Curriculum Vitae Prof. Raffaele A. Calogero AFFILIATION Name: Raffaele A. Calogero Date and place of birth: 3 Marzo 1960, Torino (Italia). Department: Department of Biotechnology and Health Sciences, University of Torino Address: Bioinformatics and Genomics Unit MBC Centro di Biotecnologie Molecolari Via Nizza 52, Torino 10126 Tel: ++39 0116706457 Fax: ++39 0112366457 Email: [email protected] www: http://www.bioinformatica.unito.it/ EDUCATION: 1984: Laurea in Biological Sciences summa cum laude from Naples University "Federico II" (Italy). 1984-­‐1985: Post-­‐laurea training at Institute of Genetics, General and Molecular Biology (Naples University "Federico II"). 1985-­‐1988: Fellowship at Max Plank Institute fuer Molekulare Genetik in Berlin (Germany). PROFESSIONAL EXPERIENCES: 1989-­‐1992: Researcher at SORIN Biomedica S.p.A. (I). 1992-­‐1998: Associate Professor of Molecular Biology, at Naples University "Federico II". 1998-­‐present: Associate Professor of Molecular Biology at University of Torino. CONSULTANCY ACTIVITIES: 2004-­‐2008: Consultant for the microarray program (AXXAM S.r.l, Milano, I) 2005-­‐2009: Consultant for the microarray program (Nerviano Medical Sciences S.r.l, Nerviano, MI, I) 2006-­‐today: Consultant for the microarray program (Laboratorio Platanias, Lurie Cancer Center, Northwestern University, Chicago, USA) 2010-­‐today: Consultant for Next generation sequencing application in QC (RBM SERONO, IVREA, I) RESEARCH INTERESTS: 1985 to 1988 As research fellow, At Max Plank Institute, R.A. Calogero was involved in studies concerning the mechanism of initiation of protein synthesis in E. coli. His work allows the definition of the importance of Shine Dalgarno sequence in protein synthesis initiation. 1989 to 1992 As researcher at SORIN Biomedica, R.A. Calogero has worked on protein expression and on the mechanisms involved in RNA-­‐protein interactions in viral genomes (HIV, HCV, HDV). He has set up a lab for protein expression in heterologous systems (Bacteria, Yeast, Insect cells) and purification. An important topic of his work was the expression in heterologous systems, purification of Hepatitis delta antigen and the study of his influence on the interaction with the viral genome. 1992 to 1998 As associate professor of Molecular Biology at Naples University, prof. Calogero has set up a molecular biology/bioinformatics lab at the Dept. of Genetics, Molecular and General Biology in Naples. The focus of the lab was the use of high-­‐throughput approaches to investigate the functionality of peptides able to interfere with the replication cycle of HIV-­‐1 and to develop innovative prokaryotic expression systems. The bioinformatics group was mainly involved in studies on transcription control. 1998-­‐present As associate professor at Medicine Faculty at Torino University, prof Calogero has set the Genomics and Bioinformatics unit (B&Gu). B&Gu is an interdisciplinary group devoted to the study of multifactorial diseases by mean of high throughput technologies -­‐ i.e. microarray, Next Generation Sequencing – and bioinformatics. B&Gu is part of the Tumor Immunology Group which has long been engaged in research on tumour immunogenicity. The immonology lab was established in 1977 by Guido Forni. Raffaele Calogero joined the group in 1999, when he moved from the Naples University Federico II, Italy. His background in molecular biology and bioinformatics and his consolidated experience in microarray data analysis and database mining have served to switch the laboratory’s classical immunological approach to a genome-­‐wide vision. The main focus of B&Gu are: Identification of Tumor-­‐associated Antigen to device lung/breast cancer broad and efficient immuno-­‐
prevention protocols. Modelling the pharmacological response to Cancer Stem Cells as adjuvant for DNA vaccination. Definition of control mechanisms of carcinogenesis modulated by miRNAs. Developing analytical methods for next generation sequencing applied to RNA-­‐seq and ChIP-­‐seq Furthermore, B&Gu is involved in research collaborations with university and industry for microarray and NGS data analysis and mining. PUBLICATIONS (2012-­‐2011) 1: Bolasco G, Calogero R, Carrara M, Banchaabouchi MA, Bilbao D, Mazzoccoli G, Vinciguerra M. Cardioprotective mIGF-­‐1/SIRT1 signaling induces hypertension, leukocytosis and fear response in mice. Aging (Albany NY). 2012 Jun;4(6):402-­‐16. PubMed PMID: 22691943; PubMed Central PMCID: PMC3409677. 2: Cordero F, Beccuti M, Donatelli S, Calogero RA. Large disclosing the nature of computational tools for the analysis of next generation sequencing data. Curr Top Med Chem. 2012;12(12):1320-­‐30. PubMed PMID: 22690679. 3: Galli GG, Honnens de Lichtenberg K, Carrara M, Hans W, Wuelling M, Mentz B, Multhaupt HA, Fog CK, Jensen KT, Rappsilber J, Vortkamp A, Coulton L, Fuchs H, Gailus-­‐Durner V, Hrabě de Angelis M, Calogero RA, Couchman JR, Lund AH. Prdm5 regulates collagen gene transcription by association with RNA polymerase II in developing bone. PLoS Genet. 2012 May;8(5):e1002711. Epub 2012 May 10. PubMed PMID: 22589746; PubMed Central PMCID: PMC3349747. 4: Lorenzato A, Martino C, Dani N, Oligschläger Y, Ferrero AM, Biglia N, Calogero R, Olivero M, Di Renzo MF. The cellular apoptosis susceptibility CAS/CSE1L gene protects ovarian cancer cells from death by suppressing RASSF1C. FASEB J. 2012 Jun;26(6):2446-­‐56. Epub 2012 Mar 2. PubMed PMID: 22389439. 5: Dani N, Olivero M, Mareschi K, van Duist MM, Miretti S, Cuvertino S, Patanè S, Calogero R, Ferracini R, Scotlandi K, Fagioli F, Di Renzo MF. The MET oncogene transforms human primary bone-­‐derived cells into osteosarcomas by targeting committed osteo-­‐progenitors. J Bone Miner Res. 2012 Feb 24. doi: 10.1002/jbmr.1578. [Epub ahead of print] PubMed PMID: 22367914. 6: Cordero F, Beccuti M, Arigoni M, Donatelli S, Calogero RA. Optimizing a massive parallel sequencing workflow for quantitative miRNA expression analysis. PLoS One. 2012;7(2):e31630. Epub 2012 Feb 20. PubMed PMID: 22363693; PubMed Central PMCID: PMC3282730. 7: Salvatori L, Caporuscio F, Verdina A, Starace G, Crispi S, Nicotra MR, Russo A, Calogero RA, Morgante E, Natali PG, Russo MA, Petrangeli E. Cell-­‐to-­‐cell signaling influences the fate of prostate cancer stem cells and their potential to generate more aggressive tumors. PLoS One. 2012;7(2):e31467. Epub 2012 Feb 6. PubMed PMID: 22328933; PubMed Central PMCID: PMC3273473. 8: Bolli E, Quaglino E, Arigoni M, Lollini PL, Calogero R, Forni G, Cavallo F. Oncoantigens for an immune prevention of cancer. Am J Cancer Res. 2011;1(2):255-­‐264. Epub 2010 Dec 20. PubMed PMID: 21969087; PubMed Central PMCID: PMC3180047. 9: Bosotti R, Carpinelli P, Healy S, Locatelli G, Cappella P, Lanfrancone L, Calogero R, Moll J, Isacchi A. Transcriptional analysis of the Aurora inhibitor Danusertib leading to biomarker identification in TP53 wild type cells. Gene. 2012 Feb 25;494(2):202-­‐8. Epub 2011 Sep 2. PubMed PMID: 21914463. 10: Baldi A, Piccolo MT, Boccellino MR, Donizetti A, Cardillo I, La Porta R, Quagliuolo L, Spugnini EP, Cordero F, Citro G, Menegozzo M, Calogero RA, Crispi S. Apoptosis induced by piroxicam plus cisplatin combined treatment is triggered by p21 in mesothelioma. PLoS One. 2011;6(8):e23569. Epub 2011 Aug 17. PubMed PMID: 21858171; PubMed Central PMCID: PMC3157425. 11: Riganti C, Pinto H, Bolli E, Belisario DC, Calogero RA, Bosia A, Cavallo F. Atorvastatin modulates anti-­‐
proliferative and pro-­‐proliferative signals in Her2/neu-­‐positive mammary cancer. Biochem Pharmacol. 2011 Nov 1;82(9):1079-­‐89. Epub 2011 Jul 23. PubMed PMID: 21803026. 12: Lenzken SC, Romeo V, Zolezzi F, Cordero F, Lamorte G, Bonanno D, Biancolini D, Cozzolino M, Pesaresi MG, Maracchioni A, Sanges R, Achsel T, Carrì MT, Calogero RA, Barabino SM. Mutant SOD1 and mitochondrial damage alter expression and splicing of genes controlling neuritogenesis in models of neurodegeneration. Hum Mutat. 2011 Feb;32(2):168-­‐82. doi: 10.1002/humu.21394. Epub 2011 Jan 25. PubMed PMID: 21120952.