Dipartimento di Chimica Biologia e Biotecnologie Workshop 2 dicembre 2015 ____________________________________________________________________________ Microbial ecology and applied microbiology Development and cell biology vs environment Conservation genetics and molecular ecology Population genetics and molecular evolution UNIT Development & cell biology vs environment (I. Di Rosa, A. Fagotti, F. Simoncelli, L. Barberini) Research lines : - Analysis of health status of sentinel organisms (planarians, mussels, amphibians) in response to environmental stress - Biomarkers of cellular stress in model organisms under natural and controlled conditions - Developing model organisms and chemical stress: potential applications in pharmacological and bio-medical fields current topic Biological responses of sentinel organisms to environmental stress Methods of analysis Amphibians as sentinel organisms Biphasic life cycle Soft, unscaly, permeable skin Ectothermic physiology Modes of reproduction and development Trophic importance Declines of populations and extinction of species Effective indicators of environment health and integrity Biological impact of environmental stress under natural and controlled conditions Biological responses (molecule, cell, organ, organism) to environmental stress Biodiversity monitoring and preservation Chytridiomycosis (chytrid fungus Batrachochytrium dendrobatidis) Simoncelli et al., 2005, EcoHealth Di Rosa et al., 2007, Nature Molecular identification of Batrachochytrium dendrobatidis by 18S SSU rDNA characterization 18S SSU rDNA di Batrachochytrium dendrobatidis 1 GGCCTACCATGGTGATAACGGGTAACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCT 61 GAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACAC 121 AGGGAGGTAGTGACAATAAATAACAAACCAGGGCTTTTATAGTTTTGGAATTGGAATGAG 181 TACAATTTAAATCTCTTAACGAGGAACAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCG 241 GTAATTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAACGCCCGTAGTTGAA 301 TGAGTGGACT TGTGCGGGATGGACCGCAAGAGAAAGTTTT TACGAGTTCAATATTTTGG 361 CAAGCA Simoncelli et al., 2005, EcoHealth Amphibiocystidiosis (mesomycetozoeans of the genus Amphibiocystidium) Di Rosa et al., 2015, submitted Amphibiocystidiosis (mesomycetozoeans of the genus Amphibiocystidium) Ep D Ip Ep D m CI Di Rosa et al., 2015, submitted Molecular identification of Amphibiocystidium by 18S SSU rDNA characterization 18S SSU rDNA di Amphibiocystidium 1 61 121 181 241 301 361 421 481 541 601 661 721 781 841 901 961 1021 1081 1141 1201 1261 1321 1381 1441 1501 1561 1621 1681 acaaatctat taccttacta ttctggaagg gtgattcata tctgccctat agaattaggg cagcaggcgc cagggcttta attggagggc aagttgttgc caaggtgttg ctgtggtgtg gcaggcgttt gttggtttct tgtcagaggt gatgttttca gtcctaacca gcaccttatg cttaaaggaa acacggggaa tgattctatg ttccgttaac tgacttctta gtgatgccct tcacctgtac ttgcaatttt attacgtccc aggtcttcag aacttgatca actgtgaaac cttggataac gatgtattta ataactttgc caactttcga ttcgattccg gcaaattacc taagtcttgt aagtctggtg agttaaaaag taccgagcca cgtccgtctt gcttgaatat aggaccaaag gaaattcttg ttaatcaaga taaactatgc agaaatcaaa ttgacggaag actcaccagg ggtggtggtg gaacgagacc gagggactat tagatgttct cggaaggtat cgatcttaaa tgccctttgt attggcgctt tttagaggaa tgcgaatggc cgtagtaatt ttagatagaa gaatcgtatg tggtaaggta gagagggagc caatcctgac aattggaatg ccagcagccg ctcgtagttg gccaatcctt atttcgggac ttcagcatgg taatgattaa gatttatgaa acgaaagtta cgactaggga gtctttgggt ggcaccacca tccagacatg catggccgtt ttaacctgct tggtgtttaa gggccgcacg gggtaatctt cgaggaattc acacaccgcc cgcagctggc gtaaaagtcg tcattaaatc ctagagctaa aaccaatgcg gctttgtgct ttggcttacc ctgagaaacg acagggaggt agtacaattt cggtaattcc gattttggga tcgcttctcg ttttactgtg aataatgaaa tagggatagt agactaactt ggggatcgaa ttggtagatg tccgggggga ggagtggagc gtaaggattg cttagttggt aaatagttgc ccaatggaag cgcgctacac ttcaaacttt ctagtaagcg cgtcgctact gacagcagtg taacaaggtt agttatagtt tacatgctaa ggcctcggtc gacgatgatt atggttacaa gctaccacat agtgacaaaa aaacccctta agctccaata tttggtcggt aaagcggcgt aaaaaattag taggacattg tgggggcatt ctgcgaaagc gatgatcaga ttaattctat gtatggtcgc ctgcggctta acagattgag ggagtgattt gtgattttcg tttgaggcaa tgataaaggc atcgtgctgg caagtcatca accgattgaa ggatgccgaa tatttgatag aaatcccgac cggttctttg cattcaaatt cgggtaacgg ctaaggaagg aataacaata acgaggaaca gcgtatatta tggtccgccg gtgcccttaa agtgttcaaa gttctatttt agtatttaat atttgccaag taccgtcgta gactctatca aaggctgaaa atttgactca agctctttct gtctggttaa aatcacttgg taacaggtct aacgagttta ggatagatct gcttgcgttg tggtttagtg aagttgatca Di Rosa et al., 2015, submitted Differences in microbial assemblages between frogs infected and uninfected by Amphibiocystidium Abundances of phylotypes identified as Pseudomonas, Flavobacterium, and Janthinobacterium in uninfected and infected specimens Skin organ culture and Cadmium-exposure assays + CdCl2 Stress response analysis Histomorphological modifications - Cd + Cd Tunel assay of untreated and Cd-treated skin organ culture - Cd + Cd mRNA expression profiles of Metallothionein and Heat shock proteins Mt HSPs Time-lagged correlation spectra of the annual meteorological data versus the seasonal data of water frog abundance (a,b) and fraction of P. bergeri (c,d) Time-lagged correlation spectra of the monthly meteorological data versus the seasonal data of water frog abundance (a,b) and fraction of P. bergeri (c,d)
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