General Information Family name: Papale Name: Massimo Date of birth: August 20th1978 E-­‐mail: [email protected] Education 2002-­‐ Degree in medical biotechnology at ”Federico II” University of Naples; 2007: PhD in Molecular Medicine of Adult and Childhood at University of Foggia; 2012: Degree of Specialization in Clinical Pathology at “G. d'Annunzio” University of Chieti and Pescara. Current Position •
Technical Director at Apuliabitech S.c.a.r.l. a SME founded by public and private stakeholders with two main core businesses: ICT and Biotechnology. ICT encompasses the development of internet sites, databases and apps for mobiles with specific focus on the use of ICT for the improvement of the health care. Biotechnology activity regards the identification of confident biomarkers of renal diseases including renal cancer, their validation and set up of specific assays for their quantitation in biological fluids. Further, Apuliabiotech is proprietary of a patent for the Renal Progression Kit that is useful to predict the progression of renal damage in patients affected by Berger's disease. Apuliabiotech's mission includes: 1) Development of new prototypal assays to test the usefulness of the more promising biomarkers (discovered by the research programs currently on going at the Unit of Nephrology, dialysis and Transplantation of the DETO dept.) for the confident diagnosis of renal disease; 2) Prototypes’ patenting to protect the intellectual property; 3)Technology transfer to interested biotechnology Enterprises to obtain the commercialization of new diagnostic kits •
•
Member of the Scientific Committee (CTS) of the Center of Competence CE.R.T.A. S.c.a.r.l. (www. certa.it) Post Doc fellow at the Dept. of Surgery and Medical Sciences of the University of Foggia Scientific competences Massimo Papale has almost ten-­‐year experience in proteomic analysis of biological fluids. His research activity is focused, but not limited, to the identification of new biomarkers of renal diseases, including renal cancer, as documented by several original articles, reviews and book chapters published on peer-­‐reviewed journals on this topics Skills & Experience •
•
•
•
•
•
•
Definition of experimental plans for clinical proteomics studies Use of Proteomic tools (i.e two-­‐dimensional electrophoresis, protein profiling methods and mass spectrometry) to identify diagnostic and prognostic biomarkers of systemic and organ-­‐specific diseases. Use of Supervised statistical methods for the identification of multiparametric patterns correlated to human diseases In vitro studies to understand pathophysiological mechanisms of human disease Protection of the Intellectual Property (drafting and deposition of patent applications) Standardization of analytical protocols for Technology Transfer Project management Affiliations •
•
•
Italian Society of Nephrology (SIN); European Renal Association -­‐ European Dialysis and Transplantation Association (ERA-­‐EDTA); Italian Proteomic Association (ItPa) Honors and prizes From 2011: member of the group of Study on OMICS sciences of the Italian Society of Nephrology (http://www.sin-­‐italy.org/Soci_GdS/gruppi_omiche.asp) where He is national referent for the standardization of the guide lines for urine collection, processing and storage for proteomics analysis • From 2012: member of the scientific committee of the international Summer School of Renal Pathology (http://www.issrp.com/index.php?option=com_content&view=article&id=56&Itemid
=75&lang=en) and referent for the theme: OMICS sciences and Kidney. • 2012 “G. Montel” young researcher prize at University of Foggia (http://www.unifg.it/Premi-­‐di-­‐Ricerca-­‐Gianluca-­‐Montel-­‐2010 2011/Premiati/2185/) as best young researcher in medical sciences; •
Publications a) Original Articles 1) ROCCHETTI Maria Teresa, PAPALE Massimo, D'APOLLO Anna Maria, SURIANO Ida Valentina, DI PALMA Anna Maria, VOCINO Grazia, MONTEMURNO Eustacchio, VARRASO Leonarda, GRANDALIANO Giuseppe, DI PAOLO Salvatore, GESUALDO Loreto (2013). Association of urinary LG3 and free k-­‐light chains with disease activity and histological injury in IgA Nephropathy . Clin J Am Soc Nephrol. 2013 Jul;8(7):1115-­‐25 2) LO RUSSO Lucio , PAPALE Massimo, PERRONE D., RANIERI Elena, RUBINI, C., GIANNATEMPO G., SANTARELLI A., COLELLA G., LO MUZIO Lorenzo. Salivary proteomic signatures of oral squamous cell carcinoma. European Journal of Inflammation, vol. 10, issue 1, 2012, 61-­‐70 3) ROCCHETTI Maria Teresa, TAMMA Grazia, LASORSA D, SURINAO Ida Valentina, D'APOLLO Annamaria, PAPALE Massimo, MASTROFRANCESCO L, GRANDALIANO Giuseppe, SVELTO Maria, VALENTI Giovanna, GESUALDO Loreto, Di PAOLO Salvatore. Altered urinary excretion of Aquaporin-­‐2 in IgA Nephropathy. Eur J Endocrinol. 2011 Oct;165(4):657-­‐64 4) PAPALE Massimo, DI PAOLO Salvatore, MAGISTRONI Riccardo, LAMACCHIA Olga, DI PALMA Anna Maria, DE MATTIA Angela, ROCCHETTI Maria Teresa, FURCI Luciana, PASQUALI Sonia, DE COSMO Salvatore, CIGNARELLI Mauro and GESUALDO Loreto (2010). Urine Proteome Analysis May Allow Non-­‐Invasive Differential Diagnosis of Diabetic Nephropathy. DIABETES CARE, vol. 33(11); p. 2409-­‐2415, ISSN: 0149-­‐5992, doi: 10.2337/dc10-­‐0345 5) CASSANO Silvana, AGNESE Savina, D'AMATO Vittorio, PAPALE Massimo, GARBI Corrado, CASTAGNOLA Paolo, RUOCCO Maria Rosaria , CASTELLANO Ivan, DE VENDITTIS Enrico, SANTILLO Maria, AMENTE Stefano, PORCELLINI Antonio, AVVEDIMENTO Enrico Vittorio (2010). Reactive oxygen species, Ki-­‐Ras, and mitochondrial superoxide dismutase cooperate in nerve growth factor-­‐induced differentiation of PC12 cells. THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 285 (31); p. 24141-­‐53, ISSN: 0021-­‐9258, doi: 10.1074/jbc.M109.098525 6) ROCCHETTI Maria Teresa, CENTRA Marta, PAPALE Massimo, BORTONE Grazia, PALERMO Carmen, CENTONZE Diego, RANIERI Elena, DI PAOLO Salvatore and GESUALDO Loreto (2008). Urine protein profile of IgA nephropathy patients may predict the response to ACE-­‐Inhibitors therapy PROTEOMICS, vol. 8; p. 206-­‐216, ISSN: 1615-­‐9853, doi: 10.1002/pmic.200700492 7) POVERO Giovanni, PAPALE Massimo, GESUALDO Loreto, ALPI Amedeo, PERATA Pierdomenico and LORETI Elena (2010). Identification of Grapevine Cultivar Biomarkers Using Surface-­‐Enhanced Laser Desorption and Ionization (SELDI-­‐TOF-­‐MS). AMERICAN JOURNAL OF ENOLOGY AND VITICULTURE, vol. 61:4; p. 492-­‐497, ISSN: 0002-­‐9254, doi: 10.5344/ajev.2010.10010 8) PAPALE Massimo, PEDICILLO Maria Carmela, THATCHER Bradley J., DI PAOLO Salvatore, LO MUZIO Lorenzo, BUFO Pantaleo, ROCCHETTI Maria Teresa, CENTRA Marta, ELENA RANIERI Elena and GESUALDO Loreto (2007). Urine profiling by SELDI-­‐
TOF/MS: Monitoring of the critical steps in sample collection, handling and analysis. JOURNAL OF CHROMATOGRAPHY. B, vol. 856; p. 205-­‐213, ISSN: 1570-­‐0232 9) PAPALE Massimo, PEDICILLO Maria Carmela, DI PAOLO Salvatore, THATCHER B radleyJ, LO MUZIO Lorenzo, BUFO Pantaleo, ROCCHETTI Maria Teresa, CENTRA Marta, RANIERI Elena and GESUALDO Loreto (2008). Saliva analysis by Surface Enhanced laser Desorption/ Ionization Time of Flight Mass Spectrometry (SELDI-­‐TOF/MS): from sample collection to data analysis. CLINICAL CHEMISTRY AND LABORATORY MEDICINE, vol. 46(1); p. 89-­‐99, ISSN: 1434-­‐6621, doi: 10.1515/CCLM.2008.013 b) Reviews 1) PAPALE M, Di Paolo S et al. PROTEOMICS AND DIABETIC NEPHROPATHY: WHAT HAVE WE LEARNED FROM A DECADE OF CLINICAL PROTEOMICS STUDIES? J Nephrol. 2014 Feb 25. [Epub ahead of print] 2) Massimo PAPALE, Maria Teresa Rocchetti, Loreto Gesualdo CLINICAL PROTEOMICS: THE POTENTIALITY OF URINE ANALYSIS FOR UNDERSTANDING DIABETIC NEPHROPATHY; EMJ Neph. 2013;1:32-­‐39 3) NETTI Giuseppe Stefano, ROCCHETTI Maria Teresa, PAPALE Massimo, CENTRA Marta, CENTONZE Diego, DI PAOLO Salvatore, RANIERI Elena and GESUALDO Loreto (2008). Proteomica e Rene: Un approccio innovativo allo studio delle malattie Renali. GIORNALE ITALIANO DI NEFROLOGIA, vol. 25 (2); p. 169-­‐182, ISSN: 0393-­‐5590. 3) Book Chapters 1) PAPALE Massimo, ROCCHETTI Maria Teresa. Proteomic Approaches by SELDI and MALDI-­‐TOF/MS for CTL Analysis. Methods Mol Biol. 2014;1186:233-­‐42. doi: 10.1007/978-­‐1-­‐4939-­‐1158-­‐5_12 2) ROCCHETTI Maria Teresa, PAPALE Massimo, GESUALDO Loreto. Two-­‐Dimensional Gel Electrophoresis Approach for CTL Phosphoproteome Analysis. Methods Mol Biol. 2014;1186:243-­‐51. doi: 10.1007/978-­‐1-­‐4939-­‐1158-­‐5_13. 3) ROCCHETTI Maria Teresa, PAPALE Massimo and GESUALDO Loreto. Urine Protein Profiling in IgA Nephropathy. US NEPHROLOGY. vol. 5, issue 2, T O U C H B R I E F I N G S 4) DADDABBO Annarita, PAPALE Massimo, DI PAOLO Salvatore, MAGALDI Simona, COLELLA Roberto, d’ONOFRIO Valentina, DI PALMA Anna Maria, RANIERI Elena, GESUALDO Loreto and ANCONA Nicola (2008). SVD Based Feature Selection and Sample Classification of Proteomic Data. In: SPRINGER BERLIN HEIDELBERG. Knowledge-­‐Based Intelligent Information and Engineering Systems. vol. 5179/2008, p. 556-­‐563, ISBN/ISSN: 978-­‐3-­‐540-­‐85566-­‐8