VII edizione Capri Pediatria
Capri,
28 – 30 Aprile 2014
Biomarkers nell’asma bronchiale
Guglielmo Scala
Napoli
Pathogenesis of Asthma
Holgate S, Clin Exp Allergy, 2008
Pathogenesis of Asthma
Holgate S, Clin Exp Allergy, 2008
La cellula Th2 è fondamentale per la maturazione dell’eosinofilo
Pathogenesis of Asthma
Holgate S, Clin Exp Allergy, 2008
A new look at the pathogenesis of asthma
Holgate ST, 2010, Clinical Science
A new look at the pathogenesis of asthma
Holgate ST, 2010, Clinical Science
Pathogenesis of Asthma
Holgate S, Clin Exp Allergy, 2008
Nasce il
concetto di
EMTU
Lamina basale è prodotta
dalle cell epiteliali
Lamina reticolare (MBR) è
prodotta dai fibroblasti
A new look at the pathogenesis of asthma
Holgate ST, 2010, Clinical Science
Viral infections and atopy in asthma pathogenesis: new rationales for asthma prevention and treatment
P. Holt & P. Sly, Nature Medicine, 2012
Holt & Sly, Nat Med 2012
Holt & Sly, Nat Med 2012
Holt & Sly, Nat Med 2012
Holt & Sly, Nat Med 2012
Smascherata la marcia atopica??
Holt & Sly, Nat Med 2012
The basics of epithelial-mesenchymal transition
Kalluri R, Weinbergjj R , J Clin Invest. 2009.
The basics of epithelial-mesenchymal transition
Kalluri R, Weinbergjj R , J Clin Invest. 2009.
A clinical pharmacological study of the potential beneficial effects of a propolis food product as an adjuvant in
asthmatic patients.
Khayyal MT 2003, Fundam Clin Pharmacol
22 pazienti : placebo
24 pazienti : propoli
FVC
+ 19%
FEV1
+30%
PEFR
+30%
FEF 25-75
+41%
TNF
-52%
ICAM-1
-65%
IL-6
-44%
IL-8
-30%
IL-10
+300%
Asma notturno/settimana
- 60 %
Brazilian Green Propolis: Anti-Inflammatory Property by an Immunomodulatory Activity
Machado JL et al, 2012, Evidence-Based Compl Altern Med
Caffeic acid phenethyl ester inhibits nuclear factor-kB and protein kinase B signalling pathways and induces caspase3 expression in primary human CD4+ T cells
Wang et al, 2009, Clin Exp Immunol
The inhibitory effect of CAPE results in the
inhibition of cytokine production and
proliferation in primary human CD4+ T cells
Caffeic Acid Phenethyl Ester and Its Amide Analogue Are Potent Inhibitors of Leukotriene Biosynthesis in Human Polymorphonuclear
Leukocytes.
Boudreau L, 2012, PlosOne
Caffeic Acid Phenethyl Ester and Its Amide Analogue Are Potent Inhibitors of Leukotriene Biosynthesis in Human Polymorphonuclear
Leukocytes.
Boudreau L, 2012, PlosOne
Propolis inhibits TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cells via PPARγ activation.
Kao HF, et al. 2013, Int Immunopharmacol
TGF- b
Alterazioni morfologiche
↓ E - cadherin
↑ N - cadherin
↑ ROS
↑ motilità cellulare
Propolis inhibits TGF-β1-induced epithelial-mesenchymal transition in human
alveolar epithelial cells via PPARγ activation.
Kao HF, et al. 2013, Int Immunopharmacol
TGF- b
Alterazioni morfologiche
↓ E - cadherin
↑ N - cadherin
↑ ROS
PROPOLI
↑ motilità cellulare
Se pretrattiamo con CAPE
(ac. Caffeico) l’effetto è di
minore intensità.
Propolis inhibits TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cells via PPARγ activation.
Kao HF, et al. 2013, Int Immunopharmacol
The results of this study suggest that natural propolis
extracts may prevent TGF-β1-induced EMT in alveolar
cells, which may be clinically applied in the prevention
and/or treatment of EMT-related fibrotic diseases as well
as airway remodeling in chronic asthma
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