Liceo classico “Gonzaga” Milano UTILIZZARE SPAZIO PER INSERIRE FOTO/IMMAGIN E DI RIFERIMENTO LEZIONE Science course From Prokaryotes to Eukaryotes Teacher Maria Elena Scortecci Microscope eye pieces revolver stage focus knob diafragm and condenser lens light source 2 Scientific classification Prokaryotes (bacteria and archaea) • unicellular (cell size 1-10 µm) • no nucleus (karyon) and organelles • special cell wall • single circular cromosome + plasmids • aerobic or anaerobic • reproduction by fission 3 Scientific classification Eukaryotes (amoeboroa, fungi, animalia, plantae) • uni-multicellular (cell size 10100 µm) • several pairs of chromosomes in a nucleus • many organelles • only aerobic • sexual reproduction 4 4 How to observe cells blotting paper dye (i.e. methylene blue) pipettes water coverslips microscope slides paddle 5 Eukaryotes animalia (human buccal mucosa) • round cells • small blue nucleus • cytoplasmic membrane • cytoplasm • organelles 6 6 Prokaryotes bacteria (lactobacillus) from yoghurt • very small cells: 2 µm long • form filaments • metabolize lactose • produce lactic acid • no organelles 7 7 Eukaryotes fungi (saccharomyces cerevisiae) from brewer yeast • oval cells • nucleuses • dividing cells by budding • use glucose to produce energy, breathing oxigen 8 8 Eukaryotes plantae (pelargonium) • prismatic cells in mutual contact • stiff • typical organelles: chloroplasts, chromoplasts, amyloplasts • no dye, because they are naturally stained cells • photosynthetic cells: CO2 + H2O glucose + oxygen 9 9 Eukaryotes plantae cyclamen leaves, with stomata and puzzle shape 10 10 Eukaryotes plantae onion cell 11 11 Eukaryotes plantae stomata cells in Setcrasea 12 12 Eukaryotes plantae tracheides in fennel 13 13 Copyright 2012 © eni S.p.A. 14 14