Curriculum Vitae
Antonella Tramutola
Born at Potenza, 20/06/1984
Resident: ROMA
Italy
e-mail address: [email protected]
[email protected]
RESEARCH AND WORKIN’ EXPERIENCE:
June 2014- to data Dept. of Biochemistry, “Sapienza” University – Rome (Italy)
PostDoc in Biochemistry, laboratory of Prof. Perluigi M. workin’ on “Molecular Mechanism
involved in neurodegenerative diseases”.
March 2014- to December 2014 Dept. of Chemistry, University of Kentucky –Lexington
(USA)
PostDoctoral Collaboration in Chemistry, Laboratory of D.A. Butterfield workin’ on
“Molecular Mechanism involved in Alzheimer Disease”
June 2013 – to May 2014 Dept. of Biochemistry, “Sapienza” University – Rome (Italy)
PostDoc in Biochemistry, laboratory of Prof. Perluigi M. workin’ on “Molecular Mechanism
involved in neurodegenerative diseases”.
Jan. 2010- Apr. 2013 Dept of Pharmacology, Catholic University Medical School - Rome
(Italy)
PhD studentship in Neuroscience, laboratory of Prof. Navarra P. and Dott.ssa Dello Russo C.,
workin’ on “Modification of corticosterone levels during neonatal life on rats: long term effects on
inflammatory processes of Central Nervous System (Neuroinflammation)” and “Modulatory effects
of the antiretroviral drugs on microglial pro- inflammatory activation elicited by gp120”.
Jan. 2008 – Dec. 2009- Dept. of Human Physiology and Pharmacology “V. Erspamer",
“Sapienza” University – Rome (Italy)
Internship for the achievement of the experimental thesis in Pharmacology and Pharmacotherapy,
laboratory of Dott.ssa Casolini P. and Dott.ssa Catalani A., workin’ on “ Effects of Zidovudine
during perinatal life: biochemistry and behavioral effects on mice” and “Maternal corticosterone
effects on hypothalamus-pituitary-adrenal axis regulation and behavior of the offspring in rodents”.
Oct. 2009 – Jun. 2010- Dept. of Neurological Science, “Sapienza” University – Rome (Italy)
Scholar ship for cooperation-work in Library of Neurological Science
Sept. 2006 – Feb. 2007- Neuroscience Center, University of Helsinki, FI-00014 Helsinki
(Finland)
Internship for the achievement of the experimental thesis in Neuroscience, laboratory of Prof. Taira
T., workin’ on “New pharmacologic animal model: testing the effect of Eserine, Tetrodotoxin and
high potassium concentration on cholinergic transmission of Abdominal Ganglia (A6) of
Periplaneta Americana”.
EDUCATIONAL BACKGROUND:
June 2013-to data:
Dept. of Biochemistry, “Sapienza” University- Rome (Italy)
Post Doctoral Position
Project Title: “ Molecular Mechanism involved in neurodegenerative diseases”
January 2013- November 2013:
Catholic University Medical School - Rome (Italy)
Master in “ Preclinical and Clinical Development of Drug”.
Project Title :” Evaluation of innovation drugs”. Tutor: D.ssa Maggini Marina, ISS,
Pharmacoepidemiology field.
November 2009 – October 2012
Dept of Pharmacology, Catholic University Medical School - Rome (Italy)
PhD student in Neuroscience
Project title: “In vivo and in vitro studies on stress, stress related pathologies and
neuroinflammation”
Tutor: Prof. Navarra Pierluigi, Pharmacology Institute of UCSC
Research fields:
 Modulatory effects of the antiretroviral drugs on microglial pro- inflammatory activation
elicited by gp120.
 Maternal exposure to low levels of corticosterone during lactation increases
neuroinflammatory response after administration of LPS
July 2010 - Admitted to Italian Register of Biologist
October 2009 - “Sapienza” University – Rome (Italy)
Master’s degree in Neurobiology (LM 06), summa cum laude
Thesis Title: “Exposure to Zidovudine during intrauterine and neonatal life: biochemistry and
behavioral effects on mice”.
Supervisor:
─ Dr. ssa Casolini Paola, Dept of Human Physiology and Pharmacology “V. Erspamer",
“Sapienza” University – Rome (Italy).
─ Dr. Mele Andrea, Dept. of Genetic and Molecular Biology,
“Sapienza” University, Rome (Italy).
November 2007- University of Perugia (Italy)
Bachelor’s degree in Biological Science (L13)
Thesis Title: “Effect of Eserine, Tetrodotoxin and high potassium concentration on cholinergic
transmission of Abdominal Ganglia (A6) of Periplaneta Americana”.
Supervisor:
─ Dott.ssa Marsili Valeria, Dept of Environment and Cellular Biology, University of Perugia
(Italy).
─ Prof. Taira Tomi, Neuroscience Center, University of Helsinki, FI-00014 Helsinki (Finland).
September 2006- Erasmus Project
Scholarship of 6 months for Erasmus Project, University of Helsinki (Finland).
July 2002- Liceo Classico “Q.O. Flacco” Potenza (Italy)
High School diploma
PROFESSIONAL SOCIETIES
Society of Neuroscience (SFN)
Italian Society of Pharmacology (SIF)
TECHNICAL SKILLS
Surgery and Tissue Processing :
Extensive experience with Small Animal Surgery, Brain Dissection, Histochemistry (Immuno
Histochemistry, BrdU)
Behavioral Training:
Water maze, Plus maze, Maternal behaviour, Play behaviour and Stress related behaviour, EAE
scoring
Cell culture:
Cell Lines (C6, SHSY5, C33A, HeLa, SiHa), Primary culture of Microglia and Astrocytes.
Molecular Biology:
RNA and DNA extraction, RT-PCR, Real Time PCR, Western Blot analysis, Proteomics and Redox
Proteomics.
Other Methods:
ELISA , EIA, RIA of corticosterone, Reactive oxygen species assays and cytotoxicity assays.
Computer skills
Microscoft Windows, Office pack, Statistic Analisys: NCSS, Biopac-Student system, Sygma Plot
and Graph Pad , Images processing and analysis: Image J and Work out software.
MOTHER TONGUE
ITALIAN
FOREIGN LANGUAGES
ENGLISH GOOD (WRITTEN AND SPOKEN)
July 1998 – British School, Colchester
English language course at the “Colchester language school”
OTHER INFORMATIONS:
Italian Driver's licence B
April 2007
Fellowship from Music Academy “European center of Tuscolano”, direct by Mogol.
PUBLICATIONS:
Tramutola A, Triplett JC, Di Domenico F, Niedowicz DM, Murphy MP, Coccia R, Perluigi
M, Butterfield DA. Alteration of mTOR signaling occurs early in the progression of
Alzheimer disease (AD): analysis of brain from subjects with pre-clinical AD,
amnestic mild cognitive impairment and late-stage AD. J Neurochem. 2015 Jan 27.
doi: 10.1111/jnc.13037.
Perluigi M, Pupo G, Tramutola A, Cini C, Coccia R, Barone E, Head E, Butterfield DA, Di
Domenico F. Neuropathological role of PI3K/AKT/mTOR axis in Down syndrome
brain. BBA - Molecular Basis of Disease. 2014 April 8.
Di Domenico F, Pupo G, Tramutola A, Giorgi A, Eugenia Schininà M, Coccia R, Head E,
Allan Butterfield D, Perluigi M. Redox proteomics analysis of HNE-modified proteins
in Down syndrome brain: clues for understanding the development of Alzheimer
disease. Free Radic Biol Med. 2014 Mar 25.
Lisi L, Tramutola A, Navarra P, Dello Russo C. Antiretroviral agents increase NO
production in gp120/IFNγ-stimulated cultures of rat microglia via an arginasedependent mechanism. J Neuroimmunol. 2014 Jan 15; 266(1-2):24-32.
Zuena AR, Giuli C, Venerosi Pesciolini A, Tramutola A, Ajmone-Cat MA, Cinque C, Alemà
GS, Giovine A, Peluso G, Minghetti L, Nicolai R, Calamandrei G, Casolini P.
Transplacental exposure to AZT induces adverse neurochemical and
behavioraleffects in a mouse model: protection by L-acetylcarnitine. PLoS One.
2013;8(2):e55753. Erratum in: PLoS One. 2013; 8(10).
Cinque C, Zuena AR, Catalani A, Giuli C, Tramutola A, Scaccianoce S. Maternal
exposure to low levels of corticosterone during lactation increases social play
behavior in rat adolescent offspring. Rev Neurosci. 2012; 23(5-6):723-30.
Lisi L, Tramutola A, De Luca A, Navarra P, Dello Russo C. Modulatory effects of the
CCR5 antagonist maraviroc on microglial pro-inflammatory activation elicited by
gp120. J Neurochem. 2012 Jan; 120(1):106-14.
De Corato A, Lisi L, Capuano A, Tringali G, Tramutola A, Navarra P, Dello Russo C.
Trigeminal satellite cells express functional calcitonin gene-related peptide receptors,
whose activation enhances interleukin-1β pro-inflammatory effects. J Neuroimmunol.
2011 Aug 15; 237(1-2):39-46.
SELECTED ABSTRACTS
(NATIONAL AND INTERNATIONAL MEETINGS):
Butterfield D.A., Di Domenico F., Sultana R., Fiorini A., Tramutola A., Cenini G., Head E. and
Perluigi M. “Proteomics Insight into Molecular Mechanisms Engaged Prior to and Following
Development of Alzheimer Disease Neuropathology in Down Syndrome Brain: Relevance to
Oxidative Stress and Neuropathology of both DS and AD” (November 2014, Seattle, USA)
Pupo G., Tramutola A., Foppoli C., Di Domenico F. “Pathological role of PI3K/AKT/mTOR
signaling pathway in Down Syndrome brain”. 57th National Meeting of Italian Society of
Biochemistry and Molecular Biology (September 2013, Ferrara, Italy).
Lisi L., Tramutola A., Dello Russo C., Navarra P. “Modulatory effects of antiretroviral drugs on
microglial pro-inflammatory activation”. 42th Society for Neuroscience (SFN) Annual Meeting
(October 2012, New Orleans, USA)
Giuli C., Cinque C., Di Meglio A., Silletti V., Zuena A.R. , Tramutola A., Alemà G.S., Mennuni
G., Casolini P., Nicoletti F., Catalani A., Mairesse J. “Sex difference in the effects of maternal
exposure to low levels of corticosterone during lactation on the circadian HPA axis activity and
sleep homeostasis of the adult progeny”. 8th International Brain Research Organization (IBRO)
World Congress of Neuroscience (July 2011, Florence, Italy).
Tramutola A., Alemà G.S., Casolini P., Catalani A., Cinque C., Giuli C. , Mennuni G., Silletti V. ,
Sparago M. , Zuena A.R. and Mairesse J. “Effects of maternal exposure to low levels of
corticosterone during lactation on sleep/wake cycle and hippocampal neurogenesis in the adult
progeny”. 40th Society for Neuroscience (SFN) Annual Meeting (November 2010, San Diego,
USA)
Giuli C., Alemà G.S., Casolini P., Catalani A., Cinque C., Tramutola A., Venerosi A.,. Zuena A.
R, Calamandrei G. “Cognitive impairment and glutamate receptors modification in mice exposed to
zidovudine during intrauterine life”. 40th Society for Neuroscience (SFN) Annual Meeting
(November 2010, San Diego, USA)
Zuena A., Alemà G. S., Casolini P., Cinque C., Di Meglio A., Giuli C., Scaccianoce S., Sparago M.
L., Tramutola A., Catalani A. “Maternal exposure to moderate doses of corticosterone during
lactation induces behavioral and neuroendocrine effects across generations”. 40th Society for
Neuroscience (SFN) Annual Meeting (November 2010, San Diego, USA)
Data23 4 2015
Antonella Tramutola
I authorise the processing of my personal data for personal research and selection
purposes under D. Lgs. n.196 of 30/06/2003.