Fatty acid methyl esters, C18
Analysis of trans fatty acids in refined
vegetable oil
Application Note
Food Testing & Agriculture
Authors
Introduction
Agilent Technologies, Inc.
Gas chromatography with an Agilent CP-Wax 52 CB column identifies ten trans fatty
acids formed during the refining of lampante olive oil.
Conditions
Peak identification
Technique
: GC-capillary
Column
: Agilent CP-Sil 88 for Fames, 0.25 mm x 50 m fused
silica WCOT CP-Sil 88 (df = 0.2 μm)
(Part no. CP7488)
Temperature
: 70 °C (10 min) → 200 °C, 3 °C/min
Carrier Gas
: H2, 70 kPa (0 .7 bar, 10 psi)
Injector
: Splitter
T = 250 °C
Detector
: FlD
T = 250 °C
Courtesy
: C. Mariani et al,
Stazione sperimentale per le industrie degli di e dei
grassi, Milano, Italy
1. C15:1 trans-isomers
2. trans-trans
C18:2 3. cis-trans
C18:2 4. trans-cis
C18:2 5. cis-cis
C18:2 6. cis-trans
C18:2 7. cis-cis-trans
C18:3 8. cis-trans-cis
C18:3 9. trans-cis-cis
C18:3 10. cis-cis-cis C18:3 (linoleic acid)
(linoleic acid)
(linoleic acid)
(linoleic acid)
(linoleic acid)
(linolenic acid)
(linolenic acid)
(linolenic acid)
(linolenic acid)
www.agilent.com/chem
This information is subject to change without notice.
© Agilent Technologies, Inc. 2011
Printed in the USA
31 October, 2011
First published prior to 11 May, 2010
A00658