Applied Microbiology and Biotechnology
Decolorization of acid and basic dyes: understanding the metabolic degradation and cellinduced adsorption/precipitation by Escherichia coli.
Matteo Cerboneschi,1* Massimo Corsi,2 Roberto Bianchini,2 Marco Bonanni,2 Stefania Tegli1
1
Dipartimento di Scienze delle Produzioni Agroalimentari e dell'Ambiente, Laboratorio di Patologia Vegetale
Molecolare, Università degli Studi di Firenze, Sesto Fiorentino (Firenze), Italy
2
Dipartimento di Chimica “Ugo Schiff”, Università degli Studi di Firenze, Sesto Fiorentino (Firenze), Italy
*Corresponding author: Matteo Cerboneschi, Dipartimento di Scienze delle Produzioni Agroalimentari e dell'Ambiente,
Laboratorio di Patologia Vegetale Molecolare, Università degli Studi di Firenze, Sesto Fiorentino (Firenze), Italy, Tel.
+39 055 4573228, FAX +39 055 4573232, e-mail: [email protected]
Fig. S1 Reductive cleavage of dye B46.
Fig. S2 Resonance hybrid of dye B46.
1
Fig. S3 Activity of living and dead cells of E. coli for dye decolorization after 16h of incubation Shaken living cells
(black), steady living cells (white) and shaken dead cells (grey).
Fig. S4 Inhibition profile of the decolorization rate of E. coli when inoculated with 0.15 mg/ml of B46 by different
carbon and nitrogen sources: 1% of glucose (circle), 0.5% of ammonium chloride (triangle) and 0.5% of ammonium
sulfate (square).
2
Table S1 Acute toxicity tests using D. magna on dyes crude and treated with E. coli. Dyes were bacterial-treated for 6,
12 and 24 hours. Untreated dyes (t = 0) were used as positive control. D. magna mortality was assessed after 48 h
treatment and tests were carried out in triplicate, using 25 living organisms.
Dyes
Time
Mortality (%)
A324
0
6h
12 h
24 h
0
6h
12 h
24 h
0
6h
12 h
24 h
0
6h
12 h
24 h
100  0
1.89  0.61
00
00
100  0
3.88  1.32
00
00
100  0
9.89  4.61
4.89  2.71
00
100  0
00
00
00
A361
B47
B46
3