PEDRIGREE AND GENETIC
ANALISYS OF THE ISTRIAN
CATTLE
Ivanković A.1, Ramljak J.1, Šubara G.2, Kelava N.1,
Prekalj G.2
(1) Department of Animal Production and Technology, Agricultural Faculty, University of Zagreb,
Svetošimunska 25, 10 000 Zagreb, Croatia.
(2) AZRRI – Agency for rural development of Istria Ltd. Pazin; Agenzia per lo sviluppo rurale
dell’Istria Pisino
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
Introduction
 autochthonous cattle breeds in Croatia
 number of animals drastically declined → systematic upgrading
 1998. – 138 animals; 2008 – 460 animals
 institutional measures (subsidies, promotions), established association
(AZRRI), economic reaffirmations (brand “beef” production)
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
 Aim
– analyze of pedigree and genetic variability within Istrian Cattle →
shaping breeding programmes and preserving strategies
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
Material & Methods
 Pedigree information
• data 2 151 (1994 – 2008.)
• ENDOG v4.5 program
 Blood samples
• 51 unrelated animals
Geographical
location of Istrian
Cattle populations
• Fstat v2.9.3, GenePop 3.4.,
Bottleneck v1.2.02 program
 30 microsatellites belong to the panel of the MoDAD marker sets
recommended by FAO
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
Results
Generation interval and number of herds contributing sires (HS), grand-sires (HSS),
great-grandsires (HSSS) in Istrian cattle.
Generation interval
Number of herds
Pathway
Mean  SE
Father – son
5.803  0.396
HS
48
12.57
Father – daughter 5.811  0.363
HSS
21
4.01
Mother – son
7.313  0.375
HSSS
9
2.10
Mother –
daughter
7.222  0.389
HSSSS
5
1.47
Total
6.526  0.134
Actual
Effective
Number of alleles (MNA), observed and expected heterozygosity (Ho, HE), polymorphism
information content (PIC), fixation coefficient (FIS) at 30 microsatellites.
MNA
HO
HE
PIC
FIS
Marcatore
Locus
BM1818
7
0.765
0.708
0.673
-0.092
HEL9
9
0.647
0.663
0.641
0.034
BM1824
4
0.529
0.571
0.479
0.082
ILSTS005
2
0.098
0.13
0.122
0.241
BM2113
7
0.588
0.719
0.671
0.192**
ILSTS006
9
0.627
0.636
0.603
0.003
CSRM60
9
0.745
0.787
0.759
0.063
INRA005
3
0.569
0.658
0.584
0.146
INRA023
8
0.667
0.822
0.798
0.166**
INRA032
5
0.49
0.49
0.442
0.01
INRA035
4
0.451
0.471
0.428
0.053
Marcatore
Locus
MNA
HO
HE
PIC
FIS
CSSM66
10
0.529
0.557
0.540
0.041
ETH10
5
0.490
0.478
0.430
-0.015
ETH152
6
0.863
0.771
0.738
-0.110
INRA037
6
0.471
0.641
0.601
0.197**
ETH185
7
0.804
0.778
0.749
-0.065
INRA063
5
0.49
0.614
0.535
0.195
ETH225
7
0.333
0.791
0.761
0.275*
MM12
8
0.784
0.725
0.688
-0.072
ETH3
7
0.647
0.597
0.557
-0.074
NCAM
6
0.471
0.537
0.513
0.116
HAUT24
6
0.569
0.651
0.600
0.136
TGLA122
7
0.686
0.746
0.715
0.053
TGLA126
5
0.706
0.764
0.725
0.086
TGLA227
10
0.784
0.842
0.824
0.079
TGLA53
11
0.588
0.766
0.737
0.177
6.6
0.589
0.656
0.617
0.071***
HAUT27
6
0.353
0.605
0.579
0.025
HEL1
6
0.627
0.747
0.707
0.136*
HEL13
6
0.490
0.640
0.571
0.229
Media
HEL5
8
0.804
0.780
0.750
-0.085
Mean
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
Estimates of average inbreeding and the average relatedness in Istrian cattle
Complete
equivalent
generations
Average F (%)
in the whole
pedigree
Average
relatedness
(AR) (%)
Inbred animals
(%)
Average F (%)
of inbred
animals
1.78
2.38
3.58
8.71
16.03
low pedigree
completeness
mating
between
relatives
high degree of
weaker pedigree completeness,
relationships
more precision mating scheme
and migration
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
absence of bottleneck using Wilcoxon test (TPM)
L-shaped mode shift grah showing lack of bottleneck in Istrian cattle
population
Allele frequencies
0.6
0.5
0.4
0.3
0.2
0.1
0
1
2
3
4
5
6
Allele frequencies class
7
8
9
10
Sulle tracce delle Podoliche – On the Tracks of Grey Podolic
cattle
Conclusions

Istrian cattle → did preserve part of genetic diversity
 presence of inbreeding, with absence of bottleneck
 unsystematic pedigree data → DNA testing
 still endangered breed →
– continuous monitoring of population parameters,
– adjusting breeding program
 preserving Istrian cattle breeds have to have high national and
international priority
Acknowledgements:
We thank all breeders and Reproduction Center for providing
samples as well as Croatian Agriculture Agency.
The study of Croatian breeds was partly supported by the
Ministry of Science, Education and Sports, Croatia.