Test
sample
+
Normal recombinant
PrP
+
Thioflavin T
(ThT)
sCJD brain
1:4,000,000
sCJD nasal
brushing
96-well plate
Non-CJD nasal
brushing
Shaking fluorescence
plate reader
+
prion
seed
PrP amyloid with fluorescent ThT stain
Normal recombinant PrP
+
ThT
sCJD brain
1:4,000,000
sCJD nasal
brushing
Non-CJD nasal
brushing