Biotime di G. Niccolai e C. sas
Ghost Dyes™ and Cell Viability Reagents
Don't let dead cells haunt your Flow Cytometry data
Tonbo's Ghost Dyes™ & Cell Viability Reagents allow for clear live vs. dead cell discrimination for more precise flow
cytometry analysis, and are especially useful for intracellular staining protocols.
Ghost Dyes™ bind irreversibly to amine groups and are resistant to subsequent washing, fixation and
permeabilization. Dead or effete cells with compromised membranes allow Ghost Dyes™ to permeate and bind amine
groups of intracellular proteins resulting in fluorescence much brighter than live cells which are impermeant to Ghost
Dyes™
BIOTIME di G. Niccolai e C. sas
Via Salicotto, 143 – 53100 Siena
telefono 345 5180700
telefax 0577 221446
email [email protected]
website www.biotimenet.com
Codice Fiscale 00381250521
Partita IVA IT00381250521
Coordinate bancarie:
Banca Monte dei Paschi di Siena
IBAN: IT 54 W 01030 14200 0000 10648623
Biotime di G. Niccolai e C. sas
Choose from among the following Ghost Dyes™ and Cell Viability Dyes:
Product Name
Excitation Laser Emission (nm) Size
Ghost Dye™ Violet 450 405
450
100 tests
Ghost Dye™ Violet 450 405
450
500 tests
Ghost Dye™ Violet 510 405
510
100 tests
Ghost Dye™ Violet 510 405
510
500 tests
Ghost Dye™ Red 710 633
710
100 tests
Ghost Dye™ Red 710 633
710
500 tests
Ghost Dye™ Red 780 633
780
100 tests
Ghost Dye™ Red 780 633
780
500 tests
Propidium Iodide
488, 532, 561 617
200 tests
Propidium Iodide
488, 532, 561 617
500 tests
7-AAD
488, 532, 561 647
200 tests
7-AAD
488, 532, 561 647
500 tests
Cat. No.
13-0863-T100
13-0863-T500
13-0870-T100
13-0870-T500
13-0866-T100
13-0866-T500
13-0865-T100
13-0865-T500
13-6990-T200
13-6990-T500
13-6993-T200
13-6993-T500
Prezzo
50,00
180,00
50,00
180,00
50,00
180,00
50,00
180,00
30,00
60,00
18,00
50,00
Ghost Dyes™ Protocol
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Remove Ghost Dye vial from freezer and allow to equilibrate to room temperature.
Quick spin Ghost Dye vial before opening.
Prepare azide-free and protein/serum-free PBS for labeling procedure.
Wash cells twice in azide-free and protein/serum-free PBS.
Resuspend cells to a concentration of 1-10 x 106/mL in azide-free and protein/serum-free
PBS.
Add 1 uL of Ghost Dye solution for each 1 mL of cell suspension and vortex immediately.
NOTE: Ghost Dyes are formulated in DMSO, pipet carefully and slowly.
Incubate for 30 minutes at 2-8°C protected from light.
Wash cells 1-2 times with flow staining buffer containing serum or BSA. Washing with a
protein containing buffer allows removal of any unreacted dye prior to staining with
fluorescent antibodies.
Cells can be stained, fixed and permeabilized according to user protocol.
BIOTIME di G. Niccolai e C. sas
Via Salicotto, 143 – 53100 Siena
telefono 345 5180700
telefax 0577 221446
email [email protected]
website www.biotimenet.com
Codice Fiscale 00381250521
Partita IVA IT00381250521
Coordinate bancarie:
Banca Monte dei Paschi di Siena
IBAN: IT 54 W 01030 14200 0000 10648623