Molecular profiles of cancer stem-like cell populations in aggressive thyroid cancers
Journal Name: Endocrine
Mariavittoria Dima1§, Valeria Pecce1§, Mauro Biffoni2, Cira Rosaria Tiziana Di Gioia3, Giovanni Tallini4, Marco Biffoni5, Francesca Rosignolo1, Antonella
Verrienti1, Marialuisa Sponziello1, Giuseppe Damante6, Diego Russo7, Cosimo Durante1
§
These authors contributed equally to this work
Corresponding author
Diego Russo, MD
Dipartimento di Scienze della Salute
Università di Catanzaro “Magna Graecia”
Viale Europa - 88100 Catanzaro - ITALY
E-mail: [email protected]
Supplementary Table 2 EMT-related genes expression data in Thyroid cancer cell lines
Gene
Cell Line 1
Cell Line 2
Cell Line 3
P value
Primary
Thyrospheres
Cell Line 4
P value
Primary
Thyrospheres
P value
Primary
Thyrospheres
P value
Primary
Thyrospheres
EMT-related genes
CDH11
1 ± 0.15
0.66 ± 0.00
0.080
1 ± 0.13
1.05 ± 0.08
0.732
1 ± 0.32
1.19 ± 0.04
0.538
1.00 ± 0.10
1.21 ± 0.11
0.188
CDH16
1 ± 2.12
0.44 ± 0.24
0.463
/
/
/
/
/
/
/
/
/
CDH1
1 ± 0.82
4.11 ± 1.21
0.103
1 ± 0.81
0.22 ± 0.00
0.245
1 ± 0.31
8.71 ± 2.52
0.051
1.06 ± 0.52
0.17 ± 0.23
0.155
CDH2
1 ± 0.00
1.62 ± 0.02
0.001
1 ± 0.17
1.94 ± 0.11
0.022
1 ± 0.07
0.90 ± 0.01
0.174
1.00 ± 0.08
1.11 ± 0.07
0.289
COL5A1
1 ± 0.16
1.89 ± 0.35
0.082
1 ± 0.18
3.11 ± 0.47
0.027
1 ± 0.08
2.40 ± 0.25
0.017
1.00 ± 0.07
4.85 ± 0.87
0.025
ECM1
1 ± 0.06
3.84 ± 0.24
0.004
1 ± 0.20
3.15 ± 0.52
0.032
1 ± 0.07
2.92 ± 0.07
0.001
1.01 ± 0.18
0.41 ± 0.02
0.044
FN1
1 ± 0.06
18.63 ± 0.00
0.000
1 ± 0.00
8.43 ± 0.31
0.001
1 ± 0.03
6.62 ± 0.20
0.001
1.00 ± 0.03
6.70 ± 0.62
0.006
KLF6
1 ± 0.08
4.83 ± 0.33
0.004
1 ± 0.03
10.08 ± 1.02
0.006
1 ± 0.04
3.49 ± 0.23
0.005
1.00 ± 0.08
3.17 ± 0.14
0.003
LAMB3
LOX
1 ± 0.01
1 ± 0.00
10.80 ± 0.77
0.81 ± 0.18
0.003
0.276
1 ± 0.16
1 ± 0.07
10.62 ± 1.43
0.90 ± 0.00
0.011
0.200
1 ± 0.05
1 ± 0.03
34.74 ± 3.89
0.26 ± 0.02
0.007
0.001
1.01 ± 0.21
1.01 ± 0.16
7.95 ± 1.06
0.26 ± 0.01
0.012
0.021
LOXL2
LPHN2
1 ± 0.05
1 ± 0.03
0.21 ± 0.00
2.25 ± 0.03
0.002
0.001
1 ± 0.15
1 ± 0.09
1.72 ± 0.14
5.29 ± 0.05
0.038
0.000
1 ± 0.05
1 ± 0.07
0.62 ± 0.09
2.51 ± 0.02
0.033
0.001
1.01 ± 0.17
1.00 ± 0.06
0.35 ± 0.07
1.16 ± 0.07
0.038
0.135
MMP1
1 ± 0.12
252.22 ± 2.84
0.000
1 ± 0.08
234.31 ± 10.68
0.001
1 ± 0.19
4.99 ± 1.36
0.055
1.00 ± 0.14
25.42 ± 5.40
0.024
MMP2
1 ± 0.45
6.50 ± 0.40
0.006
1 ± 0.17
10.80 ± 2.80
0.039
1 ± 0.03
2.31 ± 0.70
0.117
1 ± 0.18
5.17 ± 0.84
0.021
MMP9
1 ± 1.37
41.70 ± 7.68
0.018
/
/
/
/
/
/
1 ± 0.32
2.47 ± 1.01
0.194
NID1
PLAU
1 ± 0.03
1 ± 0.07
0.40 ± 0.02
1.25 ± 0.02
0.002
0.043
1 ± 0.01
1 ± 0.09
2.63 ± 0.38
2.94 ± 0.05
0.027
0.001
1 ± 0.04
1 ± 0.07
4.28 ± 0.22
1.29 ± 0.10
0.002
0.081
1.00 ± 0.03
1.00 ± 0.04
3.61 ± 0.48
1.84 ± 0.32
0.016
0.066
PLAUR
1 ± 0.15
27.03 ± 0.69
0.000
1 ± 0.05
82.60 ± 0.36
0.000
1 ± 0.14
29.67 ± 4.25
0.011
1.00 ± 0.05
12.81 ± 1.10
0.004
SNAI1
SNAI2
1 ± 0.12
1 ± 0.07
2.11 ± 0.29
2.50 ± 0.83
0.039
0.127
1 ± 0.71
1 ± 0.22
15.00 ± 2.70
6.12 ± 1.25
0.020
0.030
1 ± 0.08
1 ± 0.08
7.88 ± 0.01
2.66 ± 0.24
0.000
0.011
1.00 ± 0.07
1.01 ± 0.23
3.60 ± 0.24
0.51 ± 0.09
0.005
0.101
SNAI3
1 ± 0.38
3.41 ± 1.77
0.205
/
/
/
1 ± 0.89
4.73 ± 5.43
0.458
/
/
/
SPP1
1 ± 0.84
10.90 ± 1.17
0.011
1 ± 0.58
27.32± 7.07
0.035
1 ± 0.31
85.87 ± 1.01
0.000
1.00 ± 0.00
2.71 ± 0.63
0.061
SPRY4
STAT3
1 ± 0.60
1 ± 0.01
15.79 ± 0.68
2.68 ± 0.04
0.002
0.000
1 ± 0.27
1 ± 0.06
33.29 ± 5.78
5.55 ± 0.99
0.016
0.023
1 ± 0.52
1 ± 0.02
8.94 ± 0.38
6.80 ± 0.41
0.003
0.003
1.02 ± 0.29
1.00 ± 0.07
11.87 ± 1.23
2.27 ± 0.60
0.007
0.097
TCF3
1 ± 0.08
1.38 ± 0.03
0.024
1 ± 0.14
15.54 ± 0.64
0.001
1 ± 0.10
7.57 ± 0.46
0.003
1.00 ± 0.04
5.51 ± 1.00
0.024
Gene
Cell Line 1
Cell Line 2
Cell Line 3
P value
Primary
Thyrospheres
TIMP3
1 ± 0.07
1.82 ± 0.44
TWIST1
TWIST2
1 ± 0.01
1 ± 0.53
VIM
P value
Primary
Thyrospheres
0.121
1 ± 0.01
4.14 ± 0.04
5.95 ± 1.18
2.36 ± 1.22
0.027
0.304
1 ± 0.12
1 ± 0.18
1 ± 0.02
2.73 ± 0.01
0.000
ZEB1
1 ± 0.26
13.59 ± 1.83
ZEB2
FBLN1
1 ± 0.11
1 ± 0.21
GATA3
P value
Primary
Thyrospheres
0.000
1 ± 0.04
2.32 ± 0.06
7.33 ± 0.40
30.88 ± 7.07
0.002
0.027
1 ± 0.06
1 ± 0.28
1 ± 0.08
10.73 ± 2.10
0.023
0.011
1 ± 0.06
13.43 ± 1.82
5.47 ± 0.80
0.22 ± 0.05
0.016
0.034
1 ± 0.10
1 ± 0.12
18.99 ± 4.49
0.45 ± 0.07
1 ± 0.02
4.71 ± 0.32
0.004
/
/
LGALS1
1 ± 0.00
1.53 ± 0.18
0.053
1 ± 0.08
2.77 ± 0.12
LUM
1 ± 0.39
11.90 ± 2.91
0.035
1 ± 0.30
1.58 ± 0.06
MUC4
/
/
/
/
/
SATB1
1 ± 0.26
1 ± 0.14
5.95 ± 0.35
6.71 ± 0.45
0.004
0.003
1 ± 0.40
1 ± 0.17
9.57 ± 0.62
1.51 ± 0.30
SERPINE2
Cell Line 4
P value
Primary
Thyrospheres
0.001
1.00 ± 0.09
2.12 ± 0.15
0.012
8.48 ± 0.22
15.18 ± 6.34
0.000
0.087
1.00 ± 0.07
1.05 ± 0.47
4.10 ± 0.74
17.09 ± 10.35
0.028
0.160
1 ± 0.03
3.78 ± 0.38
0.009
1.00 ± 0.05
1.41 ± 0.23
0.133
0.011
1 ± 0.07
7.19 ± 0.10
0.000
1.00 ± 0.03
5.11 ± 0.58
0.010
0.030
0.028
1 ± 0.11
1 ± 0.03
8.08 ± 0.08
1.13 ± 0.20
0.000
0.452
1 ± 0.21
1 ± 0.09
4.55 ± 0.15
0.56 ± 0.07
0.003
0.031
1 ± 0.09
3.91 ± 0.06
0.001
1 ± 1.20
1.52 ± 0.24
0.829
0.003
1 ± 0.14
1.70 ± 0.06
0.023
1 ± 0.00
0.94 ± 0.05
0.198
0.120
1 ± 0.37
1.21 ± 0.25
0.630
1 ± 0.11
0.72 ± 0.08
0.099
1 ± 0.00
4.30 ± 2.28
0.177
/
/
/
1 ± 0.22
1 ± 0.15
9.83 ± 0.27
1.15±0.07
0.001
0.347
1 ± 0.40
1 ± 0.04
9.49 ± 1.85
2.04 ± 0.41
0.024
0.070
0.004
0.177
Data are expressed as means ± SD. Bold values indicate the common significant genes in all the cell lines analyzed.