Giada Rotunno, BSc
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Giada Rotunno
17/01/1986, Fondi (LT) (Italy)
Italian
UF Ematologia, University of Florence
Largo Brambilla 3, 50134 Florence, Italy
50134, Florence, Italy
+39 055 7947688
+39 055 7947688
[email protected]
Education
Current position: PhD student in Clinical and Experimental Oncology at the University of Florence Postdoctoral ship at the University of Florence, Department of Hematology, under the supervision of Professor
A.M. Vannucchi. The title of my project is “ASXL1 in myeloproliferative neoplasms: characterization of its
pathogenetic role and potential therapeutic significance”.
February 2012: Specialty Degree in Medical Biothecnology “Magna cum laude” at the University of Florence,
thesis title: “Analysis of MPL mutations in patients with MPN: methods and clinical correlations” under the
supervision of Professor AM.Vannucchi, Department of haematology, University of Florence.
April 2009: First-Degree 105/110 at University of Florence,thesis title “Intracellular redistribution of BCR/Abl
in deep Hypoxia” under the supervision of Professor Dello Sbarba, Department of Experimental Pathology
and Oncology.
April 2009-December 2012: Student fellowship in Department of Hematology, University of Florence, under
the supervision of Dr.Alterini Renato. The title of the research grant was “Immunohistochemistry analysis of
bone marrow biopsy in patients with mastocytosis”. We investigated bone marrow biopsy of both MDS and
MPN patients, a range of clonal haematological malignant diseases. In particular, I contribute to studies
focused on immunohistochemical and morphological alterations in bone marrow biopsy of patients with
Sistemic Mastocytosis and bone marrow of Systemic Sclerosis (SSc) patients and Multiple Sclerosis (SM)
patients undergoing autologous bone marrow transplantation.
Technical skills and competence
Next Generation Sequencing, Gene expression using Real Time PCR, sequencing analysis, High Resolution
Melting Analysis. Whole Genome Amplification. Cell coltures in liquid and semi-solid medium,Western
Blotting, Agarose gel, PCR, Dna/Rna/Protein extraction.Cytofluorimetric Analysis. Hystlogical Skills including
paraffin embedding od Bone Marrow Biopsy, Hematoxylin/Eosin staining , Silver-mpregnation, Pearls and
Immunohistochemistry analysis. Fluent English and basic knowledge of French and Spanish
Membership
2011: membership of the Italian Society of Experimental Hematology (SIES)
2010: AGIMM group (AIRC)
Publications
- Mannelli F, Bencini S, Peruzzi B, Cutini I, Sanna A, Benelli M, Magi A, Gianfaldoni G, Rotunno G, Carrai
V, Gelli AM, Valle V, Santini V,Notaro R, Luzzatto L, Bosi A. A systematic analysis of bone marrow cells by
flow cytometry defines a specific phenotypic profile beyond GPI deficiency in paroxysmal nocturnal
hemoglobinuria. Cytometry B Clin Cytom. 2013 Mar;84(2):71-81.doi: 10.1002/cyto.b.21064.
- Carrai V, Miniati I, Guiducci S, Capaccioli G, Alterini R, Saccardi R, Conforti ML, Rigacci L, Rotunno
G, Bosi A, Cerinic MM. Evidence for reduced angiogenesis in bone marrow in SSc: immunohistochemistry
and multiparametric computerized imaging analysis. Rheumatology (Oxford). 2012 Jun;51(6):1042-8. doi:
10.1093/rheumatology/ker447. Epub 2012 Jan 23.
- Barbui T, Thiele J, Passamonti F, Rumi E, Boveri E, Randi ML, Bertozzi I, Marino F, Vannucchi AM, Pieri
L, Rotunno G, Gisslinger H,Gisslinger B, Müllauer L, Finazzi G, Carobbio A, Gianatti A, Ruggeri M, Nichele
I, D'Amore E, Rambaldi A, Tefferi A. Initial bone marrow reticulin fibrosis in polycythemia vera exerts an
impact on clinical outcome. Blood. 2012 Mar 8;119(10):2239-41. doi: 10.1182/blood-2011-11-393819.
Epub 2012 Jan 13.
- E Rumi, D Pietra, P Guglielmelli, R Bordoni, I Casetti, C Milanesi, E Sant’Antonio, V Ferretti, A Pancrazzi,
G Rotunno, M Severgnini, A Pietrelli, C Astori, E Fugazza, C Pascutto, E Boveri, F Passamonti, G De Bellis,
A Vannucchi, and M Cazzola Acquired copy-neutral loss of heterozygosity of chromosome 1p as a molecular
event associated with marrow fibrosis in MPL-mutated myeloproliferative neoplasms. Blood. 2013 Apr 10.
[Epub ahead of print]
- Vannucchi AM, Lasho TL, Guglielmelli P, Biamonte F, Pardanani A, Pereira A, Finke C, Score J, Gangat
N, Mannarelli C, Ketterling RP, Rotunno G, Knudson RA,Chiara Susini M, Laborde RR, Spolverini
A, Pancrazzi A, Pieri L, Manfredini R, Tagliafico E, Zini R, Jones A, Zoi K, Reiter A, Duncombe A, Pietra
D, Rumi E,Cervantes F, Barosi G, Cazzola M, Cross NC, Tefferi A Mutations and prognosis in primary
myelofibrosis Leukemia. 2013 Apr 26. doi: 10.1038/leu.2013.119. [Epub ahead of print].