Curriculum Vitae of dr. Alberto Vitali Born: 16th January 1965 in Avezzano (AQ) Address: via Pietro Maffi, 161 – 00168 Roma, Italy Phone: +39‐06‐86908654 Education and work experiences From 21 December 2001 to present: research scientist at the Institute of Chemistry of Molecular Recognition, CNR UoS Roma (formerly Centre for Chemistry of Receptors and Biologically Active Molecules). From 17 November 1997 to 16 May 1999: employed at RADIM SpA, Pomezia (production area). From 15 May 1992 to present: registered in the National Order of Biologists, No 038095, Section A. February 1990 ‐ Degree in Biology at the University "La Sapienza" of Rome. Mark: 110/110. Experimental thesis title: "Secondary metabolites from plant cell cultures of Maclura pomifera ". Supervisors Prof. Giovanni Battista Marini Bettolo and Prof. Bruno Botta. Scientific Activity Dr. Vitali has carried out his experimental thesis at the Institute of Chemistry of Molecular Recognition (ICRM), CNR ‐UoS Roma ‐ (formerly Center for Chemistry of Receptors and Biologically Active Molecules) dealing with the study of natural compounds with particular regard to flavonoids, both from a structural and biosynthetic point of view. Together with this activity he began the study of enzymes of plant origin and in particular of peroxidases, key enzymes in some biosynthetic steps of flavonoids. Subsequently it was interested in the application of such enzymes (both in crude and purified forms) as biocatalysts in studies of bioconversion of synthetic and natural substrates in order to obtain high added value molecules. For a period of 18 months he was employed at RADIM SpA (Pomezia), a private company producer of diagnostic kits, specializing in the purification of monoclonal antibodies and in their stabilization in order to optimize the performance of the kits. In 2000 he reached again the Institute of Chemistry of Molecular Recognition where, due to organizational changes of the Institute, he began the study of biologically active peptides and proteins, acquiring skills and knowledge in mass spectrometry and spectroscopy of proteins and their related techniques. In December 2001 he was appointed Researcher at ICRM. Presently, he is interested in the study of biologically active peptides in particular as modulators of cellular functions and as antimicrobial and antiviral agents. In this view he is developing peptide sequences able to interact with protein domains such as SH3 crucial for various cellular functions in order to understand the physiological roles of such peptides and also to develop new potential therapeutics. In parallel he is also interested in the peptidomic study of biological fluids, saliva firstly, and in proteomic studies of diverse pathologies approached mainly by 2DE and mass spectrometry related techniques. His scientific activity is represented by various peer‐reviewed publications and communications at national and international congresses. Teaching activity From 2002 to present, beside the research activity, dr. Alberto Vitali has been appointed as contract professor for the Catholic University of Rome in Organic Chemistry and Biochemistry. He exploits the didactic activity also as supervisor for thesis students of scientific faculties (Biological Sciences, Pharmaceutical Technology, Medical Biotechnology, Pharmacy) of Sapienza University in Rome and for PhD students of Catholic University of Rome. He had to lead and organize one post‐doc for a period of one year. Technical skills Purification and characterization of plant metabolites Purification and characterization of proteins and peptides Plant and mammals cell cultures (maintaining, cell viability tests, motility tests) Fluorescence microscopy Chromatography (HPLC, FPLC) and electrophoresis (2DE, blotting) related techniques Mass spectrometry (ESI‐ IT‐ LC‐MS, MALDI‐ToF MS) and related techniques FTIR, Circular Dichroism, Fluorescence, UV spectroscopy techniques Project participation -2011‐2013‐Research project funded by CARIPLO Foundation "Innovative materials for medical devices based on biofunctionalized surfaces and antifouling properties", Coordinator Prof. F. Cappitelli (University of Milan). ‐2008‐2009‐Metadistretti Regione Lombardia – in collaboration with LOFARMA S.p.A. ‐Research Project co‐financed by the Catholic University of Rome, 2009, "Nucleofphosmin‐DNA interaction by SPR." Coordinator Prof. A. Arcovito. ‐Research Project co‐financed by the Catholic University of Rome, 2006, "Study of the peptides in biological fluids with integrated analytical strategies (proteomics of body fluids). Coordinator Prof. M. Castagnola. ‐Research Project co‐financed by the Catholic University of Rome, 2005, "Study of the peptides by capillary electrophoresis and HPLC‐MS." Coordinator Prof. M. Castagnola. ‐2004‐2006 Antarctic Project (PNRA ID No., COCCAX4), as participant in the O.U. of Prof. B. Giardina. ‐F.I.R.B. 2003, Enzymes and organo‐metallic catalysts for new biotransformations and optimization of production processes environmentally friendly. Protocol No. RBNE034XSW_004, as participant in the O.U. of Prof. B. Giardina. ‐P.R.I.N. 2003, Protocol No. 2003050353_002, as participant in the O.U. of Prof. M. Castagnola (Catholic University of Rome). ‐From 1st November 1999 to 1st November 2001 at grant from INDENA SpA in the study of “Enzymatic modification of flavonolignans (silybin) useful for therapeutic use” ‐European Project 1997, AIR2‐CT‐94‐1343 n. 7090241, AZTEC: “Innovative Technologies to Produce Environmentally Sound Natural Pesticides from Neem tree (Azadirachta spp.)”. ‐From 13 November 1995 to 12 September 1996: scholarship from Tecnofarmaci SCpA within the project "isocordoin and its derivatives against skin aging." ‐From 9 January 1993 to 31 August 1994 fellowship within the project "Methodologies for the use of immobilized and in solution enzymes for bioconversion, biosensors and bioreactors" Call n. 201.12.63/3 in Progetti Finalizzati “Biotechnology and Bioinstrumentation”. Schools, courses and other experiences December 2005, Center of mass spectrometry LSMBO CNRS headed by professor Alain VAN DORSSELAER, at the Ecole des Polymer Materiaux Chimie de Strasbourg . ‐ School of 2‐D electrophoresis c/o CNR‐ISPA BioIndustry Park, 5 ‐ 7 February 2003. ‐ IV Course of Integrated Mass Spectrometry in the study of genome and proteome. Vitorchiano (VT), 9‐12 December 2002. ‐ National School of Protein Science: "Methods of separation and analysis", 13‐17 September 1993, Urbino. ‐ "Intensive Course on the purification, characterization and production of proteins in Pharmacological Research", 8‐10 September 1992, Rome. ‐ School of Computational Chemistry: "Approaches to the Study of Structure‐Property Relationship," 20 to 25 July 1992 Pontignano (Siena). ‐ "Biotechnology of Plant Cells", Milan, October 18, 1991. ‐ V National School of Protein Science, "NMR Spectroscopy of protein systems," 23 to 28 June 1991 Pontignano (Siena). Most relevant publications 1. “Altered expression level of Escherichia coli proteins in response to treatment with the antifouling agent zosteric acid sodium salt”. Villa F, Remelli W, Forlani F, Vitali A, Cappitelli F. Environ. Microbiol.doi: 10.1111/j.1462‐2920.2011.02678.x. 2. “Biotechnological implications of the salivary proteome”. Review. Castagnola M, Cabras T, Vitali A, Sanna MT, Messana I. Trends Biotechnol. 2011; 29(8): 409‐418. 3. “Synthesis and molecular modelling studies of prenylated pyrazolines as MAO‐B inhibitors”. Fioravanti R, Bolasco A, Manna F, Rossi F, Orallo F, Yáñez M, Vitali A, Ortuso, F, Alcaro S. Bioorg Med Chem Lett. 2010; 20(22): 6479‐6482. 4. “The surprising composition of the salivary proteome of preterm human newborn”. Castagnola M, Inzitari R, Fanali C, Iavarone F, Vitali A, Desiderio C, Vento G, Tirone C, Romagnoli C, Cabras T, Manconi B, Sanna MT, Boi R, Pisano E, Olianas A, Pellegrini M, Nemolato S, Heizmann CW, Faa G, Messana I. Mol Cell Proteomics. 2011; 10(1): M110.003467. 5. “Antimicrobial activity of human hepcidin 20 and 25 against clinically relevant bacterial strains: effect of copper and acidic pH”. Maisetta G, Petruzzelli R, Brancatisano FL, Esin S, Vitali A, Campa M, Batoni G. Peptides. 2010; 31: 1995‐2002. 6. “Structural characterization of a new statherin from pig parotid granules”.Manconi B, Fanali C, Cabras T, Inzitari R, Patamia M, Scarano E, Fiorita A, Vitali A, Castagnola M, Messana I, Sanna MT. J Pept Sci. 2010 Jun;16(6):269‐75. 7. “Expression, purification, phosphorylation and characterization of recombinant human statherin”. Sanna M.T. Manconi. B, Vitali A., Fanali C., Fiorita A., Inzitari R., Castagnola M., Messana I. Protein Expression and Purification, 2010; 69: 219‐225. 8. “‐2 strand of salivary S cystatins: a ‘chemeleon’ sequence”. Vitali, A. Alinovi, C.C., De Rosa M.C., Petruzzelli R., Biochem. and Biophys. Res. Comm., 2009; 387:47‐51. 9. “A proteomic approach to characterizing ciglitazone‐induced cancer cell differentiation in Hep‐G2 cell line”. Bottoni P, Giardina B, Vitali A, Boninsegna A, Scatena R. Biochim. Biophys Acta. 2009 ; 1794: 615‐626. 10. “Localisation of Bgl2p upon antifungal drug treatment in Candida albicans”, Angiolella L, Vitali A, Stringaro A., Mignogna G., Maras B., Bonito M., Colone M., Palamara A.T., Cassone A. Int J Antimicrob Agents. 2009, 33(2):143‐148. 11. “Antifungal‐protein production in maize (Zea mays) suspension cultures Perri F, Della Penna S., Rufini F., Patamia M., Bonito M., Angiolella L., Vitali A.. Biotechnol. Appl Biochem. 2009; 52(Pt 4): 273‐281. 12. “Trafficking and post‐secretory events responsible for the formation of secreted human salivary peptides. A proteomic approach”. Messana I, Cabras T, Pisano E, Sanna MT, Olianas A, Manconi B, Pellegrini M, Paludetti G, Scarano E, Fiorita A, Agostino S, Contucci AM, Calò L, Picciotti PM, Manni A, Bennick A, Vitali A, Fanali C, Inzitari R, Castagnola. M Mol Cell Proteomics. 2008 ;7, :911‐926. 13. “Different structural behaviors evidenced in thaumatin‐like proteins: a spectroscopic study” Perri F, Romitelli F, Rufini F, Secundo F, Di Stasio E, Giardina B, Vitali A. Protein J. 2008; 27(1):13‐20. 14. “Structural and functional characterization of the porcine proline–rich antifungal peptide SP‐B isolated from salivary gland granules T. Cabras, R. Longhi, F. Secundo, G. Nocca, S. Conti, L. Polonelli, C. Fanali, R. Inzitari, R. Petruzzelli, I. Messana, M. Castagnola, A. Vitali. Journal of Peptide Science. 2008; 14:251‐260. 15. “Purification and characterization of an antifungal thaumatin‐like protein from Cassia didymobotrya cell culture”. Vitali A., Pacini L., Bordi E., De Mori P., Pucillo L., Maras B., Botta B., Brancaccio A., Giardina B. Plant Physiol Biochem. 2006, 44:604‐610. 16. “Detection in human saliva of different statherin and PB fragments and derivatives”. Inzitari R., Cabras T., Rossetti D.V., Fanali C., Vitali A., Pellegrini M., Paludetti G., Manni A., Giardina B., Messana I., Castagnola M. Proteomics. 2006; 6: 6370‐6379. 17. “Chalcone inhibition of anthracycline secondary alcohol metabolite formation in rabbit and human heart cytosol” Silvestrini A., Meucci E., Vitali A., Giardina B., Mordente A., Chem Res Toxicol., 2006; 19:1518‐1524. 18. “Fragments of human salivary statherin and PB peptide underlying a furin‐like pro‐protein convertase action in the pre‐secretory salivary fragmentation pathway”, Inzitari R., Rossetti D.V., Cabras T., Olmi C., Fanali C., Vitali A., Pellegrini M., Messana I., Castagnola M., FEBS J., 2005, 272, 156‐156. 19. “Two proline‐rich peptides from pig (Sus Scrofa) salivary glands generated by pre‐secretory pathway underlying the action of a proteinase cleaving Pro‐Ala bonds”. Patamia M., Messana I., Petruzzelli R., Vitali A., Inzitari R., Cabras T., Fanali C., Scarano E., Cantucci A., Galtieri A., Castagnola M., Peptides, 2005; 26, 1550‐1559. 20. “Prenylated flavonoids: pharmacology and biotechnology”. Botta B, Vitali A, Menendez P, Misiti D, Delle Monache G. Curr Med Chem. 2005;12(6):717‐739. 21. “Statherin levels in saliva of patients with precancerous and cancerous lesions of the oral cavity: a preliminary report” AM Contucci, R Inzitari, S Agostino,Vitali A., A Fiorita, T Cabras, E Scarano, I Messana. Oral Diseases 2004; 10, 1–5. 22. “Chalcone dimethylallyltransferase from Morus nigra cell cultures. Substrate specificity studies”. A. Vitali, B. Giardina, G. Delle Monache, F. Rocca, A. Silvestrini , A. Tafi, B. Botta, FEBS Lett. 2004; 557, 33‐38. 23. “Determination of the post‐translational modifications of salivary acidic proline‐rich proteins”. Castagnola M, Cabras T, Inzitari R, Zuppi C, Rossetti DV, Petruzzelli R, Vitali A., Loy F, Conti G, Fadda MB. Eur J Morphol. 2003; 41:93‐98. 24. “Human salivary percentages of cystatin S, S1 and S2”, Lupi A, Gambarini G, Testarelli L, Bolognini G, Piras V, Messana I, Denotti G, Cordaro M, Vitali A, Castagnola M, J. of Dental Res., 2003, 82, Sp. Iss. C, 559‐559. 25. “Identification of the human salivary cystatin complex by the coupling of high‐performance liquid chromatography and ion‐trap mass spectrometry” A. Lupi, I. Messana, G. Denotti, M.E. Schininà, G. Gambarini, M.B. Fadda, A. Vitali, T. Cabras, V. Piras, M. Patamia , M. Cordaro, B. Giardina, M. Castagnola. Proteomics. 2003,3: 461‐467. 26. “Affinity capillary electrophoresis study of the linkage existing between proton and zinc ion binding to bacitracin A(1)”. Castagnola M, Rossetti DV, Inzitari R, Vitali A, Lupi A, Zuppi C, Cabras T, Fadda MB, Petruzzelli R, Giardina B, Messana I Electrophoresis. 2003, 24:801‐807 27. “Capillary electrophoretic study of the binding of zinc(II) ion to bacitracin A1 in water‐2,2,2‐
trifluoroethanol”. Castagnola M, Rossetti DV, Inzitari R, Vitali A, Lupi A, Zuppi C, Cabras T, Fadda MB, Podda I, Petruzzelli R, Giardina B, Messana I. Electrophoresis. 2003, 24, 1612‐1619. 28. “Characterization of dendrimer properties by capillary electrophoresis and their use as pseudostationary phases”. M.Castagnola, C. Zuppi, D.V. Rossetti, F.Vincenzoni, A. Lupi, A. Vitali, E. Meucci, I. Messana. Electrophoresis; 2002, 23, 1769‐1778. 29. “Capillary electrophoresis detection of a complex sensitive to electric field”, Messana I, Bassi FA, Rossetti DV, Misiti F, Vincenzoni F, Vitali A, Zuppi C, Giardina B, Castagnola M, J. of Sep. Science. 2001; 24, 717‐722. 30. “Aryltetraline Lignans: Chemistry, Pharmacology and Biotransformations”. B. Botta, G. Delle Monache, D. Misiti, A. Vitali, G. Zappia. Current in Med. Chemistry , (2001), 8, 1373‐1392. Patents 1) IT RM2011A000035, PCT PCT/IB2012/050415 2) IT RM2011A000036, PCT PCT/IB2012/050419 3) IT RM2011A000037, PCT PCT/IB2012/050421 Congress partecipation and abstracts 1. Pirolli D., Vitali A., Radicioni G., Giardina B., Castagnola M., De Rosa. “Molecular interactions of a proline‐rich peptide with the SH3 domain of tyrosine‐protein kinase: a computational approach”. XIII Workshop on bioactive peptides, Napoli 7‐10 giugno 2012. 2. Vitali A., Mazzoni M., Radicioni G, Molinari A., Stringaro A., Messana I., Castagnola M., , Palmerini C.A. “Antagonistic effects of a salivary proline‐rich peptide on the cytosolic Ca2+ concentration induced by progesterone in an oral squamous cancer cells line”. XIII Workshop on bioactive peptides, Napoli 7‐10 giugno 2012 3. G. Maisetta, A. Vitali, R. Petruzzelli, F. L. Brancatisano, S. Esin, A. Stringaro, M. Colone, C. Luzi, A. Bozzi, M. Campa, G. Batoni. “Effect of pH on the interaction of the human antimicrobial peptides hepcidin 20 and 25 with Escherichia coli ATCC25922 and model membranes”. New Antimicrobials Project, 2nd Workshop, 25‐26 maggio 2012, Università di Trieste. 4. A Vitali, G. Maisetta, F. L. Brancatisano, S. Esin, R. Petruzzelli, M. Campa, G. Batoni. “Antimicrobial properties of human hepcidins 20 and 25”. XII Worshop on bioactive peptides, Napoli 4/7 Giugno 2010. 5. A. Vitali, C. Fanali, R. Longhi, S. Conti, L. Polonellli, R. Inzitari, I. Messana, T. Cabras, B. Manconi, M. Castagnola. Antimicrobial proline‐rich peptides from secretory parotid granules of pig (Sus scrofa)”. XI Worshop on bioactive peptides, Napoli 24/27 Maggio 2008. 6. C. Fanali, B. Manconi, E. Scarano, M.T. Sanna, R. Inzitari, T. Cabras, M. Patamia, A. Vitali, I. Messana, M. Castagnola The primary structure of pig salivary statherin 8th European Symposium on Saliva Egmond aan Zee, The Netherlands, May 14‐17, 2008. Abstract Pag. 75 7. “A 2733 Da proline rich antifungal peptide from pig salivary secretory granules. Biological activity and structural studies”, A. Vitali, C. Fanali, F. Perri, V. Fara, R. Longhi, M. Castagnola, I. Messana, S. Conti. 52° Congresso Nazionale SIB – 2007, Riccione 8. “Oxidative metabolism alteration induced by Proline‐rich peptides in PJ15 oral cancer cell line”. A. Vitali, R. Longhi, G: Nocca, C. Fanali, E. Scarano, R. Inzitari, T. Cabras, A. Finocchiaro, B. Gavazzi, 52° Congresso Nazionale SIB – 2007, Riccione. 9. “Cystatin structure: a case of non hierarchical protein folding”, A. Vitali, C. De Rosa, C. Carelli Alinovi, R. Petruzzelli, 52° Congresso Nazionale SIB – 2007, Riccione. 10. “Characterization of proline‐rich peptides from pig parotid glands” C. Fanali, R. Inzitari, D. V. Rossetti, T. Cabras, R. Petruzzelli, M. Patamia, A. Vitali, E. Scarano, S. Agostino, I. Messana, M. Castagnola. Stensen III°; 3rd Intern. Symposium on Salivary Glands in Honor of Niels Stensen. Okazaki (Japan) – 20‐24 october 2006. 11. “Enzymatic halogenation of chalcones”, A. Vitali, A. Silvestrini, B. Giardina BIOCATALYSIS 2006, Hamburg. 12. “Comparison of thaumatin‐like protein’s structures by Intrinsic Fluorescence spectroscopy”, F. Perri, F. Romitelli, E. Di Stasio, F. Rufini, B. Giardina , A. Vitali PROTEINE 2006, Novara. 13. “Modulation of differentiation state of human hepatocarcinoma cell lines analyzied by a preliminary proteomic approach”, P. Bottoni, B. Giardina, G.E. Martorana, A. Vitali, G. Botta, P. Pastore, C. Zuppi, R. Scatena The Italian Proteomics Association, CNR Area della ricerca di Pisa, 1st annual congress, “2‐4 Luglio 2006. 14. “Fragments of human salivary statherin and PB peptide underlying a furin‐like pro‐protein convertase action in the pre‐secretory salivary fragmentation pathway” R. Inzitari, D.V. Rossetti, T. Cabras, C. Olmi, C. Fanali, A. Vitali, M. Pellegrini, I. Messana, M. Castagnola. 30th FEBS Congress and 9th IUBMB Conference, Budapest 2‐7 july 2005. FEBS J. 272 (Suppl. 1), (2005); pg 156 (B2‐026P). 15. “Caratterizzazione biochimica e localizzazione parietale di una beta1‐3 glucanasi in Candida albicans” L. Angiolella, A. Vitali, M.A. Bonito, B. Maras, A. Stringaro, M. Colone, A. Cassone, A.T. Palamara, 33° Congresso Nazionale SIM, Napoli 16‐19 Ottobre 2005. 16. “Different human salivary small basic proline‐rich peptides deriving from multiple repeats present on PRB1‐PRB2 loci” I. Messana, D.V. Rossetti, R. Inzitari, T. Cabras, C. Olmi, C. Fanali, A. Vitali, M. Castagnola. Massa 2005, Congresso annuale della Div. Spettrom. di Massa della SCI, Roma 28 giugno‐1° luglio 2005. Book of abstract pg. 118‐119. 17. “Different phosphorylations of salivary histatin 1 determined by HPLC‐ESI‐MS”. G. Onnis, T. Cabras, C. Olmi, A. Vitali, E. Pisano, M. Castagnola, I. Messana XVI Meeting of the Sardinian Group of the Italian Soc. of Biochem. And Molecular Biology, Sassari, 24 june 2005. Italian J. Biochem. 54, (Suppl. 1) 2005, pg. 15 18. “Determination of anomalous physico‐chemical properties of a salivary basic proline‐rich peptide from pig (Sus scrofa domesticus) by capillary electrophoresis”.T. Cabras, E. Pisano, G. Onnis, A. Mastinu, R. Inzitari, A. Vitali, C. Fanali, M. Castagnola, I. Messana 14th International symposium on capillary electroseparation techniques. Roma, 12‐15 settembre 2004. 19. “Comparative study of mammal salivary proteome: the identification of two salivary proline‐rich peptides from sus scrofa, fragments deriving from a common DNA sequence” M. Patamia, I. Messana, R. Petruzzelli, A. Vitali, R. Inzitari, T. Cabras, D.V. Rossetti, B. Giardina, M. Castagnola. I° Congress of Italian Proteome Society (IPSO), Verona, may 27‐29 2004. Book of abstracts, pag. 33. 20. “Determination of the post‐translational modifications of salivary acidic proline‐rich proteins.” M. Castagnola, T. Cabras, R. Inzitari, C. Zuppi, D.V. Rossetti, R. Petruzzelli, A. Vitali, F. Loy, F. Testa‐Riva, M.B. Fadda. 5th Internat. Malpighi Symposium – Rome, September 11‐13, 2003 Italian J. Anat. And Embriol.108 (Suppl. 1) (2003) 49. 21. “Thermodynamics of metal ion binding to bacitracin A1 studied by capillary electrophoresis.” M. Castagnola, D.V. Rossetti, R. Inzitari, A. Vitali, C. Zuppi, T. Cabras, M.B. Fadda, I. Podda, I. Messana. 5th european Symposium of the Protein Society, Firenze, 29 march ‐ 2 april 2003; Protein Science 12 (Suppl. 1) 51 (2003). 22. “Analysis of the salivary cystatin complex by the coupling of HPLC‐ion trap mass spectrometry.” I. Podda, M. Castagnola, I. Messana, G. Denotti, A. Lupi, A. Vitali, V. Piras, T. Cabras, M.B. Fadda. Atti XV° riunione SIB‐Sardegna, Sassari, 21 giugno 2002. 23. “Human salivary percentages of cystatin S, S1 and S2.”A. Lupi, G. Gambarini, L. Testarelli, G. Bolognini, V. Piras, I. Messana, G. Denotti, M. Cordaro, A. Vitali, M. Castagnola. 1° PEF meeting of the IADR; Cardiff, 25‐29 september 2002. 24. “Detection of Human Salivary peptides by HPLC‐ESI‐MS coupling” 80th General Session of IADR, 31th Annual Meeting of AADR, 26th Annual meeting of CADR, San Diego (CA, USA) 6‐9, marzo 2002, J. Dent. Res. 81 (Special issue A), A281 (2198). 25. “Different capillary electrophoresis options for the analysis of salivary proteins” 80th General Session of IADR, 31th Annual Meeting of AADR, San Diego (CA, USA) 6‐9 marzo 2002, J. Dent. Res. Special Issue A A‐281, (2199). 26. “The determination of chemico‐physical peptide properties by capillary electrophoresis”, Simposio Acta Biophysica Romana”, Roma, 18‐19 Aprile 2002. 27. “Aromatic prenyltransferase from plant cell cultures. Specificity and biotransformation studies”, A. Vitali, G. Delle Monache, F. Rocca, B. Botta, B. Giardina, p. 369 International Symposium on the Chemistry of Natural products 23rd IUPAC 2002, 28/7‐2/8 Firenze. 28. “Protein production, isolation and characterization in plant cell cultures of Cassia didymobotrya”, A. Vitali, B. Maras, M.E. Schininà, L. Pacini, B. Botta, B. Giardina, p. 399, International Symposium on the Chemistry of Natural products 23rd IUPAC 2002, 28/7‐2/8 , Firenze. 29. “Biotransformation of synthetic dibenzylbutanolides with purified peroxidase. Kinetic studies”. VI International Plant peroxidases Symposium, Murcia, Spagna, 3‐7 Luglio 2002. 30. “Proteomic of human saliva by mass spectrometry” A. Lupi, A. Vitali, M.E. Schininà, I. Messana, T. Cabras, M.B. Fadda, G. Denotti, V. Piras, B. Giardina, M. Castagnola. 46° Congr. Naz. SIB, Siena, 26‐
29 Settembre 2001. 31. “Analysis of several proteins of human saliva by micellar electrokinetic capillary chromatography”, I. Messana, M. Castagnola, C. Zuppi, T. Cabras, R. Murtas, D. Congiu, G. Gambarini, F. Misiti, D.V. Rossetti, A. Vitali, A. Lupi, M.B. Fadda37th IADR CED meeting, Rome, 5‐8 september 2001. 32. “Prenyl‐transferase from plant cell cultures of Morus nigra. Biotransformation studies ”, Future Trends in Phytochemistry", Rolduc, The Netherlands, Maggio 2000. 33. Convegno di Biotecnologie Vegetali, (Convegno S.B.I., Genova): “Biotrasformazioni di Calconi mediante colture cellulari di Morus nigra”. Pasqua G., Monacelli B., Botta B., Delle Monache G., Vitali A., 1999 34. "Plant cell cultures: Flavonoids production and biotransformation studies".Scuola di Fitochimica "Paolo Ceccarelli", Furore (Sa), 29 Giugno 1998: 35. "Biotrasformazioni di polifenoli mediante perossidasi vegetali purificate".IV Convegno Nazionale Giornate di Chimica delle Sostanze Naturali NAT4, Salerno, 24‐26 Settembre 1997. 36. "Produzione e localizzazione tissutale di antranoidi prenilati in Vismia guianensis D.C.", "Piante Officinali: attualità e prospettive scientifiche", Roma, 20‐21 Giugno 1997. 37. "Biotransformation of chalcones by purified peroxidase isolated from plant cell cultures of Cassia didymobotrya" (comunicazione orale), "Piante Officinali: attualità e prospettive scientifiche", Roma, 20‐21 Giugno 1997. 38. “Purification of a peroxidase from plant cell cultures of Cassia didymobotrya and biotransformation studies”."New Trends in Biotechnology '97 ‐ Science and Education", Capri, 26 ‐28 Maggio 1997. 39. “Biotransformation of dibenzylbutanolides by cell free extracts of Cassia didymobotrya”, B. Botta, G. Delle Monache, A. Vitali*, P. Ricciardi, M.C. De Rosa, D. Misiti, "Future Trends in Phytochemistry", Rolduc, The Netherlands, 12‐15 Maggio 1996. 40. “Biotrasformazioni di polifenoli mediante perossidasi di Cassia didymobotrya” B.Botta, G. Delle Monache, A. Vitali, P. Ricciardi, D. Misiti. “Biocatalisi e Sintesi Organica”, Acquafredda di Maratea, 3‐5 Maggio 1996, C20. 41. "Biotransformation studies by a Peroxidase Enzyme from plant cell cultures", B. Botta, M.C. De Rosa, R. Scurria, A. Vitali, G. Delle Monache. Honolulu (USA) Dicembre 1995: 1995 International Chemical Congress of Pacific Basin Society. 42. "The biotransformation of chalcones to aurones and auronols", A. Vitali, B. Botta, G. Delle Monache, M.C. De Rosa, R. Scurria, D. Misiti. BIOTRANS '95, University of Warwick, Coventry, UK. 5‐
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