Supporting Fig. 1
A
B
**
con
5
con
con
Proliferation (folds)
miR-155 mimics
**
3
2
1
0
5
miR-155 mimics
4
4
miR-155 mimics
**
U2OS
4
U2OS
3
3
**
2
SaOS2
**
2
1
0
24
48
72
0
24
Time (h)
C
D
1.2
G2/M
S
G0/G1
1
0.8
Ki-67
0.6
β-tubulin
0.4
conmimics conmimics
0.2
0
U2OS
con mimics
U2OS
**
SaOS2
1
Cell cycle distribution
Relative miR-155 level
5
con mimics
SaOS2
SaOS2
48
72
Supporting Fig. 2
A
B
**
3
con
miR-155 mimics
**
2.5
2
1.5
1
0.5
Relative TOPflash activity
Relative TOPflash activity
3.5
4
U2OS
3.5
**
3
U2OS
SaOS2
4.5
SaOS2
control
miR-155 mimics
4
**
**
2.5
3.5
**
3
**
**
2.5
2
2
1.5
1.5
1
1
0.5
0.5
0
0
control
miR-155 mimics
0
Axin2
CD44
Lgr5
Axin2
CD44
Lgr5
Supporting Fig. 3
con
luc-HBP1-3UTR
Relative luciferase activity
luc-HBP1-3UTR-M
1.4
U2OS
1.2
1
0.8
0.6
**
0.4
0.2
0
Control
miR-155