Pisa 12 Dicembre 2015 Prof. Daniela Proverbio Aimvet
¡  Associazione italiana
medicina trasfusionale
veterinaria
¡  11 Settembre 2012
¡  Università Milano, Pisa
Perugia, Bologna, Teramo,
Zooprofilattico di Padova
¡  Promuovere la crescita e la
conoscenza della medicina
trasfusionale
¡  Ottobre 2015 affiliata AIVPA
Formazione
DIPARTIMENTO:di:SCIENZE:MEDICHE:VETERINARIE
ALMA$MATER$STUDIORUM$!"UNIVERSITÀ"DI"BOLOGNA
Con$la$collaborazione$dell’Associazione$Italiana$Medicina$Emotrasfusionale$Veterinaria
MEDICINA:TRASFUSIONALE:VETERINARIA
Mercoledì:29:maggio:2013"!"ore$9:30
Aula$Messieri
Chairman:#Prof.#Paolo#FAMIGLI#BERGAMINI
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Prof.#Daniela#PROVERBIO*##
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Prof.#Daniela#PROVERBIO*##
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Presentazione#AIMVET
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Dr#Eva#SPADA*#
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Impiego#del#sangue#intero#e#del#plasma
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Emazie#concentrate,#valori#soglia
e#indicatori#clinici
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Pausa
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Prof.#Urs#GIGER**##
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Reazioni#trasfusionali#e#compa2bilità#ema2ca
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Pausa
Prof.#Urs#GIGER**##
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Applicazioni#cliniche#in#medicina#trasfusionale:#
anemia#emoli2ca#immunomediata#(IMHA)
e#altri$casi#clinici
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¡ Seminari
¡ Corsi di
perfezionamento
¡ Stage formativi
*"Dipar>mento$di$Scienze$Veterinarie$per$la$Salute,$la$Produzione$animale$e$la$Sicurezza$Alimentare,$Università$degli$Studi$di$Milano
**"Dipl.$ACVIM$e$ECVIM$(Internal$Medicine),$ECVCP$(Clinical$Pathology),$University$of$Pennsylvania
Il#seminario#è#gratuito
e#aperto#a#tu1#gli#interessa2
Per#informazioni
[email protected]
Medicina Trasfusionale Cane 1 Pisa 12 Dicembre 2015 Prof. Daniela Proverbio Ricerca
scientifica
Vet Res Commun (2009) 33 (Suppl 1):S201–S203
DOI 10.1007/s11259-009-9275-7
Veterinary Clinical Pathology ISSN 0275-6382
ABSTRACT
ORIGINAL RESEARCH
Assessment of blood types of Ragdoll cats for transfusion
purposes
Assessment of a gel column technique for feline
blood typing
Daniela Proverbio, Eva Spada, Roberta Perego, Alessandra Della Pepa, Giada Bagnagatti De Giorgi,
Luciana Baggiani
D. Proverbio & E. Spada & L. Baggiani & R. Perego
Department of Health, Animal Science and Food Safety, Unit of Veterinary Transfusion Medicine, University of Milan, Milan, Italy
Key Words
AB blood system, agglutination on gel, blood
donor, feline, pure bred cats
Correspondence
Eva Spada, via Celoria, 10, Milan 20133, Italy
E-mail: [email protected]
Published online: 4 July 2009
# Springer Science + Business Media B.V. 2009
DOI:10.1111/vcp.12048
Keywords Agglutination . Blood groups . Cat . Card . Column gel
Introduction
Blood typing is an essential tool in clinical feline practice to prevent serious transfusion and
neonatal isoerythrolysis reactions. An AB blood group system has been recognized in cats. As in
most species, blood type results from genetically-determined glycolipids and glycoproteins on
the surface of red blood cells (RBCs). Two gangliosides, N-acetyl - neuraminic acid (NeuAc)
and N-glycyl - neuraminic acid (NeuGc), are the most important to determinate feline blood
groups A and B. Type B cats express only NeuAc whereas type A cats express only NeuGc, type
AB cats express equal amounts of disialogangliosides containing NeuAc or NeuGc (Andrews et
al. 1992) The principle of a serologic feline blood-typing reaction is macroscopic agglutination
to allow detection of the different RBC surface antigens. The purpose of this study was to
evaluate the performance of a currently available column gel agglutination technique (Duguit
and Bromilow 1993) for feline blood group typing (Knottenbelt 2002; Stieger et al. 2005).
Background: Transfusion of compatible blood types ensures the vitality of
transfused erythrocytes and avoids transfusion reactions. Cats with types
A, B, and AB blood should receive transfusions of the same blood type. In a
feline blood donor program, it is therefore essential to have blood donors of
all blood types available.
Objectives: The objectives of this study were the identification of the 3
feline blood types in Ragdoll cats, the comparison of their frequencies with
those of Domestic Shorthair (DSH) cats, and the determination of whether
Ragdolls are suitable donors in a feline blood donor program.
Methods: The blood type was determined by gel column agglutination
from Ragdoll cats. The relationships between phenotypic traits, the origin
of the cats, and the different blood types were examined. The frequencies
for potential transfusion reactions and the risk for neonatal isoerythrolysis
(NI) were estimated.
Results: Of 61 typed Ragdolls, 77.1% had type A, 4.9% type B, and 18%
type AB blood. The frequency of blood type A in Ragdolls was lower than in
DSH cats (P = .02), while the frequency of blood type AB in Ragdolls was
higher than in DSH cats (P = .0002). No relationship was found between
blood type and origin of the cat or phenotypic traits. The estimated frequencies of major and minor transfusion reactions following an unmatched
transfusion between Ragdolls (donors and recipients), Ragdoll donors and
DSH recipients, and DSH donors and Ragdoll recipients were 4.7%, 6.7%,
4.6%, and 18.5%, 20.8%, 7.6%, respectively. The frequency of kittens at
risk for NI was 5%.
Conclusion: The presence of all 3 feline blood types and a relatively high
incidence of AB type cats make Ragdolls an ideal donor breed to include in
feline blood transfusion programs.
Introduction
The feline AB blood system consists of the 3 blood
types A, B, and AB. They are based on the presence
of different neuraminic acid residues on the surface
of RBCs. Type A cats have mainly N-glycolylneuraminic acid (NeuGc) and a small amount of
N-acetylneuraminic acid (NeuAc). Type B cats have
only NeuAc, while type AB cats have both NeuAc
and NeuGc present on the RBC surface in similar
quantities.1,2 All cats have naturally occurring alloantibodies against the other blood types, with the
exception of type AB cats because of the presence
Materials and methods
Venous blood was collected from 60 healthy and non-anaemic cats in tubes containing
EDTA. Each sample was blood typed in parallel using both a commercial column gel
agglutination technique for feline blood group typing (ID Gel-Test Micro Typing System,
Diamed) (Ginger et al. 2005; Stieger et al. 2005; Spada et al. 2007) and an agglutination
assay card method (RapidVet-H feline, Agrolabo) (Knottenbelt 2002; Stieger et al. 2005). If
there were discrepancies between the results obtained from the two methods, blood type
was confirmed with a back-typing hemoagglutination test (Griot-Wenk and Ginger 1995).
The column gel agglutination test (ID Gel-Test Micro Typing System, Diamed) consisted of
Vet Clin Pathol 0/0 (2013) 1–6 ©2013 American Society for Veterinary Clinical Pathology
of both antigens (NeuAc and NeuGc) on the RBC
surface.3
Blood typing and cross-matching in domestic cats
are vitally important as blood compatibility prevents
hemolytic transfusion reactions, such as major reactions between blood type B cats that receive type A or
AB blood. In addition, the vitality and life span of
transfused RBCs can be seriously affected by minor
reactions between blood type A cats that receive type B
or AB blood, and blood type AB cats that receive blood
type B.3,4 Hemolytic anti-B antibody titers have been
shown to fluctuate widely throughout the year in
some type A cats, which might explain why some type
1
D. Proverbio (*) : E. Spada : L. Baggiani : R. Perego
Dipartimento di Scienze Cliniche Veterinarie, Università degli Studi di Milano, via Celoria 10, 20133
Milano, Italy
e-mail: [email protected]
Associazione Italiana
Medicina
Emotrasfusionale
Veterinaria (AIMVET)
ottobre 2015 affiliata AIVPA
Medicina Trasfusionale Cane 2