FTA® Bacterial
DNA Whatman
Raccolta, trasferimento,
stoccaggio e purificazione
di DNA batterico
La tecnologia FTA della Whatman offre un valido e semplice
metodo per la preparazione di campioni per analisi
genetiche da batteri. Occorre semplicemente applicare
campioni di colture o campioni clinici sulla matrice FTA il
DNA viene catturato e stabilizzato all’istante, consentendone
lo stoccaggio a temperatura ambiente, da analizzare al
momento più opportuno. Gli agenti patogeni vengono
inattivati all’istante. Trasporto sicuro e purificazione in 30
minuti rendono la tecnologia FTA un indispensabile
strumento di ricerca.
Caratteristiche Principali
• Indicato per un’ampia varietà di
batteri Il DNA genomico pronto
per la PCR può essere isolato
rapidamente da una varietà di
batteri gram-negativi e grampositivi, batteri spore-formanti e
acido-resistenti senza alcun o
minimo pretrattamento.
• Rapida purificazione Il DNA viene
purificato sulla FTA Card in tre
semplici steps, in un singolo tubo, a
temperatura ambiente. Il DNA
rimane immobilizzato sulla matrice
ed è subito pronto per la PCR. Se è
necessario avere DNA in soluzione si
può usare Whatman GenSpin.
• Raccolta semplice Depositare
colture o campioni clinici
direttamente sulla matrice FTA (FTA
Card Indicatrice).
• Manipolazione e trasferimento
sicuri FTA inattiva gli agenti
patogeni potenzialmente nocivi.
• Perfetto per il vostro metodo
FTA funziona, qualsiasi il metodo
scelto per l’identificazione di
batteri: campioni da colture pure di
batteri o campioni clinici.
• Temperatura ambiente Gli acidi
nucleici vengono automaticamente
stabilizzati senza la necessità di
refrigerazione.
• Automatizzazione L’utilizzo di
sistemi automatici accelera le fasi di
preparazione di diversi dischi
prelevati contemporaneamente,
ottimizza le risorse disponibili e
standardizza la purificazione di
DNA. I dischi prelevati dalla FTA
Card possono essere sottoposti alle
fasi di lavaggio e preparazione per
la PCR su una diversi tipi di
strumenti presenti in commercio
(liquid handling robot).
Applicazioni
• Applicazioni diagnostiche
• Monitoraggio acqua/aria
• Analisi biologiche
• Identificazioni molecolari
• Analisi di alimenti
• Applicazioni di ricerca
• Analisi ambientali
Tre semplici passaggi operativi
per ottenere un DNA puro
Dispensare il campione
Prelevare un
piccolo disco
(punch) dal
Campione
Purificare
PROTOCOLLO APPLICATIVO DEL FTA BACTERICAL DNA
Dispensare il campione
Dispensare il campione sulla
FTA Card. Lasciarlo
asciugare completamente.
Se si utilizza una Card
Indicatrice, l’area coperta
dal campione cambierà
colore da rosa a bianco
indicando la posizione del
campione stesso.
Lavaggio con soluzione
tampone TE-1
Effettuare due lavaggi con
la soluzione tampone TE-1
(10mM Tris, 0,1 mM EDTA,
pH 8,0). Eliminare la
soluzione usata dopo
ciascun lavaggio.
Essiccazione
Lasciar asciugare il disco nel
tubo PCR
Prelevare un
piccolo disco
Prelevare (mediante
perforazione) un piccolo
disco dal campione
depositato sulla FTA Card.
PCR
Aggiungere la master mix
per la PCR direttamente al
disco e iniziare la reazione
di amplificazione.
Purificazione con
Reagente di Purificazione
FTA (Purification Reagent
FTA)
Mettere il disco di campione
in un tubo per PCR ed
effettuare tre lavaggi con il
Reagente di Purificazione
FTA. Eliminare la soluzione
utilizzata dopo ciascun
lavaggio.
Qualità Whatman
Whatman, azienda leader nelle tecnologie per la separazione,
è nota nellà comunità scientifica per prodotti e soluzioni
innovative contraddistinte da un’elevata qualità. Il nostro
istinto per la semplificazione accelera la velocità di fare nuove
scoperte, riduce i costi e risparmia tempo. Inoltre, per
accentuare ulteriormente l’impegno nel soddisfare le
specifiche esigenze dei nostri clienti, Whatman è organizzata
in quattro aree distinte: LabScience, BioScience, MedTech
Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono
disponibili collegandovi al sito www.whatman.com.
FTA, GenSpin e Whatman sono marchi registrati della
Whatman Group.
INFORMAZIONI PER L’ORDINE DAL CATALOGO WHATMAN
No. Cat.
Descrizione
Quantità
per Confezione
WB120061
WB120204
WB120205
WB120206
WB120055
WB120056
WB120210
WB120211
WB100005
WB100006
WB100028
WB100010
WB100011
WB100025
WB100003
WB100016
WB100020
WB120005
FTA Starter Pack
FTA Reagente di Purificazione
FTA Classic Card (non-indicatrice)
FTA Classic Card (indicatrice)
FTA Mini Card (non-indicatrice)
FTA Mini Card (indicatrice)
FTA Micro Card (non-indicatrice)
FTA Micro Card (indicatrice)
Harris Micro Perforatore 1.2mm
Harris Micro Perforatore 1.2mm Tip
Harris Uni-Core 1.25mm Perforatore
Busta Multi-Barrier (grande)
Busta Multi-Barrier (piccola)
1.2mm Plunger di Ricambio
Essiccante (1g)
Busta Postale per FTA Card
Tagliere di Ricambio
GenSpin Kit di Purificazione DNA
1
500mL
100
100
100
100
100
100
1
1
4
500
500
1
1000
50
1
50 Purificazioni
Purificazione del DNA in meno di 25
minuti! Il GenSpin Kit di Purificazione
del DNA della Whatman rappresenta un
metodo estremamente semplice per
purificare il DNA a singolo filamento da
campioni di sangue e cellule in coltura. Il
campione è subito pronto per la PCR a
partire anche da piccoli volumi del
campione di partenza.
North America Whatman Inc
9 Bridewell Place,
Clifton, NJ 07014
Technical Support: 1-800-922-0361
Customer Service: 1-800-631-7290
Outside US: 973-773-5800
Fax: 973-773-0168
E-mail: [email protected]
Europe Whatman International Ltd
Springfield Mill
James Whatman Way
Maidstone
Kent ME14 2LE, UK
Tel: +44 (0)1622 676670
Fax: +44 (0)1622 691425
E-mail: [email protected]
Japan Whatman Japan KK
Daiwa Ueno Building 1F
6-10 Ueno 5-chome, Taito-ku
Tokyo 110-0005, Japan
Tel: +81 (0)3 3832 6707
Fax: +81 (0)3 3832 6457
E-mail: [email protected]
Asia Pacific Whatman Asia Pacific Pte Ltd
171 Chin Swee Road
#08-01 San Centre
Singapore 169877
Tel: +65 6534 0138
Fax: +65 6534 2166
E-mail: [email protected]
GenSpinTM Kit di Purificazione DNA Whatman
Leaders in Separations Technology
www.whatman.com
Cat No. S9036-782
FTA Cards and Indicating FTA Cards
WHATMAN CATALOGUE ORDERING INFORMATION
Catalogue
Number
Description
Sample
Cards/ Areas/
Pack
Card
Maximium
Volume/
Sample Area
(µL)
Maximium
Total
Volume/Card
(µL)
Whatman Quality
WB120205
FTA Classic Card
100
4
125
500
WB120206
Indicating FTA
Classic Card*
100
4
125
500
WB120055
FTA Mini Card
100
2
125
250
WB120056
Indicating FTA
Mini Card*
100
2
125
250
WB120210
FTA Micro Card
100
1
125
125
WB120211
Indicating FTA
Micro Card*
100
1
125
125
WB120208
FTA Gene Card**
100
3
75
225
WB120065
PlantSaver Card
100
4
N/A
N/A
WB120028
CloneSaver Card
5
96
5
480
* Indicating FTA Cards change colour from pink to white when sample is applied and are recommended for use
with clear samples.
**FTA Gene Cards are compatible with automated liquid sampling systems when used with FTA Gene Card Trays.
FTA Reagent, Accessories and Kits
WHATMAN CATALOGUE ORDERING INFORMATION
Catalogue
Number
Description
Qty per
Pack
WB100016
FTA Card Mailer
50
WB100030
FTA Gene Card Tray
20
WB120061
FTA Starter Pack
1
WB120067
FTA Kit
1
WB120204
FTA Purification Reagent
WB100032
Sterile Foam Tipped Applicator Swabs
100
WB100003
Desiccant (1gm)
1000
WB100011
Multi-Barrier Pouch, Small
(for Mini, Micro and Gene Cards)
500
Multi-Barrier Pouch, Large
(for Classic Cards)
500
CloneSaver Resealable
Multi-Barrier Pouch
50
WB120052
CloneSaver Starter Kit
1
WB100005
Harris Micro Punch 1.2mm (with Mat)
1
WB100028
Harris Uni-Core Disposable 1.25mm
Punch (with Mat)
4
WB100007
Harris Micro Punch 2.0mm (with Mat)
1
WB100029
Harris Uni-Core Disposable 2.0mm
Punch (with Mat)
4
WB100020
Replacement Cutting Mat
1
WB100006
Replacement Tip 1.2mm
1
WB100008
Replacement Tip 2.0mm
1
WB100010
WB100024
Whatman is a global leader in separations technology
and is known in the scientific community for
providing innovative Life Science products and solutions.
Our instinct for simplification accelerates the rate
of discovery, reduces costs and saves time. In order
to focus on the unique needs of our customers,
Whatman is organised into four business development
units: Analytical Chemistry, Diagnostics, Genomics &
Proteomics and Medical Devices. For more information,
visit www.whatman.com.
CloneSaver, FTA, PlantSaver and Whatman are registered
trademarks of the Whatman Group.
North America Whatman Inc.
200 Park Avenue
Florham Park, NJ 07932 USA
Technical Support: 1-800-922-0361
Customer Service: 1-800-631-7290
E-mail: [email protected]
Europe Whatman International Ltd
Springfield Mill
James Whatman Way, Maidstone
Kent ME14 2LE UK
Tel: + 44 (0)1622 676670
Fax: + 44 (0)1622 691425
E-mail: [email protected]
Japan Whatman Japan KK
Daiwa Ueno Building 1F
6-10 Ueno 5-chome, Taito-ku
Tokyo 110-0005, Japan
Tel: +81 (0)3 3832 6707
Fax: +81 (0)3 3832 6457
E-mail: [email protected]
500mL
Asia Pacific Whatman Asia Pacific Pte Ltd
171 Chin Swee Road
#08-01 San Centre
Singapore 169877
Tel: +65 6534 0138
Fax: +65 6534 2166
E-mail: [email protected]
Leaders in Separations Technology
www.whatman.com
Cat No. S9036-756
Whatman FTA
®
Collect, archive, transport
and purify nucleic acids
all at room temperature
Whether you’re in a laboratory or deep in a rain
forest, Whatman FTA provides a remarkably easy way
to collect and isolate nucleic acid samples for analysis.
Simply apply virtually any type of biological sample to
the FTA matrix, and the nucleic acids are instantly
captured and stabilised. Pathogens are inactivated,
making samples safe to handle and ship. Store
samples, including clones, at room temperature and
analyse whenever you’re ready. Try FTA, and you’ll
soon find it’s an indispensable part of your nucleic
Three easy steps
to pure nucleic acids
acids toolbox.
Features and Benefits
• Simple collection Protection of
• Fast purification Nucleic acids
nucleic acids from degradation at
are purified on the FTA Card in three
room temperature allows for conven-
simple steps, all in a single tube
ient collection in the laboratory or the
at room temperature. DNA remains
field.
immobilised on the matrix and is
• Room temperature storage Nucleic
acids are automatically stabilised
without the need for refrigeration.
• Pathogen inactivation Cells are
automatically lysed on contact with
the FTA matrix. Pathogens become
inactivated, making samples safe to
handle and ship via standard mail.
bacterial analysis
Punch
ready for PCR or other amplification
techniques.
• Automatable Automation speeds
the handling of multiple FTA punches
and standardises DNA purification.
Punches can be easily washed and
prepared for PCR on a variety of
liquid handling instruments.
Applications
• Blood, plant, insect, viral and
Spot
• Biosafety, food safety and
environmental analysis
• Genetic identification
• HLA typing
• Ideal for clones
• Animal breeding studies
• Diagnostic and clinical applications
• Molecular identification
Purify
FTA – A Highly Flexible Technology used
Widely in a Range of Industries
FTA technology has been embraced by a wide
range of industries across the globe. Pharmaceutical
companies use FTA to collect and archive human DNA
samples for clinical drug trials. Law enforcement
agencies use FTA to collect and archive DNA samples
from convicted offenders. Nature conservationists use
FTA to collect bird DNA from jet engines to determine
the flight patterns of specific species. Scientists
hunting for new plant species use FTA in the field to
collect and safely transport samples. Governmental
agencies use FTA to sample food products while
farmers use FTA to track diseases within multiple herd
generations.
While the range of applications is large, they all share
a common element: simplicity. Whatman FTA helps
scientists speed their research and achieve their goals.
Use with virtually any cell type
FTA DNA PURIFICATION PROTOCOL
The following is a partial list of the cell types that can
be applied to FTA Cards:
• Blood
• Plasmids
• Cultured cells
• Micro-organisms
• Buccal cells
• Solid tissue
• Plant tissue
• Viral particles
• Bacteria
• M13 plaques
FTA Cards are available in either white or pink (Indicating) formats.
Sample Application
Apply specimen and allow to
dry completely.
Disk Removal
Punch a disk out of the sample
area on the FTA Card.
White FTA Cards are recommended for blood samples, plant tissues and other easily identified samples. Indicating FTA Cards are
pink and turn white upon sample addition. Indicating FTA Cards
are recommended for buccal cells, cultured cells and other clear
samples.
Store nucleic acids at
room temperature for years
Genomic DNA stored on FTA Cards at room temperature for more
than 14 years has been successfully amplified by PCR.
No other product can make that claim. FTA Cards offer a
compact room temperature storage system that reduces the need
FTA Purification Reagent Washes
Place the disk in a PCR tube
and wash three times with FTA
Purification Reagent. Discard used
reagent after each wash.
TE-1 Rinses
Wash twice with TE-1 buffer
(10 mM Tris, 0.1 mM EDTA, pH 8.0)
and discard used buffer after
each wash.
Drying Step
Dry disk in PCR tube.
for precious freezer space, improves sample accessibility and
reduces storage costs.
Captured nucleic acid is ready for downstream applications in less than 30 minutes
Captured nucleic acid is ready for purification when you are.
Just take a sample disk from the FTA Card, wash with FTA
Purification Reagent and rinse with TE-1 buffer. The washed disk is
ready to use in applications such as PCR, RFLP analysis and
RT-PCR.
Direct to PCR
Add PCR master mix directly
to the disk and amplify.
FTA Cards, Reagent,
Accessories and Kits
FTA Cards
FTA Cards are available in 1, 2, 3 and 4 part configurations.
Custom configurations are available upon request.
FTA Classic Card
FTA Gene Card
Four sample areas for storage of up to 500µL whole blood or
100µL plant homogenate per card. Convenient for multiple
applications of the same specimen or collection of multiple animal
or plant samples. Also available in Indicating (pink) FTA format.
Three sample areas in a card frame for storage of up to 225µL
whole blood or 30µL plant homogenate per card. Can be run in
most automatic dispensing/pipetting systems when used with the
FTA Gene Card Tray.
FTA Mini Card
CloneSaver™ Card
Two sample areas for storage of up to 250µL whole blood or
50µL plant homogenate per card. Convenient for protocols that
require different locations for testing and archiving samples.
Also available in Indicating (pink) FTA format.
Designed for the collection, storage and purification of plasmid
and BAC DNA from bacterial clones. DNA is stable at room
temperature for at least 5 years (real-time data). Available in a
96 well format for high throughput applications.
FTA Micro Card
PlantSaver™ FTA Card
One sample area for storage of up to 125µL whole blood or 25µL
plant homogenate per card. Recommended when only one sample
is needed. Also available in Indicating (pink) FTA format.
Plant friendly FTA Card, in a Classic Card format. Features a
laminated flap that allows you to vigorously pound the plant
sample into the FTA matrix without damaging the FTA Card.
FTA Reagent, Accessories and Kits
FTA Purification Reagent
Removes heme, PCR inhibitors and other potential contaminants
to ensure superior quality DNA for downstream analysis.
FTA Gene Card Tray
Holds two FTA Gene Cards for use in automatic liquid handling
systems.
FTA Kit
Includes a 25-card supply of FTA Micro Cards; two vials of
purification reagent (25mL); two Harris Uni-Core Punches; a
cutting mat and instructions.
FTA Starter Pack
Provides a sample of FTA products, including one FTA Classic
Card; one FTA Mini Card; one FTA Micro Card; one Indicating
FTA Mini Card and one Indicating FTA Micro Card. Pack also
includes two foam-tipped applicator swabs; one multi-barrier
pouch and desiccant; one vial of purification reagent (25mL); two
Harris Uni-Core Punches; a cutting mat and instructions.
Sterile Foam Tipped Applicator
Easy-to-use applicator for the non-invasive collection and transfer
of buccal cells to FTA Cards.
Harris Micro Punches, Disposable
Uni-Core Punches and Cutting Mat
For the precise sample disk removal from FTA Cards. The 1.2mm
punches are recommended for use with whole blood and samples
with high DNA content. The 2.0mm punches are recommended
for use with buccal cells, plasmids and samples with lower
DNA content.
Multi-Barrier Pouches
For transporting or storing FTA Cards. Protects cards from
environmental contamination. Tamper-evident seal maintains
sample security for forensics samples. A resealable pouch is
also available when multiple access to FTA Cards is needed.
FTA Card Mailer
A rigid protective card mailer for transporting FTA Cards without
biohazard labelling.
Storage Desiccant Packets
Ensure that FTA Cards remain dry during transport or storage.
Contains indicator that changes colour to verify moisture
absorption.
CloneSaver Starter Kit
Includes two CloneSaver Cards; two Harris Uni-Core Punches
(2mm); a cutting mat and instructions.
FTA® Mouse
Tail DNA
Whatman
Raccolta, trasferimento,
stoccaggio e purificazione
del DNA da code di topo
FTA della Whatman offre un valido e semplice metodo per la
preparazione di campioni per analisi di DNA da code di topo.
Occorre, infatti, semplicemente applicare un campione di
sangue o di tessuto dalla coda del topo alla matrice FTA. Il
DNA viene catturato e stabilizzato all’istante, consentendone
lo stoccaggio per un periodo indefinito a temperatura
ambiente, da analizzare in qualsiasi momento. Trasporto
sicuro e purificazione in 30 minuti rendono la tecnologia FTA
un indispensabile strumento di ricerca.
Caratteristiche Principali
• Metodo rapido e versatile di
raccolta I campioni sono depositati
direttamente sulla matrice FTA. Il
DNA è purificato sulla FTA Card in
tre steps semplici, in un singolo
tubo, a temperatura ambiente. Non
sono necessarie sostanze chimiche
tossiche. Il DNA rimane fissato sulla
matrice, pronto per la PCR.
• Adatto a qualsiasi metodo Le
FTA Cards si adattano alle diverse
tipologie di campione :
preparazione di campioni di sangue
o di campioni di tessuto di coda di
topo previo pretrattamento
mediante digestione enzimatica. E’
sufficiente applicare direttamente i
campioni sulla matrice FTA e non è
necessaria alcuna ulteriore
purificazione né con sostanze
tossiche (metodi quali
fenolo/cloroformio) né con altri
metodi che possono richiedere
lunghe incubazioni.
• Stoccaggio a temperatura
ambiente e trasferimento sicuri
Gli acidi nucleici vengono
automaticamente stabilizzati senza
la necessità di congelarli. FTA
inattiva gli agenti patogeni
potenzialmente nocivi rendendo i
campioni sicuri per la loro
manipolazione in laboratorio. Inviare
i campioni a colleghi o al
laboratorio centrale è veramente
facile con FTA Cards. Occorre solo
inviarli per posta!
• Automatizzabile L’utilizzo di
sistemi automatici accelera le fasi di
preparazione di diversi dischi
prelevati contemporaneamente,
ottimizza le risorse disponibili e
standardizza la purificazione di
DNA. I dischi prelevati dalla FTA
Card possono essere sottoposti alle
fasi di lavaggio e preparazione per
la PCR su una diversi tipi di
strumenti presenti in commercio
(liquid handling robot).
Applicazioni
• Screening di topi transgenici
• PCR
• Identificazione genetica
• Analisi SNP
• Studi di allevamento
• Whole genome amplification
• Applicazioni diagnostiche
Tre semplici passaggi operativi
per ottenere un DNA puro
Dispensare il campione
Prelevare un
piccolo disco
(punch) dal
Campione
Purificare
PROTOCOLLO APPLICATIVO DEL FTA MOUSE TAIL DNA
Dispensare il campione
Tagliare approssimativamente
1,5cm di coda del topo,
stringere gentilmente perchè
il sangue venga alla
superficie del taglio,
appoggiare leggermente la
FTA Card sul sangue. Oppure
applicare la raccolta
direttamente sull’FTA Card.
Lasciare asciugare
completamente.
Lavaggio con soluzione
tampone TE-1
Effettuare due lavaggi con
la soluzione tampone TE-1
(10mM Tris, 0,1 mM EDTA,
pH 8,0). Eliminare la
soluzione usata dopo
ciascun lavaggio.
Essiccazione
Lasciar asciugare il disco nel
tubo PCR.
Prelevare un piccolo disco
Prelevare (mediante
perforazione) un piccolo
disco dal campione
depositato sulla FTA Card.
Purificazione con
Reagente di Purificazione
FTA (Purification Reagent
FTA)
Mettere il disco di campione
in un tubo per PCR ed
effettuare tre lavaggi con il
Reagente di Purificazione
FTA. Eliminare la soluzione
utilizzata dopo ciascun
lavaggio.
PCR
Aggiungere la master mix
per la PCR direttamente al
disco e iniziare la reazione
di amplificazione.
Whatman, azienda leader nelle tecnologie per la separazione,
è nota nellà comunità scientifica per prodotti e soluzioni
innovative contraddistinte da un’elevata qualità. Il nostro
istinto per la semplificazione accelera la velocità di fare nuove
scoperte, riduce i costi e risparmia tempo. Inoltre, per
accentuare ulteriormente l’impegno nel soddisfare le
specifiche esigenze dei nostri clienti, Whatman è organizzata
in quattro aree distinte: LabScience, BioScience, MedTech
Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono
disponibili collegandovi al sito www.whatman.com.
FTA, GenSpin e Whatman sono marchi registrati della
Whatman Group.
INFORMAZIONI PER L’ORDINE DAL CATALOGO WHATMAN
No. Cat.
Descrizione
Quantità
per Confezione
WB120061
WB120204
WB120205
WB120055
WB120210
WB120208
WB100007
WB100008
WB100029
WB100010
WB100011
WB120216
WB120217
WB100026
WB100003
WB100016
WB100020
WB100030
WB120005
FTA Starter Pack
FTA Reagente di Purificazione
FTA Classic Card (non-indicatrice)
FTA Mini Card (non-indicatrice)
FTA Micro Card (non-indicatrice)
FTA Gene Card
Harris Micro Perforatore 2.0mm
Harris Micro Perforatore 2.0mm Tip
Harris Uni-Core 2.0mm Perforatore
Busta Multi-Barrier (grande)
Busta Multi-Barrier (piccola)
FTA Gene Card/Busta/Essiccante
FTA Classic Card/Busta/Essiccante
2.0mm Plunger di Ricambio
Essiccante (1g)
Busta Postale per FTA Card
Tagliere di Ricambio
Supporto per FTA Gene Card
GenSpin Kit di Purificazione DNA
1
500mL
100
100
100
100
1
1
4
500
500
1000
1000
1
1000
50
1
20
50 Purificazioni
Purificazione del DNA in meno di 25
minuti! Il GenSpin Kit di Purificazione
del DNA della Whatman rappresenta un
metodo estremamente semplice per
purificare il DNA a singolo filamento da
campioni di sangue e cellule in coltura. Il
campione è subito pronto per la PCR a
partire anche da piccoli volumi del
campione di partenza.
GenSpinTM Kit di Purificazione DNA Whatman
Qualità Whatman
North America Whatman Inc
9 Bridewell Place,
Clifton, NJ 07014
Technical Support: 1-800-922-0361
Customer Service: 1-800-631-7290
Outside US: 973-773-5800
Fax: 973-773-0168
E-mail: [email protected]
Europe Whatman International Ltd
Springfield Mill
James Whatman Way
Maidstone
Kent ME14 2LE, UK
Tel: +44 (0)1622 676670
Fax: +44 (0)1622 691425
E-mail: [email protected]
Japan Whatman Japan KK
Daiwa Ueno Building 1F
6-10 Ueno 5-chome, Taito-ku
Tokyo 110-0005, Japan
Tel: +81 (0)3 3832 6707
Fax: +81 (0)3 3832 6457
E-mail: [email protected]
Asia Pacific Whatman Asia Pacific Pte Ltd
171 Chin Swee Road
#08-01 San Centre
Singapore 169877
Tel: +65 6534 0138
Fax: +65 6534 2166
E-mail: [email protected]
Leaders in Separations Technology
www.whatman.com
Cat No. S9036-783
FTA® Plant
DNA Whatman
Raccolta, trasferimento
e purificazione del DNA
di Piante
In laboratorio o in mezzo ad una foresta, FTA della
Whatman offre un valido e semplice metodo per la
preparazione di campioni per analisi di DNA di piante.
Occorre, infatti, semplicemente applicare sulla matrice
FTA il campione vegetale tal quale o dopo averne
ottenuto un omogenato. Il DNA viene catturato e
stabilizzato all’istante, consentendone lo stoccaggio
per un periodo indefinito a temperatura ambiente, da
analizzare in qualsiasi momento. Trasporto sicuro e
purificazione in 30 minuti rendono la tecnologia FTA
un indispensabile strumento di ricerca.
Caratteristiche Principali
• Raccolta semplice di campioni sul
campo La stabilità degli acidi
nucleici a temperatura ambiente e la
protezione dalla degradazione
rendono la tecnologia FTA uno
strumento ideale per la raccolta di
campioni sul campo.
• Meno campione E’ possible
utilizzare anche solo le foglie più
giovani, riducendo il tempo di
crescita necessario e accelerando la
ricerca.
• Stoccaggio a temperatura
ambiente e trasferimento sicuri
Consente di raccogliere ovunque il
DNA dalle piante, di trasportarlo al
laboratorio e purificarlo solo quando
necessario.
• Purificazione rapida Il DNA viene
purificato sulla FTA Card in tre
semplici steps, in un singolo tubo, a
temperatura ambiente. Non sono
necessarie sostanze chimiche
tossiche, inoltre rimanendo il DNA
fissato sulla matrice è subito pronto
per la PCR. Se il DNA è richiesto in
soluzione, usare GenSpin Plant la
Whatman.
• Ideale per automatizzazione
L’utilizzo di sistemi automatizzati
accelera le fasi di manipolazione di
multiple FTA Cards, riducendo i
tempi di lavoro e standardizzando la
purificazione del DNA. I dischi
prelevati dalla FTA Card possono
essere sottoposti alle fasi di lavaggio
e preparazione per la PCR su diverse
tipologie di strumenti presenti in
commercio (liquid handling robot).
Applicazioni
• Analisi del DNA di piante
mediante saggi PCR
• Selezione di colture mediante
marker genetici
• Identificazione delle varietà
vegetali
• Analisi di filogenesi
• Amplificazione di loci genetici
LCN (low copy number loci)
• Invader assay
• Multiple displacement
amplification
• Identificazione di specie
transgeniche
Tre semplici passaggi
operativi per ottenere puro
DNA da piante
Depositare Campione
Perforare
un Disco
di
Campione
Purificare
PROTOCOLLO APPLICATIVO DEL FTA PLANT DNA
Dispensare il campione
Depositare il campione
vegetale o l’omogenato
sulla FTA Card. Lasciarlo
asciugare completamente.
Soluzione tampone TE-1
Lavare due volte con soluzione
tampone TE-1 (10mM Tris, 0,1
mM EDTA, pH 8,0). Eliminare la
soluzione tampone usata dopo
ciascun lavaggio.
Prelevare un piccolo disco
(punch) dal Campione
Prelevare mediànte
perforazione un disco dalla
matrice FTA impregnata di
materiale vegetale.
Essiccazione
Lasciar asciugare il disco nel tubo
PCR
Purificazione con
Reagente di Purificazione
FTA (Purification Reagent
FTA)
Mettere il disco di campione
in un tubo per PCR ed
effettuare tre lavaggi con il
Reagente di Purificazione
FTA. Eliminare la soluzione
utilizzata dopo ciascun
lavaggio.
PCR
Aggiungere la master mix per la
PCR direttamente al disco e
iniziare la reazione di
amplificazione.
Qualità Whatman
Whatman, azienda leader nelle tecnologie per la separazione,
è nota nellà comunità scientifica per prodotti e soluzioni
innovative contraddistinte da un’elevata qualità. Il nostro
istinto per la semplificazione accelera la velocità di fare nuove
scoperte, riduce i costi e risparmia tempo. Inoltre, per
accentuare ulteriormente l’impegno nel soddisfare le
specifiche esigenze dei nostri clienti, Whatman è organizzata
in quattro aree distinte: LabScience, BioScience, MedTech
Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono
disponibili collegandovi al sito www.whatman.com.
FTA, GenSpin e Whatman sono marchi registrati della
Whatman Group.
INFORMAZIONI PER L’ORDINE DAL CATALOGO WHATMAN
No. Cat.
Descrizione
Quantità
per Confezione
WB120061
WB120204
WB120205
WB120055
WB120210
WB120208
WB100005
WB100006
WB100028
WB100010
WB100011
WB120216
WB120217
WB100025
WB100003
WB100016
WB100020
WB100030
WB120046
SWB120046
FTA Starter Pack
FTA Reagente di Purificazione
FTA Classic Card (non-indicatrice)
FTA Mini Card (non-indicatrice)
FTA Micro Card (non-indicatrice)
FTA Gene Card
Harris Micro Perforatore 1.2mm
Harris Micro Perforatore 1.2mm Tip
Harris Uni-Core 1.25mm Perforatore
Busta Multi-Barrier (grande)
Busta Multi-Barrier (piccola)
FTA Gene Card/Busta/Essiccante
FTA Classic Card/Busta/Essiccante
1.2mm Plunger di Ricambio
Essiccante (1g)
Busta Postale FTA Card
Tagliere di Ricambio
Supporto per FTA Gene Card
GenSpin Plant Kit
GenSpin Plant Kit di Prova
1
500mL
100
100
100
100
1
1
4
500
500
1000
1000
1
1000
50
1
20
50 Purificazioni
5 Purificatzioni
Avete bisogno di DNA in soluzione?
Questo kit di semplice utilizzo è ideale
per la rapida preparazione di DNA a
doppio filamento da piccole quantità di
materiale vegetale, per analisi PCR. Il
materiale raccolto dalle piante può essere
omogeneizzato a temperatura ambiente
e purificato in meno di 30 minuti.
GenSpin™ Plant Kit di Purificazione DNA Whatman
North America Whatman Inc
9 Bridewell Place,
Clifton, NJ 07014
Technical Support: 1-800-922-0361
Customer Service: 1-800-631-7290
Outside US: 973-773-5800
Fax: 973-773-0168
E-mail: [email protected]
Europe Whatman International Ltd
Springfield Mill
James Whatman Way
Maidstone
Kent ME14 2LE, UK
Tel: +44 (0)1622 676670
Fax: +44 (0)1622 691425
E-mail: [email protected]
Japan Whatman Japan KK
Daiwa Ueno Building 1F
6-10 Ueno 5-chome, Taito-ku
Tokyo 110-0005, Japan
Tel: +81 (0)3 3832 6707
Fax: +81 (0)3 3832 6457
E-mail: [email protected]
Asia Pacific Whatman Asia Pacific Pte Ltd
171 Chin Swee Road
#08-01 San Centre
Singapore 169877
Tel: +65 6534 0138
Fax: +65 6534 2166
E-mail: [email protected]
Leaders in Separations Technology
www.whatman.com
Cat No. S9036-784
FTA
Author
Aranda et al.
Aranda et al.
Aranda et al.
Babu
Title
FTA Technology, Unique Formats for
Year
Publication
2001 Poster: Promega 12th
the Collection, Shipment, Archiving
International Symposium on
and Processing of Biological Samples
Human ID
A Simple Device for the Efficient
2003 Poster: Promega 14th
Transfer of Buccal Swab Cells onto
International Symposium on
FTA® Paper
Human ID, Phoenix AZ
Alkaline Extraction of DNA from
2004 Poster: Promega 15th
FTA Paper Spotted with Buccal
International Symposium on
Epithelial Cells and Whole Blood
Human ID, Phoenix AZ
Standing Orders - Institute of
Misidentification on the Estimation
Two Novel Missense Mutations of
Swada-Hirai, BE the HFE Gene (I105T and G93R) and
Rothenberg and Identification of the S65C Mutation
RT Acton
on Alabama Hemochromatosis
Probands.
forensic, databasing,
human id
Alkaline conditions were used to extract DNA from FTA
DNA in solution, real time
cards with blood or buccal cells applied to them. Extracted PCR, extracted DNA
DNA was quantitated using the real time PCR kit
Quantifiler from ABI. For blood samples extracted DNA
from a 3mm punch ranged from 74 to 206 ng/punch; 7mm
punches ranged from 165ng to 1.57ug. For buccal
samples extracted DNA from a 3mm punch ranged from 35
- 52ng/punch; 7mm punches ranged from 31-109ng/punch.
2002 Genetics and Molecular Biol
FTA used to collect blood samples from cattle. 2mm
punches were washed and the DNA amplified by PCR in
40 ml reactions directly. Also DNA from 6mm FTA punches
were extracted using Chelex.
25(4):389-394.
of Breeding Value in Gir Cattle.
Barton, JC, R
Describe how the SAMPACT device works. SAMPACT is
a plastic cassett containing an FTA card that induces and
equal pressure on a foam swab to get even transfer of
buccal cells onto the FTA card
Frame work as to how to administer and maintain aircrew's DNA repository, military,
DNA repository for use in identification following an aircraft air force, disaster victim
accident. How and when to retrieve archived samples.
identification, blood
samples, storage
conditions, human id
Repository
Parentage Testing and Effect of
Keywords
DNA elution, hair root,
buccal, human, blood,
restriction digest DNA
from FTA, hair digestion,
STR, extract DNA,
microsatellites, human id,
forensics
2002 Royal Australian Air Force
Aviation Medicine Aircrew DNA
Baron et al.
Comment/Summary
Demonstrates FTA for downstream processes such as
multiplex PCR for human identification. Sample types
include hair roots, buccal cells, and blood
1999 Blood Cells, Molecules and
Diseases 2 5(9)146-154.
PCR, microsatellites,
cattle, blood, parentage,
genetic identity, breeding,
dairy cows, Gir, genetic
value of bulls, animal
biotechnology
Note: a proband is a person with hemochromatosis a
human, population study,
disease where there is too much iron in the blood, giving
blood disease, saliva,
blood is a "treatment". Genomic DNA from saliva samples buccal cell,
collected from volunteers on FTA for this study. Regions of
the HFE gene were amplified by PCR then analyzed by
sequencing and dot-blot hybridizations.
FTA Reference DatabaseJun05
1
Beck et al.
Simple, Sensitive, and Specific
Detection of Human
2001 Journal Clinical Microbiology
39: 29-33
Easy collection in the field for HIV testing in a central lab.
Depicts High Sensitivity.
Immunodeficiency Virus Type 1
Subtype B DNA in Dried Blood
archiving, human, blood,
PCR, HIV, sensitivity,
specific, diagnosis,
infection, pol, dried blood
spots, diagnostics
Samples for the Diagnosis in Infants
in the Field
Beck and
Genotyping Kits for the Detection of 2003 NIH AIDS Research and
Frenkel
HIV-1 pol Drug Resistance
Reference Reagent Program
Mutations by an Oligonucleotide
Ligation Assay
Becker et al.
Real-time PCR for detecting
Trypanosoma brucei in human blood
2004 Diag. Microbiol. Inf. Dis.
50:193-199.
samples
Belgrader &
Automated Sample Processing Using
Marino
Robotics for Genetic Typing of STR
1997 Lab. Robotics & Automation
9: 3-7
Instructions for a kit to analyze HIV pol DNA. Whole blood point mutation, PCR,
collected on FTA (pg 14) can be used in the
HIV, pol gene,
oligonucleotide ligation assay (OLA). These instructions
diagnostics
also provide a good overview as to what the OLA is; briefly
OLA is a way to identify a point mutation in a gene. A
segment of the DNA is amplified by PCR then 2
oligonucleotides are hybridized to the PCR product. The 2
oligos are then ligated with a ligase enzyme. Only when a
specific mutation is present will the oligos ligate and are
detected.
Blood spiked with dilutions of cultured T. brucei and blood pathogens, disease
from patients with sleeping sickness were spotted to FTA diagnostics, human,
bacteria, real-time PCR
cards. Punches of 2.0mm were washed according to the
standard protocol then the DNA extracted using Chelex
100 resin. For real time PCR 4µl of the Chelex
supernatant were included in the 10µl PCR mix.
Use of FTA to rapidly produce template suitable for
genotyping ID on an automated platform.
Polymorphisms by Capillary
Electrophoresis
Belgrader et al. Automated DNA Purification and
1995 BioTechniques 19: 427-432
Blood sample processing on FTA using an automated
(Beckman) platform..
robotic, Biomek 1000,
blood, human,
automation
1997 Poster 8th International
The isolation of DNA using FTA & Rosys robotic
workstation.
human, blood,
PowerPlex, DNA Plate,
human id, forensics,
automation
FTA used to collect blood samples from drug using
volunteers in an HIV study.
HIV, genetic diversity,
infection, virus,
diagnostics, population
study
Amplification from Bloodstain Cards
Using a Robotic Workstation
Bever et al.
Implementation of Laboratory
Automation for the analysis of STR
Symposium on Human ID,
loci
Beyrer et al.
Molecular Epidemiology of HIV-1
robotic, automation,
PCR, high-throughput,
HT, blood, human, 96well, human id, forensics,
automation
2003 Poster 10th Conference on
Among Northern Thai Drug Users:
Retroviruses and
Implications for Vaccine Efficacy
Opportunistic Infections
Trials.
FTA Reference DatabaseJun05
2
Bhattacharya et Use of Reverse Transcription and
al.
PCR to Discriminate Between
2004 J Virological Meth. 116:181187.
Infectious and Non-Infectious
Hepatitis A Virus.
Bilyeu KD and
Genetic Divergence between North
Beuselinck PR
American Ancestral Soybean Lines
2005 Journal of Heredity doi:
Study was to examine genetic diversity in soybean
genotypes present in USDA collection using SSRs in the
chloroplast genome. Leaf tissue was pressed onto FTA
cards or DNA from leaf tissue was prepared using Qiagen
Plant mini kit or Promega Wizard Magnetic 96 Plant kit. 1
FTA punch or 10 - 100ng of DNA was included in each
PCR amplification.
MultiPROBE® II Forensic workstation used to spot whole
blood to FTA Gene Card in Gene Card tray for long term
archiving. Shows how FTA and automation fit into forensic
lab work flow.
Soybean, germplasm
collection, chloroplast
DNA,
2003 Croat Med J 44:322-326.
FTA used to collect blood samples from relatives of
missing persons for genomic DNA extraction.
human, missing persons,
ID, STR, PCR, skeleton,
genetic match, chelex,
genotyping, databasing,
human id, forensics
2004 Am. J. Trop. Med. Hyg.
Blood collected from study participants in vacutainer tubes
with some being spotted onto FTA for genomic DNA
analysis of bacteria from the genus Rickettsia . A nested
PCR method was used to identify a Rickettsia specific
gene, htrA .
field collection, human,
blood, bacterial
infections, environmental,
diagnostic, population
study
Final PCR volume of 5µl gives good, reproducible, STR
profiling results; cycles reduced form 25 to 23.
blood, buccal, human,
RCMP, STR, PCR,
forensics, human id
10.1093/hered/esi087
and Introductions with Resistance to
Soybean Cyst Nematode Revealed by
Chloroplast Haplotype
Biondi et al.
Forensic Sample Processing using a
Hepatitis A virus (an RNA virus), released into cell culture RT-PCR, RNA, virus,
media from infected cells was applied to FTA cards. The environmental, food
RNA was eluted from the cards in Tris-EDTA buffer
pathogen, food safety,
containing 2-mercaptoethanol then preciptated with
isopropanol in the presence of carrier tRNA. RNA was also
prepared using Trizol. Detection was more sensitive for
RNA prepared on FTA than with Trizol.
2003 Poster Promega 14th
Robotic Workstation: Automated
International Symposium on
Paper-Based Spotting of Whole Blood
Human Identification
Gene Card, blood,
automaton, forensic,
human ID
convicted Offender Samples and
High Throughput DNA Isolation for
STR Analysis.
Birus et al.
How High Should Paternity Index Be
for Reliable Identification of War
Victims by DNA Typing?
Blair et al.
Evidence of Rickettsial and
Leptospira Infections in Andean
70(4): 357-363.
Northern Peru
Borys et al.
PCR Volume Reduction Study Using
Bloodstained FTA Collection Cards
1998 Poster Promega 9th Annual
Int Symp Of Human ID.
and Capillary Electrophoresis
Borys. S
Evaluation of High Throughput STR
1999 MSc. Thesis Ottowa-Carleton Use of FTA to collect blood, buccal cells for DNA
Technologies for Potential
Institute of Biology, Carleton analysis and databasing. Reducing PCR from 25 ml to 5
Implementation in the National DNA
University, Ottowa Canada
ml increases sensitivity 9-fold.
Databank
FTA Reference DatabaseJun05
DNA bank, repositories,
database, DNA typing,
genotyping, human,
blood, buccal, databank,
forensics, human id
3
Bosman et al.
Reverse Line Blot: A diagnostic tool
to detect blood parasites.
2002 Presentation: 31st Annual
Blood from various animal species was applied to FTA or
collected in vacutainers with various anti-coagulants. DNA
Conference of the
was extracted or FTA punches prepared with the standard
Parasitological Society of
procedure and analyzed by PCR for the presence of
Southern Africa (abstract # Babesia or Theileria parasites.
Parasites, whole blood,
animal, PCR, animal
biotechnology
17)
Both et al.
FTA Paper, DNA, Time, and the
Profiler
2000 Web Article from Forensic
Science Center Adelaide,
Australia
Review of FTA for the collection & archiving of
DNA typing, blood,
buccal, human, archive,
offender blood samples….. RT storage for 9 years w/out
PCR, STR, integrity,
signal loss. Punches from blood stained FTA could be
human id, forensics
PCR'd without washing, but punches containing buccal
cells required washing for successful PCR. ABI
ProfilerPlus PCR kit was used in this study.
Budowle et al.
DNA Typing Protocols: Molecular
Biology and Forensic Analysis.
2000 BioTechniques® Books
Publication, Eaton Publishing,
Natick MA
Description of FTA as a sample collection medium for
genomic DNA, forensic,
DNA typing, PCR, CEP
swab, human id,
punch for RFLP or PCR-based analysis. Shows photos of forensics
CEP swab aka OmniSwab and Gene Guard Swab aka
blood and buccal cells. Protocol for preparing FTA
Foam tipped applicator for collecting buccal cells.
Burgoyne
Convenient DNA Collection and
1997 Poster Promega 8th
Processing: Disposable Toothbrushes
International Symposium on
and FTA® Paper as a Non-
Human ID
Use of a cytobrush to collect buccal cells to deposit on FTA buccal cell, human,
buccalbrush, food
coloring, forensics,
human id
threatening Buccal-Cell Collection Kit
Compatible with Automatable DNA
Burgoyne &
Processing
Studies with FTA
Hallsworth
1995 Presentation 5th
International Symposium on
Human ID
Castilho et al.
DNA Template Preparation and
Archiving for Blood Group
Genotyping of Remotely Located
FTA kills HSV within 5 sec. FTA prevents fungal growth HSV, Herpes Simplex
Virus, 903, 3MM,
on blood spots held at body temp and high humidity.
FTA923,
FTA can be used to detect HSV, CMV (DNA viruses)
Cytomegalovirus, CMV,
and Hepatitis C (RNA virus) and protect samples from Hepatitis C, DNA virus,
RNA virus, diagnostics
accelerated and natural aging.
2001 Poster: American Association FTA cards were used for blood group genotyping using
whole blood, plasma, and amniotic fluid. The cards were
of Blood Banks, San Antonio
used for collection in remote areas and shipped back to
2001
central lab for testing.
storage, transportation,
human, PCR, RFLP,
population study, human
id, diagnostics
Patient Populations
FTA Reference DatabaseJun05
4
Castilho et al.
Genotyping for DO A/DO B
Facilitates Transfusion of Sickle Cell
Disease Patients
Cerda-Flores et Maximum Likelihood Estimates of
al.
2001 Poster: American Association Blood samples from patients who received a transfusion Dombrock gene, RFLP,
human, blood,
of Blood Banks, San Antonio was spotted to FTA, shipped back to central lab for
genotyping, population
2001
PCR analysis.
study, human id,
diagnostics
2002 Am J Hum Biol 14:429-439.
Blood from people in Northeastern Mexico were
Admixture in Northeastern Mexico
collected and FTA and analyzed by PCR for 13 STRs.
Using 13 Short Tandem Repeat Loci.
Data was analyzed to determine lineage contributions
PCR, 1 mm punch, STR,
human, blood, population
study, human id
from Europe, American Indian and African origin.
Chappuis et al.
Options for Field Diagnosis of Human 2005 Clin. Microbiol. Rev. 18(1)133- FTA is described for collecting blood, lymph node fluid
African Trypanosomiasis
146.
or CSF for detection of trypanosome DNA. FTA is
preferred over untreated filter paper because FTA
protozoa, protozoan,
microorganism, disease
diagnostics, PCR,
protects DNA from degradation.
Chu et al.
Survey of Nepalese Animals for the
Presence of Cyclospora Cayetanensis
Chu et al.
Detection of Cyclospora
2003 Poster: Am Assoc.
FTA used to collect animal fecal samples to detect
Microbiology, Washington DC protozoan parasites
2004 Am. J. Trop. Med. Hyg
disease diagnostics, fecal
samples, animals,
detection, PCR, nested
PCR
base polymerase chain reaction
FTA was used to collect fecal samples of dogs,chickens disease diagnostics, fecal
samples, animals,
and monkeys to detect protozoan parasites capable of
detection, PCR, nested
being transmitted to humans and causing diarrheal
PCR
illnesses. Fecal samples were collected in 2.5%
method
potassium dichromate (to stabilize the protozoan
Cayetanensis in animal fecal isolates
71(4):373-379
from Nepal using and FTA filter-
oocytes). Stool specimens 10 - 20ul were spotted
directly onto FTA and dried on a hot block at 56oC. The
disks were washed as usual then amplified.
Connolly et al.
Automated methods for processing
samples stored on FTA® paper
1997 Poster 11th International
Symposium on Human ID,
Biloxi, LA
Connolly et al.
D12 archiving & recovery of bacterial 1999 Poster 13th International
plasmids using FTA paper
Mouse genome Conference
FTA has proven itself to be a robust & versatile method for plate washers,
collection, storage & analysis of DNA. With (semi-)
throughput, automation
automated methods is capable of high- throughput
analysis.
Archiving & recovery of clones ranging from 2Kb to 227Kb. plasmid DNA, freezer,
storage, CloneSaver, 96well, M13 sequencing,
genomics
FTA Reference DatabaseJun05
5
Crabbe
A novel method for the transport
and analysis of genetic material from
2003 J. Biochem. Biophys.
Methods 57: 171-176
polyps and zooxanthellae of
scleractinian corals.
Davis et al.
DNA Tests in Prolific Sheep from
Eight Countries Provide New
2002 Biology of Reproduction
66:1869-1874.
Evidence on Origin of the Booroola
(FecB) Mutation.
Davis, et al.
Del Rio
Del Rio et al.
A Novel Genvault Multiwell Plate
2003 Poster: Society for
Format for Whatman FTA in Robotic,
BioMolecular Screening
High Throughput DNA Archiving
Meeting
Cost Effectiveness in Sample
2001 Poster from Promega 8th
Processing using the FTA Treated
International Symposium on
Stain Card for High Throughput
Human Identification
Reusing the Same Blood-Stained
1996 BioTechniques 20: 970-974
Both the polyps (the soft living part of coral, about the size
of a pencil eraser) and the symbiotic algae (zooxanthellae)
that are part of coral colonies were collected and put on
FTA. Fragments were ground and applied to FTA. DNA
was extracted using Qiagen DNeasy. Ribosomal genes
were PCR amplified for species identification. FTA
excellent for field collection and transport of samples from
tropical locations.
coral, algae,
identification, ribosomal
genes, extracted DNA,
tropical, field collection,
room temperature
transport, plant
Blood samples from sheep collected onto FTA for DNA
animal biotechnology,
preparation. FTA disks (1.2mm) were amplified by PCR
blood, mutation analysis
and the amplicons subjected to RFLP analysis. PCR
amplicons prepared from FTA disks containing sheep DNA
were also subjected to TaqMan Assays.
Describes the Genvault system and the EasyClone 384
well plate
EasyClone 384 well
Important for showing the cost-effectiveness of FTA for
blood collection and processing compared to vacutainer
collection.
offender specimens,
archive, collection, DNA
database, transport,
sample tracking, human,
blood, buccal, human id,
forensics
Demonstrates that not all NA bound to FTA cannot be
human, genotyping,
databanks, human id,
diagnostics, forensics,
research
Punch for Sequential DNA
removed with heat, and punches can be re-used for
Amplifications and Typing
many different PCR amplifications multiple times (4
Plate
sequential runs)
Del Rio, S.
Del Rio-
Amplification of DNA from Bone
1997 Application Note from Fitzco Different sample types than blood processed
Marrow Aspirate and Cervical
successfully on FTA (either spotted or brushed on the
Smears on FTA Blood Stain Cards
cards)
New Method for Collection and
LaFreniere et al Detection of K-ras Point Mutations
1998 Poster: AMP Meeting
Washington DC
Samples of pancreatic tissue, ductal brushings, cells,
diagnostic tests,
diagnostics,
cancer research, tissue
fine needle aspirates or fluid were applied to FTA. FTA screening for mutation,
from Tissue Specimens for Clinical
cards were pressed to tissue samples. A 3mm punch
Diagnostic Applications
was taken and washed as normal. PCR master mix was
diagnostics
added to the washed punch to detect mutation in the K-
ras gene. Bile and fine needle aspirates showed the
weakest signal on FTA but the others gave good PCR
bands on gels.
FTA Reference DatabaseJun05
6
Del Rio-
A Unique Method of Detection of
LaFreniere SA,
the Prothrombin 20210A (FactorII)
Conference on DNA
3mm punches subjected to a specific PCR amplification
McGlennen RC
Mutation Using the Simultaneous
Technologies in Human
procedure for detecting a mutation in the prothrombin
Allele Specific Amplification (SASA)
Detection
gene.
Devost & Choy
Mutation Analysis of Gaucher
Disease Using Dot-Blood Samples on
1998 Poster: 13th San Diego
2000 American Journal of Medical
Genetics 94: 417-420
FTA Filter Paper
Dobbs et al.
Use of FTA Gene Guard Filter Paper
for the Storage and Transportation
of Tumor Cells for Molecular Testing
Dutton and
A Modified Protocol for Sex
Tieber
Identification of In Ovo Avian
Embryos and its Application as a
Human Blood sample were collected on FTA and washed disease diagnostics,
PCR, mutation research,
Polymorphism detection of alleles implicated in Gaucher diagnostic tests, RFLP,
mutation, genotyping,
Disease on population bloods collected & stored on FTA.
nested PCR, human,
diagnostics, population
study
2002 Archives Pathology and
Shows FTA for tumor cell collection, room temperature
pathology, cancer, lymph
storage, transport, and DNA testing. Compares FTA with a node, cell suspensions,
Laboratory Medicine 126: 56Qiagen method.
lung, breast,
63
endometrium, ovary,
kidney, thyroid, research,
DNA banking
blood, animal
2001 J Zoo Wildlife Med 32(2)176- Small amounts of blood were collected by syringe from
blood vessels in developing bird eggs. The blood/fluid was biotechnology, birds,
180.
spotted to FTA and analyzed to determine the gender of
chicken, pigeon, owl
the embryo.
Management Tool for Endangered
Species Conservation Programs.
Drescher &
PCR-genotyping of barley seedlings
Graner
using DNA samples from tissue
2001 Plant Breeding 121, 228-231
prints
Dyer et al.
Detection of Bacilli Spores Using PCR 2003 Poster 103rd General
and FTA Filters
Meeting American Society
Method to sample barley leaves for PCR analysis using
FTA. Compares FTA to CTAB standard method; achieve
comparable results quicker and easier.
marker assisted
breeding, plant,
screening, leaves, high
throughput, genotyping,
field collection
FTA used to isolate DNA from Bacilli spores for nested
PCR detection.
spores, Bacillus, food,
clinical, environmental,
for Microbiology
Elliot
Isolation of DNA Using FTA Blood
Stain Collection Cards
1998 Poster 7th Annual
International Symposium of
Human ID
FTA for forensic collection, archive, rapid purification
RCMP, rapid collection,
rapid extraction, human,
tool. 9 months RT archiving. Did not let card dry after
saliva, buccal, STR,
blood application, put it wet into pouch with desiccant
blood, storage conditions,
forensics, human id
without effecting PCR.
FTA Reference DatabaseJun05
7
Elliot et al.
Extraction of DNA from FTA®
1997 Poster 8th International
Blood Stain Collection Cards for
Symposium on Human ID,
Construction of a Large STR National
FTA tested for DNA stability, yield (peak height on a gel)
ease of use and consistency with blood fresh, frozen,
saliva and semen. Did not have good luck with purified
DNA on FTA with standard wash protocol.
DNA Data Base
Expert et al.
Evaluation of Two Techniques for
Extraction and Conservation of DNA
of Various Quarantine Bacteria
Fici et al.
Sequence Based Typing of Blood
Spots on Filter Paper After
2004 Poster EPPO Conference on
Extraction of DNA from plant disease bacteria by FTA
compared to Q Biogene kit. Both methods able to detect
Quality of Diagnosis and New
gram (-) bacteria at low levels seen in plants. Both
Diagnostic Methods for Plant methods could only detect gram (+) bacteria at higher
Pests. Noordwijkerhout, NL concentrations.
1998 Poster Human Immunology
59(1) 141
Blood and lymphocyte samples spotted to FTA cards
then processed for HLA & blood typing after long-term
Extended Storage
storage. PCR from FTA paper more robust than PCR
from untreated filter paper. A treatment with
RCMP, rapid collection,
rapid extraction, human,
saliva, buccal, STR,
blood, storage conditions,
forensics, human id
plant disease, microbes,
untreated filters, spin
basket, 30 months, 2.5
years storage, Proteinase
K, diagnostics, population
study
Proteinase K (5 ml 10 mg/ml proK added to 495 ml FTA
purification reagent containing punch; incubate 1 hr @
60oC)was required to amplify one class of the HLA.
Forrest et al.
Polymorphism at the Ovine b3-
2003 Animal Genetics 34:19-25.
FTA used to collect blood from lambs. DNA on FTA
Adrenergic Receptor Locus:
punches were examined via PCR for differences in the
Associations with Birth Weight,
sequence in the b3-adrenergic receptor gene which
Growth Rate, Carcass Composition
were then compared to phenotypic traits of the sheep.
PCR-SSCP,
polymorphisms, body
weight, marker assisted
breeding, animal ID,
animal biotechnology
and Cold Survival.
Forrest et al.
Two Rare Polymorphisms in the
2004 Croat Med J 45:457-460
Method to Mitigate Their Impact on
Blood collected onto FTA and used in human genotyping human id, forensics, DNA
polymorphisms, DNA
with the commercially available kits. The researchers
sequencing, STR
saw a new peak in two of the loci, sequenced the DNA
Human Identification
and found DNA base changes that affected the STR
D8S1179 and D13S317Markers and
profile. They make suggestions as to what that means
for identifying humans in forensic cases.
Fox et al.
RT-PCR from Eukaryotic Cells Stored 2000 Poster Abstract: Plant and
on FTA Archival Paper
Animal Genome VIII San
Diego
Demonstrates FTA for collection, storage, processing of
mRNA for RT-PCR- mRNA from human cells spotted onto
FTA was stable for 1 -2 mos room temp or > 10 mos at 20oC or -70oC.
FTA Reference DatabaseJun05
RNA extraction, plant,
BHK21 cells, HeLa cells,
high copy number mRNA,
low copy number,
8
Franchina et al. Polymorphism of the CD30 Promoter
Microsatellite Repressive Element is
2005 Cancer Epidemiol Biomarkers
Prev 14(5) 1322-1325.
Blood from patients was collected on FTA for PCR
amplification of sections of the CD30 gene.
population study, cancer
research
Associated with Development of
Primary Cutaneous
Fujiwara et al.
Lymphoproliferative Disorders.
Plasma DNA Microsattelites as
1999 Cancer Res. 59:1567-1571.
Blood collected from 76 cancer patients and 20 healthy
donor volunteers and spotted to FTA for genomic DNA
extractions and long term storage.
cancer research, tumor
progression, Loss of
heterozygosity, human,
diagnostic
2003 ICPTV Newsletter 8 pg 25-
FITCA is an EU funded project to control tsetse infestation
in Uganda. Blood from cattle (300 - 400) ear veins was
collected directly onto FTA cards and transported back to
the lab for analysis by PCR for 3 species of Trypanosome.
Detection of parasite by PCR is significantly more sensitive
than detection by microscopy.
cattle, DNA, PCR,
parasite, animal
biotechnology,
environmental
Tumor-specific Markers and
Indicators of Tumor Progression in
Melanoma Patients.
Fyfe et al.
Assessment of trypanosome
prevalence in FITCA high risk areas.
26 September 2003
using AmpFI STR Profiler Plus.
2002 Int J Legal Med 116:161-164 Buccal cell samples were collected on FTA and used as
template for the 9 STR Profiler Plus loci. These data were
used to determine the likelyhood ratios of paternity and
sibling relationships.
Buccal cells, human,
STR, paternity, sibling,
population study, human
id, forensics
Goldsborough
Room Temperature Archiving of
2000 Poster Abstract: Plant and
Demonstrates use for BAC and plasmid clones.
et al.
Plasmid Clones in an Automatable 96-
Animal Genome VIII San
Well Format
Diego, USA
glycerol stocks, overnight
cultures, resuspended
colonies, purified DNA, 2
Kb, 200 Kb, CloneSaver,
genomics
FTA cards were used to collect, store, and analyze blood
from nestling and adult birds (Great Grey Shrikes) for sex
determination.
birds, DNA banks, wildlife
ecology, PCR, population
studies, blood, nucleated
red blood cells, animal
biotechnology
Blood samples were collected on FTA. Single 1.2mm
punches were used in multiplex PCR amplifications in 5ul.
animal ID, population
study
Apple cider containing parasitic oocytes was spotted on
FTA and analyzed by PCR.
gastroenteritis, fecal
pellets, tissue, parasites,
infection, environmental,
food safety
Gaytmenn et al. Determination of the sensitivity and
specificity of sibship calculations
Gutierrez-
Using FTA cards to store avian blood 2002 Molecular Ecology Notes 2:
Corchero et al.
samples for genetic studies. Their
75-77
application in sex determination
Halbert et al
Conservation Genetic Analysis of the
Texas State Bison Herd
Hanes et al.
Inactivation of Cryptosporidium
parvum Oocytes in Fresh Apple
2004 J Mammalology 85(5):924931
2002 Appl Envir Microbiol. 68(8)
4168-4172
Cider by UV Irradiation
FTA Reference DatabaseJun05
9
Hansen and
Simple Archiving of Bacterial and
Blakesley
Plasmid DNAs for Future Use
Hartman and
Long Term Room Temperature
Lampel
Storage and Detection of Norovirus
on FTA® Filter Paper.
Harvey, ML
An alternative for the Extraction
and Storage of DNA from Insects in
1998 Focus 20(3) 72-74
2004 Poster: 104th General
Norwalk-like virus is a food borne pathogen. Norovirus is a
leading cause of non-bacterial gasteroenteritis. Diagnosis
Meeting of the American
is almost exlusively done by detection of virus in stool
Society of Microbiology, New samples. Norovirus positive stool was diluted 1:10 with
PBS and 200ul applied to FTA cards and dried overnight.
Orleans LA.
Cards were stored in plastic Whirl-Pak bags for 6 and 12
months at room temperature. One sample was stored for 2
years. A 6mm disk of FTA (approx 6 - 7ul stool) was
washed with 140ul sterile dist H2O. Viral RNA extracted
with QiaAmp Viral RNA Mini Kit (Qiagen). All samples
analyzed from FTA showed positive signal for Norovirus.
FTA makes sample collection, storage and transportation
easy and can be used to quickly detect Norovirus
outbreaks. FTA can facilitate epidemiological
investigations.
2005 J Forensic Sci. 50(3)
www.astm.org
Forensic Entomology.
Henderson et al. The long term outcome of limbal
allografts: the search for surviving
Bacterial genomic DNA was stored on an FTA card with
successful PCR amplifications. Also, plasmid DNA was
stored and transformed on FTA.
2001 Br J Ophthalmol 85:604 609
cells.
Hernandez et
Comparative Molecular Study of
al.
Populations of Common Crossbill
Conference of the European
(Loxia curvirostra ) on the Iberian
Ornithologists' Union
2003 Workshop WS02-P3 4th
Agrobacter tumefaciens,
plant bacteria, E. cole,
gram negative, gram
positive Streptomyces,
colonies, clones,
genomics
Virus, RT-PCR, food
borne pathogen,
detection, stool, feces,
vomit, rapid analysis
Samples of various life stages of flies were applied to FTA. insects, forensics,
A 3 fragments of 320, 650 and 1270 bp of a gene encoded entomology, mtDNA
in the mitochondrial DNA were amplified by PCR to show
the quality of the DNA. Authors found the 1.2mm punch to
be
optimal
for PCR
amplification;
2.0mm
punch
provided
FTA
disks were
pressed
against the
corneas
of eyes
impression cytology,
receiving limbal allografts to collect cell samples. The DNA human identification, ID,
from the cells were typed using 4 human STR markers to clinical, tissue grafts,
determine if they derived from the donor or the recepient. diagnostic
Blood samples from birds collected onto FTA cards and
prepared for PCR amplification of microsatellite markers.
microsatellites, avian,
blood, animal
biotechnology
Mitochondiral DNA analyzed from bird blood spotted to
FTA
birds, mitochondiral DNA
Peninsula and the Baleric Islands.
Hernandez et al. Identification of Lanius Species and
Subspecies using Tandem Repeats in
2004 Ibis 146:227-230.
the Mitochondrial DNA Control
Region.
FTA Reference DatabaseJun05
10
Hide et al.
A rapid and simple method of
2003 BMC Ecology 3:7
A specific protozoan, Bleparisma japonicum, detected in
environmental samples applied to FTA cards. Single cell
detection levels achieved. Larger number of cells 2.5 and
5 cell equivalents on FTA inhibited PCR. Canal, river and
pond water samples tested and B japonicum specifically
detected in the presence of other protozoans.
Protozoa, water samples,
environmental samples,
collection, storage
detection of Blepharisma japonicum
using PCR and immobilisation on FTA
paper.
Hickford et al.
Diversity of the Ovine DQA2 Gene
2004 J. Anim Sci. 82:1553-1563.
Blood from 2,000 sheep collected onto FTA cards. DQA2
gene was amplified by PCR cloned and sequenced.
genetic analysis,
agriculture, animal,
Higgins et al.
Sensitive and Rapid Identification of
1999 Ann NY Acad Sci 894: 130-
FTA was senstive for detecting Bacillis anthracis in buffer
and whole blood samples
anthrax, blood, biological
threat agents
FTA used to collect microorganism in liver tissue
real-time PCR, mouse
tissue, tick extract,
insects, gram negative,
infection, rapid detection,
bioterrorism, purified
DNA, blood, tail blood,
Dermacentor reticulatus,
Ixodes rincinus,
Haemaphysalis
concinna, PCR-EIA,
nested PCR,
environmental, parasite,
animal biotechnology
Biological Threat Agents
Higgins et al.
148
Detection of Francisella tularensis in 2000 Am J of Tropical Medicine
Infected Mammals and Vectors
and Hygiene 62:310-318
smears, then detection carried out by TaqMan PCR.
Using A Probe Based Polymerase
Chain Reaction
Ho et al.
Outbreak of Cyclosporaiasis
Associated with Imported
2002 Emerg. Infect. Dis. 8(8)
serial online
FTA used to collect food samples to detect parasite
infection.
Raspberries, Philadelphia,
Pennsylvania, 2000.
Howard et al.
food safety, DNA
extraction, ethyl acetate
extraction, FDA, CDC,
parasite oocytes,
produce, environmental
Standardizing Yields from Blood Card 2004 Poster: 15th Promega Human Punches of 3.2mm from BSD Duet from both FTA and 903 human id, forensics,
cards with blood samples were extracted with the Promega purified DNA, extracted
Punches Extracted with DNA IQ™
ID Symposium, Phoenix AZ
DNA IQ extraction system. Both yielded the same amount DNA,
and the Biomek™ Liquid Handler
of blood but yields from FTA was linear as more blood
punches were extracted. FTA yielded more DNA than 903
paper. Authors say storage conditions and DNA
degradation must be taken into effect when creating a
standard extraction method.
FTA Reference DatabaseJun05
11
Hsiao et al.
Application of FTA Sample Collection
1999 J. Clin Lab. Anal. 13: 188-193 Collection and processing tool for population samples
and DNA Purification System on the
for genotyping. FTA ability to preserve DNA against
Determination of CTG Trinucleotide
degradation is critical to this assay.
Repeat Size by PCR-Based Southern
gene mutation, myotonic
dystrophy (DM), deazadGTP, long term storage,
human, blood,
lymphoblast cells,
Blotting
Hunter et al.
The P28T Mutation in the GALK1
2002 Pediatr Res 51:602-606
Used FTA to collect blood from patients to do PCR on
genomic DNA.
gene mutation,
galactokinase gene 1,
human, blood, PCR,
screening,
microsatellites,
1998 Analytical Chemistry 70:
TaqMan PCR demonstrated for samples presented to
infectious disease,
genetic disorders, single
nucleotide polymorphism,
SNP, orthopoxvirus,
cowpox, monkeypox,
camelpox, vaccinia
subspecies buffalopox,
human, blood,
Gene Accounts for Galactokinase
Deficiency in Roma (Gypsy) Patients
Across Europe.
Ibrahim et al.
Real Time Microchip PCR for
Detecting Single-Base Differences in
2013-2017
and stored on FTA cards
Viral and Human DNA
Igoe et al.
A Novel Automatable System for the 2001 Poster: 11th Genome
Demonstrates the capabilities of the CloneSaver card
Room Temperature Archiving and
Sequencing and Analysis
for storage of plasmid and BAC clones including phage
Recovery of DNA Clones
Conference, San Diego, USA
inactivation, glycerol stock application, no cross-
phage inactivation, cross
contamination, 3 yr
plasmid archiving,
contamination, and archiving capabilities.
Igoe et al.
A High-Throughput System for Room 2002 Poster: 12th Genome
Temperature Archiving and
Sequencing and Analysis
Processing of Plasmid and BAC
Conference, Boston MA
Clones.
Igoe et al.
Purification and Analysis of DNA
2003 Poster: IX Plant and Animal
Demonstrates a prototype of a "hard" CloneSaver,
HardCard, Biomek 2000,
automated punch
automated sample application of plasmid DNA using the
washing, rolling circle
Biomek 2000 and the use of FTA as a template for
amplification,
Templiphi™ template amplification kit.
FTA and CloneSaver punches were washed using the
from Solid-Phase Samples Using an
Genome Conference, San
Biomek 2000 liquid handler in an automated fashion.
Automated Platform.
Diego, USA
Studies proved no cross contamination between wells.
Igoe and
Amplifying Stored Plasmids for
Robbins
Sequencing
2004 Genetic Engineering News
24(2): 34
Describes using Amersham's TempliPhi kit to amplify
plasmid DNA stored on CloneSaver. Plasmid DNA was
eluted from CloneSaver and amplified with TempliPhi.
automated punch
washing, plant DNA,
blood, plasmid,
transformation
Plasmid, clones,
genomics, rolling circle
amplification,
The amplified plasmid was cut with restriction enzymes
or was sequenced using Applied Biosystems BigDye v 3.0
kit.
FTA Reference DatabaseJun05
12
Igoe et al.
Comparison of DNA Stability on
Treated and Untreated Papers.
2004 Poster: American Academy of Purified DNA applied to FTA was protected from UV
Forensic Science Dallas TX
damage compared to DNA on untreated filter paper.
untreated filters, DNA
stability
DNA was more stable on FTA over time and storage
conditions compared to untreated filter paper. Blood
applied to glass fiber media treated with FTA was
stable after 5 mos where as DNA on untreated media
was completely degraded and not amplifiable by PCR.
Ivanov et al.
The Experience of using the FTA®
Cards for Blood Samples Storage at
2002 Sudebno-Meditinskaya
Ekspertiza 45:20 - 27.
PCR amplification of mitochondrial DNA; HVS-1 and -2
(hypervariable segments) of the D-loop. Analysis of
Conducting the Molecular-and-
PCR fragments from Profiler Plus system on ABI Prism
Genetic Identification of Non-
377. Describes benefit of room temperature storage
identified Killed Persons - Victims of
over traditional storage methods. Blood on FTA stored
Military Actions in the Territory of
from several weeks to 3.5 years. Describes benefit of
the Chechen Republic
FTA over phenol-detergent or Chelex extractions of
Jankowski et al. The Comparison and Validation of
2000 Poster 11th International
FTA, CHELEX, and Qiagen
Symposium on Human ID,
Extraction Methods on Blood
Biloxi, LA
mtDNA, missing persons,
military, 1100 blood
samples, human,
DNA.
Analysis of several methods of Processing FTA for STR extract DNA from FTA,
profiling.
Spotted FTA Cards for the Analysis
of the 13 Core STR Loci
Johnson
A PCR-Based Method for Detection
of Shigella in Produce Washes.
Jones et al.
The Use of FTA® for the
2003 Presentation AOAC
Compare FTA to Qiagen Dnease Plant Mini Kit in
International Midwest
preparing Shigella for PCR analysis from plant washes.
Section Meeting and
Also compare BAX real-time PCR detection system to
Exposition
PCR and gel electrophoresis. Both FTA and Qiagen
2003 Poster: San Diego
prepared a good template.
Spore-forming bacteria and gram positive bacteria
Identification of Gram Positive and
Conference: Cool Tools,
applied to FTA and analyzed by PCR for 16S RNA gene,
Spore-Forming Bacteria.
Baltimore MD Oct 30 - Nov
esp and gdh genes. Bacterial genomic DNA could be
1, 2003
detected at a dilution of 10-7 showing that FTA
Real-time PCR, BAX
automation detection
system, bacteria,
produce, pathogens,
gram positive bacteria,
spores,
provides a sensitive level of detection.
Jou et al.
Delineation of CTG Repeats and
2001 Proc. Natl. Sci. Counc.
Clinical Features in a Taiwanese
ROC(B) 25 (1) 40 -44.
Mytotonic Dystrophy Family.
FTA used to collect patient buccal cells and prepare
clinical, gene mutation,
DNA for PCR amplification to detect genetic alterations buccal, human,
in the myotonin protein kinase gene in myotonic
dystrophy.
FTA Reference DatabaseJun05
13
Karle et al.
Novel filter-based technology for
simple collection and preparation of
PCR-ready plant DNA:applications for
2001 Poster: 11th Genome
Sequencing and Analysis
Conference
Demonstrates processing plant samples for DNA
analysis using FTA and GenSpin Kit for Plants
transgenic plant detection
alfalfa, Arabidopsis,
Brassica, corn, cotton,
cucumber, potato, diploid
potato, rice, ryegrass,
soybean, spinach,
tomato, wheat, SSR
amplification, transgenic,
Bt-Cry3A, Rca gene, rbcL
gene, SATT309,
nematode resistance,
GMO
Karle et al.
Application of FTA-based Technology 2002 Poster: Xth Plant and Animal Demonstrates the utility of FTA for collection, storage, alfalfa, Arabidopsis,
Brassica, corn, cotton,
for Simple Collection, Transport,
Genome Conference, San
simple DNA purification, and DNA identification of
cucumber, potato, diploid
Purification and Storage of PCRDiego, USA
plants
potato, rice, ryegrass,
soybean, spinach,
ready plant DNA.
tomato, wheat, SSR
amplification, transgenic,
Bt-Cry3A, Rca gene, rbcL
gene, SATT309,
nematode resistance,
GMO
Kerlin et al.
Survival Advantage Associated with
2003 Blood 102(9)3085-3092
Whole blood collected from 1605 patients sent to Lilly
Heterozygous Factor V Leiden
Research Labs to analyze mutations in Factor V gene by
Mutation in Patients with Severe
PCR
septic shock, mutation
analysis, clinical trial
Sepsis and in Mouse Endotoxemia
Kline et al.
Polymerase Chain Reaction
Amplification of DNA from Aged
2002 Analytical Chemistry 74:
1863-1869
NIST evaluated four different card matrixes for STR
multiplex performance from dried blood stored on the
Blood Stains: Quantitative Evaluation
cards for 19 months. Publication also demonstrates the
of the "Suitability for Purpose" of
ability to elute DNA off of FTA Cards using a Chelex
Four Filter Papers as Archival Media
extraction procedure.
FTA Reference DatabaseJun05
Blood stain paper, S&S
903, IsoCode, FTA,
chelex, blood, human,
14
Kozlowski et al. SNP and Transgene Analysis Directly 2003 Poster: Plant & Animal
from Sample Collection Paper using
the Invader® DNA Assay.
Krenke et al.
Validation of a 10-Locus Flourescent
Multiplex System
Kuboki et al.
Loop-Mediated Isothermal
Amplification for Detection of
African Trypanosomes
LaFountian et al. TWGDAM Validation of the
AmpFlSTR Profiler Plus and the
FTA used in Third Wave Invader Assay to detect SNPs in
pigs/sheep and also transgenes in soybean/corn. Each
Genomes XI Conference, San
reaction was a biplex, measuring 2 targets per reaction.
Diego, CA
S&S 903 and untreated blotting paper were also tested.
Samples were blood or leaf material and 2mm punches
were assayed in 96 well plates. Leaf punches were
washed in 85% ethanol; blood punches were washed in
water; then punches were incubated in water for 10 min at
95oC. The Invader reagents were added and incubated
65oC for 2 - 4hrs. Results from FTA cards were similar to
results from genomic DNA purified via Gentra DNA kit; 903
and untreated blotting paper did not work well and require
more optimization. Blood samples and soybean samples
on FTA worked great, corn needs more optimization.
2002 J Forensic Sci 47(4):773785
2003 J. Clin Microbiol 41(12)5517- FTA used to collect blood from mice and cattle infected
with protozoan parasites. Washed FTA punches were
5524.
used in both PCR and a non-PCR based amplification
reaction, an isothermal (all at one temperature) reaction
2001 J Forensic Sci 46(5):11911198.
AmpFlSTR Cofiler STR Multiplex
Systems Using Capillary
Electrophoresis
Lam PF and
Rapid PCR Screening of Transgenic
Shaari A
Pineapple using FTA Cards
An STR kit was validated in several forensic labs under a
variety of conditions. Dried blood on FTA either amplified
directly or was extracted by Promega DNA IQ system.
FTA punches performed better if the standard number of
PCR cycles was 10/20 to 10/22.
2003 Poster: 14th Malaysian
Society of Plant Physiology
Conference, Awana Genting,
SNP, porcine, ovine,
blood, scrapie, stress
syndrome, transgenic
plants, GMO
forensic, validation
experiments, human ID
parasites, diagnostic,
PCR,
The two STR kits were validated in a forensic lab with a
forensic, validation
variety of sample types from purified DNA to blood on FTA experiments, human ID
to complex sample types (blood on blue denim). Many
parameters were tested both PCR and capillary
electrophoresis. DNA from blood on FTA performed as
well as genomic DNA purified by proteinase K and organic
extraction.
Leaves of transgenic and non-transgenic pineapple were
transgenic plants, plant,
crushed onto FTA cards and examined for the presence of agriculture
the transgene by PCR
Malaysia
FTA Reference DatabaseJun05
15
Lampel et al.
Improved Template Preparation for
PCR- Based Assays for Detection of
Food-Borne Bacterial Pathogens
Lampel KA &
Rapid detection of pathogenic
Orlandi PA
microbes by PCR using a non-
2000 Applied and Environmental
FTA based technique for the detection of food
2002 Poster IUMS International
The inherent properties of FTA filters allows PCR
Shigella flexneri,
Salmonella enterica,
Microbiology 66: 4539-4542 microbes in wash solutions for produce & beef. Shigella
Listeria monocytogenes,
and Salmonella are gram-negative easy to lyse and
gram-negative, grampositive, produce, wash
fewer are detectable on FTA; Listeria are gramprocedure, ground beef,
positive harder to lyse need more to detect on FTA.
boiling, food safety,
screening,
Conference, Paris
template preparation for microbial organisms
extraction template protocol
Lampel,KA, Dyer Detection of Bacillus Spores Using
D, Kornegay L
PCR and FTA Filters.
2004 J Food Protection 67(5)1036- Difficult to lyse spores from Bacillus subspecies
cereus, thruingiensis, subtilis and niger were isolated
1038.
and Orlandi PA
bacteria, environmental,
food safety,
and applied to FTA cards. A fragment of the 16S rRNA
gene was amplified by PCR. Sensitivity of approx 50
spores were detected and this was increased to 5
Lampel et al.
Real-Time PCR Detection of Shigella
From Foods Using FTA Filters.
2004 Poster: 104th General
spores by nested PCR.
Shigella flexneri were added ("spiked") to apple cider,
Meeting American Society of strawberries or cilantro. PBS (10ml) was used to wash
Microbiology, New Orleans
berries and cilantro. An 10ul aliquot of food wash or
bacteria, pathogens, food
borne illness, food safety,
detection, nested PCR
spiked cider was applied to FTA. After sample
application the filters were washed with ethanol then
dried at 56oC. The filters were then washed twice with
FTA reagent then twice with TE-1 and dried at 56oC.
Disks of 6mm were cut out and added to 50ul TE and
heated at 95oC for 10 min to elute DNA for real-time
PCR. Nested real-time PCR done on a LightCycler with
SYBR Green incorporation and by hybridization probe
displacement. Both real-time PCR methods can be
successfully used with FTA. Using FTA a sensitivity
level of less than 10 organisms cam be achieved from
Lappas et al.
The utility of the FTA GeneCard
system for analysis of buccal swab
1999 Poster 10th International
Symposium on Human ID
pure culture or seeded foods.
FTA solves the problem of DNA extraction & shipping
issues.
150bp, >12Kb, stable
DNA, intact DNA
transfers
Ledray & Netzel Forensic Nursing: DNA Evidence
Collection
1997 Journal of Emergency
Nursing 23:156-158
Describes use of FTA in blood collection and storage
blood, victim, databasing
from victim and/or perpetrator in rape cases
FTA Reference DatabaseJun05
16
Li et al.
Persistence of Human
Immunodeficiency Virus Type 1
2004 J. Clin. Microbiol. 42(8)3847- HIV-1 DNA is stable on FTA cards for over 4 years
3849.
HIV, DNA stability
from time of collection. Previous data only showed
Subtype B DNA in Dried-Blood
stability on untreated paper as long as 15 months.
Samples on FTA Filter Paper
Lim SES and
Evaluation and Modification of the
Tan WF
IsoCode Card DNA Isolation Method
2002 Poster: 16th Meeting of the
Blood was applied to FTA and to IsoCode. IsoCode
IsoCode, blood
International Assocication of yields amplifiable DNA and is comparable to the results
Forensic Science, Montpellier achieved with their normal methods using FTA
France, September
Lin et al.
Detection of Plant Genes Using a
2000 BioTechniques 28: 346-350
Rapid, Nonorganic DNA Purification
FTA used to process PCR ready template from a wide
variety of plant samples
Method
Littlejohn et al. Determination of b2-Adrenergic
Receptor (ADRB2) Haplotypes by a
2002 Human Mutation Mutation in
Brief #562, Online.
Multiplexed Polymerase Chain
Reaction Assay.
MacLean L, Chisi Severity of Human African
JE, Odiit M,
Trypanosomiasis in East Africa is
Gibson WC,
Associated with Geographic Location,
2004 Infection and Immunity
72(12):7040-7044
Peripheral blood was either applied to FTA cards or DNA
ARMS assay, SNP,
extracted by the following methods, guanidine
multiplex, clinical study
isothiocyanate, sodium hydroxide boiling lysis or salting
out. FTA punches or extracted DNA was then analyzed in
a multiplex PCR assay to detect SNPs in the ADRB2 gene.
Results showed that FTA was not as efficient in this study
as DNA extracted by the other methods.
During surveillance for typanosomaisis, 200ul of a buffy protozoan, protozoa,
population study, disease
coat suspension (primarily white blood cells) were
detection
applied to FTA cards which where then dried at room
Ferris V, Picozzi Parasite Genotyp, and Host
temperature to detect trypanosome DNA by PCR
K and Sternberg Inflammatory Cytoking Response
amplification of 2mm punches
JM
Arabidopsis, marijuana,
Cannabis, cassava, coca,
corn, orchid, papaya,
petunia, opium poppy,
soybean, sugarbeet,
sugarcane, tobacco,
tomato, transgenic, uidA
gene, CTAB, plant
DNAzol, 18S rRNA gene,
rbcL gene, soybean cyst
nematode race 3
resistance gene,
monocot, dicot, marker
assisted breeding,
selection,
Profile
FTA Reference DatabaseJun05
17
Martinez-
Biological Stains Collected from
Gonzalez et al.
Crime Scenes using FTA™ Paper.
Martins et al.
Haplotype Study of Microsatellites
crime scenes, sample
2005 Poster: 57th Annual Meeting FTA was used to blot blood and semen stains from nonabsorbent (glass, tile, wood) and from absorbent (carpet)
recovery
of the American Association
surfaces. First wet the stain with distilled water then blot
of Forensic Scientists, New with FTA cards. This method is quick, easy and preserves
the original stain since only some of the cells are
Orleans LA.
transferred. This method is very applicable to analysis of
biological stains at crime scenes.
2002 Leukemia 16:1353-1357.
Flanking the t(15;17) Breakpoint in
Acute Promyelocytic Leukemia
FTA used to collect blood from unrelated, healthy
human, blood, acute
individuals and blood from PML patients and their families. promyelocytic leukemia,
DNA was extracted from FTA using chelex.
PML, microsatellites,
PCR, eluted DNA, chelex
Patients from North Portugal
Matsumoto et
Tiburonia granrojo n. sp., A
al.
Mesopelagic Scyphomedusa from the
Pacific Ocean Representing the Type
2003 Marine Biology
FTA used in the identification of a new jellyfish species.
Tissue was pressed on to FTA which then was PCR
10.1007/s002227-003-1047amplified.
2 (online journal)
jellyfish, tissue, 28S
rRNA gene, PCR,
sequencing, new species,
genotyping
of a New Subfamily (class
Scyphozoa: order Semaeostomeae:
family Ulmaridae: subfamily
Tiburoniinae subfam.nov.)
Meadus and
Simplifying Genetic Testing from
MacInnis
Porcine Hair and Blood Samples
Mills et al.
Effect of Immunosuppression on
Outcome Measures in a Model of Rat
2000 Presentation: Banff Pork
Seminar
2002 Invest Opthalmol Vis Sci.
43(3)647-655
FTA used to collect blood from ear vein prick of porcine
livestock.
non-invasive, genetic
testing, pigs,
FTA was used to collect cells from coreal transplants in
rats to identify the presence of donor cells by PCR.
eye tissue,
transplantation, tissue
grafts, limbal tissue
Shows the use of BioMek 2000 for washing punches from
blood, buccal and plant samples
Automation of FTA
Limbal Transplantation.
Moran et al.
High Throughput Purification of
Samples Archived on FTA® Cards
2003 Poster:14th International
Symposium on Human ID,
Phoenix AZ
Moran et al.
The use of FTA® for the
Identification of Bacteria from
Culture Collections and Clinical
Isolates.
Moran et al.
Comparison of DNA Stability on
Treated and Untreated Papers.
2004 Poster: 104th General
Bacterial ID,
environmental, clinical,
microbiology, gram
positive
2004 Poster: 56th Annual Meeting Shows studies of degradation of DNA on untreated filter
paper compared to preserved DNA on FTA. Accelerated
of the American Association
aging by UV radiation of the DNA showed that FTA is
of Forensic Scientists, Dallas superior to untreated filter papers for preserving the
integrity of DNA.
TX
UV radiation, aging, DNA
stability
Demonstrates use of FTA for characterizing bacterial
genomic DNA samples. PCR amplification of species
Meeting of the American
specific genes and RAPD analysis. Some gram positive
Society of Microbiology, New species of Streptococcus and Peptostreptococcus were
used in this study.
Orleans LA.
FTA Reference DatabaseJun05
18
Moran B and
Analysis of Cattle Samples on FTA®
2005 Poster: XIII Annual Plant
Layton R
Cards using Stockmarks® Genotyping
and Animal Genome
Kit
Conference, San Diego CA
Morton et al.
A Simple Heat Elution of Human
Genomic DNA from FTA® Treated
2000 Poster: 11th International
Symposium on Human ID
Paper Using Water
Moscoso et al.
Moscoso et al.
Moscoso et al.
PCR Detection of Mycoplasma Stored 2003 Poster: World Vet. Poultry
and Transported on FTA Gene Guard
Assn & 140th AVMA Annual
® Filter Paper
Convention, Denver CO
Molecular Identification of Avian
RNA Viruses Stored on FTA™ Filter
Poultry Disease Conference,
Paper.
Sacramento CA.
Indentification of Infectious
Bronchitis Virus by RT-PCR from
FTA Filter Paper
Moscoso H,
Application of the FTA Technology
Thayer SG, and for the Collection, Shipment and
Hofacre CL
2004 Poster: 53rd Western
Processing of Avian DNA and RNA
Viruses
Blood and buccal samples were collected from Holstein
cattle, animal ID,
cows and applied to FTA cards. Samples were analyzed
genotyping, SNP analysis
by the ABI Stockmarks genotyping kit which employs
microsatellite markers and also by SNP analysis. The data
were very similar for both analytical methods although the
SNP analysis could analyze more difficult samples. FTA
proves to be useful for collecting cattle samples for both
PCR and SNP analysis.
FTA benefits of destroying potentially harmful pathogens
eluted DNA, GenSpin,
and long term room temperature storage. Heated water
quantitate DNA, DNA in
eluted enough DNA to quantify and be used in downstream solution,
forensic analysis.
Used FTA to detect two subspecies of mycoplasma. Fluid
containing live infectious bronchitis virus and Newcastle
Disease virus were inactivated after 1 hr on FTA. Also
both species of mycoplasma were inactivated after being
spotted onto FTA. Both purified cultures and field collected
samples from chickens were spotted onto FTA and
analyzed by PCR for the 16S rRNA gene.
viral inactivation,
pathogen inactivation,
chicken, diagnostic,
animal biotechnology
RNA viruses Infectious Bronchitis Virus, Infectious Bursal
Disease virus and Avian Leukosis Virus (subgroup J) from
chicken samples were applied to FTA. FTA inactivated the
viruses. RNA was extracted from the cards and was
analyzed by RT-PCR. RT-PCR was specific for the target
virus. RT-PCR from bursa on FTA can detect as little as 5
ng of RNA. RNA is stable on FTA cards.
chicken, bursa, tracheal
swab, allantoid fluid,
tumors, liver, RFLP,
strain differentiation,
pathogen inactivation
2004 Poster: International Poultry FTA was used to collect and store 5ul aliquotes of allantoic
fluid containing live virus. Cards were stored at room
Scientific Forum, Atlanta GA
temp, 4oC and 41oC before analysis. Two mm disks were
washed in TE-1, vortexed and incubated at room temp 15
min. RNA was extracted using High Pure Viral RNA Kit
(Roche). RNA served as a template for RT-PCR and
RFLP. Live virus was inactivated after application to FTA.
FTA was sensitive for RNA detection. RNA on FTA stored
at 41oC for 15 days still showed good RT-PCR
demonstrating the protectective effect of FTA.
2004 Poster: 141st AVMA Annual
Convention Philadelphia, July
viral inactivation,
pathogen inactivation,
chicken, diagnostic,
animal biotechnology,
RNA stability
Adenovirus and ILTV are DNA viruses, New Castle
disease diagnostics, DNA
Disease virus is ssRNA virus. These were detected using virus, RNA virus,
samples of liver, tracheal swab or tissue culture samples
inactivation
applied to FTA. RNA was eluted from the disks in Tris
EDTA buffer, extracted then amplified by RT-PCR for NDV.
As little as 5 ng of RNA virus was detected on FTA.
FTA Reference DatabaseJun05
19
Moscoso et al.
Inactivation, Storage and PCR
2004 Avian Diseases 48:841-850.
FTA was used to detect two species of Mycoplasma in
pure cultures and in field specimens. 193 field samples
were spotted to FTA, analyzed by PCR and the results
compared to classical serology methods of detection.
Nearly 100% agreement occurred between FTA and
classical methods. FTA completely inactivated the
mycoplasma and serves as a safe and cost effective
method to transport samples to the diagnostic lab.
disease diagnostics,
avian disease, tissue
samples, tracheal swabs,
2005 Avian Diseases 4 9:24-29
Infectious Bronchitis Virus (IBV) is an RNA virus. RT-PCR
assays were devloped to detect IBV and variants. Virus
propagated in allantoic fluid or tracheal swabs from
chickens were applied to FTA cards. Tests showed that
virus applied to FTA cards was inactivated the virus after
application to the cards making international shipment of
test samples to the diagnostic lab easy. RNA was
extracted from the FTA disks using the High Pure Viral
RNA Kit (Roche) or using Trizol. RNA was stable on FTA
with only a slight decrease detected after 15 days at 41oC.
virus, RT-PCR, RNA
virus, viral inactivation,
veterinary diagnostics,
RNA stability,
Detection of Mycoplasma on FTA
Filter Paper
Moscoso et al.
Molecular Detection and Serotyping
of Infectious Bronchitis Virus from
FTA® Filter Paper
Moscoso et al.
Molecular Characterization of Fowl
Adenoviruses from FTA® - Liver
Impressions
2005 Poster: 54th Western Poultry Inclusion body hepatitis (IBH) is caused by adenoviruse DNA virus, liver samples,
tissue, viral inactivation,
Disease Conference.
group I and can now be diagnosed by molecular
sequencing
Vancouver Canada April 25 - techniques. Sections of infected liver was applied to
27
FTA by squashing the tissue onto the card. Adenovirus
was shown to be inactivated. Several serotypes of virus
were detected by PCR after 8 months of storage on
FTA. Sequencing of amplicons showed 100% homology
to the challenging virus so no change in sequence
occured after storage on FTA. This is the first time
adenovirus was detected from liver impressions on FTA
cards.
Moscoso et al.
Detection of Infectious
laryngotracheitis Virus by PCR on
2005 Poster: 54th Western Poultry Infectious Laaryngotracheitis viral (ILTV) disease had DNA virus, conjunctivitis,
viral inactivation
Disease Conference.
been detected by histopahtology or direct antibody
Specimens Stored on FTA® Filter
Vancouver Canada April 25 -
detetion taking over a day to diagnose. Molecular
Paper
27
detection of the disease on FTA has greatly speeded up
the diagnosis process. Sample types on FTA included
eyelid impressions, trachea impressions, tracheal
scrapings and tracheal swabs. ILVT was inactivated on
FTA. ILTV was detected from FTA by PCR. Eyelid
impressions served to be the best souce of sample for
FTA cards and 100% successful diagnostics.
FTA Reference DatabaseJun05
20
Nakayama et al. Clinical Significance of Circulating
2000 Ann NY Acad Sci 906; 87-98 FTA used to collect blood from patients with melanoma
Loss of heterozygosity,
human, cancer study,
clinical, diagnostics
2000 BioTechniques 29: 1328-1333 FTA used for storing & analyzing RT-PCR mRNA from
HeLa cells, BHK-21 cells,
cell culture, blood,
buccal, plant, leaf, potato,
poly(A)+ mRNA, total
RNA, Trizol, Northern
blot,
DNA Microsatellite Markers in
Plasma of Melanoma Patients
Natarajan et al. Paper-Based Archiving of Mammalian
and Plant Samples for RNA Analysis
plant & tissue culture cells- several months ambient
storage (longer -70C) 2mm punch for RT-PCR,
2005 Virology J 2:45
Both DNA and RNA viruses were detected in several plant
species after application to FTA. The FTA method is
comparable to tradtional methods of recovering RNA and
DNA viruses and FTA simplifies the sampling and analysis
of diseases plants in both the lab and the field. Amplified
sections of DNA were cloned into sequencing vectors and
the sequences compared at the 99.8% level to DNA
purified by traditional methods
2004 Int J Systm Evol Microbiol.
epierythrocytic agent of haemolytic
Blood samples from sheep were deposited on FTA. The bacteria, pathogens,
animal biotechnology,
16S rRNA gene of a red cell parasite was amplified by
mycoplasma
PCR. The results showed that the parasite though to
anaemia in sheep and goats.
be a member of Rickettsia is acutally a member of the
Ndunguru et al. Application of FTA Technology for
Sampling, Recovery and Molecular
Characterization of Viral Pathogens
and Virus-derived Transgenes from
Plant Tissue.
Neimark et al.
Mycoplasma ovis comb. Nov.
(formerly Eperythrozoon ovis), an
54:365-371.
geminiviruses, maize,
cassava, tomato,
tobacco, Tobacco mosaic
virus, Potato Virus Y and
Tobacco etch virus,
ssDNA virus, East
African cassava mosaic
Cameroon virus, African
cassava mosaic viurs,
Tomato yellow leaf curl
virus
mycoplasma family of pathogens.
Neimark et al.
Phylogenetic analysis and description
of Eperythrozoon coccoides ,
2005 Intl J Systematic Evol
Microbiol. 55:1385-1391
proposal to transfer to the genus
Mycoplasma as Mycoplasma
coccoides comb. nov. and Request for
Blood from mice infected with E. coccoides was applied to phylogenetic tree
construction, blood
FTA cards. Three mm punches were washed with FTA
parasites,
purification reagent and TE (not TE-1) and used as
template for 16S rRNA gene PCR. Amplicons were
sequenced and sequences compared to other species of
Mycoplasma .
Opinion
Nelson et al.
Detection of a Primer-Binding Site
Polymorphism for the STR Locus
D16S539 Using the PowerPlex 1.1
System and Validation of a
2002 Journal of Forensic Science
47 (2): 345-349
DNA database sample discrepancies were caused by the
amplification conditions (AmpliTaq vs. AmpliTaq Gold)
when using Promega's PowerPlex 1.1 typing kit and not by
the different DNA extraction methods (FTA vs. organic
extraction).
allele dropout, hotstart
PCR, DNA typing, point
mutation, polymorphism,
imbalanced alleles,
Degenerate Primer to Correct for
Polymorphism
FTA Reference DatabaseJun05
21
Nields et al.
Evaluation of the FTA DNA
Collection and Storage System on
1998 Poster American Academy of FTA for blood processing of human autopsy cases.
Forensic Sciences
Samples taken up to 12 hr after death.
medical examiner,
autopsy,
Autopsy Blood from Medical
Examiner Cases
Norton and
Whole Genome Amplification on
Shriver
FTA® Cards.
2003 Poster: IX Plant and Animal
DNA from blood spots collected on FTA was used as a
Genome Conference, San
template in Whole Genome Amplification (WGA).
Diego, USA
Punches were washed using a Hydra liquid handler and
rolling circle amplification,
Phi DNA polymerase,
multiple strand
displacement, MDA,
amplified using a Templiphi kit (Amersham). After
WGA, 15 different loci were amplified for analysis with
O'Shea et al.
Amplified Fragment Length
average success rates of 90-96%.
2004 J. Clin. Microbiol. 42(8) 3600- M. avium subsp. paratuberculosis causes Johne's
Variability among Mycobacterium
gram positive bacteria,
pathogen, cattle, clinical
disease a debilitating disease in cattle. Twenty isolates
diagnostics
of this pathogen were spotted to FTA cards. FTA
avium subsp. paratuberculosis
punches were washed according to the standard
Isolates.
protocol then 7.1µl of ddH2O were added and the punch
Polymorphism Reveals Genomic
3606.
heated to 94oC for 5 min, 12.9µl of master mix were
Orlandi &
Extraction Free, Filter-based
Lampel
Template Preparation for Rapid and
2000 Journal Clinical Microbiology
38: 2271-2277
added for PCR amplification.
FTA used to isolate protozoan oocyte DNA from soft fruit
and stool - sensitive detection and time-saving procedure.
Sensitive PCR Detection of
Pathogenic Parasitic Protozoa
Orlandi et al.
Targeting Single Nucleotide
Polymorphisms in the 18S rRNA Gene
2003 Appl. Envron. Microbiol.
69:4806-4813.
to Differentiate Cyclospora species
and Eimeria species by Multiplex
PCR.
Orlandi and
FTA Filter Applications: PCR Format
Lampel
for Alleviating Technical Barriers in
the Detection of Food-borne
Pathogens in Complex Matrices.
2003 BioProcessing J 2(6)45 -54.
Parasite oocytes were applied to FTA to prepare DNA
template for both nested PCR or PCR followed by RFLP to
differentiate species of Cyclospora and Eimeria. Raw fecal
material was spotted to FTA and subjected to SNP
Multiplex PCR. This method quickly identifies the species
designation of clinical isolates of parasites.
parasites, Cyclospora,
Cryptosporidium,
microsporidia,
Encephalitozoon
intestinalis, spores,
multiplex PCR, food,
safety, screening, fecal
specimens, feces,
Parasite, fecal, feces,
RFLP, SNP, multiplex,
species specific,
environmental
Technology review of the current methods of detecting food- Parasites, microbes,
borne pathogens vs using FTA as a faster and simpler
bacteria, pathogens, food
method for preparing DNA for analysis. Current methods safety, environmental
of growth enrichment or selection are not necessary using
the FTA method thus shortening the prep time
dramatically.
FTA Reference DatabaseJun05
22
Patel AA
PCR Detection of Streptococcus
Mutans and Streptococcus Sobrinus
2002 MA Thesis Virginia
Commonwealth University
in Dental Plaque Samples from Low,
Moderate and High Caries Risk
Children
Prager et al.
Chlamydia pneumoniae in Carotid
Whole blood collected from patients with atherosclerosis
human, bacteria,
and DNA prepared by Miller salting out method and also
pathogen, diagnostic,
spotted to FTA. No difference was seen between DNA
microbes,
isolated by either method when analyzed with PCR for the
presence of the infectious bacterial pathogen Chlamydia
pneumoniae . The 16S rRNA gene fragment was amplified
for identification of the pathogen.
2002 African Journal of
PCR on buffy coat preparations using Whatman FTA®
matrices …were the gold standard in the field of sleeping
sickness.
Comparison of its Presence in
Atherosclerotic Plaque, Healthy
Vessels, and Circulating Leukocytes
from the Same Individuals.
The diagnosis of trypanosome
infections: applications of novel
Biotechnology
1 :39-45
technology for reducing disease risk.
Picozzi et al.
Diagnosis of Trypanosoma brucei
infections in cattle: applications of
novel technology for estimating
disease risk.
Pierce et al.
2003 ICPTV Newsletter 8 pg 24 - Comparison of DNAzol, whole blood on FTA and buffy coat
on FTA to prepare DNA from cattle for screening of the
25 September 2003
trypanosome parasite that causes sleeping sickness in
humans. The parasites were detected by PCR analysis of
the DNA. Advantages of the FTA are easy field collection
and the ability to re-analyze samples due to the long
storage capacity of FTA cards
High Throughput DNA Purification of 2002 Poster: 12th Genome
Samples Archived on FTA® Cards.
Sequencing and Analysis
Application of DNA Fingerprinting in
Medicolegal Practice
2002 J. Indian Med. Assoc.
100(12) 688-694
parasites, cattle,
trypanosome,
Trypanosoma brucei
rhodesiense,
Trypanosoma brucei
brucei, T. vivax, T.
congolense, livestock,
screening, animal health,
field testing,
parasite, buffy coat,
whole blood, cattle,
disease, diagnosis, PCR,
field collection, storage
Demonstrates the use of Biomek 2000 in washing FTA
punches prior to PCR analysis.
automation, liquid
handling, blood, plant,
buccal, STR,
Use of FTA for blood sample collection, archive and
microsatellites, biological
samples
Conference, Boston MA
Raina & Dogra
bacterial id, clinical
microbiology, disease
diagnostics, human,
dental disease
2002 Stroke 33:2756-2761.
Artery Atherosclerosis A
Picozzi et al.
Plaque samples were taken from volunteers with a sterile
toothpick and placed into 25ul bufffer and frozen. After
thawing 10ul were spotted to FTA cards. A 2mm punch
was taken and washed as normal. A 50ul PCR master mix
was added to the punches and amplified for the specific
bacteria under examination.
DNA preparation for PCR based DNA fingerprinting
(STR analysis). Mentions features and benefits of FTA,
i.e., easy, can archive samples at room temp, no
quantitation is necessary from the punch and punch can
be reused in another PCR.
FTA Reference DatabaseJun05
23
Rajendram et al. Microbial Applications of FTA
Technology
2002 Poster IUMS International
Conference, Paris
Bacterial strains and clinical isolates stored on FTA.
Ribotyping of bacteria with 16s rRNA gene done
successfully on gram positive and gram negative
bacteria. Not all spores were lysed and inactivated.
Rajendram
DNA Storage and Recovery for DNA
Fingerprinting and Gene Detection
2003 Presentation PHLS/Whatman 400 strains of bacteria tested on FTA; gram-negative,
symposium, London
using FTA Paper Technology
Rejt et al.
Corynebacterium,
Nocardia,
Mycobacterium, Bacillus,
Neisseria,
Staphylococcus,
Streptococcus,
Enterococcus, Bordetella,
Legionella,
Pseudomonas,
Pasterurella, Aeromonas,
Haemophilus, Shigella,
Citrobacter, Proteus,
Moraxella, vibrio,
Actinobacillus,
Peptostreptococcus
corynebacterium,
Nocardia,
gram-positive and spore formers. 16S rRNA gene
Mycobacterium, Bacillus,
amplified by PCR as well as single copy genes showing
Neisseria,
Staphylococcus,
sensitivity of FTA DNA prep. Archive bacterial DNA
Streptococcus,
on FTA for up to 1 yr. AFLP and RAPD analysis done.
Enterococcus, Bordetella,
FTA inactivates bacteria at low to moderate titer. At Legionella,
Pseudomonas,
high titer some gram-positive are viable. Additional
lysis with guanidine thiocyanate inactivates all bacteria. Pasterurella, Aeromonas,
Haemophilus, Shigella,
Citrobacter, Proteus,
Moraxella, vibrio,
Actinobacillus,
Peptostreptococcus,
Clostridium, ribotyping
Genetic Variability of Urban Kestrals 2004 Zool. Poloniae 49: 199 - 209
Kestrals are birds of prey like hawks. Blood samples
in Warsaw - Preliminary Data
from the birds were collected on FTA cards. Punches,
2mm, were included in 25µl reactions to amplify
Birds, population study,
ecology, population
diversity
microsatellite markers.
FTA Reference DatabaseJun05
24
Rensen et al.
Devlopment and Evaluation of a Real-
2005 Foodborne Pathogens and
Disease 2(2)152-159
Time Fluorescent Polymerase Chain
FTA was used to detect Bovine Meat and Bone Meal
(BMBM) contaminants in two kinds of cattle feed.
Reaction Assay for the Detection of
10gram samples of BMBM spiked feed were extracted
Bovine Contaminates in Cattle Feed.
with a lysis buffer and after extraction the lysis
cattle feed, bovne,
contaminants, ruminant
protein, runinant byproducts
buffer was spotted to FTA cards. Disks, 2mm, were
punched and treated with DNA-free RNase to increase
the sensitivity of the PCR. The DNA was extracted
from the punch using Instagene (Chelex) matrix.
Extracted DNA was used in real time PCR
amplifications. The authors say this method employing
FTA can be easily automated for routine screening.
Renshaw et al.
Amplification of DNA from blood
2001
Focus 23: 8-9
2000
Human Mutation 16:77-85.
spots: the importance of collection
paper & primer sequence
Roberts et al.
Rapid and Comprehensive
Determination of Cytochrome P450
CYP2D6 Poor Metabolizer Genotypes
by Multiplex Polymerase Chain
Reaction.
Rogers &
Reverse Transcription of an RNA
Burgoyne
Genome from Databasing Paper
(FTA)
2000 Biotechnology Applied
Biochemistry 31:219-224
The yield of PCR product using DNA on FTA as a template Factor V Leiden,
was twice as high as when using DNA on Guthrie cards as methylenetetraa template for Factor V amplification.
hydrofilate reductase,
MTHFR, polymorphism,
thromboembolic, disease,
Guthrie cards, blood,
human, infant,
Peripheral blood was either applied to FTA cards or DNA
ARMS assay, SNP,
extracted by the following methods, guanidine
multiplex, clinical study,
isothiocyanate, sodium hydroxide boiling lysis, proteinase long PCR, alleles, hotK/phenol-chloroform or salting out. FTA punches or
start PCR.
extracted DNA was then analyzed in a multiplex PCR
assay to detect different forms (alleles) of the CYP2D6
gene. The ARMS assay used here is a multiplex PCR
assay to detect several alleles at a time and is conducted
from a >4Kb DNA fragment amplified from genomic DNA.
FTA was a suitable template to generate the >4Kb starting
material for the ARMS assay. The authors found that a hotstart PCR was essential when using FTA punches.
Archiving of RNA genomes at room temperature using
FTA. Detecting RNA in transfusion blood samples.
Different methods tried to keep RNA on filter.
FTA Reference DatabaseJun05
Coxsackievirus, CVB-4,
RT-PCR, virus,
diagnostics, immobilized
RNA, viral, transport,
safety, enterovirus,
25
Rogers &
Bacterial Typing: Storing and
Burgoyne
Processing of Stabilized Reference
1997 Analytical Biochemistry 247:
223-227
Illustrates FTA for collecting, storing, rapidly analyze
microorganisms.
Bacteria for PCR Without Preparing
DNA-An Example of an Automated
Procedure
Rogers et al.
Development of a Buccal Swab Kit
1997 Poster: 8th Annual Int.
for the Collection of DNA Database
Symposium of Human
Samples
Identification Scottsdale,
Use of a buccal collection kit for DNA analysis.
16S, 23S, ribosomal RNA
genes, archiving,
ribotyping,
Staphylococcus, E. coli,
automation, genetic ID,
infections, pathogenic,
clinical isolates,
offender specimens,
buccal, foam tipped
applicator, training
AZ, USA
Rogers et al.
Implementation of a Buccal Swab Kit
for the Collection of DNA Database
Samples
Salvador & De
Isolation of DNA from Saliva of
Ungria
Betel Quid Chewers Using Treated
Cards.
Salvador JM
DNA Stability of Forensic STR
and MCA De
Markers in FTA™ Extracted Buccal
Ungria
DNA of Betel-quid Chewers.
Sauerbrei et al. Genetic Profile of an Oka Varicella
Vaccine Virus Variant Isolated from
an Infant with Zoster.
1998 Poster: 9th Annual Int. Symp. Buccal sample processing on FTA for STR analysisPotential PCR inhibitors in saliva don't reduce efficiency of
on Human Identification
PCR on FTA.
Orlando, FL, USA
DNA database, buccal,
DNA typing,
contaminants, training,
beverages, candy,
tobacco, food, lipstick,
mouthwash,
2003 J Forensic Sci 48(4)749-797 FTA used to collect buccal cells for DNA analysis from
people who chew betel quid. Betel quid contains DNA
damaging agents and PCR inhibitors. FTA very
successfully isolated the DNA away from the agents and
inhibitors to allow PCR amplification.
plant, human,
polyphenolics, PCR
inhibitors, STR, field
collection, genetic studies
2004 Oral Oncology 40:231-232.
Buccal cell DNA from betel quid chewers may be unstable buccal cells, genetic ID,
as determined by dropout of STR loci when cell samples
human ID, plant extract
are collected by traditional methods and DNA isolated by
organic extraction. Buccal cells collected and applied to
FTA did not show this instability suggesting that the
organic extracted DNA contained an agent that degraded
the DNA.
2004 J. Clin. Microbiol.
Varicella virus causes chickenpox. People vaccinated
multiple PCRs, pure DNA
against chicken pox with an inactivated viral solution can
stored on FTA, viral DNA
sometimes come down with shingles (zoster). DNA from
detection, DNA viruses
blood samples was purified with Qiagen Easy DNA kit or
QIAamp blood kit. Purified DNA was stored on FTA at
room temp for up to 1 month before PCR. 4mm squares of
FTA were washed as normal and used at least three times
in PCRs for multiple SNP detections. Approx 100 ng of
DNA were on the filter pieces.
42(12)35604-5608.
FTA Reference DatabaseJun05
26
Schifferli et al. DNA Obtention to Study BLAD in
Argentine and Spanish Cattle.
Schulman et al.
Microsatellite Analysis of
Cryopreserved Stallion Semen
2000 Arch. Zootec. 49:505-508.
(in spanish!)
2002 J South African Vet Assoc
73(4):222-223.
Stored on FTA® Paper.
Seabury, CM
Identification of a novel ovine PrP
and JN Derr
polymorphism and scrapie-resistant
2003 Cytogenet Genome Res
102:85-88.
genotypes for St. Croix White and a
related composite breed.
Seah &
DNA Archiving on FTA Paper
Burgoyne
Photosensitizer Initiated Attacks as
Models of Aging
Animal biotechnology,
blood, diagnostic,
breeding stock.
Use of FTA to sample sperm and blood from stallions for
genetic ID matching. Processed sperm (concentrated by
centrifugation and most seminal fluid removed) diluted 1:10
or 1:100 put on FTA. DDT and Proteinase K added to FTA
purification reagent to lyse sperm before multiplex PCR.
horse, frozen, blood,
sperm, microsatellites,
genetic screening,
artificial insemination
Whole blood collected from sheep onto FTA for PCR
analysis and the amplified DNA was sequenced to look for
DNA base changes. A 1.2mm punch was used in a 25ml
PCR. A new DNA polymorphism may help to determine if
some sheep are resistant to developing scrapie.
sheep, blood, animal
biotechnology,
polymorphism, disease
resistance
2000 Poster 4th HUGO Pacific
Describes microgel electrophoresis of plasmid DNA from
FTA. Studies of FTA stabilizing DNA in accelerated aging
Meeting and 5th Asia-Pacific
treatments.
Conference on Human
DNA stability, aging,
strand breaks, riboflavin,
hematoporphyrin, free
radicals,
Genetics
Seah &
DNA Databasing on FTA® Paper:
Burgoyne
Biological Assault and Techniques for
2000 Progress in Forensic
Genetics 8:74 - 77
Measuring Photogenic Damage
Describes the microgel electrophoresis technique to
measure integrity of DNA on FTA after attempted
damage from accelerated ageing (free radicals, UV and
mould attack)
Seah &
DNA Databasing on FTA Paper:
2000 Poster International Society
Burgoyne
Biological Assault and Techniques for
for Forensic Haemogenetics
Measuring Photogenic Damage
1193
Seah &
The Study of DNA Integrity on
1998 Poster 14th International
Burgoyne
Databasing Material: an In-situ
Symposium on Forensic
Electrophoretic Method
Sciences. The Francois
Vidocq Conf Oct 1998.
Illustrates the ability of FTA to prevent damage of
stored nucleic acid by UV
Used an in-situ electrophoretic technique to determine
DNA damage over time, and under different storage
conditions for DNA stored on plain paper and FTA Cards.
The DNA on FTA showed less damage and no fungal
growth when compared to plain paper.
FTA Reference DatabaseJun05
DNA stability, aging,
strand breaks, riboflavin,
hematoporphyrin, free
radicals, humid
conditions,
DNA stability, aging,
strand breaks, riboflavin,
hematoporphyrin, free
radicals, humid
conditions,
DNA stability, high
humidity, ambient
storage, blood, buccal,
27
Seah &
Photosensitizer initiated Attacks on
2001 J. Photochem and Photobiol
Burgoyne
DNA Under Dry Conditions and their
B: Biology 61: 10 -20.
Inhibition: a DNA Archiving Issue.
Free radical reactive ions which normally damage DNA
DNA damage, dry DNA
were artificially generated to "damage" plasmid DNA on
FTA. This paper studies how FTA protects DNA from
the damage and tries to quantitate the damage using
microgel electrophoresis.
Seah et al.
STR Data for the AmpFL STR
Identifiler loci in three ethnic
2003 Forensic Science
International 138:134-137.
groups (Malay, Chinese, Indian) of
the Malasian population.
da Silva et al.
Visceral leishmaniasis Caused by
Leishmania (Viannia) braziliensis in a
punch.
2002 Rev. Inst. Med. Trop. S. Paulo Patient blood and bone marrow collected onto FTA.
parasite, co-infections,
blood, bone marrow,
After washing punch containg purified DNA was
diagnostic, diagnosis,
amplified by PCR to detect Leishmania . Reference
clinical samples,
strains were applied to FTA and purified DNA amplified environmental
44(3) 145-149.
Patient Infected with Human
Immunodeficiency Virus.
da Silva et al.
Diagnosis of Human Visceral
Leishmaniasis by PCR using Blood
Blood was collected from 638 people onto FTA. Punches blood, human id, allele
frequencies, population
(1.2mm) were taken and washed. The 15 STR loci in the
study
Identifiler kit were amplified directly from the FTA
2004 Genetics and Molecular Res.
3(2): 251-257
as positive controls.
Blood and bone marrow aspirates as a volume of 30ul
parasites, bone marrow,
was applied to FTA cards and washed as usual prior to
Samples Spotted on Filter Paper
PCR amplification. Using molecular techniques visceral
leishmaniasis can be diagnosed instead of using
microscopic examination or immunoassays for the
Sipes et al.
FTA® Beyond the Everyday: Novel
FTA Applications to Improve
2000 Poster: 11th International
Symposium on Human ID
Efficiency.
Sippel &
Forensic Casework Experience:
Sajantila
FTA® Bloodstain Card as a Matrix
2000 Poster Promega 11th Annual
evidence of parasites .
DNA stored on non-FTA coated paper can be processed
throughput, DNA extract,
for amplification using the FTA procedure but high MW
static charge,
DNA is degraded and the yield is low. Freezing punches in
FTA Purification Reagent gave poor results. It is not
necessary to let the punch dry completely after the last
wash but all wash must be removed.
FTA is useful in collecting samples from autopsy cases
decomposition, STR,
QiAquick, human ID
Pneumocystis jirovecci is a fungus causing pneumonia in
immunosuppressed individuals. Approx 14ml of
bronchioalveolar lavage or sputum was applied to a 6 mm
disk of FTA. One fourth of the disk was washed with FTA
purification reagent followed by TE-1 then dried at 80oC.
The washed FTA was used directly in a 50ml PCR
amplification.
Fungus, clinical samples,
disease diagnostics.
Int Symp Of Human ID.
for Blood Samples from Fresh and
Decomposed Bodies.
Siripattanapipon Genotype Study of Pneumosystis
g et al.
jirovecii in Human Immunodeficiency
Virus-positive Patients in Thailand
2005 J Clin Microbiol 43(5):21042110.
FTA Reference DatabaseJun05
28
Sitaraman et al. Amplification of Large DNA from
1999 Focus 21(1):10
PCR fragments of 4.1, 5.2, 7.5, 8.0 and 8.4 kb amplified
from FTA punches containing blood
human, blood, large PCR
fragments, Taq, Platinum
Taq, Platinum Taq High
Fidelity
2001 Gene 271: 273-283
Species identity study used FTA paper to collect, ship,
blood, birds, chicken, cat,
horse, cow, rat, lizard,
tissue, PCR, cloning,
DNA sequencing,
Blood Stored at Room Temperature
Smith &
Species identity: conserved inverted
Burgoyne
LINE repeat clusters (ILRC) in the
store and analyze DNA from several species of birds,
vertebrate genome as indicators of
mammals and amphibians. DNA from birds and lizard
population boundaries
was heat eluted and soluble DNA used in PCR.
Smith, LM and
Collecting, archiving and processing
Burgoyne, LA
DNA from wildlife samples using
2004 BMC Ecology 2004, 4:4
(Article available at http://www.biomedcentral.com/
2002 Poster 13th International
New advances for FTA technology.
PCR, DNA extraction,
DNA IQ, hair samples,
automation, 96 well FTA
format
Human urine was spotted on FTA cards then amplified by
PCR using the Profiler Plus and Cofiler kits.
nuclear DNA,
mitochondrial DNA, male,
female, STR, PCR,
FTA® databasing paper.
Smith et al .
FTA® Technology – New Advances
for the Collection, Shipment,
horse, chicken, cow,
crested pigeon
1472-6785/4/4). A variety of wildlife sample types
(Geophaps lophotes) and
were collected and processed on FTA for PCR analysis. sleepy lizard (Tiliqua
Blood samples, blood clots, tissue, saliva and buccal cells rugiosa), pelicans, mallee
fowl (Leipoa
samples were applied to FTA. Several variations of
ocellata), frog (Rana sp.),
processing FTA disks are described. Techniques for
yabbie (an Australian
nucleated avian red blood cells are described. Methods fresh water crustacean,
Cherax tenuimanus ),
to elute DNA from FTA are described.
abalone (Haliotis
laevigata ) and blue
swimmer crab (Portunus
pelagicus), King George
Whiting (Sillaginodes
punctata ) and tuna
(Thunnus maccoyii)
Symposium on Human ID
Archiving and Processing of Biological
Samples for Human Identification
Smuts & Pogue
DNA from Urine as a Potential
Source of Identification
1999 Poster 10th International
Symposium on Human ID
FTA Reference DatabaseJun05
29
Snowden et al.
Simple, Filter-based PCR Detection
2002 Journal of Eukaryotic
of Thelohania solenopsae
Microbiology, 49(6): 447-
(Microspora) in Fire Ants
448.
(Solenopsis invicta
Subrungruang
Evaluation of DNA Extraction and
et al.
PCR Methods for Detection of
Enterocytozoon bienuesi in Stool
Specimens
Sullivan
Univ. Los Angeles, CA
IsoCode Sample Registration Matrix
cards and FTA cards and Filter
Matrix Bloodstain cards.
Taback et al.
spores, microsporidian,
parasite, bead beating
homogenate, ssu rRNA
gene,
Feces, fecal, parasite,
2004 J Clin. Microbiol 42(8)3490- Three methods to extract DNA from stool samples were
compared; FTA, QIAamp stool mini and chloroform/phenol diagnostic, protozoan
3494
extraction. FTA and QIAamp more sensitive than ch/ph.
FTA was chosen due to ease, high sensitivity, low cost and
less amount of sample required compared to QIAamp kit.
Comparison Study of DNA extraction 2003 MA Thesis California State
and analysis from IsoCode cards,
Application of ant/parasite homogenate to FTA Cards, and
comparing that to the conventional methods of extraction.
They were looking for a microsporidian parasite in fire ants.
20 ul of ant homogenate on FTA FTA detected fewer
spores than silica based DNA purification.
Prognostic Significance of Circulating 2001 Cancer Res. 61:5723-5726.
Microsatellite Markers in the Plasma
Post mortem blood from autopsies applied to FTA,
Whatman BloodStain cards and IsoCode cards. Buccal
cells from volunteers applied to IsoCode Sample
Registration Matrix. DNA was extracted from all media by
chelex. Chelex was significantly better than IsoCode's
DNA extraction protocol with 95oC in water. IsoCode was
comparable in number of STR identifed compared to
samples on FTA and Bloodstain Cards. Study was done to
initially validate IsoCode for the Medical Examiners Office.
IsoCode, S&S,
registration matrix,
chelex, extracted DNA,
blood, buccal, postmortem samples
FTA used to collect blood from control patients in clinical
study.
human, diagnostic,
cancer study,
Whole blood was applied to FTA as a source of control
genomic DNA. Portions of genes involved in tumor
suppression and metastasis were analyzed from control
and tumor derived DNA samples by PCR.
human, diagnostic,
cancer study,
One full or a half 1.2mm discs amplified in 5 and 10ml
Powerplex 1.2, chelex,
AmpFƒSTR Blue,
of Melanoma Patients.
Taback et al.
Detection of Tumor-Specific Genetic 2003 Cancer Res. 63:1884-1887.
Alterations in Bone Marrow from
Early-Stage Breast Cancer Patients.
Thacker et al.
Use of FTA Cards in Small Volume
PCR Reactions
1999 Progress in Forensic
Genetics 8, 473-475.
PCR mixes. Compare to chelex extracted DNA. Discs
washed in a flat bottom multiwell plate on a shaker
using 3 x 100ml FTA Purification Reagent and 2 x 100 ml
TE washes.
FTA Reference DatabaseJun05
30
Thompson et al. Congenital myasthenic syndrome of
2003 Vet. Rec. 153(4): 779-781.
Brahman cattle in South Africa
Cattle blood (from EDTA vacutainer) and semen were
applied to FTA cards. Punches of 2mm were PCR
amplified in 25ml. Semen was diluted 1:10 before
Breeding cattle,
diagnostic, animal
biotechnology, gene
deletion, screening test
application. Punches with semen were resuspended in
200ml FTA Reagent, 20ml 1M dithiothreitol and 5ml
20mg/ml Proteinase K for 1 hr at 56oC then washed
with FTA Reagent and TE-1 as usual before PCR.
Tilsala-
Rapid DNA preparation from milk and 2004 Food Microbiol, 21:365-368
Timisjarvi and
dairy process samples for the
Alatossava
detection of bacteria by PCR.
Liquid dairy samples or homogenized solid diary samples bacteria, environmental,
milk, cheese, yogurt,
were applied as 20 - 40 ml to FTA. Two 2 mm punches
clinical mastitis milk
were taken washed and amplified directly by PCR for
samples, dairy st
the detection of probiotic and pathogenic microbes.
"…the FTA method is easier to perform and ...doesn't
not include the use of volatile solvents or other toxic
Tsukaya H
A Simple Method for Collecting DNA
Samples in the Field
2003 Newsletter of Himalayan
Botany 32:15-17.
reagents."
Describes FTA as the most convenient method of
collecting plant specimens in the field. Compared FTA
to Sigma REDEXtract-N-Amp Plant kits on the basis of
Plant samples, phenol,
polysaccharides, ExtractN-Amp
room temp stability of DNA and no centrifugation.
Leaves from several plant species, common weeds,
succulent plants, woody plants and one with a high
polysaccharide content (a problem for DNA isolation)
were collected with both kits. Author washed the
whole card then took a 1 x 2 mm segment for PCR in 50
ul reaction. Efficiency of PCR with FTA was better
than Sigma kit but Sigma kit was more precise (less non
specific junk bands on gel). The Sigma kit did not work
with plants with high polysaccharides or phenolic
compounds. FTA worked well with all samples. "FTA
Cards are highly recommended for DNA collection in
the field....".
Tsukaya H
Gene Flow Between Impatients
Radicans and I. Javensis
(Balsaminaceae) in Gunung Pangrango,
2004 American Journal of Botany
91(12)2119-2123
FTA cards were used to collect leaf presses from the
plant, leaf presses, PCR
plants. The whole card was washed and a 1 x 2mm
section was taken for PCR amplification in 50ul
Central Java, Indonesia
FTA Reference DatabaseJun05
31
Tsukaya et al
Large-Scale general collection of wild- 2005 J Plant Res. Online First
Project to collect 355 herbarium samples and DNA
plant DNA on Mustang, Nepal
specimens from Nepal to be included in the Flora of
plant, collections, PCR,
DNA database
Nepal Database website. Plant leaves were pressed
onto FTA, washed and 1mm2 sections were amplified by
PCR. The authors drew a grid on a classic card to store
20 samples/card and found no cross contamination. The
Flora data base may be using FTA to send DNA samples
out to researchers.
Vanek et al.
Czech population data on 10 short
tandem repeat loci of SGM Plus STR
2000 Forensic Science
Population study on 10 STR loci on 202 unrelated Czech
Caucasians.
Population study, blood,
human ID
2004 Gut 53:871-876.
FTA was used to collect whole blood samples from
patients with cancer and normal control patients. Three 2
mm disks were washed with FTA reagent then TE-1
followed by drying at 60oC for 30 min. Portions of the
alcohol dehydrogenase gene were amplified by PCR then
digested for RFLP analysis.
population study, blood,
human ID, allele
frequency, cancer
predisposition
2003 African Journal of Ecology
Reference blood samples from monitor lizards and tsetse
smears collected onto FTA. This publication's main
analysis was ELISA to discriminate tsetse species. No
DNA work was presented.
International 119:107-108
system kit using DNA Purified in
FTA cards
Visapää et al.
Increased cancer risk in heavy
drinkers with the alcohol
dehydrogenase 1C*1 allele, possibly
due to salivary acetaldehyde.
Waiswa et al.
Monitor lizard (Varanus niloticus,
Linnaeus, 1766) as a host for tsetse
41,349-351.
(Glossina fuscipes fuscipes,
Newstead, 1910) in the sleeping
sickness endemic foci of Uganda.
Wang et al.
SNP Analysis Based on Strand
2004 IVD Technol. 10(6)61-71
Work from BD Diagnostics describing BD ProbeTec ET
diagnostics, WGA,
system that looks at SNPs via a whole genome
amplification approach instead of other SNP methods like
primer extension or microarray. Blood on FTA serves as a
good DNA template for this platform. Paper can be
accessed at
www.devicelink.com/ivdt/archive/04/07/012.html
1996 Poster Advances in Forensic
Illustrates the Rosys GeneMachine for the processing of
automation, liquid
DNA on FTA cards for STR profiling. FTA superior to 903. handling, blood, STR,
903 v FTA
Displacement Amplification
Williams et al.
Automation of in situ Sample
Preparation for PCR Using the FTA
DNA Collection System and the
Haemogenetics 6
Rosys Laboratory Workstation
FTA Reference DatabaseJun05
32
Yet et al.
Comparison of DNA Recovered from
1997 Poster: 8th Annual Int.
FTA™ Paper to Conventional Organic
Symposium of Human
Extraction Procedures using a Hae
Identification
DNA collected and archived on FTA performed well for
RFLP analysis.
RFLP, archiving,
preservation
III Based RFLP System.
Zhong et al.
Comparison of IsoCode STX and FTA
Gene Guard Collection Matrices as
2001 Journal of Clinical
Microbiology 39: 1195-1196
Whole Blood Storage and Processing
Devices for the Diagnosis of Malaria
by PCR
Zhou and
Allelic Polymorphism in the Ovine
Hickford
DQA1 gene
Zhou H,
Technical Note: Dtermination of
Hickford JGH
alleles of the ovine PRNP gene using
and Fang Q
PCR- single-strand conformational
2004 J. Anim Sci. 82:8-16
Blood from 300 sheep was collected using FTA cards. The genetic analysis,
gene for DQA1 was amplified by PCR and sequenced.
agriculture, animal,
2005 J. Anim. Sci 83:745-749.
Blood from 400 sheep covering 6 breeds was collected
onto FTA cards to amplify a 173bp fragment of the prion
gene. Codons 136, 154 and 171 are tested to determine
an animal's susceptibility to scrapie. Punches of 1.2mm
were included in the 20µl PCR mix. The selected
amplicons were inserted into plasmids and sequenced.
sheep, SSCP, rapid
genotyping method, prion
protein gene
2005 J. Anim Sci 83:963-968.
Blood samples were collected from Boer goats onto FTA.
Punches, 1.2mm, were washed according to the standard
protocol and included in 20µl PCR amplifications.
caprine, goats, single
stranded conformational
polymorphism, SSCP,
cloning, sequencing
polymorphism analysis.
Zhou H,
Polymorphism of the DQA2 gene in
Hickford JGH
goats
and Fang Q
FTA outperforms IsoCode by >20% for the detection of
diagnosis, transportation,
multiple species malaria… 96% detection of single species parasites, plasmodial
malaria.
small subunit RNA gene,
Plasmodium vivax , single
species infections, mixed
infections
FTA Reference DatabaseJun05
33
FTA® Blood
DNA Whatman
Raccolta, trasferimento,
stoccaggio e purificazione
di DNA da campioni di
sangue
FTA della Whatman offre un valido e semplice metodo per la
preparazione di campioni per analisi di DNA da sangue.
Occorre, infatti, semplicemente applicare un campione di
sangue, midollo osseo o buffy coat alla matrice FTA. Il DNA
viene catturato e stabilizzato all’istante, consentendone lo
stoccaggio per un periodo indefinito a temperatura
ambiente, da analizzare in qualsiasi momento. Trasporto
sicuro e purificazione in 30 minuti rendono la tecnologia FTA
un indispensabile strumento di ricerca.
Caratteristiche Principali
• Semplice raccolta dei campioni
I campioni vengono depositati
direttamente sulla matrice FTA.
• Purificazione rapida FTA
rappresenta il metodo più veloce
per la purificazione di DNA da
campioni di sangue per PCR. Il DNA
viene purificato sulla FTA Card in
tre semplici steps, in un singolo
tubo, a temperatura ambiente. Non
sono necessarie sostanze chimiche
tossiche, inoltre rimanendo il DNA
fissato sulla matrice è subito pronto
per la PCR.
• Adatte a qualsiasi metodo Le FTA
Cards si adattano alle diverse
tipologie di campione: preparazione
di campioni di sangue, midollo
osseo o buffy coat. E’ sufficiente
applicare direttamente i campioni
sulla matrice FTA e non è necessaria
alcuna ulteriore purificazione nè
con sostanze tossiche (metodi quali
fenolo/cloroformio) nè con altri
metodi che possono richiedere
lunghe incubazioni.
• Stoccaggio a temperatura ambiente
e trasferimento sicuri Gli acidi
nucleici vengono automaticamente
stabilizzati senza la necessità di
congelarli. FTA inattiva gli agenti
patogeni potenzialmente nocivi
rendendo i campioni sicuri per la
loro manipolazione in laboratorio.
Inviare i campioni a colleghi o al
laboratorio centrale è veramente
facile con FTA Cards. Occorre solo
inviarli per posta!
• Automatizzabile L’utilizzo di sistemi
automatici accelera le fasi di
preparazione di diversi dischi
prelevati contemporaneamente,
ottimizza le risorse disponibili e
standardizza la purificazione di
DNA. I dischi prelevati dalla FTA
Card possono essere sottoposti alle
fasi di lavaggio e preparazione per
la PCR su una diversi tipi di
strumenti presenti in commercio
(liquid handling robot).
Applicazioni
• Applicazioni diagnostiche e
cliniche
• Tipizzazione tissutale HLA
• Identificazione genetica
• Studi di screening molecolari
• Studi di allevamento
• Whole genomic amplification
TRE SEMPLICI PASSAGGI
OPERATIVI PER OTTENERE UN
DNA PURO
Dispensare il
campione
Purificare
Prelevare
un piccolo
disco
(punch) dal
Campione
PROTOCOLLO APPLICATIVO DEL FTA BLOOD DNA
Dispensare campione
Dispensare il campione
di sangue, midollo osseo
o buffy coat sulla FTA
Card. Lasciarlo asciugare
completamente.
Lavaggio con soluzione
tampone TE-1
Effettuare due lavaggi con
la soluzione tampone TE-1
(10mM Tris, 0,1 mM EDTA,
pH 8,0). Eliminare la
soluzione usata dopo
ciascun lavaggio.
Prelevare un piccolo
disco
Prelevare (mediante
perforazione) un piccolo
disco dal campione
depositato sulla FTA
Card.
Essiccazione
Lasciar asciugare il disco nel
tubo PCR
Purificazione con
Reagente di Purificazione
FTA (Purification Reagent
FTA)
Mettere il disco di
campione in un tubo per
PCR ed effettuare tre
lavaggi con il Reagente
di Purificazione FTA.
Eliminare la soluzione
utilizzata dopo ciascun
lavaggio.
PCR
Aggiungere la master mix
per la PCR direttamente al
disco e iniziare la reazione
di amplificazione.
Qualità Whatman
Whatman, azienda leader nelle teconologie di seprazione, è
nota nel settore scientifico per prodotti e soluzioni innovative
contraddistinte da un’elevata qualità. La certezza del valore di
una sempre maggior semplificazione delle procedure di
laboratorio tende ad accellerare lo sviluppo di nuovi prodotti,
a ridurre i costi e tempi di processo. Inoltre, per accentuare
ulteriormente l’impegno nel soddisfare esigenze specifiche dei
consumatori, Whatman è organizzata in quattro aree distinte:
Chimica Analitica, Diagnostica, Genomics e Proteomica, e
Dispositivi Medicali. Per ulteriori informazioni vogliate
collegarvi al sito www.whatman.com.
FTA, GenSpin e Whatman sono marchi registrati della
Whatman Group.
INFORMAZIONI PER L’ORDINE DAL CATALOGO WHATMAN
No. Cat.
Descrizione
Quantità per
Confezione
WB120061
WB120204
WB120205
WB120055
WB120210
WB120208
WB100005
WB100006
WB100028
WB100010
WB100011
WB120216
WB120217
WB100025
WB100003
WB100016
WB100020
WB100030
WB120005
FTA Starter Pack
Reagente di Purificazione FTA
FTA Classic Card (non-indicatrice)
FTA Mini Card (non-indicatrice)
FTA Micro Card (non-indicatrice)
FTA Gene Card
Harris Micro Perforatore 1.2mm
Harris Micro Perforatore 1.2mm Tip
Harris Uni-Core 1.25mm Perforatore
Busta Multi-Barrier (grande)
Busta Multi-Barrier (piccola)
FTA Gene Card/Tasca/Disseccante
FTA Classic Card/Tasca/Disseccante
1.2mm Plunger di Ricambio
Essiccante (1g)
Busta Postale per FTA Card
Tagliere di Ricambio
Supporto per FTA Gene Card
GenSpin Kit di Purificazione DNA
1
500mL
100
100
100
100
1
1
4
500
500
1000
1000
1
1000
50
1
20
50 Purificazioni
Purificazione del DNA in meno di 25
minuti! Il GenSpin Kit di Purificazione del
DNA della Whatman rappresenta un
metodo estremamente semplice per
purificare il DNA a singolo filamento da
campioni di sangue e cellule in coltura. Il
campione è subito pronto per la PCR a
partire anche da piccoli volumi del
campione di partenza.
North America Whatman Inc
9 Bridewell Place
Clifton, NJ 07014 USA
Technical Support: 1-800-922-0361
Customer Service: 1-800-631-7290
Outside US: 973-773-5800
Fax: 973-773-0168
E-mail: [email protected]
Europe Whatman International Ltd
Springfield Mill
James Whatman Way
Maidstone
Kent ME14 2LE UK
Tel: +44 (0)1622 676670
Fax: +44 (0)1622 677011
E-mail: [email protected]
Japan Whatman Japan KK
Daiwa Ueno Building 1F
6-10 Ueno 5-chome, Taito-ku
Tokyo 110-0005, Japan
Tel: +81 (0)3 3832 6707
Fax: +81 (0)3 3832 6457
E-mail: [email protected]
Asia Pacific Whatman Asia Pacific Pte Ltd
171 Chin Swee Road
#08-01 San Centre
Singapore 169877
Tel: +65 6534 0138
Fax: +65 6534 2166
E-mail: [email protected]
Whatman GenSpin™ Kit di Purificazione DNA
Leaders in Separations Technology
www.whatman.com
Cat No. S9036-779
FTA® Total RNA
Whatman
Raccolta,
trasferimento,
stoccaggio e
purificazione di
RNA Totale
FTA della Whatman offre un valido e semplice metodo per la
preparazione di RNA Totale da una varietà di tipologia di
campioni di partenza per analisi molecolari. Occorre, infatti,
semplicemente applicare un campione di sangue, di cellule in
coltura, o pressare foglie sulla matrice FTA. Gli acidi nucleici
sono catturati e stabilizzati all’istante, mentre agenti
patogeni e nucleasi vengono inattivati. Il trasferimento e lo
stoccaggio dei campioni sono sicuri fino al momento
dell’analisi. Purificazione di RNA per RT-PCR risulta
estremamente semplificata. Provate FTA e lo troverete
immediatamente un indispensabile strumento per il
laboratorio di Biologia Molecolare.
Tre semplici passaggi
operativi per RNA Totale
Caratteristiche Principali
• RNA adatto per TranscriptasiInversa (RT)-PCR e Northern
Blotting RNA purificato da FTA
può essere utilizzato come stampo
nelle analisi RT-PCR direttamente in
soluzione, oppure può essere
precipitato e caricato su gel di
agarosio per analisi di Northern
Blotting.
• Raccolta I campioni sono applicati
direttamente sulla matrice FTA. Gli
acidi nucleici vengono
automaticamente stabilizzati e
protetti: per un breve stoccaggio a
temperatura ambiente e, per
periodi più estesi di stoccaggio, a
temperature di -20ºC o -70ºC.
• Stoccaggio a temperatura
ambiente e trasferimento sicuri
FTA inattiva agenti patogeni nocivi
rendendo i campioni sicuri per la
manipolazione in laboratorio e per
il trasferimento.
• Rapida purificazione RNA viene
eluito interamente dalla FTA Card
mediante un semplice step di
lavaggio e dispensato in un singolo
tubo, a temperatura ambiente. Non
richiede l’utilizzo di sostanze
chimiche tossiche. RNA viene eluito
dalla matrice ed è subito pronto per
reazioni di RT-PCR.
• Adatto al vostro metodo Non
richiede ulteriori procedure di
purificazione con lunghi metodi
quali fenolo/cloroformio, fasi di
digestione o disgregazione di
tessuti.
Applicazioni
• Applicazioni di ricerca
— RT/PCR
— Northern Blotting
• Espressione genica
• PCR ‘real time’
Dispensare il Campione
Prelevare
mediante
perforazione
un piccolo
disco (punch)
dal Campione
Purificare
PROTOCOLLO APPLICATIVO DEL FTA TOTAL RNA
Dispensare Campione
Dispensare il campione sulla
FTA Card. Lasciarlo asciugare
completamente.
Eluizione del RNA con
Soluzione Tampone
Aggiungere la soluzione
tampone, incubare in
ghiaccio.
Prelevare un piccolo disco
Prelevare mediante
perforazione un piccolo
disco (punch) dal Campione
sulla FTA Card e inserirlo in
un tubo da 1,5mL Perforare
estraendo un disco dal
campione sulla FTA Card e
inserirlo in un tubo da
1,5mL.
RT-PCR o Northern
Blotting
Usare RNA direttamente per
RT-PCR oppure precipitare
per analisi Nothern Blot.
RT-PCR da FTA: RNA da cellule HL60 estratte con FTA o con metodo Competitor T
MW
1
2
3
4
5
Qualità Whatman
Whatman, azienda leader nelle tecnologie per la separazione,
è nota nellà comunità scientifica per prodotti e soluzioni
innovative contraddistinte da un’elevata qualità. Il nostro
istinto per la semplificazione accelera la velocità di fare nuove
scoperte, riduce i costi e risparmia tempo. Inoltre, per
accentuare ulteriormente l’impegno nel soddisfare le
specifiche esigenze dei nostri clienti, Whatman è organizzata
in quattro aree distinte: LabScience, BioScience, MedTech
Diagnostica e MedTech Dispoitivi. Ulteriori informazioni sono
disponibili collegandovi al sito www.whatman.com.
6
FTA, GenSpin e Whatman sono marchi registrati della
Whatman Group.
North America Whatman Inc
9 Bridewell Place,
Clifton, NJ 07014
Technical Support: 1-800-922-0361
Customer Service: 1-800-631-7290
Outside US: 973-773-5800
Fax: 973-773-0168
E-mail: [email protected]
Linea
Linea
Linea
Linea
Linea
1 & 2: PCR utilizzando RNA da FTA (nessuna contaminazione da DNA).
3:
Controllo Negativo (nessuno stampo).
4:
PCR utilizzando RNA da Competitor T (contaminazione DNA).
5:
PCR utilizzando DNA controllo positivo K562.
6:
RT-PCR utilizzando RNA da FTA.
INFORMAZIONI PER L’ORDINE DAL CATALOGO WHATMAN
No. Cat.
WB120061
WB120205
WB120206
WB120055
WB120056
WB120210
WB120211
WB120208
WB100007
WB100008
WB100029
WB100010
WB100011
WB120216
WB120217
WB100026
WB100003
WB100016
WB100020
WB100030
Descrizione
FTA Starter Pack
FTA Classic Card (non-indicatrice)
FTA Classic Card (indicatrice)
FTA Mini Card (non-indicatrice)
FTA Mini Card (indicatrice)
FTA Micro Card (non-indicatrice)
FTA Micro Card (indicatrice)
FTA Gene Card
Harris Micro Perforatore 2.0mm
Harris Micro Perforatore 2.0mm Tip
Harris Uni-Core 2.0mm Perforatore
Busta Multi-Barrier (grande)
Busta Multi-Barrier (piccola)
FTA Gene Card/Busta/Essiccante
FTA Classic Card/Busta/Essiccante
2.0mm Plunger di Ricambio
Essiccante (1g)
Busta Postale per FTA Card
Tagliere di Ricambio
Supporto per FTA Gene Card
Quantità
per Confezione
1
100
100
100
100
100
100
100
1
1
4
500
500
1000
1000
1
1000
50
1
20
Europe Whatman International Ltd
Springfield Mill
James Whatman Way
Maidstone
Kent ME14 2LE, UK
Tel: +44 (0)1622 676670
Fax: +44 (0)1622 691425
E-mail: [email protected]
Japan Whatman Japan KK
Daiwa Ueno Building 1F
6-10 Ueno 5-chome, Taito-ku
Tokyo 110-0005, Japan
Tel: +81 (0)3 3832 6707
Fax: +81 (0)3 3832 6457
E-mail: [email protected]
Asia Pacific Whatman Asia Pacific Pte Ltd
171 Chin Swee Road
#08-01 San Centre
Singapore 169877
Tel: +65 6534 0138
Fax: +65 6534 2166
E-mail: [email protected]
Leaders in Separations Technology
www.whatman.com
Cat No. S9036-785