IAMC – Istituto Sperimentale
Talassografico
CNR – Messina, Italy
~~~~
Università degli studi di
Messina, Italy
WP3 - WP4 – WP5
State of the Art October 2002
L’Atalante
Urania
1.453
AUI
1.420
ALI
0
500
1000
1500
13.358
5.136
UUI
ULI
2.238
ABB
0
2500
5000
10000
Discovery
385
DUI
1.698
BI
911
DLI
6.228
UBB
2000
Bannock
15000
1.578
BBB
435
DBB
1500
1550
1600
1650
1700
0
500
1000
Total count cells/ml (x1000)
ALI
0
35,6661 3
8,505
1 46,286
A BB
0%
98,76774
50%
10013,952
UUI
6219,2813
U B B 1275,75
7484,4
100%
0%
20%
1719,9
Salinity (%)
15,6
434,7
18,03
387,45
21,1
453,6
0%
20%
40%
60%
80% 100%
0%
625,52903
20%
40%
60%
80%
DB B
232,47
1 4,1 75
1 47,42
5
100%
0%
727,65
705
703,08
60%
362,88
1 04,895
50%
100%
Bannock Interface (BD-2002)
982,8
40%
BBB
112
277,83
Eubatteri % Archea %
Urania Interface (BD-2002)
3,7
515,97
BI
U L I717,60938
DUI
1048,95
80%
7.523,91
4,6
Salinity (%)
374,22
A UI
11
2.186,39
3.312,42
21 969,57
5.949,56
258,51
100%
0%
6.390,56
299,11
20%
40%
60%
80%
100%
Natural Sample of L’Atalante Interface (2001)
MERTZ 21_CM_101
KTK-14, Aj133615
Desulfobacula toluolica
Env. Smpl. Shaban Deep, Red Sea
Env. WCHB53
Epsilon proteobacteria, Ab013262
Desulfobacterium phenolicum
Unc. Spnge symbiont
L’Atalnte
Interface
natural
sample
Actinobacteria High GC
Candidate division KB1
Delta-Proteobacteria
SB1 - Firmicutes Low
CFBGC
related
Epsilon-Proteobacteria
Delta Proteobacteria
Actinobacteria
Unc. Delta proteobact., AF154102
KTK 14
KTK 14
Unid, AB015534
Unc. Spnge symbiont
Delta Proteobacteria
Candidate division KB1
Candidate division KB1
Firmicutes low GC
Actinobacteria related
Unc. Delta proteobact.
KTK 14, Aj133615
Unid, AB015534
Desulfobacterium phenolicum
Epsilon proteobacteria
MERTZ 21_CM_101
Desulfobacula toluolica
Delta Proteobacteria
Candidate division KB1
Firmicutes low GC
Delta Proteobacteria
Epsilon-Proteobacteria
Actinobacteria High GC
Delta-Proteobacteria
Enrichment of dilution (2001)
Urania
Interface
Bannock
Interface
L’Atalante
Body Brine
Oceanospirillum pusillum
Gamma proteobacteria
Uncultured hydrocarbon seep bacterium
Epsilon proteobacteria
Arcobacter sp.
Epsilon proteobacteria
Halomonas variabilis
Gamma proteobacteria
Oceanospirillum pusillum
Gamma proteobacteria
Unculturead Archeon
Archea
Alteromonas sp.
Gamma proteobacteria
Marinobacter hydrocarbonoclasticus
Gamma proteobacteria
Thiovulum sp.
Epsilon proteobacteria
Marinobacter aqueoli
Gamma proteobacteria
Alteromonadaceae bacterium LA34A
Gamma proteobacteria
Pseudomonas sp.
Gamma proteobacteria
Desulfotomaculum thermobenzoicum
Thermoanaerobacteriaceae
Alteromonas sp.
Gamma proteobacteria
Bannock Interface on P1
Bannock Interface on P1
Urania Interface on P10 (Petroleum)
Urania Interface on P10 (petroleum)
Urania Interface on P3
Bannock Interface
327 Alteromonadaceae
326 Marinobacter aqueoli
324
325
352
345
347
132
0
P1
0
135
Alteromonadaceae
Alteromonadaceae bacterium
Alteromonadaceae
Marinobacter h.
Marinobacter hydrocarbonoclasticus.
335
Idiomarina loihiensis strain 53
359
Pseudomonas pseudoalcaligenes
348
Idiomarina loihiensis strain 53
P4
0
P9
140
321
Alcanivorax borkumensis
322
Alteromonas bacterium LA34A
Urania Interface
359 Pseudomonas pseudoalcaligenes
P10 ( Petroleum )
Urania Body Brine
366 Alcanivorax venusti
P10 ( Petroleum )
365 Sulfitobacter sp. EE-36
364 Sulfitobacter sp. EE-36
Urania Body Brine on P3
368
Uncultured Halomonas
367
Sulfitobacter sp.
369
Pseudomonas alcalophila
P3
L’Atalante Upper Interface
350 Alteromonadaceae
340 Alteromonas sp.
353 Marinobacter h.
0
48
342
P1
231 Marinobacter h.
355 Alteromonadaceae b.
L’Atalante Body Brine
361 Halomonas m.
P10 (petroleum)
362 Idiomarina l.
363 Alcanivorax v.
UBB P3,P10
BI P4
ABB P10
BI P1
BI P9
AI P1
UI P10
UBB P3
BI P4
BI P9
UBB P10
ABB P10
ABB P10
BI P1
AI P1
Bannock
Medium
Interface
Growth on plate (II) after
P1
Anoxic
Oxic
324
352
Alteromonadaceae
345
Marinobacter hydrocarbonoclasticus MBIC1303
8
326
Marinobacter aqueoli
8
3
347
Marinobacter hydrocarbonoclasticus MBIC1303
325
Alteromonadaceae bacterium
327
Alteromonadaceae
7
2
Medium
Alteromonadaceae bacterium
P4
335
Idiomarina loihiensis strain 53
359
Pseudomonas pseudoalcaligenes
348
Idiomarina loihiensis strain 53
Medium
11
P9
321
Alcanivorax borkumensis
322
Alteromonas bacterium LA34A
Anoxic
Interface
Urania
Medium
359
Growth on plate (II) after
P10
Pseudomonas pseudoalcaligenes
Body Brine
Medium
P10
366
Alcanivorax venusti
365
Sulfitobacter sp. EE-36
364
Sulfitobacter sp. EE-36
Medium
P3
Anoxic
368
Uncultured Halomonas SIC.42334
4
367
Sulfitobacter sp.
1
369
Pseudomonas alcalophila
3
L'Atalante
Medium
Upper Interface
Growth on plate (II) after
Anoxic
P1
350
Alteromonadaceae bacterium
353
Marinobacter hydrocarbonoclasticus MBIC1303.
340
Alteromonas bacterium
5
342
Marinobacter hydrocarbonoclasticus MBIC1303.
2
355
Alteromonadaceae bacterium
9
Body Brine
Medium
P10
Oxic
Anoxic
361
Halomonas marina
362
Idiomarina loihiensis strain 53, Oceanospirillum
3
363
Alcanivorax venusti
4
Bannock Interface on P4
Bannock interface on P1
0,80
332
0,60
333
0,40
334
0,20
330
0,00
356
0
1
2
3
4
5
6
7
8
OD 600nm
OD 600nm
1,00
324
0,30
0,25
0,20
0,15
0,10
0,05
0,00
348
325
326
327
345
347
346
0
1
2
3
4
5
6
7
8
Time (days)
Time (days)
Bannock Body Brine on P4
0,7
0,6
OD 600nm
Growth curves of Bannock
Isolate in the culture medium
0,5
0,4
370
0,3
0,2
0,1
0
0
1
2
3
4
5
6
7
8
9
10 11 12
Time (days)
Bannock Sediment on P2
Bannock Sediment on P1
1,5
1
331
0,5
0
0
1
2
3
4
5
6
7
Time (days)
8
9
10 11 12
OD 600nm
OD 600nm
2
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
0
349
0
1
2
3
4
5
6
7
Time (days)
8
9
10 11 12
Growth curves of Urania Isolates in the culture medium
Urania Body Brine on P3
Urania Body Brine on P10 (oil)
0,5
0,30
0,25
0,20
0,15
0,10
0,05
0,00
0,2
358
359
OD 600nm
OD 600nm
0,4
367
0,3
368
0,2
369o363
0,15
364
0,1
366
0
0
1
2
3
4
5
6
7
8
0
0 1 2 3 4 5 6 7 8 9 10 11 12
Time (days)
1
2
3
4
5
6
7
8
9 10 11 12
Time (days)
Time (days)
Growth curves of L’Atalante Isolates in the culture medium
L'Atalante Body Brine on P10 (oil)
L'Atalante Interface on P1
340
1
1
341
0,8
342
0,6
343
0,4
344
350
0,2
0,8
OD 600nm
0
365
0,05
0,1
OD 600nm
Od 600nm
Urania Interface on P10
360
0,6
361
0,4
362
363o369
0,2
351
0
353
0
1
2
3
4
5
6
7
Time (days)
8
9
10 11 12
354
355
0
0
1
2
3
4
5
6
7
Time (days)
8
9 10 11 12