gene of P. larvae, taking particular care of reaction specificity. For this purpose both in-silico and in-vitro
specificity were tested with respect to several potential bacterial contaminants of the hive, belonging to
Paenibacillus, Bacillus and Enterococcus genera. The same real-time PCR was used to screen for positive
samples of debris and to quantify the spores in samples resulted positive on screening. Microbiological spore
counting was performed by plating onto MYPGP-agar supplemented with nalidixic and pipemidic acid, after
distilled-water extraction of samples. Winter debris from approximately 150 colonies belonging to 15 apiaries
of three provinces in Northern Italy were analysed. Selected apiaries had shown different disease levels in the
year preceding the study. The concordance level of the tested methods is discussed.
ID nr. 4575
Dall'Ara A, Golfari G, Massi° P, Poglayen G
Manure hygienisation developement and application (MIDA) as biological technology for treatment of
laying ben manure
International Poultry Scientific Forum : January 25-26 2010 : abstracts / [s.l. : s.n., 2010]. - p 54
International Poultry Scientific Forum : January 25-26 2010)
Overall, the goal is to overcome the management of manure as waste and the agricultural lands need for its
spreading through the direct production of quality hygienic fertilizer, that improves the soil structure, enhances
its fertility and rehabilitates for predesertification. In details, the aim of the project was to verify laying hen
manure (LHM) hygienisation by bio-treatment, according to Reg. (CE) 1774/2002 indications. The obtained
product, should be transformed not in a waste, but in an hygienic and marketable safe organic fertilizer. LHM
dried at three different level of humidity by MDS (Manure Drying System) was treated inside big-bags and pile,
by adding PAV (Vegetal Active Principles) a biocatalyst of plant origin, in a poultry farro in Italy. Manure
sampling and analysis, taken at days -3 (before MDS), 0, 38, 81, 123, were carried out following Reg. (CE)
1774/2002 and taking irato account also the chemical nutrients considered in D. Lgs. 217/06 Objectives for
thermal and chemical process are: - absence of Salmonella spp.in 25 grams; - concentration of Escherichia
coli in 1 gram lower than or equal to 1000 u.f.c.; - reduction S log 10 of Enterococcus faecalis in 1 gram; reduction 3 log 10 of Parvovirus. In this early stage, due to the high environmental resistence of dispersal
stages of parasites, we have added also the evaluation of PAV's activity on coccidia and ascarid eggs. This
justified the sampling at day -3 useful to assess the parasites burden in the fresh LHM. Also pathogen and
saprophytic fungi were searched with the same timing. Due to the absence of Parvovirus in poultry, to follow
the reg. (CE) 1774/2002 we have developed a "daughter" trial in controlled conditions adding Parvovirus of
swine origin to LHM. From a general point of view, the experimental activity of MIDA has shown good results
in reducing biologica] parameters after 123 days and appears to be encouraging for the authors to go on,
developing the project and searching for financial support.
ID nr. 4576
Salogni° C, Cervellione F, Zanoni° M, Giovannini° S, Alborali° GL
Yersina ruckeri in siberian sturgeon (Acipenser baeri). Discription of a outbreak of infection in a
Pag. 272 di 300 31/08/2011 Northern Italy fish farm
XVI Convegno Nazionale Società Italiana di Patologia Ittica (SIPI) : 27-29 Maggio 2010, Orvieto (TR) : atti /
[s.l. : s.n., 2010]. - p 30
Convegno Nazionale Società Italiana di Patologia Ittica (SIPI) (16. : Orvieto (TR) : 27-29 Maggio 2010)
First author Salogni, Cristian
This communication describes an outbreak of infection with Yersinia ruckeri in Siberian sturgeon (Acipenser
baeri) occurred in May of 2009,in a fish farm in northern Italy and characterized by a 1-3%, daily mortality
which lasted for about a fortnight to reach a cumulative value of 30%. The entire lot of fish (20,000 subjects),
the average weight of 40 g, previously had been imported from Eastern Europe. The symptomatology was
characterized by apathy, loss of appetite, moderate bilateral popeye, skin darkness, bleeding of the oral cavity
at the base of the fins and in the perineal region. The autopsy showed Gill pallor, coelomic serumhemorrhagic exudate, congestion and increase in volume of the spleen, distension of the bladder for storage
of gas and catarrhal-haemorrhagic enteritis. The cultural examination, performed on blood agar and TSA,
allowed the isolation of pure colonies from all organs in all subjects tested (septicemic form). Identification of
the germ isolated as Yersinia ruckeri was performed by biochemical characterization (Macro, miniaturized
system API20E), genomics and serological survey. This work represents the first report on the national
territory, and one of the few in the world, and infection of the pathogenic role of Yersinia ruckeri in the Siberian
sturgeon.
ID nr. 4577
Calzolari° M, Bonilauri° P, Albieri A, Bellini R, Carra° E, Defilippo° F, Maioli° G, Tamba° M,
Angelini P, Dottori° M
Evidence in West Nile virus and Usutu virus circulation in mosquitoes sampled in Emilia-Romagna
Region in 2009
4th annual meeting Epizone : "Bridges to the future" : 7-10 June, 2010 Saint-Malo, France : abstracts oral
and poster presentations / [s.l. : s. n., c2010]. - p 204. - 7 bib ref
Annual meeting Epizone (4th : Saint-Malo, France : 7-10 June, 2010)
Project PRC2008005 - Mapping Arthropod Vectors: on field evaluation of different monitoring methods Tamba Dr. Marco - Capofila - MINSAN - 2008 - 187000,00 EUR - 01/10/2009 0.00.00 30/09/2012 0.00.00 - 1 - N0 - CEREV - Tamba Dr. Marco - I
2 - N8 - Sezione diagnostica provinciale di Reggio Emilia - Luppi dr. Andrea - I
3 - M8 - Sezione diagnostica provinciale di Pavia - Fabbi Dr. Massimo - I
4 - G1 - Reparto di Virologia e Sierologia Specializzata - Lelli dr. Davide - I
5 - Centro Agricoltura Ambiente "G. NIcoli" - Bellini dr. Romeo - E
6 - Istituto Zoop. della Sicilia - Torina dr.ssa Alessandra - E
EPIZONE - "Network of excellence for epizootic disease diagnosis and control - "EPIZONE". - Van
Rijn dr. Piet - UO - CE - 2005 - 01/06/2006 0.00.00 - 31/05/2011 0.00.00 - 14000000,00 EUR 17
- U3 - REPARTO AGENTI AD ALTA DIFFUSIONE E BIOTECNOLOGIE - Bellini Dr.ssa Silvia - I
First author Calzolari, Mattia
ID nr. 4578
Gale P, Stephenson B, Younas A, Ulrich RG, Lindstrom A, Wahistrom H, Scremin M,
Martinez M, De_La_Torre A, Munoz MJ, Bosch J, Capelli G, Phipps P, Dottori° M,
Bonilauri° P, Wilson A, Yin H
Towards a qualitative spatial model for risk of incursion of Crimean-Congo haemmorrhagic fever in
livestok in Europe
4th annual meeting Epizone : "Bridges to the fu