Aumento della Risoluzione in funzione del Particle Size
mAU
175
150
125
100
75
50
25
0
0
1
2
4
0.25
6
5
3
0.5
0.75
7
1
1.25
1.8 um
1.5
1.75
2
min
mAU
175
1
150
4
2
125
100
3
75
6
5
7
3.5 um
1
2
3
4
5
6
7
4-Acetamidophenol
Caffeine
2-Acetamidophenol
Acetanilide
Acetylsalicylic Acid
Phenacetin
Salicylic Acid
50
25
0
0
0.25
0.5
0.75
1
1.25
1.5
1.75
2
min
mAU
175
1
150
125
4
2
100
6
5
3
75
7
5 um
50
25
0
0
0.25
0.5
0.75
1
1.25
1.5
1.75
2
min
LC Conditions
Column: SB-C18, 4.6 x 30 mm
Detector: 254 nm
Injector: 1 ul,
Mobile Phase: 1% Formic Acid
Acetonitrile
(82:18)
Flow: 2.0 ml/min
Piccola quantità
di particelle più
grandi
Particle Size (um)
4,0
3,8
3,6
3,4
3,2
3,0
2,8
2,6
2,4
2,2
2,0
1,8
1,6
1,4
1,2
1,0
0,8
0,6
0,4
0,2
0,0
Meno particelle
più piccole
Aumento della Risoluzione in funzione del Particle Size
5µm:
1.8µm:
Rs(4,5) = 3.63
Peak Width, 5s (9) = 0.103
+ 50% Resolution (Theory: + 67%)
+ 125% Efficiency (Theory: + 177%)
Rs(4,5) = 5.45
Peak Width 5s(9) = 0.075
Rs = +50%
0
1
2
3
4
5
6
7
min
Colonna SB-C18 RRHT 4.6 x150mm
Massima lunghezza
AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100
1 2
4.6 x 250 mm, 5 mm
1 mL/min
3
Rs(1,2) = 4.8
N=21848
0
1 2
5
3
10
15
20
Rs(1,2) = 3.5 12.71
0
5
N=6463
10
0
15
20
5
25
15
12 3
20
25
1100 HT + 1.8um RRHT :
30
min
30
min
4.6 x 30 mm, 1.8 mm
2 mL/min
4
10
min
4.6 x 30 mm, 1.8 mm
1 mL/min
Rs(1,2) = 3.1
2.09
N=6460
30
4.6 x 100 mm, 3.5 mm
1 mL/min
4 N=11691
1
5
10
.0 2
3N=6568
4.15 Rs(1,2) = 3.3
4N=6104
25
29.65
4N=22680
0.5
15
•
1
1.5
20
2
2.5
25
30
min
Reduction of analysis time from 30 min to 2
min
•
•
•
Speed: 14x faster than 4.6 x 250mm, 5um
Time saving: 92%
Resolution: -35%
Higher flow rate:
higher speed – same resolution –
higher sensitivity/precision
Sample:
Column:
2 ml/min – 90bar
1
Rs2,1 = 2.2
4
2
3
7 Analgesics
Zorbax RRHT SB-C18,
1.8 um, 4.6 x 30 mm
6
5
7
Rs7,6 = 2.2
1
2
3 ml/min – 133bar
4
3
6
5
7
4 ml/min – 181bar
1
2 2,1 = 42.1
Rs
3
0.0
0.1
0.2
5
0.3
6
Rs7 7,6 = 2.2
0.4
0.5
Time (min)
0.6
0.7
Ultra-fast analysis in 26 sec
4 peak width ~ 0.6 sec
< 50 sec cycle time
0.8
0.9
1.0
2x faster than 2ml/min
without loss in
resolution!
7 analgesics separated in 21 sec
for peaks 1,2,3
Total time Injection-to-Injection
Speed
Rapid Resolution HT Columns – Up to 20X Faster
Up to 20x faster than conventional HPLC
• With same or better performance (resolution, precision, sensitivity, carry over)
• Compliant with strictest regulatory performance requirements
SB-C18
4.6 x 150mm, 5µm
1.20ml/min, 40°C
Analysis Time = 11min
HPLC, 40°C
0
2
4
6
8
10
min
RRHT SB-C18
2.1mm x 50mm 1.8µm
1.00ml/min, 40°C
Analysis Time= 1.1min
RRLC, 40°C
10x faster
0
RRHT SB-C18
2.1mm x 50mm 1.8µm
2.40ml/min, 95°C
Analysis Time: 0.4min
0.2
0.4
0.6
0.8
1
min
RRLC, 95°C
27x faster
0
0.2
0.4 min
0.6
>20X faster
NON SOLO VELOCITA’ MA ANCHE EFFICIENZA
5µm:
1.8µm:
Rs(4,5) = 3.63
Peak Width, 5s (9) = 0.103
+ 50% Resolution (Theory: + 67%)
+ 125% Efficiency (Theory: + 177%)
Rs(4,5) = 5.45
Peak Width 5s(9) = 0.075
4
Rs = +50%
5
0
1
2
3
4
5
6
7
min
Colonna SB-C18 RRHT 4.6 x150mm
Massima lunghezza