Eco-physiological adaptation of model polychaete species to climate change and laboratory rearing protocols Alexia Massa Gallucci Scientific Coordinator: Maria Cristina Gambi Laboratory of Functional and Evolutionary Ecology, Stazione Zoologica Anton Dohrn, Napoli research team at Villa Dohrn Ischia CO2 vents and acidified areas off Ischia Castello Aragonese, Ischia North side Seawater pH 7.0 South side Extreme Low Low Ambient pH gradient 8.1 Model Polychaete Species Amphiglena mediterranea (Leydig,1851) Sabellidae Size: 4-5 mm Ecology: littoral vegetated rocky reef, and seagrass meadows Trophic habits: filter feeder Reproductive biology: iteroparous, brooder inside the tube Geographic distribution: Mediterranean Sea and North Atlantic Ocean Syllis prolifera (Krohn,1852) Syllidae Size: 5-25 mm Ecology: littoral vegetated rocky reef, and seagrass meadows Trophic habit: mesograzer Reproductive biology: iteroparous, sexual stolons Geographic distribution: Mediterranean Sea and North Atlantic Ocean Project objective: Rearing Protocols • Set-up of standardized rearing protocols to obtain a multi-cohortpopulation of model species Project objective: Eco-physiological adaptation to Climate Change Study biological and eco-physiological adaptation to climate change of model species: • In situ reciprocal transplant experiments and measure of metabolic rates and biomarkers • Test of pH and temperature variation on labreared population: biometric data, tissue regeneration and reproductive output